pycom05g04170

DNA polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010240
Physical Location & Seq
Forward (+)
4899814 .. 4903301
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2409 bp
ATGATTGATGTACTTTGTACAAAGGATTCTTGGCTCTATAAAGTTGTGCAACATTCTCTTAAAACTTTAACTGATTGGGTTGGGGATGATGATGTGAGAAGGGTTTCTGTCATTGTGGCTCTACAGAAACATAGCAACGGAAAATTTGATTGCATTACTCGAACAAAAACAGTTAAGGATTTGATGTCAGATTTCAGAACAGAATCTGGTTGCATGCTGTTCATTCAGAACCTGTTGAACATGTTTCTGGATGAAAAGCATGCTACTGAGGAGCCTTCAGATCAGAGTCAAACGACAGATGACAATTCAGATATAGGTTCTGTTGAAGACAAGGAATCTGTTGGAACCATGGGAAATTCTGATTTTTTGAAAACATGGATTGTGGAATCCCTCCCCAGTATATTGAAAAATTTGAAACTGGATACTGAGGCAAAATTTAGGGTCCAAAAAGGAATTTTGAAGTTTCTAGCAGTTCAGGGTTTGTTCACCGCATCTCTTGGGTCTGAAGTAACATCCCTTGAATTACAAGAGACATTTAGGTGGCCAAAAACTGCCATCTCAAGTGCCCTTTGCAGGATGTGTATTGAGCAGCTCCAGTTACTGTTTGCAAACTCTCAGAAAGGAGAGGGGTCACGTCCCTTGCTAAATTGCGTAGAGCAAAGTGACCTTGGCTCATACTTTATGCGGTTCCTTAGCACTTTGTGCAGTATTCCTTCCGTCTCATATTTTCGGCCTTTGGAAACCGAGGAAGAAAACACTTTGAAAAAATTGCAAGCAATGGAAACTTCACTCTCAAAAGAGGAAAGGAATTGTCAGCTTACTAGTGATGCAAATAGGTTGCATGCACTAAGATACTTACTCATCCAGCTGCTTTTGCAAATGCTCCTTCGACCAAAGGAATACTTAGAAGCAGTCTCTGAGCTTATGATATGTTGTAAGAAAGCTTTTCCTGTTTCTGATCTTCTTGATGCCCCTGGAGAAGATGACTTGGATGATGAGGGTGCACCTGCCATGATGGATGTTCTTGTAGATACATTGCTTTCTTTGCTGCCAGAGTCATCAGCTCCCATGCGAACTGCAATCGAGCAGGTTTTTAAATGCTTCTGTGATGATATCACGGATGATGGTCTGTTGCGGATGATGAGGGTCATCCGGAAGAATTTGAAACCTGCTAGACATCAAGATGCTGATAGTGATGACATTTTTGATGATGAAGAAGATGATGAAGATTTTCTTAAAATTGAAGAAGATGAGGGAATTGACAAGGCTGAGACAGGTGAAACAGGTGACAGTGATGAGCAGCCAGATGACTCTGAGGCCGACTCTGAGGCTGCTGATGTGGTTGAGGCAGTTGGCAAAGAAAATCCTGAAGCTTCTGATGATTCTGATGGAGGAATGGATGACGATGCAATGTTTCGAATGGATACTTATCTTACCCAGATCTTCAAAGAGCGAAAGAATCTAGCTGGGGGTGACACTGCACATCACCAACTCATGCTGTTTAAGCTTCGTGTTCTTTCATTGCTAGAAATTTACCTACATGAAAATCCAGGCAAGCCGCAGGTTTTGTTGGTGTACTCAAACTTAGCTCGGGCATTTATTGAACCACCCAGTGCAGAAAGTAGTGAACAGCTAGGACAGCGTGTATGGGGGATACTGCAAAAGAAAATTTTTAAAGCAAAGGACTATCCGAAGGGTGAAGATGTGCAATTATCTACTTTAGAATCTTTGCTGCAAAAGAATCTGAAGTTGGCCTCAAAACCTATTAAGAGAAAGAAATCCGCAACCAACCTGTCCAAGAAGAAACAGTCAGCTTCGTGGAACCGACACAAAATGATTACTAGCCTTGCACAGAGCTCCACCTTCTGGATTCTGAAGATCGTTGAATCAAGAAATTTTCCAGAGTCTGAGCTGCAGAGGATTTTTGATATTTTCCAGGGAGTTCTAGTGGAGTATTTCAATAGTAAAAAGTCTCAAATCAAATCTGGATTTTTGAAAGAAATATTTAGAAGAAGGCCATGGGTAGGGCACCATCTGTTTGGCTTCCTGTTGGAGAAATGTGGCACTTCAAAGTCAGATTTTCGGCGAGTGGAAGCACTTGATTTGGTGAGTGAAATATTGAAGTCCCTGGGCTCCACTGATGGAAGTAGCCAGGAAGCGCTGAAGAATATCATGAAAAGCCATCTGCCGAAACTCTGTCGTCTGATAGAACACTTGCTGACAAATATATCAGAAAAACAGTCGAGGCAAGCTGAAGCGCGGAAGTTTTGTAGCAGGATCCTTCAGATGATAACAACCCTTAAGCTGACGAAGTCTTTCCTTAAAAACTTATCCCCAGATGCTCATGCCAAGTGTGAATCACAACTCAGTGCACAATTTATTAAAATGAAGACAGTACCACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000182 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003712 GO:0003714 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006139 GO:0006351 GO:0006355 GO:0006360 GO:0006725 GO:0006807 GO:0006915 GO:0006950 GO:0007154 GO:0007165 GO:0007623 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0008270 GO:0009058 GO:0009059 GO:0009267 GO:0009303 GO:0009605 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010564 GO:0010605 GO:0010629 GO:0010941 GO:0010942 GO:0012501 GO:0015980 GO:0016070 GO:0016072 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022900 GO:0022904 GO:0023052 GO:0031074 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031667 GO:0031668 GO:0031669 GO:0031974 GO:0031981 GO:0032501 GO:0032774 GO:0032922 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035556 GO:0042149 GO:0042564 GO:0042594 GO:0042790 GO:0042981 GO:0043065 GO:0043067 GO:0043068 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045333 GO:0045787 GO:0045892 GO:0045934 GO:0046483 GO:0046872 GO:0046914 GO:0048511 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0055114 GO:0060255 GO:0065007 GO:0070013 GO:0070888 GO:0071156 GO:0071158 GO:0071496 GO:0071704 GO:0072331 GO:0072332 GO:0080090 GO:0090068 GO:0090304 GO:0097159 GO:0097190 GO:0097193 GO:0097659 GO:0098781 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000209 GO:2000210 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DNA_pol_phi

Protein Analysis

803

Amino Acids

90.84

Weight (kDa)

5.33

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_phi PF04931 2 - 513 2.8e-98 DNA polymerase phi
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010240)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64420
fragaria_vesca FvH4_2g11160
malus_domestica MD05G1049800.v1.1 MD10G1056900.v1.1
prunus_persica Prupe.8G075100_v2.0.a1
