Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0004508

Physical Location & Seq

Genomic Coordinates Reverse (-)

12407923 .. 12413430

Gene Structure 5508 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2118 bp

ATGATTTCTCATTTGGAAAGAATTTCTCGAGGTATAGATTCTCAGAGAGGTTTAACATGCCAAGTTTTAAACTTGAGGTCGTCGCATTTTCGGAAGCTCAACATGATGGGGGAGTTCATGCGGATACACACCCTTAACCTTGATTTTAGTACTAAGCTCACTAGTTTTCAGGAGGATTGTTTTACTTGCATGCCCAACCTAAAGTTTCTTTCTCTGTGTGAGACAAGAATTTCAAATCTCTGGACAACCATCGCTGCACTTTCTAAACTCCCTTCTTTGGTTGAACTTCGATTTCAGAATTGGTCGGGCTGCAATAATGTTGGCCCTTTTTCTGCATCATCTAGTCGGAAGCCTGATGACAAAACTGATTCCAATCTGCCAAATAGTGTTCATTATGGTGGGCGACCATCCATGTGTGTTGGGGAACCAACAGATTACAATTCAAGCATTGAAGAGGTGCTACGGAATTTGTTTGTAGTGGGTAATATGGGTGTAAATGATGATGAACAAAACATGGTTGAAGATTCATCAGATGATAGTGAACTAGACTCACCGAATCCCTTGCAAGAACATGGTTCTGTAGGGCTATTATCAAATGTTTTCTCAAGCTGGAATAGACAGGCCGATCCACAAAATGAGAATCAAAATGAAGAGGAGTCCTTGCAGGGTACCTTCACAAAGCATATTGGAGACATTGAATTGAAATATATTTCTTATCATGCTTCACCAATATGTTTCGAGAAACATTATAGAGAGTACATGATAGCCTCATTACCTCATTTGAGAAGTTTGGATAACTTGCCTATTGGAAAGATTGATCAGGAAAGGGCGAGGATTAAATTTTCAGAGTGTTTTGAGAATCTACCTTATCAGAGGAAGCATAAAGAGAATATTGTCCACATGTTACAGAAGCGTGAAATAAGAGCAAGCCAAACTCATGTGCAAAGTCCTGGTCAGAAATCATCAAGTCTCTATGGAAAATCTCAACACTTCTATACGAGGTCTCTTTGTGCTGCTAAAATGGGATCTTCTGCCTGGCCTTTCTTGCACCCGCTATCGGTTTCAGGCAGAAACTTGGGAGGTGAAAGTAGGAGCTTTCGCCCTAGGCAGTTTGAGTATCATCCATCTGACTCTAGTCTAATGGTCTTTGGAACATTAGATGGTGAAGTAGTTGTAGTCAACCATGAGAATGAAAAAATTGTCGGTTATATCCCATCATCAGGAGCAATGAATAGTGTCCTGGGACTCTGTTGGCTCAAGAAGTATCCCTCTAAGCTAATAGCTGGGTCAGATAACGGTTCATTGAAATTATATGATATCCATCATACACGATCAATGGTAAGGGGCATCTACCATAGTGCTGGTTCTGTGACTTTTGACGAGTTTGACCAGTTGACTTCTGTTCATGTTAACTCCACGGATGAACTATTTCTTGCTAGTGGATACTCAAGAGATGTTTCTTTGTATGACATAAGTAGTGGAAGACGCTTACAGGTGTTTACTGGTATGCATCGGGAGCATATTAATGTACTGAAGTTTGCAAACCATTCCCCATCTCTTTTTGCGACTTCTTCATTTGATAAGGATGTCAAGATGTGGGATCTCAGACAGAAGCCTATACATCCTTGCTATACTTCTTCAAGCCCTAGGGGTAATGTGATGGTTTGCTTTTCTCCAGATGATCATTATCTTTTAGTGTCAGCTGTTGACAATAAGGTTCGACAACTTCTGGCAGTTGATGGGAGACTTCACCTGAATTTTGAGATAGCCTCTACAGGAAGCTCTCAGAATTACACCCGATCCTATTACATGAATGGAAGGGATTATATCATCAGCGGGAGTTGTGATGAGCATGTAGTCCGCATATGCTGTGCTCAAACTGGGAGGCGGCTGAGGGATATATCTTTGGAGGGACGAGGGGGATCTGGAAGTTCTATGTTTGTCCAATCTTTAAGGGGAGATCCCTTCAGAGAATTCAACATGAGCATCTTGGGAACATATATGCGTCCAAGCTCGAGGTCTGAAATTGTCAAGGTCAACATGCTAGCATCCAGTAACTATGCCAAGGAACACCTTGATGGTCAGCAGCCTTGCCCAACCAATAATATGGGAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006293 