pycom05g04160

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010240
Physical Location & Seq
Forward (+)
4895486 .. 4899689
4204 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGGGCAGTAAAAAGAGAAGCTGCACTTCCATGGAAGCTGCGACTGATGCTGTCGCTAACGATGGCGGCTTCAGTAATCTGAAGAAATCGAAGAAGGGCAAAACAAAGCAGGAGACAACAGAAGCTTCTGCCCCTTCTTCTTCTACCGCTCCCACCGCCGTCAAACCCATGGAGAGACAAAAGAAAAGGAAAGCTTTGGACAAAGTGAGGCGCCTCCACACTGAGGAGACCAAACCTAAAGAACCCAAAACAATGGAGGTGCCCGCTTCGTCTTCAACGAGTGGGGTTCTTCCGGAGTTCCATGTGGGTGTGTTTAAGGACTTGGCTTCGGCTGATGGGTCTCTTAGAGAAGCTGCGGCGGAGGCTTTGGCTATGGAGTTGGTGGCCGTGCAGAGAGCTTATGATGGGCTTGAGAATAAGGAGTTGATTGAAGGTGGAGTGAAGTTGGATGCCGAGAAGGATGATGGGTTGAATGATTGTGCGCCTTCCTTGAGATATGCTGTGCGGAGACTCATTCGCGGCGTTTCTTCATCAAGAGAGTGTGCAAGACAAGGGTTTGCATTAGGTTTAACTATTTTAGCAAGTACGATCCCCAGCATCAAGGTGAACTCATTGTTGAAACTTATTGTTGATTTCTTAGAGGTCTCTTCATCGATGAAGGGTCAGTGCAGGAACAAAGGGATCGATCTTTTGGGTCGCTTATTTGCTTATGGTGCTATTGCCCGATCAGGAAGACTAGCTGAGGAGTGGGTTTCTGATAGAAACACTCCTCTCATAAAGGAATTCACTAGTCTTCTCATTGCCCTTGCATCAAAGAAGCGATACTTGCAAGAGCCTGTTGTTTCGGTTATTGTAGACTTGATCGAGAAGTTGCACTCAGAAGCTTTGCTGAATCACATTCTAGAGGCCCCAGGACTTAATCAGTGGTTTGAAGGAGCTATTCAAACTGGAAATCCTGATGCATTGCTTCTGGCTCTGAAAATCAGAGAAAAAACTCTTATTGAAAGTTCAAGTTTTGGGAACCTTTTGCCAGTTCCATTCAGCCCTGACAAACTGTTTTCTGCTGACCATCTGTCTTCTCTTGCTAACTGCTTGAAGGAATCCACCTTTTGTCAGCCTCGTGTTCACAATGTGTGGCCTGTTCTGGTATACATGCTGTTACCTGATAGAGTTTTGCAGGCTGAAGATGCAATTTCTGTTTCAAATTCCTTGAAGAGGCCCAAAAAGAATCGCAAGTCTAGCTCTTCTGATGAAGAAATTGCAAAGAACTTTCAGTGCTTCTGTGAGGTGATCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

433

Amino Acids

47.23

Weight (kDa)

8.84

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 166 - 430 1.3e-47 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 210, 259
AccBSI CCGCTC 1 cut(s) 149
AccI GTMKAC 2 cut(s) 855, 1149
AccII CGCG 1 cut(s) 519
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 8 cut(s) 66, 147, 156, 264, 356, 359, 505, 519
AclWI GGATC 2 cut(s) 583, 689
AcoI YGGCCR 1 cut(s) 384
AcsI RAATTY 2 cut(s) 782, 1204
AcuI CTGAAG 3 cut(s) 55, 101, 1203
AcyI GRCGYC 1 cut(s) 211
AdeI CACNNNGTG 1 cut(s) 1132
AfaI GTAC 1 cut(s) 586
AfiI CCNNNNNNNGG 2 cut(s) 223, 1215
AhdI GACNNNNNGTC 1 cut(s) 1072
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 1 cut(s) 910
Alw26I GTCTC 6 cut(s) 107, 169, 221, 345, 502, 649
AlwI GGATC 2 cut(s) 583, 689
Aor13HI TCCGGA 1 cut(s) 292
