pycom05g29370

Wings apart-like protein regulation of heterochromatin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0009354
Physical Location & Seq
Forward (+)
29810088 .. 29815978
5891 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3057 bp
ATGATCAAGAAGAATGTGATTGGGGCAGCCCAAGGAATTCAAAACCAAACCAAATCAAATCGAAACCCGAACCAAAAACCCCCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTTCGACCCAATTTCTCTCTCTCCTCAAATTGGACAACAGACCTCTCCTCCTTCTTCTTCACCTGAAGTACCAGTCTTCCAGACCTAGAATTTTCCAATTTCGCCCTTCAGTTTCAAATCGTGATTCCCCCAAAACGAAAAGCCTTGGCTTTTCTATTCAGACATGCCCGTCCCGAACCGATAACGCAGAGGAAGGAACCCAGCCACAACCAAAACGCTGCCGTTTACCCAAGATGATCGTGAGAACGTACGGCCGTCGCAAGGGAGGGGACATTACCAGAACGTATTCCGACACAGAGCTAAACGACGCCGTTCACGACGACGACGACGTCAGCAGCGACCCCTTCGGATTTTCCCTATCGCAGCCTCAAGAAAGAAGCGGCGACCCTTTCAATTTCTCGTCTCAGGAGGACTCGTCTTCCGGGTGGGCCCACTTCGATTCGGACCCTTACGTCACCAAGGACTCCTCCTTGAAGCGCTTTCCGCTGGACGGCGTCGTCTCGGGGAGGTCCAAGAAAGCTAAGACTCGGAAGGAGGCGGTGGGGAAGAACTCCTACAGGCCGCCGCCGACGCCGTCTATTCTTGCGACATCGACCTTGATGGAGGCGCAGGAGTTTGGGGAGATGATGGAGCACGTGGACGAGGTCAATTTCGCGCTGGATGGTCTCCGGAAGGGTCAGCCAGTTCGGATCAGAAGGGCTAGCTTGCTGTCGTTGCTGTCTATTTGCGGCACGGCGCAGCAGAGACGGCTTCTCAGGACTCAGGGGATGGCAAAAACTATAATCGAGGCTATATTGGGTCTCAGCTTTGATGATTCACCCAGCAATCTGGCTGCTGCAACTATTTTCTATGTTTTGACAAGTGATGGTCAGGATGATCGTCTCCTGGAATCACCGAACTGTATTAATTTTTTGATTAGGTTTTGCAAACCAATTGTCTCTAACACCACAGAAGATAAAGTACCAAAGGTTGGACGTAAGCTTCTAGCATTGCGTATTGGTGCTGACATCTCGCAGTGTACGACAAAAAGATTGGACTCCAGCTCTGCCGCTATTTTTTCCAAAGTTCAAGAAATTCTTGTAGGTTGCAAGGAATTGAAACCAAGTTGTGGGGATGAAGGAGAAATGGAAAATCCGGAGTTATGCCCAAAATGGATAGCTTTGCTGACTATGGAGAAAGCTTGCTTATCTACTATCTCTCTTGAAGAAACCACTGGTGCAGTAAGGAAGTCGGGGGGCAACTTTAAGGAAAAATTACGAGAGCTTGGAGGTCTTGATGCTGTATTTGAGGTGACCATGGGTTGCCATTCCAATATGGAGGGTTGGCTAAAAGATAGCTCGCATACTATTTGGGAAAATGAAAAACACATGGTGCGGAGCCTGGTTCTGCTTTTGAAATGTTTGAAGATCATGGAAAATGCCACATTCCTAAGTAAAGAGAATCAGAGCCATTTGCTAGGAATGAAAAGGAAGTTGGATCCCATGGCAAATCCTTTGTCTTTTACAGAACTAGTTATAAGTGCCATCAATATTCTGTCAGGAATTTGTCTACATAAAAGTTCCTCTTCTGCCACCAATGACAAAAAGTCTTATAGTCTTTCTAATGGGAGTGAACATGTTTCTGAGAAGAGCTCCAATAGATGTCAGAGCAGTCGGTTAATGTCAACACCTTGCTCGGTATATACTATATCCAGTTCGGAAACTACCAGCACCTCCATGACTGATACTTATTCATTGAACACAAGACTCAATTCTTCTAGAAATGGTTCTTCCAATGGTACATCAAGACATGTCAGCGGTGAAACCGGTAAATTTAGCAATCTTTCCCTGAAGAATGCTGGTCTCAGTCAGCGATCCTATAACTTTGAAGACTCCAAAATTGATCTTTCAGAGGATAGTCAAGATCCTTTCGCATTTAGCGATGGTTCCAGAATGGATGCTGATCTCAGTCAGAGATCCTATATTTCCGAAGACACCAAAATTGATCTTTCACGGGAGCGTCAAGATCCTTTTGCATTTGATGAAGATGATTATAAACCTTCTAAGTGGGACATGTTATCTGGCAAGACAACTATGTCTCTATCGCAACAGAATGCGGCAGCATACAGAGAAATTGATGACATTTGTCAGTTGCAGCATATTATGAGCCAAGAAGCATCAAGTAATGGAGAAAACCATCAAACACAAGAGACATCCTGTTCAGGTGCTGTTAGCCAAGAAGGTTCCAGTCTTGTAGCTGAATGCCTTCTTACTGCTGTCAAGGTTCTTATGAACTTAGCAAATGATAATCCTGTTGGGTGTCAGCAAATTGCTGCATATGGAGGGCTGGAAACCTTGTCTTCTTTGATTGCCAACCACTTTCCTTGTTTCAACTCATCATCATCTCCCTTCAGTGAGAGAAGTGAGAATATTTCCAGCATTGAACTTGACCATCAGAATAATAGACATCTTACTGATCAAGAGCTGGACTTTCTTGTTGCTATTTTGGGTCTGCTTGTGAACTTGGTGGAGAAAGATGGCCAAAACAGATCACGGCTTGCAGCAGCTAGTGTTCAGGTAGCTAGTTCGGAAGGGTTTGGAGAGGAGAGTTGCAAGGATCTAATTCTGCTAATCTGCTCTATCTTTCTTGCGAACCAAGGTGCAGGCGAGGGAGGTGGAGAAGAAATGGTTTTGCCAACTGATGAGGCAGCTGTCTTACAAGGGGAACAGGAAGCAGAGAAAATGATAATAGAAGCTTATTCTGCCCTGCTTCTAGCATTTCTTTCAACCGAAAGTAAGAACATACGTGATGCAATTTCTGACTGTCTCCCAGACCGCAACCTGGCTATTCTAGTACCTGTGCTGGACAGATTTGTGGCATTTCATTTGACGCTGAACATGATATCGCCCGAGACTCATAAAGCTGTGAGTGAAGTAATCGAATCATGTAGGATGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WAPL

Protein Analysis

1019

Amino Acids

111.9

Weight (kDa)

5.68

Isoelectric Point (pI)

50.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WAPL PF07814 237 - 565 6.8e-45 Wings apart-like protein regulation of heterochromatin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1634, 2151
AasI GACNNNNNNGTC 5 cut(s) 286, 446, 569, 614, 2191
AatII GACGTC 1 cut(s) 450
AccB7I CCANNNNNTGG 2 cut(s) 1088, 1226
AccI GTMKAC 1 cut(s) 1666
AccII CGCG 1 cut(s) 773
AccIII TCCGGA 2 cut(s) 786, 1252
AclWI GGATC 8 cut(s) 815, 1589, 1602, 1965, 2015, 2067, 2117, 2721
AcoI YGGCCR 2 cut(s) 370, 2635
AcsI RAATTY 5 cut(s) 36, 207, 1191, 1659, 1928
AcuI CTGAAG 4 cut(s) 203, 210, 1967, 2491
AcvI CACGTG 1 cut(s) 754
AcyI GRCGYC 4 cut(s) 426, 447, 612, 689
AdeI CACNNNGTG 1 cut(s) 1489
AfaI GTAC 6 cut(s) 188, 368, 1080, 1138, 1897, 2952
AfeI AGCGCT 1 cut(s) 596
AfiI CCNNNNNNNGG 6 cut(s) 148, 379, 608, 1088, 1226, 2938