pyrus_communis pycom05g04160 pycom05g04170 pycom10g04380
rosa_chinensis RchiOBHm_Chr6g0269481
rosa_laevigata RLG00000013848 RLG00000013849
rosa_multiflora Rmu_ssc0000291.1_g000033
rosa_roxburghii Rroxscaffold_7G00198700
rosa_rugosa Rorug06G0048500
rosa_samantha Rh6AG168400 Rh6BG173800 Rh6CG168000 Rh6CG168100 Rh6DG160000 Rh6DG160100
rosa_wichuraiana Rw6G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1015
Acc36I ACCTGC 4 cut(s) 1015, 1078, 1177, 1552
AccB1I GGYRCC 1 cut(s) 2028
AccB7I CCANNNNNTGG 1 cut(s) 1866
AccII CGCG 1 cut(s) 2260
AccIII TCCGGA 1 cut(s) 1152
AciI CCGC 6 cut(s) 489, 685, 1135, 1559, 1782, 2260
AclWI GGATC 2 cut(s) 2272, 2285
AcoI YGGCCR 1 cut(s) 542
AcsI RAATTY 9 cut(s) 143, 355, 409, 434, 453, 1159, 1530, 1668, 1894
AcuI CTGAAG 8 cut(s) 261, 525, 1389, 1766, 1895, 2183, 2267, 2274
AdeI CACNNNGTG 2 cut(s) 702, 1613
AfaI GTAC 4 cut(s) 12, 19, 1577, 2397
AfeI AGCGCT 1 cut(s) 2160
AfiI CCNNNNNNNGG 3 cut(s) 573, 1866, 2024
AflII CTTAAG 1 cut(s) 2300
AflIII ACRYGT 1 cut(s) 240
AhlI ACTAGT 1 cut(s) 821
AjiI CACGTC 1 cut(s) 635
AjnI CCWGG 5 cut(s) 973, 1549, 1935, 2127, 2151
AjuI GAANNNNNNNTTGG 2 cut(s) 1733, 1765
AleI CACNNNNGTG 1 cut(s) 2404
Alw21I GWGCWC 3 cut(s) 1006, 1859, 2374
Alw26I GTCTC 5 cut(s) 524, 724, 919, 1265, 1977
Alw44I GTGCAC 2 cut(s) 1002, 2370
AlwI GGATC 2 cut(s) 2272, 2285
AlwNI CAGNNNCTG 5 cut(s) 206, 232, 601, 917, 1907
Ama87I CYCGRG 1 cut(s) 1590
Aor13HI TCCGGA 1 cut(s) 1152
Aor51HI AGCGCT 1 cut(s) 2160
AoxI GGCC 5 cut(s) 542, 731, 1317, 1752, 2015
ApaLI GTGCAC 2 cut(s) 1002, 2370
ApeKI GCWGC 7 cut(s) 589, 868, 1048, 1300, 1331, 1732, 1912
ApoI RAATTY 9 cut(s) 143, 355, 409, 434, 453, 1159, 1530, 1668, 1894
Asp700I GAANNNNTTC 3 cut(s) 103, 1230, 2315
AspLEI GCGC 2 cut(s) 2161, 2260
AspS9I GGNCC 1 cut(s) 442
AsuHPI GGTGA 7 cut(s) 478, 1289, 1298, 1478, 1484, 1709, 2119
AsuII TTCGAA 1 cut(s) 1417
AvaI CYCGRG 1 cut(s) 1590
AvaII GGWCC 1 cut(s) 442
BaeGI GKGCMC 4 cut(s) 568, 1006, 2031, 2374
BalI TGGCCA 1 cut(s) 544
BamHI GGATCC 1 cut(s) 2277
BanI GGYRCC 1 cut(s) 2028
BanII GRGCYC 2 cut(s) 1859, 2135
BbsI GAAGAC 2 cut(s) 333, 2396
Bbv12I GWGCWC 3 cut(s) 1006, 1859, 2374
BbvI GCAGC 7 cut(s) 601, 855, 1035, 1312, 1318, 1719, 1899
BccI CCATC 7 cut(s) 563, 1009, 1118, 1382, 2040, 2135, 2190