GO:0006294 GO:0006296 GO:0006325 GO:0006464 GO:0006508 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010390 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016579 GO:0019538 GO:0022607 GO:0030054 GO:0031334 GO:0031461 GO:0031465 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0033522 GO:0033554 GO:0033683 GO:0034622 GO:0034641 GO:0034644 GO:0035518 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043687 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051276 GO:0051716 GO:0051865 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070646 GO:0070647 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0080008 GO:0090304 GO:0090305 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

706

Amino Acids

79.44

Weight (kDa)

6.98

Isoelectric Point (pI)

53.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHU1_N	PF20919	16 - 281	3.1e-52	DWD hypersensitive to UV-B 1, N-terminal
WD40_Prp19	PF24814	371 - 574	1.1e-11	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	376 - 538	1.8e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	383 - 576	4.9e-13	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	384 - 620	1e-17	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	389 - 574	1.4e-11	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	414 - 563	6e-06	RIG second beta-propeller
WD40_CDC20-Fz	PF24807	461 - 574	1.4e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	465 - 617	5.4e-12	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004508)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34280
fragaria_vesca	FvH4_2g04670 FvH4_2g04670
malus_domestica	MD05G1093800.v1.1
prunus_persica	Prupe.8G138700_v2.0.a1 Prupe.8G138700_v2.0.a1
pyrus_communis	pycom05g09290
rosa_chinensis	RchiOBHm_Chr6g0254291 RchiOBHm_Chr6g0254331 RchiOBHm_Chr6g0254371
rosa_laevigata	RLG00000014132 RLG00000014922 RLG00000014929
rosa_multiflora	Rmu_sc0012713.1_g000001 Rmu_sc0012713.1_g000002 Rmu_sc0027609.1_g000001 Rmu_ssc0000412.1_g000017