AflIII ACRYGT 3 cut(s) 1732, 1906, 2169
AgeI ACCGGT 1 cut(s) 1922
AhdI GACNNNNNGTC 1 cut(s) 1702
AhlI ACTAGT 1 cut(s) 1627
AjnI CCWGG 3 cut(s) 1002, 1497, 2937
AjuI GAANNNNNNNTTGG 2 cut(s) 1574, 1606
Alw21I GWGCWC 2 cut(s) 753, 1751
AlwI GGATC 8 cut(s) 815, 1589, 1602, 1965, 2015, 2067, 2117, 2721
AlwNI CAGNNNCTG 1 cut(s) 2324
Ama87I CYCGRG 2 cut(s) 619, 3005
Aor13HI TCCGGA 2 cut(s) 786, 1252
Aor51HI AGCGCT 1 cut(s) 596
AoxI GGCC 4 cut(s) 370, 546, 677, 2635
ApaI GGGCCC 1 cut(s) 550
ApoI RAATTY 5 cut(s) 36, 207, 1191, 1659, 1928
ArsI GACNNNNNNTTYG 4 cut(s) 1038, 1070, 1598, 1630
AseI ATTAAT 1 cut(s) 1023
AsiGI ACCGGT 1 cut(s) 1922
Asp700I GAANNNNTTC 3 cut(s) 403, 1882, 2361
AspLEI GCGC 4 cut(s) 597, 727, 775, 856
AspS9I GGNCC 4 cut(s) 546, 547, 562, 627
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 6 cut(s) 170, 565, 927, 1002, 1420, 1928
AsuNHI GCTAGC 1 cut(s) 818
AvaI CYCGRG 2 cut(s) 619, 3005
AvaII GGWCC 2 cut(s) 562, 627
BaeGI GKGCMC 1 cut(s) 550
BalI TGGCCA 1 cut(s) 2637
BamHI GGATCC 1 cut(s) 1594
BanII GRGCYC 2 cut(s) 550, 1751
BarI GAAGNNNNNNTAC 4 cut(s) 656, 688, 1062, 1094
BbrPI CACGTG 1 cut(s) 754
BbsI GAAGAC 5 cut(s) 186, 528, 1992, 2094, 2448
Bbv12I GWGCWC 2 cut(s) 753, 1751
BceAI ACGGC 9 cut(s) 324, 357, 385, 413, 625, 676, 867, 881, 2666
BciT130I CCWGG 3 cut(s) 1004, 1499, 2939
BclI TGATCA 2 cut(s) 3, 2572
BcnI CCSGG 1 cut(s) 541
BcuI ACTAGT 1 cut(s) 1627
BfmI CTRYAG 1 cut(s) 673
BfoI RGCGCY 1 cut(s) 598
Bme1390I CCNGG 4 cut(s) 541, 1004, 1499, 2939
Bme18I GGWCC 2 cut(s) 562, 627
BmeRI GACNNNNNGTC 1 cut(s) 1702
BmeT110I CYCGRG 2 cut(s) 619, 3005
BmgT120I GGNCC 4 cut(s) 546, 547, 562, 627
BmiI GGNNCC 7 cut(s) 316, 548, 564, 1496, 1596, 2044, 2341
BmrFI CCNGG 4 cut(s) 541, 1004, 1499, 2939
BmsI GCATC 5 cut(s) 1384, 2044, 2282, 2896, 3039
BmtI GCTAGC 1 cut(s) 822
BoxI GACNNNNGTC 1 cut(s) 2241
BpiI GAAGAC 5 cut(s) 186, 528, 1992, 2094, 2448
BplI GAGNNNNNCTC 2 cut(s) 1733, 1765
BpmI CTGGAG 1 cut(s) 1141
BpuEI CTTGAG 1 cut(s) 471
BpuMI CCSGG 1 cut(s) 541
BsaAI YACGTR 2 cut(s) 754, 2903
BsaBI GATNNNNATC 1 cut(s) 2058
BsaHI GRCGYC 4 cut(s) 426, 447, 612, 689
BsaI GGTCTC 3 cut(s) 788, 923, 1964
