pycom06g18000

Component of the replication protein A complex (RPA) required for DNA recombination, repair and replication. The activity of RPA is mediated by single-stranded DNA binding and protein interactions. Probably involved in repair of double-strand DNA breaks (DSBs) induced by genotoxic stresses

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009976
Physical Location & Seq
Forward (+)
21898172 .. 21901271
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1884 bp
ATGCGATTTCGACCATCTTGGCGAACAAGTCGCCGGACGAGTCCGTTGAGCTGCCGGAGATCGATGGTTCAGGTCTGCGATCTCACCCCCAGGGGCAAGTCTTTCATGTTTACTGCTACTGATGGAACTGCGAAGCTAAAGGGGATGCTCTCCTCGCGGCTGGGCCCCCAGGTCACCTCTGGGGAGATTCAGAATCTGGGTCTCATTCGCGTTTTGGGTTACTCTGTTAATTTCAATTCGAACTTGTCTGAAAACTATCTGCTTGTAACAAAATGTGAGGTGGTTTCGCCTGCGCTTGAGGCAGAGGTGAAAAATGAGGTTAAAAGGGAAGATGTTGGGATTATATTGAATCCCAAGCAAGAAATGGTTTCAGAATCTGCTGCCCAAATTGTGCATGAGCAGGCTGGGAAGGTCAAAAGGGAAGATGTTGGGGTTATATTGAAGCCCAAGCAAGAAATGGTTTCAAAATCTGCTGCCCAAATTGTGCATGAGCAGGCTGGGAATATGGCTCCGTCTGCGAGAATGGCTATGACACGAAGGGTTCACCCGCTTGTTTCCTTGAACCCTTACCAAGGAAATTGGACCATAAAGGTTCGTGTTACAAACAAGGGTACCATGCGTACATACAAGAATGCTAGAGGTGAAGGTTGCGTCTTCAATGTGGAGTTGACGGATCAAGATGGTACACAAATTCAAGCAACAATGTTCAATGAAGCTGCAAAGAAGTTCTTCGAGAAGTTTGAGTTAGGGAAGGTTTATTACATTTCAAAGGGATCTCTGAGGGTTGCTAACAAGCAGTTTAAGACGGTGCAAAATGATTACGAAATGACTTTGAATGAGAATTCTGAGGTGGAAGAAGCTAGCAATGAAGCAGCTTTTGTTCCTGAAATAAAATTCAATTTTGTGCCCATCGATATGTTGGGTCCCCATGTTAATGGAAAGGAGCTTGTGGATGTTATTGGGGTTGTTCAAAATGTGTCTCCTACAATGAGCATTAGAAGGAAGAGCAACAATGAGAGTATTCCGAAGCGTGATATCACCATTGCTGATGAAACAAAGAAGACAGTTGTGGTTTCTTTATGGGGTGAGCTTGCAACTGGTGTAGGCCAGGAGTTGCTTGACATTGCTGATCAATCTCCCATAGTCGCTATCAAGTCCCTCAAAGTTGGAGACTTCCAAGGTGTATCATTGTCAACATTGAGCAGGAGCACAATACTAGTTAATCCAGAGTTGCCTGAAGCACAGAAGTTGAGGTCCTGGTATGATTCCGAAGGCAAAGGATCTTCATTGGCCTCCATTGGCTCTGGTATGAACCCATCAGCCAAGAATGGAGGAAGGTCCATGTACTCTGACAGAGTCACCCTGTATCATATATCCGAAGACCCGTCCTTGGGTGAGGATAAGCCTGCGTTTTTCAGTGTTAAAGCATTCATAAGTTCGATCAGGCCTGATCAGACAATGTGGTACCGTGCTTGCAAAACTTGCAACAAGAAAGTGACTGAAGCTATTGGGTCTGGTTATTGGTGTGAAGGGTGCCAGAAAAATGATGAAGAATGCAGTCTAAGGTATATATTAGTTGCAAGAGTTGCTGATGTAAGTGGGGAAACGTATCTTTCTCTTTTCAATGATGAAGCTGAGAGGGTCCTCGGGTGCTCTGCAGATGAACTGGACAAATTGAAATCAAAGGATGGAGACGAGAATGAATTCCAACAGAAGCTGAAAGAAGCAACTTGGGTTCCTCATCTTTTTAGGGTCAGTGTTACTCTGAATGAGTACAATAACGAGAAGCGGAAGAGGATAACTGCCAGGGCAGTTGCTCCAGTTGATTTTGCTGCTGAATCTAGGTTTTTGCTTGAAGACATATCGAAGATGAAGGTGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000228 