rosa_roxburghii	Rroxscaffold_7G00210950
rosa_rugosa	Rorug05G0551700 Rorug05G0551800 Rorug05G0551800
rosa_samantha	Rh6AG068100 Rh6AG068600 Rh6BG061000 Rh6BG061300 Rh6CG060800 Rh6CG061200 Rh6DG057900 Rh6DG058100 Rh6DG058500
rosa_wichuraiana	Rw6G005970 Rw6G006010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	668
AccB1I	GGYRCC	1 cut(s)	668
AciI	CCGC	5 cut(s)	121, 1052, 1836, 1861, 1888
AclWI	GGATC	6 cut(s)	620, 1033, 1608, 1794, 1930, 1955
AcsI	RAATTY	6 cut(s)	21, 228, 466, 839, 1756, 1973
AcuI	CTGAAG	2 cut(s)	1553, 1951
AfaI	GTAC	4 cut(s)	151, 670, 758, 1530
AfiI	CCNNNNNNNGG	3 cut(s)	277, 1057, 1220
AflIII	ACRYGT	1 cut(s)	900
AhlI	ACTAGT	1 cut(s)	161
AjnI	CCWGG	3 cut(s)	949, 1034, 1239
AluBI	AGCT	9 cut(s)	97, 157, 609, 1095, 1276, 1283, 1703, 1782, 2013
AluI	AGCT	9 cut(s)	97, 157, 609, 1095, 1276, 1283, 1703, 1782, 2013
Alw21I	GWGCWC	1 cut(s)	1876
Alw26I	GTCTC	5 cut(s)	215, 684, 974, 1008, 1738
AlwI	GGATC	6 cut(s)	620, 1033, 1608, 1794, 1930, 1955
Ama87I	CYCGRG	2 cut(s)	27, 2014
AoxI	GGCC	3 cut(s)	322, 621, 1037
ApeKI	GCWGC	4 cut(s)	254, 309, 1013, 2086
ApoI	RAATTY	6 cut(s)	21, 228, 466, 839, 1756, 1973
ArsI	GACNNNNNNTTYG	2 cut(s)	937, 969
AseI	ATTAAT	1 cut(s)	1524
Asp700I	GAANNNNTTC	1 cut(s)	1428
Asp718I	GGTACC	1 cut(s)	668
AspA2I	CCTAGG	2 cut(s)	1103, 1646
AspS9I	GGNCC	1 cut(s)	323
AsuHPI	GGTGA	5 cut(s)	543, 717, 1094, 1175, 1742
AsuNHI	GCTAGC	1 cut(s)	2044
AvaI	CYCGRG	2 cut(s)	27, 2014
AvrII	CCTAGG	2 cut(s)	1103, 1646
BanI	GGYRCC	1 cut(s)	668
BarI	GAAGNNNNNNTAC	2 cut(s)	444, 476
BbsI	GAAGAC	1 cut(s)	1489
Bbv12I	GWGCWC	1 cut(s)	1876
BbvCI	CCTCAGC	1 cut(s)	1892
BbvI	GCAGC	4 cut(s)	241, 296, 1000, 2098
BcgI	CGANNNNNNTGC	2 cut(s)	544, 578
BciT130I	CCWGG	3 cut(s)	951, 1036, 1241
BciVI	GTATCC	3 cut(s)	117, 1275, 1436
BclI	TGATCA	2 cut(s)	817, 1681
BcoDI	GTCTC	5 cut(s)	215, 684, 974, 1008, 1738
BcuI	ACTAGT	1 cut(s)	161
BfaI	CTAG	8 cut(s)	162, 342, 545, 1104, 1134, 1437, 1647, 2045
BfmI	CTRYAG	2 cut(s)	579, 1773
BfuI	GTATCC	3 cut(s)	117, 1275, 1436
BisI	GCNGC	5 cut(s)	255, 310, 1014, 1889, 2087
BlnI	CCTAGG	2 cut(s)	1103, 1646
BlsI	GCNGC	5 cut(s)	256, 311, 1015, 1890, 2088
BmcAI	AGTACT	1 cut(s)	151
Bme1390I	CCNGG	3 cut(s)	951, 1036, 1241
BmeT110I	CYCGRG	2 cut(s)	27, 2014