BciT130I CCWGG 5 cut(s) 975, 1551, 1937, 2129, 2153
BciVI GTATCC 3 cut(s) 415, 1417, 1647
BcoDI GTCTC 5 cut(s) 524, 724, 919, 1265, 1977
BcuI ACTAGT 1 cut(s) 821
BfaI CTAG 8 cut(s) 467, 822, 1173, 1463, 1526, 1634, 1842, 1946
BfmI CTRYAG 2 cut(s) 122, 1913
BfoI RGCGCY 1 cut(s) 2162
BfrI CTTAAG 1 cut(s) 2300
BfuAI ACCTGC 4 cut(s) 1015, 1078, 1177, 1552
BfuI GTATCC 3 cut(s) 415, 1417, 1647
BglII AGATCT 1 cut(s) 1440
BisI GCNGC 8 cut(s) 590, 869, 1049, 1301, 1332, 1559, 1733, 1913
BlsI GCNGC 8 cut(s) 591, 870, 1050, 1302, 1333, 1560, 1734, 1914
Bme1390I CCNGG 5 cut(s) 975, 1551, 1937, 2129, 2153
Bme18I GGWCC 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 1590
BmgBI CACGTC 1 cut(s) 635
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 8 cut(s) 273, 346, 443, 689, 1823, 2030, 2134, 2279
BmrFI CCNGG 5 cut(s) 975, 1551, 1937, 2129, 2153
BmrI ACTGGG 2 cut(s) 390, 1605
BmsI GCATC 6 cut(s) 500, 817, 958, 1174, 1396, 2329
BmuI ACTGGG 2 cut(s) 390, 1605
BpiI GAAGAC 2 cut(s) 333, 2396
BplI GAGNNNNNCTC 2 cut(s) 1307, 1339
BpmI CTGGAG 2 cut(s) 578, 996
Bpu10I CCTNAGC 1 cut(s) 692
Bpu14I TTCGAA 1 cut(s) 1417
BpuEI CTTGAG 1 cut(s) 544
BsaBI GATNNNNATC 1 cut(s) 1428
BsaJI CCNNGG 8 cut(s) 348, 667, 744, 973, 1936, 2018, 2127, 2128
BsaWI WCCGGW 1 cut(s) 1152
BsaXI ACNNNNNCTCC 2 cut(s) 1932, 1962
Bsc4I CCNNNNNNNGG 3 cut(s) 573, 1866, 2024
Bse1I ACTGG 4 cut(s) 396, 423, 595, 1611
Bse3DI GCAATG 4 cut(s) 783, 1034, 1416, 1520
Bse8I GATNNNNATC 1 cut(s) 1428
BseAI TCCGGA 1 cut(s) 1152
BseBI CCWGG 5 cut(s) 975, 1551, 1937, 2129, 2153
BseDI CCNNGG 8 cut(s) 348, 667, 744, 973, 1936, 2018, 2127, 2128
BseJI GATNNNNATC 1 cut(s) 1428
BseLI CCNNNNNNNGG 3 cut(s) 573, 1866, 2024
BseMI GCAATG 4 cut(s) 783, 1034, 1416, 1520
BseMII CTCAG 9 cut(s) 258, 417, 629, 909, 1260, 1305, 1317, 1899, 2380
BseNI ACTGG 4 cut(s) 396, 423, 595, 1611
BseRI GAGGAG 1 cut(s) 284
BseSI GKGCMC 4 cut(s) 568, 1006, 2031, 2374
BseXI GCAGC 7 cut(s) 601, 855, 1035, 1312, 1318, 1719, 1899
BseYI CCCAGC 1 cut(s) 1466
BsgI GTGCAG 3 cut(s) 724, 1464, 1635
Bsh1236I CGCG 1 cut(s) 2260
BshFI GGCC 5 cut(s) 544, 733, 1319, 1754, 2017
BshNI GGYRCC 1 cut(s) 2028
BsiHKAI GWGCWC 3 cut(s) 1006, 1859, 2374
BsiHKCI CYCGRG 1 cut(s) 1590
