Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0002403

Physical Location & Seq

Genomic Coordinates Forward (+)

4371072 .. 4373260

Gene Structure 2189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g03830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1080 bp

ATGGCACCTACAGTGACTTCAGTGGACGCATCTGAACCCAAGGTAGCAAGCATCAAAACCGTGGCCGACTCAGATGCTCTCACTTATGTACCTTCCGAGTACACCTACACCATGGATCCCAACGACAAGGGAGATGAAAACGACCCAGAACACTCAATCCCCATCATTGATTTTGCTCTTCTCACCTCTGCCTCCCCTGATGAACGGGCACAAATGATCCAAAAAATAGGCAAAGCTTGCGAAGAATGGGGCTTCTTTCAGGTGATTAATCACGGTGTACCCGAAAGCCTGATGAAAGAAATGATCGACGCGTGTCGAAGATTTTTTGAGCTGCCGGAGGAGGAGAAGAAGGAGTTCCAAACAAGGAACCTGCTGGACCCTATTAAGTGCGGCACCAGTTTCAACGTCGCAATTGACAAAGTTCGTCTCTGGAGGGATTATCTCAAGGTCATAGCACACCCTGAGTTCAACTCACTCTACAAACCTGCTGGATACAGCGAGGTGTCATTGGAGTTCAGTAAAAGAACCCATGCAGTGGCAACCGAAATTTTGAATGGAATATCGGAGAGCTTGGGATTGGAGGCCGATTACATTGCGAAGGCCATGAACTGGGATCGCGGCTTGCAAATTCTGGCCGCGAACTACTACCCGGCTTGCCCACAGCCCGACCTGGCTATCGGTATTCCTCCTCATACCGATCACGGACTGGTGACACTCTTGATTCAGAATGATATGTGTGGCCTCGAAGTCAAGCACAATGATCAATGGGTCTTGGTGAAAGCTGCTCCCGGCGCTTTTGTTGTTAACGTTGGCGATCAAATGCAGATTCTGACAAACGACAAGTACAAGAGCGTATGGCATCGAGCAACTGTGAACAACACAGCTACGAGGATATCGATCGCAGTACCACATGGACCGGCACTGGACACACCTGCTCTACCGATCTCGGAGTTGCTAGAAAAGGAAGGCGAAAAAGCAAAGTACATTGGAATGACATATGAGAAATTCATGGAACTTCAGGCAAGCCCCGCTGCCTACATGATGCCTTGCTTGGATCACCTGCGCGTCAAGGACAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

360

Amino Acids

40.06

Weight (kDa)

5.02

Isoelectric Point (pI)

