Rorug07G0100100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009547
Physical Location & Seq
Reverse (-)
7898086 .. 7901662
3577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0100100.1

Sequence Viewer

Length: 969 bp
ATGGACGGTGAGGAGTTGACTGAGCAGGAGACTGCTCTTTACGATCGCCAAATTAGGGTTTGGGGCGCTGATGCTCAGAGAAGACTGAGCAAAGCTCATATACTAGTCTGTGGAATTAATGGGACTACTGCCGAGTTTTGCAAGAACATTGTACTAGCAGGAGTTGGTAGTTTGACACTGGTCGATGATCGGGTAGCGACCGAAGAAGCCCTTTCTGCTAACTTTTTGATACCTTCTGATGAAAGTGTGTGTGGGGGGAAAACTCTAACCGAGCTTTGTCGCGATTCTCTGAAAGATTTCAATCCGATGGTTCGTGTTTCTGTGGAGAAAGGTGACTTGTCAAACTTTGGCGGTGAGTTCTATAGCAAGTTTGATGTTGTGGTTATCAGTTGTTGCCCTCTCTCTACAAAAAAGCTGATCAATGAAAAATGCCGAAAGTTATCAAAGCGGGTAGCATTTTACACAGTTGATTGTAGAGACTCTTGTGGTGAAATTTTTGTTGATCTGCAACTCCATAAATATTCGAAGCAAAAACTGGAGGAAACAATTGAATGTCAACACAATTATCCAAGTTTTGAGGAATCAATTTCAGTACCTTGGAAAGCACTTCCCAGGAAAATGTCAAAGTTGTACTTTGCTATGCGAGTGATAGAAAGGTTTGAAGATGCTGAGCAACGTAAACCTGGAGAAGTTTCAATTGCAGATCTTCCCGGTGTTCTGAAATTGAAGAATGAGTTCTGTGAAGCACAGTCGTTGAAGGAATCACATATTCCTGATGCCCTCCTAGAAAGATTGGTGACAGATCCAAGAGAATTTCCTCCAGTTTGTCCCATCATTGGGGGAATTCTTGGGCAGGAGGTTATCAAAGCAATATCAGGCAAAGGAGAGCCTCTCAAGAATTTCTTCTTCTTTGATGCTATGGATGGAAAGGGCATAATAGAGGACCTATCAAACAACACTGGAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

322

Amino Acids

35.95

Weight (kDa)

