pycom10g03780

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002403
Physical Location & Seq
Forward (+)
4135692 .. 4136666
975 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g03780.1

Sequence Viewer

Length: 708 bp
ATGGCACCTACAGTGACTTCAGTGGATGCCTCCGAGCCCAATGTAGCGACAATCAAAACCTTAGCCGGCTCAGATGCTCTCACTTCTGTACCTTCCGAGTACGCCTACATCATGGATCCCAACGATAAGGGAGATGCAAACGACCCAGAACACTCAATCCCCATCATTGATTTTGCTCTTCTCACCTCTGTCTCCCCAGATGAACGGGCACAAATGATCCAGAAGCTAGGAAAAGCTTGCCAAGAATGGGGTTTCTTTCACAACCACGGTGTACCAGAGAGCTTGATGAAAGAAACGATTGACGTGTGCCGAATATTTTTTGAGCTACCGGAGGAGGAAAAGAAGGAGTTCCATACAAAGAACCTGTTGGACCCAATCAAGTGCGGCACCAGCTTCAACGTCGCGATTGACAAAGTTCGTCTTTGGAGGGATTATCTCAAGGTCATCGCACACCCCGAATTCAACTCACTCTACAAACCTGCTGGATACAGTGAAGTTTCATTGGAGTTCAGCAAAAGAACCCGTGCAGTGGCAAACAAAATATTGAATGGAATATCGGAGAGTTTGGGATTGGAGGCCGATTACATTACCAAGGCCATGAACTGGGATCGATCGGCTGCCAAATTCTGGCAGCGAACTACTACCCGGCTTGCCCACATCCCGACCTGGCAATCGGTATTCCTCCGCATACAGATCATGGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

236

Amino Acids

26.63

Weight (kDa)

