Rw3G006280

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007714
Physical Location & Seq
Reverse (-)
5954394 .. 5956198
1805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006280.1

Sequence Viewer

Length: 807 bp
ATGAGCTTGGAGGATTTGCCCCAAGACTGCTTCGAGCAGATTTTATCATTGACATCTCCCGGAGATGCATGTCGTTCCTCGTTAGTCTCGTTGTCGATTCGTGCCATGGCCGATATGGATAGTTTGTGGGGTATGTTTTTGCCGTCGGACTATCCAGAAATTCTGTCCAGATTAGTCACTCCAATACCTTATTCATCCAACAAGGACTTGTTCATCAGGTTGTGCAGCCCAAATCTAATCGATTGTGGTAAAAAGATGTTCTCAATAGAGAAATCAACAGGTAAAAAATGTTACATGTTAAGTGCAAGGGATCTTTCAATTACATGGGCGTGCAATCCTCTCTATTGGAGTTGGAAGTGTTCCTCCGAATCAAGATTTGCAGAGGTTGCTGAACTCAGAACCATCTGGTGGCTAGAAATATGTGGCACCATGAATAGTCAATTGCTATCACCAAAAACAGTTTATAGTGCTCATCTTGTTATCAAGTTGGCCAATCGTGCATATGGTTTGGACTCATTACCTTCTGAGATTTCACTTGAAGTTGGCAATTACAAATCACAAGGCACTGTATACTTGAGTGCAAATCGGAAGACAACAGAAGTAGACGAACGTGTGAGACCAATCAGGGTTCTGGAAGAACGTCTTCGTGAGCGAACAGATGGATGGATGGAGATTGAACTGGGGAGCTTTTACAATGATGGATGTTATTATAACAAGGATGTAAAGATGAGCCTAAAAGAAGTTAAGGGTGCACACCTAAAAGGTGGCCTTATTGTTGAGGGAATTGAGATTAGGCCAAAAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0030246
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

268

Amino Acids

30.43

Weight (kDa)

