AT3G05625

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007367
Physical Location & Seq
Forward (+)
1632213 .. 1634205
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05625.2

Sequence Viewer

Length: 774 bp
ATGTCACTAACCCAGATCGTGAATCCAATCATATATCCATCTCGCACAATTCCACGGCGTTTCACGGCGATCTCGCCTTTTAAAACCCTAAGTCCCCAATTTTCCCGCCACCCACCTCACGCACTGTCTCGTAGACTCTTCCTTCCTTCTGTTTCCTCTATATGGGATGCCATAACCGGCGGCGGAGATTCGAACCCTCGTGAGGCCATCGCAGCGGTTCGACGCGGAATGCAGCTCTTCAGACAGGGTGATGTTGCTGGATCGGTGGCAGAGTTCGATAGAGCAATCATTTTGGATCCCAGGCAAAAGGCATATCTTTGGCAACGCGGGCTTTCTCTTTACTATGTAGATAGGTTTGAAGAAGGTGCAGAGCAGTTCCGGATAGATGTTGCTCAGAATCCTAATGACACAGAGGAGTCCATTTGGTGCTTTATCTGCGAGGCGAGGCTCCATGGTGTTGATGTTGCACGAACACAGTTTCTCGAGGTTGGTAGAGATTCTCGGCCTGTGATGCGAGAAGCTTATAATCTATTCAAGAATGGTGGTGATCCTGAAAAGTTAGTAAATGACTTCTCTAGCGGTCAAGCAAGCGAATACTTCTATGCTTCTCTGTACGCAGGGCTCTACTATGAAGCTGAGGGTAAATCAGAAAACGCAAAGTTTCATTTAACCGCGGCTTGTGGATCTCCATATGGGCAGAGGTCTGATGATTATATGGCTTCACTTGCTAAAGTCCATTGCCTTTGTAGAAACTGGAGCTCCGGTATTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

28.95

Weight (kDa)

6.96

Isoelectric Point (pI)

