pycom05g15800

Lysine histidine transporter-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0016401
Physical Location & Seq
Forward (+)
19295901 .. 19299745
3845 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2022 bp
ATGGTGAGCTTAAGAAAGCGCAAACGCATGAGACTGGTTTCCGGGGAAAGTAATGCTTTAGTTCCATTTCCCAGTTTTTGGGACAAAGAAATTGCTCCTCAAGATCCAAACCGCATGCCTTCAAATGATGTCATTCAGCCAGAGAGGATGAAAGAAACCAAGAAACCGAGGATAAAGACTTTTGCTAAGAAAAGCCCAACAAAAAAGAAGAAGGATTATCTGCAATATAGATGCAACATGACGGGCTTTGCAGATGCCATGCAATTGTACAAGCCTACGCTTCAACAACGTCAAGACATCTGCGACGAGTTAGCCAAGACACCGTTTTGGAGTCTACTAAAAATGTACATGGATGACATATTGATCGCCACGGATAGAAAGAAGAAGTCCGGTATGGATTTAATAAAGATCATGCAATGCTACAACTCAAAGGAGCAAAAATTTAAGTTTGGAGATAATGAAGCAAAGGGAATAACGAACGATGATGTTGTTCAGATATTTGGTCTGAACAACAGAGGAGTGGAGCTTCCAAACACGGACAAGTCCACGAAACATGGAAAAGAGGACCCCTTCATTGACAAATACTTTAAGCATGCAAAAGTTGTCAAAAGGAAGCAACTAATAGAAGCCTATGAAAAATCACTCCTGGACCAATCTCCTCAAGGTGCAAAAGATACCGCTTCCATAATCTGTGCTCATCTCATCCAATCACTTCTCTTTGCTAATTCTGGCACGTATATTACCTGGAGTTTCTTAAAGATATGTGGGGAGTTGGATGAGATCAGTAAATATAACTGGGCGAAAGGAGTGAAAGATTACCTCCTCAAGATGATAAACAAAGCACAGAAGAAGAAAAAAAATAGAGGAGAAGAACCAACAATTAGTGGCTGCACCGTCTTTGTTTTGTATTGGATATGTCTTCATACAAATGTGGTTAAGACGGTCAGAGGACGAGAAAACATGATTCCGGCAATTGCAAGATGGGATACAAGTATGATACACAAAGCAATCAAAGATGTCCCAGTTGAACAAATCATGATTGATGGTGTCATTCCTTGTGCTCATCCACAAGAAGAATCTTCGAAGAAAAAACAAGAAAAAACAAGAACGAAGAAAAGAAAGCAAAGTGATGTCATTCCTTATGCTCAACCTGAAGATGATAAATTGGATGACCAGTCCCACCAAAAAGAGGAAGCAAACAAAGATGAGATGGCAGAGGTTGATAAAAATTTGAAGAAGAAAAAGAAGAAACAGAAAAGGAAGCAAAATGATGAAGAACATGAAGGCATCAATATAAATGAAGCTCAAGCACAGTTCGATCAACTGTACAAAGAGGAAATGAGCAAACTCGATGCCATTCATCAGAGATACATCATTGGCATGGATGGAACTTTAAGTGTAGATCTTTTCAATGAATTCAAGAGTGCTAGGAGCATTGATCAGAAAAGGTACCTAACCTTCAAGACGATGATGACATATGCACTGGACTCTTCAAACAAAGAGTGCATAGCACTCAGGGAATCAAATAAAATGCTAAACCATGAAATGAAAAAGATTCTAAGACAAGTGGAGCTTGACAAGAAGAAAATGAATGCACTAAATGCTGATTTATTATCATTGCAGACAAAAAACAGTGCAGCTTCTCTAAAAATCAATGAACAGCTAAATGAGATTGGGGCTTTGAAGAAGAAGTTGGAAGAAAAGGAAGCAAAAATTCCAGGTTTGAATGCTTCTGTACACGAACTTCAGGAAGAGTGGAATCAAACTGCTAGTGATTCCAAAGTTCAATCTCCATGGGGTGATGAAATGATCAAAAAGGCCAACTTTCCAATTCCTCATAGCTTATCAGACGAAATCGCGGAGCTTAAGGCGTCTAAGGGAGCCGAATCAAGGGCCGACTCTAAGGAAATACCTAAAGCGATTTCTCCGGTCAACTCCCAAGCTGCTTCCGAGGATACATCTGCCGAGGGCAAGGTAATCAAGATTGCATATCGCCTCATAGAAATATGTAAATCAGATTAA

Protein Analysis

674

Amino Acids

77.2

Weight (kDa)

9.21

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 157 - 332 1.2e-06 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1449
AccB1I GGYRCC 1 cut(s) 1449
AccB7I CCANNNNNTGG 1 cut(s) 78
AccI GTMKAC 1 cut(s) 334
AccII CGCG 1 cut(s) 1859
AciI CCGC 3 cut(s) 112, 678, 1859
AclWI GGATC 1 cut(s) 98