GO:0000278 GO:0000723 GO:0000724 GO:0000725 GO:0000781 GO:0000784 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0003697 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005662 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006268 GO:0006278 GO:0006279 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007004 GO:0007049 GO:0007063 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010224 GO:0010556 GO:0010557 GO:0010564 GO:0010604 GO:0010638 GO:0010639 GO:0010833 GO:0010948 GO:0016043 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022402 GO:0022414 GO:0030894 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032200 GO:0032204 GO:0032206 GO:0032210 GO:0032212 GO:0032392 GO:0032508 GO:0032879 GO:0032880 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0033260 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0035861 GO:0042162 GO:0042592 GO:0043047 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0044786 GO:0045786 GO:0045839 GO:0045875 GO:0045930 GO:0045935 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051054 GO:0051098 GO:0051101 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051173 GO:0051276 GO:0051321 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0060249 GO:0060255 GO:0060341 GO:0065007 GO:0065008 GO:0065009 GO:0070013 GO:0070034 GO:0071103 GO:0071704 GO:0071840 GO:0071897 GO:0071922 GO:0071923 GO:0080090 GO:0090304 GO:0090734 GO:0097159 GO:0098687 GO:0098847 GO:0106111 GO:0106112 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902969 GO:1903046 GO:1903047 GO:1903827 GO:1903828 GO:1904356 GO:1904358 GO:1905405 GO:1905412 GO:1905634 GO:2000112 GO:2000278 GO:2000573 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
REPA_OB_2 Rep-A_N Rep_fac-A_C tRNA_anti-codon

Protein Analysis

628

Amino Acids

69.81

Weight (kDa)

8.58

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep-A_N PF04057 23 - 96 6.3e-11 Replication factor-A protein 1, N-terminal domain
DUF223 PF02721 182 - 286 4.1e-10 Domain of unknown function DUF223
tRNA_anti-codon PF01336 194 - 279 2e-09 OB-fold nucleic acid binding domain
REPA_OB_2 PF16900 311 - 408 4.3e-24 Replication protein A OB domain
Rep_fac-A_C PF08646 471 - 617 2.9e-45 Replication factor-A C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 611, 1464
AccB1I GGYRCC 3 cut(s) 611, 1464, 1533
AccII CGCG 2 cut(s) 157, 210
AciI CCGC 3 cut(s) 157, 548, 1789
AclWI GGATC 3 cut(s) 681, 781, 1288