AoxI GGCC 4 cut(s) 384, 906, 1136, 1217
ApeKI GCWGC 3 cut(s) 21, 38, 353
ApoI RAATTY 2 cut(s) 782, 1204
AspLEI GCGC 2 cut(s) 213, 484
AspS9I GGNCC 2 cut(s) 907, 1218
AsuHPI GGTGA 1 cut(s) 616
BaeGI GKGCMC 1 cut(s) 264
BanI GGYRCC 2 cut(s) 210, 259
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 4 cut(s) 264, 739, 785, 1068
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 3 cut(s) 8, 25, 340
BccI CCATC 5 cut(s) 56, 329, 398, 458, 1077
BceAI ACGGC 2 cut(s) 143, 371
BciT130I CCWGG 1 cut(s) 912
BcoDI GTCTC 6 cut(s) 107, 169, 221, 345, 502, 649
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 4 cut(s) 737, 789, 902, 1239
BfoI RGCGCY 1 cut(s) 214
BisI GCNGC 6 cut(s) 22, 39, 67, 354, 357, 520
BlsI GCNGC 6 cut(s) 23, 40, 68, 355, 358, 521
Bme1390I CCNGG 1 cut(s) 912
BmeRI GACNNNNNGTC 1 cut(s) 1072
BmgT120I GGNCC 2 cut(s) 907, 1218
BmiI GGNNCC 4 cut(s) 212, 261, 909, 1022
BmrFI CCNGG 1 cut(s) 912
BmsI GCATC 6 cut(s) 37, 439, 606, 818, 949, 1177
BpiI GAAGAC 4 cut(s) 264, 739, 785, 1068
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 2 cut(s) 431, 511
Bsa29I ATCGAT 3 cut(s) 653, 684, 1293
BsaHI GRCGYC 1 cut(s) 211
BsaI GGTCTC 3 cut(s) 221, 345, 649
BsaJI CCNNGG 3 cut(s) 30, 168, 910
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 1215
Bse1I ACTGG 2 cut(s) 952, 1031
Bse3DI GCAATG 2 cut(s) 798, 962
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 1 cut(s) 912
BseCI ATCGAT 3 cut(s) 653, 684, 1293
BseDI CCNNGG 3 cut(s) 30, 168, 910
BseGI GGATG 2 cut(s) 454, 466
BseLI CCNNNNNNNGG 2 cut(s) 223, 1215
BseMI GCAATG 2 cut(s) 798, 962
BseMII CTCAG 3 cut(s) 213, 732, 891
BseNI ACTGG 2 cut(s) 952, 1031
BseRI GAGGAG 3 cut(s) 239, 758, 759
BseSI GKGCMC 1 cut(s) 264
BseXI GCAGC 3 cut(s) 8, 25, 340
BseYI CCCAGC 1 cut(s) 593
BsgI GTGCAG 3 cut(s) 7, 410, 688
Bsh1236I CGCG 1 cut(s) 519
BshFI GGCC 4 cut(s) 386, 908, 1138, 1219
BshNI GGYRCC 2 cut(s) 210, 259
BshVI ATCGAT 3 cut(s) 653, 684, 1293
BsiSI CCGG 1 cut(s) 293
BslI CCNNNNNNNGG 2 cut(s) 223, 1215
BsmAI GTCTC 6 cut(s) 107, 169, 221, 345, 502, 649
BsnI GGCC 4 cut(s) 386, 908, 1138, 1219
Bso31I GGTCTC 3 cut(s) 221, 345, 649
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
Bsp19I CCATGG 2 cut(s) 30, 168
BspACI CCGC 8 cut(s) 66, 147, 156, 264, 356, 359, 505, 519
BspANI GGCC 4 cut(s) 386, 908, 1138, 1219
BspCNI CTCAG 3 cut(s) 214, 733, 890
