pycom05g22980

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007335
Physical Location & Seq
Forward (+)
25068966 .. 25073853
4888 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4035 bp
ATGTTTGAACTAATCAAGAGTTTTAAGCACTGCCAATTATCCTTAAAGAAGAATCGTCCCACACGGTTTTCTATCTTTTTTCAATCAGAATTGTTCACAAGAATAAAGAAGAAGAAGAAGAAAAATGATGAGGGTCAAATCCAATATGGGATGCCTTTCCATAATTTTCTCCCTTTTCGACAACACCCCTTCCTTTTCATGATCATTGACTATATATGTTCTTCGTTTGCATTATCCTCAGAAAAATTCAAAGACCAAATTGTTTTCTTTCTCAAAAAGTTAATTTATAATTCAATTACAATATATATATATATATATATATATATATTAGCTTATCAAATATATAATAGATGGTGCAAGAGTTTTTTTTTTCTCCTTCAAAACGATGCTTCAAGTATTTTAATTATAAATGTCTATCAAAATATCAAAAGTTTATGGTTAAATGTATTTGCATTAATCATGCAAATTGTGGGAGCATTGCATATATTCAACTCTCATCAGCCCTTAAAAATAGGGCTGGATTGGATTCTTAAAGGAAAATGCTCAAAAGAAGGGAACTGTGTGTTGTTGGCTTGCTGCCATTTAATCATCACAAAGGTCTTCCACTTGAGTGCCAAATTCTCATCTCCTTCCCTCCTCTCTTCTCACATTTTATTTTTTTGTCTTATTGTCTCTATAAAAAAATCAATACAAGATAGAAGGAGAGAGAATCCTCAATTTGATTTATTAGTTCAAATAGGAGGGGAAGGAAGGGAAGAGAGATTAAGAGGGGATAATCCTCCATGCTTTTCAGATACCCTTGCGAGAGGCACTCAATTTGGTTTATTAGTGCATAAATATGATAATTCCCCTGTGAATTGTGCCAATCCCATATACTGGAGGTTTTTCATGTTTGGAAGTACTTTTAAAGTTCTAAAAATGCTTGTAGCATTTTATACGTTGAAAGCACTTCTTGCAACATTGGTAGAAAAACTTAAAAATACTTTTTGTGATAATCACATGTTACGTGTTTTTCTGGAATGCAATAGTCATATACAAACCTTAGAAGTATTTCATAAACGTAATTGGATTATAATTTTTTTAGTGCAATCCTACACAAACATCAAACATATTTACCATATAAAAGGGAAGAATAACAACAACCACGCCTTATCTCATCAAGTGAGATCGGCTGTTAGGAATCATATCATAGGATTTAACGTGAAGAACAAAGTGTCGGTTTTCGACTACCTCACACACTCCCCCTTCTCCTTTATCCGGTTACCATATAAATGGGAGCATAATGAGAAATTAAAAGCTAGTGCACGACAGTCTGAGATAATTTTACACATGCGGAATTTACCATATAAAAGGGAAAACTTAGACAGGAGAAGTTTACCATACAAAAGGAAGCATAATCATATAAGTACCCCCAAGAGAATAATTACAATGGATTATGGTGAATGGATCTTGGAAGATATTAATTTTCTTGTAAATGAATGTGATTTAGTTAATGTTGTCATTACATTTGCTATAAAACTAGTCATTGCTAATGAAAGGTATATATTCAGTATTAAAACGAAACTAACATTTTTGTATTGTGAGCTCAAATCCGGTCCCATTTTTTTCTCACATATGAATTTAGGGTACAATGAATCAAAATTAACAAGAGTGTATGAAAGACTTTCACATTGTATAGTGAATAATACACAATTGTACATCAACAGATTCAAATCTATAAAATTACAAAAATTAATTGCGGCCATAAAAGTTCATCAAAGAAAAGCCCAATATTCAAATTCCTACAGGCCCGGCCCGTCCAAATTAATGAAAATAATAATTCAAGGGAAAAAAAAAAAACTTGAAAGCTACGAAGATTGGTGGGCAGTGGAACCAAACGAACTGAGAAACTTCATGGTTTCTCTGCGAATTCTGAGAACCCATGAACTCCAACTGAAGCTATTATCCCCTGCTTCAACCCCCATTTCTCTATTCTACACCCTCTTCTCTCTCAGAACACTCTCTTCCTACACTCACTATGATGACCAAAACTCCACCACCACTTCCACCGCCACCGCCGCCGCCGCCGCCGCTTCTTCTCAATCGCAGTCACTTGTCAGCTCGATCTGCTCATTGGTATACCAATCCTATTCCCCGCAAACCCATCTGAGATCTTCGCCTCCGAAGCTCAATCTCGACTTAAATCCTGAATCTTTGACCCACGAGCAGGCCATTTCCGTCGTTGCTTCACTGGCTCAGGAAGCAGGTTCCATGGCGGCGCTGAGTTTCTTTAACTGGGCAATTGGGTTTCCCAAGTTTCGATATTTCATGCGGCTTTACATATTCTGCGCAATGGCGATACTTCGCAATGGGAATCTGGAGAGAGCCCATGAGGTGGTTCATTGTATGGTGATGAATTTCGCTGAGATTGAGAGGTTGAAGGAGGCTGCTGATATGGTCTTTGAGATACAGAATCAGGGTCTTGCGTTGAGTACTCGGACGTTGAATTGTGTTCTTGGGATTGCTTGCGATTTGGGTTTGGTTGAGTATGCAGAGAATCTGTTCGAGGAAATGTGCGACAGAGGTGTGTCTCCTGATTCCTTGAGCTATAAGTCTATGGTTGTTGGGTTTTGTAGGAACGGGAGGGTTTTGGACGTGGATAGGTGGTTGAGTAAGATGCTCGAGAGAGGTTTTGTTTTGGATAATGCATCGTTTACTTTGATCATCAACATGTTTTGCGAGAAGGGTCTTGTAGGTCGAGCGTCTTGGTGTTTTGATAAGATGATTAGAATGGGTGTGAAGCCGAATTTGATTAATTTCACGACGATGATTAATGGGTTGTGCAAGAGGGGCAGCATTAAACAAGCATTTGAAATGTTGGAGGAAATGGTGAGGAAAGGTTGGAAGCCGAATGTGTATACACACACAGTATTGATTCATGGGCTCTGCAAGAAAGGCTGGACTGAAAGGGCGTTTAGACTGTTTCTTAAGCTTGTCCGGAGCGATAATCACAAGCCAAATGTACATACATATACTGCTATGATCAATGGATATTGCCAAGAGGACAAAATGAGCCGCGCAGAGATGTTATTGAGCAGGATGAAGGAACAAGGATTAGTTCCTAACACCAACACGTATACCACTCTAGTTTCAGGGCATTGTAAAGCTGGGAACTTTGAAAGAGCATATGAATTTATGGATATAATGGATAAGGAAGGATTCCCTCCTAATATCTATACATACAATGCAGTAATTGACAGTCTCTGTAAAAGGGGAAGGGTTGAAGAAGCAAATAAACTGATTAGGAAGGCCTTTCGCCGAGGATTGGCAGCTGATAGGGTCACGTATACAATTTTCATATCTGAGCACTGCAAACGGGCTGATATTAATGGGGCCCTGGTGTTTTTTACCAAGATGGTGAAGGTTGGATTGCAGCCTGATATACATTTGTATACAACTTTGATTGCTGCCTACTGCAGGCAAAAGAAAATGAAAGAAAGCGAGAAACTTCTTGAGTTTGCTGTCAGGGCTGACTTGATTCCCACCAAGGAAACCTATACATCCATGATTTGTGGGCATTGTCGGGATGGAAATATTGCTTCTGCTATTAAGTATTTCCAAAGGATGGGTGACCATAGTTGTGCGCCTGATAGTATTACTTACGGTGCTCTGATAAGTGGGCTCTGTAAGGACGAGAAATTGGAAGAGGCTCGTAGGTTATATGATACCATGATGGACAAAGGGCTATCGCCCTGTGAAGTCTCTAGATTTACATTAGCTTACAAGTACTGCATGAAAGATGACTCTGCCGCTGCTATGGTTATGTTAGAAAGGCTAGAAAAGAAGCTCTGGATCCGCACGGTGTATACATTAGTCAGGAAGCTTTGTAGTGAGAAGAAAGTAGGGATAGCAGCATTGTTCTTTCATAAATTAGTAGACAAGGACCAAAACGTGGACCGTGTGACATTGGCAGCGTTTATGACAGCATGCTATGAGAGCAACAAGTATGCTCTCGTTTCAGATTTGACTGAGAGGATCTCAAAAGGAATTGGCTAG

Protein Analysis

1345

Amino Acids

155.37

Weight (kDa)

9.43

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 825 - 872 3.3e-09 PPR repeat family
PPR_2 PF13041 896 - 942 1.8e-09 PPR repeat family
PPR_long PF17177 912 - 1060 2e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 924 - 957 9e-12 PPR repeat
PPR_2 PF13041 928 - 977 3.2e-18 PPR repeat family
PPR PF01535 933 - 960 8e-07 PPR repeat
PPR_3 PF13812 952 - 1011 2.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 959 - 988 8.1e-09 PPR repeat
PPR_2 PF13041 969 - 1013 1.7e-10 PPR repeat family
TPR_24 PF23276 991 - 1090 1.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 993 - 1039 1.7e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 996 - 1028 3.4e-09 PPR repeat
