pycom06g18450

WD repeat and HMG-box DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005318
Physical Location & Seq
Forward (+)
22221462 .. 22225959
4498 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2787 bp
ATGAAGATCCGAACGTTGAAGCTTCGAGAGGCCCACATGCCCACGAGCAGCGGCGGCGGCCCGTCGTACTGCTCGGTGTTGTGGGACCAGAAAGCCTACCACGTGGTGACGACCTCCTCCTCCGACCCCACAATCGCTATCCACGACTCTCTTCTGCTGTCCAGCCCTCCCAAGCTCCTCCGCCACCACCGCGATGGTGTCACGGCTCTCGCTCTCAGCCCCAACTCCACCTGCCTCGCCTCCGGCTCCGTCGACCATTCCGTCAAGCTCTACAAGTTTCCAGGTGGAGAATTTCAGACTAATATAACCCGATTCACCCTGCCAATACGCGCCCTGGCGTTTAACAAATCCGGCACCCTATTGGCAGCTGCTGGTGATGATGAAGGCGTTAAGCTTATTAACACAGTTGATGGTTCAATTTCAAGAGTTCTCAAAGGACACAAAGGACCTGTGACCGGCTTGGGTTTCGATCCCCTCAGCGAATACTTGGCCTCTATTGATTCCACTGGGACTGTTATATATTGGGAACTTTCATCTGGGACCACATTGCATACCCTTAAAGGGGTAGCTCCTAACTCGGGTTTCGATCACTCCATCATGAATGTACTTAGCTGGAGTCCTGATGGTGACAAGTTAGCCGTGCCGGGGTTGAGAAATGATGTGGTGGTTTATGATAGAGACACAGCTGAAAAGCTGTTTTCGTTGAGAGGTGACCACACGCAGCCTATTTGTTCCTTGGCTTGGTCGCCTAACGGAAAATACATGGCTACTTCTGGTTTGGATAAGCAGGTTCTCATCTGGAATGTCGATCGGAAGCAGGACATTGACAGGCAGAACTTTGATGAGAGAATATGTTGCTTGGCGTGGAAGCCAATTGGCAATGCACTGGCTGTTATTGATTGTATGGGCAAGTATGGTGTGTGGGATTCGGTGACTCCTTCATCAATGAAGTCTCCCACTGAAGATGTTCCCAATCTACAGTCTAAGAACAGCAATGGGCTACTTTTGTTTGACGAAGAGGAGGGTCAGGAGCTTAGCACTTCTGGCAGTTTAAGTGATGTTGGTGAAGATAGTATTGGGGAATCCAAGCCACCTAGCAGGAAGAGGCCGCACAAGCACACTGCATACGAGGATGATTTGGAAGAGGATGGTTTTGATGGCTTTAGGTTGATACCAAATGTTGAGTTGGAGTCCCGTAAAAAAGCACGGCGTAAACACAGTGAAAATGCTGATAATGAGAATGAGGGAGTAAGCAACAAAATGACATCAATTAGATCGAAAATGCAGGTGGCATTCCAGCCTGGTGCTACTCCTTTGCAGCCCAGAAAAAGGCGCTTTCTGTGCTATAACATGCTTGGCTCCATAACTACAATTGAACATGATGGGTTCTCCCATATTGAGATAGATTTTCATGATACAAGTCGAGGCCCAAGAGTTCCATCAATGAATGATCATTTTGGTTTTACAATGGCTTCACTTAATGAGAATGGAAGCGTTTTTGCAAATCCATGCAAGGGCGAAAAGCACATGAGTACTCTCATGTATCGCCCATTCAGTAGCTGGGCGAATAATAGTGAGAAACATGTTCTCTCCCTTGATGGACCTGTCGTGACCGCATCAGGCTTCAAGGATGAGCTTGCGATTGTCACTCATGCTTCTGATTGTCTCCCCTCAAATGAGCAGATGCTTGAATTTAGAGTATTCAACATAACTAAGGGGAGCGAGCCTCTCAGAGGACGGCTGCCACTGACTCCTGGTTCACATTTAACATGGTTTGGATTTAGTGAAGAAGGGCAATTAAGCTCCTATGATTCCAAGGGAGCGATGAGAGTTTTTACAAGCCAATATGGCGGCAGTTGGCTCCCACTCTTCAGTGCTAGCAAAGAGAAGAAGTCAAATGAAAACTATTGGGTGGTTGGGTTGAATGCAAGCAAATTGTTTTGTATAGTTTGCAAAAGTCCTGACTTATACCCCCAAGTGATGCCGAAGCCAGTCCTCACTCCGCTAAATTTTTCGTTCCCTCTAGCATCCTCTGATCTAGGTGCAGAAGCCCTTGAAAATGAGTTTATACTAAACAACATGCACCTTGTACAGATTCAAAAGCAAATAGAAGAAGTGGGCGCTGCTGGTTTGGACACCACTTCACTTGACGATGAGGCTTTCAATACAGAAGCAGCTCAAGATAGATGCATCTTAAAGCTTATTGCGTCCTGCTGCAACGGAGATAAGCTTGTGAGAGCGACTGAACTCGTGAAGCTTCTGTCCCTCGAAAAATCAGTAAGGGGTGCAATTAAGCTTGTCACTGTTCTGAAGCTCCCTAACTTGGCTGAAAGGTTCAACAGCATACTGGAGGAAAGGTTGCTCAACGAGAGTAAAAGAACCGCGGAAACCACTTTCCCTACCTCAAACTGCAACGCATCTTTCATCACGGATGACATTGCAGCCGGAAAGAAAATGATCTCTACCGAGCAAAGTAAGTTGTCAGGAAACGGCATTCCATTATCAACACCAAAACTGTCTGCTCCCTTGTTTGCCAAGAAAGCGAAAACGCAAGAACCTAAAGCTGGAACAGAGAAAACAGACGAAAAGCAAACTGCAACGGCGGATGATGATGTTGGGAAGGTAAAAACGACAGAGGTGAAAAACGCCACAGAGGTGAACAACAATGCAGGAGAGATGAAGAAGGTAGGGGAAGTGTCTGAGGTATCAAATGATCAAGAAAAAGTGAAGAAGGCAGAAGCACCACGACGAGCATCTAATCCATTCATGAAGAAATTAATTAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mcl1_mid WD40

Protein Analysis

929

Amino Acids

101.71

Weight (kDa)

6.38

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Gbeta PF25391 21 - 134 9.7e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 23 - 138 9.7e-14 THOC3 beta-propeller domain
WD40_WDHD1_1st PF24817 26 - 307 2e-71 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 32 - 138 2.1e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 34 - 187 6.8e-23 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 35 - 184 1.3e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 58 - 91 5.6e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 59 - 188 2.9e-18 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 62 - 273 4.1e-09 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 62 - 214 1.7e-10 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 64 - 299 3.7e-31 Prp19 WD40 domain
WDR55 PF24796 64 - 187 4.4e-08 WDR55
Beta-prop_WDR3_2nd PF25172 64 - 188 8.2e-09 WDR3 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 116 - 270 6.2e-12 WDR19 first beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 145 - 277 5.4e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_Aladin PF25460 190 - 277 3.7e-08 Aladin seven-bladed propeller
Beta-prop_IFT122_1st PF23381 199 - 313 1.2e-07 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 200 - 318 5.1e-15 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 200 - 276 2.1e-08 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 201 - 316 5.1e-11 WDR5 beta-propeller domain
ANAPC4_WD40 PF12894 203 - 290 5.6e-07 Anaphase-promoting complex subunit 4 WD40 domain
WD40_CDC20-Fz PF24807 203 - 296 2.4e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 211 - 300 1.9e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_Aladin PF25460 227 - 309 5.6e-07 Aladin seven-bladed propeller