5.22

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 14 - 310 4.8e-24 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 282
AciI CCGC 2 cut(s) 351, 448
AclWI GGATC 1 cut(s) 797
AcsI RAATTY 4 cut(s) 492, 812, 843, 898
AfaI GTAC 3 cut(s) 153, 594, 632
AfiI CCNNNNNNNGG 3 cut(s) 55, 836, 837
AgsI TTSAA 6 cut(s) 301, 551, 662, 696, 727, 757
AhlI ACTAGT 1 cut(s) 103
AjnI CCWGG 2 cut(s) 611, 682
AluBI AGCT 4 cut(s) 95, 274, 415, 966
AluI AGCT 4 cut(s) 95, 274, 415, 966
Alw26I GTCTC 2 cut(s) 23, 471
AlwI GGATC 1 cut(s) 797
ApoI RAATTY 4 cut(s) 492, 812, 843, 898
AseI ATTAAT 1 cut(s) 117
Asp700I GAANNNNTTC 3 cut(s) 296, 734, 902
AspLEI GCGC 1 cut(s) 68
AspS9I GGNCC 1 cut(s) 943
AsuC2I CCSGG 1 cut(s) 711
AsuHPI GGTGA 5 cut(s) 20, 344, 365, 500, 808
AsuII TTCGAA 1 cut(s) 524
AvaII GGWCC 1 cut(s) 943
BbsI GAAGAC 1 cut(s) 88
BccI CCATC 3 cut(s) 301, 839, 917
BciT130I CCWGG 2 cut(s) 613, 684
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 1 cut(s) 711
BcoDI GTCTC 2 cut(s) 23, 471
BcuI ACTAGT 1 cut(s) 103
BfaI CTAG 3 cut(s) 104, 155, 785
BfmI CTRYAG 1 cut(s) 361
BfoI RGCGCY 1 cut(s) 69
BglII AGATCT 1 cut(s) 703
BlpI GCTNAGC 1 cut(s) 669
Bme1390I CCNGG 3 cut(s) 613, 684, 711
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 1 cut(s) 943
BmrFI CCNGG 3 cut(s) 613, 684, 711
BmsI GCATC 4 cut(s) 61, 655, 766, 904
BoxI GACNNNNGTC 1 cut(s) 179
BpiI GAAGAC 1 cut(s) 88
BplI GAGNNNNNCTC 4 cut(s) 79, 111, 876, 908
BpmI CTGGAG 3 cut(s) 557, 705, 804
Bpu1102I GCTNAGC 1 cut(s) 669
Bpu14I TTCGAA 1 cut(s) 524
BpuEI CTTGAG 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 596, 611
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 836, 837
Bse1I ACTGG 4 cut(s) 183, 540, 821, 964
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 2 cut(s) 613, 684
BseDI CCNNGG 2 cut(s) 596, 611
BseGI GGATG 1 cut(s) 928
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 3 cut(s) 55, 836, 837
BseMII CTCAG 4 cut(s) 12, 77, 89, 660
BseNI ACTGG 4 cut(s) 183, 540, 821, 964
BseRI GAGGAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 2 cut(s) 46, 201
BsiEI CGRYCG 2 cut(s) 46, 201
BsiSI CCGG 1 cut(s) 711
BslFI GGGAC 2 cut(s) 136, 813
BslI CCNNNNNNNGG 3 cut(s) 55, 836, 837
BsmAI GTCTC 2 cut(s) 23, 471
BsmFI GGGAC 2 cut(s) 136, 813
Bsp119I TTCGAA 1 cut(s) 524
Bsp143I GATC 6 cut(s) 43, 187, 417, 502, 703, 802
Bsp1720I GCTNAGC 1 cut(s) 669
Bsp68I TCGCGA 1 cut(s) 282
BspACI CCGC 2 cut(s) 351, 448
BspCNI CTCAG 4 cut(s) 13, 78, 88, 661
BspFNI CGCG 1 cut(s) 282
BspPI GGATC 1 cut(s) 797
BspT104I TTCGAA 1 cut(s) 524
BsrI ACTGG 4 cut(s) 183, 540, 821, 964
BssECI CCNNGG 2 cut(s) 596, 611
BssMI GATC 6 cut(s) 43, 187, 417, 502, 703, 802
BssT1I CCWWGG 1 cut(s) 596
Bst2UI CCWGG 2 cut(s) 613, 684
Bst4CI ACNGT 3 cut(s) 8, 466, 750
BstBI TTCGAA 1 cut(s) 524
BstDEI CTNAG 4 cut(s) 21, 75, 86, 669
BstF5I GGATG 1 cut(s) 928
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 6 cut(s) 46, 190, 420, 505, 706, 805
BstMAI GTCTC 2 cut(s) 23, 471
BstMBI GATC 6 cut(s) 43, 187, 417, 502, 703, 802
BstMCI CGRYCG 2 cut(s) 46, 201
BstMWI GCNNNNNNNGC 1 cut(s) 215
BstNI CCWGG 2 cut(s) 613, 684
BstPAI GACNNNNGTC 1 cut(s) 179
BstSCI CCNGG 3 cut(s) 611, 682, 709
BstSFI CTRYAG 1 cut(s) 361
BstUI CGCG 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 703, 802
BstYI RGATCY 2 cut(s) 703, 802
BtsCI GGATG 1 cut(s) 928
BtsIMutI CAGTG 2 cut(s) 176, 957
BtuMI TCGCGA 1 cut(s) 282
CfoI GCGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 943
Csp6I GTAC 3 cut(s) 152, 593, 631
CviJI RGCY 6 cut(s) 95, 209, 274, 415, 889, 966
CviKI_1 RGCY 6 cut(s) 95, 209, 274, 415, 889, 966
CviQI GTAC 3 cut(s) 152, 593, 631
DdeI CTNAG 4 cut(s) 21, 75, 86, 669
DpnI GATC 6 cut(s) 45, 189, 419, 504, 705, 804
DpnII GATC 6 cut(s) 43, 187, 417, 502, 703, 802
Eco130I CCWWGG 1 cut(s) 596
