pycom05g15050

sodium metabolite cotransporter BASS3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008339
Physical Location & Seq
Forward (+)
18589156 .. 18591959
2804 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1512 bp
ATGTTAGTGCAGTATTCTATAGCATCGAATCATGTTAGTGCACGACTCTGTCTGACGCAGCAAAACACTCCCACCTTTCTTCTCTCTGTTTTTCCAATCGAATTTTATAGAAAGAAAATCCATTCAGTCTATCACTTCATCTCTTCCCAACACCACCCAATTCCTCGCCGGAAACAGCTGTTATCCGGCGGAGAAACCCCCTTTCACCGCCGACCCATTGCCATGTCCACCACTCCCATCTCCTATCCAATAACCTCAAGACCCTTCCTTTCTCTCACACCCGCCACCGCAGCCTCAAAACTCTTTGCGAACCCGAATTACCACACCCAAAGCTCTCTCTTTTCCTCCTCTTCGTCTTCCTCCAGTCATTTCCCCAAAAGGGTATTCGGAAATGGGGTTTCTCTGTCGATAAGAGCTTGCTCGACGTCGCCGTTTGTCGGCAGAGTCGGGTCGCAAAGGAGGGAAGGCAACGCGTCGATGCTGTCGTTTGGGATAAACCCATCAGCTCAAATATCCAAAGGGGCTTCCGACGGCTCGTCGTCTCAGGTCTTATCTGCAATGCTTCCTTTCGTCGTTGCTGCTACCGCCATTGCTGCTCTGGCTCAGCCGTCAACGTTCACTTGGGTATCCAAGGATTTATATGCCCCTGCTCTTGGTGGGATCATGCTGTCAATTGGAATTAAACTTTCGGTTGATGATTTTGCTCTTGCATTCAAAAGACCTTTACCACTCTCTGTTGGGTTTATCGCGCAGTATGTGCTGAAACCGCTACTTGGGGTACTAATTGCAAACGCATTTGGCGTGCCGAGGGTGTTCTACGCTGGCTTTGTTCTCACCGCTTGTGTTGCAGGGGCTCAGCTGTCTAGCTATGCTAGTTTCTTGAGCAAAGGAGATGTGGCCTTGAGCATTCTCCTCACCAGCACCACCACCATTGCTTCCGTGATCGTAACGCCTTTACTAACTGGCCTTTTAATCGGTTCTGTTGTTCCGGTTGATGCTGTTGCCATGTCAAAGTCAATATTGCAGGTGGTTCTTGTTCCGGTCACAATTGGTCTTGTGCTCAATACTTACGCAAAACCAGTTGTCAATTTCCTTCAACCAGTGATGCCAGTTGTTGCTATGATTTGCACTTCATTGTGCATTGGTAGTCCTCTTGCAATTAACCGGAGTCAAATTCTATCCGTAGAGGGTTTCCGGTTGATATTTCCAGTCATAGCATTTCACGCTGTGGCCTTTACACTTGGATACTGGGTATCCAAAACTCCAGGTTTGAGGCAAGAAGAAGAAGTTAGTAGGACACTTTCCTTGTGCACAGGAATGCAGAGTTCCACTTTGGCAGGGCTCCTTGCAAGCCAGTTCCTTGGGAGCAGCCAGGCCGTTCCTCCAGCATGTTCAGTTGTTGCCATGGCCATCATGGGCCTGTTTCTTGCGTCTTTCTGGGGCACCGGCTCTCGAATCAGAGACCTGCTGTCCCTACTAACGCCTCAAACAGGTTCTCCAATGAAGGCGTAG

Protein Analysis

504

Amino Acids

53.34

Weight (kDa)

10.03

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBF PF01758 216 - 394 1.9e-34 Sodium Bile acid symporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1015
AatII GACGTC 1 cut(s) 428
Acc36I ACCTGC 2 cut(s) 1015, 1473
AccB1I GGYRCC 1 cut(s) 1442
AccII CGCG 2 cut(s) 473, 749
