Rmu_ssc0000050.1_g000059

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000050.1
OG0010427
Physical Location & Seq
Forward (+)
276921 .. 279109
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000050.1_g000059.1.cds

Sequence Viewer

Length: 777 bp
atgacgactctctatccttgtcacttgtcctctgcttccgcccacctccaggcctccgccaaattcgccgcccttaacaataagcctcccaattccactcatggcttcaagggtttggcacagtttagtgaagtacatgcctttggtctgagtgttaagccttctcgtttgcttcagaattcccggtacaatatctcatgtgctgtgaacatgactgctggacagtcggatgaccctgggcagataaaatttgatcacctaatcgctaaagcaagaaagttatgggaaagttctccacagccagtcaagatgttcccttggaaaagagcagggctcaactttattcaactcatccttgaccttgtactagcagttgctaaatacttatgtgtacctctgctagtagtttcatctcttagtgagatgtcctactgtgcacaggaaaggaagttgtttttcgttcctgtacctgtcatcattggaatggctattgctgaagttttaaaactggcagctttggatgtttctccacttctgaaggatgcagaaattccctggcatctgtttgtcatggcaattttcttcacactgctcaaattgccaggtccatattacccattctggggccgaataataatcccacacattgccaatgggggtttggtaaggacattgtggtttgcatttctgtggtacagaagacctcggaaggcatcaacaacagcatcatcacaccattctgacagtcgcagtcaatctgaaccaaatgaactctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

28.79

Weight (kDa)