BsaJI CCNNGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
BsaWI WCCGGW 3 cut(s) 786, 1252, 1922
BsaXI ACNNNNNCTCC 4 cut(s) 150, 180, 518, 548
Bsc4I CCNNNNNNNGG 6 cut(s) 148, 379, 608, 1088, 1226, 2938
Bse118I RCCGGY 1 cut(s) 1922
Bse1I ACTGG 5 cut(s) 190, 800, 1336, 1809, 2343
Bse3DI GCAATG 1 cut(s) 1106
Bse8I GATNNNNATC 1 cut(s) 2058
BseAI TCCGGA 2 cut(s) 786, 1252
BseBI CCWGG 3 cut(s) 1004, 1499, 2939
BseDI CCNNGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
BseGI GGATG 7 cut(s) 784, 891, 997, 1237, 2059, 2309, 3054
BseJI GATNNNNATC 1 cut(s) 2058
BseLI CCNNNNNNNGG 6 cut(s) 148, 379, 608, 1088, 1226, 2938
BseMI GCAATG 1 cut(s) 1106
BseMII CTCAG 7 cut(s) 536, 886, 893, 934, 1731, 1975, 2077
BseNI ACTGG 5 cut(s) 190, 800, 1336, 1809, 2343
BseRI GAGGAG 5 cut(s) 106, 131, 155, 574, 2714
BseSI GKGCMC 1 cut(s) 550
BseX3I CGGCCG 1 cut(s) 370
BseYI CCCAGC 2 cut(s) 318, 938
BsgI GTGCAG 2 cut(s) 1356, 2778
Bsh1236I CGCG 1 cut(s) 773
Bsh1285I CGRYCG 1 cut(s) 373
BshFI GGCC 4 cut(s) 372, 548, 679, 2637
BshTI ACCGGT 1 cut(s) 1922
BsiEI CGRYCG 1 cut(s) 373
BsiHKAI GWGCWC 2 cut(s) 753, 1751
BsiHKCI CYCGRG 2 cut(s) 619, 3005
BsiSI CCGG 4 cut(s) 540, 787, 1253, 1923
BsiWI CGTACG 1 cut(s) 366
BslFI GGGAC 3 cut(s) 274, 401, 2180
BslI CCNNNNNNNGG 6 cut(s) 148, 379, 608, 1088, 1226, 2938
BsmBI CGTCTC 4 cut(s) 525, 622, 856, 1004
BsmFI GGGAC 3 cut(s) 274, 401, 2180
BsmI GAATGC 3 cut(s) 1957, 2215, 2363
BsnI GGCC 4 cut(s) 372, 548, 679, 2637
Bso31I GGTCTC 3 cut(s) 788, 923, 1964
BsoBI CYCGRG 2 cut(s) 619, 3005
Bsp120I GGGCCC 1 cut(s) 546
Bsp1286I GDGCHC 3 cut(s) 550, 753, 1751
Bsp13I TCCGGA 2 cut(s) 786, 1252
Bsp19I CCATGG 2 cut(s) 1413, 1599
BspANI GGCC 4 cut(s) 372, 548, 679, 2637
BspCNI CTCAG 7 cut(s) 535, 885, 892, 933, 1732, 1974, 2076
BspEI TCCGGA 2 cut(s) 786, 1252
BspFNI CGCG 1 cut(s) 773
BspLI GGNNCC 7 cut(s) 316, 548, 564, 1496, 1596, 2044, 2341
BspOI GCTAGC 1 cut(s) 822
BspPI GGATC 8 cut(s) 815, 1589, 1602, 1965, 2015, 2067, 2117, 2721
BspQI GCTCTTC 1 cut(s) 1739
BspTNI GGTCTC 3 cut(s) 788, 923, 1964
BsrDI GCAATG 1 cut(s) 1106
BsrFI RCCGGY 1 cut(s) 1922
BsrI ACTGG 5 cut(s) 190, 800, 1336, 1809, 2343
BssAI RCCGGY 1 cut(s) 1922