AcsI RAATTY 4 cut(s) 690, 841, 893, 1703
AcuI CTGAAG 2 cut(s) 1257, 1521
AfaI GTAC 6 cut(s) 613, 622, 685, 1346, 1466, 1775
AfiI CCNNNNNNNGG 3 cut(s) 572, 1390, 1391
AhlI ACTAGT 1 cut(s) 1216
AjnI CCWGG 5 cut(s) 89, 168, 1107, 1256, 1805
Alw21I GWGCWC 2 cut(s) 1211, 1655
Alw26I GTCTC 4 cut(s) 206, 984, 1164, 1686
AlwI GGATC 3 cut(s) 681, 781, 1288
AlwNI CAGNNNCTG 3 cut(s) 196, 377, 1717
Ama87I CYCGRG 1 cut(s) 1646
AoxI GGCC 4 cut(s) 163, 1105, 1290, 1445
ApaI GGGCCC 1 cut(s) 167
ApeKI GCWGC 6 cut(s) 51, 380, 473, 716, 872, 1832
ApoI RAATTY 4 cut(s) 690, 841, 893, 1703
Asp700I GAANNNNTTC 2 cut(s) 728, 1703
Asp718I GGTACC 2 cut(s) 611, 1464
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 7 cut(s) 163, 164, 582, 923, 1254, 1338, 1642
AsuHPI GGTGA 9 cut(s) 76, 166, 319, 536, 653, 1030, 1097, 1351, 1406
AsuII TTCGAA 1 cut(s) 239
AsuNHI GCTAGC 1 cut(s) 860
AvaI CYCGRG 1 cut(s) 1646
AvaII GGWCC 5 cut(s) 582, 923, 1254, 1338, 1642
BaeGI GKGCMC 2 cut(s) 167, 909
BaeI ACNNNNGTAYC 2 cut(s) 595, 628
BanI GGYRCC 3 cut(s) 611, 1464, 1533
BanII GRGCYC 1 cut(s) 167
BarI GAAGNNNNNNTAC 2 cut(s) 743, 775
BbsI GAAGAC 4 cut(s) 646, 1067, 1386, 1863
Bbv12I GWGCWC 2 cut(s) 1211, 1655
BbvI GCAGC 6 cut(s) 38, 367, 460, 703, 884, 1819
BccI CCATC 7 cut(s) 22, 58, 116, 674, 917, 1324, 1682
BciT130I CCWGG 5 cut(s) 91, 170, 1109, 1258, 1807
BclI TGATCA 2 cut(s) 1129, 1450
BcoDI GTCTC 4 cut(s) 206, 984, 1164, 1686
BcuI ACTAGT 1 cut(s) 1216
BfaI CTAG 4 cut(s) 636, 861, 1217, 1842
BfmI CTRYAG 1 cut(s) 1656
BisI GCNGC 7 cut(s) 52, 158, 381, 474, 717, 873, 1833
BlsI GCNGC 7 cut(s) 53, 159, 382, 475, 718, 874, 1834
Bme1390I CCNGG 5 cut(s) 91, 170, 1109, 1258, 1807
Bme18I GGWCC 5 cut(s) 582, 923, 1254, 1338, 1642
BmeT110I CYCGRG 1 cut(s) 1646
BmgT120I GGNCC 7 cut(s) 163, 164, 582, 923, 1254, 1338, 1642
BmrFI CCNGG 5 cut(s) 91, 170, 1109, 1258, 1807
BmsI GCATC 1 cut(s) 135
BmtI GCTAGC 1 cut(s) 864
BpiI GAAGAC 4 cut(s) 646, 1067, 1386, 1863
BpmI CTGGAG 1 cut(s) 1803
Bpu14I TTCGAA 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 317
Bsa29I ATCGAT 2 cut(s) 62, 912
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 8 cut(s) 89, 90, 168, 571, 1177, 1389, 1645, 1806
Bsc4I CCNNNNNNNGG 3 cut(s) 572, 1390, 1391
Bse1I ACTGG 3 cut(s) 1102, 1671, 1820
Bse3DI GCAATG 3 cut(s) 871, 1041, 1122
BseBI CCWGG 5 cut(s) 91, 170, 1109, 1258, 1807
BseCI ATCGAT 2 cut(s) 62, 912
BseDI CCNNGG 8 cut(s) 89, 90, 168, 571, 1177, 1389, 1645, 1806