PPR_2 PF13041 999 - 1048 1.6e-16 PPR repeat family
PPR PF01535 1003 - 1032 1.2e-07 PPR repeat
PPR_3 PF13812 1023 - 1080 2.6e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 1031 - 1062 2.9e-10 PPR repeat
PPR_2 PF13041 1034 - 1083 1.5e-15 PPR repeat family
PPR PF01535 1038 - 1067 9e-06 PPR repeat
PPR_1 PF12854 1066 - 1096 1.3e-09 PPR repeat
PPR_2 PF13041 1071 - 1118 6.8e-12 PPR repeat family
PPR_2 PF13041 1105 - 1153 3.1e-10 PPR repeat family
PPR_2 PF13041 1139 - 1188 1.3e-09 PPR repeat family
PPR_1 PF12854 1172 - 1202 3.3e-06 PPR repeat
PPR_2 PF13041 1177 - 1223 8.2e-13 PPR repeat family
PPR_1 PF12854 1206 - 1237 3.3e-09 PPR repeat
PPR_2 PF13041 1209 - 1244 4.9e-08 PPR repeat family
PPR PF01535 1212 - 1242 3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 288, 407, 1073
Acc16I TGCGCA 1 cut(s) 2329
Acc36I ACCTGC 1 cut(s) 2234
AccB7I CCANNNNNTGG 4 cut(s) 876, 2374, 3604, 3931
AccI GTMKAC 7 cut(s) 2118, 2897, 3116, 3326, 3431, 3845, 3915
AccII CGCG 1 cut(s) 3057
AccIII TCCGGA 1 cut(s) 2976
AclWI GGATC 4 cut(s) 1454, 3826, 3839, 4022
AcoI YGGCCR 1 cut(s) 1740
AcsI RAATTY 9 cut(s) 245, 617, 1336, 1618, 1777, 1908, 2395, 2785, 3170
AcuI CTGAAG 1 cut(s) 1955
AfaI GTAC 7 cut(s) 901, 1408, 1628, 1697, 2473, 3003, 3767
AfiI CCNNNNNNNGG 8 cut(s) 876, 1257, 2206, 2374, 3304, 3456, 3604, 3931
AflII CTTAAG 1 cut(s) 2966
AflIII ACRYGT 4 cut(s) 999, 1006, 2709, 3111
AhlI ACTAGT 1 cut(s) 1519
AjiI CACGTC 1 cut(s) 2635
AjnI CCWGG 1 cut(s) 3375
AleI CACNNNNGTG 1 cut(s) 609
Alw21I GWGCWC 4 cut(s) 1306, 1587, 3348, 3649
Alw26I GTCTC 4 cut(s) 676, 2574, 3245, 3745
Alw44I GTGCAC 1 cut(s) 1302
AlwI GGATC 4 cut(s) 1454, 3826, 3839, 4022
AlwNI CAGNNNCTG 1 cut(s) 3243
Ama87I CYCGRG 1 cut(s) 2660
Aor13HI TCCGGA 1 cut(s) 2976
AoxI GGCC 6 cut(s) 1740, 1787, 1792, 2208, 3288, 3372
ApaI GGGCCC 1 cut(s) 3376
ApaLI GTGCAC 1 cut(s) 1302
ApeKI GCWGC 9 cut(s) 576, 2426, 2832, 3308, 3412, 3446, 3791, 3890, 3950
ApoI RAATTY 9 cut(s) 245, 617, 1336, 1618, 1777, 1908, 2395, 2785, 3170
AseI ATTAAT 7 cut(s) 455, 1461, 1733, 1805, 2793, 2811, 3366
Asp700I GAANNNNTTC 2 cut(s) 1050, 2540
AspLEI GCGC 4 cut(s) 2260, 2330, 3059, 3625
AspS9I GGNCC 7 cut(s) 1595, 1788, 1793, 3372, 3373, 3922, 3934
AsuC2I CCSGG 1 cut(s) 1791
AsuHPI GGTGA 5 cut(s) 1451, 2401, 2881, 3409, 3620
AvaI CYCGRG 1 cut(s) 2660
AvaII GGWCC 3 cut(s) 1595, 3922, 3934
BaeGI GKGCMC 2 cut(s) 1306, 3376
BamHI GGATCC 1 cut(s) 3831
BanII GRGCYC 5 cut(s) 1587, 2368, 2925, 3376, 3663
BauI CACGAG 1 cut(s) 2201
