pycom16g23690

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0009547
Physical Location & Seq
Reverse (-)
24857883 .. 24862283
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g23690.1

Sequence Viewer

Length: 720 bp
ATGGCAGCTGCTCCTTCGCCCTCGCCTGCCCTAAGCAATAACAGCTCCGGCGACACTCGCCCCACCACTACCGCCACCGCTGCCGCACCTCCCGTCGCTCCCTTCCATCCGGTGCACCGTACCGACACGCCTCCCAAGACCCTTCGCGGCCTTAACAAGCCCAAGTGTAAGCAGTGCGGCAATGTCGCTCGCTCCAGGTGTCCATACGAGTCATGCAAAAGTTGTTGCTCAAAAGCTCAAAATCCATGTCATATTCATGTTTTGAAAACAAATACAACTTTTCCAGACAAGGCACCAACCTCAAACTCTCCTCTATTTGACCATCAATCAACCGATGCATCACCATCAGGGAATTCCCATAGAGTTGCATCATTCCGGCAACTTTCGATCAATTTTGCTCAATTCAATAATCTGCATATTCCGCTTCGTTCGAAGAAGCCTTTGACCAGAAAGGATGCCGTGGCTATTAATGAGTGGAGATTTTCCAAGTTGAGGGAATACAAGGACGGAAACATTGAAGTGGAAAATGAAGCTTTTGATCGATACATGCAGAACGTCAGCCTATTAGAGCAAGTGTTTTCTGTGGAATCTATTGCAGAGGAGTCAATGAGGGATGGATCATCCATATCAAACCAAAATACTACTACTAATACTACAGAAGATAACACTGAGGCTATGGTGTCTGGACTGAAGTTGAAGTTGATCGATCAAATCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

240

Amino Acids

26.29

Weight (kDa)

