pycom06g08050

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0006371
Physical Location & Seq
Forward (+)
13099243 .. 13101182
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGGTGAATAAATTATCCGACTGGTTGGAGAAGCTCATGCCTTTGTATATTATTTGGAATGCTTTTATCTGGGAAATAATTTGTTTCCTAAATTGCCCTTACAGTCAGTTTAAGGACTATTGCCTCAATTCATTTTATTATTTTTATTCTATCATGCTATTGATCAACAGTTCCAGTCTTCAAGACAAAACCCGGAACATAGCCCGCGGGAAAATGGAGGAAGACGGAGGAGAACCACCTCGACCGCCCCATCCGACGAGCTCCGAAGCCCTAACGTCAGACCCGCGCCTCGATCCACAGTCCACGTGTCCTGATTCCACACTCGATCCCAGTGAGGACAACCGCACCGATTCGAAACCCCCTCCGATGAACCAGACCGAGATTTTCCGCGCTCTCGAAGTGGTCGAGAGAGACTCCCTGGCCATAGCCGACAGCTTCACCTCCCTCTTCGACTCCCTCCGCCTGGCCCTCTCCGAGGTTACTAGCAACTCAGTTGATCACATGCATTGCTTTGGTGACGCTGCTGGCCGCCTTCAAGAATCTGTGCTTGATGCTGCTACCAAGGGAAATCGGTATATAAATTCATGTCTCAGATTAAATGAAGAAATGAAGGGCATTGACAGTCTAGCCTTGCAGCTAAAAATCTTGAGGAGAAATGTTGATGCTCTCGACTCAGGTGTGAACAAGCTCCTTCGTCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BORCS6

Protein Analysis

235

Amino Acids

26.5

Weight (kDa)

4.88

Isoelectric Point (pI)