BseGI GGATG 3 cut(s) 150, 958, 1693
BseLI CCNNNNNNNGG 3 cut(s) 572, 1390, 1391
BseMI GCAATG 3 cut(s) 871, 1041, 1122
BseMII CTCAG 3 cut(s) 770, 837, 1626
BseNI ACTGG 3 cut(s) 1102, 1671, 1820
BseRI GAGGAG 1 cut(s) 142
BseSI GKGCMC 2 cut(s) 167, 909
BseXI GCAGC 6 cut(s) 38, 367, 460, 703, 884, 1819
BseYI CCCAGC 3 cut(s) 160, 404, 497
Bsh1236I CGCG 2 cut(s) 157, 210
BshFI GGCC 4 cut(s) 165, 1107, 1292, 1447
BshNI GGYRCC 3 cut(s) 611, 1464, 1533
BshVI ATCGAT 2 cut(s) 62, 912
BsiHKAI GWGCWC 2 cut(s) 1211, 1655
BsiHKCI CYCGRG 1 cut(s) 1646
BsiSI CCGG 2 cut(s) 34, 55
BslFI GGGAC 2 cut(s) 909, 1141
BslI CCNNNNNNNGG 3 cut(s) 572, 1390, 1391
BsmAI GTCTC 4 cut(s) 206, 984, 1164, 1686
BsmBI CGTCTC 1 cut(s) 1686
BsmFI GGGAC 2 cut(s) 909, 1141
BsmI GAATGC 3 cut(s) 637, 1427, 1559
BsnI GGCC 4 cut(s) 165, 1107, 1292, 1447
Bso31I GGTCTC 1 cut(s) 206
BsoBI CYCGRG 1 cut(s) 1646
Bsp119I TTCGAA 1 cut(s) 239
Bsp120I GGGCCC 1 cut(s) 163
Bsp1286I GDGCHC 4 cut(s) 167, 909, 1211, 1655
Bsp143I GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
BspACI CCGC 3 cut(s) 157, 548, 1789
BspANI GGCC 4 cut(s) 165, 1107, 1292, 1447
BspCNI CTCAG 3 cut(s) 771, 838, 1627
BspDI ATCGAT 2 cut(s) 62, 912
BspFNI CGCG 2 cut(s) 157, 210
BspMAI CTGCAG 1 cut(s) 1660
BspOI GCTAGC 1 cut(s) 864
BspPI GGATC 3 cut(s) 681, 781, 1288
BspQI GCTCTTC 1 cut(s) 998
BspT104I TTCGAA 1 cut(s) 239
BspT107I GGYRCC 3 cut(s) 611, 1464, 1533
BspTNI GGTCTC 1 cut(s) 206
BsrDI GCAATG 3 cut(s) 871, 1041, 1122
BsrI ACTGG 3 cut(s) 1102, 1671, 1820
BssECI CCNNGG 8 cut(s) 89, 90, 168, 571, 1177, 1389, 1645, 1806
BssMI GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
BssT1I CCWWGG 3 cut(s) 571, 1177, 1389
Bst2UI CCWGG 5 cut(s) 91, 170, 1109, 1258, 1807
Bst4CI ACNGT 3 cut(s) 808, 1066, 1469
Bst6I CTCTTC 2 cut(s) 998, 1787
BstAPI GCANNNNNTGC 2 cut(s) 1482, 1586
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 7 cut(s) 291, 402, 495, 862, 1092, 1407, 1474
BstDEI CTNAG 4 cut(s) 779, 846, 1562, 1635
BstEII GGTNACC 1 cut(s) 172
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 3 cut(s) 150, 958, 1693
BstFNI CGCG 2 cut(s) 157, 210
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 8 cut(s) 62, 82, 676, 776, 1132, 1283, 1443, 1453
BstMAI GTCTC 4 cut(s) 206, 984, 1164, 1686
BstMBI GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
BstMWI GCNNNNNNNGC 6 cut(s) 154, 299, 515, 524, 1482, 1586
BstNI CCWGG 5 cut(s) 91, 170, 1109, 1258, 1807
