pycom06g03630

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009547
Physical Location & Seq
Forward (+)
4539478 .. 4543990
4513 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g03630.1

Sequence Viewer

Length: 495 bp
ATGGCAGCTGCTCCTTCGCCCTCGCCTGCACTAAGCAACTCCAGCTCTGGCGCCACTCGCCCAACCACCGCCGCCGCCGCCGACGCGCCTACAGTCGCACCCTTACACCCGGTGCACCGTACCGGCACGCCGCCCAAGACCCTTCGCGGCCTTAACAAGCCTAAGTGTAAGCAGTGCGGCAATGTCGCGCGCTCCAGGTGTCCATACGAGTCATGCAAGAGTTGCTGCTCAAAAGCTCAAAATCCATGTCATATTCATGTTTTGAAAACAAATGCAACTTTTCCAGACAAGGCACCAACTTCAAACTCTCCTCTATTTGACCATCAGTCAACCGAGGCATCACCATCAGGGAATTCCCATAGAGTTGCATCATTCCGGCAACTTTCGAACAATTTTGCTCAATTCAATAATCTGCATATTCCGCTTCGTTCAAAGAAGCCTTTGACCAGAAAGGTGATGCTGTGGCTATTAATGAATGGAGATTTTCCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

165

Amino Acids

17.58

Weight (kDa)

10.07

Isoelectric Point (pI)