BbsI GAAGAC 1 cut(s) 592
Bbv12I GWGCWC 4 cut(s) 1306, 1587, 3348, 3649
BbvI GCAGC 9 cut(s) 563, 2413, 2844, 3320, 3424, 3433, 3778, 3902, 3962
BccI CCATC 6 cut(s) 345, 2151, 3388, 3560, 3598, 3706
BciT130I CCWGG 1 cut(s) 3377
BclI TGATCA 3 cut(s) 201, 2700, 3021
BcnI CCSGG 1 cut(s) 1791
BcoDI GTCTC 4 cut(s) 676, 2574, 3245, 3745
BcuI ACTAGT 1 cut(s) 1519
BfaI CTAG 6 cut(s) 1299, 1520, 3125, 3744, 3815, 4033
BfmI CTRYAG 2 cut(s) 1783, 3454
BfoI RGCGCY 1 cut(s) 2261
BfrI CTTAAG 1 cut(s) 2966
BfuAI ACCTGC 1 cut(s) 2234
BglII AGATCT 1 cut(s) 2150
BmcAI AGTACT 3 cut(s) 901, 2473, 3767
Bme1390I CCNGG 2 cut(s) 1791, 3377
Bme18I GGWCC 3 cut(s) 1595, 3922, 3934
BmeT110I CYCGRG 1 cut(s) 2660
BmgBI CACGTC 1 cut(s) 2635
BmgT120I GGNCC 7 cut(s) 1595, 1788, 1793, 3372, 3373, 3922, 3934
BmiI GGNNCC 6 cut(s) 1597, 1872, 2248, 3373, 3374, 3833
BmrFI CCNGG 2 cut(s) 1791, 3377
BmrI ACTGGG 1 cut(s) 2284
BmsI GCATC 4 cut(s) 141, 376, 2646, 2696
BmuI ACTGGG 1 cut(s) 2284
BpiI GAAGAC 1 cut(s) 592
BplI GAGNNNNNCTC 4 cut(s) 796, 828, 4001, 4033
BpmI CTGGAG 2 cut(s) 898, 2378
Bpu10I CCTNAGC 1 cut(s) 2235
BpuEI CTTGAG 3 cut(s) 628, 2602, 3512
BpuMI CCSGG 1 cut(s) 1791
BsaAI YACGTR 3 cut(s) 1007, 3114, 3324
BsaBI GATNNNNATC 1 cut(s) 1711
BsaJI CCNNGG 4 cut(s) 2250, 3298, 3375, 3525
BsaWI WCCGGW 3 cut(s) 1257, 1592, 2976
BsaXI ACNNNNNCTCC 2 cut(s) 2015, 2045
Bsc4I CCNNNNNNNGG 8 cut(s) 876, 1257, 2206, 2374, 3304, 3456, 3604, 3931
Bse1I ACTGG 3 cut(s) 881, 2235, 2279
Bse3DI GCAATG 4 cut(s) 476, 1524, 2337, 2353
Bse8I GATNNNNATC 1 cut(s) 1711
BseAI TCCGGA 1 cut(s) 2976
BseBI CCWGG 1 cut(s) 3377
BseDI CCNNGG 4 cut(s) 2250, 3298, 3375, 3525
BseGI GGATG 5 cut(s) 156, 3084, 3539, 3571, 3609
BseJI GATNNNNATC 1 cut(s) 1711
BseLI CCNNNNNNNGG 8 cut(s) 876, 1257, 2206, 2374, 3304, 3456, 3604, 3931
BseMI GCAATG 4 cut(s) 476, 1524, 2337, 2353
BseNI ACTGG 3 cut(s) 881, 2235, 2279
BseRI GAGGAG 1 cut(s) 626
BseSI GKGCMC 2 cut(s) 1306, 3376
BseXI GCAGC 9 cut(s) 563, 2413, 2844, 3320, 3424, 3433, 3778, 3902, 3962
BseYI CCCAGC 1 cut(s) 3146
Bsh1236I CGCG 1 cut(s) 3057
BshFI GGCC 6 cut(s) 1742, 1789, 1794, 2210, 3290, 3374
BsiHKAI GWGCWC 4 cut(s) 1306, 1587, 3348, 3649
BsiHKCI CYCGRG 1 cut(s) 2660
BsiSI CCGG 4 cut(s) 1258, 1593, 1791, 2977
BslFI GGGAC 2 cut(s) 42, 1581
BslI CCNNNNNNNGG 8 cut(s) 876, 1257, 2206, 2374, 3304, 3456, 3604, 3931
BsmAI GTCTC 4 cut(s) 676, 2574, 3245, 3745
BsmFI GGGAC 2 cut(s) 42, 1581
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 6 cut(s) 1742, 1789, 1794, 2210, 3290, 3374