9.64

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49510 AT1G49510 AT1G49510
fragaria_vesca FvH4_2g06990 FvH4_2g07010
malus_domestica MD00G1050600.v1.1
prunus_persica Prupe.8G115700_v2.0.a1
pyrus_communis pycom05g06730
rosa_chinensis RchiOBHm_Chr6g0259351
rosa_laevigata RLG00000014537
rosa_multiflora Rmu_ssc0000050.1_g000059
rosa_roxburghii Rroxscaffold_7G00206590
rosa_rugosa Rorug05G0587800 Rorug05G0587900.1 Rorug05G0588000 Rorug05G0588100
rosa_samantha Rh6AG103800 Rh6BG097000 Rh6CG093200 Rh6DG087000
rosa_wichuraiana Rw6G008960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 39, 57, 69
AcsI RAATTY 4 cut(s) 62, 178, 248, 549
AcuI CTGAAG 3 cut(s) 158, 516, 557
AfaI GTAC 6 cut(s) 135, 188, 366, 393, 468, 695
AfiI CCNNNNNNNGG 3 cut(s) 49, 622, 623
AgsI TTSAA 2 cut(s) 109, 347
AjnI CCWGG 4 cut(s) 48, 235, 554, 601
AluBI AGCT 1 cut(s) 515
AluI AGCT 1 cut(s) 515
Alw21I GWGCWC 1 cut(s) 439
Alw44I GTGCAC 1 cut(s) 435
AoxI GGCC 2 cut(s) 51, 625
ApaLI GTGCAC 1 cut(s) 435
ApeKI GCWGC 1 cut(s) 512
ApoI RAATTY 4 cut(s) 62, 178, 248, 549
AspS9I GGNCC 2 cut(s) 605, 625
AsuC2I CCSGG 1 cut(s) 184
AsuHPI GGTGA 1 cut(s) 248
AvaII GGWCC 1 cut(s) 605
BaeGI GKGCMC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 706
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 1 cut(s) 524
BciT130I CCWGG 4 cut(s) 50, 237, 556, 603
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 184
BfaI CTAG 2 cut(s) 368, 401
BisI GCNGC 2 cut(s) 69, 513
BlsI GCNGC 2 cut(s) 70, 514
Bme1390I CCNGG 5 cut(s) 50, 184, 237, 556, 603
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 605, 625
BmiI GGNNCC 1 cut(s) 626
BmrFI CCNGG 5 cut(s) 50, 184, 237, 556, 603
BmsI GCATC 4 cut(s) 532, 568, 722, 734
BpiI GAAGAC 1 cut(s) 706
BplI GAGNNNNNCTC 2 cut(s) 318, 350
BpmI CTGGAG 1 cut(s) 32
BpuMI CCSGG 1 cut(s) 184
BsaJI CCNNGG 5 cut(s) 235, 236, 317, 554, 704
Bsc4I CCNNNNNNNGG 3 cut(s) 49, 622, 623
Bse1I ACTGG 2 cut(s) 302, 513
Bse3DI GCAATG 1 cut(s) 645
BseBI CCWGG 4 cut(s) 50, 237, 556, 603
BseDI CCNNGG 5 cut(s) 235, 236, 317, 554, 704
BseGI GGATG 4 cut(s) 235, 351, 526, 547
BseLI CCNNNNNNNGG 3 cut(s) 49, 622, 623
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 302, 513
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 1 cut(s) 524
BshFI GGCC 2 cut(s) 53, 627
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 1 cut(s) 184
BslI CCNNNNNNNGG 3 cut(s) 49, 622, 623
BsnI GGCC 2 cut(s) 53, 627
Bsp1286I GDGCHC 2 cut(s) 336, 439
Bsp143I GATC 1 cut(s) 253
BspACI CCGC 3 cut(s) 39, 57, 69
BspANI GGCC 2 cut(s) 53, 627
BspCNI CTCAG 1 cut(s) 141
BspLI GGNNCC 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 645
BsrI ACTGG 2 cut(s) 302, 513
BssECI CCNNGG 5 cut(s) 235, 236, 317, 554, 704
BssMI GATC 1 cut(s) 253
BssT1I CCWWGG 1 cut(s) 317
Bst2UI CCWGG 4 cut(s) 50, 237, 556, 603
Bst4CI ACNGT 4 cut(s) 123, 225, 434, 746
BstDEI CTNAG 2 cut(s) 149, 416
BstF5I GGATG 4 cut(s) 235, 351, 526, 547
BstKTI GATC 1 cut(s) 256
BstMBI GATC 1 cut(s) 253
BstMWI GCNNNNNNNGC 2 cut(s) 65, 598
BstNI CCWGG 4 cut(s) 50, 237, 556, 603
BstNSI RCATGY 1 cut(s) 140
BstSCI CCNGG 5 cut(s) 48, 182, 235, 554, 601
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 524
BstV2I GAAGAC 1 cut(s) 706
BsuRI GGCC 2 cut(s) 53, 627
BtsCI GGATG 4 cut(s) 235, 351, 526, 547
BtsI GCAGTG 1 cut(s) 587
BtsIMutI CAGTG 1 cut(s) 587
Cfr13I GGNCC 2 cut(s) 605, 625
Csp6I GTAC 6 cut(s) 134, 187, 365, 392, 467, 694
CviAII CATG 5 cut(s) 101, 137, 198, 211, 571
CviJI RGCY 9 cut(s) 53, 85, 105, 160, 301, 334, 488, 515, 627
CviKI_1 RGCY 9 cut(s) 53, 85, 105, 160, 301, 334, 488, 515, 627
CviQI GTAC 6 cut(s) 134, 187, 365, 392, 467, 694
DdeI CTNAG 2 cut(s) 149, 416
DpnI GATC 1 cut(s) 255
DpnII GATC 1 cut(s) 253
DraI TTTAAA 1 cut(s) 504