BmgT120I	GGNCC	1 cut(s)	323
BmiI	GGNNCC	2 cut(s)	426, 670
BmrFI	CCNGG	3 cut(s)	951, 1036, 1241
BmrI	ACTGGG	1 cut(s)	1890
BmsI	GCATC	5 cut(s)	344, 1356, 1519, 1995, 2057
BmtI	GCTAGC	1 cut(s)	2048
BmuI	ACTGGG	1 cut(s)	1890
BoxI	GACNNNNGTC	1 cut(s)	1134
BpiI	GAAGAC	1 cut(s)	1489
BplI	GAGNNNNNCTC	2 cut(s)	1754, 1786
BpmI	CTGGAG	1 cut(s)	1659
Bpu10I	CCTNAGC	1 cut(s)	1892
BpuEI	CTTGAG	4 cut(s)	94, 589, 1241, 1432
BsaBI	GATNNNNATC	3 cut(s)	372, 1320, 1686
BsaI	GGTCTC	1 cut(s)	1008
BsaJI	CCNNGG	5 cut(s)	1103, 1240, 1416, 1646, 2064
Bsc4I	CCNNNNNNNGG	3 cut(s)	277, 1057, 1220
Bse1I	ACTGG	4 cut(s)	1390, 1507, 1885, 2052
Bse3DI	GCAATG	1 cut(s)	1233
Bse8I	GATNNNNATC	3 cut(s)	372, 1320, 1686
BseBI	CCWGG	3 cut(s)	951, 1036, 1241
BseDI	CCNNGG	5 cut(s)	1103, 1240, 1416, 1646, 2064
BseGI	GGATG	6 cut(s)	407, 1120, 1426, 1591, 1621, 2048
BseJI	GATNNNNATC	3 cut(s)	372, 1320, 1686
BseLI	CCNNNNNNNGG	3 cut(s)	277, 1057, 1220
BseMI	GCAATG	1 cut(s)	1233
BseMII	CTCAG	4 cut(s)	56, 1618, 1799, 1883
BseNI	ACTGG	4 cut(s)	1390, 1507, 1885, 2052
BseRI	GAGGAG	1 cut(s)	668
BseXI	GCAGC	4 cut(s)	241, 296, 1000, 2098
BseYI	CCCAGC	1 cut(s)	1283
BsgI	GTGCAG	1 cut(s)	240
BshFI	GGCC	3 cut(s)	324, 623, 1039
BshNI	GGYRCC	1 cut(s)	668
BsiHKAI	GWGCWC	1 cut(s)	1876
BsiHKCI	CYCGRG	2 cut(s)	27, 2014
BslFI	GGGAC	2 cut(s)	1257, 1926
BslI	CCNNNNNNNGG	3 cut(s)	277, 1057, 1220
BsmAI	GTCTC	5 cut(s)	215, 684, 974, 1008, 1738
BsmFI	GGGAC	2 cut(s)	1257, 1926
BsnI	GGCC	3 cut(s)	324, 623, 1039
Bso31I	GGTCTC	1 cut(s)	1008
BsoBI	CYCGRG	2 cut(s)	27, 2014
Bsp1286I	GDGCHC	1 cut(s)	1876
Bsp143I	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
BspACI	CCGC	5 cut(s)	121, 1052, 1836, 1861, 1888
BspANI	GGCC	3 cut(s)	324, 623, 1039
BspCNI	CTCAG	4 cut(s)	55, 1617, 1798, 1884
BspLI	GGNNCC	2 cut(s)	426, 670
BspOI	GCTAGC	1 cut(s)	2048
BspPI	GGATC	6 cut(s)	620, 1033, 1608, 1794, 1930, 1955
BspT107I	GGYRCC	1 cut(s)	668
BspTNI	GGTCTC	1 cut(s)	1008
BsrDI	GCAATG	1 cut(s)	1233
BsrI	ACTGG	4 cut(s)	1390, 1507, 1885, 2052
BssECI	CCNNGG	5 cut(s)	1103, 1240, 1416, 1646, 2064
BssMI	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
BssT1I	CCWWGG	3 cut(s)	1103, 1646, 2064
Bst2UI	CCWGG	3 cut(s)	951, 1036, 1241