BsoBI CYCGRG 1 cut(s) 2660
Bsp120I GGGCCC 1 cut(s) 3372
Bsp1286I GDGCHC 8 cut(s) 1306, 1587, 2368, 2925, 3348, 3376, 3649, 3663
Bsp13I TCCGGA 1 cut(s) 2976
Bsp1407I TGTACA 2 cut(s) 1695, 3001
Bsp143I GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
Bsp19I CCATGG 1 cut(s) 2250
BspANI GGCC 6 cut(s) 1742, 1789, 1794, 2210, 3290, 3374
BspEI TCCGGA 1 cut(s) 2976
BspFNI CGCG 1 cut(s) 3057
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 6 cut(s) 1597, 1872, 2248, 3373, 3374, 3833
BspMAI CTGCAG 1 cut(s) 3458
BspMI ACCTGC 1 cut(s) 2234
BspPI GGATC 4 cut(s) 1454, 3826, 3839, 4022
BspTI CTTAAG 1 cut(s) 2966
BsrDI GCAATG 4 cut(s) 476, 1524, 2337, 2353
BsrGI TGTACA 2 cut(s) 1695, 3001
BsrI ACTGG 3 cut(s) 881, 2235, 2279
BssECI CCNNGG 4 cut(s) 2250, 3298, 3375, 3525
BssMI GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
BssNAI GTATAC 6 cut(s) 2119, 2898, 3117, 3327, 3432, 3846
BssSI CACGAG 1 cut(s) 2201
BssT1I CCWWGG 2 cut(s) 2250, 3525
Bst1107I GTATAC 6 cut(s) 2119, 2898, 3117, 3327, 3432, 3846
Bst2BI CACGAG 1 cut(s) 2201
Bst2UI CCWGG 1 cut(s) 3377
Bst4CI ACNGT 9 cut(s) 66, 560, 1311, 2908, 2961, 3239, 3644, 3841, 3938
Bst6I CTCTTC 5 cut(s) 646, 750, 1988, 2008, 3678
BstAFI CTTAAG 1 cut(s) 2966
BstAPI GCANNNNNTGC 1 cut(s) 953
BstAUI TGTACA 2 cut(s) 1695, 3001
BstBAI YACGTR 3 cut(s) 1007, 3114, 3324
BstC8I GCNNGC 5 cut(s) 574, 2208, 2506, 3458, 3967
BstDSI CCRYGG 1 cut(s) 2250
BstEII GGTNACC 2 cut(s) 1260, 3608
BstENI CCTNNNNNAGG 1 cut(s) 3454
BstF5I GGATG 5 cut(s) 156, 3084, 3539, 3571, 3609
BstFNI CGCG 1 cut(s) 3057
BstH2I RGCGCY 1 cut(s) 2261
BstHHI GCGC 4 cut(s) 2260, 2330, 3059, 3625
BstKTI GATC 9 cut(s) 204, 1169, 1449, 2106, 2153, 2703, 3024, 3834, 4017
BstMAI GTCTC 4 cut(s) 676, 2574, 3245, 3745
BstMBI GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
BstNI CCWGG 1 cut(s) 3377
BstNSI RCATGY 4 cut(s) 1003, 1333, 2713, 3969
BstPI GGTNACC 2 cut(s) 1260, 3608
BstSCI CCNGG 2 cut(s) 1789, 3375
BstSFI CTRYAG 2 cut(s) 1783, 3454
BstSLI GKGCMC 2 cut(s) 1306, 3376
BstUI CGCG 1 cut(s) 3057
BstV1I GCAGC 9 cut(s) 563, 2413, 2844, 3320, 3424, 3433, 3778, 3902, 3962
BstV2I GAAGAC 1 cut(s) 592
BstX2I RGATCY 4 cut(s) 1446, 2150, 3831, 4014
BstXI CCANNNNNNTGG 1 cut(s) 1272
BstYI RGATCY 4 cut(s) 1446, 2150, 3831, 4014
BstZ17I GTATAC 6 cut(s) 2119, 2898, 3117, 3327, 3432, 3846
BsuRI GGCC 6 cut(s) 1742, 1789, 1794, 2210, 3290, 3374
BtgI CCRYGG 1 cut(s) 2250
BtrI CACGTC 1 cut(s) 2635
BtsCI GGATG 5 cut(s) 156, 3084, 3539, 3571, 3609