WD40 PF00400 238 - 268 2e-07 WD domain, G-beta repeat
Mcl1_mid PF12341 431 - 525 1.7e-28 Minichromosome loss protein, Mcl1, middle region
Mcl1_mid PF12341 527 - 675 4.3e-39 Minichromosome loss protein, Mcl1, middle region
Ctf4_C PF20946 684 - 787 3e-12 DNA polymerase alpha-binding protein Ctf4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 239, 1276
AasI GACNNNNNNGTC 1 cut(s) 260
Acc36I ACCTGC 3 cut(s) 239, 778, 1276
AccB1I GGYRCC 1 cut(s) 353
AccI GTMKAC 1 cut(s) 252
AccII CGCG 3 cut(s) 192, 330, 2384
AclI AACGTT 1 cut(s) 14
AclWI GGATC 1 cut(s) 464
AcsI RAATTY 3 cut(s) 290, 1691, 2008
AcuI CTGAAG 3 cut(s) 981, 1855, 2330
AcvI CACGTG 1 cut(s) 103
AdeI CACNNNGTG 1 cut(s) 106
AfaI GTAC 4 cut(s) 68, 606, 1534, 2091
AflIII ACRYGT 1 cut(s) 1582
AjnI CCWGG 4 cut(s) 280, 333, 1300, 1753
AjuI GAANNNNNNNTTGG 2 cut(s) 1059, 1091
AleI CACNNNNGTG 1 cut(s) 2654
Alw26I GTCTC 3 cut(s) 672, 957, 1670
AlwI GGATC 1 cut(s) 464
AlwNI CAGNNNCTG 2 cut(s) 371, 1560
Ama87I CYCGRG 1 cut(s) 577
AoxI GGCC 5 cut(s) 30, 58, 489, 1106, 1426
ApoI RAATTY 3 cut(s) 290, 1691, 2008
AseI ATTAAT 1 cut(s) 2777
Asp700I GAANNNNTTC 1 cut(s) 966
AspLEI GCGC 3 cut(s) 332, 1335, 2123
AspS9I GGNCC 7 cut(s) 31, 59, 85, 446, 540, 1427, 1601
AsuC2I CCSGG 1 cut(s) 645
AsuHPI GGTGA 9 cut(s) 118, 307, 386, 638, 722, 943, 1076, 2650, 2668
AsuNHI GCTAGC 1 cut(s) 1877
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 4 cut(s) 85, 446, 540, 1601
BanI GGYRCC 1 cut(s) 353
BauI CACGAG 2 cut(s) 43, 2249
BbrPI CACGTG 1 cut(s) 103
BbvCI CCTCAGC 1 cut(s) 476
BccI CCATC 9 cut(s) 188, 404, 602, 617, 1142, 1151, 1376, 1447, 1592
BceAI ACGGC 6 cut(s) 219, 623, 1223, 1754, 2506, 2616
BciT130I CCWGG 4 cut(s) 282, 335, 1302, 1755
BclI TGATCA 2 cut(s) 1450, 2713
BcnI CCSGG 1 cut(s) 645
BcoDI GTCTC 3 cut(s) 672, 957, 1670
BfaI CTAG 4 cut(s) 1095, 1878, 2024, 2039
BfmI CTRYAG 1 cut(s) 977
BfoI RGCGCY 2 cut(s) 1336, 2124
BfuAI ACCTGC 3 cut(s) 239, 778, 1276
BglI GCCNNNNNGGC 1 cut(s) 1848
BlpI GCTNAGC 1 cut(s) 1034
BmcAI AGTACT 1 cut(s) 1534
Bme1390I CCNGG 5 cut(s) 282, 335, 645, 1302, 1755
Bme18I GGWCC 4 cut(s) 85, 446, 540, 1601
BmeT110I CYCGRG 1 cut(s) 577
BmgT120I GGNCC 7 cut(s) 31, 59, 85, 446, 540, 1427, 1601
BmiI GGNNCC 6 cut(s) 86, 247, 355, 541, 1360, 1862
BmrFI CCNGG 5 cut(s) 282, 335, 645, 1302, 1755
BmrI ACTGGG 1 cut(s) 516
BmsI GCATC 8 cut(s) 1625, 1674, 1971, 2036, 2177, 2199, 2426, 2762
