Rorug03G0013100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0007367
Physical Location & Seq
Reverse (-)
1027091 .. 1030045
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0013100.1

Sequence Viewer

Length: 1446 bp
ATGGCACTGGCTGCGCGTTCAATCTTGCTGTCCCATAATGATAGGATTGGCAGTACATGTATGAGGTTGGGATTTACAAGTGAAGAAAGGTTTCAGAAGAGAAGGATGGTTGATTTTCAAGTGGTTGTGAAATTTTGTCCCAATAGCAGAAAATCAAGATACTCCCAATTCATTAGTTCTGATAGAAACTCTTCTGGTTTGGGGTCAAAATGGTCTTCTCTGCTCTCTAGCAGGACAGAGAAGGCTTTTTTGAAAAGTGGCACAAGAACTAGTGAAACTGAGACTAAACAGGTGATTAATGTTATACTTGAAGAAATACTCAGCTTCATTAAAGAGTCCAGTCAGTTTCACTTCATGAAAGTAGCTGGTTTACTGGTATGCACTCTCATGGTGATTCCATCAGCCAATGCTGTCGATGCTCTCAAAACTTGTTCTTGCTTACTTAAGGAATGCAGGGTAGAACTTGCAAAGTGCATTTCAAACCCGTCCTGTGCTGCCAATGTTGCTTGTCTGCAAACTTGTAACAATCGACCTGATGAGACGGAATGCCAGATTAAATGTGGGGACCTGTTTGAGAACAGTGTCGTGGATGAATTTAATGAATGTGCAGTGTCACGAAAGAAGTGTGTACCTATGAAATCCGATGTGGGAGAATTTCCTATCCCAGATCCTTCTGTTCTTGTTCAAAGCTTTGATATGGAAAAGTTTAATGGGAAATGGTTCATTACTAGTGGCTTGAATCCTACCTTTGATGCTTTTGATTGCCAAGTGCACGAATTCCATACAGAATCTGGCAGACTTGTAGGAAATTTGTCTTGGAGAATAAGAACCCCCGATGGCGGATTTTTTACTCGAACAGCTGTTCAGAAATTCGTGCAAGATCCAAACCAGCCTGGCATACTATACAATCACGACAATGATTATCTTCACTATCAAGATGACTGGTACATTCTATCTTCAAAGATAGAGAATAAACCGGAAGACTATATATTTGTATACTACCGTGGCAGGAATGATGCGTGGGATGGATACGGTGGTGCTGTGCTATATACAAGGAGTTCAGTTTTGCCACAAAGCATTGTTCCTGATCTTGAAAAAGCAGCTAAGAGCGTGGGACGAGACTTCAGCAAGTTTATCAAAACAGATAATACTTGCGGGCCTGAACCTCCACTTGTAGAGAGGTTGGAGAAGACACTTGAGCAAGGGGAGAGGACCATTATAGAGGAAGTTCAACAGATAGAAGGGGAAGTGGAGAAGGTTGAACAGACAGAGCGGTCATTGTTTCAAAAGTTAGCAGAGGGGTTTAATGAGATTAAACAAGATGAGGAGAACTTCTTGAGAGAGCTTTCCAAAGAAGAGATGGATATATTGAGCGAGCTCAAAATGGAAGCAACCGAAGTAGAAAAGCTTTTCGGACGAACACTTCCTCTTCGGAAGCTTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

481

Amino Acids

54.77

Weight (kDa)

5.5

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VDE PF07137 144 - 383 1.4e-108 VDE lipocalin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1273
AccBSI CCGCTC 1 cut(s) 1273
AccI GTMKAC 1 cut(s) 996
AccII CGCG 1 cut(s) 16
AciI CCGC 3 cut(s) 840, 1155, 1273
AclWI GGATC 2 cut(s) 662, 875
AcsI RAATTY 6 cut(s) 131, 593, 653, 776, 808, 869
AcuI CTGAAG 1 cut(s) 1108
AfaI GTAC 3 cut(s) 55, 630, 947
AfiI CCNNNNNNNGG 1 cut(s) 839
AflII CTTAAG 1 cut(s) 443
AflIII ACRYGT 1 cut(s) 56
AhlI ACTAGT 2 cut(s) 269, 728
AjnI CCWGG 1 cut(s) 892