BssECI CCNNGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
BssNI GRCGYC 4 cut(s) 426, 447, 612, 689
BssT1I CCWWGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
Bst2UI CCWGG 3 cut(s) 1004, 1499, 2939
Bst4CI ACNGT 2 cut(s) 1019, 2921
Bst6I CTCTTC 3 cut(s) 124, 1687, 1739
BstACI GRCGYC 4 cut(s) 426, 447, 612, 689
BstBAI YACGTR 2 cut(s) 754, 2903
BstC8I GCNNGC 6 cut(s) 820, 824, 1300, 1457, 2655, 2761
BstDSI CCRYGG 2 cut(s) 1413, 1599
BstEII GGTNACC 1 cut(s) 1408
BstF5I GGATG 7 cut(s) 784, 891, 997, 1237, 2059, 2309, 3054
BstFNI CGCG 1 cut(s) 773
BstH2I RGCGCY 1 cut(s) 598
BstHHI GCGC 4 cut(s) 597, 727, 775, 856
BstMCI CGRYCG 1 cut(s) 373
BstMWI GCNNNNNNNGC 5 cut(s) 601, 688, 832, 865, 2858
BstNI CCWGG 3 cut(s) 1004, 1499, 2939
BstNSI RCATGY 4 cut(s) 285, 1736, 1910, 2173
BstPAI GACNNNNGTC 1 cut(s) 2241
BstPI GGTNACC 1 cut(s) 1408
BstSCI CCNGG 4 cut(s) 539, 1002, 1497, 2937
BstSFI CTRYAG 1 cut(s) 673
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 1 cut(s) 773
BstV2I GAAGAC 5 cut(s) 186, 528, 1992, 2094, 2448
BstX2I RGATCY 5 cut(s) 1594, 2020, 2072, 2122, 2713
BstXI CCANNNNNNTGG 1 cut(s) 946
BstYI RGATCY 5 cut(s) 1594, 2020, 2072, 2122, 2713
BstZI CGGCCG 1 cut(s) 370
BsuRI GGCC 4 cut(s) 372, 548, 679, 2637
BtgI CCRYGG 2 cut(s) 1413, 1599
BtgZI GCGATG 1 cut(s) 2052
BtsCI GGATG 7 cut(s) 784, 891, 997, 1237, 2059, 2309, 3054
BtsI GCAGTG 1 cut(s) 1139
BtsIMutI CAGTG 3 cut(s) 1139, 1329, 2515
Cac8I GCNNGC 6 cut(s) 820, 824, 1300, 1457, 2655, 2761
CaiI CAGNNNCTG 1 cut(s) 2324
CfoI GCGC 4 cut(s) 597, 727, 775, 856
Cfr10I RCCGGY 1 cut(s) 1922
Cfr13I GGNCC 4 cut(s) 546, 547, 562, 627
CseI GACGC 5 cut(s) 434, 601, 697, 2105, 2995
Csp6I GTAC 6 cut(s) 187, 367, 1079, 1137, 1896, 2951
CspAI ACCGGT 1 cut(s) 1922
CspCI CAANNNNNGTGG 2 cut(s) 2462, 2497
CviQI GTAC 6 cut(s) 187, 367, 1079, 1137, 1896, 2951
DraIII CACNNNGTG 1 cut(s) 1489
DrdI GACNNNNNNGTC 5 cut(s) 286, 446, 569, 614, 2191
DriI GACNNNNNGTC 1 cut(s) 1702
DseDI GACNNNNNNGTC 5 cut(s) 286, 446, 569, 614, 2191
EaeI YGGCCR 2 cut(s) 370, 2635
EagI CGGCCG 1 cut(s) 370
Eam1104I CTCTTC 3 cut(s) 124, 1687, 1739
Eam1105I GACNNNNNGTC 1 cut(s) 1702