BmtI GCTAGC 1 cut(s) 1881
BmuI ACTGGG 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 1711, 1743
BpmI CTGGAG 2 cut(s) 634, 2369
Bpu10I CCTNAGC 1 cut(s) 476
Bpu1102I GCTNAGC 1 cut(s) 1034
BpuEI CTTGAG 1 cut(s) 2163
BpuMI CCSGG 1 cut(s) 645
BsaAI YACGTR 1 cut(s) 103
BsaJI CCNNGG 5 cut(s) 333, 644, 735, 1815, 2382
BsaXI ACNNNNNCTCC 2 cut(s) 2342, 2372
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 4 cut(s) 511, 891, 1991, 2352
Bse3DI GCAATG 4 cut(s) 545, 886, 1000, 2436
BseBI CCWGG 4 cut(s) 282, 335, 1302, 1755
BseDI CCNNGG 5 cut(s) 333, 644, 735, 1815, 2382
BseGI GGATG 6 cut(s) 1138, 1153, 1636, 2027, 2437, 2611
BseMI GCAATG 4 cut(s) 545, 886, 1000, 2436
BseMII CTCAG 4 cut(s) 229, 490, 1744, 2691
BseNI ACTGG 4 cut(s) 511, 891, 1991, 2352
BseRI GAGGAG 4 cut(s) 106, 109, 167, 1034
BseYI CCCAGC 1 cut(s) 1560
BsgI GTGCAG 1 cut(s) 2064
Bsh1236I CGCG 3 cut(s) 192, 330, 2384
Bsh1285I CGRYCG 1 cut(s) 811
BshFI GGCC 5 cut(s) 32, 60, 491, 1108, 1428
BshNI GGYRCC 1 cut(s) 353
BsiEI CGRYCG 1 cut(s) 811
BsiHKCI CYCGRG 1 cut(s) 577
BsiSI CCGG 5 cut(s) 243, 351, 456, 644, 2445
BslFI GGGAC 5 cut(s) 98, 523, 553, 1177, 2248
BsmAI GTCTC 3 cut(s) 672, 957, 1670
BsmFI GGGAC 5 cut(s) 98, 523, 553, 1177, 2248
BsmI GAATGC 3 cut(s) 1292, 1930, 2493
BsnI GGCC 5 cut(s) 32, 60, 491, 1108, 1428
BsoBI CYCGRG 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 2089
Bsp143I GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
Bsp1720I GCTNAGC 1 cut(s) 1034
BspANI GGCC 5 cut(s) 32, 60, 491, 1108, 1428
BspCNI CTCAG 4 cut(s) 228, 489, 1743, 2692
BspFNI CGCG 3 cut(s) 192, 330, 2384
BspHI TCATGA 3 cut(s) 597, 1411, 2766
BspLI GGNNCC 6 cut(s) 86, 247, 355, 541, 1360, 1862
BspMI ACCTGC 3 cut(s) 239, 778, 1276
BspOI GCTAGC 1 cut(s) 1881
BspPI GGATC 1 cut(s) 464
BspT107I GGYRCC 1 cut(s) 353
BsrDI GCAATG 4 cut(s) 545, 886, 1000, 2436
BsrFI RCCGGY 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 2089
BsrI ACTGG 4 cut(s) 511, 891, 1991, 2352
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 5 cut(s) 333, 644, 735, 1815, 2382
BssMI GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
BssSI CACGAG 2 cut(s) 43, 2249
BssT1I CCWWGG 2 cut(s) 735, 1815
Bst2BI CACGAG 2 cut(s) 43, 2249
Bst2UI CCWGG 4 cut(s) 282, 335, 1302, 1755
Bst4CI ACNGT 6 cut(s) 406, 514, 981, 1220, 2305, 2517
Bst6I CTCTTC 5 cut(s) 156, 1011, 1097, 1137, 1874
BstAUI TGTACA 1 cut(s) 2089
BstBAI YACGTR 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 1638, 1724, 1879, 1930