AcsI RAATTY 4 cut(s) 440, 1228, 1415, 1713
AcuI CTGAAG 2 cut(s) 1173, 1730
AcyI GRCGYC 1 cut(s) 1871
AfaI GTAC 5 cut(s) 269, 347, 1328, 1451, 1737
AfiI CCNNNNNNNGG 3 cut(s) 78, 1189, 1890
AflII CTTAAG 2 cut(s) 10, 1865
AjnI CCWGG 3 cut(s) 645, 743, 1717
AjuI GAANNNNNNNTTGG 2 cut(s) 1676, 1708
AluBI AGCT 9 cut(s) 9, 526, 1304, 1573, 1640, 1663, 1842, 1864, 1943
AluI AGCT 9 cut(s) 9, 526, 1304, 1573, 1640, 1663, 1842, 1864, 1943
Alw21I GWGCWC 2 cut(s) 697, 1063
Alw26I GTCTC 1 cut(s) 25
AlwI GGATC 1 cut(s) 98
AoxI GGCC 2 cut(s) 1818, 1893
ApeKI GCWGC 3 cut(s) 888, 1637, 1943
ApoI RAATTY 4 cut(s) 440, 1228, 1415, 1713
Asp718I GGTACC 1 cut(s) 1449
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 3 cut(s) 565, 649, 1893
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 2 cut(s) 16, 1811
AsuII TTCGAA 1 cut(s) 1082
AvaII GGWCC 2 cut(s) 565, 649
BanI GGYRCC 1 cut(s) 1449
BarI GAAGNNNNNNTAC 4 cut(s) 1442, 1474, 1728, 1760
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 2 cut(s) 697, 1063
BbvI GCAGC 3 cut(s) 875, 1649, 1930
BccI CCATC 4 cut(s) 975, 1037, 1204, 1379
BciT130I CCWGG 3 cut(s) 647, 745, 1719
BciVI GTATCC 2 cut(s) 979, 1948
BclI TGATCA 2 cut(s) 1438, 1809
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 25
BfaI CTAG 2 cut(s) 1428, 1770
BfrI CTTAAG 2 cut(s) 10, 1865
BfuI GTATCC 2 cut(s) 979, 1948
BglII AGATCT 1 cut(s) 1402
BisI GCNGC 3 cut(s) 889, 1638, 1944
BlsI GCNGC 3 cut(s) 890, 1639, 1945
Bme1390I CCNGG 4 cut(s) 43, 647, 745, 1719
Bme18I GGWCC 2 cut(s) 565, 649
BmgT120I GGNCC 3 cut(s) 565, 649, 1893
BmiI GGNNCC 3 cut(s) 567, 1451, 1882
BmrFI CCNGG 4 cut(s) 43, 647, 745, 1719
BmrI ACTGGG 3 cut(s) 66, 805, 1016
BmsI GCATC 4 cut(s) 221, 244, 1296, 1342
BmuI ACTGGG 3 cut(s) 66, 805, 1016
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 1 cut(s) 766
Bpu14I TTCGAA 1 cut(s) 1082
BpuEI CTTGAG 4 cut(s) 84, 645, 809, 1290
BpuMI CCSGG 1 cut(s) 43
BsaAI YACGTR 1 cut(s) 735
BsaHI GRCGYC 1 cut(s) 1871
BsaJI CCNNGG 6 cut(s) 42, 167, 369, 1793, 1950, 1965
BsaWI WCCGGW 2 cut(s) 389, 1927
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 1189, 1890
Bse1I ACTGG 6 cut(s) 39, 72, 800, 1022, 1174, 1488
Bse3DI GCAATG 2 cut(s) 422, 1616
BseBI CCWGG 3 cut(s) 647, 745, 1719
BseDI CCNNGG 6 cut(s) 42, 167, 369, 1793, 1950, 1965
BseGI GGATG 7 cut(s) 153, 358, 702, 781, 1063, 1174, 1390
BseLI CCNNNNNNNGG 3 cut(s) 78, 1189, 1890
BseMI GCAATG 2 cut(s) 422, 1616
BseMII CTCAG 1 cut(s) 1528
BseNI ACTGG 6 cut(s) 39, 72, 800, 1022, 1174, 1488
BseRI GAGGAG 5 cut(s) 87, 531, 648, 812, 879
BseXI GCAGC 3 cut(s) 875, 1649, 1930
BsgI GTGCAG 2 cut(s) 874, 1656
Bsh1236I CGCG 1 cut(s) 1859