AciI CCGC 7 cut(s) 189, 208, 282, 288, 585, 767, 837
AclI AACGTT 1 cut(s) 614
AclWI GGATC 1 cut(s) 668
AcoI YGGCCR 1 cut(s) 1407
AcsI RAATTY 2 cut(s) 101, 1173
AcyI GRCGYC 1 cut(s) 425
AdeI CACNNNGTG 1 cut(s) 1228
AfaI GTAC 1 cut(s) 780
AfiI CCNNNNNNNGG 5 cut(s) 379, 437, 653, 773, 1490
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 2 cut(s) 715, 1097
AhdI GACNNNNNGTC 2 cut(s) 535, 1468
AjnI CCWGG 2 cut(s) 1264, 1371
AjuI GAANNNNNNNTTGG 2 cut(s) 368, 400
AluBI AGCT 6 cut(s) 178, 333, 416, 506, 859, 867
AluI AGCT 6 cut(s) 178, 333, 416, 506, 859, 867
Alw21I GWGCWC 3 cut(s) 43, 1062, 1313
Alw26I GTCTC 2 cut(s) 546, 1455
Alw44I GTGCAC 2 cut(s) 39, 1309
AlwI GGATC 1 cut(s) 668
AoxI GGCC 6 cut(s) 897, 964, 1230, 1374, 1407, 1417
ApaLI GTGCAC 2 cut(s) 39, 1309
ApeKI GCWGC 5 cut(s) 58, 290, 578, 593, 1368
ApoI RAATTY 2 cut(s) 101, 1173
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 1417
AsuHPI GGTGA 3 cut(s) 197, 826, 907
BaeGI GKGCMC 3 cut(s) 43, 1313, 1445
BaeI ACNNNNGTAYC 2 cut(s) 609, 642
BalI TGGCCA 1 cut(s) 1409
BanI GGYRCC 1 cut(s) 1442
BanII GRGCYC 2 cut(s) 856, 1344
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 3 cut(s) 43, 1062, 1313
BbvI GCAGC 5 cut(s) 70, 302, 565, 580, 1380
BccI CCATC 3 cut(s) 245, 508, 1418
BceAI ACGGC 4 cut(s) 415, 547, 592, 1361
BciT130I CCWGG 2 cut(s) 1266, 1373
BciVI GTATCC 3 cut(s) 637, 1238, 1264
BcoDI GTCTC 2 cut(s) 546, 1455
BfaI CTAG 2 cut(s) 864, 873
BfmI CTRYAG 1 cut(s) 18
BfuAI ACCTGC 2 cut(s) 1015, 1473
BfuI GTATCC 3 cut(s) 637, 1238, 1264
BisI GCNGC 5 cut(s) 59, 291, 579, 594, 1369
BlpI GCTNAGC 2 cut(s) 603, 855
BlsI GCNGC 5 cut(s) 60, 292, 580, 595, 1370
Bme1390I CCNGG 2 cut(s) 1266, 1373
BmeRI GACNNNNNGTC 2 cut(s) 535, 1468
BmgT120I GGNCC 1 cut(s) 1417
BmiI GGNNCC 2 cut(s) 1343, 1444
BmrFI CCNGG 2 cut(s) 1266, 1373
BmrI ACTGGG 1 cut(s) 1258
BmsI GCATC 4 cut(s) 32, 468, 985, 1095
BmuI ACTGGG 1 cut(s) 1258
BpiI GAAGAC 1 cut(s) 348
BpmI CTGGAG 3 cut(s) 346, 1248, 1368
Bpu1102I GCTNAGC 2 cut(s) 603, 855
BpuEI CTTGAG 3 cut(s) 241, 901, 922
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 1455
BsaJI CCNNGG 4 cut(s) 630, 806, 1360, 1404