EarI CTCTTC 3 cut(s) 124, 1687, 1739
Ecl136II GAGCTC 1 cut(s) 1749
EclXI CGGCCG 1 cut(s) 370
Eco130I CCWWGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
Eco24I GRGCYC 2 cut(s) 550, 1751
Eco31I GGTCTC 3 cut(s) 788, 923, 1964
Eco32I GATATC 1 cut(s) 3000
Eco47I GGWCC 2 cut(s) 562, 627
Eco47III AGCGCT 1 cut(s) 596
Eco52I CGGCCG 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 1749
Eco57I CTGAAG 4 cut(s) 203, 210, 1967, 2491
Eco72I CACGTG 1 cut(s) 754
Eco88I CYCGRG 2 cut(s) 619, 3005
Eco91I GGTNACC 1 cut(s) 1408
EcoICRI GAGCTC 1 cut(s) 1749
EcoO65I GGTNACC 1 cut(s) 1408
EcoRI GAATTC 1 cut(s) 36
EcoRII CCWGG 3 cut(s) 1002, 1497, 2937
EcoRV GATATC 1 cut(s) 3000
EcoT14I CCWWGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
EcoT38I GRGCYC 2 cut(s) 550, 1751
ErhI CCWWGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
Esp3I CGTCTC 4 cut(s) 525, 622, 856, 1004
FalI AAGNNNNNCTT 4 cut(s) 1179, 1211, 1666, 1698
FaqI GGGAC 3 cut(s) 274, 401, 2180
FauNDI CATATG 1 cut(s) 2434
FbaI TGATCA 2 cut(s) 3, 2572
FblI GTMKAC 1 cut(s) 1666
FokI GGATG 6 cut(s) 791, 898, 1004, 1244, 2066, 2296
FriOI GRGCYC 2 cut(s) 550, 1751
GlaI GCGC 4 cut(s) 596, 726, 774, 855
GsaI CCCAGC 2 cut(s) 322, 942
GsuI CTGGAG 1 cut(s) 1141
HaeII RGCGCY 1 cut(s) 598
HaeIII GGCC 4 cut(s) 372, 548, 679, 2637
HapII CCGG 4 cut(s) 540, 787, 1253, 1923
HgaI GACGC 5 cut(s) 434, 601, 697, 2105, 2995
HhaI GCGC 4 cut(s) 597, 727, 775, 856
Hin1I GRCGYC 4 cut(s) 426, 447, 612, 689
Hin6I GCGC 4 cut(s) 595, 725, 773, 854
HinP1I GCGC 4 cut(s) 595, 725, 773, 854
HincII GTYRAC 1 cut(s) 1782
HindII GTYRAC 1 cut(s) 1782
HindIII AAGCTT 3 cut(s) 1097, 1296, 2850
HpaII CCGG 4 cut(s) 540, 787, 1253, 1923
HphI GGTGA 6 cut(s) 170, 565, 927, 1002, 1420, 1928
Hpy166II GTNNAC 8 cut(s) 344, 433, 757, 1137, 1667, 1730, 1782, 2617
Hpy8I GTNNAC 8 cut(s) 344, 433, 757, 1137, 1667, 1730, 1782, 2617
Hpy99I CGWCG 8 cut(s) 378, 428, 440, 443, 446, 449, 617, 691
HpyCH4III ACNGT 2 cut(s) 1019, 2921
HpyCH4IV ACGT 7 cut(s) 365, 401, 447, 570, 753, 1093, 2902
HpyF10VI GCNNNNNNNGC 5 cut(s) 601, 688, 832, 865, 2858
HpySE526I ACGT 7 cut(s) 365, 401, 447, 570, 753, 1093, 2902
