pycom05g06730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010427
Physical Location & Seq
Forward (+)
9413688 .. 9415812
2125 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g06730.1

Sequence Viewer

Length: 783 bp
ATGGCGACCCTTTATCCGTCTCACTTGTTATCTGCGTCCTCCCACCTCCAATCCTCCACTAAAATGGCGTTCGGGCCTCACCCTTCTCCTTCTTCCCATGGGCTCAAGATGGGTTTGGCACAGTGTAATGAAATACATGCCTTTGGTCAGAGTTTTAAGCCTTATGGGCTGCGTTCAAATTCTCGGCACAATATCTTGCATGCGATAAATATGACTGCAGGACAGTCAGATGACTCAGGGAAGATGAAATTTGATCAGCTGATTGATAAAGCAAGACAAGTGTGGGAAAGTTCTCCACAACCTGTCAAGAGCTTCCCTTGGAAAAGAGCAGCACAAAACTTTGTTCAACTCATCATAGACCTTATACTAGCAGTTGTCAAGTACTTGTGTGTACCTCTATTTGCAGTTTCCTCGCTTAGTGAAATGTCTTACTGTGCTCATGAAAGGAAGTTGTTTTTCATTCCTATTCCTGTCCTCATTGGAATGGCTATTGCTGAAGTCTTTAAACTGGCAGCACTGGATGCTTCTCCACGTCTTAAGGATGCAGAAGTTCCCTGGCATCTGATTCTTATGGCAATTTTCTTCACACTGCTCAAATTGCCGGGTCCATATTACCCATTTTGGGGGAGAATATTAATCCCACACTTTGCTAATGGGGGTTTGTTGAGGACATTATGGCTTGCGTTTTTGTGGTACAGAAGACCTCAAAAGGCATTGAGAATGGCACCATCACAACATGCTGAAAGTGACAGTCGGTCTGAAGTTGAGCCAAATAAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

261

Amino Acids

29.27

Weight (kDa)

9.63

Isoelectric Point (pI)