BsiSI CCGG 1 cut(s) 1153
BslFI GGGAC 2 cut(s) 621, 2110
BslI CCNNNNNNNGG 3 cut(s) 573, 1866, 2024
BsmAI GTCTC 5 cut(s) 524, 724, 919, 1265, 1977
BsmBI CGTCTC 1 cut(s) 724
BsmFI GGGAC 2 cut(s) 621, 2110
BsnI GGCC 5 cut(s) 544, 733, 1319, 1754, 2017
BsoBI CYCGRG 1 cut(s) 1590
Bsp119I TTCGAA 1 cut(s) 1417
Bsp1286I GDGCHC 6 cut(s) 568, 1006, 1859, 2031, 2135, 2374
Bsp13I TCCGGA 1 cut(s) 1152
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 5 cut(s) 280, 958, 1440, 1878, 2277
Bsp19I CCATGG 2 cut(s) 348, 2018
BspACI CCGC 6 cut(s) 489, 685, 1135, 1559, 1782, 2260
BspANI GGCC 5 cut(s) 544, 733, 1319, 1754, 2017
BspCNI CTCAG 9 cut(s) 259, 418, 628, 910, 1261, 1306, 1318, 1900, 2379
BspEI TCCGGA 1 cut(s) 1152
BspFNI CGCG 1 cut(s) 2260
BspHI TCATGA 1 cut(s) 2172
BspLI GGNNCC 8 cut(s) 273, 346, 443, 689, 1823, 2030, 2134, 2279
BspMAI CTGCAG 1 cut(s) 1917
BspMI ACCTGC 4 cut(s) 1015, 1078, 1177, 1552
BspPI GGATC 2 cut(s) 2272, 2285
BspT104I TTCGAA 1 cut(s) 1417
BspT107I GGYRCC 1 cut(s) 2028
BspTI CTTAAG 1 cut(s) 2300
BsrDI GCAATG 4 cut(s) 783, 1034, 1416, 1520
BsrGI TGTACA 1 cut(s) 17
BsrI ACTGG 4 cut(s) 396, 423, 595, 1611
BssECI CCNNGG 8 cut(s) 348, 667, 744, 973, 1936, 2018, 2127, 2128
BssMI GATC 5 cut(s) 280, 958, 1440, 1878, 2277
BssT1I CCWWGG 3 cut(s) 348, 667, 2018
Bst2UI CCWGG 5 cut(s) 975, 1551, 1937, 2129, 2153
Bst4CI ACNGT 6 cut(s) 172, 603, 1292, 1809, 2241, 2395
BstAFI CTTAAG 1 cut(s) 2300
BstAPI GCANNNNNTGC 1 cut(s) 702
BstAUI TGTACA 1 cut(s) 17
BstBI TTCGAA 1 cut(s) 1417
BstC8I GCNNGC 6 cut(s) 215, 261, 774, 843, 1556, 2250
BstDSI CCRYGG 2 cut(s) 348, 2018
BstFNI CGCG 1 cut(s) 2260
BstH2I RGCGCY 1 cut(s) 2162
BstHHI GCGC 2 cut(s) 2161, 2260
BstKTI GATC 5 cut(s) 283, 961, 1443, 1881, 2280
BstMAI GTCTC 5 cut(s) 524, 724, 919, 1265, 1977
BstMBI GATC 5 cut(s) 280, 958, 1440, 1878, 2277
BstMWI GCNNNNNNNGC 5 cut(s) 702, 874, 1045, 1504, 1639
BstNI CCWGG 5 cut(s) 975, 1551, 1937, 2129, 2153
BstNSI RCATGY 4 cut(s) 217, 244, 263, 845
BstSCI CCNGG 5 cut(s) 973, 1549, 1935, 2127, 2151
BstSFI CTRYAG 2 cut(s) 122, 1913
BstSLI GKGCMC 4 cut(s) 568, 1006, 2031, 2374
BstUI CGCG 1 cut(s) 2260
BstV1I GCAGC 7 cut(s) 601, 855, 1035, 1312, 1318, 1719, 1899
BstV2I GAAGAC 2 cut(s) 333, 2396
BstX2I RGATCY 2 cut(s) 1440, 2277