48.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccI GTMKAC 1 cut(s) 133
AccII CGCG 3 cut(s) 225, 327, 674
AccIII TCCGGA 1 cut(s) 378
AciI CCGC 9 cut(s) 106, 180, 183, 215, 225, 327, 579, 672, 674
AclWI GGATC 5 cut(s) 268, 290, 303, 542, 691
AcuI CTGAAG 1 cut(s) 223
AfaI GTAC 1 cut(s) 614
AfiI CCNNNNNNNGG 2 cut(s) 162, 202
AgsI TTSAA 2 cut(s) 359, 535
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 4 cut(s) 235, 521, 635, 759
AluI AGCT 4 cut(s) 235, 521, 635, 759
Alw21I GWGCWC 1 cut(s) 761
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 5 cut(s) 268, 290, 303, 542, 691
Ama87I CYCGRG 1 cut(s) 482
Aor13HI TCCGGA 1 cut(s) 378
AoxI GGCC 2 cut(s) 204, 503
ApeKI GCWGC 2 cut(s) 212, 232
AsuHPI GGTGA 2 cut(s) 260, 557
AsuII TTCGAA 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 482
BamHI GGATCC 1 cut(s) 295
BanII GRGCYC 2 cut(s) 624, 761
BauI CACGAG 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 761
BbvCI CCTCAGC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 224, 244
BccI CCATC 2 cut(s) 46, 215
BceAI ACGGC 2 cut(s) 71, 81
BciT130I CCWGG 1 cut(s) 301
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 576
BisI GCNGC 4 cut(s) 181, 213, 233, 675
BlsI GCNGC 4 cut(s) 182, 214, 234, 676
Bme1390I CCNGG 1 cut(s) 301
BmeT110I CYCGRG 1 cut(s) 482
BmiI GGNNCC 2 cut(s) 297, 449
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 2 cut(s) 157, 501
Bpu10I CCTNAGC 1 cut(s) 636
Bpu14I TTCGAA 1 cut(s) 191
BsaJI CCNNGG 4 cut(s) 53, 299, 451, 672
BsaWI WCCGGW 2 cut(s) 378, 761
BsaXI ACNNNNNCTCC 3 cut(s) 743, 748, 773
Bsc4I CCNNNNNNNGG 2 cut(s) 162, 202
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 758
Bse3DI GCAATG 1 cut(s) 736
BseAI TCCGGA 1 cut(s) 378
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 4 cut(s) 53, 299, 451, 672
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 2 cut(s) 162, 202
BseMI GCAATG 1 cut(s) 736
BseMII CTCAG 2 cut(s) 407, 627
BseNI ACTGG 1 cut(s) 758
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 2 cut(s) 224, 244
BsgI GTGCAG 1 cut(s) 387
Bsh1236I CGCG 3 cut(s) 225, 327, 674
BshFI GGCC 2 cut(s) 206, 505
BsiHKAI GWGCWC 1 cut(s) 761
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 3 cut(s) 177, 379, 762
BslFI GGGAC 1 cut(s) 78
BslI CCNNNNNNNGG 2 cut(s) 162, 202
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 78
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 2 cut(s) 206, 505
BsoBI CYCGRG 1 cut(s) 482
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 2 cut(s) 624, 761
Bsp13I TCCGGA 1 cut(s) 378
Bsp143I GATC 6 cut(s) 15, 69, 260, 295, 547, 683
Bsp19I CCATGG 1 cut(s) 451
BspACI CCGC 9 cut(s) 106, 180, 183, 215, 225, 327, 579, 672, 674
BspANI GGCC 2 cut(s) 206, 505
BspCNI CTCAG 2 cut(s) 406, 628
BspEI TCCGGA 1 cut(s) 378
BspFNI CGCG 3 cut(s) 225, 327, 674
BspLI GGNNCC 2 cut(s) 297, 449
BspPI GGATC 5 cut(s) 268, 290, 303, 542, 691
BspQI GCTCTTC 1 cut(s) 242
BspT104I TTCGAA 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 736
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 758
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 4 cut(s) 53, 299, 451, 672
BssMI GATC 6 cut(s) 15, 69, 260, 295, 547, 683
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 451
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 2 cut(s) 126, 477
Bst6I CTCTTC 2 cut(s) 143, 242
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 329, 589
BstDEI CTNAG 3 cut(s) 89, 393, 636
BstDSI CCRYGG 3 cut(s) 53, 451, 672
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 3 cut(s) 225, 327, 674
BstKTI GATC 6 cut(s) 18, 72, 263, 298, 550, 686
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 6 cut(s) 15, 69, 260, 295, 547, 683
BstMWI GCNNNNNNNGC 5 cut(s) 212, 328, 435, 511, 725
BstNI CCWGG 1 cut(s) 301
BstSCI CCNGG 1 cut(s) 299
BstUI CGCG 3 cut(s) 225, 327, 674
BstV1I GCAGC 2 cut(s) 224, 244
BstX2I RGATCY 2 cut(s) 295, 683
BstYI RGATCY 2 cut(s) 295, 683
BsuRI GGCC 2 cut(s) 206, 505
BtgI CCRYGG 3 cut(s) 53, 451, 672
BtgZI GCGATG 1 cut(s) 193
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 329, 589
Cfr10I RCCGGY 1 cut(s) 176
Cfr42I CCGCGG 1 cut(s) 675
CseI GACGC 1 cut(s) 231
Csp6I GTAC 1 cut(s) 613
CspCI CAANNNNNGTGG 2 cut(s) 523, 558
CviAII CATG 1 cut(s) 452
CviQI GTAC 1 cut(s) 613
DdeI CTNAG 3 cut(s) 89, 393, 636