BshFI GGCC 2 cut(s) 1820, 1895
BshNI GGYRCC 1 cut(s) 1449
BsiHKAI GWGCWC 2 cut(s) 697, 1063
BsiSI CCGG 4 cut(s) 42, 390, 968, 1928
BslFI GGGAC 3 cut(s) 95, 1004, 1162
BslI CCNNNNNNNGG 3 cut(s) 78, 1189, 1890
BsmAI GTCTC 1 cut(s) 25
BsmFI GGGAC 3 cut(s) 95, 1004, 1162
BsmI GAATGC 2 cut(s) 1597, 1732
BsnI GGCC 2 cut(s) 1820, 1895
Bsp119I TTCGAA 1 cut(s) 1082
Bsp1286I GDGCHC 2 cut(s) 697, 1063
Bsp1407I TGTACA 4 cut(s) 267, 345, 1326, 1735
Bsp143I GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
Bsp19I CCATGG 1 cut(s) 1793
BspACI CCGC 3 cut(s) 112, 678, 1859
BspANI GGCC 2 cut(s) 1820, 1895
BspCNI CTCAG 1 cut(s) 1527
BspFNI CGCG 1 cut(s) 1859
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 3 cut(s) 567, 1451, 1882
BspPI GGATC 1 cut(s) 98
BspT104I TTCGAA 1 cut(s) 1082
BspT107I GGYRCC 1 cut(s) 1449
BspTI CTTAAG 2 cut(s) 10, 1865
BsrDI GCAATG 2 cut(s) 422, 1616
BsrGI TGTACA 4 cut(s) 267, 345, 1326, 1735
BsrI ACTGG 6 cut(s) 39, 72, 800, 1022, 1174, 1488
BssECI CCNNGG 6 cut(s) 42, 167, 369, 1793, 1950, 1965
BssMI GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
BssNI GRCGYC 1 cut(s) 1871
BssT1I CCWWGG 1 cut(s) 1793
Bst2UI CCWGG 3 cut(s) 647, 745, 1719
Bst4CI ACNGT 6 cut(s) 324, 895, 943, 1314, 1326, 1634
Bst6I CTCTTC 2 cut(s) 1495, 1746
BstACI GRCGYC 1 cut(s) 1871
BstAFI CTTAAG 2 cut(s) 10, 1865
BstAPI GCANNNNNTGC 1 cut(s) 1601
BstAUI TGTACA 4 cut(s) 267, 345, 1326, 1735
BstBAI YACGTR 1 cut(s) 735
BstBI TTCGAA 1 cut(s) 1082
BstC8I GCNNGC 2 cut(s) 116, 594
BstDEI CTNAG 5 cut(s) 186, 1514, 1559, 1875, 1902
BstDSI CCRYGG 2 cut(s) 369, 1793
BstF5I GGATG 7 cut(s) 153, 358, 702, 781, 1063, 1174, 1390
BstFNI CGCG 1 cut(s) 1859
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 8 cut(s) 106, 366, 411, 783, 1321, 1405, 1441, 1812
BstMAI GTCTC 1 cut(s) 25
BstMBI GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
BstMWI GCNNNNNNNGC 1 cut(s) 1601
BstNI CCWGG 3 cut(s) 647, 745, 1719
BstNSI RCATGY 2 cut(s) 118, 596
BstSCI CCNGG 4 cut(s) 41, 645, 743, 1717
BstUI CGCG 1 cut(s) 1859
BstV1I GCAGC 3 cut(s) 875, 1649, 1930
BstV2I GAAGAC 1 cut(s) 911
BstX2I RGATCY 2 cut(s) 103, 1402
BstYI RGATCY 2 cut(s) 103, 1402
BsuI GTATCC 2 cut(s) 979, 1948
BsuRI GGCC 2 cut(s) 1820, 1895
BtgI CCRYGG 2 cut(s) 369, 1793
BtsCI GGATG 7 cut(s) 153, 358, 702, 781, 1063, 1174, 1390
BtsIMutI CAGTG 2 cut(s) 1481, 1639
Cac8I GCNNGC 2 cut(s) 116, 594
CciI TCATGA 1 cut(s) 1035
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 3 cut(s) 565, 649, 1893
CseI GACGC 1 cut(s) 1860
Csp6I GTAC 5 cut(s) 268, 346, 1327, 1450, 1736
CviQI GTAC 5 cut(s) 268, 346, 1327, 1450, 1736
DdeI CTNAG 5 cut(s) 186, 1514, 1559, 1875, 1902
