pycom05g30040
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0015711
Physical Location & Seq
Forward (+)
30291753 .. 30292534
782 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30040.1

Sequence Viewer

Length: 555 bp
ATGGTTTCAATCTATGGTGTTGACAAGTGGGGAAGAAGGTTCCTTTTCCTTGAGGGTGGAGCTCAAATGCTGATTTGCCAGGCAGTTGTGACAGCTTGTATTGCAGCAAAGTTTGGAGTGGATGGGAACCCTGGAGAACTGCCAAAGTGGTATGCAATTGTGGTGGTGATGTTCATCTGCACATATGTTGCAGGATTCGCGTGGTCGTGGGGTCCCCTCGGATGGCTAGTCCCCAGTGAAATCTTCCCGTTGGAAATCCGATCAGCCGCGCAGAGCGTCAACGTTTCGGTCAACATGATCTTCACCTTCATCGTGGCTCAAATCTTCTTGACAATGCTCTGCCACTTGAAGTTCGGGCTCTTCCTTTTCTTTGCGTTCTTTGTGTTTGTGATGTCAATCTTCGTCTACTACTTCTTGCCGGAGACGAAGGGGATTCCGATCGAAGAGATGGGACAAGTATGGAGGACACATTGGTACTGGAAGAGATTCGTCAGCGAGGAAGATGGAGGAGGTTATGAGATGGGCAAGGCAGCTCAAACTGTCAAAACTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

185

Amino Acids

20.91

Weight (kDa)

6.72

Isoelectric Point (pI)