EciI GGCGGA 2 cut(s) 28, 46
Eco130I CCWWGG 1 cut(s) 317
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 605
Eco57I CTGAAG 3 cut(s) 158, 516, 557
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 4 cut(s) 48, 235, 554, 601
EcoT14I CCWWGG 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 5 cut(s) 104, 140, 201, 214, 574
FaiI YATR 8 cut(s) 102, 138, 199, 212, 283, 388, 572, 610
FatI CATG 5 cut(s) 100, 136, 197, 210, 570
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 69, 513
FokI GGATG 4 cut(s) 242, 338, 533, 554
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 2 cut(s) 69, 513
FspBI CTAG 2 cut(s) 368, 401
GluI GCNGC 2 cut(s) 69, 513
GsuI CTGGAG 1 cut(s) 32
HaeIII GGCC 2 cut(s) 53, 627
HapII CCGG 1 cut(s) 184
Hin1II CATG 5 cut(s) 104, 140, 201, 214, 574
HinfI GANTC 1 cut(s) 7
HpaII CCGG 1 cut(s) 184
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 3 cut(s) 208, 392, 437
Hpy188I TCNGA 7 cut(s) 150, 177, 229, 537, 708, 742, 760
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 3 cut(s) 208, 392, 437
HpyAV CCTTC 3 cut(s) 171, 532, 703
HpyCH4III ACNGT 4 cut(s) 123, 225, 434, 746
HpyCH4V TGCA 3 cut(s) 437, 545, 683
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 598
HpyF3I CTNAG 2 cut(s) 149, 416
Hsp92II CATG 5 cut(s) 104, 140, 201, 214, 574
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 1 cut(s) 253
Lsp1109I GCAGC 1 cut(s) 524
LweI GCATC 4 cut(s) 532, 568, 722, 734
MaeI CTAG 2 cut(s) 368, 401
MaeIII GTNAC 1 cut(s) 20
MalI GATC 1 cut(s) 255
MboI GATC 1 cut(s) 253
MboII GAAGA 2 cut(s) 574, 711
MhlI GDGCHC 2 cut(s) 336, 439
MluCI AATT 7 cut(s) 62, 91, 178, 248, 549, 576, 596
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 6 cut(s) 40, 56, 64, 96, 405, 714
MseI TTAA 3 cut(s) 75, 156, 503
MslI CAYNNNNRTG 2 cut(s) 482, 688
MspI CCGG 1 cut(s) 184
MspR9I CCNGG 5 cut(s) 50, 184, 237, 556, 603
MvaI CCWGG 4 cut(s) 50, 237, 556, 603
MwoI GCNNNNNNNGC 2 cut(s) 65, 598
NciI CCSGG 1 cut(s) 184
NdeII GATC 1 cut(s) 253
NlaIII CATG 5 cut(s) 104, 140, 201, 214, 574
NlaIV GGNNCC 1 cut(s) 626
NmuCI GTSAC 1 cut(s) 20
NspI RCATGY 1 cut(s) 140
PasI CCCWGGG 1 cut(s) 236
PceI AGGCCT 1 cut(s) 53
PkrI GCNGC 2 cut(s) 70, 514
Psp6I CCWGG 4 cut(s) 48, 235, 554, 601
PspGI CCWGG 4 cut(s) 48, 235, 554, 601
PspN4I GGNNCC 1 cut(s) 626
PspPI GGNCC 2 cut(s) 605, 625
RsaI GTAC 6 cut(s) 135, 188, 366, 393, 468, 695
RsaNI GTAC 6 cut(s) 134, 187, 365, 392, 467, 694
RseI CAYNNNNRTG 2 cut(s) 482, 688
SaqAI TTAA 3 cut(s) 75, 156, 503
SatI GCNGC 2 cut(s) 69, 513
Sau3AI GATC 1 cut(s) 253
Sau96I GGNCC 2 cut(s) 605, 625
ScrFI CCNGG 5 cut(s) 50, 184, 237, 556, 603
SduI GDGCHC 2 cut(s) 336, 439
SetI ASST 8 cut(s) 48, 261, 363, 397, 472, 517, 607, 706
SfaNI GCATC 4 cut(s) 532, 568, 722, 734
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 2 cut(s) 482, 688
Sse9I AATT 7 cut(s) 62, 91, 178, 248, 549, 576, 596
SseBI AGGCCT 1 cut(s) 53
SsiI CCGC 3 cut(s) 39, 57, 69
SspMI CTAG 2 cut(s) 368, 401
StuI AGGCCT 1 cut(s) 53
StyD4I CCNGG 5 cut(s) 48, 182, 235, 554, 601
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 4 cut(s) 123, 225, 434, 746
TasI AATT 7 cut(s) 62, 91, 178, 248, 549, 576, 596
TatI WGTACW 2 cut(s) 133, 364
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 3 cut(s) 75, 156, 503
Tru9I TTAA 3 cut(s) 75, 156, 503
TscAI CASTG 1 cut(s) 594
TseFI GTSAC 1 cut(s) 20
TseI GCWGC 1 cut(s) 512
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 1 cut(s) 399
TspRI CASTG 1 cut(s) 594
VneI GTGCAC 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 4 cut(s) 62, 178, 248, 549
XceI RCATGY 1 cut(s) 140
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XspI CTAG 2 cut(s) 368, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.