65.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49510 AT1G49510 AT1G49510
fragaria_vesca FvH4_2g06990 FvH4_2g07010
malus_domestica MD00G1050600.v1.1
prunus_persica Prupe.8G115700_v2.0.a1
pyrus_communis pycom05g06730
rosa_chinensis RchiOBHm_Chr6g0259351
rosa_laevigata RLG00000014537
rosa_multiflora Rmu_ssc0000050.1_g000059
rosa_roxburghii Rroxscaffold_7G00206590
rosa_rugosa Rorug05G0587800 Rorug05G0587900.1 Rorug05G0588000 Rorug05G0588100
rosa_samantha Rh6AG103800 Rh6BG097000 Rh6CG093200 Rh6DG087000
rosa_wichuraiana Rw6G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 724
AcsI RAATTY 2 cut(s) 178, 248
AcuI CTGAAG 2 cut(s) 516, 780
AfaI GTAC 3 cut(s) 383, 393, 695
AfiI CCNNNNNNNGG 2 cut(s) 622, 623
AflII CTTAAG 1 cut(s) 536
AgsI TTSAA 2 cut(s) 177, 347
AhdI GACNNNNNGTC 1 cut(s) 754
AjiI CACGTC 1 cut(s) 533
AjnI CCWGG 1 cut(s) 554
AluBI AGCT 3 cut(s) 259, 312, 778
AluI AGCT 3 cut(s) 259, 312, 778
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 1 cut(s) 24
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 3 cut(s) 169, 329, 512
ApoI RAATTY 2 cut(s) 178, 248
AseI ATTAAT 1 cut(s) 635
AspS9I GGNCC 2 cut(s) 74, 605
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 1 cut(s) 71
AvaII GGWCC 1 cut(s) 605
BanI GGYRCC 1 cut(s) 724
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 706
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 3 cut(s) 156, 341, 524
BccI CCATC 2 cut(s) 103, 736
BcgI CGANNNNNNTGC 2 cut(s) 393, 427
BciT130I CCWGG 1 cut(s) 556
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 2 cut(s) 368, 781
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 536
BglI GCCNNNNNGGC 1 cut(s) 166
BisI GCNGC 3 cut(s) 170, 330, 513
BlsI GCNGC 3 cut(s) 171, 331, 514
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 2 cut(s) 556, 603
Bme18I GGWCC 1 cut(s) 605
BmeRI GACNNNNNGTC 1 cut(s) 754
BmgBI CACGTC 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 74, 605
BmiI GGNNCC 2 cut(s) 606, 726
BmrFI CCNGG 2 cut(s) 556, 603
BmsI GCATC 3 cut(s) 511, 532, 568
BpiI GAAGAC 1 cut(s) 706
BpuEI CTTGAG 1 cut(s) 89
BpuMI CCSGG 1 cut(s) 603
BsaJI CCNNGG 3 cut(s) 97, 317, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 622, 623
Bse1I ACTGG 2 cut(s) 513, 522
BseBI CCWGG 1 cut(s) 556
BseDI CCNNGG 3 cut(s) 97, 317, 554
BseGI GGATG 2 cut(s) 526, 547
BseLI CCNNNNNNNGG 2 cut(s) 622, 623
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 2 cut(s) 513, 522
BseXI GCAGC 3 cut(s) 156, 341, 524
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 724
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 622, 623
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 2 cut(s) 105, 439
Bsp143I GATC 1 cut(s) 253
Bsp19I CCATGG 1 cut(s) 97
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 248
BspHI TCATGA 1 cut(s) 439
BspLI GGNNCC 2 cut(s) 606, 726
BspMAI CTGCAG 1 cut(s) 220
BspT107I GGYRCC 1 cut(s) 724
BspTI CTTAAG 1 cut(s) 536
BsrI ACTGG 2 cut(s) 513, 522
BssECI CCNNGG 3 cut(s) 97, 317, 554
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 2 cut(s) 97, 317
Bst2UI CCWGG 1 cut(s) 556
Bst4CI ACNGT 4 cut(s) 123, 225, 434, 752
BstAFI CTTAAG 1 cut(s) 536
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 2 cut(s) 201, 681
BstDEI CTNAG 2 cut(s) 235, 416
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 2 cut(s) 526, 547
BstKTI GATC 1 cut(s) 256
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 4 cut(s) 166, 521, 598, 775
BstNI CCWGG 1 cut(s) 556
BstNSI RCATGY 3 cut(s) 140, 203, 740
BstSCI CCNGG 2 cut(s) 554, 601
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 3 cut(s) 156, 341, 524
BstV2I GAAGAC 1 cut(s) 706
BstXI CCANNNNNNTGG 1 cut(s) 64
BsuRI GGCC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 97
BtrI CACGTC 1 cut(s) 533
BtsCI GGATG 2 cut(s) 526, 547
BtsI GCAGTG 1 cut(s) 587
BtsIMutI CAGTG 3 cut(s) 128, 515, 587
Cac8I GCNNGC 2 cut(s) 201, 681
CciI TCATGA 1 cut(s) 439
Cfr13I GGNCC 2 cut(s) 74, 605
CseI GACGC 1 cut(s) 24
Csp6I GTAC 3 cut(s) 382, 392, 694
CviAII CATG 5 cut(s) 98, 137, 200, 440, 737
CviQI GTAC 3 cut(s) 382, 392, 694
DdeI CTNAG 2 cut(s) 235, 416
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
DraI TTTAAA 1 cut(s) 505
DriI GACNNNNNGTC 1 cut(s) 754
Eam1105I GACNNNNNGTC 1 cut(s) 754
