pycom06g07000

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012611
Physical Location & Seq
Reverse (-)
11347713 .. 11349110
1398 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
ATGGACTCAAAGAAGCACGCCGACCTTGAATTCGCTTATGGGTCTGGTCACATAAACCCTCTTAAAGCAGTAAATCCAGGTCTCATCTTCGATACATCCGAAGCAGACTACATAAATTTCCTCTGCACGCAGGGTTACAATAGCACCACATTGAAAATCCTAACCGGTGACAACAACAGCAGTTGTGGCAGCACAAAGCCAGGCAAAGCATGGAACCTAAACTACCCCTCATTCTCCCTTTCACTCATCGAAGGTCAAGAGATCAATGCTGAGTTCCCAAGAACAGTCACCAATGTTGGCTCACCAAACTCAACTTGTACCATAAGCTTCTACACACCTGATGACATGATAACCGTTTCAGTTTCACCAGCTACCCTTTCTTTCTCTTCTGTTGGGGAGAAGAAATCGTTTACAGTGAAGGTAACTGGCCCAAAGGTTTCAAACCAGCCAATCAAATCTGGTTCAGTTGTATTGAGTGATGGGGTTCATGCGGTGAGGGCTCCACTGATACTGCTAAGAATACTAAAAGGAATTTACGTACACAATCAACTTGATAAACTCATCACAAGATATCAACATGGAATTTTTCTACGTAACCGGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

202

Amino Acids

21.89

Weight (kDa)