35.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	53 - 159	1.1e-28	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	209 - 304	6.7e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002403)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g38040 FvH4_5g38040 FvH4_5g38050
malus_domestica	MD05G1046000.v1.1 MD10G1052800.v1.1 MD10G1053000.v1.1 MD10G1053100.v1.1 MD10G1053200.v1.1
prunus_persica	Prupe.8G066500_v2.0.a1 Prupe.8G066600_v2.0.a1 Prupe.8G066600_v2.0.a1
pyrus_communis	pycom05g03830 pycom10g03780 pycom10g03830 pycom10g03910 pycom10g04020
rosa_chinensis	RchiOBHm_Chr6g0265671 RchiOBHm_Chr7g0239961 RchiOBHm_Chr7g0239991
rosa_laevigata	RLG00000000713 RLG00000000714 RLG00000014168
rosa_multiflora	Rmu_co8141760.1_g000001 Rmu_sc0000540.1_g000014 Rmu_sc0000540.1_g000017 Rmu_sc0006825.1_g000004
rosa_roxburghii	Rroxscaffold_3G00220550 Rroxscaffold_3G00220590
rosa_rugosa	Rorug06G0018400 Rorug07G0331500
rosa_samantha	Rh6BG140400 Rh6CG137300 Rh6DG123800 Rh7AG485400 Rh7AG485500 Rh7BG458200 Rh7BG458300 Rh7CG502500 Rh7CG502700 Rh7DG470900 Rh7DG471000
rosa_wichuraiana	Rw7G040700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	940, 1068
Acc36I	ACCTGC	4 cut(s)	378, 493, 940, 1068
AccB1I	GGYRCC	2 cut(s)	4, 392
AccB7I	CCANNNNNTGG	2 cut(s)	535, 609
AccII	CGCG	4 cut(s)	311, 618, 638, 1065
AciI	CCGC	4 cut(s)	390, 618, 636, 1029
AclI	AACGTT	1 cut(s)	807
AclWI	GGATC	5 cut(s)	110, 123, 211, 621, 1062
AcoI	YGGCCR	2 cut(s)	63, 633
AcsI	RAATTY	3 cut(s)	546, 627, 1004
AcuI	CTGAAG	1 cut(s)	1001
AfaI	GTAC	6 cut(s)	90, 101, 279, 845, 906, 983
AfiI	CCNNNNNNNGG	2 cut(s)	535, 609
AflIII	ACRYGT	1 cut(s)	309
AgsI	TTSAA	3 cut(s)	403, 469, 553
AjnI	CCWGG	1 cut(s)	669
AloI	GAACNNNNNNTCC	2 cut(s)	141, 173
AluBI	AGCT	5 cut(s)	236, 331, 570, 782, 884
AluI	AGCT	5 cut(s)	236, 331, 570, 782, 884
Alw26I	GTCTC	1 cut(s)	431
AlwI	GGATC	5 cut(s)	110, 123, 211, 621, 1062
AlwNI	CAGNNNCTG	1 cut(s)	829
AoxI	GGCC	5 cut(s)	63, 582, 600, 633, 739
ApeKI	GCWGC	3 cut(s)	331, 782, 1031
ApoI	RAATTY	3 cut(s)	546, 627, 1004
AseI	ATTAAT	1 cut(s)	267
Asp700I	GAANNNNTTC	1 cut(s)	353
AspLEI	GCGC	2 cut(s)	794, 1065
AspS9I	GGNCC	2 cut(s)	376, 914
AsuC2I	CCSGG	2 cut(s)	650, 789
AsuHPI	GGTGA	5 cut(s)	175, 274, 721, 787, 1049