BstXI CCANNNNNNTGG 1 cut(s) 2039
BstYI RGATCY 2 cut(s) 1440, 2277
BsuI GTATCC 3 cut(s) 415, 1417, 1647
BsuRI GGCC 5 cut(s) 544, 733, 1319, 1754, 2017
BtgI CCRYGG 2 cut(s) 348, 2018
BtrI CACGTC 1 cut(s) 635
BtsI GCAGTG 1 cut(s) 1476
BtsIMutI CAGTG 5 cut(s) 1297, 1476, 1618, 2136, 2374
BveI ACCTGC 4 cut(s) 1015, 1078, 1177, 1552
Cac8I GCNNGC 6 cut(s) 215, 261, 774, 843, 1556, 2250
CaiI CAGNNNCTG 5 cut(s) 206, 232, 601, 917, 1907
CciI TCATGA 1 cut(s) 2172
CfoI GCGC 2 cut(s) 2161, 2260
Cfr13I GGNCC 1 cut(s) 442
Csp6I GTAC 4 cut(s) 11, 18, 1576, 2396
CviQI GTAC 4 cut(s) 11, 18, 1576, 2396
DpnI GATC 5 cut(s) 282, 960, 1442, 1880, 2279
DpnII GATC 5 cut(s) 280, 958, 1440, 1878, 2277
DraI TTTAAA 2 cut(s) 1096, 1675
DraIII CACNNNGTG 2 cut(s) 702, 1613
EaeI YGGCCR 1 cut(s) 542
Ecl136II GAGCTC 1 cut(s) 1857
Eco130I CCWWGG 3 cut(s) 348, 667, 2018
Eco24I GRGCYC 2 cut(s) 1859, 2135
Eco32I GATATC 1 cut(s) 1114
Eco47I GGWCC 1 cut(s) 442
Eco47III AGCGCT 1 cut(s) 2160
Eco53kI GAGCTC 1 cut(s) 1857
Eco57I CTGAAG 8 cut(s) 261, 525, 1389, 1766, 1895, 2183, 2267, 2274
Eco88I CYCGRG 1 cut(s) 1590
EcoICRI GAGCTC 1 cut(s) 1857
EcoRII CCWGG 5 cut(s) 973, 1549, 1935, 2127, 2151
EcoRV GATATC 1 cut(s) 1114
EcoT14I CCWWGG 3 cut(s) 348, 667, 2018
EcoT38I GRGCYC 2 cut(s) 1859, 2135
ErhI CCWWGG 3 cut(s) 348, 667, 2018
Esp3I CGTCTC 1 cut(s) 724
FalI AAGNNNNNCTT 4 cut(s) 887, 919, 1218, 1250
FaqI GGGAC 2 cut(s) 621, 2110
Fnu4HI GCNGC 8 cut(s) 590, 869, 1049, 1301, 1332, 1559, 1733, 1913
FriOI GRGCYC 2 cut(s) 1859, 2135
Fsp4HI GCNGC 8 cut(s) 590, 869, 1049, 1301, 1332, 1559, 1733, 1913
FspBI CTAG 8 cut(s) 467, 822, 1173, 1463, 1526, 1634, 1842, 1946
GlaI GCGC 2 cut(s) 2160, 2259
GluI GCNGC 8 cut(s) 590, 869, 1049, 1301, 1332, 1559, 1733, 1913
GsaI CCCAGC 1 cut(s) 1470
GsuI CTGGAG 2 cut(s) 578, 996
HaeII RGCGCY 1 cut(s) 2162
HaeIII GGCC 5 cut(s) 544, 733, 1319, 1754, 2017
HapII CCGG 1 cut(s) 1153
HhaI GCGC 2 cut(s) 2161, 2260
Hin6I GCGC 2 cut(s) 2159, 2258
HinP1I GCGC 2 cut(s) 2159, 2258
HindIII AAGCTT 3 cut(s) 942, 1371, 1505
HpaII CCGG 1 cut(s) 1153
HphI GGTGA 7 cut(s) 478, 1289, 1298, 1478, 1484, 1709, 2119
Hpy166II GTNNAC 5 cut(s) 486, 1004, 1576, 1628, 2372
Hpy8I GTNNAC 5 cut(s) 486, 1004, 1576, 1628, 2372