Eco130I CCWWGG 2 cut(s) 97, 317
Eco24I GRGCYC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 2 cut(s) 516, 780
EcoRII CCWGG 1 cut(s) 554
EcoT14I CCWWGG 2 cut(s) 97, 317
EcoT38I GRGCYC 1 cut(s) 105
ErhI CCWWGG 2 cut(s) 97, 317
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 5 cut(s) 101, 140, 203, 443, 740
FatI CATG 5 cut(s) 97, 136, 199, 439, 736
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 3 cut(s) 170, 330, 513
FokI GGATG 2 cut(s) 533, 554
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 3 cut(s) 170, 330, 513
FspBI CTAG 2 cut(s) 368, 781
GluI GCNGC 3 cut(s) 170, 330, 513
HaeIII GGCC 1 cut(s) 76
HapII CCGG 1 cut(s) 602
HgaI GACGC 1 cut(s) 24
Hin1II CATG 5 cut(s) 101, 140, 203, 443, 740
HinfI GANTC 2 cut(s) 233, 565
HpaII CCGG 1 cut(s) 602
HphI GGTGA 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 4 cut(s) 150, 229, 564, 760
Hpy188III TCNNGA 3 cut(s) 106, 307, 440
Hpy8I GTNNAC 1 cut(s) 392
HpyAV CCTTC 2 cut(s) 93, 99
HpyCH4III ACNGT 4 cut(s) 123, 225, 434, 752
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 4 cut(s) 199, 218, 404, 545
HpyF10VI GCNNNNNNNGC 4 cut(s) 166, 521, 598, 775
HpyF3I CTNAG 2 cut(s) 235, 416
HpySE526I ACGT 1 cut(s) 532
Hsp92II CATG 5 cut(s) 101, 140, 203, 443, 740
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 1 cut(s) 253
LpnPI CCDG 9 cut(s) 204, 222, 315, 483, 494, 503, 541, 568, 615
Lsp1109I GCAGC 3 cut(s) 156, 341, 524
LweI GCATC 3 cut(s) 511, 532, 568
MaeI CTAG 2 cut(s) 368, 781
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 1 cut(s) 746
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 4 cut(s) 84, 253, 574, 711
MhlI GDGCHC 2 cut(s) 105, 439
MluCI AATT 4 cut(s) 178, 248, 576, 596
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 9 cut(s) 49, 56, 64, 87, 405, 421, 485, 660, 714
MseI TTAA 4 cut(s) 156, 504, 537, 635
MslI CAYNNNNRTG 2 cut(s) 62, 482
MspA1I CMGCKG 1 cut(s) 259
MspCI CTTAAG 1 cut(s) 536
MspI CCGG 1 cut(s) 602
MspR9I CCNGG 2 cut(s) 556, 603
MvaI CCWGG 1 cut(s) 556
MwoI GCNNNNNNNGC 4 cut(s) 166, 521, 598, 775
NciI CCSGG 1 cut(s) 603
NcoI CCATGG 1 cut(s) 97
NdeII GATC 1 cut(s) 253
NlaIII CATG 5 cut(s) 101, 140, 203, 443, 740
NlaIV GGNNCC 2 cut(s) 606, 726
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 746
NspI RCATGY 3 cut(s) 140, 203, 740
PaeI GCATGC 1 cut(s) 203
PagI TCATGA 1 cut(s) 439
PfeI GAWTC 1 cut(s) 565
PkrI GCNGC 3 cut(s) 171, 331, 514
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PshBI ATTAAT 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 554
PspGI CCWGG 1 cut(s) 554
PspN4I GGNNCC 2 cut(s) 606, 726
PspPI GGNCC 2 cut(s) 74, 605
PstI CTGCAG 1 cut(s) 220
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 383, 393, 695
RsaNI GTAC 3 cut(s) 382, 392, 694
RseI CAYNNNNRTG 2 cut(s) 62, 482
SaqAI TTAA 4 cut(s) 156, 504, 537, 635
SatI GCNGC 3 cut(s) 170, 330, 513
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 2 cut(s) 74, 605
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 556, 603
SduI GDGCHC 2 cut(s) 105, 439
SetI ASST 9 cut(s) 48, 261, 304, 314, 363, 397, 535, 706, 780
SfaNI GCATC 3 cut(s) 511, 532, 568
SfcI CTRYAG 1 cut(s) 216
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 2 cut(s) 62, 482
SmlI CTYRAG 2 cut(s) 104, 536
SmoI CTYRAG 2 cut(s) 104, 536
SphI GCATGC 1 cut(s) 203
Sse9I AATT 4 cut(s) 178, 248, 576, 596
SspI AATATT 1 cut(s) 633
SspMI CTAG 2 cut(s) 368, 781
StyD4I CCNGG 2 cut(s) 554, 601
StyI CCWWGG 2 cut(s) 97, 317
TaaI ACNGT 4 cut(s) 123, 225, 434, 752
TaiI ACGT 1 cut(s) 535
TaqII GACCGA 1 cut(s) 744
TasI AATT 4 cut(s) 178, 248, 576, 596
TatI WGTACW 1 cut(s) 381
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 4 cut(s) 156, 504, 537, 635
Tru9I TTAA 4 cut(s) 156, 504, 537, 635
TscAI CASTG 3 cut(s) 128, 522, 594
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 3 cut(s) 169, 329, 512
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 4 cut(s) 144, 260, 448, 456
TspGWI ACGGA 1 cut(s) 6
TspRI CASTG 3 cut(s) 128, 522, 594
Vha464I CTTAAG 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 605
VspI ATTAAT 1 cut(s) 635
XapI RAATTY 2 cut(s) 178, 248
XceI RCATGY 3 cut(s) 140, 203, 740
XspI CTAG 2 cut(s) 368, 781
ZrmI AGTACT 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.