AvaII	GGWCC	2 cut(s)	376, 914
BaeGI	GKGCMC	1 cut(s)	211
BaeI	ACNNNNGTAYC	2 cut(s)	72, 105
BamHI	GGATCC	1 cut(s)	115
BanI	GGYRCC	2 cut(s)	4, 392
BbvI	GCAGC	3 cut(s)	318, 769, 1018
BccI	CCATC	1 cut(s)	170
BcgI	CGANNNNNNTGC	4 cut(s)	56, 90, 575, 609
BciT130I	CCWGG	1 cut(s)	671
BciVI	GTATCC	1 cut(s)	485
BclI	TGATCA	1 cut(s)	760
BcnI	CCSGG	2 cut(s)	650, 789
BcoDI	GTCTC	1 cut(s)	431
BfaI	CTAG	1 cut(s)	956
BfmI	CTRYAG	1 cut(s)	9
BfoI	RGCGCY	1 cut(s)	795
BfuAI	ACCTGC	4 cut(s)	378, 493, 940, 1068
BfuI	GTATCC	1 cut(s)	485
BisI	GCNGC	6 cut(s)	332, 391, 619, 636, 783, 1032
BlsI	GCNGC	6 cut(s)	333, 392, 620, 637, 784, 1033
Bme1390I	CCNGG	3 cut(s)	650, 671, 789
Bme18I	GGWCC	2 cut(s)	376, 914
BmgT120I	GGNCC	2 cut(s)	376, 914
BmiI	GGNNCC	5 cut(s)	6, 117, 368, 378, 394
BmrFI	CCNGG	3 cut(s)	650, 671, 789
BmrI	ACTGGG	1 cut(s)	619
BmsI	GCATC	5 cut(s)	38, 60, 64, 868, 1032
BmuI	ACTGGG	1 cut(s)	619
BoxI	GACNNNNGTC	1 cut(s)	312
BplI	GAGNNNNNCTC	2 cut(s)	455, 487
BpmI	CTGGAG	1 cut(s)	451
BpuEI	CTTGAG	1 cut(s)	428
BpuMI	CCSGG	2 cut(s)	650, 789
Bsa29I	ATCGAT	1 cut(s)	896
BsaBI	GATNNNNATC	1 cut(s)	896
BsaJI	CCNNGG	3 cut(s)	39, 60, 111
Bsc4I	CCNNNNNNNGG	2 cut(s)	535, 609
Bse118I	RCCGGY	1 cut(s)	916
Bse1I	ACTGG	4 cut(s)	396, 614, 711, 927
Bse3DI	GCAATG	1 cut(s)	591
Bse8I	GATNNNNATC	1 cut(s)	896
BseBI	CCWGG	1 cut(s)	671
BseCI	ATCGAT	1 cut(s)	896
BseDI	CCNNGG	3 cut(s)	39, 60, 111
BseJI	GATNNNNATC	1 cut(s)	896
BseLI	CCNNNNNNNGG	2 cut(s)	535, 609
BseMI	GCAATG	1 cut(s)	591
BseMII	CTCAG	2 cut(s)	84, 453
BseNI	ACTGG	4 cut(s)	396, 614, 711, 927
BseRI	GAGGAG	3 cut(s)	353, 356, 678
BseSI	GKGCMC	1 cut(s)	211
BseXI	GCAGC	3 cut(s)	318, 769, 1018
Bsh1236I	CGCG	4 cut(s)	311, 618, 638, 1065
Bsh1285I	CGRYCG	1 cut(s)	900
BshFI	GGCC	5 cut(s)	65, 584, 602, 635, 741
BshNI	GGYRCC	2 cut(s)	4, 392
BshVI	ATCGAT	1 cut(s)	896
BsiEI	CGRYCG	1 cut(s)	900
BsiSI	CCGG	4 cut(s)	335, 650, 789, 917
BslI	CCNNNNNNNGG	2 cut(s)	535, 609
BsmAI	GTCTC	1 cut(s)	431
BsmBI	CGTCTC	1 cut(s)	431
BsnI	GGCC	5 cut(s)	65, 584, 602, 635, 741
Bsp1286I	GDGCHC	1 cut(s)	211
Bsp19I	CCATGG	1 cut(s)	111
BspACI	CCGC	4 cut(s)	390, 618, 636, 1029
BspANI	GGCC	5 cut(s)	65, 584, 602, 635, 741
BspCNI	CTCAG	2 cut(s)	83, 454
BspDI	ATCGAT	1 cut(s)	896
BspFNI	CGCG	4 cut(s)	311, 618, 638, 1065