Bst4CI	ACNGT	1 cut(s)	1298
Bst6I	CTCTTC	2 cut(s)	447, 645
BstC8I	GCNNGC	3 cut(s)	191, 928, 2046
BstDEI	CTNAG	6 cut(s)	42, 153, 1272, 1604, 1785, 1892
BstDSI	CCRYGG	1 cut(s)	1416
BstF5I	GGATG	6 cut(s)	407, 1120, 1426, 1591, 1621, 2048
BstKTI	GATC	9 cut(s)	628, 820, 1028, 1334, 1603, 1684, 1802, 1925, 1963
BstMAI	GTCTC	5 cut(s)	215, 684, 974, 1008, 1738
BstMBI	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
BstMWI	GCNNNNNNNGC	2 cut(s)	1045, 1516
BstNI	CCWGG	3 cut(s)	951, 1036, 1241
BstNSI	RCATGY	5 cut(s)	60, 193, 904, 1856, 2044
BstPAI	GACNNNNGTC	1 cut(s)	1134
BstSCI	CCNGG	3 cut(s)	949, 1034, 1239
BstSFI	CTRYAG	2 cut(s)	579, 1773
BstV1I	GCAGC	4 cut(s)	241, 296, 1000, 2098
BstV2I	GAAGAC	1 cut(s)	1489
BstX2I	RGATCY	4 cut(s)	1025, 1600, 1922, 1960
BstXI	CCANNNNNNTGG	2 cut(s)	1361, 2107
BstYI	RGATCY	4 cut(s)	1025, 1600, 1922, 1960
BsuI	GTATCC	3 cut(s)	117, 1275, 1436
BsuRI	GGCC	3 cut(s)	324, 623, 1039
BtgI	CCRYGG	1 cut(s)	1416
BtgZI	GCGATG	1 cut(s)	235
BtsCI	GGATG	6 cut(s)	407, 1120, 1426, 1591, 1621, 2048
Cac8I	GCNNGC	3 cut(s)	191, 928, 2046
Cfr13I	GGNCC	1 cut(s)	323
CseI	GACGC	2 cut(s)	1494, 1994
Csp6I	GTAC	4 cut(s)	150, 669, 757, 1529
CviQI	GTAC	4 cut(s)	150, 669, 757, 1529
DdeI	CTNAG	6 cut(s)	42, 153, 1272, 1604, 1785, 1892
DpnI	GATC	9 cut(s)	627, 819, 1027, 1333, 1602, 1683, 1801, 1924, 1962
DpnII	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
DraI	TTTAAA	1 cut(s)	69
Eam1104I	CTCTTC	2 cut(s)	447, 645
EarI	CTCTTC	2 cut(s)	447, 645
Eco130I	CCWWGG	3 cut(s)	1103, 1646, 2064
Eco31I	GGTCTC	1 cut(s)	1008
Eco32I	GATATC	1 cut(s)	1318
Eco57I	CTGAAG	2 cut(s)	1553, 1951
Eco88I	CYCGRG	2 cut(s)	27, 2014
EcoRI	GAATTC	1 cut(s)	1973
EcoRII	CCWGG	3 cut(s)	949, 1034, 1239
EcoRV	GATATC	1 cut(s)	1318
EcoT14I	CCWWGG	3 cut(s)	1103, 1646, 2064
EcoT22I	ATGCAT	1 cut(s)	1512
ErhI	CCWWGG	3 cut(s)	1103, 1646, 2064
FaqI	GGGAC	2 cut(s)	1257, 1926
FauI	CCCGC	2 cut(s)	1059, 1829
FauNDI	CATATG	1 cut(s)	1865
FbaI	TGATCA	2 cut(s)	817, 1681
Fnu4HI	GCNGC	5 cut(s)	255, 310, 1014, 1889, 2087
FokI	GGATG	6 cut(s)	394, 1107, 1433, 1598, 1608, 2035
Fsp4HI	GCNGC	5 cut(s)	255, 310, 1014, 1889, 2087
FspBI	CTAG	8 cut(s)	162, 342, 545, 1104, 1134, 1437, 1647, 2045
GluI	GCNGC	5 cut(s)	255, 310, 1014, 1889, 2087