BspDI ATCGAT 3 cut(s) 653, 684, 1293
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 1 cut(s) 519
BspLI GGNNCC 4 cut(s) 212, 261, 909, 1022
BspPI GGATC 2 cut(s) 583, 689
BspQI GCTCTTC 1 cut(s) 1249
BspT107I GGYRCC 2 cut(s) 210, 259
BspTNI GGTCTC 3 cut(s) 221, 345, 649
BsrBI CCGCTC 1 cut(s) 149
BsrDI GCAATG 2 cut(s) 798, 962
BsrI ACTGG 2 cut(s) 952, 1031
BssECI CCNNGG 3 cut(s) 30, 168, 910
BssMI GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
BssNAI GTATAC 1 cut(s) 1150
BssNI GRCGYC 1 cut(s) 211
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 2 cut(s) 30, 168
Bst1107I GTATAC 1 cut(s) 1150
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 1 cut(s) 1056
Bst6I CTCTTC 3 cut(s) 652, 1208, 1249
BstACI GRCGYC 1 cut(s) 211
BstC8I GCNNGC 2 cut(s) 264, 1179
BstDEI CTNAG 5 cut(s) 222, 344, 637, 741, 877
BstDSI CCRYGG 2 cut(s) 30, 168
BstENI CCTNNNNNAGG 1 cut(s) 1213
BstF5I GGATG 2 cut(s) 454, 466
BstFNI CGCG 1 cut(s) 519
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 2 cut(s) 213, 484
BstKTI GATC 6 cut(s) 591, 684, 688, 728, 864, 1293
BstMAI GTCTC 6 cut(s) 107, 169, 221, 345, 502, 649
BstMBI GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
BstMWI GCNNNNNNNGC 7 cut(s) 47, 155, 362, 713, 826, 1187, 1239
BstNI CCWGG 1 cut(s) 912
BstNSI RCATGY 1 cut(s) 1156
BstSCI CCNGG 1 cut(s) 910
BstSLI GKGCMC 1 cut(s) 264
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 3 cut(s) 8, 25, 340
BstV2I GAAGAC 4 cut(s) 264, 739, 785, 1068
BstXI CCANNNNNNTGG 1 cut(s) 253
BstZ17I GTATAC 1 cut(s) 1150
Bsu15I ATCGAT 3 cut(s) 653, 684, 1293
BsuRI GGCC 4 cut(s) 386, 908, 1138, 1219
BsuTUI ATCGAT 3 cut(s) 653, 684, 1293
BtgI CCRYGG 2 cut(s) 30, 168
BtsCI GGATG 2 cut(s) 454, 466
BtsIMutI CAGTG 4 cut(s) 219, 671, 929, 1280
Cac8I GCNNGC 2 cut(s) 264, 1179
CfoI GCGC 2 cut(s) 213, 484
Cfr13I GGNCC 2 cut(s) 907, 1218
ClaI ATCGAT 3 cut(s) 653, 684, 1293
Csp6I GTAC 1 cut(s) 585
CviAII CATG 4 cut(s) 31, 169, 302, 1153
CviQI GTAC 1 cut(s) 585
DdeI CTNAG 5 cut(s) 222, 344, 637, 741, 877
DinI GGCGCC 1 cut(s) 212
DpnI GATC 6 cut(s) 590, 683, 687, 727, 863, 1292
DpnII GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
DraIII CACNNNGTG 1 cut(s) 1132
DriI GACNNNNNGTC 1 cut(s) 1072
EaeI YGGCCR 1 cut(s) 384
Eam1104I CTCTTC 3 cut(s) 652, 1208, 1249
Eam1105I GACNNNNNGTC 1 cut(s) 1072
EarI CTCTTC 3 cut(s) 652, 1208, 1249