BtsI GCAGTG 3 cut(s) 28, 1872, 3346
BtsIMutI CAGTG 4 cut(s) 28, 1872, 2228, 3346
BveI ACCTGC 1 cut(s) 2234
Cac8I GCNNGC 5 cut(s) 574, 2208, 2506, 3458, 3967
CaiI CAGNNNCTG 1 cut(s) 3243
CciI TCATGA 1 cut(s) 198
CfoI GCGC 4 cut(s) 2260, 2330, 3059, 3625
Cfr13I GGNCC 7 cut(s) 1595, 1788, 1793, 3372, 3373, 3922, 3934
CseI GACGC 1 cut(s) 2730
Csp6I GTAC 7 cut(s) 900, 1407, 1627, 1696, 2472, 3002, 3766
CviQI GTAC 7 cut(s) 900, 1407, 1627, 1696, 2472, 3002, 3766
DpnI GATC 9 cut(s) 203, 1168, 1448, 2105, 2152, 2702, 3023, 3833, 4016
DpnII GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
DraI TTTAAA 1 cut(s) 907
EaeI YGGCCR 1 cut(s) 1740
Eam1104I CTCTTC 5 cut(s) 646, 750, 1988, 2008, 3678
EarI CTCTTC 5 cut(s) 646, 750, 1988, 2008, 3678
Ecl136II GAGCTC 1 cut(s) 1585
Eco130I CCWWGG 2 cut(s) 2250, 3525
Eco147I AGGCCT 1 cut(s) 3290
Eco24I GRGCYC 5 cut(s) 1587, 2368, 2925, 3376, 3663
Eco47I GGWCC 3 cut(s) 1595, 3922, 3934
Eco53kI GAGCTC 1 cut(s) 1585
Eco57I CTGAAG 1 cut(s) 1955
Eco88I CYCGRG 1 cut(s) 2660
Eco91I GGTNACC 2 cut(s) 1260, 3608
EcoICRI GAGCTC 1 cut(s) 1585
EcoNI CCTNNNNNAGG 1 cut(s) 3454
EcoO109I RGGNCCY 2 cut(s) 3372, 3373
EcoO65I GGTNACC 2 cut(s) 1260, 3608
EcoRI GAATTC 1 cut(s) 1908
EcoRII CCWGG 1 cut(s) 3375
EcoT14I CCWWGG 2 cut(s) 2250, 3525
EcoT22I ATGCAT 1 cut(s) 2689
EcoT38I GRGCYC 5 cut(s) 1587, 2368, 2925, 3376, 3663
ErhI CCWWGG 2 cut(s) 2250, 3525
FalI AAGNNNNNCTT 2 cut(s) 936, 968
FaqI GGGAC 2 cut(s) 42, 1581
FauI CCCGC 1 cut(s) 2142
FauNDI CATATG 2 cut(s) 1614, 3166
FbaI TGATCA 3 cut(s) 201, 2700, 3021
FblI GTMKAC 7 cut(s) 2118, 2897, 3116, 3326, 3431, 3845, 3915
FokI GGATG 5 cut(s) 163, 3091, 3526, 3578, 3616
FriOI GRGCYC 5 cut(s) 1587, 2368, 2925, 3376, 3663
FspBI CTAG 6 cut(s) 1299, 1520, 3125, 3744, 3815, 4033
FspI TGCGCA 1 cut(s) 2329
GlaI GCGC 4 cut(s) 2259, 2329, 3058, 3624
GsaI CCCAGC 1 cut(s) 3150
GsuI CTGGAG 2 cut(s) 898, 2378
HaeII RGCGCY 1 cut(s) 2261
HaeIII GGCC 6 cut(s) 1742, 1789, 1794, 2210, 3290, 3374
HapII CCGG 4 cut(s) 1258, 1593, 1791, 2977
HgaI GACGC 1 cut(s) 2730
HhaI GCGC 4 cut(s) 2260, 2330, 3059, 3625
Hin6I GCGC 4 cut(s) 2258, 2328, 3057, 3623
HinP1I GCGC 4 cut(s) 2258, 2328, 3057, 3623
HindIII AAGCTT 2 cut(s) 2969, 3860
HpaII CCGG 4 cut(s) 1258, 1593, 1791, 2977
HphI GGTGA 5 cut(s) 1451, 2401, 2881, 3409, 3620
Hpy99I CGWCG 2 cut(s) 2222, 2806
HpyCH4III ACNGT 9 cut(s) 66, 560, 1311, 2908, 2961, 3239, 3644, 3841, 3938
HpyCH4IV ACGT 9 cut(s) 938, 1006, 1060, 1200, 2480, 2634, 3113, 3323, 3930