DpnI GATC 8 cut(s) 105, 365, 410, 782, 1320, 1404, 1440, 1811
DpnII GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
Eam1104I CTCTTC 2 cut(s) 1495, 1746
EarI CTCTTC 2 cut(s) 1495, 1746
Eco130I CCWWGG 1 cut(s) 1793
Eco47I GGWCC 2 cut(s) 565, 649
Eco57I CTGAAG 2 cut(s) 1173, 1730
EcoO109I RGGNCCY 1 cut(s) 565
EcoRI GAATTC 1 cut(s) 1415
EcoRII CCWGG 3 cut(s) 645, 743, 1717
EcoT14I CCWWGG 1 cut(s) 1793
ErhI CCWWGG 1 cut(s) 1793
FalI AAGNNNNNCTT 6 cut(s) 40, 72, 1557, 1589, 1808, 1840
FaqI GGGAC 3 cut(s) 95, 1004, 1162
FauNDI CATATG 1 cut(s) 1477
FbaI TGATCA 2 cut(s) 1438, 1809
FblI GTMKAC 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 889, 1638, 1944
FokI GGATG 7 cut(s) 160, 365, 689, 788, 1050, 1181, 1397
Fsp4HI GCNGC 3 cut(s) 889, 1638, 1944
FspBI CTAG 2 cut(s) 1428, 1770
GlaI GCGC 1 cut(s) 20
GluI GCNGC 3 cut(s) 889, 1638, 1944
GsuI CTGGAG 1 cut(s) 766
HaeIII GGCC 2 cut(s) 1820, 1895
HapII CCGG 4 cut(s) 42, 390, 968, 1928
HgaI GACGC 1 cut(s) 1860
HhaI GCGC 1 cut(s) 21
Hin1I GRCGYC 1 cut(s) 1871
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 1 cut(s) 1933
HindII GTYRAC 1 cut(s) 1933
HpaII CCGG 4 cut(s) 42, 390, 968, 1928
HphI GGTGA 2 cut(s) 16, 1811
Hpy166II GTNNAC 4 cut(s) 335, 546, 1738, 1933
Hpy188I TCNGA 8 cut(s) 495, 507, 947, 1365, 1443, 1849, 1951, 2017
Hpy188III TCNNGA 8 cut(s) 101, 293, 826, 1036, 1420, 1462, 1748, 1981
Hpy8I GTNNAC 4 cut(s) 335, 546, 1738, 1933
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 5 cut(s) 129, 205, 580, 1277, 1468
HpyCH4III ACNGT 6 cut(s) 324, 895, 943, 1314, 1326, 1634
HpyCH4IV ACGT 2 cut(s) 289, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 1601
HpyF3I CTNAG 5 cut(s) 186, 1514, 1559, 1875, 1902
HpySE526I ACGT 2 cut(s) 289, 734
Hsp92I GRCGYC 1 cut(s) 1871
HspAI GCGC 1 cut(s) 19
KpnI GGTACC 1 cut(s) 1453
Ksp22I TGATCA 2 cut(s) 1438, 1809
Kzo9I GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
LmnI GCTCC 7 cut(s) 100, 433, 523, 1431, 1570, 1861, 1880
Lsp1109I GCAGC 3 cut(s) 875, 1649, 1930
LweI GCATC 4 cut(s) 221, 244, 1296, 1342
MaeI CTAG 2 cut(s) 1428, 1770
MaeII ACGT 2 cut(s) 289, 734
MalI GATC 8 cut(s) 105, 365, 410, 782, 1320, 1404, 1440, 1811
MboI GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
MfeI CAATTG 2 cut(s) 263, 972
MflI RGATCY 2 cut(s) 103, 1402
MhlI GDGCHC 2 cut(s) 697, 1063
MlyI GAGTC 3 cut(s) 340, 1481, 1892
MmeI TCCRAC 2 cut(s) 753, 1674
MseI TTAA 9 cut(s) 11, 401, 444, 588, 755, 936, 1394, 1866, 2020
MslI CAYNNNNRTG 2 cut(s) 927, 1379
MspCI CTTAAG 2 cut(s) 10, 1865
MspI CCGG 4 cut(s) 42, 390, 968, 1928
MspR9I CCNGG 4 cut(s) 43, 647, 745, 1719
MunI CAATTG 2 cut(s) 263, 972
