pycom05g28790

Auxin-repressed 12.5 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0005809
Physical Location & Seq
Forward (+)
29385655 .. 29386402
748 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 351 bp
ATGGTTCTGCTTGAAAAGCTTTGGGATGATATTGTCGCCGGACCTCAGCCTGAACGTGGCCTTGGCATGCTTAGGAAGCCGTCTCCTAAGCCCTTGAATATCAAAGTGGAGGGAGAGTCGAGCAAGTTGGCGATGCCGATGAGCCCCGGAACACCAGGCACCCCAGGAACCCCGGGCACACCAGCTTCCGCTCGTGCTAAGGACAACGTTTGGAGGAGTGTGTTCCATCCAGGTAGCAACCTTGCCACCAAATCCATGGGCAACCAGGTTTTCGACAAGCCACAGCCCAACTCTCCCACCGTCTACGACTGGCTCTACAGCGGGGAGACCAGGAGCATCCATCATCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Auxin_repressed

Protein Analysis

117

Amino Acids

12.63

Weight (kDa)

9.63

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_repressed PF05564 7 - 115 2.1e-49 Dormancy/auxin associated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 158
AccBSI CCGCTC 1 cut(s) 191
AccI GTMKAC 1 cut(s) 303
AciI CCGC 2 cut(s) 189, 321
AclI AACGTT 1 cut(s) 207
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 2 cut(s) 14, 97
AjnI CCWGG 5 cut(s) 154, 163, 229, 264, 329
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AluBI AGCT 2 cut(s) 19, 185
AluI AGCT 2 cut(s) 19, 185
Alw26I GTCTC 2 cut(s) 87, 320
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 1 cut(s) 58
AspS9I GGNCC 1 cut(s) 41
AsuC2I CCSGG 3 cut(s) 147, 173, 174
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 179
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 146
BauI CACGAG 1 cut(s) 192
BbvCI CCTCAGC 1 cut(s) 45
BccI CCATC 2 cut(s) 234, 348
BceAI ACGGC 1 cut(s) 64
BciT130I CCWGG 5 cut(s) 156, 165, 231, 266, 331
BcnI CCSGG 3 cut(s) 147, 173, 174
BcoDI GTCTC 2 cut(s) 87, 320
BfmI CTRYAG 1 cut(s) 316
Bme1390I CCNGG 8 cut(s) 147, 156, 165, 173, 174, 231, 266, 331
Bme18I GGWCC 1 cut(s) 41
BmeT110I CYCGRG 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 41
BmiI GGNNCC 2 cut(s) 160, 169
BmrFI CCNGG 8 cut(s) 147, 156, 165, 173, 174, 231, 266, 331
BmsI GCATC 2 cut(s) 123, 345
Bpu10I CCTNAGC 4 cut(s) 45, 71, 87, 198
BpuMI CCSGG 3 cut(s) 147, 173, 174
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 6 cut(s) 61, 145, 163, 171, 172, 255
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 314
BseBI CCWGG 5 cut(s) 156, 165, 231, 266, 331
BseDI CCNNGG 6 cut(s) 61, 145, 163, 171, 172, 255
BseGI GGATG 3 cut(s) 31, 226, 336
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 59
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 1 cut(s) 229
BseSI GKGCMC 1 cut(s) 179
BshFI GGCC 1 cut(s) 60
BshNI GGYRCC 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 3 cut(s) 39, 147, 173
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 2 cut(s) 87, 320
BsmBI CGTCTC 1 cut(s) 87
BsnI GGCC 1 cut(s) 60
Bso31I GGTCTC 1 cut(s) 320
BsoBI CYCGRG 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 146, 179
Bsp19I CCATGG 1 cut(s) 255
BspACI CCGC 2 cut(s) 189, 321
BspANI GGCC 1 cut(s) 60
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 2 cut(s) 160, 169
BspT107I GGYRCC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 320
BsrBI CCGCTC 1 cut(s) 191
BsrI ACTGG 1 cut(s) 314
BssECI CCNNGG 6 cut(s) 61, 145, 163, 171, 172, 255
BssSI CACGAG 1 cut(s) 192
BssT1I CCWWGG 2 cut(s) 61, 255
Bst2BI CACGAG 1 cut(s) 192
Bst2UI CCWGG 5 cut(s) 156, 165, 231, 266, 331
Bst4CI ACNGT 1 cut(s) 301