BstDEI CTNAG 8 cut(s) 215, 476, 608, 984, 1034, 1713, 1730, 2700
BstDSI CCRYGG 1 cut(s) 2382
BstEII GGTNACC 1 cut(s) 710
BstENI CCTNNNNNAGG 1 cut(s) 1731
BstF5I GGATG 6 cut(s) 1138, 1153, 1636, 2027, 2437, 2611
BstFNI CGCG 3 cut(s) 192, 330, 2384
BstH2I RGCGCY 2 cut(s) 1336, 2124
BstHHI GCGC 3 cut(s) 332, 1335, 2123
BstKTI GATC 9 cut(s) 9, 472, 589, 811, 1277, 1453, 2038, 2460, 2716
BstMAI GTCTC 3 cut(s) 672, 957, 1670
BstMBI GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
BstMCI CGRYCG 1 cut(s) 811
BstMWI GCNNNNNNNGC 8 cut(s) 54, 57, 189, 1044, 1114, 1341, 1848, 2540
BstNI CCWGG 4 cut(s) 282, 335, 1302, 1755
BstNSI RCATGY 4 cut(s) 40, 1354, 1586, 2083
BstPI GGTNACC 1 cut(s) 710
BstSCI CCNGG 5 cut(s) 280, 333, 643, 1300, 1753
BstSFI CTRYAG 1 cut(s) 977
BstUI CGCG 3 cut(s) 192, 330, 2384
BstX2I RGATCY 1 cut(s) 6
BstXI CCANNNNNNTGG 1 cut(s) 194
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 5 cut(s) 32, 60, 491, 1108, 1428
BtgI CCRYGG 1 cut(s) 2382
BtgZI GCGATG 2 cut(s) 207, 1838
BtsCI GGATG 6 cut(s) 1138, 1153, 1636, 2027, 2437, 2611
BtsI GCAGTG 1 cut(s) 1119
BtsIMutI CAGTG 8 cut(s) 504, 884, 957, 1119, 1225, 1745, 1879, 2301
BveI ACCTGC 3 cut(s) 239, 778, 1276
Cac8I GCNNGC 4 cut(s) 1638, 1724, 1879, 1930
CaiI CAGNNNCTG 2 cut(s) 371, 1560
CciI TCATGA 3 cut(s) 597, 1411, 2766
CfoI GCGC 3 cut(s) 332, 1335, 2123
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 7 cut(s) 31, 59, 85, 446, 540, 1427, 1601
Cfr42I CCGCGG 1 cut(s) 2385
CseI GACGC 1 cut(s) 2196
Csp6I GTAC 4 cut(s) 67, 605, 1533, 2090
CspCI CAANNNNNGTGG 2 cut(s) 2128, 2163
CviQI GTAC 4 cut(s) 67, 605, 1533, 2090
DdeI CTNAG 8 cut(s) 215, 476, 608, 984, 1034, 1713, 1730, 2700
DpnI GATC 9 cut(s) 8, 471, 588, 810, 1276, 1452, 2037, 2459, 2715
DpnII GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
DraIII CACNNNGTG 1 cut(s) 106
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 5 cut(s) 156, 1011, 1097, 1137, 1874
EarI CTCTTC 5 cut(s) 156, 1011, 1097, 1137, 1874
EciI GGCGGA 2 cut(s) 170, 2618
Eco130I CCWWGG 2 cut(s) 735, 1815
Eco47I GGWCC 4 cut(s) 85, 446, 540, 1601
Eco57I CTGAAG 3 cut(s) 981, 1855, 2330
Eco72I CACGTG 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 577
Eco91I GGTNACC 1 cut(s) 710
EcoNI CCTNNNNNAGG 1 cut(s) 1731
EcoO109I RGGNCCY 1 cut(s) 446
EcoO65I GGTNACC 1 cut(s) 710
EcoRII CCWGG 4 cut(s) 280, 333, 1300, 1753
EcoT14I CCWWGG 2 cut(s) 735, 1815