HpyAV CCTTC 8 cut(s) 93, 285, 723, 896, 1687, 1873, 2007, 2291
HpyCH4III ACNGT 6 cut(s) 172, 603, 1292, 1809, 2241, 2395
HpyCH4IV ACGT 1 cut(s) 634
HpyF10VI GCNNNNNNNGC 5 cut(s) 702, 874, 1045, 1504, 1639
HpySE526I ACGT 1 cut(s) 634
HspAI GCGC 2 cut(s) 2159, 2258
Kpn2I TCCGGA 1 cut(s) 1152
Kzo9I GATC 5 cut(s) 280, 958, 1440, 1878, 2277
LmnI GCTCC 6 cut(s) 271, 597, 888, 1069, 1862, 2138
Lsp1109I GCAGC 7 cut(s) 601, 855, 1035, 1312, 1318, 1719, 1899
LweI GCATC 6 cut(s) 500, 817, 958, 1174, 1396, 2329
MaeI CTAG 8 cut(s) 467, 822, 1173, 1463, 1526, 1634, 1842, 1946
MaeII ACGT 1 cut(s) 634
MaeIII GTNAC 6 cut(s) 508, 597, 630, 662, 1286, 1472
MalI GATC 5 cut(s) 282, 960, 1442, 1880, 2279
MboI GATC 5 cut(s) 280, 958, 1440, 1878, 2277
MflI RGATCY 2 cut(s) 1440, 2277
MhlI GDGCHC 6 cut(s) 568, 1006, 1859, 2031, 2135, 2374
MlsI TGGCCA 1 cut(s) 544
MluNI TGGCCA 1 cut(s) 544
MlyI GAGTC 5 cut(s) 295, 1064, 1304, 1316, 1913
MmeI TCCRAC 2 cut(s) 322, 2031
Mox20I TGGCCA 1 cut(s) 544
MroI TCCGGA 1 cut(s) 1152
MroXI GAANNNNTTC 3 cut(s) 103, 1230, 2315
MscI TGGCCA 1 cut(s) 544
MslI CAYNNNNRTG 3 cut(s) 538, 1182, 2404
Msp20I TGGCCA 1 cut(s) 544
MspA1I CMGCKG 1 cut(s) 868
MspCI CTTAAG 1 cut(s) 2300
MspI CCGG 1 cut(s) 1153
MspR9I CCNGG 5 cut(s) 975, 1551, 1937, 2129, 2153
MvaI CCWGG 5 cut(s) 975, 1551, 1937, 2129, 2153
MvnI CGCG 1 cut(s) 2260
MwoI GCNNNNNNNGC 5 cut(s) 702, 874, 1045, 1504, 1639
NcoI CCATGG 2 cut(s) 348, 2018
NdeII GATC 5 cut(s) 280, 958, 1440, 1878, 2277
NlaIV GGNNCC 8 cut(s) 273, 346, 443, 689, 1823, 2030, 2134, 2279
NmuCI GTSAC 4 cut(s) 630, 662, 1286, 1472
NspI RCATGY 4 cut(s) 217, 244, 263, 845
NspV TTCGAA 1 cut(s) 1417
OliI CACNNNNGTG 1 cut(s) 2404
PaeI GCATGC 3 cut(s) 217, 263, 845
PagI TCATGA 1 cut(s) 2172
PaqCI CACCTGC 1 cut(s) 1015
PasI CCCWGGG 1 cut(s) 2128
PciI ACATGT 1 cut(s) 240
PdmI GAANNNNTTC 3 cut(s) 103, 1230, 2315
PflFI GACNNNGTC 1 cut(s) 2311
PflMI CCANNNNNTGG 1 cut(s) 1866
PkrI GCNGC 8 cut(s) 591, 870, 1050, 1302, 1333, 1560, 1734, 1914
PleI GAGTC 5 cut(s) 294, 1063, 1304, 1316, 1912
PpsI GAGTC 5 cut(s) 294, 1063, 1304, 1316, 1912
PscI ACATGT 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 1859
Psp6I CCWGG 5 cut(s) 973, 1549, 1935, 2127, 2151
PspFI CCCAGC 1 cut(s) 1466
PspGI CCWGG 5 cut(s) 973, 1549, 1935, 2127, 2151