BsaWI WCCGGW 4 cut(s) 988, 1039, 1164, 1194
BsaXI ACNNNNNCTCC 2 cut(s) 1480, 1510
Bsc4I CCNNNNNNNGG 5 cut(s) 379, 437, 653, 773, 1490
Bse118I RCCGGY 1 cut(s) 1445
Bse1I ACTGG 8 cut(s) 363, 967, 1079, 1100, 1109, 1208, 1253, 1354
Bse3DI GCAATG 4 cut(s) 216, 564, 588, 930
BseBI CCWGG 2 cut(s) 1266, 1373
BseDI CCNNGG 4 cut(s) 630, 806, 1360, 1404
BseLI CCNNNNNNNGG 5 cut(s) 379, 437, 653, 773, 1490
BseMI GCAATG 4 cut(s) 216, 564, 588, 930
BseMII CTCAG 3 cut(s) 557, 617, 869
BseNI ACTGG 8 cut(s) 363, 967, 1079, 1100, 1109, 1208, 1253, 1354
BseRI GAGGAG 2 cut(s) 337, 902
BseSI GKGCMC 3 cut(s) 43, 1313, 1445
BseXI GCAGC 5 cut(s) 70, 302, 565, 580, 1380
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 2 cut(s) 473, 749
BshFI GGCC 6 cut(s) 899, 966, 1232, 1376, 1409, 1419
BshNI GGYRCC 1 cut(s) 1442
BsiHKAI GWGCWC 3 cut(s) 43, 1062, 1313
BsiSI CCGG 7 cut(s) 169, 186, 989, 1040, 1165, 1195, 1446
BslFI GGGAC 1 cut(s) 1456
BslI CCNNNNNNNGG 5 cut(s) 379, 437, 653, 773, 1490
BsmAI GTCTC 2 cut(s) 546, 1455
BsmBI CGTCTC 1 cut(s) 546
BsmFI GGGAC 1 cut(s) 1456
BsmI GAATGC 3 cut(s) 710, 906, 1323
BsnI GGCC 6 cut(s) 899, 966, 1232, 1376, 1409, 1419
Bso31I GGTCTC 1 cut(s) 1455
Bsp1286I GDGCHC 6 cut(s) 43, 856, 1062, 1313, 1344, 1445
Bsp143I GATC 2 cut(s) 660, 942
Bsp1720I GCTNAGC 2 cut(s) 603, 855
Bsp19I CCATGG 1 cut(s) 1404
BspACI CCGC 7 cut(s) 189, 208, 282, 288, 585, 767, 837
BspANI GGCC 6 cut(s) 899, 966, 1232, 1376, 1409, 1419
BspCNI CTCAG 3 cut(s) 556, 616, 868
BspFNI CGCG 2 cut(s) 473, 749
BspLI GGNNCC 2 cut(s) 1343, 1444
BspMI ACCTGC 2 cut(s) 1015, 1473
BspPI GGATC 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 1442
BspTNI GGTCTC 1 cut(s) 1455
BsrDI GCAATG 4 cut(s) 216, 564, 588, 930
BsrFI RCCGGY 1 cut(s) 1445
BsrI ACTGG 8 cut(s) 363, 967, 1079, 1100, 1109, 1208, 1253, 1354
BssAI RCCGGY 1 cut(s) 1445
BssECI CCNNGG 4 cut(s) 630, 806, 1360, 1404
BssMI GATC 2 cut(s) 660, 942
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 3 cut(s) 630, 1360, 1404
Bst2UI CCWGG 2 cut(s) 1266, 1373
Bst6I CTCTTC 2 cut(s) 148, 355
BstACI GRCGYC 1 cut(s) 425
BstAPI GCANNNNNTGC 1 cut(s) 757