AjuI GAANNNNNNNTTGG 2 cut(s) 799, 831
AluBI AGCT 9 cut(s) 324, 365, 690, 860, 1103, 1345, 1378, 1408, 1438
AluI AGCT 9 cut(s) 324, 365, 690, 860, 1103, 1345, 1378, 1408, 1438
Alw21I GWGCWC 2 cut(s) 774, 1380
Alw26I GTCTC 3 cut(s) 275, 533, 1113
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 2 cut(s) 662, 875
AlwNI CAGNNNCTG 1 cut(s) 791
AoxI GGCC 1 cut(s) 1157
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 3 cut(s) 11, 494, 1100
ApoI RAATTY 6 cut(s) 131, 593, 653, 776, 808, 869
ArsI GACNNNNNNTTYG 2 cut(s) 974, 1006
AseI ATTAAT 1 cut(s) 297
Asp700I GAANNNNTTC 3 cut(s) 90, 190, 719
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 3 cut(s) 565, 1157, 1212
AsuHPI GGTGA 2 cut(s) 304, 403
AvaII GGWCC 2 cut(s) 565, 1212
BaeGI GKGCMC 1 cut(s) 774
BaeI ACNNNNGTAYC 2 cut(s) 1021, 1054
BanII GRGCYC 1 cut(s) 1380
BbsI GAAGAC 3 cut(s) 207, 987, 1196
Bbv12I GWGCWC 2 cut(s) 774, 1380
BbvI GCAGC 2 cut(s) 481, 1112
BccI CCATC 5 cut(s) 100, 406, 830, 1019, 1354
BciT130I CCWGG 1 cut(s) 894
BciVI GTATCC 1 cut(s) 1022
BcoDI GTCTC 3 cut(s) 275, 533, 1113
BcuI ACTAGT 2 cut(s) 269, 728
BfaI CTAG 3 cut(s) 228, 270, 729
BfrI CTTAAG 1 cut(s) 443
BfuI GTATCC 1 cut(s) 1022
BisI GCNGC 3 cut(s) 12, 495, 1101
BlsI GCNGC 3 cut(s) 13, 496, 1102
Bme1390I CCNGG 1 cut(s) 894
Bme18I GGWCC 2 cut(s) 565, 1212
BmgT120I GGNCC 3 cut(s) 565, 1157, 1212
BmiI GGNNCC 1 cut(s) 566
BmrFI CCNGG 1 cut(s) 894
BmsI GCATC 3 cut(s) 406, 742, 1006
BpiI GAAGAC 3 cut(s) 207, 987, 1196
BpuEI CTTGAG 2 cut(s) 1217, 1357
BsaJI CCNNGG 1 cut(s) 1003
BsaWI WCCGGW 1 cut(s) 976
Bsc4I CCNNNNNNNGG 1 cut(s) 839
Bse1I ACTGG 4 cut(s) 12, 339, 378, 947
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 1 cut(s) 1003
BseGI GGATG 3 cut(s) 111, 595, 1030
BseLI CCNNNNNNNGG 1 cut(s) 839
BseMII CTCAG 2 cut(s) 270, 334
BseNI ACTGG 4 cut(s) 12, 339, 378, 947
BseRI GAGGAG 1 cut(s) 1340
BseSI GKGCMC 1 cut(s) 774
BseXI GCAGC 2 cut(s) 481, 1112
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 1 cut(s) 16
BshFI GGCC 1 cut(s) 1159
BsiHKAI GWGCWC 2 cut(s) 774, 1380
BsiSI CCGG 1 cut(s) 977
BslFI GGGAC 4 cut(s) 16, 123, 578, 1128
BslI CCNNNNNNNGG 1 cut(s) 839
BsmAI GTCTC 3 cut(s) 275, 533, 1113
BsmBI CGTCTC 1 cut(s) 533
BsmFI GGGAC 4 cut(s) 16, 123, 578, 1128
BsmI GAATGC 2 cut(s) 455, 551
BsnI GGCC 1 cut(s) 1159
Bsp1286I GDGCHC 2 cut(s) 774, 1380
