pycom06g07030

Subtilase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012611
Physical Location & Seq
Reverse (-)
11355739 .. 11357291
1553 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 732 bp
ATGGAAGGCAAAGTCATCGGAGCCCGTTATTACAACAGTGAAGACGACTATGACAGCACCGAGTTCCATTCGCCAAGAGACTCCATGGGACATGGAAGCCACACTGCTTCGACTGCTGCAGGCCGTGACGTGCAAGCAAGTTACTTTGGACTAGCTTCCGGAACAGCAAGAGGTGGAGTCCCCAATGCTAGGATGGCTGTGTACAAAGTTTGCTGGATAGCAGATTGTGCGGCCGCAGATATCCTTGCAGCATTTGATGATGCAATAGCTGATGGAGTTGACATTATATCAACCTCAATTGGTTCAGATAAACCACTCCAGTACCTAAACGACCCCATTGCTATCGGGTCTTTCCACGCCATGAAAAAAGGTATATTGACCTCAAGTTCAGCTGGAAACAACGGGCCATACCCCACTACGGTTACTAATTATGCGCCTTGGATTCTGACTGTTGCCGCCAGCACCATCGACAGAAGATTTACCGCTAAAGCAGTTCTTGGGAATGGACAAGTTTACTCTGTGAGTTTGACACTACAATTAAAAGGTTTTTCTGATATGAATTATAACTTCAAAATCTTATATTATGCACAGGCCTTACGGGAAGGCTTTTATATATACATCACAACAACACTTTACAGATTAAACTATAAGTTGCAGTCTGCACACCCATACCGCAGCACTATCTATACACCTTTACATATTAAACTAACAGCACACACACACATGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 Peptidase_S8

Protein Analysis

244

Amino Acids

26.54

Weight (kDa)

7.78

Isoelectric Point (pI)