Eco47I GGWCC 1 cut(s) 943
EcoO109I RGGNCCY 1 cut(s) 943
EcoRI GAATTC 1 cut(s) 843
EcoRII CCWGG 2 cut(s) 611, 682
EcoT14I CCWWGG 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 596
FaiI YATR 8 cut(s) 99, 101, 363, 516, 641, 768, 920, 935
FalI AAGNNNNNCTT 6 cut(s) 195, 227, 617, 649, 887, 919
FaqI GGGAC 2 cut(s) 136, 813
FauI CCCGC 1 cut(s) 441
FbaI TGATCA 1 cut(s) 417
FokI GGATG 1 cut(s) 935
FspBI CTAG 3 cut(s) 104, 155, 785
GlaI GCGC 1 cut(s) 67
GsuI CTGGAG 3 cut(s) 557, 705, 804
HaeII RGCGCY 1 cut(s) 69
HapII CCGG 1 cut(s) 711
HhaI GCGC 1 cut(s) 68
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 2 cut(s) 18, 557
HindII GTYRAC 2 cut(s) 18, 557
HinfI GANTC 4 cut(s) 284, 479, 581, 761
HpaII CCGG 1 cut(s) 711
HphI GGTGA 5 cut(s) 20, 344, 365, 500, 808
Hpy166II GTNNAC 3 cut(s) 18, 557, 680
Hpy188I TCNGA 5 cut(s) 78, 238, 291, 306, 720
Hpy188III TCNNGA 3 cut(s) 281, 773, 895
Hpy8I GTNNAC 3 cut(s) 18, 557, 680
HpyAV CCTTC 2 cut(s) 243, 751
HpyCH4III ACNGT 3 cut(s) 8, 466, 750
HpyCH4IV ACGT 1 cut(s) 676
HpyCH4V TGCA 3 cut(s) 141, 508, 701
HpyF10VI GCNNNNNNNGC 1 cut(s) 215
HpyF3I CTNAG 4 cut(s) 21, 75, 86, 669
HpySE526I ACGT 1 cut(s) 676
HspAI GCGC 1 cut(s) 66
Ksp22I TGATCA 1 cut(s) 417
Kzo9I GATC 6 cut(s) 43, 187, 417, 502, 703, 802
LweI GCATC 4 cut(s) 61, 655, 766, 904
MaeI CTAG 3 cut(s) 104, 155, 785
MaeII ACGT 1 cut(s) 676
MaeIII GTNAC 2 cut(s) 332, 796
MalI GATC 6 cut(s) 45, 189, 419, 504, 705, 804
MboI GATC 6 cut(s) 43, 187, 417, 502, 703, 802
MboII GAAGA 7 cut(s) 93, 215, 674, 698, 739, 895, 898
MfeI CAATTG 2 cut(s) 546, 696
MflI RGATCY 2 cut(s) 703, 802
MlyI GAGTC 1 cut(s) 473
MnlI CCTC 9 cut(s) 4, 408, 532, 571, 791, 828, 850, 900, 934
MroXI GAANNNNTTC 3 cut(s) 296, 734, 902
MseI TTAA 1 cut(s) 117
MspI CCGG 1 cut(s) 711
MspR9I CCNGG 3 cut(s) 613, 684, 711
MunI CAATTG 2 cut(s) 546, 696
MvaI CCWGG 2 cut(s) 613, 684
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 215
NciI CCSGG 1 cut(s) 711
NdeII GATC 6 cut(s) 43, 187, 417, 502, 703, 802
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 2 cut(s) 332, 796
NruI TCGCGA 1 cut(s) 282
NspV TTCGAA 1 cut(s) 524
PdmI GAANNNNTTC 3 cut(s) 296, 734, 902
PfeI GAWTC 3 cut(s) 284, 581, 761
Ple19I CGATCG 1 cut(s) 46
PleI GAGTC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 473
PpuMI RGGWCCY 1 cut(s) 943
PshAI GACNNNNGTC 1 cut(s) 179
PshBI ATTAAT 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 943
Psp6I CCWGG 2 cut(s) 611, 682
PspGI CCWGG 2 cut(s) 611, 682
PspPI GGNCC 1 cut(s) 943
PspPPI RGGWCCY 1 cut(s) 943
PsuI RGATCY 2 cut(s) 703, 802
PvuI CGATCG 1 cut(s) 46
RruI TCGCGA 1 cut(s) 282
RsaI GTAC 3 cut(s) 153, 594, 632
RsaNI GTAC 3 cut(s) 152, 593, 631
SaqAI TTAA 1 cut(s) 117
Sau3AI GATC 6 cut(s) 43, 187, 417, 502, 703, 802
Sau96I GGNCC 1 cut(s) 943
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 3 cut(s) 613, 684, 711
SfaNI GCATC 4 cut(s) 61, 655, 766, 904
SfcI CTRYAG 1 cut(s) 361
SfuI TTCGAA 1 cut(s) 524
SinI GGWCC 1 cut(s) 943
SmlI CTYRAG 1 cut(s) 893
SmoI CTYRAG 1 cut(s) 893
SpeI ACTAGT 1 cut(s) 103
SsiI CCGC 2 cut(s) 351, 448
SspI AATATT 1 cut(s) 521
SspMI CTAG 3 cut(s) 104, 155, 785
StyD4I CCNGG 3 cut(s) 611, 682, 709
StyI CCWWGG 1 cut(s) 596
TaaI ACNGT 3 cut(s) 8, 466, 750
TaiI ACGT 1 cut(s) 679
TaqI TCGA 2 cut(s) 183, 524
TaqII GACCGA 1 cut(s) 215
TatI WGTACW 2 cut(s) 151, 630
TfiI GAWTC 3 cut(s) 284, 581, 761
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 2 cut(s) 183, 964
TseFI GTSAC 2 cut(s) 332, 796
Tsp45I GTSAC 2 cut(s) 332, 796
TspDTI ATGAA 2 cut(s) 255, 438
TspRI CASTG 2 cut(s) 183, 964
VpaK11BI GGWCC 1 cut(s) 943
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 4 cut(s) 492, 812, 843, 898
XmnI GAANNNNTTC 3 cut(s) 296, 734, 902
XspI CTAG 3 cut(s) 104, 155, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.