GsaI	CCCAGC	1 cut(s)	1287
GsuI	CTGGAG	1 cut(s)	1659
HaeIII	GGCC	3 cut(s)	324, 623, 1039
HgaI	GACGC	2 cut(s)	1494, 1994
HincII	GTYRAC	5 cut(s)	1180, 1395, 1411, 1708, 2038
HindII	GTYRAC	5 cut(s)	1180, 1395, 1411, 1708, 2038
HpaI	GTTAAC	1 cut(s)	1411
HphI	GGTGA	5 cut(s)	543, 717, 1094, 1175, 1742
Hpy166II	GTNNAC	8 cut(s)	542, 898, 1180, 1395, 1411, 1500, 1708, 2038
Hpy8I	GTNNAC	8 cut(s)	542, 898, 1180, 1395, 1411, 1500, 1708, 2038
Hpy99I	CGWCG	1 cut(s)	85
HpyAV	CCTTC	4 cut(s)	282, 682, 1812, 1975
HpyCH4III	ACNGT	1 cut(s)	1298
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1045, 1516
HpyF3I	CTNAG	6 cut(s)	42, 153, 1272, 1604, 1785, 1892
KpnI	GGTACC	1 cut(s)	672
Ksp22I	TGATCA	2 cut(s)	817, 1681
KspAI	GTTAAC	1 cut(s)	1411
Kzo9I	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
LmnI	GCTCC	3 cut(s)	1092, 1223, 1516
Lsp1109I	GCAGC	4 cut(s)	241, 296, 1000, 2098
LweI	GCATC	5 cut(s)	344, 1356, 1519, 1995, 2057
MaeI	CTAG	8 cut(s)	162, 342, 545, 1104, 1134, 1437, 1647, 2045
MaeIII	GTNAC	3 cut(s)	903, 1369, 2054
MalI	GATC	9 cut(s)	627, 819, 1027, 1333, 1602, 1683, 1801, 1924, 1962
MboI	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
MboII	GAAGA	7 cut(s)	464, 533, 662, 1020, 1494, 1563, 1629
MflI	RGATCY	4 cut(s)	1025, 1600, 1922, 1960
MhlI	GDGCHC	1 cut(s)	1876
MlyI	GAGTC	4 cut(s)	542, 665, 1124, 1239
MmeI	TCCRAC	1 cut(s)	326
Mph1103I	ATGCAT	1 cut(s)	1512
MroXI	GAANNNNTTC	1 cut(s)	1428
MseI	TTAA	7 cut(s)	53, 68, 135, 837, 1410, 1524, 1952
MslI	CAYNNNNRTG	6 cut(s)	396, 412, 730, 1188, 1524, 2076
MspA1I	CMGCKG	2 cut(s)	1703, 1836
MspR9I	CCNGG	3 cut(s)	951, 1036, 1241
MvaI	CCWGG	3 cut(s)	951, 1036, 1241
MwoI	GCNNNNNNNGC	2 cut(s)	1045, 1516
NdeI	CATATG	1 cut(s)	1865
NdeII	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
NheI	GCTAGC	1 cut(s)	2044
NlaIV	GGNNCC	2 cut(s)	426, 670
NmuCI	GTSAC	1 cut(s)	1369
NsiI	ATGCAT	1 cut(s)	1512
NspI	RCATGY	5 cut(s)	60, 193, 904, 1856, 2044
PaeI	GCATGC	1 cut(s)	193
PaeR7I	CTCGAG	2 cut(s)	27, 2014
PciI	ACATGT	1 cut(s)	900
PdmI	GAANNNNTTC	1 cut(s)	1428
PfeI	GAWTC	6 cut(s)	38, 368, 524, 556, 640, 859
PkrI	GCNGC	5 cut(s)	256, 311, 1015, 1890, 2088
PleI	GAGTC	4 cut(s)	542, 664, 1124, 1239
PpsI	GAGTC	4 cut(s)	542, 664, 1124, 1239