HpySE526I ACGT 9 cut(s) 938, 1006, 1060, 1200, 2480, 2634, 3113, 3323, 3930
HspAI GCGC 4 cut(s) 2258, 2328, 3057, 3623
Kpn2I TCCGGA 1 cut(s) 2976
Ksp22I TGATCA 3 cut(s) 201, 2700, 3021
Kzo9I GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
LmnI GCTCC 3 cut(s) 473, 1276, 2979
Lsp1109I GCAGC 9 cut(s) 563, 2413, 2844, 3320, 3424, 3433, 3778, 3902, 3962
LweI GCATC 4 cut(s) 141, 376, 2646, 2696
MaeI CTAG 6 cut(s) 1299, 1520, 3125, 3744, 3815, 4033
MaeII ACGT 9 cut(s) 938, 1006, 1060, 1200, 2480, 2634, 3113, 3323, 3930
MaeIII GTNAC 6 cut(s) 1002, 1260, 2088, 3319, 3608, 3940
MalI GATC 9 cut(s) 203, 1168, 1448, 2105, 2152, 2702, 3023, 3833, 4016
MboI GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
MfeI CAATTG 2 cut(s) 1691, 2280
MflI RGATCY 4 cut(s) 1446, 2150, 3831, 4014
MhlI GDGCHC 8 cut(s) 1306, 1587, 2368, 2925, 3348, 3376, 3649, 3663
MlyI GAGTC 1 cut(s) 3776
MmeI TCCRAC 4 cut(s) 1954, 2838, 2861, 3385
Mph1103I ATGCAT 1 cut(s) 2689
MroI TCCGGA 1 cut(s) 2976
MroXI GAANNNNTTC 2 cut(s) 1050, 2540
MslI CAYNNNNRTG 7 cut(s) 609, 837, 1270, 2019, 2708, 2804, 3618
MspA1I CMGCKG 2 cut(s) 3311, 3791
MspCI CTTAAG 1 cut(s) 2966
MspI CCGG 4 cut(s) 1258, 1593, 1791, 2977
MspR9I CCNGG 2 cut(s) 1791, 3377
MunI CAATTG 2 cut(s) 1691, 2280
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 1 cut(s) 3377
MvnI CGCG 1 cut(s) 3057
NciI CCSGG 1 cut(s) 1791
NcoI CCATGG 1 cut(s) 2250
NdeI CATATG 2 cut(s) 1614, 3166
NdeII GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
NlaIV GGNNCC 6 cut(s) 1597, 1872, 2248, 3373, 3374, 3833
NmeAIII GCCGAG 1 cut(s) 3323
NmuCI GTSAC 4 cut(s) 2088, 3319, 3608, 3940
NsbI TGCGCA 1 cut(s) 2329
NsiI ATGCAT 1 cut(s) 2689
NspI RCATGY 4 cut(s) 1003, 1333, 2713, 3969
OliI CACNNNNGTG 1 cut(s) 609
PaeI GCATGC 1 cut(s) 3969
PaeR7I CTCGAG 1 cut(s) 2660
PagI TCATGA 1 cut(s) 198
PceI AGGCCT 1 cut(s) 3290
PciI ACATGT 2 cut(s) 999, 2709
PctI GAATGC 1 cut(s) 1025
PdmI GAANNNNTTC 2 cut(s) 1050, 2540
PflMI CCANNNNNTGG 4 cut(s) 876, 2374, 3604, 3931
PleI GAGTC 1 cut(s) 3776
PpsI GAGTC 1 cut(s) 3776
Ppu21I YACGTR 3 cut(s) 1007, 3114, 3324
PscI ACATGT 2 cut(s) 999, 2709
PshBI ATTAAT 7 cut(s) 455, 1461, 1733, 1805, 2793, 2811, 3366
PsiI TTATAA 3 cut(s) 288, 407, 1073
Psp124BI GAGCTC 1 cut(s) 1587
Psp6I CCWGG 1 cut(s) 3375
PspEI GGTNACC 2 cut(s) 1260, 3608
PspFI CCCAGC 1 cut(s) 3146
PspGI CCWGG 1 cut(s) 3375
PspN4I GGNNCC 6 cut(s) 1597, 1872, 2248, 3373, 3374, 3833
PspOMI GGGCCC 1 cut(s) 3372
