pycom10g25660
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0015711
Physical Location & Seq
Forward (+)
27213974 .. 27214722
749 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g25660.1

Sequence Viewer

Length: 555 bp
ATGGTCTCAATCTATGGTGTTGACAAGTGGGGAAGAAGGTTCCTTTTCCTTGAGGGTGGAGCTCAAATGTTATTTTGCCAGGCGGTTGTGGCAGCTTGCATTGGTGCAAAGTTTGGAGTAAATGGGACCCCTGGAGAATTGCCAAAGTGGTATGCAATTGTGGTGGTGGTGTTCATCTGCGCATATGTTGCAGGATTCGCATGGTCATGGGGTCCCCTCGGATGGCTAGTCCCGAGTGAAATCTTCCCGTTGGAAATCCGATCAGCCGCACAGAGTGTCAACGTTTCAGTCAACATGATCTTCACCTTCATTGTGGCTCAAATCTTCCTGACAATGCTCTGCCACTTGAAGTTTGGGCTCTTCATTTTCTTTGCGTTCTTTGTGTTTGTGATGTCAATCTTCGTTTACTACTTCTTGCCTGAGACGAAGGGGATTCCGATCGAAGAGATGGGCCAAGTGTGGAGGTCGCATTGGTACTGGAAGAGATTCGTCAGTGCAGAAGAGGGAGGAGGTTATGAGATGGAGAAGGCATCTCAAACTGTCAAAAATGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

185

Amino Acids

20.87

Weight (kDa)