BstC8I GCNNGC 4 cut(s) 418, 803, 823, 1351
BstDEI CTNAG 3 cut(s) 543, 603, 855
BstDSI CCRYGG 1 cut(s) 1404
BstENI CCTNNNNNAGG 1 cut(s) 1488
BstFNI CGCG 2 cut(s) 473, 749
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 2 cut(s) 663, 945
BstMAI GTCTC 2 cut(s) 546, 1455
BstMBI GATC 2 cut(s) 660, 942
BstMWI GCNNNNNNNGC 8 cut(s) 290, 584, 593, 599, 757, 766, 845, 1223
BstNI CCWGG 2 cut(s) 1266, 1373
BstNSI RCATGY 1 cut(s) 1392
BstSCI CCNGG 2 cut(s) 1264, 1371
BstSFI CTRYAG 1 cut(s) 18
BstSLI GKGCMC 3 cut(s) 43, 1313, 1445
BstUI CGCG 2 cut(s) 473, 749
BstV1I GCAGC 5 cut(s) 70, 302, 565, 580, 1380
BstV2I GAAGAC 1 cut(s) 348
BstXI CCANNNNNNTGG 1 cut(s) 1361
BsuI GTATCC 3 cut(s) 637, 1238, 1264
BsuRI GGCC 6 cut(s) 899, 966, 1232, 1376, 1409, 1419
BtgI CCRYGG 1 cut(s) 1404
BtsIMutI CAGTG 1 cut(s) 1107
BveI ACCTGC 2 cut(s) 1015, 1473
Cac8I GCNNGC 4 cut(s) 418, 803, 823, 1351
CfoI GCGC 1 cut(s) 751
Cfr10I RCCGGY 1 cut(s) 1445
Cfr13I GGNCC 1 cut(s) 1417
CseI GACGC 3 cut(s) 64, 462, 1419
Csp6I GTAC 1 cut(s) 779
CspCI CAANNNNNGTGG 2 cut(s) 913, 948
CviAII CATG 7 cut(s) 32, 223, 664, 1006, 1389, 1405, 1414
CviQI GTAC 1 cut(s) 779
DdeI CTNAG 3 cut(s) 543, 603, 855
DpnI GATC 2 cut(s) 662, 944
DpnII GATC 2 cut(s) 660, 942
DraIII CACNNNGTG 1 cut(s) 1228
DriI GACNNNNNGTC 2 cut(s) 535, 1468
EaeI YGGCCR 1 cut(s) 1407
Eam1104I CTCTTC 2 cut(s) 148, 355
Eam1105I GACNNNNNGTC 2 cut(s) 535, 1468
EarI CTCTTC 2 cut(s) 148, 355
EciI GGCGGA 1 cut(s) 204
Eco130I CCWWGG 3 cut(s) 630, 1360, 1404
Eco24I GRGCYC 2 cut(s) 856, 1344
Eco31I GGTCTC 1 cut(s) 1455
EcoNI CCTNNNNNAGG 1 cut(s) 1488
EcoRII CCWGG 2 cut(s) 1264, 1371
EcoT14I CCWWGG 3 cut(s) 630, 1360, 1404
EcoT38I GRGCYC 2 cut(s) 856, 1344
ErhI CCWWGG 3 cut(s) 630, 1360, 1404
Esp3I CGTCTC 1 cut(s) 546
FaeI CATG 7 cut(s) 35, 226, 667, 1009, 1392, 1408, 1417
FaqI GGGAC 1 cut(s) 1456
FatI CATG 7 cut(s) 31, 222, 663, 1005, 1388, 1404, 1413
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 5 cut(s) 59, 291, 579, 594, 1369
FriOI GRGCYC 2 cut(s) 856, 1344
Fsp4HI GCNGC 5 cut(s) 59, 291, 579, 594, 1369
FspBI CTAG 2 cut(s) 864, 873
GlaI GCGC 1 cut(s) 750