5.56

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 53 - 158 1.3e-22 non-haem dioxygenase in morphine synthesis N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 487
AccB1I GGYRCC 2 cut(s) 4, 386
AccB7I CCANNNNNTGG 2 cut(s) 603, 627
AccII CGCG 1 cut(s) 404
AciI CCGC 2 cut(s) 384, 685
AclWI GGATC 4 cut(s) 110, 123, 211, 615
AcsI RAATTY 2 cut(s) 458, 623
AfaI GTAC 3 cut(s) 90, 101, 273
AfiI CCNNNNNNNGG 4 cut(s) 247, 529, 603, 627
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 3 cut(s) 397, 463, 547
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 665
AloI GAACNNNNNNTCC 2 cut(s) 141, 173
AluBI AGCT 5 cut(s) 226, 236, 282, 325, 393
AluI AGCT 5 cut(s) 226, 236, 282, 325, 393
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 4 cut(s) 110, 123, 211, 615
AoxI GGCC 2 cut(s) 576, 594
ApeKI GCWGC 2 cut(s) 617, 631
ApoI RAATTY 2 cut(s) 458, 623
Asp700I GAANNNNTTC 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 370
BaeGI GKGCMC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 72, 105
BamHI GGATCC 1 cut(s) 115
BanI GGYRCC 2 cut(s) 4, 386
BanII GRGCYC 1 cut(s) 39
BbvI GCAGC 2 cut(s) 604, 643
BccI CCATC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 667
BciVI GTATCC 1 cut(s) 479
BcnI CCSGG 1 cut(s) 646
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 1 cut(s) 227
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 1 cut(s) 479
BisI GCNGC 3 cut(s) 385, 618, 632
BlsI GCNGC 3 cut(s) 386, 619, 633
Bme1390I CCNGG 2 cut(s) 646, 667
Bme18I GGWCC 1 cut(s) 370
BmgBI CACGTC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 4 cut(s) 6, 117, 372, 388
BmrFI CCNGG 2 cut(s) 646, 667
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 3 cut(s) 16, 64, 124
BmuI ACTGGG 1 cut(s) 613
Bpu10I CCTNAGC 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 646
Bsa29I ATCGAT 1 cut(s) 610
BsaJI CCNNGG 2 cut(s) 265, 591
BsaWI WCCGGW 1 cut(s) 328
Bsc4I CCNNNNNNNGG 4 cut(s) 247, 529, 603, 627
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 608, 707
BseBI CCWGG 1 cut(s) 667
BseCI ATCGAT 1 cut(s) 610
BseDI CCNNGG 2 cut(s) 265, 591
BseGI GGATG 2 cut(s) 31, 657
BseLI CCNNNNNNNGG 4 cut(s) 247, 529, 603, 627
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 2 cut(s) 608, 707
BseRI GAGGAG 1 cut(s) 347
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 2 cut(s) 604, 643
BsgI GTGCAG 1 cut(s) 546
Bsh1236I CGCG 1 cut(s) 404
Bsh1285I CGRYCG 1 cut(s) 614
BshFI GGCC 2 cut(s) 578, 596
BshNI GGYRCC 2 cut(s) 4, 386
BshVI ATCGAT 1 cut(s) 610
BsiEI CGRYCG 1 cut(s) 614
BsiSI CCGG 3 cut(s) 66, 329, 646
BslI CCNNNNNNNGG 4 cut(s) 247, 529, 603, 627
BsmAI GTCTC 1 cut(s) 196
BsnI GGCC 2 cut(s) 578, 596
Bsp1286I GDGCHC 2 cut(s) 39, 211
Bsp143I GATC 5 cut(s) 115, 216, 607, 611, 693
Bsp68I TCGCGA 1 cut(s) 404
BspACI CCGC 2 cut(s) 384, 685
BspANI GGCC 2 cut(s) 578, 596
BspCNI CTCAG 1 cut(s) 83
BspDI ATCGAT 1 cut(s) 610
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 4 cut(s) 6, 117, 372, 388
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 4 cut(s) 110, 123, 211, 615
BspQI GCTCTTC 1 cut(s) 183
BspT107I GGYRCC 2 cut(s) 4, 386
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 2 cut(s) 608, 707
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 265, 591
BssMI GATC 5 cut(s) 115, 216, 607, 611, 693
BssT1I CCWWGG 1 cut(s) 591
Bst2UI CCWGG 1 cut(s) 667
Bst4CI ACNGT 3 cut(s) 13, 269, 491
Bst6I CTCTTC 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 67, 238, 651
BstDEI CTNAG 2 cut(s) 61, 70
BstDSI CCRYGG 1 cut(s) 265
BstF5I GGATG 2 cut(s) 31, 657
BstFNI CGCG 1 cut(s) 404
BstKTI GATC 5 cut(s) 118, 219, 610, 614, 696
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 5 cut(s) 115, 216, 607, 611, 693
BstMCI CGRYCG 1 cut(s) 614
BstMWI GCNNNNNNNGC 1 cut(s) 390
BstNI CCWGG 1 cut(s) 667
BstSCI CCNGG 2 cut(s) 644, 665
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 604, 643
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 610
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 2 cut(s) 578, 596
BsuTUI ATCGAT 1 cut(s) 610
BtgI CCRYGG 1 cut(s) 265
BtgZI GCGATG 1 cut(s) 430
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 2 cut(s) 31, 657
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 4 cut(s) 18, 27, 496, 534