Bsp143I GATC 3 cut(s) 667, 880, 1087
BspACI CCGC 3 cut(s) 840, 1155, 1273
BspANI GGCC 1 cut(s) 1159
BspCNI CTCAG 2 cut(s) 271, 333
BspFNI CGCG 1 cut(s) 16
BspHI TCATGA 1 cut(s) 354
BspLI GGNNCC 1 cut(s) 566
BspPI GGATC 2 cut(s) 662, 875
BspTI CTTAAG 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 1273
BsrI ACTGG 4 cut(s) 12, 339, 378, 947
BssECI CCNNGG 1 cut(s) 1003
BssMI GATC 3 cut(s) 667, 880, 1087
BssNAI GTATAC 1 cut(s) 997
Bst1107I GTATAC 1 cut(s) 997
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 3 cut(s) 581, 1004, 1034
Bst6I CTCTTC 4 cut(s) 92, 196, 1350, 1434
BstAFI CTTAAG 1 cut(s) 443
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 1157, 1376
BstDEI CTNAG 4 cut(s) 279, 320, 1104, 1439
BstDSI CCRYGG 1 cut(s) 1003
BstF5I GGATG 3 cut(s) 111, 595, 1030
BstFNI CGCG 1 cut(s) 16
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 3 cut(s) 670, 883, 1090
BstMAI GTCTC 3 cut(s) 275, 533, 1113
BstMBI GATC 3 cut(s) 667, 880, 1087
BstMWI GCNNNNNNNGC 3 cut(s) 11, 416, 503
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 1 cut(s) 60
BstSCI CCNGG 1 cut(s) 892
BstSLI GKGCMC 1 cut(s) 774
BstUI CGCG 1 cut(s) 16
BstV1I GCAGC 2 cut(s) 481, 1112
BstV2I GAAGAC 3 cut(s) 207, 987, 1196
BstX2I RGATCY 2 cut(s) 667, 880
BstYI RGATCY 2 cut(s) 667, 880
BstZ17I GTATAC 1 cut(s) 997
BsuI GTATCC 1 cut(s) 1022
BsuRI GGCC 1 cut(s) 1159
BtgI CCRYGG 1 cut(s) 1003
BtsCI GGATG 3 cut(s) 111, 595, 1030
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 5, 586, 615
Cac8I GCNNGC 2 cut(s) 1157, 1376
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 1 cut(s) 354
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 3 cut(s) 565, 1157, 1212
Csp6I GTAC 3 cut(s) 54, 629, 946
CviAII CATG 3 cut(s) 57, 355, 388
CviQI GTAC 3 cut(s) 54, 629, 946
DdeI CTNAG 4 cut(s) 279, 320, 1104, 1439
DpnI GATC 3 cut(s) 669, 882, 1089
DpnII GATC 3 cut(s) 667, 880, 1087
DrdI GACNNNNNNGTC 1 cut(s) 1273
DseDI GACNNNNNNGTC 1 cut(s) 1273
Eam1104I CTCTTC 4 cut(s) 92, 196, 1350, 1434
EarI CTCTTC 4 cut(s) 92, 196, 1350, 1434
EciI GGCGGA 1 cut(s) 855
Ecl136II GAGCTC 1 cut(s) 1378
Eco24I GRGCYC 1 cut(s) 1380
Eco47I GGWCC 2 cut(s) 565, 1212
Eco53kI GAGCTC 1 cut(s) 1378
Eco57I CTGAAG 1 cut(s) 1108
EcoICRI GAGCTC 1 cut(s) 1378
EcoO109I RGGNCCY 1 cut(s) 565
EcoRI GAATTC 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 1380