PscI	ACATGT	1 cut(s)	900
PshAI	GACNNNNGTC	1 cut(s)	1134
PshBI	ATTAAT	1 cut(s)	1524
Psp6I	CCWGG	3 cut(s)	949, 1034, 1239
PspFI	CCCAGC	1 cut(s)	1283
PspGI	CCWGG	3 cut(s)	949, 1034, 1239
PspN4I	GGNNCC	2 cut(s)	426, 670
PspPI	GGNCC	1 cut(s)	323
PspXI	VCTCGAGB	1 cut(s)	2014
PsuI	RGATCY	4 cut(s)	1025, 1600, 1922, 1960
PvuII	CAGCTG	1 cut(s)	1703
RsaI	GTAC	4 cut(s)	151, 670, 758, 1530
RsaNI	GTAC	4 cut(s)	150, 669, 757, 1529
RseI	CAYNNNNRTG	6 cut(s)	396, 412, 730, 1188, 1524, 2076
SaqAI	TTAA	7 cut(s)	53, 68, 135, 837, 1410, 1524, 1952
SatI	GCNGC	5 cut(s)	255, 310, 1014, 1889, 2087
Sau3AI	GATC	9 cut(s)	625, 817, 1025, 1331, 1600, 1681, 1799, 1922, 1960
Sau96I	GGNCC	1 cut(s)	323
ScaI	AGTACT	1 cut(s)	151
SchI	GAGTC	4 cut(s)	542, 665, 1124, 1239
ScrFI	CCNGG	3 cut(s)	951, 1036, 1241
SduI	GDGCHC	1 cut(s)	1876
SfaNI	GCATC	5 cut(s)	344, 1356, 1519, 1995, 2057
SfcI	CTRYAG	2 cut(s)	579, 1773
Sfr274I	CTCGAG	2 cut(s)	27, 2014
SlaI	CTCGAG	2 cut(s)	27, 2014
SmiMI	CAYNNNNRTG	6 cut(s)	396, 412, 730, 1188, 1524, 2076
SmlI	CTYRAG	6 cut(s)	27, 73, 604, 1256, 1447, 2014
SmoI	CTYRAG	6 cut(s)	27, 73, 604, 1256, 1447, 2014
SpeI	ACTAGT	1 cut(s)	161
SphI	GCATGC	1 cut(s)	193
SsiI	CCGC	5 cut(s)	121, 1052, 1836, 1861, 1888
SspI	AATATT	1 cut(s)	892
SspMI	CTAG	8 cut(s)	162, 342, 545, 1104, 1134, 1437, 1647, 2045
StyD4I	CCNGG	3 cut(s)	949, 1034, 1239
StyI	CCWWGG	3 cut(s)	1103, 1646, 2064
TaaI	ACNGT	1 cut(s)	1298
TaqI	TCGA	5 cut(s)	28, 289, 738, 1720, 2015
TatI	WGTACW	3 cut(s)	149, 756, 1528
TauI	GCSGC	1 cut(s)	1891
TfiI	GAWTC	6 cut(s)	38, 368, 524, 556, 640, 859
Tru1I	TTAA	7 cut(s)	53, 68, 135, 837, 1410, 1524, 1952
Tru9I	TTAA	7 cut(s)	53, 68, 135, 837, 1410, 1524, 1952
TseFI	GTSAC	1 cut(s)	1369
TseI	GCWGC	4 cut(s)	254, 309, 1013, 2086
Tsp45I	GTSAC	1 cut(s)	1369
TspGWI	ACGGA	2 cut(s)	478, 1433
VspI	ATTAAT	1 cut(s)	1524
XapI	RAATTY	6 cut(s)	21, 228, 466, 839, 1756, 1973
XceI	RCATGY	5 cut(s)	60, 193, 904, 1856, 2044
XhoI	CTCGAG	2 cut(s)	27, 2014
XmaJI	CCTAGG	2 cut(s)	1103, 1646
XmnI	GAANNNNTTC	1 cut(s)	1428
XspI	CTAG	8 cut(s)	162, 342, 545, 1104, 1134, 1437, 1647, 2045
ZrmI	AGTACT	1 cut(s)	151
Zsp2I	ATGCAT	1 cut(s)	1512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.