GluI GCNGC 5 cut(s) 59, 291, 579, 594, 1369
GsuI CTGGAG 3 cut(s) 346, 1248, 1368
HaeIII GGCC 6 cut(s) 899, 966, 1232, 1376, 1409, 1419
HapII CCGG 7 cut(s) 169, 186, 989, 1040, 1165, 1195, 1446
HgaI GACGC 3 cut(s) 64, 462, 1419
HhaI GCGC 1 cut(s) 751
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 7 cut(s) 35, 226, 667, 1009, 1392, 1408, 1417
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HinfI GANTC 5 cut(s) 28, 45, 444, 1168, 1455
HpaII CCGG 7 cut(s) 169, 186, 989, 1040, 1165, 1195, 1446
HphI GGTGA 3 cut(s) 197, 826, 907
Hpy166II GTNNAC 5 cut(s) 41, 228, 612, 618, 1311
Hpy188I TCNGA 4 cut(s) 54, 389, 529, 1460
Hpy188III TCNNGA 3 cut(s) 258, 880, 1452
Hpy8I GTNNAC 5 cut(s) 41, 228, 612, 618, 1311
Hpy99I CGWCG 6 cut(s) 427, 430, 478, 533, 541, 575
HpyAV CCTTC 4 cut(s) 274, 458, 1103, 1498
HpyCH4IV ACGT 2 cut(s) 425, 614
HpyF10VI GCNNNNNNNGC 8 cut(s) 290, 584, 593, 599, 757, 766, 845, 1223
HpyF3I CTNAG 3 cut(s) 543, 603, 855
HpySE526I ACGT 2 cut(s) 425, 614
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 7 cut(s) 35, 226, 667, 1009, 1392, 1408, 1417
HspAI GCGC 1 cut(s) 749
Kzo9I GATC 2 cut(s) 660, 942
LmnI GCTCC 2 cut(s) 1347, 1365
Lsp1109I GCAGC 5 cut(s) 70, 302, 565, 580, 1380
LweI GCATC 4 cut(s) 32, 468, 985, 1095
MaeI CTAG 2 cut(s) 864, 873
MaeII ACGT 2 cut(s) 425, 614
MaeIII GTNAC 2 cut(s) 946, 1042
MalI GATC 2 cut(s) 662, 944
MboI GATC 2 cut(s) 660, 942
MboII GAAGA 6 cut(s) 71, 135, 342, 348, 1292, 1295
MfeI CAATTG 2 cut(s) 672, 1047
MhlI GDGCHC 6 cut(s) 43, 856, 1062, 1313, 1344, 1445
MlsI TGGCCA 1 cut(s) 1409
MluI ACGCGT 1 cut(s) 471
MluNI TGGCCA 1 cut(s) 1409
MlyI GAGTC 3 cut(s) 39, 453, 1177
MmeI TCCRAC 1 cut(s) 552
Mox20I TGGCCA 1 cut(s) 1409
MscI TGGCCA 1 cut(s) 1409
MseI TTAA 3 cut(s) 681, 971, 1161
MslI CAYNNNNRTG 3 cut(s) 36, 221, 1316
Msp20I TGGCCA 1 cut(s) 1409
MspA1I CMGCKG 2 cut(s) 178, 859
MspI CCGG 7 cut(s) 169, 186, 989, 1040, 1165, 1195, 1446
MspR9I CCNGG 2 cut(s) 1266, 1373
MunI CAATTG 2 cut(s) 672, 1047
Mva1269I GAATGC 3 cut(s) 710, 906, 1323
MvaI CCWGG 2 cut(s) 1266, 1373
MvnI CGCG 2 cut(s) 473, 749
MwoI GCNNNNNNNGC 8 cut(s) 290, 584, 593, 599, 757, 766, 845, 1223