EcoT22I ATGCAT 1 cut(s) 2192
ErhI CCWWGG 2 cut(s) 735, 1815
FaqI GGGAC 5 cut(s) 98, 523, 553, 1177, 2248
FbaI TGATCA 2 cut(s) 1450, 2713
FblI GTMKAC 1 cut(s) 252
FokI GGATG 6 cut(s) 1145, 1160, 1643, 2014, 2444, 2618
FspBI CTAG 4 cut(s) 1095, 1878, 2024, 2039
GlaI GCGC 3 cut(s) 331, 1334, 2122
GsaI CCCAGC 1 cut(s) 1564
GsuI CTGGAG 2 cut(s) 634, 2369
HaeII RGCGCY 2 cut(s) 1336, 2124
HaeIII GGCC 5 cut(s) 32, 60, 491, 1108, 1428
HapII CCGG 5 cut(s) 243, 351, 456, 644, 2445
HgaI GACGC 1 cut(s) 2196
HhaI GCGC 3 cut(s) 332, 1335, 2123
Hin6I GCGC 3 cut(s) 330, 1333, 2121
HinP1I GCGC 3 cut(s) 330, 1333, 2121
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 6 cut(s) 20, 392, 2198, 2228, 2255, 2294
HpaII CCGG 5 cut(s) 243, 351, 456, 644, 2445
HphI GGTGA 9 cut(s) 118, 307, 386, 638, 722, 943, 1076, 2650, 2668
Hpy166II GTNNAC 4 cut(s) 253, 1214, 1760, 2659
Hpy188I TCNGA 9 cut(s) 11, 124, 297, 813, 1660, 1733, 2035, 2310, 2701
Hpy8I GTNNAC 4 cut(s) 253, 1214, 1760, 2659
Hpy99I CGWCG 3 cut(s) 67, 254, 2751
HpyAV CCTTC 6 cut(s) 377, 948, 1784, 2614, 2677, 2725
HpyCH4III ACNGT 6 cut(s) 406, 514, 981, 1220, 2305, 2517
HpyCH4IV ACGT 2 cut(s) 14, 102
HpyF10VI GCNNNNNNNGC 8 cut(s) 54, 57, 189, 1044, 1114, 1341, 1848, 2540
HpyF3I CTNAG 8 cut(s) 215, 476, 608, 984, 1034, 1713, 1730, 2700
HpySE526I ACGT 2 cut(s) 14, 102
HspAI GCGC 3 cut(s) 330, 1333, 2121
Ksp22I TGATCA 2 cut(s) 1450, 2713
KspI CCGCGG 1 cut(s) 2385
Kzo9I GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
LweI GCATC 8 cut(s) 1625, 1674, 1971, 2036, 2177, 2199, 2426, 2762
MaeI CTAG 4 cut(s) 1095, 1878, 2024, 2039
MaeII ACGT 2 cut(s) 14, 102
MaeIII GTNAC 9 cut(s) 106, 199, 451, 626, 710, 931, 1609, 1645, 2299
MalI GATC 9 cut(s) 8, 471, 588, 810, 1276, 1452, 2037, 2459, 2715
MboI GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
MfeI CAATTG 2 cut(s) 873, 1371
MflI RGATCY 1 cut(s) 6
MlyI GAGTC 5 cut(s) 140, 625, 928, 1199, 1744
MmeI TCCRAC 2 cut(s) 147, 1167
Mph1103I ATGCAT 1 cut(s) 2192
MroXI GAANNNNTTC 1 cut(s) 966
MslI CAYNNNNRTG 2 cut(s) 192, 2654
MspA1I CMGCKG 4 cut(s) 51, 368, 686, 2384
MspI CCGG 5 cut(s) 243, 351, 456, 644, 2445
MspR9I CCNGG 5 cut(s) 282, 335, 645, 1302, 1755
MunI CAATTG 2 cut(s) 873, 1371
Mva1269I GAATGC 3 cut(s) 1292, 1930, 2493
MvaI CCWGG 4 cut(s) 282, 335, 1302, 1755
MvnI CGCG 3 cut(s) 192, 330, 2384
MwoI GCNNNNNNNGC 8 cut(s) 54, 57, 189, 1044, 1114, 1341, 1848, 2540
NciI CCSGG 1 cut(s) 645