Hsp92I GRCGYC 4 cut(s) 426, 447, 612, 689
HspAI GCGC 4 cut(s) 595, 725, 773, 854
Kpn2I TCCGGA 2 cut(s) 786, 1252
Ksp22I TGATCA 2 cut(s) 3, 2572
LguI GCTCTTC 1 cut(s) 1739
LmnI GCTCC 4 cut(s) 748, 1494, 1754, 2113
LweI GCATC 5 cut(s) 1384, 2044, 2282, 2896, 3039
MaeII ACGT 7 cut(s) 365, 401, 447, 570, 753, 1093, 2902
MaeIII GTNAC 2 cut(s) 571, 1408
MfeI CAATTG 1 cut(s) 1050
MflI RGATCY 5 cut(s) 1594, 2020, 2072, 2122, 2713
MhlI GDGCHC 3 cut(s) 550, 753, 1751
MlsI TGGCCA 1 cut(s) 2637
MluNI TGGCCA 1 cut(s) 2637
MlyI GAGTC 8 cut(s) 524, 575, 637, 871, 1148, 1857, 1982, 3004
MmeI TCCRAC 3 cut(s) 432, 1069, 1572
Mox20I TGGCCA 1 cut(s) 2637
MroI TCCGGA 2 cut(s) 786, 1252
MroXI GAANNNNTTC 3 cut(s) 403, 1882, 2361
MscI TGGCCA 1 cut(s) 2637
MseI TTAA 3 cut(s) 1023, 1362, 1775
MslI CAYNNNNRTG 1 cut(s) 1832
Msp20I TGGCCA 1 cut(s) 2637
MspA1I CMGCKG 3 cut(s) 604, 1914, 2807
MspI CCGG 4 cut(s) 540, 787, 1253, 1923
MspR9I CCNGG 4 cut(s) 541, 1004, 1499, 2939
MunI CAATTG 1 cut(s) 1050
Mva1269I GAATGC 3 cut(s) 1957, 2215, 2363
MvaI CCWGG 3 cut(s) 1004, 1499, 2939
MvnI CGCG 1 cut(s) 773
MwoI GCNNNNNNNGC 5 cut(s) 601, 688, 832, 865, 2858
NciI CCSGG 1 cut(s) 541
NcoI CCATGG 2 cut(s) 1413, 1599
NdeI CATATG 1 cut(s) 2434
NheI GCTAGC 1 cut(s) 818
NlaIV GGNNCC 7 cut(s) 316, 548, 564, 1496, 1596, 2044, 2341
NmuCI GTSAC 2 cut(s) 571, 1408
NspI RCATGY 4 cut(s) 285, 1736, 1910, 2173
PciI ACATGT 3 cut(s) 1732, 1906, 2169
PciSI GCTCTTC 1 cut(s) 1739
PcsI WCGNNNNNNNCGW 6 cut(s) 432, 441, 444, 453, 1136, 2034
PctI GAATGC 3 cut(s) 1957, 2215, 2363
PdmI GAANNNNTTC 3 cut(s) 403, 1882, 2361
PfeI GAWTC 6 cut(s) 242, 557, 932, 1007, 1558, 3038
Pfl23II CGTACG 1 cut(s) 366
PflFI GACNNNGTC 4 cut(s) 446, 611, 691, 761
PflMI CCANNNNNTGG 2 cut(s) 1088, 1226
PfoI TCCNGGA 1 cut(s) 1002
PinAI ACCGGT 1 cut(s) 1922
PleI GAGTC 8 cut(s) 524, 575, 637, 871, 1148, 1857, 1982, 3004
PmaCI CACGTG 1 cut(s) 754
PmlI CACGTG 1 cut(s) 754
PpsI GAGTC 8 cut(s) 524, 575, 637, 871, 1148, 1857, 1982, 3004
Ppu21I YACGTR 2 cut(s) 754, 2903
PscI ACATGT 3 cut(s) 1732, 1906, 2169
PshAI GACNNNNGTC 1 cut(s) 2241
PshBI ATTAAT 1 cut(s) 1023
PsiI TTATAA 2 cut(s) 1634, 2151
Psp124BI GAGCTC 1 cut(s) 1751