Esp3I CGTCTC 1 cut(s) 533
FaeI CATG 3 cut(s) 60, 358, 391
FaqI GGGAC 4 cut(s) 16, 123, 578, 1128
FatI CATG 3 cut(s) 56, 354, 387
FauI CCCGC 1 cut(s) 1148
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 3 cut(s) 12, 495, 1101
FokI GGATG 3 cut(s) 118, 602, 1037
FriOI GRGCYC 1 cut(s) 1380
Fsp4HI GCNGC 3 cut(s) 12, 495, 1101
FspBI CTAG 3 cut(s) 228, 270, 729
GlaI GCGC 1 cut(s) 15
GluI GCNGC 3 cut(s) 12, 495, 1101
HaeIII GGCC 1 cut(s) 1159
HapII CCGG 1 cut(s) 977
HhaI GCGC 1 cut(s) 16
Hin1II CATG 3 cut(s) 60, 358, 391
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 3 cut(s) 688, 1406, 1436
HinfI GANTC 4 cut(s) 335, 394, 739, 788
HpaII CCGG 1 cut(s) 977
HphI GGTGA 2 cut(s) 304, 403
Hpy166II GTNNAC 4 cut(s) 371, 629, 772, 997
Hpy188I TCNGA 6 cut(s) 96, 181, 643, 867, 1415, 1434
Hpy188III TCNNGA 8 cut(s) 156, 355, 615, 911, 935, 1085, 1091, 1336
Hpy8I GTNNAC 4 cut(s) 371, 629, 772, 997
HpyAV CCTTC 5 cut(s) 96, 235, 681, 1235, 1249
HpyCH4III ACNGT 3 cut(s) 581, 1004, 1034
HpyCH4V TGCA 8 cut(s) 381, 453, 467, 474, 514, 608, 772, 877
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 416, 503
HpyF3I CTNAG 4 cut(s) 279, 320, 1104, 1439
Hsp92II CATG 3 cut(s) 60, 358, 391
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 3 cut(s) 667, 880, 1087
Lsp1109I GCAGC 2 cut(s) 481, 1112
LweI GCATC 3 cut(s) 406, 742, 1006
MaeI CTAG 3 cut(s) 228, 270, 729
MaeIII GTNAC 2 cut(s) 521, 612
MalI GATC 3 cut(s) 669, 882, 1089
MbiI CCGCTC 1 cut(s) 1273
MboI GATC 3 cut(s) 667, 880, 1087
MflI RGATCY 2 cut(s) 667, 880
MhlI GDGCHC 2 cut(s) 774, 1380
MluCI AATT 7 cut(s) 131, 167, 593, 653, 776, 808, 869
MlyI GAGTC 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 1164
MnlI CCTC 8 cut(s) 57, 1173, 1176, 1203, 1216, 1291, 1318, 1437
MroXI GAANNNNTTC 3 cut(s) 90, 190, 719
MseI TTAA 8 cut(s) 297, 330, 444, 555, 597, 708, 1305, 1314
MslI CAYNNNNRTG 2 cut(s) 386, 915
MspA1I CMGCKG 1 cut(s) 860
MspCI CTTAAG 1 cut(s) 443
MspI CCGG 1 cut(s) 977
MspR9I CCNGG 1 cut(s) 894
Mva1269I GAATGC 2 cut(s) 455, 551
MvaI CCWGG 1 cut(s) 894
MvnI CGCG 1 cut(s) 16
MwoI GCNNNNNNNGC 3 cut(s) 11, 416, 503
NdeII GATC 3 cut(s) 667, 880, 1087
NlaIII CATG 3 cut(s) 60, 358, 391
NlaIV GGNNCC 1 cut(s) 566
NmuCI GTSAC 1 cut(s) 612
NspI RCATGY 1 cut(s) 60
PagI TCATGA 1 cut(s) 354
PciI ACATGT 1 cut(s) 56
PctI GAATGC 2 cut(s) 455, 551