28.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 10 - 166 7.8e-19 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012611)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g01310
malus_domestica MD06G1070500.v1.1
prunus_persica Prupe.5G088500_v2.0.a1 Prupe.5G088600_v2.0.a1
pyrus_communis pycom06g07000 pycom06g07030
rosa_chinensis RchiOBHm_Chr7g0199611
rosa_laevigata RLG00000003781
rosa_multiflora Rmu_sc0009191.1_g000006
rosa_roxburghii Rroxscaffold_3G00256540
rosa_rugosa Rorug07G0055500
rosa_samantha Rh7AG180400 Rh7AG180600 Rh7BG183400 Rh7CG190100 Rh7CG190300 Rh7DG183300 Rh7DG183500
rosa_wichuraiana Rw7G015850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 564
AccIII TCCGGA 1 cut(s) 158
AciI CCGC 5 cut(s) 230, 234, 456, 483, 673
AcoI YGGCCR 1 cut(s) 231
AfaI GTAC 2 cut(s) 203, 323
AfiI CCNNNNNNNGG 2 cut(s) 189, 418
AgsI TTSAA 1 cut(s) 571
AjiI CACGTC 1 cut(s) 130
AluBI AGCT 3 cut(s) 155, 269, 392
AluI AGCT 3 cut(s) 155, 269, 392
Alw26I GTCTC 1 cut(s) 72
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 4 cut(s) 121, 231, 404, 591
ApeKI GCWGC 3 cut(s) 116, 248, 675
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 1 cut(s) 404
BaeI ACNNNNGTAYC 2 cut(s) 305, 338
BanII GRGCYC 1 cut(s) 25
BbsI GAAGAC 1 cut(s) 48
BbvI GCAGC 3 cut(s) 103, 260, 687
BccI CCATC 3 cut(s) 187, 266, 473
BceAI ACGGC 1 cut(s) 108
BcgI CGANNNNNNTGC 3 cut(s) 32, 320, 354
BcoDI GTCTC 1 cut(s) 72
BfaI CTAG 2 cut(s) 152, 189
BfmI CTRYAG 1 cut(s) 117
BisI GCNGC 6 cut(s) 117, 231, 234, 249, 456, 676
BlsI GCNGC 6 cut(s) 118, 232, 235, 250, 457, 677
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 404
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 1 cut(s) 250
BpiI GAAGAC 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 367
BsaJI CCNNGG 2 cut(s) 84, 437
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 418
Bse1I ACTGG 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 158
BseDI CCNNGG 2 cut(s) 84, 437
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 189, 418
BseMI GCAATG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 319
BseX3I CGGCCG 1 cut(s) 231
BseXI GCAGC 3 cut(s) 103, 260, 687
BsgI GTGCAG 1 cut(s) 645
Bsh1285I CGRYCG 1 cut(s) 234
BshFI GGCC 4 cut(s) 123, 233, 406, 593
BsiEI CGRYCG 1 cut(s) 234
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 2 cut(s) 102, 164
BslI CCNNNNNNNGG 2 cut(s) 189, 418
BsmAI GTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 102, 164
BsnI GGCC 4 cut(s) 123, 233, 406, 593
Bsp1286I GDGCHC 1 cut(s) 25
Bsp13I TCCGGA 1 cut(s) 158
Bsp1407I TGTACA 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 5 cut(s) 230, 234, 456, 483, 673
BspANI GGCC 4 cut(s) 123, 233, 406, 593
BspEI TCCGGA 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 336
BsrGI TGTACA 1 cut(s) 201
BsrI ACTGG 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 84, 437
BssT1I CCWWGG 2 cut(s) 84, 437
Bst4CI ACNGT 3 cut(s) 38, 421, 451
BstAPI GCANNNNNTGC 1 cut(s) 227
BstAUI TGTACA 1 cut(s) 201
BstC8I GCNNGC 3 cut(s) 121, 135, 460
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 198
BstHHI GCGC 1 cut(s) 436
BstMAI GTCTC 1 cut(s) 72
BstMCI CGRYCG 1 cut(s) 234
BstMWI GCNNNNNNNGC 3 cut(s) 113, 194, 227
BstNSI RCATGY 1 cut(s) 727
BstSFI CTRYAG 1 cut(s) 117
BstV1I GCAGC 3 cut(s) 103, 260, 687
BstV2I GAAGAC 1 cut(s) 48
BstZI CGGCCG 1 cut(s) 231
BsuRI GGCC 4 cut(s) 123, 233, 406, 593
BtgI CCRYGG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 43, 102
Cac8I GCNNGC 3 cut(s) 121, 135, 460
CciNI GCGGCCGC 1 cut(s) 231
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 1 cut(s) 404
Csp6I GTAC 2 cut(s) 202, 322
CviAII CATG 4 cut(s) 85, 92, 361, 724
CviQI GTAC 2 cut(s) 202, 322
EaeI YGGCCR 1 cut(s) 231
EagI CGGCCG 1 cut(s) 231
EclXI CGGCCG 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 84, 437
Eco147I AGGCCT 1 cut(s) 593