BspLI	GGNNCC	5 cut(s)	6, 117, 368, 378, 394
BspMI	ACCTGC	4 cut(s)	378, 493, 940, 1068
BspPI	GGATC	5 cut(s)	110, 123, 211, 621, 1062
BspQI	GCTCTTC	1 cut(s)	183
BspT107I	GGYRCC	2 cut(s)	4, 392
BsrDI	GCAATG	1 cut(s)	591
BsrFI	RCCGGY	1 cut(s)	916
BsrI	ACTGG	4 cut(s)	396, 614, 711, 927
BssAI	RCCGGY	1 cut(s)	916
BssECI	CCNNGG	3 cut(s)	39, 60, 111
BssT1I	CCWWGG	2 cut(s)	39, 111
Bst2UI	CCWGG	1 cut(s)	671
Bst4CI	ACNGT	4 cut(s)	13, 61, 275, 871
Bst6I	CTCTTC	1 cut(s)	183
BstAPI	GCANNNNNTGC	1 cut(s)	237
BstC8I	GCNNGC	5 cut(s)	49, 238, 623, 655, 1024
BstDEI	CTNAG	2 cut(s)	70, 462
BstDSI	CCRYGG	2 cut(s)	60, 111
BstFNI	CGCG	4 cut(s)	311, 618, 638, 1065
BstH2I	RGCGCY	1 cut(s)	795
BstHHI	GCGC	2 cut(s)	794, 1065
BstMAI	GTCTC	1 cut(s)	431
BstMCI	CGRYCG	1 cut(s)	900
BstMWI	GCNNNNNNNGC	3 cut(s)	237, 791, 1028
BstNI	CCWGG	1 cut(s)	671
BstPAI	GACNNNNGTC	1 cut(s)	312
BstSCI	CCNGG	3 cut(s)	648, 669, 787
BstSFI	CTRYAG	1 cut(s)	9
BstSLI	GKGCMC	1 cut(s)	211
BstUI	CGCG	4 cut(s)	311, 618, 638, 1065
BstV1I	GCAGC	3 cut(s)	318, 769, 1018
BstX2I	RGATCY	1 cut(s)	115
BstYI	RGATCY	1 cut(s)	115
Bsu15I	ATCGAT	1 cut(s)	896
BsuI	GTATCC	1 cut(s)	485
BsuRI	GGCC	5 cut(s)	65, 584, 602, 635, 741
BsuTUI	ATCGAT	1 cut(s)	896
BtgI	CCRYGG	2 cut(s)	60, 111
BtsI	GCAGTG	1 cut(s)	540
BtsIMutI	CAGTG	4 cut(s)	18, 27, 540, 920
BveI	ACCTGC	4 cut(s)	378, 493, 940, 1068
Cac8I	GCNNGC	5 cut(s)	49, 238, 623, 655, 1024
CaiI	CAGNNNCTG	1 cut(s)	829
CfoI	GCGC	2 cut(s)	794, 1065
Cfr10I	RCCGGY	1 cut(s)	916
Cfr13I	GGNCC	2 cut(s)	376, 914
ClaI	ATCGAT	1 cut(s)	896
CseI	GACGC	3 cut(s)	35, 317, 1054
Csp6I	GTAC	6 cut(s)	89, 100, 278, 844, 905, 982
CviAII	CATG	6 cut(s)	112, 530, 604, 911, 1009, 1039
CviQI	GTAC	6 cut(s)	89, 100, 278, 844, 905, 982
DdeI	CTNAG	2 cut(s)	70, 462
EaeI	YGGCCR	2 cut(s)	63, 633
Eam1104I	CTCTTC	1 cut(s)	183
EarI	CTCTTC	1 cut(s)	183
Eco130I	CCWWGG	2 cut(s)	39, 111
Eco32I	GATATC	1 cut(s)	894
Eco47I	GGWCC	2 cut(s)	376, 914
Eco57I	CTGAAG	1 cut(s)	1001
EcoRII	CCWGG	1 cut(s)	669
EcoRV	GATATC	1 cut(s)	894
EcoT14I	CCWWGG	2 cut(s)	39, 111
ErhI	CCWWGG	2 cut(s)	39, 111
Esp3I	CGTCTC	1 cut(s)	431
FaeI	CATG	6 cut(s)	115, 533, 607, 914, 1012, 1042
FatI	CATG	6 cut(s)	111, 529, 603, 910, 1008, 1038
FauI	CCCGC	1 cut(s)	1036
FauNDI	CATATG	1 cut(s)	997