PspPI GGNCC 7 cut(s) 1595, 1788, 1793, 3372, 3373, 3922, 3934
PstI CTGCAG 1 cut(s) 3458
PstNI CAGNNNCTG 1 cut(s) 3243
PsuI RGATCY 4 cut(s) 1446, 2150, 3831, 4014
PvuII CAGCTG 1 cut(s) 3311
RsaI GTAC 7 cut(s) 901, 1408, 1628, 1697, 2473, 3003, 3767
RsaNI GTAC 7 cut(s) 900, 1407, 1627, 1696, 2472, 3002, 3766
RseI CAYNNNNRTG 7 cut(s) 609, 837, 1270, 2019, 2708, 2804, 3618
SacI GAGCTC 1 cut(s) 1587
Sau3AI GATC 9 cut(s) 201, 1166, 1446, 2103, 2150, 2700, 3021, 3831, 4014
Sau96I GGNCC 7 cut(s) 1595, 1788, 1793, 3372, 3373, 3922, 3934
ScaI AGTACT 3 cut(s) 901, 2473, 3767
SchI GAGTC 1 cut(s) 3776
ScrFI CCNGG 2 cut(s) 1791, 3377
SduI GDGCHC 8 cut(s) 1306, 1587, 2368, 2925, 3348, 3376, 3649, 3663
SfaNI GCATC 4 cut(s) 141, 376, 2646, 2696
SfcI CTRYAG 2 cut(s) 1783, 3454
Sfr274I CTCGAG 1 cut(s) 2660
SinI GGWCC 3 cut(s) 1595, 3922, 3934
SlaI CTCGAG 1 cut(s) 2660
SmiMI CAYNNNNRTG 7 cut(s) 609, 837, 1270, 2019, 2708, 2804, 3618
SmlI CTYRAG 5 cut(s) 607, 2581, 2660, 2966, 3491
SmoI CTYRAG 5 cut(s) 607, 2581, 2660, 2966, 3491
SpeI ACTAGT 1 cut(s) 1519
SphI GCATGC 1 cut(s) 3969
SseBI AGGCCT 1 cut(s) 3290
SspI AATATT 2 cut(s) 1772, 3574
SspMI CTAG 6 cut(s) 1299, 1520, 3125, 3744, 3815, 4033
SstI GAGCTC 1 cut(s) 1587
StuI AGGCCT 1 cut(s) 3290
StyD4I CCNGG 2 cut(s) 1789, 3375
StyI CCWWGG 2 cut(s) 2250, 3525
TaaI ACNGT 9 cut(s) 66, 560, 1311, 2908, 2961, 3239, 3644, 3841, 3938
TaiI ACGT 9 cut(s) 941, 1009, 1063, 1203, 2483, 2637, 3116, 3326, 3933
TaqI TCGA 8 cut(s) 178, 1224, 2102, 2175, 2299, 2544, 2661, 2737
TatI WGTACW 5 cut(s) 899, 1695, 2471, 3001, 3765
TscAI CASTG 4 cut(s) 35, 1872, 2235, 3353
TseFI GTSAC 4 cut(s) 2088, 3319, 3608, 3940
TseI GCWGC 9 cut(s) 576, 2426, 2832, 3308, 3412, 3446, 3791, 3890, 3950
Tsp45I GTSAC 4 cut(s) 2088, 3319, 3608, 3940
TspGWI ACGGA 1 cut(s) 2206
TspRI CASTG 4 cut(s) 35, 1872, 2235, 3353
Van91I CCANNNNNTGG 4 cut(s) 876, 2374, 3604, 3931
Vha464I CTTAAG 1 cut(s) 2966
VneI GTGCAC 1 cut(s) 1302
VpaK11BI GGWCC 3 cut(s) 1595, 3922, 3934
VspI ATTAAT 7 cut(s) 455, 1461, 1733, 1805, 2793, 2811, 3366
XagI CCTNNNNNAGG 1 cut(s) 3454
XapI RAATTY 9 cut(s) 245, 617, 1336, 1618, 1777, 1908, 2395, 2785, 3170
XbaI TCTAGA 1 cut(s) 3743
XceI RCATGY 4 cut(s) 1003, 1333, 2713, 3969
XhoI CTCGAG 1 cut(s) 2660
XmiI GTMKAC 7 cut(s) 2118, 2897, 3116, 3326, 3431, 3845, 3915
XmnI GAANNNNTTC 2 cut(s) 1050, 2540
XspI CTAG 6 cut(s) 1299, 1520, 3125, 3744, 3815, 4033
ZrmI AGTACT 3 cut(s) 901, 2473, 3767
Zsp2I ATGCAT 1 cut(s) 2689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.