61.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BORCS6 PF10157 130 - 224 9.1e-20 BLOC-1-related complex sub-unit 6 C-terminal helix
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 207, 286, 390
AciI CCGC 8 cut(s) 205, 207, 245, 284, 343, 388, 460, 529
AclWI GGATC 2 cut(s) 287, 320
AcoI YGGCCR 2 cut(s) 420, 526
AcsI RAATTY 1 cut(s) 580
AcvI CACGTG 1 cut(s) 306
AfiI CCNNNNNNNGG 2 cut(s) 463, 475
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 2 cut(s) 182, 536
AjnI CCWGG 2 cut(s) 417, 462
AluBI AGCT 5 cut(s) 34, 261, 435, 637, 688
AluI AGCT 5 cut(s) 34, 261, 435, 637, 688
Alw21I GWGCWC 1 cut(s) 263
Alw26I GTCTC 2 cut(s) 405, 593
AlwI GGATC 2 cut(s) 287, 320
AoxI GGCC 3 cut(s) 420, 465, 526
ApeKI GCWGC 3 cut(s) 521, 554, 634
ApoI RAATTY 1 cut(s) 580
AspLEI GCGC 2 cut(s) 288, 392
AspS9I GGNCC 1 cut(s) 466
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 3 cut(s) 16, 430, 527
AsuII TTCGAA 1 cut(s) 353
BalI TGGCCA 1 cut(s) 422
BanII GRGCYC 1 cut(s) 263
BbrPI CACGTG 1 cut(s) 306
BbsI GAAGAC 3 cut(s) 170, 228, 689
Bbv12I GWGCWC 1 cut(s) 263
BbvI GCAGC 3 cut(s) 508, 541, 646
BccI CCATC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 419, 464
BclI TGATCA 2 cut(s) 162, 496
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 405, 593
BfaI CTAG 2 cut(s) 483, 626
BisI GCNGC 4 cut(s) 522, 529, 555, 635
BlsI GCNGC 4 cut(s) 523, 530, 556, 636
Bme1390I CCNGG 3 cut(s) 193, 419, 464
BmgT120I GGNCC 1 cut(s) 466
BmrFI CCNGG 3 cut(s) 193, 419, 464
BmrI ACTGGG 1 cut(s) 324
BmsI GCATC 2 cut(s) 541, 652
BmuI ACTGGG 1 cut(s) 324
BpiI GAAGAC 3 cut(s) 170, 228, 689
BplI GAGNNNNNCTC 2 cut(s) 398, 430
Bpu14I TTCGAA 1 cut(s) 353
BpuEI CTTGAG 1 cut(s) 667
BpuMI CCSGG 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 205, 417, 474, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 463, 475
Bse1I ACTGG 3 cut(s) 26, 174, 330
Bse3DI GCAATG 1 cut(s) 505
BseBI CCWGG 2 cut(s) 419, 464
BseDI CCNNGG 4 cut(s) 205, 417, 474, 561
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 2 cut(s) 463, 475
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 3 cut(s) 504, 604, 687
BseNI ACTGG 3 cut(s) 26, 174, 330
BseRI GAGGAG 2 cut(s) 243, 664
BseXI GCAGC 3 cut(s) 508, 541, 646
Bsh1236I CGCG 3 cut(s) 207, 286, 390
Bsh1285I CGRYCG 1 cut(s) 245
BshFI GGCC 3 cut(s) 422, 467, 528
BsiEI CGRYCG 1 cut(s) 245
BsiHKAI GWGCWC 1 cut(s) 263
BsiSI CCGG 1 cut(s) 193
BslI CCNNNNNNNGG 2 cut(s) 463, 475
BsmAI GTCTC 2 cut(s) 405, 593
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 3 cut(s) 422, 467, 528
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 263
Bsp143I GATC 4 cut(s) 162, 292, 325, 496
BspACI CCGC 8 cut(s) 205, 207, 245, 284, 343, 388, 460, 529
BspANI GGCC 3 cut(s) 422, 467, 528
BspCNI CTCAG 3 cut(s) 503, 603, 686
BspFNI CGCG 3 cut(s) 207, 286, 390
BspPI GGATC 2 cut(s) 287, 320
BspT104I TTCGAA 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 505
BsrI ACTGG 3 cut(s) 26, 174, 330
BssECI CCNNGG 4 cut(s) 205, 417, 474, 561
BssMI GATC 4 cut(s) 162, 292, 325, 496
BssT1I CCWWGG 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 419, 464
Bst4CI ACNGT 4 cut(s) 104, 170, 300, 623
Bst6I CTCTTC 1 cut(s) 452
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 205, 526
BstDEI CTNAG 3 cut(s) 490, 590, 673
BstDSI CCRYGG 1 cut(s) 205
BstENI CCTNNNNNAGG 1 cut(s) 473
BstF5I GGATG 1 cut(s) 250
BstFNI CGCG 3 cut(s) 207, 286, 390
BstHHI GCGC 2 cut(s) 288, 392
BstKTI GATC 4 cut(s) 165, 295, 328, 499
BstMAI GTCTC 2 cut(s) 405, 593
BstMBI GATC 4 cut(s) 162, 292, 325, 496
BstMCI CGRYCG 1 cut(s) 245
BstNI CCWGG 2 cut(s) 419, 464
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 3 cut(s) 191, 417, 462
BstUI CGCG 3 cut(s) 207, 286, 390
BstV1I GCAGC 3 cut(s) 508, 541, 646
BstV2I GAAGAC 3 cut(s) 170, 228, 689
BsuRI GGCC 3 cut(s) 422, 467, 528
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 337
Cac8I GCNNGC 2 cut(s) 205, 526
CfoI GCGC 2 cut(s) 288, 392
Cfr13I GGNCC 1 cut(s) 466
Cfr42I CCGCGG 1 cut(s) 208
CseI GACGC 1 cut(s) 527
CviAII CATG 5 cut(s) 37, 154, 502, 585, 702
DdeI CTNAG 3 cut(s) 490, 590, 673
DpnI GATC 4 cut(s) 164, 294, 327, 498
DpnII GATC 4 cut(s) 162, 292, 325, 496
EaeI YGGCCR 2 cut(s) 420, 526
Eam1104I CTCTTC 1 cut(s) 452
EarI CTCTTC 1 cut(s) 452
EciI GGCGGA 1 cut(s) 449
Ecl136II GAGCTC 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 263
Eco53kI GAGCTC 1 cut(s) 261
Eco72I CACGTG 1 cut(s) 306
EcoICRI GAGCTC 1 cut(s) 261