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 4 cut(s) 45, 71, 87, 198
BstDSI CCRYGG 1 cut(s) 255
BstF5I GGATG 3 cut(s) 31, 226, 336
BstMAI GTCTC 2 cut(s) 87, 320
BstMWI GCNNNNNNNGC 2 cut(s) 16, 76
BstNI CCWGG 5 cut(s) 156, 165, 231, 266, 331
BstNSI RCATGY 1 cut(s) 70
BstSCI CCNGG 8 cut(s) 145, 154, 163, 171, 172, 229, 264, 329
BstSFI CTRYAG 1 cut(s) 316
BstSLI GKGCMC 1 cut(s) 179
BstXI CCANNNNNNTGG 1 cut(s) 256
BsuRI GGCC 1 cut(s) 60
BtgI CCRYGG 1 cut(s) 255
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 3 cut(s) 31, 226, 336
Cac8I GCNNGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 41
Cfr9I CCCGGG 1 cut(s) 172
CsiI ACCWGGT 1 cut(s) 264
CviAII CATG 2 cut(s) 67, 256
DdeI CTNAG 4 cut(s) 45, 71, 87, 198
Eco130I CCWWGG 2 cut(s) 61, 255
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 41
Eco88I CYCGRG 1 cut(s) 172
EcoRII CCWGG 5 cut(s) 154, 163, 229, 264, 329
EcoT14I CCWWGG 2 cut(s) 61, 255
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 61, 255
Esp3I CGTCTC 1 cut(s) 87
FaeI CATG 2 cut(s) 70, 259
FaiI YATR 2 cut(s) 68, 257
FatI CATG 2 cut(s) 66, 255
FauI CCCGC 1 cut(s) 314
FblI GTMKAC 1 cut(s) 303
FokI GGATG 3 cut(s) 38, 213, 323
FriOI GRGCYC 1 cut(s) 146
HaeIII GGCC 1 cut(s) 60
HapII CCGG 3 cut(s) 39, 147, 173
Hin1II CATG 2 cut(s) 70, 259
HindIII AAGCTT 1 cut(s) 17
HinfI GANTC 1 cut(s) 116
HpaII CCGG 3 cut(s) 39, 147, 173
Hpy166II GTNNAC 1 cut(s) 304
Hpy8I GTNNAC 1 cut(s) 304
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4IV ACGT 2 cut(s) 55, 207
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 76
HpyF3I CTNAG 4 cut(s) 45, 71, 87, 198
HpySE526I ACGT 2 cut(s) 55, 207
Hsp92II CATG 2 cut(s) 70, 259
LmnI GCTCC 1 cut(s) 333
LweI GCATC 2 cut(s) 123, 345
MabI ACCWGGT 1 cut(s) 264
MaeII ACGT 2 cut(s) 55, 207
MbiI CCGCTC 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 146, 179
MlyI GAGTC 1 cut(s) 125
MnlI CCTC 3 cut(s) 54, 103, 207
MseI TTAA 1 cut(s) 349
MspA1I CMGCKG 1 cut(s) 321
MspI CCGG 3 cut(s) 39, 147, 173
MspR9I CCNGG 8 cut(s) 147, 156, 165, 173, 174, 231, 266, 331
MvaI CCWGG 5 cut(s) 156, 165, 231, 266, 331
MwoI GCNNNNNNNGC 2 cut(s) 16, 76
NciI CCSGG 3 cut(s) 147, 173, 174
NcoI CCATGG 1 cut(s) 255
NlaIII CATG 2 cut(s) 70, 259
NlaIV GGNNCC 2 cut(s) 160, 169
NspI RCATGY 1 cut(s) 70
PaeI GCATGC 1 cut(s) 70
PleI GAGTC 1 cut(s) 124
PpsI GAGTC 1 cut(s) 124
Psp1406I AACGTT 1 cut(s) 207
Psp6I CCWGG 5 cut(s) 154, 163, 229, 264, 329
PspGI CCWGG 5 cut(s) 154, 163, 229, 264, 329
PspN4I GGNNCC 2 cut(s) 160, 169
PspPI GGNCC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 349
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 8 cut(s) 147, 156, 165, 173, 174, 231, 266, 331
SduI GDGCHC 2 cut(s) 146, 179
SetI ASST 8 cut(s) 21, 46, 58, 187, 210, 235, 243, 270
SexAI ACCWGGT 1 cut(s) 264
SfaNI GCATC 2 cut(s) 123, 345
SfcI CTRYAG 1 cut(s) 316
SinI GGWCC 1 cut(s) 41
SmaI CCCGGG 1 cut(s) 174
SphI GCATGC 1 cut(s) 70
SsiI CCGC 2 cut(s) 189, 321
StyD4I CCNGG 8 cut(s) 145, 154, 163, 171, 172, 229, 264, 329
StyI CCWWGG 2 cut(s) 61, 255
TaaI ACNGT 1 cut(s) 301
TaiI ACGT 2 cut(s) 58, 210
TaqI TCGA 2 cut(s) 119, 273
Tru1I TTAA 1 cut(s) 349
Tru9I TTAA 1 cut(s) 349
TspMI CCCGGG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 41
XceI RCATGY 1 cut(s) 70
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XmaI CCCGGG 1 cut(s) 172
XmiI GTMKAC 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.