6.22

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 95 - 266 3.4e-54 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 711
AccB1I GGYRCC 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 408
AccI GTMKAC 2 cut(s) 570, 603
AclWI GGATC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 108, 489
AcsI RAATTY 1 cut(s) 159
AfiI CCNNNNNNNGG 1 cut(s) 408
AflIII ACRYGT 2 cut(s) 294, 610
AgsI TTSAA 3 cut(s) 318, 539, 677
AjuI GAANNNNNNNTTGG 2 cut(s) 175, 207
AluBI AGCT 2 cut(s) 6, 687
AluI AGCT 2 cut(s) 6, 687
Alw21I GWGCWC 2 cut(s) 472, 754
Alw26I GTCTC 2 cut(s) 91, 610
Alw44I GTGCAC 1 cut(s) 750
AlwI GGATC 1 cut(s) 318
AoxI GGCC 4 cut(s) 108, 489, 766, 794
ApaLI GTGCAC 1 cut(s) 750
ApeKI GCWGC 1 cut(s) 225
ApoI RAATTY 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 642
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 1 cut(s) 441
BaeGI GKGCMC 1 cut(s) 754
BalI TGGCCA 1 cut(s) 491
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 2 cut(s) 596, 635
Bbv12I GWGCWC 2 cut(s) 472, 754
BbvI GCAGC 1 cut(s) 237
BccI CCATC 5 cut(s) 410, 653, 657, 661, 692
BceAI ACGGC 1 cut(s) 127
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 2 cut(s) 91, 610
BfaI CTAG 1 cut(s) 413
BisI GCNGC 1 cut(s) 226
BlsI GCNGC 1 cut(s) 227
Bme1390I CCNGG 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 60
BmrI ACTGGG 1 cut(s) 689
BmsI GCATC 1 cut(s) 55
BmuI ACTGGG 1 cut(s) 689
BpiI GAAGAC 2 cut(s) 596, 635
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 60
Bsa29I ATCGAT 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 610
BsaJI CCNNGG 1 cut(s) 105
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 684
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 105
BseGI GGATG 5 cut(s) 194, 668, 672, 707, 724
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 409, 516
BseNI ACTGG 1 cut(s) 684
BseSI GKGCMC 1 cut(s) 754
BseXI GCAGC 1 cut(s) 237
BsgI GTGCAG 1 cut(s) 244
BshFI GGCC 4 cut(s) 110, 491, 768, 796
BshNI GGYRCC 1 cut(s) 425
BshVI ATCGAT 1 cut(s) 240
BsiHKAI GWGCWC 2 cut(s) 472, 754
BsiSI CCGG 1 cut(s) 60
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 2 cut(s) 91, 610
BsnI GGCC 4 cut(s) 110, 491, 768, 796
Bso31I GGTCTC 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 472, 754
Bsp143I GATC 1 cut(s) 310
Bsp19I CCATGG 1 cut(s) 105
BspANI GGCC 4 cut(s) 110, 491, 768, 796
BspCNI CTCAG 2 cut(s) 408, 517
BspDI ATCGAT 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 427
BspPI GGATC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 610
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 1 cut(s) 105
BssMI GATC 1 cut(s) 310
BssNAI GTATAC 1 cut(s) 571
BssT1I CCWWGG 1 cut(s) 105
Bst1107I GTATAC 1 cut(s) 571
Bst4CI ACNGT 2 cut(s) 460, 568
BstAPI GCANNNNNTGC 1 cut(s) 386
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 2 cut(s) 395, 525
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 5 cut(s) 194, 668, 672, 707, 724
BstKTI GATC 1 cut(s) 313
BstMAI GTCTC 2 cut(s) 91, 610
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 2 cut(s) 386, 497
BstNSI RCATGY 2 cut(s) 72, 298
BstSCI CCNGG 1 cut(s) 58
BstSLI GKGCMC 1 cut(s) 754
BstV1I GCAGC 1 cut(s) 237
BstV2I GAAGAC 2 cut(s) 596, 635
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZ17I GTATAC 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 240
BsuRI GGCC 4 cut(s) 110, 491, 768, 796
BsuTUI ATCGAT 1 cut(s) 240
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 5 cut(s) 194, 668, 672, 707, 724
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 331
ClaI ATCGAT 1 cut(s) 240
CviAII CATG 5 cut(s) 69, 106, 295, 324, 430
CviJI RGCY 9 cut(s) 6, 110, 228, 412, 491, 687, 732, 768, 796
CviKI_1 RGCY 9 cut(s) 6, 110, 228, 412, 491, 687, 732, 768, 796
DdeI CTNAG 2 cut(s) 395, 525
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
EaeI YGGCCR 2 cut(s) 108, 489
Eco130I CCWWGG 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 105