EciI GGCGGA 1 cut(s) 374
Eco130I CCWWGG 2 cut(s) 30, 168
Eco31I GGTCTC 3 cut(s) 221, 345, 649
Eco57I CTGAAG 3 cut(s) 55, 101, 1203
EcoNI CCTNNNNNAGG 1 cut(s) 1213
EcoO109I RGGNCCY 1 cut(s) 907
EcoRI GAATTC 1 cut(s) 782
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 30, 168
EcoT22I ATGCAT 1 cut(s) 964
EgeI GGCGCC 1 cut(s) 212
EheI GGCGCC 1 cut(s) 212
ErhI CCWWGG 2 cut(s) 30, 168
FaeI CATG 4 cut(s) 34, 172, 305, 1156
FalI AAGNNNNNCTT 6 cut(s) 10, 42, 178, 210, 809, 841
FatI CATG 4 cut(s) 30, 168, 301, 1152
FauI CCCGC 1 cut(s) 271
FblI GTMKAC 2 cut(s) 855, 1149
Fnu4HI GCNGC 6 cut(s) 22, 39, 67, 354, 357, 520
FokI GGATG 2 cut(s) 461, 473
Fsp4HI GCNGC 6 cut(s) 22, 39, 67, 354, 357, 520
FspBI CTAG 4 cut(s) 737, 789, 902, 1239
GlaI GCGC 2 cut(s) 212, 483
GluI GCNGC 6 cut(s) 22, 39, 67, 354, 357, 520
GsaI CCCAGC 1 cut(s) 597
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 4 cut(s) 386, 908, 1138, 1219
HapII CCGG 1 cut(s) 293
HhaI GCGC 2 cut(s) 213, 484
Hin1I GRCGYC 1 cut(s) 211
Hin1II CATG 4 cut(s) 34, 172, 305, 1156
Hin6I GCGC 2 cut(s) 211, 482
HinP1I GCGC 2 cut(s) 211, 482
HindIII AAGCTT 3 cut(s) 123, 192, 882
HinfI GANTC 4 cut(s) 510, 892, 1100, 1228
HpaII CCGG 1 cut(s) 293
HphI GGTGA 1 cut(s) 616
Hpy166II GTNNAC 4 cut(s) 607, 856, 1126, 1150
Hpy188I TCNGA 6 cut(s) 81, 757, 880, 978, 986, 1249
Hpy188III TCNNGA 6 cut(s) 293, 534, 729, 865, 902, 956
Hpy8I GTNNAC 4 cut(s) 607, 856, 1126, 1150
HpyAV CCTTC 8 cut(s) 88, 144, 425, 451, 495, 652, 926, 1090
HpyCH4III ACNGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 155, 362, 713, 826, 1187, 1239
HpyF3I CTNAG 5 cut(s) 222, 344, 637, 741, 877
Hsp92I GRCGYC 1 cut(s) 211
Hsp92II CATG 4 cut(s) 34, 172, 305, 1156
HspAI GCGC 2 cut(s) 211, 482
KasI GGCGCC 1 cut(s) 210
Kpn2I TCCGGA 1 cut(s) 292
Kzo9I GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
LguI GCTCTTC 1 cut(s) 1249
LmnI GCTCC 2 cut(s) 154, 935
Lsp1109I GCAGC 3 cut(s) 8, 25, 340
LweI GCATC 6 cut(s) 37, 439, 606, 818, 949, 1177
MaeI CTAG 4 cut(s) 737, 789, 902, 1239
MaeIII GTNAC 1 cut(s) 1158
MalI GATC 6 cut(s) 590, 683, 687, 727, 863, 1292
MbiI CCGCTC 1 cut(s) 149
MboI GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 4 cut(s) 782, 1191, 1204, 1257
Mly113I GGCGCC 1 cut(s) 211
MlyI GAGTC 1 cut(s) 504
MmeI TCCRAC 1 cut(s) 426
Mph1103I ATGCAT 1 cut(s) 964
MroI TCCGGA 1 cut(s) 292