7.64

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sugar_tr PF00083 1 - 154 5.5e-42 Sugar (and other) transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 181
AciI CCGC 2 cut(s) 83, 267
AclI AACGTT 1 cut(s) 282
AdeI CACNNNGTG 1 cut(s) 275
AfaI GTAC 1 cut(s) 476
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 1 cut(s) 349
AjnI CCWGG 2 cut(s) 78, 130
AluBI AGCT 2 cut(s) 62, 95
AluI AGCT 2 cut(s) 62, 95
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 2 cut(s) 10, 416
Ama87I CYCGRG 1 cut(s) 232
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 1 cut(s) 92
Asp700I GAANNNNTTC 1 cut(s) 485
AspLEI GCGC 1 cut(s) 182
AspS9I GGNCC 3 cut(s) 126, 212, 451
AsuHPI GGTGA 1 cut(s) 295
AvaI CYCGRG 1 cut(s) 232
AvaII GGWCC 2 cut(s) 126, 212
BanII GRGCYC 2 cut(s) 64, 360
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 1 cut(s) 104
BccI CCATC 3 cut(s) 216, 442, 514
BciT130I CCWGG 2 cut(s) 80, 132
BcoDI GTCTC 2 cut(s) 10, 416
BfaI CTAG 1 cut(s) 227
BisI GCNGC 2 cut(s) 93, 267
BlsI GCNGC 2 cut(s) 94, 268
Bme1390I CCNGG 2 cut(s) 80, 132
Bme18I GGWCC 2 cut(s) 126, 212
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 3 cut(s) 126, 212, 451
BmiI GGNNCC 5 cut(s) 41, 127, 128, 213, 214
BmrFI CCNGG 2 cut(s) 80, 132
BmsI GCATC 1 cut(s) 539
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 71
BsaBI GATNNNNATC 2 cut(s) 395, 437
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 2 cut(s) 130, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse1I ACTGG 1 cut(s) 482
Bse8I GATNNNNATC 2 cut(s) 395, 437
BseBI CCWGG 2 cut(s) 80, 132
BseDI CCNNGG 2 cut(s) 130, 217
BseGI GGATG 1 cut(s) 227
BseJI GATNNNNATC 2 cut(s) 395, 437
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 411
BseNI ACTGG 1 cut(s) 482
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 1 cut(s) 104
BsgI GTGCAG 1 cut(s) 516
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 441
BsiHKAI GWGCWC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 232
BslFI GGGAC 3 cut(s) 139, 198, 215
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 2 cut(s) 10, 416
BsmBI CGTCTC 1 cut(s) 416
BsmFI GGGAC 3 cut(s) 139, 198, 215
BsnI GGCC 1 cut(s) 453
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 64, 360
Bsp143I GATC 3 cut(s) 260, 297, 438
BspACI CCGC 2 cut(s) 83, 267
BspANI GGCC 1 cut(s) 453
BspCNI CTCAG 1 cut(s) 412
BspLI GGNNCC 5 cut(s) 41, 127, 128, 213, 214
BspQI GCTCTTC 1 cut(s) 365
BspTNI GGTCTC 1 cut(s) 10
BsrI ACTGG 1 cut(s) 482
BssECI CCNNGG 2 cut(s) 130, 217
BssMI GATC 3 cut(s) 260, 297, 438
Bst2UI CCWGG 2 cut(s) 80, 132
Bst4CI ACNGT 1 cut(s) 541
Bst6I CTCTTC 4 cut(s) 365, 438, 476, 495
BstAPI GCANNNNNTGC 1 cut(s) 188
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 1 cut(s) 420
BstF5I GGATG 1 cut(s) 227
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 3 cut(s) 263, 300, 441
BstMAI GTCTC 2 cut(s) 10, 416
BstMBI GATC 3 cut(s) 260, 297, 438
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 3 cut(s) 89, 188, 197
BstNI CCWGG 2 cut(s) 80, 132
BstSCI CCNGG 2 cut(s) 78, 130
BstV1I GCAGC 1 cut(s) 104
BsuRI GGCC 1 cut(s) 453
BtsCI GGATG 1 cut(s) 227
BtsIMutI CAGTG 1 cut(s) 499
Cac8I GCNNGC 1 cut(s) 97
CfoI GCGC 1 cut(s) 182
Cfr13I GGNCC 3 cut(s) 126, 212, 451
Csp6I GTAC 1 cut(s) 475
CspCI CAANNNNNGTGG 2 cut(s) 144, 179
CviAII CATG 3 cut(s) 201, 207, 295
CviJI RGCY 7 cut(s) 62, 95, 226, 266, 317, 358, 453
CviKI_1 RGCY 7 cut(s) 62, 95, 226, 266, 317, 358, 453
CviQI GTAC 1 cut(s) 475
DdeI CTNAG 1 cut(s) 420
DpnI GATC 3 cut(s) 262, 299, 440
DpnII GATC 3 cut(s) 260, 297, 438
DraIII CACNNNGTG 1 cut(s) 275
Eam1104I CTCTTC 4 cut(s) 365, 438, 476, 495
EarI CTCTTC 4 cut(s) 365, 438, 476, 495
Ecl136II GAGCTC 1 cut(s) 62
Eco24I GRGCYC 2 cut(s) 64, 360
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 2 cut(s) 126, 212
Eco53kI GAGCTC 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 62
