pycom05g00480

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008530
Physical Location & Seq
Reverse (-)
605132 .. 607341
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00480.2

Sequence Viewer

Length: 951 bp
ATGCTTGATCCCAACACAAACCCACATGAACACCCATTCCTCTTCAACAGCCGCGCCGACGACCACCAAATTAAAAGTGACACAACAACAAACTACGATTCTTGGCATTCCGTAAATTACGCTTCCCGGGCTCCTCAGAAAACTACTCAAAAATATGGAAACCACAGCATGGGAGGCCGGATCAACGTCGATACTACTACTGCTTCCGGTGACGAGTCTGACTCGCCGCCGTTGCGGAAGACGACGAGCCGCGTGAAGAACTATCGGAGTCTGTCCCCGACTTCGCAAAAACAAGCGATCGCCAGAGGACAGACGGAGCTCATGGAGATGGTGAAGACCATGCCTGAGTCGTGCTACGAGCTTTCGCTGAAAGATCTCGTTGAGCATAAGTATAGGCCAATGGGTGGCGACGAAGAAGAAGATCGAAATCAATGTCGAGGTCAAGAGGAAGAATACGATGATAGAAGTACTGAGAATTTGGTAAGGAAGGGAAATGGAGACGTAAACGAAAACGAAATGAAGAAAAATGGTGAGAGGAAGAAACTGGTGATGAGGAGTGGGAGCATAGATAGTGGGGGGTTTCTTTTGAAGATGGTTTTGCCAATTTCTTTGGGGGGTAAGAAGAAGAATAATAAGAGCGTTAAGGAGAAGGGTGAGTGCTTGGTGGTGGATGCAAATAGCAATAATATGAGTGCAAAGGTGTCTCCAAAGCCTCAGGGGTTGGATGGATCTGTGAAGGTAGTGGGTGTGGATAATGAATGGTGGAAGATGAAGAGATTTTCTGTATCAGAAAGGAGTGAAAGTGGTGTTTCTAGTATCAATAGTGGAAGCATAAAAAGCAGTGGCAGCGGTAGCAGCAGCAGCAGCTGTAGCAGCAGAAGCAACGGCAGGCGCAAGAGCAGTGGATGCTGGTCGTTCATCCTGCTCAAGAAGAACAAGAGGCGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

317

Amino Acids

35.13

Weight (kDa)