EcoNI CCTNNNNNAGG 1 cut(s) 473
EcoRII CCWGG 2 cut(s) 417, 462
EcoT14I CCWWGG 1 cut(s) 561
EcoT22I ATGCAT 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 263
ErhI CCWWGG 1 cut(s) 561
FaeI CATG 5 cut(s) 40, 157, 505, 588, 705
FatI CATG 5 cut(s) 36, 153, 501, 584, 701
FauI CCCGC 3 cut(s) 200, 212, 291
FbaI TGATCA 2 cut(s) 162, 496
Fnu4HI GCNGC 4 cut(s) 522, 529, 555, 635
FokI GGATG 1 cut(s) 237
FriOI GRGCYC 1 cut(s) 263
Fsp4HI GCNGC 4 cut(s) 522, 529, 555, 635
FspBI CTAG 2 cut(s) 483, 626
GlaI GCGC 2 cut(s) 287, 391
GluI GCNGC 4 cut(s) 522, 529, 555, 635
HaeIII GGCC 3 cut(s) 422, 467, 528
HapII CCGG 1 cut(s) 193
HgaI GACGC 1 cut(s) 527
HhaI GCGC 2 cut(s) 288, 392
Hin1II CATG 5 cut(s) 40, 157, 505, 588, 705
Hin6I GCGC 2 cut(s) 286, 390
HinP1I GCGC 2 cut(s) 286, 390
HinfI GANTC 6 cut(s) 314, 350, 413, 452, 539, 671
HpaII CCGG 1 cut(s) 193
HphI GGTGA 3 cut(s) 16, 430, 527
Hpy166II GTNNAC 2 cut(s) 303, 682
Hpy188I TCNGA 7 cut(s) 19, 255, 265, 280, 366, 475, 593
Hpy188III TCNNGA 7 cut(s) 182, 311, 395, 406, 536, 646, 668
Hpy8I GTNNAC 2 cut(s) 303, 682
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 3 cut(s) 542, 604, 701
HpyCH4III ACNGT 4 cut(s) 104, 170, 300, 623
HpyCH4IV ACGT 2 cut(s) 275, 305
HpyCH4V TGCA 2 cut(s) 505, 634
HpyF3I CTNAG 3 cut(s) 490, 590, 673
HpySE526I ACGT 2 cut(s) 275, 305
Hsp92II CATG 5 cut(s) 40, 157, 505, 588, 705
HspAI GCGC 2 cut(s) 286, 390
Ksp22I TGATCA 2 cut(s) 162, 496
KspI CCGCGG 1 cut(s) 208
Kzo9I GATC 4 cut(s) 162, 292, 325, 496
LmnI GCTCC 2 cut(s) 266, 693
Lsp1109I GCAGC 3 cut(s) 508, 541, 646
LweI GCATC 2 cut(s) 541, 652
MaeI CTAG 2 cut(s) 483, 626
MaeII ACGT 2 cut(s) 275, 305
MaeIII GTNAC 2 cut(s) 478, 515
MalI GATC 4 cut(s) 164, 294, 327, 498
MboI GATC 4 cut(s) 162, 292, 325, 496
MboII GAAGA 5 cut(s) 170, 233, 439, 614, 689
MhlI GDGCHC 1 cut(s) 263
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 5 cut(s) 11, 78, 91, 127, 580
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 3 cut(s) 407, 446, 665
MmeI TCCRAC 3 cut(s) 6, 42, 278
Mox20I TGGCCA 1 cut(s) 422
Mph1103I ATGCAT 1 cut(s) 507
MscI TGGCCA 1 cut(s) 422
MseI TTAA 2 cut(s) 111, 596
Msp20I TGGCCA 1 cut(s) 422
MspA1I CMGCKG 1 cut(s) 207
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 3 cut(s) 193, 419, 464
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 2 cut(s) 419, 464
MvnI CGCG 3 cut(s) 207, 286, 390
NciI CCSGG 1 cut(s) 193
NdeII GATC 4 cut(s) 162, 292, 325, 496
NlaIII CATG 5 cut(s) 40, 157, 505, 588, 705
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 1 cut(s) 507
NspI RCATGY 1 cut(s) 505
NspV TTCGAA 1 cut(s) 353
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 3 cut(s) 314, 350, 539
PkrI GCNGC 4 cut(s) 523, 530, 556, 636
PleI GAGTC 3 cut(s) 407, 446, 665
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
PpsI GAGTC 3 cut(s) 407, 446, 665
Ppu21I YACGTR 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 263
Psp6I CCWGG 2 cut(s) 417, 462
PspCI CACGTG 1 cut(s) 306
PspGI CCWGG 2 cut(s) 417, 462
PspPI GGNCC 1 cut(s) 466
SacI GAGCTC 1 cut(s) 263
SacII CCGCGG 1 cut(s) 208
SaqAI TTAA 2 cut(s) 111, 596
SatI GCNGC 4 cut(s) 522, 529, 555, 635
Sau3AI GATC 4 cut(s) 162, 292, 325, 496
Sau96I GGNCC 1 cut(s) 466
SchI GAGTC 3 cut(s) 407, 446, 665
ScrFI CCNGG 3 cut(s) 193, 419, 464
SduI GDGCHC 1 cut(s) 263
SfaNI GCATC 2 cut(s) 541, 652
Sfr303I CCGCGG 1 cut(s) 208
SfuI TTCGAA 1 cut(s) 353
SgrBI CCGCGG 1 cut(s) 208
SmlI CTYRAG 1 cut(s) 646
SmoI CTYRAG 1 cut(s) 646
Sse9I AATT 5 cut(s) 11, 78, 91, 127, 580
SsiI CCGC 8 cut(s) 205, 207, 245, 284, 343, 388, 460, 529
SspMI CTAG 2 cut(s) 483, 626
SstI GAGCTC 1 cut(s) 263
StyD4I CCNGG 3 cut(s) 191, 417, 462
StyI CCWWGG 1 cut(s) 561
TaaI ACNGT 4 cut(s) 104, 170, 300, 623
TaiI ACGT 2 cut(s) 278, 308
TaqI TCGA 8 cut(s) 241, 291, 324, 353, 396, 405, 450, 669
TaqII GACCGA 1 cut(s) 392
TasI AATT 5 cut(s) 11, 78, 91, 127, 580
TauI GCSGC 1 cut(s) 531
TfiI GAWTC 3 cut(s) 314, 350, 539
Tru1I TTAA 2 cut(s) 111, 596
Tru9I TTAA 2 cut(s) 111, 596
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 515
TseI GCWGC 3 cut(s) 521, 554, 634
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 5 cut(s) 120, 383, 573, 615, 623
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 1 cut(s) 337
XagI CCTNNNNNAGG 1 cut(s) 473
XapI RAATTY 1 cut(s) 580
XceI RCATGY 1 cut(s) 505
XspI CTAG 2 cut(s) 483, 626
Zsp2I ATGCAT 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.