FbaI	TGATCA	1 cut(s)	760
Fnu4HI	GCNGC	6 cut(s)	332, 391, 619, 636, 783, 1032
Fsp4HI	GCNGC	6 cut(s)	332, 391, 619, 636, 783, 1032
FspBI	CTAG	1 cut(s)	956
GlaI	GCGC	2 cut(s)	793, 1064
GluI	GCNGC	6 cut(s)	332, 391, 619, 636, 783, 1032
GsuI	CTGGAG	1 cut(s)	451
HaeII	RGCGCY	1 cut(s)	795
HaeIII	GGCC	5 cut(s)	65, 584, 602, 635, 741
HapII	CCGG	4 cut(s)	335, 650, 789, 917
HgaI	GACGC	3 cut(s)	35, 317, 1054
HhaI	GCGC	2 cut(s)	794, 1065
Hin1II	CATG	6 cut(s)	115, 533, 607, 914, 1012, 1042
Hin6I	GCGC	2 cut(s)	792, 1063
HinP1I	GCGC	2 cut(s)	792, 1063
HincII	GTYRAC	1 cut(s)	805
HindII	GTYRAC	1 cut(s)	805
HindIII	AAGCTT	1 cut(s)	234
HinfI	GANTC	3 cut(s)	68, 721, 826
HpaI	GTTAAC	1 cut(s)	805
HpaII	CCGG	4 cut(s)	335, 650, 789, 917
HphI	GGTGA	5 cut(s)	175, 274, 721, 787, 1049
Hpy166II	GTNNAC	5 cut(s)	25, 102, 278, 805, 874
Hpy188I	TCNGA	7 cut(s)	34, 73, 97, 565, 726, 831, 949
Hpy188III	TCNNGA	2 cut(s)	430, 718
Hpy8I	GTNNAC	5 cut(s)	25, 102, 278, 805, 874
Hpy99I	CGWCG	2 cut(s)	311, 410
HpyAV	CCTTC	4 cut(s)	102, 343, 592, 959
HpyCH4III	ACNGT	4 cut(s)	13, 61, 275, 871
HpyCH4IV	ACGT	2 cut(s)	405, 807
HpyCH4V	TGCA	3 cut(s)	533, 625, 823
HpyF10VI	GCNNNNNNNGC	3 cut(s)	237, 791, 1028
HpyF3I	CTNAG	2 cut(s)	70, 462
HpySE526I	ACGT	2 cut(s)	405, 807
Hsp92II	CATG	6 cut(s)	115, 533, 607, 914, 1012, 1042
HspAI	GCGC	2 cut(s)	792, 1063
Ksp22I	TGATCA	1 cut(s)	760
KspAI	GTTAAC	1 cut(s)	805
LguI	GCTCTTC	1 cut(s)	183
LmnI	GCTCC	1 cut(s)	790
Lsp1109I	GCAGC	3 cut(s)	318, 769, 1018
LweI	GCATC	5 cut(s)	38, 60, 64, 868, 1032
MaeI	CTAG	1 cut(s)	956
MaeII	ACGT	2 cut(s)	405, 807
MaeIII	GTNAC	2 cut(s)	13, 709
MboII	GAAGA	4 cut(s)	170, 254, 330, 358
MfeI	CAATTG	2 cut(s)	411, 1075
MflI	RGATCY	1 cut(s)	115
MhlI	GDGCHC	1 cut(s)	211
MluCI	AATT	5 cut(s)	411, 546, 627, 1004, 1075
MluI	ACGCGT	1 cut(s)	309
MlyI	GAGTC	1 cut(s)	62
MroXI	GAANNNNTTC	1 cut(s)	353
MseI	TTAA	3 cut(s)	267, 384, 804
MslI	CAYNNNNRTG	1 cut(s)	989
MspA1I	CMGCKG	1 cut(s)	1031
MspI	CCGG	4 cut(s)	335, 650, 789, 917
MspR9I	CCNGG	3 cut(s)	650, 671, 789
MunI	CAATTG	2 cut(s)	411, 1075
MvaI	CCWGG	1 cut(s)	671
MvnI	CGCG	4 cut(s)	311, 618, 638, 1065
MwoI	GCNNNNNNNGC	3 cut(s)	237, 791, 1028
NciI	CCSGG	2 cut(s)	650, 789
NcoI	CCATGG	1 cut(s)	111
NdeI	CATATG	1 cut(s)	997