8.94

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 72 - 170 8.1e-24 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 491
AcsI RAATTY 4 cut(s) 29, 115, 531, 582
AfaI GTAC 2 cut(s) 319, 540
AgeI ACCGGT 1 cut(s) 164
AgsI TTSAA 3 cut(s) 29, 154, 441
AjnI CCWGG 2 cut(s) 76, 199
AluBI AGCT 2 cut(s) 327, 371
AluI AGCT 2 cut(s) 327, 371
Alw26I GTCTC 1 cut(s) 86
AoxI GGCC 1 cut(s) 427
ApeKI GCWGC 1 cut(s) 189
ApoI RAATTY 4 cut(s) 29, 115, 531, 582
ArsI GACNNNNNNTTYG 2 cut(s) 14, 46
AsiGI ACCGGT 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 428
AsuHPI GGTGA 5 cut(s) 179, 280, 294, 357, 505
BanII GRGCYC 1 cut(s) 502
BbvI GCAGC 1 cut(s) 201
BccI CCATC 1 cut(s) 473
BciT130I CCWGG 2 cut(s) 78, 201
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 604
BisI GCNGC 1 cut(s) 190
BlsI GCNGC 1 cut(s) 191
Bme1390I CCNGG 2 cut(s) 78, 201
BmgT120I GGNCC 1 cut(s) 428
BmiI GGNNCC 2 cut(s) 215, 501
BmrFI CCNGG 2 cut(s) 78, 201
BsaAI YACGTR 2 cut(s) 538, 593
BsaI GGTCTC 1 cut(s) 86
BsaWI WCCGGW 2 cut(s) 164, 597
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 1 cut(s) 430
BseBI CCWGG 2 cut(s) 78, 201
BseGI GGATG 1 cut(s) 95
BseMII CTCAG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 430
BseXI GCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 109
BshFI GGCC 1 cut(s) 429
BshTI ACCGGT 1 cut(s) 164
BsiSI CCGG 2 cut(s) 165, 598
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 1 cut(s) 429
Bso31I GGTCTC 1 cut(s) 86
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 1 cut(s) 491
BspANI GGCC 1 cut(s) 429
BspCNI CTCAG 1 cut(s) 262
BspLI GGNNCC 2 cut(s) 215, 501
BspTNI GGTCTC 1 cut(s) 86
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 1 cut(s) 430
BssAI RCCGGY 1 cut(s) 164
BssMI GATC 1 cut(s) 261
Bst2UI CCWGG 2 cut(s) 78, 201
Bst4CI ACNGT 3 cut(s) 286, 355, 415
Bst6I CTCTTC 1 cut(s) 391
BstBAI YACGTR 2 cut(s) 538, 593
BstC8I GCNNGC 2 cut(s) 18, 128
BstDEI CTNAG 2 cut(s) 270, 515
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 186, 497
BstNI CCWGG 2 cut(s) 78, 201
BstSCI CCNGG 2 cut(s) 76, 199
BstSNI TACGTA 2 cut(s) 538, 593
BstV1I GCAGC 1 cut(s) 201
BsuRI GGCC 1 cut(s) 429
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 2 cut(s) 420, 503
Cac8I GCNNGC 2 cut(s) 18, 128
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 318, 539
CspAI ACCGGT 1 cut(s) 164
CviAII CATG 4 cut(s) 210, 346, 488, 578
CviJI RGCY 7 cut(s) 199, 300, 327, 371, 429, 448, 500
CviKI_1 RGCY 7 cut(s) 199, 300, 327, 371, 429, 448, 500
CviQI GTAC 2 cut(s) 318, 539
DdeI CTNAG 2 cut(s) 270, 515
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 391
EarI CTCTTC 1 cut(s) 391
Eco105I TACGTA 2 cut(s) 538, 593
Eco24I GRGCYC 1 cut(s) 502
Eco31I GGTCTC 1 cut(s) 86
Eco32I GATATC 1 cut(s) 572
EcoRI GAATTC 1 cut(s) 29
EcoRII CCWGG 2 cut(s) 76, 199
EcoRV GATATC 1 cut(s) 572
EcoT38I GRGCYC 1 cut(s) 502
FaeI CATG 4 cut(s) 213, 349, 491, 581
FaiI YATR 8 cut(s) 39, 53, 113, 211, 323, 347, 489, 579
FatI CATG 4 cut(s) 209, 345, 487, 577
Fnu4HI GCNGC 1 cut(s) 190
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 190
FspBI CTAG 1 cut(s) 604
GluI GCNGC 1 cut(s) 190
HaeIII GGCC 1 cut(s) 429
HapII CCGG 2 cut(s) 165, 598
Hin1II CATG 4 cut(s) 213, 349, 491, 581
HindIII AAGCTT 1 cut(s) 325
HinfI GANTC 1 cut(s) 5
HpaII CCGG 2 cut(s) 165, 598
HphI GGTGA 5 cut(s) 179, 280, 294, 357, 505
Hpy166II GTNNAC 2 cut(s) 411, 541
Hpy188I TCNGA 1 cut(s) 100
Hpy188III TCNNGA 1 cut(s) 257
Hpy8I GTNNAC 2 cut(s) 411, 541
HpyAV CCTTC 2 cut(s) 245, 412
HpyCH4III ACNGT 3 cut(s) 286, 355, 415
HpyCH4IV ACGT 2 cut(s) 537, 592
HpyCH4V TGCA 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 497
HpyF3I CTNAG 2 cut(s) 270, 515
HpySE526I ACGT 2 cut(s) 537, 592
Hsp92II CATG 4 cut(s) 213, 349, 491, 581
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 1 cut(s) 201
MaeI CTAG 1 cut(s) 604
MaeII ACGT 2 cut(s) 537, 592
MaeIII GTNAC 6 cut(s) 47, 134, 167, 286, 421, 593
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 3 cut(s) 79, 378, 412
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 4 cut(s) 29, 115, 531, 582
MnlI CCTC 4 cut(s) 69, 131, 238, 489
MseI TTAA 1 cut(s) 63
MspI CCGG 2 cut(s) 165, 598
MspR9I CCNGG 2 cut(s) 78, 201
MvaI CCWGG 2 cut(s) 78, 201
MwoI GCNNNNNNNGC 2 cut(s) 186, 497
NdeII GATC 1 cut(s) 261
NlaIII CATG 4 cut(s) 213, 349, 491, 581
NlaIV GGNNCC 2 cut(s) 215, 501
NmuCI GTSAC 3 cut(s) 47, 167, 286
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PinAI ACCGGT 1 cut(s) 164
PkrI GCNGC 1 cut(s) 191
Ppu21I YACGTR 2 cut(s) 538, 593
Psp6I CCWGG 2 cut(s) 76, 199
PspGI CCWGG 2 cut(s) 76, 199
PspN4I GGNNCC 2 cut(s) 215, 501
PspPI GGNCC 1 cut(s) 428
PsrI GAACNNNNNNTAC 2 cut(s) 206, 238
RsaI GTAC 2 cut(s) 319, 540
RsaNI GTAC 2 cut(s) 318, 539
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 1 cut(s) 190
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 1 cut(s) 428
ScrFI CCNGG 2 cut(s) 78, 201
SduI GDGCHC 1 cut(s) 502
SnaBI TACGTA 2 cut(s) 538, 593
Sse9I AATT 4 cut(s) 29, 115, 531, 582
SsiI CCGC 1 cut(s) 491
SspMI CTAG 1 cut(s) 604
StyD4I CCNGG 2 cut(s) 76, 199
TaaI ACNGT 3 cut(s) 286, 355, 415
TaiI ACGT 2 cut(s) 540, 595
TaqI TCGA 2 cut(s) 90, 249
TasI AATT 4 cut(s) 29, 115, 531, 582
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 2 cut(s) 420, 510
TseFI GTSAC 3 cut(s) 47, 167, 286
TseI GCWGC 1 cut(s) 189
Tsp45I GTSAC 3 cut(s) 47, 167, 286
TspDTI ATGAA 1 cut(s) 476
TspRI CASTG 2 cut(s) 420, 510
XapI RAATTY 4 cut(s) 29, 115, 531, 582
XcmI CCANNNNNNNNNTGG 1 cut(s) 207
XspI CTAG 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.