EcoT22I ATGCAT 1 cut(s) 70
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 5 cut(s) 72, 109, 298, 327, 433
FalI AAGNNNNNCTT 4 cut(s) 627, 659, 753, 785
FatI CATG 5 cut(s) 68, 105, 294, 323, 429
FauNDI CATATG 1 cut(s) 502
FblI GTMKAC 2 cut(s) 570, 603
Fnu4HI GCNGC 1 cut(s) 226
FokI GGATG 5 cut(s) 181, 675, 679, 714, 731
Fsp4HI GCNGC 1 cut(s) 226
FspBI CTAG 1 cut(s) 413
GluI GCNGC 1 cut(s) 226
HaeIII GGCC 4 cut(s) 110, 491, 768, 796
HapII CCGG 1 cut(s) 60
Hin1II CATG 5 cut(s) 72, 109, 298, 327, 433
HinfI GANTC 3 cut(s) 97, 368, 512
HpaII CCGG 1 cut(s) 60
HphI GGTGA 1 cut(s) 441
Hpy166II GTNNAC 3 cut(s) 571, 604, 752
Hpy188I TCNGA 5 cut(s) 148, 367, 398, 526, 588
Hpy188III TCNNGA 5 cut(s) 155, 168, 372, 632, 647
Hpy8I GTNNAC 3 cut(s) 571, 604, 752
Hpy99I CGWCG 1 cut(s) 148
HpyAV CCTTC 1 cut(s) 531
HpyCH4III ACNGT 2 cut(s) 460, 568
HpyCH4IV ACGT 2 cut(s) 610, 640
HpyCH4V TGCA 8 cut(s) 68, 225, 305, 333, 380, 500, 581, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 497
HpyF3I CTNAG 2 cut(s) 395, 525
HpySE526I ACGT 2 cut(s) 610, 640
Hsp92II CATG 5 cut(s) 72, 109, 298, 327, 433
Kzo9I GATC 1 cut(s) 310
LmnI GCTCC 1 cut(s) 684
LpnPI CCDG 9 cut(s) 73, 168, 181, 202, 264, 391, 610, 617, 665
Lsp1109I GCAGC 1 cut(s) 237
LweI GCATC 1 cut(s) 55
MaeI CTAG 1 cut(s) 413
MaeII ACGT 2 cut(s) 610, 640
MaeIII GTNAC 2 cut(s) 175, 290
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 3 cut(s) 601, 635, 647
MfeI CAATTG 1 cut(s) 440
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 2 cut(s) 472, 754
MlsI TGGCCA 1 cut(s) 491
MluCI AATT 6 cut(s) 159, 318, 440, 547, 783, 802
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 1 cut(s) 506
MmeI TCCRAC 3 cut(s) 126, 222, 332
MnlI CCTC 6 cut(s) 4, 88, 348, 373, 376, 772
Mox20I TGGCCA 1 cut(s) 491
Mph1103I ATGCAT 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 642
MscI TGGCCA 1 cut(s) 491
MseI TTAA 2 cut(s) 299, 744
MslI CAYNNNNRTG 1 cut(s) 328
Msp20I TGGCCA 1 cut(s) 491
MspI CCGG 1 cut(s) 60
MspR9I CCNGG 1 cut(s) 60
MunI CAATTG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 386, 497
NciI CCSGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 105
NdeI CATATG 1 cut(s) 502
NdeII GATC 1 cut(s) 310
NlaIII CATG 5 cut(s) 72, 109, 298, 327, 433
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 1 cut(s) 175
NsiI ATGCAT 1 cut(s) 70
NspI RCATGY 2 cut(s) 72, 298
PciI ACATGT 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 86
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 2 cut(s) 97, 368
PflMI CCANNNNNTGG 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 58
PkrI GCNGC 1 cut(s) 227
PleI GAGTC 1 cut(s) 506
PpsI GAGTC 1 cut(s) 506
PscI ACATGT 1 cut(s) 294
PsiI TTATAA 1 cut(s) 711
PspN4I GGNNCC 1 cut(s) 427
PsuI RGATCY 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 328
SaqAI TTAA 2 cut(s) 299, 744
SatI GCNGC 1 cut(s) 226
Sau3AI GATC 1 cut(s) 310
SchI GAGTC 1 cut(s) 506
ScrFI CCNGG 1 cut(s) 60
SduI GDGCHC 2 cut(s) 472, 754
SfaNI GCATC 1 cut(s) 55
SmiMI CAYNNNNRTG 1 cut(s) 328
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
Sse9I AATT 6 cut(s) 159, 318, 440, 547, 783, 802
SspMI CTAG 1 cut(s) 413
StyD4I CCNGG 1 cut(s) 58
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 2 cut(s) 460, 568
TaiI ACGT 2 cut(s) 613, 643
TaqI TCGA 3 cut(s) 33, 95, 240
TasI AATT 6 cut(s) 159, 318, 440, 547, 783, 802
TfiI GAWTC 2 cut(s) 97, 368
Tru1I TTAA 2 cut(s) 299, 744
Tru9I TTAA 2 cut(s) 299, 744
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 225
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 183, 202, 446
TspRI CASTG 1 cut(s) 571
Van91I CCANNNNNTGG 1 cut(s) 408
VneI GTGCAC 1 cut(s) 750
XapI RAATTY 1 cut(s) 159
XceI RCATGY 2 cut(s) 72, 298
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 570, 603
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 1 cut(s) 413
Zsp2I ATGCAT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.