39.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 1 - 154 1.9e-42 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 405
AccII CGCG 2 cut(s) 200, 269
AciI CCGC 1 cut(s) 267
AclI AACGTT 1 cut(s) 282
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 2 cut(s) 9, 349
AjnI CCWGG 2 cut(s) 78, 130
AluBI AGCT 3 cut(s) 62, 95, 533
AluI AGCT 3 cut(s) 62, 95, 533
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 416
ApeKI GCWGC 2 cut(s) 104, 530
Asp700I GAANNNNTTC 1 cut(s) 485
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 1 cut(s) 212
AsuHPI GGTGA 2 cut(s) 178, 295
AvaII GGWCC 1 cut(s) 212
BanII GRGCYC 2 cut(s) 64, 360
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 2 cut(s) 116, 542
BccI CCATC 5 cut(s) 116, 216, 442, 497, 514
BciT130I CCWGG 2 cut(s) 80, 132
BcoDI GTCTC 1 cut(s) 416
BfaI CTAG 1 cut(s) 227
BisI GCNGC 3 cut(s) 105, 267, 531
BlsI GCNGC 3 cut(s) 106, 268, 532
Bme1390I CCNGG 2 cut(s) 80, 132
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 4 cut(s) 41, 128, 213, 214
BmrFI CCNGG 2 cut(s) 80, 132
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 71
BsaBI GATNNNNATC 3 cut(s) 173, 395, 437
BsaJI CCNNGG 2 cut(s) 130, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 2 cut(s) 234, 482
Bse8I GATNNNNATC 3 cut(s) 173, 395, 437
BseBI CCWGG 2 cut(s) 80, 132
BseDI CCNNGG 2 cut(s) 130, 217
BseGI GGATG 2 cut(s) 127, 227
BseJI GATNNNNATC 3 cut(s) 173, 395, 437
BseLI CCNNNNNNNGG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 234, 482
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 2 cut(s) 116, 542
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 2 cut(s) 200, 269
Bsh1285I CGRYCG 1 cut(s) 441
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 419
BslFI GGGAC 3 cut(s) 198, 215, 465
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 1 cut(s) 416
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 3 cut(s) 198, 215, 465
Bsp1286I GDGCHC 2 cut(s) 64, 360
Bsp143I GATC 3 cut(s) 260, 297, 438
BspACI CCGC 1 cut(s) 267
BspFNI CGCG 2 cut(s) 200, 269
BspLI GGNNCC 4 cut(s) 41, 128, 213, 214
BspQI GCTCTTC 1 cut(s) 365
BsrI ACTGG 2 cut(s) 234, 482
BssECI CCNNGG 2 cut(s) 130, 217
BssMI GATC 3 cut(s) 260, 297, 438
Bst2UI CCWGG 2 cut(s) 80, 132
Bst4CI ACNGT 2 cut(s) 541, 550
Bst6I CTCTTC 3 cut(s) 365, 438, 476
BstF5I GGATG 2 cut(s) 127, 227
BstFNI CGCG 2 cut(s) 200, 269
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 3 cut(s) 263, 300, 441
BstMAI GTCTC 1 cut(s) 416
BstMBI GATC 3 cut(s) 260, 297, 438
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 2 cut(s) 101, 197
BstNI CCWGG 2 cut(s) 80, 132
BstSCI CCNGG 2 cut(s) 78, 130
BstUI CGCG 2 cut(s) 200, 269
BstV1I GCAGC 2 cut(s) 116, 542
BtsCI GGATG 2 cut(s) 127, 227
BtsIMutI CAGTG 1 cut(s) 241
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 475
CspCI CAANNNNNGTGG 2 cut(s) 144, 179
CviAII CATG 1 cut(s) 295
CviJI RGCY 7 cut(s) 62, 95, 226, 266, 317, 358, 533
CviKI_1 RGCY 7 cut(s) 62, 95, 226, 266, 317, 358, 533
CviQI GTAC 1 cut(s) 475
DpnI GATC 3 cut(s) 262, 299, 440
DpnII GATC 3 cut(s) 260, 297, 438
Eam1104I CTCTTC 3 cut(s) 365, 438, 476
EarI CTCTTC 3 cut(s) 365, 438, 476
Ecl136II GAGCTC 1 cut(s) 62
Eco24I GRGCYC 2 cut(s) 64, 360
Eco47I GGWCC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 62
EcoICRI GAGCTC 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 212
EcoRII CCWGG 2 cut(s) 78, 130
EcoT38I GRGCYC 2 cut(s) 64, 360
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 1 cut(s) 298
FaiI YATR 7 cut(s) 15, 153, 184, 186, 296, 460, 516
FaqI GGGAC 3 cut(s) 198, 215, 465
FatI CATG 1 cut(s) 294
FauNDI CATATG 1 cut(s) 184
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 3 cut(s) 105, 267, 531
FokI GGATG 2 cut(s) 134, 234
FriOI GRGCYC 2 cut(s) 64, 360
Fsp4HI GCNGC 3 cut(s) 105, 267, 531
FspBI CTAG 1 cut(s) 227
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 105, 267, 531
GsuI CTGGAG 1 cut(s) 153
HapII CCGG 1 cut(s) 419
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 271
Hin1II CATG 1 cut(s) 298
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HincII GTYRAC 3 cut(s) 22, 280, 292
HindII GTYRAC 3 cut(s) 22, 280, 292
HinfI GANTC 3 cut(s) 195, 433, 486
HpaII CCGG 1 cut(s) 419
HphI GGTGA 2 cut(s) 178, 295
Hpy166II GTNNAC 4 cut(s) 22, 280, 292, 406
Hpy188I TCNGA 3 cut(s) 221, 260, 438
Hpy188III TCNNGA 1 cut(s) 328
Hpy8I GTNNAC 4 cut(s) 22, 280, 292, 406
HpyAV CCTTC 3 cut(s) 30, 316, 421
HpyCH4III ACNGT 2 cut(s) 541, 550
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 4 cut(s) 104, 155, 180, 191
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 197
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 1 cut(s) 298
HspAI GCGC 1 cut(s) 269
KflI GGGWCCC 1 cut(s) 212
Kzo9I GATC 3 cut(s) 260, 297, 438
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 8 cut(s) 65, 92, 117, 144, 177, 247, 432, 463
Lsp1109I GCAGC 2 cut(s) 116, 542
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 88
MalI GATC 3 cut(s) 262, 299, 440
MboI GATC 3 cut(s) 260, 297, 438
MboII GAAGA 9 cut(s) 45, 235, 292, 316, 352, 391, 455, 493, 512
MfeI CAATTG 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 64, 360
MluCI AATT 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 6 cut(s) 46, 227, 456, 490, 500, 503
MroXI GAANNNNTTC 1 cut(s) 485
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 80, 132
MunI CAATTG 1 cut(s) 156
MvaI CCWGG 2 cut(s) 80, 132
MvnI CGCG 2 cut(s) 200, 269
MwoI GCNNNNNNNGC 2 cut(s) 101, 197
NdeI CATATG 1 cut(s) 184
NdeII GATC 3 cut(s) 260, 297, 438
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 4 cut(s) 41, 128, 213, 214
NmuCI GTSAC 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 3 cut(s) 195, 433, 486
PkrI GCNGC 3 cut(s) 106, 268, 532
Ple19I CGATCG 1 cut(s) 441
PpuMI RGGWCCY 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 64
Psp1406I AACGTT 1 cut(s) 282
Psp5II RGGWCCY 1 cut(s) 212
Psp6I CCWGG 2 cut(s) 78, 130
PspGI CCWGG 2 cut(s) 78, 130
PspN4I GGNNCC 4 cut(s) 41, 128, 213, 214
PspPI GGNCC 1 cut(s) 212
PspPPI RGGWCCY 1 cut(s) 212
PvuI CGATCG 1 cut(s) 441
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
SacI GAGCTC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 365
SatI GCNGC 3 cut(s) 105, 267, 531
Sau3AI GATC 3 cut(s) 260, 297, 438
Sau96I GGNCC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 80, 132
SduI GDGCHC 2 cut(s) 64, 360
SetI ASST 7 cut(s) 41, 64, 97, 285, 308, 514, 535
SinI GGWCC 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 1 cut(s) 156
SsiI CCGC 1 cut(s) 267
SspMI CTAG 1 cut(s) 227
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 2 cut(s) 78, 130
TaaI ACNGT 2 cut(s) 541, 550
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 441
TaqII GACCGA 1 cut(s) 277
TasI AATT 1 cut(s) 156
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 3 cut(s) 195, 433, 486
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 1 cut(s) 88
TseI GCWGC 2 cut(s) 104, 530
Tsp45I GTSAC 1 cut(s) 88
TspDTI ATGAA 2 cut(s) 163, 298
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.