8.84

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 55 - 91 3.1e-06 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 292
AccII CGCG 1 cut(s) 147
AciI CCGC 6 cut(s) 72, 78, 84, 147, 177, 422
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 1 cut(s) 352
AcuI CTGAAG 1 cut(s) 710
AfaI GTAC 1 cut(s) 121
AfiI CCNNNNNNNGG 1 cut(s) 492
AgsI TTSAA 4 cut(s) 265, 406, 518, 697
AjnI CCWGG 1 cut(s) 194
AluBI AGCT 4 cut(s) 8, 45, 236, 533
AluI AGCT 4 cut(s) 8, 45, 236, 533
Alw21I GWGCWC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 113
AlwI GGATC 1 cut(s) 625
AoxI GGCC 1 cut(s) 148
ApaLI GTGCAC 1 cut(s) 113
ApeKI GCWGC 3 cut(s) 5, 8, 80
ApoI RAATTY 1 cut(s) 352
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AseI ATTAAT 1 cut(s) 468
AsuHPI GGTGA 1 cut(s) 333
AsuII TTCGAA 1 cut(s) 431
BaeGI GKGCMC 1 cut(s) 117
BanI GGYRCC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 2 cut(s) 17, 67
BccI CCATC 4 cut(s) 114, 330, 352, 608
BceAI ACGGC 1 cut(s) 443
BciT130I CCWGG 1 cut(s) 196
BfmI CTRYAG 1 cut(s) 654
BisI GCNGC 6 cut(s) 6, 9, 81, 84, 148, 178
BlsI GCNGC 6 cut(s) 7, 10, 82, 85, 149, 179
Bme1390I CCNGG 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 1 cut(s) 196
BmsI GCATC 4 cut(s) 325, 347, 377, 445
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 32
Bpu14I TTCGAA 1 cut(s) 431
Bsa29I ATCGAT 2 cut(s) 541, 705
BsaJI CCNNGG 1 cut(s) 459
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse3DI GCAATG 1 cut(s) 187
BseBI CCWGG 1 cut(s) 196
BseCI ATCGAT 2 cut(s) 541, 705
BseDI CCNNGG 1 cut(s) 459
BseGI GGATG 4 cut(s) 106, 460, 619, 620
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMI GCAATG 1 cut(s) 187
BseMII CTCAG 1 cut(s) 660
BseRI GAGGAG 2 cut(s) 300, 614
BseSI GKGCMC 1 cut(s) 117
BseXI GCAGC 2 cut(s) 17, 67
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 1 cut(s) 150
BshNI GGYRCC 1 cut(s) 292
BshVI ATCGAT 2 cut(s) 541, 705
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 3 cut(s) 48, 110, 376
BslI CCNNNNNNNGG 1 cut(s) 492
BsnI GGCC 1 cut(s) 150
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 5 cut(s) 387, 538, 617, 702, 706
BspACI CCGC 6 cut(s) 72, 78, 84, 147, 177, 422
BspANI GGCC 1 cut(s) 150
BspCNI CTCAG 1 cut(s) 661
BspDI ATCGAT 2 cut(s) 541, 705
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 294
BspPI GGATC 1 cut(s) 625
BspT104I TTCGAA 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 292
BsrDI GCAATG 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 459
BssMI GATC 5 cut(s) 387, 538, 617, 702, 706
Bst2UI CCWGG 1 cut(s) 196
Bst4CI ACNGT 1 cut(s) 119
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 27, 190
BstDEI CTNAG 2 cut(s) 32, 669
BstDSI CCRYGG 1 cut(s) 459
BstF5I GGATG 4 cut(s) 106, 460, 619, 620
BstFNI CGCG 1 cut(s) 147
BstKTI GATC 5 cut(s) 390, 541, 620, 705, 709
BstMBI GATC 5 cut(s) 387, 538, 617, 702, 706
BstMWI GCNNNNNNNGC 4 cut(s) 42, 57, 80, 421
BstNI CCWGG 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 194
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 1 cut(s) 117
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 2 cut(s) 17, 67
Bsu15I ATCGAT 2 cut(s) 541, 705
BsuRI GGCC 1 cut(s) 150
BsuTUI ATCGAT 2 cut(s) 541, 705
BtgI CCRYGG 1 cut(s) 459
BtsCI GGATG 4 cut(s) 106, 460, 619, 620
BtsI GCAGTG 1 cut(s) 179
BtsIMutI CAGTG 2 cut(s) 179, 666
Cac8I GCNNGC 2 cut(s) 27, 190
ClaI ATCGAT 2 cut(s) 541, 705
Csp6I GTAC 1 cut(s) 120
CviAII CATG 4 cut(s) 213, 246, 257, 547
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 2 cut(s) 32, 669
DpnI GATC 5 cut(s) 389, 540, 619, 704, 708
DpnII GATC 5 cut(s) 387, 538, 617, 702, 706
Eco57I CTGAAG 1 cut(s) 710
EcoRI GAATTC 1 cut(s) 352
EcoRII CCWGG 1 cut(s) 194
EcoT22I ATGCAT 1 cut(s) 340
FaeI CATG 4 cut(s) 216, 249, 260, 550
FatI CATG 4 cut(s) 212, 245, 256, 546
Fnu4HI GCNGC 6 cut(s) 6, 9, 81, 84, 148, 178
FokI GGATG 4 cut(s) 93, 467, 607, 626
Fsp4HI GCNGC 6 cut(s) 6, 9, 81, 84, 148, 178
GluI GCNGC 6 cut(s) 6, 9, 81, 84, 148, 178
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 1 cut(s) 150
HapII CCGG 3 cut(s) 48, 110, 376
Hin1II CATG 4 cut(s) 216, 249, 260, 550
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 3 cut(s) 209, 587, 602
HpaII CCGG 3 cut(s) 48, 110, 376
HphI GGTGA 1 cut(s) 333
Hpy166II GTNNAC 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 284, 684
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 3 cut(s) 24, 112, 152
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 7 cut(s) 115, 216, 338, 368, 415, 550, 596
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 57, 80, 421
HpyF3I CTNAG 2 cut(s) 32, 669
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 4 cut(s) 216, 249, 260, 550
Kzo9I GATC 5 cut(s) 387, 538, 617, 702, 706
LmnI GCTCC 4 cut(s) 16, 50, 103, 197
Lsp1109I GCAGC 2 cut(s) 17, 67
LweI GCATC 4 cut(s) 325, 347, 377, 445
MaeII ACGT 1 cut(s) 555
MalI GATC 5 cut(s) 389, 540, 619, 704, 708
MboI GATC 5 cut(s) 387, 538, 617, 702, 706
MboII GAAGA 2 cut(s) 445, 671
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 3 cut(s) 352, 391, 401
MlyI GAGTC 2 cut(s) 218, 611
MnlI CCTC 9 cut(s) 31, 99, 141, 310, 321, 486, 592, 603, 664
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 153, 468
MslI CAYNNNNRTG 2 cut(s) 255, 518
MspA1I CMGCKG 2 cut(s) 8, 80
MspI CCGG 3 cut(s) 48, 110, 376
MspR9I CCNGG 1 cut(s) 196
MvaI CCWGG 1 cut(s) 196
MvnI CGCG 1 cut(s) 147
MwoI GCNNNNNNNGC 4 cut(s) 42, 57, 80, 421
NdeII GATC 5 cut(s) 387, 538, 617, 702, 706
NlaIII CATG 4 cut(s) 216, 249, 260, 550
NlaIV GGNNCC 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 550
NspV TTCGAA 1 cut(s) 431
PfeI GAWTC 1 cut(s) 587
PkrI GCNGC 6 cut(s) 7, 10, 82, 85, 149, 179
PleI GAGTC 2 cut(s) 217, 610
PpsI GAGTC 2 cut(s) 217, 610
PshBI ATTAAT 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 194
PspGI CCWGG 1 cut(s) 194
PspN4I GGNNCC 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
RseI CAYNNNNRTG 2 cut(s) 255, 518
SaqAI TTAA 2 cut(s) 153, 468
SatI GCNGC 6 cut(s) 6, 9, 81, 84, 148, 178
Sau3AI GATC 5 cut(s) 387, 538, 617, 702, 706
SchI GAGTC 2 cut(s) 218, 611
ScrFI CCNGG 1 cut(s) 196
SduI GDGCHC 1 cut(s) 117
SetI ASST 8 cut(s) 10, 47, 91, 200, 238, 302, 535, 558
SfaNI GCATC 4 cut(s) 325, 347, 377, 445
SfcI CTRYAG 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 431
SmiMI CAYNNNNRTG 2 cut(s) 255, 518
Sse9I AATT 3 cut(s) 352, 391, 401
SsiI CCGC 6 cut(s) 72, 78, 84, 147, 177, 422
StyD4I CCNGG 1 cut(s) 194
TaaI ACNGT 1 cut(s) 119
TaiI ACGT 1 cut(s) 558
TaqI TCGA 4 cut(s) 386, 431, 541, 705
TasI AATT 3 cut(s) 352, 391, 401
TauI GCSGC 3 cut(s) 86, 150, 180
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 2 cut(s) 153, 468
Tru9I TTAA 2 cut(s) 153, 468
TscAI CASTG 2 cut(s) 179, 673
TseI GCWGC 3 cut(s) 5, 8, 80
TspDTI ATGAA 2 cut(s) 245, 543
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 2 cut(s) 179, 673
VneI GTGCAC 1 cut(s) 113
VspI ATTAAT 1 cut(s) 468
XapI RAATTY 1 cut(s) 352
XceI RCATGY 1 cut(s) 550
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.