9.32

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 169, 404
AccII CGCG 2 cut(s) 54, 252
AciI CCGC 6 cut(s) 52, 227, 235, 250, 849, 943
AclWI GGATC 3 cut(s) 2, 188, 736
AcsI RAATTY 1 cut(s) 475
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 2 cut(s) 169, 404
AgsI TTSAA 2 cut(s) 46, 589
AloI GAACNNNNNNTCC 2 cut(s) 21, 53
AluBI AGCT 3 cut(s) 319, 361, 867
AluI AGCT 3 cut(s) 319, 361, 867
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 492, 708
AlwI GGATC 3 cut(s) 2, 188, 736
AlwNI CAGNNNCTG 1 cut(s) 867
Ama87I CYCGRG 1 cut(s) 126
AoxI GGCC 2 cut(s) 175, 395
ApeKI GCWGC 6 cut(s) 846, 855, 858, 861, 864, 873
ApoI RAATTY 1 cut(s) 475
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
AsiSI GCGATCGC 1 cut(s) 300
AspLEI GCGC 2 cut(s) 56, 894
AsuC2I CCSGG 2 cut(s) 127, 128
AsuHPI GGTGA 5 cut(s) 221, 343, 542, 559, 665
AvaI CYCGRG 1 cut(s) 126
AxyI CCTNAGG 1 cut(s) 714
BanII GRGCYC 2 cut(s) 133, 321
BbsI GAAGAC 2 cut(s) 245, 341
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 6 cut(s) 858, 867, 870, 873, 876, 885
BccI CCATC 3 cut(s) 322, 586, 719
BceAI ACGGC 2 cut(s) 214, 901
BcnI CCSGG 2 cut(s) 127, 128
BcoDI GTCTC 2 cut(s) 492, 708
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 1 cut(s) 868
BglII AGATCT 1 cut(s) 373
BisI GCNGC 9 cut(s) 52, 227, 250, 847, 856, 859, 862, 865, 874
BlsI GCNGC 9 cut(s) 53, 228, 251, 848, 857, 860, 863, 866, 875
BmcAI AGTACT 1 cut(s) 469
Bme1390I CCNGG 2 cut(s) 127, 128
BmeT110I CYCGRG 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 132
BmrFI CCNGG 2 cut(s) 127, 128
BmsI GCATC 2 cut(s) 661, 896
BpiI GAAGAC 2 cut(s) 245, 341
BplI GAGNNNNNCTC 2 cut(s) 206, 238
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 2 cut(s) 127, 128
BsaBI GATNNNNATC 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 126
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 2 cut(s) 787, 817
Bsc4I CCNNNNNNNGG 2 cut(s) 169, 404
Bse1I ACTGG 1 cut(s) 549
Bse21I CCTNAGG 1 cut(s) 714
Bse8I GATNNNNATC 1 cut(s) 426
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 4 cut(s) 676, 730, 911, 918
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 169, 404
BseMII CTCAG 4 cut(s) 149, 336, 462, 728
BseNI ACTGG 1 cut(s) 549
BseRI GAGGAG 2 cut(s) 123, 568
BseXI GCAGC 6 cut(s) 858, 867, 870, 873, 876, 885
Bsh1236I CGCG 2 cut(s) 54, 252
Bsh1285I CGRYCG 1 cut(s) 300
BshFI GGCC 2 cut(s) 177, 397
BsiEI CGRYCG 1 cut(s) 300
BsiHKAI GWGCWC 1 cut(s) 321
BsiHKCI CYCGRG 1 cut(s) 126
BsiSI CCGG 3 cut(s) 127, 178, 207
BslFI GGGAC 1 cut(s) 259
BslI CCNNNNNNNGG 2 cut(s) 169, 404
BsmAI GTCTC 2 cut(s) 492, 708
BsmBI CGTCTC 1 cut(s) 492
BsmFI GGGAC 1 cut(s) 259
BsmI GAATGC 1 cut(s) 106
BsnI GGCC 2 cut(s) 177, 397
BsoBI CYCGRG 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 133, 321
Bsp143I GATC 6 cut(s) 7, 180, 297, 373, 421, 728
BspACI CCGC 6 cut(s) 52, 227, 235, 250, 849, 943
BspANI GGCC 2 cut(s) 177, 397
BspCNI CTCAG 4 cut(s) 148, 337, 463, 727