Eco24I GRGCYC 1 cut(s) 25
Eco32I GATATC 1 cut(s) 241
Eco52I CGGCCG 1 cut(s) 231
EcoRV GATATC 1 cut(s) 241
EcoT14I CCWWGG 2 cut(s) 84, 437
EcoT22I ATGCAT 1 cut(s) 729
EcoT38I GRGCYC 1 cut(s) 25
ErhI CCWWGG 2 cut(s) 84, 437
FaeI CATG 4 cut(s) 88, 95, 364, 727
FalI AAGNNNNNCTT 2 cut(s) 480, 512
FaqI GGGAC 2 cut(s) 102, 164
FatI CATG 4 cut(s) 84, 91, 360, 723
Fnu4HI GCNGC 6 cut(s) 117, 231, 234, 249, 456, 676
FokI GGATG 1 cut(s) 205
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 6 cut(s) 117, 231, 234, 249, 456, 676
FspBI CTAG 2 cut(s) 152, 189
GlaI GCGC 1 cut(s) 435
GluI GCNGC 6 cut(s) 117, 231, 234, 249, 456, 676
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 4 cut(s) 123, 233, 406, 593
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 436
Hin1II CATG 4 cut(s) 88, 95, 364, 727
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 3 cut(s) 80, 177, 442
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 3 cut(s) 202, 280, 514
Hpy188I TCNGA 4 cut(s) 20, 307, 447, 553
Hpy188III TCNNGA 1 cut(s) 159
Hpy8I GTNNAC 3 cut(s) 202, 280, 514
HpyAV CCTTC 1 cut(s) 596
HpyCH4III ACNGT 3 cut(s) 38, 421, 451
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 8 cut(s) 119, 133, 248, 263, 587, 655, 662, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 194, 227
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 4 cut(s) 88, 95, 364, 727
HspAI GCGC 1 cut(s) 434
Kpn2I TCCGGA 1 cut(s) 158
LmnI GCTCC 1 cut(s) 20
LpnPI CCDG 7 cut(s) 105, 172, 199, 332, 378, 472, 575
Lsp1109I GCAGC 3 cut(s) 103, 260, 687
LweI GCATC 1 cut(s) 250
MaeI CTAG 2 cut(s) 152, 189
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 3 cut(s) 125, 140, 421
MboII GAAGA 2 cut(s) 53, 486
MfeI CAATTG 1 cut(s) 297
MhlI GDGCHC 1 cut(s) 25
MluCI AATT 4 cut(s) 297, 427, 536, 559
MlyI GAGTC 2 cut(s) 74, 186
MnlI CCTC 3 cut(s) 164, 304, 391
Mph1103I ATGCAT 1 cut(s) 729
MroI TCCGGA 1 cut(s) 158
MseI TTAA 4 cut(s) 539, 641, 702, 730
MslI CAYNNNNRTG 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 297
MwoI GCNNNNNNNGC 3 cut(s) 113, 194, 227
NcoI CCATGG 1 cut(s) 84
NlaIII CATG 4 cut(s) 88, 95, 364, 727
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 125
NotI GCGGCCGC 1 cut(s) 231
NsiI ATGCAT 1 cut(s) 729
NspI RCATGY 1 cut(s) 727
PceI AGGCCT 1 cut(s) 593
PfeI GAWTC 1 cut(s) 442
PkrI GCNGC 6 cut(s) 118, 232, 235, 250, 457, 677
PleI GAGTC 2 cut(s) 74, 185
PpsI GAGTC 2 cut(s) 74, 185
PsiI TTATAA 1 cut(s) 564
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 404
PstI CTGCAG 1 cut(s) 121
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 203, 323
RsaNI GTAC 2 cut(s) 202, 322
RseI CAYNNNNRTG 1 cut(s) 722
SaqAI TTAA 4 cut(s) 539, 641, 702, 730
SatI GCNGC 6 cut(s) 117, 231, 234, 249, 456, 676
Sau96I GGNCC 1 cut(s) 404
SchI GAGTC 2 cut(s) 74, 186
SduI GDGCHC 1 cut(s) 25
SfaNI GCATC 1 cut(s) 250
SfcI CTRYAG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 722
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 4 cut(s) 297, 427, 536, 559
SseBI AGGCCT 1 cut(s) 593
SsiI CCGC 5 cut(s) 230, 234, 456, 483, 673
SspMI CTAG 2 cut(s) 152, 189
StuI AGGCCT 1 cut(s) 593
StyI CCWWGG 2 cut(s) 84, 437
TaaI ACNGT 3 cut(s) 38, 421, 451
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 110, 468
TasI AATT 4 cut(s) 297, 427, 536, 559
TatI WGTACW 1 cut(s) 201
TauI GCSGC 3 cut(s) 233, 236, 458
TfiI GAWTC 1 cut(s) 442
Tru1I TTAA 4 cut(s) 539, 641, 702, 730
Tru9I TTAA 4 cut(s) 539, 641, 702, 730
TscAI CASTG 2 cut(s) 43, 109
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 3 cut(s) 116, 248, 675
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 377, 572
TspRI CASTG 2 cut(s) 43, 109
XceI RCATGY 1 cut(s) 727
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
XspI CTAG 2 cut(s) 152, 189
Zsp2I ATGCAT 1 cut(s) 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.