Psp6I CCWGG 3 cut(s) 1002, 1497, 2937
PspCI CACGTG 1 cut(s) 754
PspEI GGTNACC 1 cut(s) 1408
PspFI CCCAGC 2 cut(s) 318, 938
PspGI CCWGG 3 cut(s) 1002, 1497, 2937
PspLI CGTACG 1 cut(s) 366
PspN4I GGNNCC 7 cut(s) 316, 548, 564, 1496, 1596, 2044, 2341
PspOMI GGGCCC 1 cut(s) 546
PspPI GGNCC 4 cut(s) 546, 547, 562, 627
PstNI CAGNNNCTG 1 cut(s) 2324
PsuI RGATCY 5 cut(s) 1594, 2020, 2072, 2122, 2713
PsyI GACNNNGTC 4 cut(s) 446, 611, 691, 761
PvuII CAGCTG 1 cut(s) 2807
RsaI GTAC 6 cut(s) 188, 368, 1080, 1138, 1897, 2952
RsaNI GTAC 6 cut(s) 187, 367, 1079, 1137, 1896, 2951
RseI CAYNNNNRTG 1 cut(s) 1832
SacI GAGCTC 1 cut(s) 1751
SapI GCTCTTC 1 cut(s) 1739
SaqAI TTAA 3 cut(s) 1023, 1362, 1775
Sau96I GGNCC 4 cut(s) 546, 547, 562, 627
SchI GAGTC 8 cut(s) 524, 575, 637, 871, 1148, 1857, 1982, 3004
ScrFI CCNGG 4 cut(s) 541, 1004, 1499, 2939
SduI GDGCHC 3 cut(s) 550, 753, 1751
SfaNI GCATC 5 cut(s) 1384, 2044, 2282, 2896, 3039
SfcI CTRYAG 1 cut(s) 673
SinI GGWCC 2 cut(s) 562, 627
SmiMI CAYNNNNRTG 1 cut(s) 1832
SmlI CTYRAG 1 cut(s) 486
SmoI CTYRAG 1 cut(s) 486
SpeI ACTAGT 1 cut(s) 1627
SspI AATATT 2 cut(s) 1648, 2527
SstI GAGCTC 1 cut(s) 1751
StyD4I CCNGG 4 cut(s) 539, 1002, 1497, 2937
StyI CCWWGG 6 cut(s) 31, 262, 576, 1413, 1599, 2752
TaaI ACNGT 2 cut(s) 1019, 2921
TaiI ACGT 7 cut(s) 368, 404, 450, 573, 756, 1096, 2905
TaqI TCGA 6 cut(s) 61, 122, 555, 710, 903, 3036
TauI GCSGC 6 cut(s) 501, 682, 685, 849, 1169, 2216
TfiI GAWTC 6 cut(s) 242, 557, 932, 1007, 1558, 3038
Tru1I TTAA 3 cut(s) 1023, 1362, 1775
Tru9I TTAA 3 cut(s) 1023, 1362, 1775
TscAI CASTG 3 cut(s) 1139, 1336, 2515
TseFI GTSAC 2 cut(s) 571, 1408
Tsp45I GTSAC 2 cut(s) 571, 1408
TspDTI ATGAA 7 cut(s) 1248, 1491, 1595, 1839, 2154, 2402, 2969
TspRI CASTG 3 cut(s) 1139, 1336, 2515
Tth111I GACNNNGTC 4 cut(s) 446, 611, 691, 761
Van91I CCANNNNNTGG 2 cut(s) 1088, 1226
VpaK11BI GGWCC 2 cut(s) 562, 627
VspI ATTAAT 1 cut(s) 1023
XapI RAATTY 5 cut(s) 36, 207, 1191, 1659, 1928
XbaI TCTAGA 1 cut(s) 1874
XceI RCATGY 4 cut(s) 285, 1736, 1910, 2173
XmiI GTMKAC 1 cut(s) 1666
XmnI GAANNNNTTC 3 cut(s) 403, 1882, 2361
ZraI GACGTC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.