NcoI CCATGG 1 cut(s) 1404
NdeII GATC 2 cut(s) 660, 942
NlaIII CATG 7 cut(s) 35, 226, 667, 1009, 1392, 1408, 1417
NlaIV GGNNCC 2 cut(s) 1343, 1444
NmeAIII GCCGAG 1 cut(s) 831
NmuCI GTSAC 1 cut(s) 1042
NspI RCATGY 1 cut(s) 1392
PaqCI CACCTGC 1 cut(s) 1015
PcsI WCGNNNNNNNCGW 3 cut(s) 428, 482, 798
PctI GAATGC 3 cut(s) 710, 906, 1323
PfeI GAWTC 2 cut(s) 28, 1455
PflFI GACNNNGTC 1 cut(s) 48
PkrI GCNGC 5 cut(s) 60, 292, 580, 595, 1370
PleI GAGTC 3 cut(s) 39, 452, 1176
PpsI GAGTC 3 cut(s) 39, 452, 1176
Psp1406I AACGTT 1 cut(s) 614
Psp6I CCWGG 2 cut(s) 1264, 1371
PspGI CCWGG 2 cut(s) 1264, 1371
PspN4I GGNNCC 2 cut(s) 1343, 1444
PspPI GGNCC 1 cut(s) 1417
PsrI GAACNNNNNNTAC 2 cut(s) 302, 334
PsyI GACNNNGTC 1 cut(s) 48
PvuII CAGCTG 2 cut(s) 178, 859
RsaI GTAC 1 cut(s) 780
RsaNI GTAC 1 cut(s) 779
RseI CAYNNNNRTG 3 cut(s) 36, 221, 1316
SaqAI TTAA 3 cut(s) 681, 971, 1161
SatI GCNGC 5 cut(s) 59, 291, 579, 594, 1369
Sau3AI GATC 2 cut(s) 660, 942
Sau96I GGNCC 1 cut(s) 1417
SchI GAGTC 3 cut(s) 39, 453, 1177
ScrFI CCNGG 2 cut(s) 1266, 1373
SduI GDGCHC 6 cut(s) 43, 856, 1062, 1313, 1344, 1445
SfaNI GCATC 4 cut(s) 32, 468, 985, 1095
SfcI CTRYAG 1 cut(s) 18
SmiMI CAYNNNNRTG 3 cut(s) 36, 221, 1316
SmlI CTYRAG 3 cut(s) 256, 880, 901
SmoI CTYRAG 3 cut(s) 256, 880, 901
SsiI CCGC 7 cut(s) 189, 208, 282, 288, 585, 767, 837
SspI AATATT 1 cut(s) 1020
SspMI CTAG 2 cut(s) 864, 873
StyD4I CCNGG 2 cut(s) 1264, 1371
StyI CCWWGG 3 cut(s) 630, 1360, 1404
TaiI ACGT 2 cut(s) 428, 617
TaqI TCGA 6 cut(s) 26, 99, 407, 422, 476, 1453
TfiI GAWTC 2 cut(s) 28, 1455
Tru1I TTAA 3 cut(s) 681, 971, 1161
Tru9I TTAA 3 cut(s) 681, 971, 1161
TscAI CASTG 1 cut(s) 1107
TseFI GTSAC 1 cut(s) 1042
TseI GCWGC 5 cut(s) 58, 290, 578, 593, 1368
Tsp45I GTSAC 1 cut(s) 1042
TspDTI ATGAA 2 cut(s) 127, 1122
TspGWI ACGGA 2 cut(s) 928, 1171
TspRI CASTG 1 cut(s) 1107
Tth111I GACNNNGTC 1 cut(s) 48
VneI GTGCAC 2 cut(s) 39, 1309
XagI CCTNNNNNAGG 1 cut(s) 1488
XapI RAATTY 2 cut(s) 101, 1173
XceI RCATGY 1 cut(s) 1392
XcmI CCANNNNNNNNNTGG 2 cut(s) 595, 1411
XspI CTAG 2 cut(s) 864, 873
ZraI GACGTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.