BtuMI TCGCGA 1 cut(s) 404
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 3 cut(s) 67, 238, 651
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 370
ClaI ATCGAT 1 cut(s) 610
Csp6I GTAC 3 cut(s) 89, 100, 272
CviAII CATG 3 cut(s) 112, 598, 697
CviQI GTAC 3 cut(s) 89, 100, 272
DdeI CTNAG 2 cut(s) 61, 70
DpnI GATC 5 cut(s) 117, 218, 609, 613, 695
DpnII GATC 5 cut(s) 115, 216, 607, 611, 693
Eam1104I CTCTTC 1 cut(s) 183
EarI CTCTTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 591
Eco24I GRGCYC 1 cut(s) 39
Eco47I GGWCC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 665
EcoT14I CCWWGG 1 cut(s) 591
EcoT38I GRGCYC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 3 cut(s) 115, 601, 700
FaiI YATR 5 cut(s) 113, 354, 599, 689, 698
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 3 cut(s) 111, 597, 696
Fnu4HI GCNGC 3 cut(s) 385, 618, 632
FokI GGATG 2 cut(s) 38, 644
FriOI GRGCYC 1 cut(s) 39
Fsp4HI GCNGC 3 cut(s) 385, 618, 632
FspBI CTAG 1 cut(s) 227
GluI GCNGC 3 cut(s) 385, 618, 632
HaeIII GGCC 2 cut(s) 578, 596
HapII CCGG 3 cut(s) 66, 329, 646
Hin1II CATG 3 cut(s) 115, 601, 700
HindIII AAGCTT 1 cut(s) 234
HpaII CCGG 3 cut(s) 66, 329, 646
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 1 cut(s) 272
Hpy188I TCNGA 4 cut(s) 34, 73, 97, 559
Hpy188III TCNNGA 3 cut(s) 220, 403, 661
Hpy8I GTNNAC 1 cut(s) 272
Hpy99I CGWCG 1 cut(s) 404
HpyAV CCTTC 2 cut(s) 102, 337
HpyCH4III ACNGT 3 cut(s) 13, 269, 491
HpyCH4IV ACGT 2 cut(s) 303, 399
HpyCH4V TGCA 2 cut(s) 137, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 390
HpyF3I CTNAG 2 cut(s) 61, 70
HpySE526I ACGT 2 cut(s) 303, 399
Hsp92II CATG 3 cut(s) 115, 601, 700
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 5 cut(s) 115, 216, 607, 611, 693
LguI GCTCTTC 1 cut(s) 183
Lsp1109I GCAGC 2 cut(s) 604, 643
LweI GCATC 3 cut(s) 16, 64, 124
MaeI CTAG 1 cut(s) 227
MaeII ACGT 2 cut(s) 303, 399
MaeIII GTNAC 1 cut(s) 13
MalI GATC 5 cut(s) 117, 218, 609, 613, 695
MboI GATC 5 cut(s) 115, 216, 607, 611, 693
MboII GAAGA 1 cut(s) 170
MflI RGATCY 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 39, 211
MluCI AATT 2 cut(s) 458, 623
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 7 cut(s) 40, 196, 325, 328, 420, 568, 692
MroNI GCCGGC 1 cut(s) 65
MroXI GAANNNNTTC 1 cut(s) 347
MspI CCGG 3 cut(s) 66, 329, 646
MspR9I CCNGG 2 cut(s) 646, 667
MvaI CCWGG 1 cut(s) 667
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 1 cut(s) 390
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 646
NdeII GATC 5 cut(s) 115, 216, 607, 611, 693
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 3 cut(s) 115, 601, 700
NlaIV GGNNCC 4 cut(s) 6, 117, 372, 388
NmuCI GTSAC 1 cut(s) 13
NruI TCGCGA 1 cut(s) 404
PciSI GCTCTTC 1 cut(s) 183
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PdiI GCCGGC 1 cut(s) 67
PdmI GAANNNNTTC 1 cut(s) 347
PflMI CCANNNNNTGG 2 cut(s) 603, 627
PkrI GCNGC 3 cut(s) 386, 619, 633
Ple19I CGATCG 1 cut(s) 614
Psp6I CCWGG 1 cut(s) 665
PspGI CCWGG 1 cut(s) 665
PspN4I GGNNCC 4 cut(s) 6, 117, 372, 388
PspPI GGNCC 1 cut(s) 370
PsuI RGATCY 1 cut(s) 115
PvuI CGATCG 1 cut(s) 614
RruI TCGCGA 1 cut(s) 404
RsaI GTAC 3 cut(s) 90, 101, 273
RsaNI GTAC 3 cut(s) 89, 100, 272
SapI GCTCTTC 1 cut(s) 183
SatI GCNGC 3 cut(s) 385, 618, 632
Sau3AI GATC 5 cut(s) 115, 216, 607, 611, 693
Sau96I GGNCC 1 cut(s) 370
ScrFI CCNGG 2 cut(s) 646, 667
SduI GDGCHC 2 cut(s) 39, 211
SfaNI GCATC 3 cut(s) 16, 64, 124
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 2 cut(s) 458, 623
SsiI CCGC 2 cut(s) 384, 685
SspI AATATT 2 cut(s) 315, 543
SspMI CTAG 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 644, 665
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 3 cut(s) 13, 269, 491
TaiI ACGT 2 cut(s) 306, 402
TaqI TCGA 1 cut(s) 610
TasI AATT 2 cut(s) 458, 623
TauI GCSGC 1 cut(s) 387
TscAI CASTG 4 cut(s) 18, 27, 496, 534
TseFI GTSAC 1 cut(s) 13
TseI GCWGC 2 cut(s) 617, 631
Tsp45I GTSAC 1 cut(s) 13
TspDTI ATGAA 4 cut(s) 216, 302, 489, 614
TspRI CASTG 4 cut(s) 18, 27, 496, 534
Van91I CCANNNNNTGG 2 cut(s) 603, 627
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 2 cut(s) 458, 623
XmnI GAANNNNTTC 1 cut(s) 347
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.