BstPI GGTNACC 1 cut(s) 172
BstSCI CCNGG 5 cut(s) 89, 168, 1107, 1256, 1805
BstSFI CTRYAG 1 cut(s) 1656
BstSLI GKGCMC 2 cut(s) 167, 909
BstUI CGCG 2 cut(s) 157, 210
BstV1I GCAGC 6 cut(s) 38, 367, 460, 703, 884, 1819
BstV2I GAAGAC 4 cut(s) 646, 1067, 1386, 1863
BstX2I RGATCY 2 cut(s) 773, 1280
BstXI CCANNNNNNTGG 1 cut(s) 935
BstYI RGATCY 2 cut(s) 773, 1280
Bsu15I ATCGAT 2 cut(s) 62, 912
BsuRI GGCC 4 cut(s) 165, 1107, 1292, 1447
BsuTUI ATCGAT 2 cut(s) 62, 912
BtsCI GGATG 3 cut(s) 150, 958, 1693
BtsIMutI CAGTG 2 cut(s) 1423, 1762
Cac8I GCNNGC 7 cut(s) 291, 402, 495, 862, 1092, 1407, 1474
CaiI CAGNNNCTG 3 cut(s) 196, 377, 1717
CfoI GCGC 1 cut(s) 295
Cfr13I GGNCC 7 cut(s) 163, 164, 582, 923, 1254, 1338, 1642
ClaI ATCGAT 2 cut(s) 62, 912
CseI GACGC 1 cut(s) 640
Csp6I GTAC 6 cut(s) 612, 621, 684, 1345, 1465, 1774
CviAII CATG 6 cut(s) 106, 395, 488, 616, 929, 1342
CviQI GTAC 6 cut(s) 612, 621, 684, 1345, 1465, 1774
DdeI CTNAG 4 cut(s) 779, 846, 1562, 1635
DpnI GATC 8 cut(s) 61, 81, 675, 775, 1131, 1282, 1442, 1452
DpnII GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
Eam1104I CTCTTC 2 cut(s) 998, 1787
EarI CTCTTC 2 cut(s) 998, 1787
Eco130I CCWWGG 3 cut(s) 571, 1177, 1389
Eco147I AGGCCT 1 cut(s) 1447
Eco24I GRGCYC 1 cut(s) 167
Eco31I GGTCTC 1 cut(s) 206
Eco32I GATATC 1 cut(s) 1036
Eco47I GGWCC 5 cut(s) 582, 923, 1254, 1338, 1642
Eco57I CTGAAG 2 cut(s) 1257, 1521
Eco88I CYCGRG 1 cut(s) 1646
Eco91I GGTNACC 1 cut(s) 172
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoO109I RGGNCCY 4 cut(s) 164, 923, 1254, 1642
EcoO65I GGTNACC 1 cut(s) 172
EcoRI GAATTC 2 cut(s) 841, 1703
EcoRII CCWGG 5 cut(s) 89, 168, 1107, 1256, 1805
EcoRV GATATC 1 cut(s) 1036
EcoT14I CCWWGG 3 cut(s) 571, 1177, 1389
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 3 cut(s) 571, 1177, 1389
Esp3I CGTCTC 1 cut(s) 1686
FaeI CATG 6 cut(s) 109, 398, 491, 619, 932, 1345
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 2 cut(s) 909, 1141
FatI CATG 6 cut(s) 105, 394, 487, 615, 928, 1341
FauI CCCGC 1 cut(s) 555
FbaI TGATCA 2 cut(s) 1129, 1450
Fnu4HI GCNGC 7 cut(s) 52, 158, 381, 474, 717, 873, 1833
FokI GGATG 3 cut(s) 157, 965, 1700
FriOI GRGCYC 1 cut(s) 167
Fsp4HI GCNGC 7 cut(s) 52, 158, 381, 474, 717, 873, 1833
FspBI CTAG 4 cut(s) 636, 861, 1217, 1842
GlaI GCGC 1 cut(s) 294
GluI GCNGC 7 cut(s) 52, 158, 381, 474, 717, 873, 1833
GsaI CCCAGC 3 cut(s) 164, 408, 501
GsuI CTGGAG 1 cut(s) 1803