PdmI GAANNNNTTC 3 cut(s) 90, 190, 719
PfeI GAWTC 3 cut(s) 394, 739, 788
PkrI GCNGC 3 cut(s) 13, 496, 1102
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PpuMI RGGWCCY 1 cut(s) 565
PscI ACATGT 1 cut(s) 56
PshBI ATTAAT 1 cut(s) 297
Psp124BI GAGCTC 1 cut(s) 1380
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 1 cut(s) 892
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 1 cut(s) 566
PspPI GGNCC 3 cut(s) 565, 1157, 1212
PspPPI RGGWCCY 1 cut(s) 565
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 2 cut(s) 667, 880
PvuII CAGCTG 1 cut(s) 860
RsaI GTAC 3 cut(s) 55, 630, 947
RsaNI GTAC 3 cut(s) 54, 629, 946
RseI CAYNNNNRTG 2 cut(s) 386, 915
SacI GAGCTC 1 cut(s) 1380
SaqAI TTAA 8 cut(s) 297, 330, 444, 555, 597, 708, 1305, 1314
SatI GCNGC 3 cut(s) 12, 495, 1101
Sau3AI GATC 3 cut(s) 667, 880, 1087
Sau96I GGNCC 3 cut(s) 565, 1157, 1212
SchI GAGTC 1 cut(s) 344
ScrFI CCNGG 1 cut(s) 894
SduI GDGCHC 2 cut(s) 774, 1380
SfaNI GCATC 3 cut(s) 406, 742, 1006
SinI GGWCC 2 cut(s) 565, 1212
SmiMI CAYNNNNRTG 2 cut(s) 386, 915
SmlI CTYRAG 3 cut(s) 443, 1196, 1336
SmoI CTYRAG 3 cut(s) 443, 1196, 1336
SpeI ACTAGT 2 cut(s) 269, 728
Sse9I AATT 7 cut(s) 131, 167, 593, 653, 776, 808, 869
SsiI CCGC 3 cut(s) 840, 1155, 1273
SspMI CTAG 3 cut(s) 228, 270, 729
SstI GAGCTC 1 cut(s) 1380
StyD4I CCNGG 1 cut(s) 892
TaaI ACNGT 3 cut(s) 581, 1004, 1034
TaqI TCGA 3 cut(s) 414, 529, 853
TasI AATT 7 cut(s) 131, 167, 593, 653, 776, 808, 869
TatI WGTACW 1 cut(s) 53
TfiI GAWTC 3 cut(s) 394, 739, 788
Tru1I TTAA 8 cut(s) 297, 330, 444, 555, 597, 708, 1305, 1314
Tru9I TTAA 8 cut(s) 297, 330, 444, 555, 597, 708, 1305, 1314
TscAI CASTG 3 cut(s) 12, 586, 615
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 3 cut(s) 11, 494, 1100
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 8 cut(s) 160, 316, 343, 371, 606, 615, 650, 712
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 3 cut(s) 12, 586, 615
Vha464I CTTAAG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 770
VpaK11BI GGWCC 2 cut(s) 565, 1212
VspI ATTAAT 1 cut(s) 297
XapI RAATTY 6 cut(s) 131, 593, 653, 776, 808, 869
XceI RCATGY 1 cut(s) 60
XcmI CCANNNNNNNNNTGG 3 cut(s) 557, 788, 1357
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 3 cut(s) 90, 190, 719
XspI CTAG 3 cut(s) 228, 270, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.