Mva1269I GAATGC 2 cut(s) 1597, 1732
MvaI CCWGG 3 cut(s) 647, 745, 1719
MvnI CGCG 1 cut(s) 1859
MwoI GCNNNNNNNGC 1 cut(s) 1601
NciI CCSGG 1 cut(s) 43
NcoI CCATGG 1 cut(s) 1793
NdeI CATATG 1 cut(s) 1477
NdeII GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
NlaIV GGNNCC 3 cut(s) 567, 1451, 1882
NmeAIII GCCGAG 1 cut(s) 1990
NspI RCATGY 2 cut(s) 118, 596
NspV TTCGAA 1 cut(s) 1082
PaeI GCATGC 2 cut(s) 118, 596
PagI TCATGA 1 cut(s) 1035
PctI GAATGC 2 cut(s) 1597, 1732
PfeI GAWTC 7 cut(s) 964, 1076, 1520, 1555, 1759, 1775, 1886
PflMI CCANNNNNTGG 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 645
PkrI GCNGC 3 cut(s) 890, 1639, 1945
PleI GAGTC 3 cut(s) 339, 1481, 1892
PpsI GAGTC 3 cut(s) 339, 1481, 1892
Ppu21I YACGTR 1 cut(s) 735
PpuMI RGGWCCY 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 565
Psp6I CCWGG 3 cut(s) 645, 743, 1717
PspGI CCWGG 3 cut(s) 645, 743, 1717
PspN4I GGNNCC 3 cut(s) 567, 1451, 1882
PspPI GGNCC 3 cut(s) 565, 649, 1893
PspPPI RGGWCCY 1 cut(s) 565
PsuI RGATCY 2 cut(s) 103, 1402
RsaI GTAC 5 cut(s) 269, 347, 1328, 1451, 1737
RsaNI GTAC 5 cut(s) 268, 346, 1327, 1450, 1736
RseI CAYNNNNRTG 2 cut(s) 927, 1379
SaqAI TTAA 9 cut(s) 11, 401, 444, 588, 755, 936, 1394, 1866, 2020
SatI GCNGC 3 cut(s) 889, 1638, 1944
Sau3AI GATC 8 cut(s) 103, 363, 408, 780, 1318, 1402, 1438, 1809
Sau96I GGNCC 3 cut(s) 565, 649, 1893
SchI GAGTC 3 cut(s) 340, 1481, 1892
ScrFI CCNGG 4 cut(s) 43, 647, 745, 1719
SduI GDGCHC 2 cut(s) 697, 1063
SfaNI GCATC 4 cut(s) 221, 244, 1296, 1342
SfuI TTCGAA 1 cut(s) 1082
SinI GGWCC 2 cut(s) 565, 649
SmiMI CAYNNNNRTG 2 cut(s) 927, 1379
SmlI CTYRAG 6 cut(s) 10, 99, 660, 824, 1305, 1865
SmoI CTYRAG 6 cut(s) 10, 99, 660, 824, 1305, 1865
SphI GCATGC 2 cut(s) 118, 596
SsiI CCGC 3 cut(s) 112, 678, 1859
SspMI CTAG 2 cut(s) 1428, 1770
StyD4I CCNGG 4 cut(s) 41, 645, 743, 1717
StyI CCWWGG 1 cut(s) 1793
TaaI ACNGT 6 cut(s) 324, 895, 943, 1314, 1326, 1634
TaiI ACGT 2 cut(s) 292, 737
TaqI TCGA 3 cut(s) 1082, 1317, 1350
TatI WGTACW 4 cut(s) 267, 345, 1326, 1735
TfiI GAWTC 7 cut(s) 964, 1076, 1520, 1555, 1759, 1775, 1886
Tru1I TTAA 9 cut(s) 11, 401, 444, 588, 755, 936, 1394, 1866, 2020
Tru9I TTAA 9 cut(s) 11, 401, 444, 588, 755, 936, 1394, 1866, 2020
TscAI CASTG 2 cut(s) 1488, 1639
TseI GCWGC 3 cut(s) 888, 1637, 1943
TspGWI ACGGA 2 cut(s) 386, 551
TspRI CASTG 2 cut(s) 1488, 1639
Van91I CCANNNNNTGG 1 cut(s) 78
Vha464I CTTAAG 2 cut(s) 10, 1865
VpaK11BI GGWCC 2 cut(s) 565, 649
XapI RAATTY 4 cut(s) 440, 1228, 1415, 1713
XceI RCATGY 2 cut(s) 118, 596
XmiI GTMKAC 1 cut(s) 334
XspI CTAG 2 cut(s) 1428, 1770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.