PspN4I GGNNCC 8 cut(s) 273, 346, 443, 689, 1823, 2030, 2134, 2279
PspPI GGNCC 1 cut(s) 442
PstI CTGCAG 1 cut(s) 1917
PstNI CAGNNNCTG 5 cut(s) 206, 232, 601, 917, 1907
PsuI RGATCY 2 cut(s) 1440, 2277
PsyI GACNNNGTC 1 cut(s) 2311
PvuII CAGCTG 1 cut(s) 868
RsaI GTAC 4 cut(s) 12, 19, 1577, 2397
RsaNI GTAC 4 cut(s) 11, 18, 1576, 2396
RseI CAYNNNNRTG 3 cut(s) 538, 1182, 2404
SacI GAGCTC 1 cut(s) 1859
SatI GCNGC 8 cut(s) 590, 869, 1049, 1301, 1332, 1559, 1733, 1913
Sau3AI GATC 5 cut(s) 280, 958, 1440, 1878, 2277
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 5 cut(s) 295, 1064, 1304, 1316, 1913
ScrFI CCNGG 5 cut(s) 975, 1551, 1937, 2129, 2153
SduI GDGCHC 6 cut(s) 568, 1006, 1859, 2031, 2135, 2374
SfaNI GCATC 6 cut(s) 500, 817, 958, 1174, 1396, 2329
SfcI CTRYAG 2 cut(s) 122, 1913
SfuI TTCGAA 1 cut(s) 1417
SinI GGWCC 1 cut(s) 442
SmiMI CAYNNNNRTG 3 cut(s) 538, 1182, 2404
SmlI CTYRAG 2 cut(s) 559, 2300
SmoI CTYRAG 2 cut(s) 559, 2300
SpeI ACTAGT 1 cut(s) 821
SphI GCATGC 3 cut(s) 217, 263, 845
SsiI CCGC 6 cut(s) 489, 685, 1135, 1559, 1782, 2260
SspI AATATT 2 cut(s) 2004, 2118
SspMI CTAG 8 cut(s) 467, 822, 1173, 1463, 1526, 1634, 1842, 1946
SstI GAGCTC 1 cut(s) 1859
StyD4I CCNGG 5 cut(s) 973, 1549, 1935, 2127, 2151
StyI CCWWGG 3 cut(s) 348, 667, 2018
TaaI ACNGT 6 cut(s) 172, 603, 1292, 1809, 2241, 2395
TaiI ACGT 1 cut(s) 637
TaqI TCGA 5 cut(s) 160, 889, 1083, 1417, 2243
TatI WGTACW 3 cut(s) 10, 17, 1575
TauI GCSGC 1 cut(s) 1561
TscAI CASTG 5 cut(s) 1297, 1483, 1618, 2143, 2374
TseFI GTSAC 4 cut(s) 630, 662, 1286, 1472
TseI GCWGC 7 cut(s) 589, 868, 1048, 1300, 1331, 1732, 1912
Tsp45I GTSAC 4 cut(s) 630, 662, 1286, 1472
TspDTI ATGAA 8 cut(s) 211, 267, 1227, 1239, 1509, 1557, 2189, 2402
TspGWI ACGGA 3 cut(s) 153, 706, 1133
TspRI CASTG 5 cut(s) 1297, 1483, 1618, 2143, 2374
Tth111I GACNNNGTC 1 cut(s) 2311
Van91I CCANNNNNTGG 1 cut(s) 1866
Vha464I CTTAAG 1 cut(s) 2300
VneI GTGCAC 2 cut(s) 1002, 2370
VpaK11BI GGWCC 1 cut(s) 442
XapI RAATTY 9 cut(s) 143, 355, 409, 434, 453, 1159, 1530, 1668, 1894
XceI RCATGY 4 cut(s) 217, 244, 263, 845
XmnI GAANNNNTTC 3 cut(s) 103, 1230, 2315
XspI CTAG 8 cut(s) 467, 822, 1173, 1463, 1526, 1634, 1842, 1946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.