HaeIII GGCC 4 cut(s) 165, 1107, 1292, 1447
HapII CCGG 2 cut(s) 34, 55
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 295
Hin1II CATG 6 cut(s) 109, 398, 491, 619, 932, 1345
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HincII GTYRAC 2 cut(s) 669, 1194
HindII GTYRAC 2 cut(s) 669, 1194
HinfI GANTC 8 cut(s) 40, 187, 193, 349, 374, 1265, 1356, 1838
HpaII CCGG 2 cut(s) 34, 55
HphI GGTGA 9 cut(s) 76, 166, 319, 536, 653, 1030, 1097, 1351, 1406
Hpy166II GTNNAC 5 cut(s) 111, 544, 669, 686, 1194
Hpy188III TCNNGA 4 cut(s) 677, 733, 884, 1226
Hpy8I GTNNAC 5 cut(s) 111, 544, 669, 686, 1194
HpyAV CCTTC 9 cut(s) 403, 531, 638, 745, 993, 1265, 1329, 1523, 1867
HpyCH4III ACNGT 3 cut(s) 808, 1066, 1469
HpyCH4IV ACGT 1 cut(s) 1607
HpyF10VI GCNNNNNNNGC 6 cut(s) 154, 299, 515, 524, 1482, 1586
HpyF3I CTNAG 4 cut(s) 779, 846, 1562, 1635
HpySE526I ACGT 1 cut(s) 1607
Hsp92II CATG 6 cut(s) 109, 398, 491, 619, 932, 1345
HspAI GCGC 1 cut(s) 293
KflI GGGWCCC 1 cut(s) 923
KpnI GGTACC 2 cut(s) 615, 1468
Ksp22I TGATCA 2 cut(s) 1129, 1450
Kzo9I GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
LguI GCTCTTC 1 cut(s) 998
LmnI GCTCC 4 cut(s) 514, 943, 1206, 1822
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 1 cut(s) 135
MaeI CTAG 4 cut(s) 636, 861, 1217, 1842
MaeII ACGT 1 cut(s) 1607
MaeIII GTNAC 7 cut(s) 172, 218, 265, 598, 1357, 1495, 1759
MalI GATC 8 cut(s) 61, 81, 675, 775, 1131, 1282, 1442, 1452
MboI GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
MflI RGATCY 2 cut(s) 773, 1280
MhlI GDGCHC 4 cut(s) 167, 909, 1211, 1655
MlyI GAGTC 2 cut(s) 49, 1365
MmeI TCCRAC 2 cut(s) 1147, 1732
MroXI GAANNNNTTC 2 cut(s) 728, 1703
MseI TTAA 6 cut(s) 228, 321, 801, 933, 1221, 1422
MslI CAYNNNNRTG 2 cut(s) 914, 933
MspI CCGG 2 cut(s) 34, 55
MspR9I CCNGG 5 cut(s) 91, 170, 1109, 1258, 1807
Mva1269I GAATGC 3 cut(s) 637, 1427, 1559
MvaI CCWGG 5 cut(s) 91, 170, 1109, 1258, 1807
MvnI CGCG 2 cut(s) 157, 210
MwoI GCNNNNNNNGC 6 cut(s) 154, 299, 515, 524, 1482, 1586
NdeII GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
NheI GCTAGC 1 cut(s) 860
NlaIII CATG 6 cut(s) 109, 398, 491, 619, 932, 1345
NmuCI GTSAC 3 cut(s) 172, 1357, 1495
NspV TTCGAA 1 cut(s) 239
PasI CCCWGGG 1 cut(s) 90
PceI AGGCCT 1 cut(s) 1447
PciSI GCTCTTC 1 cut(s) 998
PctI GAATGC 3 cut(s) 637, 1427, 1559
PdmI GAANNNNTTC 2 cut(s) 728, 1703
PfeI GAWTC 6 cut(s) 187, 193, 349, 374, 1265, 1838
PflFI GACNNNGTC 1 cut(s) 1355