EcoO109I RGGNCCY 2 cut(s) 126, 212
EcoRII CCWGG 2 cut(s) 78, 130
EcoT38I GRGCYC 2 cut(s) 64, 360
Esp3I CGTCTC 1 cut(s) 416
FaeI CATG 3 cut(s) 204, 210, 298
FaiI YATR 8 cut(s) 15, 153, 184, 186, 202, 208, 296, 516
FaqI GGGAC 3 cut(s) 139, 198, 215
FatI CATG 3 cut(s) 200, 206, 294
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 2 cut(s) 93, 267
FokI GGATG 1 cut(s) 234
FriOI GRGCYC 2 cut(s) 64, 360
Fsp4HI GCNGC 2 cut(s) 93, 267
FspBI CTAG 1 cut(s) 227
FspI TGCGCA 1 cut(s) 181
GlaI GCGC 1 cut(s) 181
GluI GCNGC 2 cut(s) 93, 267
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 453
HhaI GCGC 1 cut(s) 182
Hin1II CATG 3 cut(s) 204, 210, 298
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 3 cut(s) 22, 280, 292
HindII GTYRAC 3 cut(s) 22, 280, 292
HinfI GANTC 3 cut(s) 195, 433, 486
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 4 cut(s) 22, 280, 292, 406
Hpy188I TCNGA 3 cut(s) 221, 260, 438
Hpy188III TCNNGA 2 cut(s) 232, 328
Hpy8I GTNNAC 4 cut(s) 22, 280, 292, 406
HpyAV CCTTC 4 cut(s) 30, 316, 421, 520
HpyCH4III ACNGT 1 cut(s) 541
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 5 cut(s) 99, 107, 155, 191, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 188, 197
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 3 cut(s) 204, 210, 298
HspAI GCGC 1 cut(s) 180
KflI GGGWCCC 2 cut(s) 126, 212
Kzo9I GATC 3 cut(s) 260, 297, 438
LguI GCTCTTC 1 cut(s) 365
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 8 cut(s) 65, 92, 117, 144, 177, 341, 432, 463
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 1 cut(s) 539
MaeI CTAG 1 cut(s) 227
MaeII ACGT 1 cut(s) 282
MalI GATC 3 cut(s) 262, 299, 440
MboI GATC 3 cut(s) 260, 297, 438
MboII GAAGA 9 cut(s) 45, 235, 292, 316, 352, 391, 455, 493, 512
MfeI CAATTG 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 64, 360
MluCI AATT 2 cut(s) 137, 156
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 6 cut(s) 46, 227, 456, 496, 500, 503
MroXI GAANNNNTTC 1 cut(s) 485
MslI CAYNNNNRTG 1 cut(s) 205
MspR9I CCNGG 2 cut(s) 80, 132
MunI CAATTG 1 cut(s) 156
MvaI CCWGG 2 cut(s) 80, 132
MwoI GCNNNNNNNGC 3 cut(s) 89, 188, 197
NdeI CATATG 1 cut(s) 184
NdeII GATC 3 cut(s) 260, 297, 438
NlaIII CATG 3 cut(s) 204, 210, 298
NlaIV GGNNCC 5 cut(s) 41, 127, 128, 213, 214
NsbI TGCGCA 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 365
PdmI GAANNNNTTC 1 cut(s) 485
PfeI GAWTC 3 cut(s) 195, 433, 486
PkrI GCNGC 2 cut(s) 94, 268
Ple19I CGATCG 1 cut(s) 441
PpuMI RGGWCCY 2 cut(s) 126, 212
Psp124BI GAGCTC 1 cut(s) 64
Psp1406I AACGTT 1 cut(s) 282
Psp5II RGGWCCY 2 cut(s) 126, 212
Psp6I CCWGG 2 cut(s) 78, 130
PspGI CCWGG 2 cut(s) 78, 130
PspN4I GGNNCC 5 cut(s) 41, 127, 128, 213, 214
PspPI GGNCC 3 cut(s) 126, 212, 451
PspPPI RGGWCCY 2 cut(s) 126, 212
PvuI CGATCG 1 cut(s) 441
RsaI GTAC 1 cut(s) 476
RsaNI GTAC 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 205
SacI GAGCTC 1 cut(s) 64
SapI GCTCTTC 1 cut(s) 365
SatI GCNGC 2 cut(s) 93, 267
Sau3AI GATC 3 cut(s) 260, 297, 438
Sau96I GGNCC 3 cut(s) 126, 212, 451
ScrFI CCNGG 2 cut(s) 80, 132
SduI GDGCHC 2 cut(s) 64, 360
SetI ASST 7 cut(s) 41, 64, 97, 285, 308, 467, 514
SfaNI GCATC 1 cut(s) 539
SinI GGWCC 2 cut(s) 126, 212
SmiMI CAYNNNNRTG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 2 cut(s) 137, 156
SsiI CCGC 2 cut(s) 83, 267
SspMI CTAG 1 cut(s) 227
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 2 cut(s) 78, 130
TaaI ACNGT 1 cut(s) 541
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 441
TasI AATT 2 cut(s) 137, 156
TauI GCSGC 1 cut(s) 269
TfiI GAWTC 3 cut(s) 195, 433, 486
TscAI CASTG 1 cut(s) 499
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 3 cut(s) 163, 298, 352
TspRI CASTG 1 cut(s) 499
VpaK11BI GGWCC 2 cut(s) 126, 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 485
XspI CTAG 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.