NlaIII	CATG	6 cut(s)	115, 533, 607, 914, 1012, 1042
NlaIV	GGNNCC	5 cut(s)	6, 117, 368, 378, 394
NmuCI	GTSAC	2 cut(s)	13, 709
PaqCI	CACCTGC	2 cut(s)	940, 1068
PciSI	GCTCTTC	1 cut(s)	183
PcsI	WCGNNNNNNNCGW	2 cut(s)	279, 893
PdmI	GAANNNNTTC	1 cut(s)	353
PfeI	GAWTC	2 cut(s)	721, 826
PflMI	CCANNNNNTGG	2 cut(s)	535, 609
PkrI	GCNGC	6 cut(s)	333, 392, 620, 637, 784, 1033
Ple19I	CGATCG	1 cut(s)	900
PleI	GAGTC	1 cut(s)	62
PpsI	GAGTC	1 cut(s)	62
PshAI	GACNNNNGTC	1 cut(s)	312
PshBI	ATTAAT	1 cut(s)	267
Psp1406I	AACGTT	1 cut(s)	807
Psp6I	CCWGG	1 cut(s)	669
PspGI	CCWGG	1 cut(s)	669
PspN4I	GGNNCC	5 cut(s)	6, 117, 368, 378, 394
PspPI	GGNCC	2 cut(s)	376, 914
PstNI	CAGNNNCTG	1 cut(s)	829
PsuI	RGATCY	1 cut(s)	115
PvuI	CGATCG	1 cut(s)	900
RsaI	GTAC	6 cut(s)	90, 101, 279, 845, 906, 983
RsaNI	GTAC	6 cut(s)	89, 100, 278, 844, 905, 982
RseI	CAYNNNNRTG	1 cut(s)	989
SapI	GCTCTTC	1 cut(s)	183
SaqAI	TTAA	3 cut(s)	267, 384, 804
SatI	GCNGC	6 cut(s)	332, 391, 619, 636, 783, 1032
Sau96I	GGNCC	2 cut(s)	376, 914
SchI	GAGTC	1 cut(s)	62
ScrFI	CCNGG	3 cut(s)	650, 671, 789
SduI	GDGCHC	1 cut(s)	211
SfaNI	GCATC	5 cut(s)	38, 60, 64, 868, 1032
SfcI	CTRYAG	1 cut(s)	9
SinI	GGWCC	2 cut(s)	376, 914
SmiMI	CAYNNNNRTG	1 cut(s)	989
SmlI	CTYRAG	1 cut(s)	443
SmoI	CTYRAG	1 cut(s)	443
Sse9I	AATT	5 cut(s)	411, 546, 627, 1004, 1075
SsiI	CCGC	4 cut(s)	390, 618, 636, 1029
SspMI	CTAG	1 cut(s)	956
StyD4I	CCNGG	3 cut(s)	648, 669, 787
StyI	CCWWGG	2 cut(s)	39, 111
TaaI	ACNGT	4 cut(s)	13, 61, 275, 871
TaiI	ACGT	2 cut(s)	408, 810
TaqI	TCGA	5 cut(s)	306, 316, 744, 862, 896
TasI	AATT	5 cut(s)	411, 546, 627, 1004, 1075
TatI	WGTACW	3 cut(s)	99, 843, 981
TauI	GCSGC	3 cut(s)	393, 621, 638
TfiI	GAWTC	2 cut(s)	721, 826
Tru1I	TTAA	3 cut(s)	267, 384, 804
Tru9I	TTAA	3 cut(s)	267, 384, 804
TscAI	CASTG	4 cut(s)	18, 27, 540, 927
TseFI	GTSAC	2 cut(s)	13, 709
TseI	GCWGC	3 cut(s)	331, 782, 1031
Tsp45I	GTSAC	2 cut(s)	13, 709
TspDTI	ATGAA	5 cut(s)	150, 216, 308, 620, 997
TspGWI	ACGGA	1 cut(s)	717
TspRI	CASTG	4 cut(s)	18, 27, 540, 927
Van91I	CCANNNNNTGG	2 cut(s)	535, 609
VpaK11BI	GGWCC	2 cut(s)	376, 914
VspI	ATTAAT	1 cut(s)	267
XapI	RAATTY	3 cut(s)	546, 627, 1004
XmnI	GAANNNNTTC	1 cut(s)	353
XspI	CTAG	1 cut(s)	956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.