62.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 55 - 91 1.8e-06 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 50, 292
AccII CGCG 4 cut(s) 86, 147, 188, 190
AciI CCGC 8 cut(s) 69, 72, 75, 78, 131, 147, 177, 422
AcsI RAATTY 1 cut(s) 352
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 1 cut(s) 121
AgsI TTSAA 4 cut(s) 265, 303, 406, 432
AhdI GACNNNNNGTC 1 cut(s) 325
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 3 cut(s) 8, 45, 236
AluI AGCT 3 cut(s) 8, 45, 236
Alw21I GWGCWC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 113
AoxI GGCC 1 cut(s) 148
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 3 cut(s) 5, 8, 225
ApoI RAATTY 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AseI ATTAAT 1 cut(s) 470
AspLEI GCGC 4 cut(s) 53, 88, 190, 192
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 333, 466
AsuII TTCGAA 1 cut(s) 386
BaeGI GKGCMC 1 cut(s) 117
BanI GGYRCC 2 cut(s) 50, 292
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 17, 212
BccI CCATC 2 cut(s) 330, 352
BciT130I CCWGG 1 cut(s) 196
BcnI CCSGG 1 cut(s) 110
BfmI CTRYAG 1 cut(s) 90
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 9 cut(s) 6, 9, 72, 75, 78, 131, 148, 178, 226
BlsI GCNGC 9 cut(s) 7, 10, 73, 76, 79, 132, 149, 179, 227
Bme1390I CCNGG 2 cut(s) 110, 196
BmeRI GACNNNNNGTC 1 cut(s) 325
BmiI GGNNCC 2 cut(s) 52, 294
BmrFI CCNGG 2 cut(s) 110, 196
BmsI GCATC 3 cut(s) 347, 377, 447
BpmI CTGGAG 2 cut(s) 25, 178
Bpu14I TTCGAA 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 333
Bse118I RCCGGY 1 cut(s) 122
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 333
BseMI GCAATG 1 cut(s) 187
BsePI GCGCGC 1 cut(s) 188
BseRI GAGGAG 1 cut(s) 300
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 2 cut(s) 17, 212
BsgI GTGCAG 1 cut(s) 12
Bsh1236I CGCG 4 cut(s) 86, 147, 188, 190
BshFI GGCC 1 cut(s) 150
BshNI GGYRCC 2 cut(s) 50, 292
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 3 cut(s) 110, 123, 376
BsnI GGCC 1 cut(s) 150
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 1 cut(s) 117
BspACI CCGC 8 cut(s) 69, 72, 75, 78, 131, 147, 177, 422
BspANI GGCC 1 cut(s) 150
BspFNI CGCG 4 cut(s) 86, 147, 188, 190
BspLI GGNNCC 2 cut(s) 52, 294
BspT104I TTCGAA 1 cut(s) 386
BspT107I GGYRCC 2 cut(s) 50, 292
BsrDI GCAATG 1 cut(s) 187
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 333
BssHII GCGCGC 1 cut(s) 188
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 2 cut(s) 94, 119
BstACI GRCGYC 1 cut(s) 51
BstAPI GCANNNNNTGC 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 27, 128, 190
BstDEI CTNAG 2 cut(s) 32, 162
BstFNI CGCG 4 cut(s) 86, 147, 188, 190
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 4 cut(s) 53, 88, 190, 192
BstMWI GCNNNNNNNGC 6 cut(s) 42, 57, 77, 83, 222, 421
BstNI CCWGG 1 cut(s) 196
BstSCI CCNGG 2 cut(s) 108, 194
BstSFI CTRYAG 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 4 cut(s) 86, 147, 188, 190
BstV1I GCAGC 2 cut(s) 17, 212
BsuRI GGCC 1 cut(s) 150
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 3 cut(s) 27, 128, 190
CfoI GCGC 4 cut(s) 53, 88, 190, 192
Cfr10I RCCGGY 1 cut(s) 122
CseI GACGC 1 cut(s) 92
Csp6I GTAC 1 cut(s) 120
CviAII CATG 3 cut(s) 213, 246, 257
CviJI RGCY 7 cut(s) 8, 45, 150, 160, 236, 439, 466
CviKI_1 RGCY 7 cut(s) 8, 45, 150, 160, 236, 439, 466
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 2 cut(s) 32, 162
DinI GGCGCC 1 cut(s) 52
DraIII CACNNNGTG 1 cut(s) 112
DriI GACNNNNNGTC 1 cut(s) 325
Eam1105I GACNNNNNGTC 1 cut(s) 325
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 194
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
FaeI CATG 3 cut(s) 216, 249, 260
FaiI YATR 7 cut(s) 205, 214, 247, 252, 258, 360, 417
FatI CATG 3 cut(s) 212, 245, 256
Fnu4HI GCNGC 9 cut(s) 6, 9, 72, 75, 78, 131, 148, 178, 226
Fsp4HI GCNGC 9 cut(s) 6, 9, 72, 75, 78, 131, 148, 178, 226
GlaI GCGC 4 cut(s) 52, 87, 189, 191
GluI GCNGC 9 cut(s) 6, 9, 72, 75, 78, 131, 148, 178, 226
GsuI CTGGAG 2 cut(s) 25, 178
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 150
HapII CCGG 3 cut(s) 110, 123, 376
HgaI GACGC 1 cut(s) 92
HhaI GCGC 4 cut(s) 53, 88, 190, 192
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 216, 249, 260
Hin6I GCGC 4 cut(s) 51, 86, 188, 190
HinP1I GCGC 4 cut(s) 51, 86, 188, 190
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 1 cut(s) 209
HpaII CCGG 3 cut(s) 110, 123, 376
HphI GGTGA 2 cut(s) 333, 466
Hpy166II GTNNAC 2 cut(s) 115, 330
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 2 cut(s) 115, 330
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 24, 152
HpyCH4III ACNGT 2 cut(s) 94, 119
HpyCH4V TGCA 6 cut(s) 29, 115, 216, 275, 368, 415
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 57, 77, 83, 222, 421
HpyF3I CTNAG 2 cut(s) 32, 162
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 216, 249, 260
HspAI GCGC 4 cut(s) 51, 86, 188, 190
KasI GGCGCC 1 cut(s) 50
LmnI GCTCC 2 cut(s) 16, 197
Lsp1109I GCAGC 2 cut(s) 17, 212
LweI GCATC 3 cut(s) 347, 377, 447
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 3 cut(s) 352, 391, 401
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 1 cut(s) 218
MnlI CCTC 3 cut(s) 31, 321, 328
MseI TTAA 3 cut(s) 153, 470, 493
MslI CAYNNNNRTG 1 cut(s) 255
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 3 cut(s) 110, 123, 376
MspR9I CCNGG 2 cut(s) 110, 196
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 4 cut(s) 86, 147, 188, 190
MwoI GCNNNNNNNGC 6 cut(s) 42, 57, 77, 83, 222, 421
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 1 cut(s) 110
NlaIII CATG 3 cut(s) 216, 249, 260
NlaIV GGNNCC 2 cut(s) 52, 294
NspV TTCGAA 1 cut(s) 386
PauI GCGCGC 1 cut(s) 188
PkrI GCNGC 9 cut(s) 7, 10, 73, 76, 79, 132, 149, 179, 227
PleI GAGTC 1 cut(s) 217
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 217
PshBI ATTAAT 1 cut(s) 470
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 2 cut(s) 52, 294
PteI GCGCGC 1 cut(s) 188
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
RseI CAYNNNNRTG 1 cut(s) 255
SaqAI TTAA 3 cut(s) 153, 470, 493
SatI GCNGC 9 cut(s) 6, 9, 72, 75, 78, 131, 148, 178, 226
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 2 cut(s) 110, 196
SduI GDGCHC 1 cut(s) 117
SetI ASST 5 cut(s) 10, 47, 200, 238, 456
SfaNI GCATC 3 cut(s) 347, 377, 447
SfcI CTRYAG 1 cut(s) 90
SfoI GGCGCC 1 cut(s) 52
SfuI TTCGAA 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 3 cut(s) 352, 391, 401
SsiI CCGC 8 cut(s) 69, 72, 75, 78, 131, 147, 177, 422
SspDI GGCGCC 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 108, 194
TaaI ACNGT 2 cut(s) 94, 119
TaqI TCGA 1 cut(s) 386
TasI AATT 3 cut(s) 352, 391, 401
TauI GCSGC 6 cut(s) 74, 77, 80, 133, 150, 180
Tru1I TTAA 3 cut(s) 153, 470, 493
Tru9I TTAA 3 cut(s) 153, 470, 493
TscAI CASTG 1 cut(s) 179
TseI GCWGC 3 cut(s) 5, 8, 225
TspDTI ATGAA 2 cut(s) 245, 488
TspRI CASTG 1 cut(s) 179
VneI GTGCAC 1 cut(s) 113
VspI ATTAAT 1 cut(s) 470
XapI RAATTY 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.