BspFNI CGCG 2 cut(s) 54, 252
BspLI GGNNCC 1 cut(s) 132
BspPI GGATC 3 cut(s) 2, 188, 736
BsrI ACTGG 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 6 cut(s) 7, 180, 297, 373, 421, 728
Bst6I CTCTTC 2 cut(s) 47, 767
BstAPI GCANNNNNTGC 1 cut(s) 906
BstC8I GCNNGC 1 cut(s) 890
BstDEI CTNAG 4 cut(s) 135, 345, 471, 714
BstF5I GGATG 4 cut(s) 676, 730, 911, 918
BstFNI CGCG 2 cut(s) 54, 252
BstHHI GCGC 2 cut(s) 56, 894
BstKTI GATC 6 cut(s) 10, 183, 300, 376, 424, 731
BstMAI GTCTC 2 cut(s) 492, 708
BstMBI GATC 6 cut(s) 7, 180, 297, 373, 421, 728
BstMCI CGRYCG 1 cut(s) 300
BstSCI CCNGG 2 cut(s) 125, 126
BstSFI CTRYAG 1 cut(s) 868
BstUI CGCG 2 cut(s) 54, 252
BstV1I GCAGC 6 cut(s) 858, 867, 870, 873, 876, 885
BstV2I GAAGAC 2 cut(s) 245, 341
BstX2I RGATCY 2 cut(s) 373, 728
BstYI RGATCY 2 cut(s) 373, 728
Bsu36I CCTNAGG 1 cut(s) 714
BsuRI GGCC 2 cut(s) 177, 397
BtsCI GGATG 4 cut(s) 676, 730, 911, 918
BtsI GCAGTG 2 cut(s) 847, 907
BtsIMutI CAGTG 2 cut(s) 847, 907
Cac8I GCNNGC 1 cut(s) 890
CaiI CAGNNNCTG 1 cut(s) 867
CfoI GCGC 2 cut(s) 56, 894
Cfr9I CCCGGG 1 cut(s) 126
Csp6I GTAC 1 cut(s) 468
CspCI CAANNNNNGTGG 2 cut(s) 883, 918
CviAII CATG 4 cut(s) 26, 169, 322, 340
CviJI RGCY 9 cut(s) 51, 131, 177, 249, 319, 361, 397, 712, 867
CviKI_1 RGCY 9 cut(s) 51, 131, 177, 249, 319, 361, 397, 712, 867
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 4 cut(s) 135, 345, 471, 714
DpnI GATC 6 cut(s) 9, 182, 299, 375, 423, 730
DpnII GATC 6 cut(s) 7, 180, 297, 373, 421, 728
Eam1104I CTCTTC 2 cut(s) 47, 767
EarI CTCTTC 2 cut(s) 47, 767
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 2 cut(s) 133, 321
Eco53kI GAGCTC 1 cut(s) 319
Eco81I CCTNAGG 1 cut(s) 714
Eco88I CYCGRG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 319
EcoT38I GRGCYC 2 cut(s) 133, 321
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 4 cut(s) 29, 172, 325, 343
FaqI GGGAC 1 cut(s) 259
FatI CATG 4 cut(s) 25, 168, 321, 339
FauI CCCGC 1 cut(s) 936
Fnu4HI GCNGC 9 cut(s) 52, 227, 250, 847, 856, 859, 862, 865, 874
FokI GGATG 4 cut(s) 683, 737, 905, 918
FriOI GRGCYC 2 cut(s) 133, 321
Fsp4HI GCNGC 9 cut(s) 52, 227, 250, 847, 856, 859, 862, 865, 874
FspBI CTAG 1 cut(s) 813
GlaI GCGC 2 cut(s) 55, 893
GluI GCNGC 9 cut(s) 52, 227, 250, 847, 856, 859, 862, 865, 874
HaeIII GGCC 2 cut(s) 177, 397
HapII CCGG 3 cut(s) 127, 178, 207
HhaI GCGC 2 cut(s) 56, 894
Hin1II CATG 4 cut(s) 29, 172, 325, 343
Hin6I GCGC 2 cut(s) 54, 892
HinP1I GCGC 2 cut(s) 54, 892
HinfI GANTC 5 cut(s) 98, 215, 221, 268, 347
HpaII CCGG 3 cut(s) 127, 178, 207
HphI GGTGA 5 cut(s) 221, 343, 542, 559, 665
Hpy166II GTNNAC 1 cut(s) 505
Hpy188I TCNGA 4 cut(s) 138, 220, 267, 790
Hpy188III TCNNGA 2 cut(s) 443, 928
Hpy8I GTNNAC 1 cut(s) 505
Hpy99I CGWCG 4 cut(s) 62, 191, 247, 413
HpyAV CCTTC 3 cut(s) 481, 643, 730
HpyCH4IV ACGT 2 cut(s) 186, 501
HpyCH4V TGCA 2 cut(s) 674, 695
HpyF3I CTNAG 4 cut(s) 135, 345, 471, 714
HpySE526I ACGT 2 cut(s) 186, 501
Hsp92II CATG 4 cut(s) 29, 172, 325, 343