PkrI GCNGC 7 cut(s) 53, 159, 382, 475, 718, 874, 1834
PleI GAGTC 2 cut(s) 48, 1364
PpsI GAGTC 2 cut(s) 48, 1364
PpuMI RGGWCCY 3 cut(s) 923, 1254, 1642
Psp5II RGGWCCY 3 cut(s) 923, 1254, 1642
Psp6I CCWGG 5 cut(s) 89, 168, 1107, 1256, 1805
PspEI GGTNACC 1 cut(s) 172
PspFI CCCAGC 3 cut(s) 160, 404, 497
PspGI CCWGG 5 cut(s) 89, 168, 1107, 1256, 1805
PspOMI GGGCCC 1 cut(s) 163
PspPI GGNCC 7 cut(s) 163, 164, 582, 923, 1254, 1338, 1642
PspPPI RGGWCCY 3 cut(s) 923, 1254, 1642
PstI CTGCAG 1 cut(s) 1660
PstNI CAGNNNCTG 3 cut(s) 196, 377, 1717
PsuI RGATCY 2 cut(s) 773, 1280
PsyI GACNNNGTC 1 cut(s) 1355
RsaI GTAC 6 cut(s) 613, 622, 685, 1346, 1466, 1775
RsaNI GTAC 6 cut(s) 612, 621, 684, 1345, 1465, 1774
RseI CAYNNNNRTG 2 cut(s) 914, 933
SapI GCTCTTC 1 cut(s) 998
SaqAI TTAA 6 cut(s) 228, 321, 801, 933, 1221, 1422
SatI GCNGC 7 cut(s) 52, 158, 381, 474, 717, 873, 1833
Sau3AI GATC 8 cut(s) 59, 79, 673, 773, 1129, 1280, 1440, 1450
Sau96I GGNCC 7 cut(s) 163, 164, 582, 923, 1254, 1338, 1642
SchI GAGTC 2 cut(s) 49, 1365
ScrFI CCNGG 5 cut(s) 91, 170, 1109, 1258, 1807
SduI GDGCHC 4 cut(s) 167, 909, 1211, 1655
SfaNI GCATC 1 cut(s) 135
SfcI CTRYAG 1 cut(s) 1656
SfuI TTCGAA 1 cut(s) 239
SinI GGWCC 5 cut(s) 582, 923, 1254, 1338, 1642
SmiMI CAYNNNNRTG 2 cut(s) 914, 933
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SpeI ACTAGT 1 cut(s) 1216
SseBI AGGCCT 1 cut(s) 1447
SsiI CCGC 3 cut(s) 157, 548, 1789
SspMI CTAG 4 cut(s) 636, 861, 1217, 1842
StuI AGGCCT 1 cut(s) 1447
StyD4I CCNGG 5 cut(s) 89, 168, 1107, 1256, 1805
StyI CCWWGG 3 cut(s) 571, 1177, 1389
TaaI ACNGT 3 cut(s) 808, 1066, 1469
TaiI ACGT 1 cut(s) 1610
TaqI TCGA 7 cut(s) 10, 62, 239, 732, 912, 1439, 1865
TatI WGTACW 2 cut(s) 1344, 1773
TauI GCSGC 1 cut(s) 160
TfiI GAWTC 6 cut(s) 187, 193, 349, 374, 1265, 1838
Tru1I TTAA 6 cut(s) 228, 321, 801, 933, 1221, 1422
Tru9I TTAA 6 cut(s) 228, 321, 801, 933, 1221, 1422
TscAI CASTG 2 cut(s) 1423, 1762
TseFI GTSAC 3 cut(s) 172, 1357, 1495
TseI GCWGC 6 cut(s) 51, 380, 473, 716, 872, 1832
Tsp45I GTSAC 3 cut(s) 172, 1357, 1495
TspGWI ACGGA 3 cut(s) 33, 501, 686
TspRI CASTG 2 cut(s) 1423, 1762
Tth111I GACNNNGTC 1 cut(s) 1355
VpaK11BI GGWCC 5 cut(s) 582, 923, 1254, 1338, 1642
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 4 cut(s) 690, 841, 893, 1703
XcmI CCANNNNNNNNNTGG 4 cut(s) 176, 361, 454, 916
XmnI GAANNNNTTC 2 cut(s) 728, 1703
XspI CTAG 4 cut(s) 636, 861, 1217, 1842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.