DpnI GATC 6 cut(s) 17, 71, 262, 297, 549, 685
DpnII GATC 6 cut(s) 15, 69, 260, 295, 547, 683
DraI TTTAAA 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 143, 242
EarI CTCTTC 2 cut(s) 143, 242
EciI GGCGGA 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 759
Eco130I CCWWGG 1 cut(s) 451
Eco24I GRGCYC 2 cut(s) 624, 761
Eco53kI GAGCTC 1 cut(s) 759
Eco57I CTGAAG 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 482
EcoICRI GAGCTC 1 cut(s) 759
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 451
EcoT38I GRGCYC 2 cut(s) 624, 761
ErhI CCWWGG 1 cut(s) 451
FaeI CATG 1 cut(s) 455
FaqI GGGAC 1 cut(s) 78
FatI CATG 1 cut(s) 451
FauI CCCGC 2 cut(s) 113, 320
FauNDI CATATG 1 cut(s) 691
FblI GTMKAC 1 cut(s) 133
Fnu4HI GCNGC 4 cut(s) 181, 213, 233, 675
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 2 cut(s) 624, 761
Fsp4HI GCNGC 4 cut(s) 181, 213, 233, 675
FspBI CTAG 1 cut(s) 576
GluI GCNGC 4 cut(s) 181, 213, 233, 675
HaeIII GGCC 2 cut(s) 206, 505
HapII CCGG 3 cut(s) 177, 379, 762
HgaI GACGC 1 cut(s) 231
Hin1II CATG 1 cut(s) 455
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 6 cut(s) 22, 135, 188, 397, 416, 497
HpaII CCGG 3 cut(s) 177, 379, 762
HphI GGTGA 2 cut(s) 260, 557
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 4 cut(s) 242, 396, 649, 706
Hpy188III TCNNGA 6 cut(s) 19, 200, 379, 482, 535, 551
Hpy8I GTNNAC 1 cut(s) 134
Hpy99I CGWCG 1 cut(s) 225
HpyAV CCTTC 3 cut(s) 152, 156, 356
HpyCH4III ACNGT 2 cut(s) 126, 477
HpyCH4V TGCA 3 cut(s) 232, 368, 467
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 328, 435, 511, 725
HpyF3I CTNAG 3 cut(s) 89, 393, 636
Hsp92II CATG 1 cut(s) 455
Kpn2I TCCGGA 1 cut(s) 378
KspI CCGCGG 1 cut(s) 675
Kzo9I GATC 6 cut(s) 15, 69, 260, 295, 547, 683
LguI GCTCTTC 1 cut(s) 242
LmnI GCTCC 3 cut(s) 453, 756, 764
Lsp1109I GCAGC 2 cut(s) 224, 244
LweI GCATC 2 cut(s) 157, 501
MaeI CTAG 1 cut(s) 576
MaeIII GTNAC 1 cut(s) 3
MalI GATC 6 cut(s) 17, 71, 262, 297, 549, 685
MboI GATC 6 cut(s) 15, 69, 260, 295, 547, 683
MboII GAAGA 3 cut(s) 130, 229, 371
MflI RGATCY 2 cut(s) 295, 683
MhlI GDGCHC 2 cut(s) 624, 761
MluCI AATT 2 cut(s) 48, 98
MlyI GAGTC 2 cut(s) 129, 425
MroI TCCGGA 1 cut(s) 378
MseI TTAA 2 cut(s) 81, 668
MspA1I CMGCKG 2 cut(s) 215, 674
MspI CCGG 3 cut(s) 177, 379, 762
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 3 cut(s) 225, 327, 674
MwoI GCNNNNNNNGC 5 cut(s) 212, 328, 435, 511, 725
NcoI CCATGG 1 cut(s) 451
NdeI CATATG 1 cut(s) 691
NdeII GATC 6 cut(s) 15, 69, 260, 295, 547, 683
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 2 cut(s) 297, 449
NmeAIII GCCGAG 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 3
NspV TTCGAA 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 482
PciSI GCTCTTC 1 cut(s) 242
PctI GAATGC 1 cut(s) 234
PfeI GAWTC 4 cut(s) 22, 188, 397, 497
PkrI GCNGC 4 cut(s) 182, 214, 234, 676
PleI GAGTC 2 cut(s) 129, 424
PpsI GAGTC 2 cut(s) 129, 424
PsiI TTATAA 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 297, 449
PsuI RGATCY 2 cut(s) 295, 683
RsaI GTAC 1 cut(s) 614
RsaNI GTAC 1 cut(s) 613
SacI GAGCTC 1 cut(s) 761
SacII CCGCGG 1 cut(s) 675
SapI GCTCTTC 1 cut(s) 242
SaqAI TTAA 2 cut(s) 81, 668
SatI GCNGC 4 cut(s) 181, 213, 233, 675
Sau3AI GATC 6 cut(s) 15, 69, 260, 295, 547, 683
SchI GAGTC 2 cut(s) 129, 425
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 2 cut(s) 624, 761
SetI ASST 9 cut(s) 118, 237, 356, 367, 489, 523, 637, 704, 761
SfaNI GCATC 2 cut(s) 157, 501
Sfr274I CTCGAG 1 cut(s) 482
Sfr303I CCGCGG 1 cut(s) 675
SfuI TTCGAA 1 cut(s) 191
SgrBI CCGCGG 1 cut(s) 675
SlaI CTCGAG 1 cut(s) 482
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 2 cut(s) 48, 98
SsiI CCGC 9 cut(s) 106, 180, 183, 215, 225, 327, 579, 672, 674
SspMI CTAG 1 cut(s) 576
SstI GAGCTC 1 cut(s) 761
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 126, 477
TaqI TCGA 4 cut(s) 191, 220, 276, 483
TasI AATT 2 cut(s) 48, 98
TauI GCSGC 2 cut(s) 183, 677
TfiI GAWTC 4 cut(s) 22, 188, 397, 497
Tru1I TTAA 2 cut(s) 81, 668
Tru9I TTAA 2 cut(s) 81, 668
TscAI CASTG 1 cut(s) 129
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 212, 232
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 645, 653
TspRI CASTG 1 cut(s) 129
XhoI CTCGAG 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 133
XspI CTAG 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.