MseI TTAA 3 cut(s) 315, 569, 918
MslI CAYNNNNRTG 3 cut(s) 29, 306, 602
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 1 cut(s) 912
MvaI CCWGG 1 cut(s) 912
MvnI CGCG 1 cut(s) 519
MwoI GCNNNNNNNGC 7 cut(s) 47, 155, 362, 713, 826, 1187, 1239
NarI GGCGCC 1 cut(s) 211
NcoI CCATGG 2 cut(s) 30, 168
NdeII GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
NlaIII CATG 4 cut(s) 34, 172, 305, 1156
NlaIV GGNNCC 4 cut(s) 212, 261, 909, 1022
NmeAIII GCCGAG 1 cut(s) 478
NsiI ATGCAT 1 cut(s) 964
NspI RCATGY 1 cut(s) 1156
PciSI GCTCTTC 1 cut(s) 1249
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PfeI GAWTC 3 cut(s) 892, 1100, 1228
PkrI GCNGC 6 cut(s) 23, 40, 68, 355, 358, 521
PleI GAGTC 1 cut(s) 504
PluTI GGCGCC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 504
Psp6I CCWGG 1 cut(s) 910
PspFI CCCAGC 1 cut(s) 593
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 4 cut(s) 212, 261, 909, 1022
PspPI GGNCC 2 cut(s) 907, 1218
RsaI GTAC 1 cut(s) 586
RsaNI GTAC 1 cut(s) 585
RseI CAYNNNNRTG 3 cut(s) 29, 306, 602
SapI GCTCTTC 1 cut(s) 1249
SaqAI TTAA 3 cut(s) 315, 569, 918
SatI GCNGC 6 cut(s) 22, 39, 67, 354, 357, 520
Sau3AI GATC 6 cut(s) 588, 681, 685, 725, 861, 1290
Sau96I GGNCC 2 cut(s) 907, 1218
SchI GAGTC 1 cut(s) 504
ScrFI CCNGG 1 cut(s) 912
SduI GDGCHC 1 cut(s) 264
SfaNI GCATC 6 cut(s) 37, 439, 606, 818, 949, 1177
SfoI GGCGCC 1 cut(s) 212
SmiMI CAYNNNNRTG 3 cut(s) 29, 306, 602
SmlI CTYRAG 2 cut(s) 410, 490
SmoI CTYRAG 2 cut(s) 410, 490
SpeI ACTAGT 1 cut(s) 788
Sse9I AATT 4 cut(s) 782, 1191, 1204, 1257
SsiI CCGC 8 cut(s) 66, 147, 156, 264, 356, 359, 505, 519
SspDI GGCGCC 1 cut(s) 210
SspMI CTAG 4 cut(s) 737, 789, 902, 1239
StyD4I CCNGG 1 cut(s) 910
StyI CCWWGG 2 cut(s) 30, 168
TaaI ACNGT 1 cut(s) 1056
TaqI TCGA 5 cut(s) 89, 653, 684, 864, 1293
TasI AATT 4 cut(s) 782, 1191, 1204, 1257
TauI GCSGC 3 cut(s) 69, 359, 522
TfiI GAWTC 3 cut(s) 892, 1100, 1228
Tru1I TTAA 3 cut(s) 315, 569, 918
Tru9I TTAA 3 cut(s) 315, 569, 918
TscAI CASTG 4 cut(s) 226, 671, 929, 1280
TseI GCWGC 3 cut(s) 21, 38, 353
TspDTI ATGAA 4 cut(s) 519, 639, 671, 1266
TspRI CASTG 4 cut(s) 226, 671, 929, 1280
XagI CCTNNNNNAGG 1 cut(s) 1213
XapI RAATTY 2 cut(s) 782, 1204
XbaI TCTAGA 1 cut(s) 901
XceI RCATGY 1 cut(s) 1156
XmiI GTMKAC 2 cut(s) 855, 1149
XspI CTAG 4 cut(s) 737, 789, 902, 1239
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.