HspAI GCGC 2 cut(s) 54, 892
Kzo9I GATC 6 cut(s) 7, 180, 297, 373, 421, 728
LmnI GCTCC 3 cut(s) 136, 316, 561
Lsp1109I GCAGC 6 cut(s) 858, 867, 870, 873, 876, 885
LweI GCATC 2 cut(s) 661, 896
MaeI CTAG 1 cut(s) 813
MaeII ACGT 2 cut(s) 186, 501
MaeIII GTNAC 2 cut(s) 77, 209
MalI GATC 6 cut(s) 9, 182, 299, 375, 423, 730
MboI GATC 6 cut(s) 7, 180, 297, 373, 421, 728
MflI RGATCY 2 cut(s) 373, 728
MhlI GDGCHC 2 cut(s) 133, 321
MluCI AATT 4 cut(s) 69, 115, 475, 603
MlyI GAGTC 4 cut(s) 215, 224, 277, 356
MmeI TCCRAC 1 cut(s) 702
MseI TTAA 2 cut(s) 72, 642
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 2 cut(s) 849, 867
MspI CCGG 3 cut(s) 127, 178, 207
MspR9I CCNGG 2 cut(s) 127, 128
Mva1269I GAATGC 1 cut(s) 106
MvnI CGCG 2 cut(s) 54, 252
NciI CCSGG 2 cut(s) 127, 128
NdeII GATC 6 cut(s) 7, 180, 297, 373, 421, 728
NlaIII CATG 4 cut(s) 29, 172, 325, 343
NlaIV GGNNCC 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 77, 209
PctI GAATGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 98
PflMI CCANNNNNTGG 2 cut(s) 169, 404
PkrI GCNGC 9 cut(s) 53, 228, 251, 848, 857, 860, 863, 866, 875
Ple19I CGATCG 1 cut(s) 300
PleI GAGTC 4 cut(s) 215, 223, 276, 355
PpsI GAGTC 4 cut(s) 215, 223, 276, 355
Psp124BI GAGCTC 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 132
PstNI CAGNNNCTG 1 cut(s) 867
PsuI RGATCY 2 cut(s) 373, 728
PvuI CGATCG 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 867
RgaI GCGATCGC 1 cut(s) 300
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 321
SaqAI TTAA 2 cut(s) 72, 642
SatI GCNGC 9 cut(s) 52, 227, 250, 847, 856, 859, 862, 865, 874
Sau3AI GATC 6 cut(s) 7, 180, 297, 373, 421, 728
ScaI AGTACT 1 cut(s) 469
SchI GAGTC 4 cut(s) 215, 224, 277, 356
ScrFI CCNGG 2 cut(s) 127, 128
SduI GDGCHC 2 cut(s) 133, 321
SetI ASST 8 cut(s) 189, 321, 363, 442, 504, 702, 741, 869
SfaAI GCGATCGC 1 cut(s) 300
SfaNI GCATC 2 cut(s) 661, 896
SfcI CTRYAG 1 cut(s) 868
SgfI GCGATCGC 1 cut(s) 300
SmaI CCCGGG 1 cut(s) 128
SmiMI CAYNNNNRTG 1 cut(s) 326
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
Sse9I AATT 4 cut(s) 69, 115, 475, 603
SsiI CCGC 6 cut(s) 52, 227, 235, 250, 849, 943
SspMI CTAG 1 cut(s) 813
SstI GAGCTC 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 125, 126
TaiI ACGT 2 cut(s) 189, 504
TaqI TCGA 3 cut(s) 189, 424, 436
TasI AATT 4 cut(s) 69, 115, 475, 603
TatI WGTACW 1 cut(s) 467
TauI GCSGC 3 cut(s) 54, 229, 252
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 2 cut(s) 72, 642
Tru9I TTAA 2 cut(s) 72, 642
TscAI CASTG 2 cut(s) 847, 907
TseFI GTSAC 2 cut(s) 77, 209
TseI GCWGC 6 cut(s) 846, 855, 858, 861, 864, 873
Tsp45I GTSAC 2 cut(s) 77, 209
TspDTI ATGAA 5 cut(s) 42, 533, 771, 785, 907
TspGWI ACGGA 2 cut(s) 100, 329
TspMI CCCGGG 1 cut(s) 126
TspRI CASTG 2 cut(s) 847, 907
Van91I CCANNNNNTGG 2 cut(s) 169, 404
XapI RAATTY 1 cut(s) 475
XmaI CCCGGG 1 cut(s) 126
XspI CTAG 1 cut(s) 813
ZrmI AGTACT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.