NdeII GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
NheI GCTAGC 1 cut(s) 1877
NlaIV GGNNCC 6 cut(s) 86, 247, 355, 541, 1360, 1862
NmuCI GTSAC 9 cut(s) 106, 199, 451, 626, 710, 931, 1609, 1645, 2299
NsiI ATGCAT 1 cut(s) 2192
NspI RCATGY 4 cut(s) 40, 1354, 1586, 2083
OliI CACNNNNGTG 1 cut(s) 2654
PacI TTAATTAA 1 cut(s) 2781
PagI TCATGA 3 cut(s) 597, 1411, 2766
PaqCI CACCTGC 2 cut(s) 239, 1276
PciI ACATGT 1 cut(s) 1582
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 258
PctI GAATGC 3 cut(s) 1292, 1930, 2493
PdmI GAANNNNTTC 1 cut(s) 966
PfeI GAWTC 6 cut(s) 312, 500, 926, 1082, 1811, 2095
Ple19I CGATCG 1 cut(s) 811
PleI GAGTC 5 cut(s) 140, 624, 928, 1198, 1744
PmaCI CACGTG 1 cut(s) 103
PmlI CACGTG 1 cut(s) 103
PpsI GAGTC 5 cut(s) 140, 624, 928, 1198, 1744
Ppu21I YACGTR 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 446
PscI ACATGT 1 cut(s) 1582
PshBI ATTAAT 1 cut(s) 2777
Psp1406I AACGTT 1 cut(s) 14
Psp5II RGGWCCY 1 cut(s) 446
Psp6I CCWGG 4 cut(s) 280, 333, 1300, 1753
PspCI CACGTG 1 cut(s) 103
PspEI GGTNACC 1 cut(s) 710
PspFI CCCAGC 1 cut(s) 1560
PspGI CCWGG 4 cut(s) 280, 333, 1300, 1753
PspN4I GGNNCC 6 cut(s) 86, 247, 355, 541, 1360, 1862
PspPI GGNCC 7 cut(s) 31, 59, 85, 446, 540, 1427, 1601
PspPPI RGGWCCY 1 cut(s) 446
PstNI CAGNNNCTG 2 cut(s) 371, 1560
PsuI RGATCY 1 cut(s) 6
PvuI CGATCG 1 cut(s) 811
PvuII CAGCTG 2 cut(s) 368, 686
RsaI GTAC 4 cut(s) 68, 606, 1534, 2091
RsaNI GTAC 4 cut(s) 67, 605, 1533, 2090
RseI CAYNNNNRTG 2 cut(s) 192, 2654
SacII CCGCGG 1 cut(s) 2385
SalI GTCGAC 1 cut(s) 251
Sau3AI GATC 9 cut(s) 6, 469, 586, 808, 1274, 1450, 2035, 2457, 2713
Sau96I GGNCC 7 cut(s) 31, 59, 85, 446, 540, 1427, 1601
ScaI AGTACT 1 cut(s) 1534
SchI GAGTC 5 cut(s) 140, 625, 928, 1199, 1744
ScrFI CCNGG 5 cut(s) 282, 335, 645, 1302, 1755
SfaNI GCATC 8 cut(s) 1625, 1674, 1971, 2036, 2177, 2199, 2426, 2762
SfcI CTRYAG 1 cut(s) 977
Sfr303I CCGCGG 1 cut(s) 2385
SgrBI CCGCGG 1 cut(s) 2385
SinI GGWCC 4 cut(s) 85, 446, 540, 1601
SmiMI CAYNNNNRTG 2 cut(s) 192, 2654
SmlI CTYRAG 1 cut(s) 2178
SmoI CTYRAG 1 cut(s) 2178
SspMI CTAG 4 cut(s) 1095, 1878, 2024, 2039
StyD4I CCNGG 5 cut(s) 280, 333, 643, 1300, 1753
StyI CCWWGG 2 cut(s) 735, 1815
TaaI ACNGT 6 cut(s) 406, 514, 981, 1220, 2305, 2517
TaiI ACGT 2 cut(s) 17, 105
TaqI TCGA 8 cut(s) 25, 252, 468, 585, 807, 1277, 1423, 2268
TatI WGTACW 3 cut(s) 604, 1532, 2089
TauI GCSGC 5 cut(s) 54, 57, 60, 1111, 1854
TfiI GAWTC 6 cut(s) 312, 500, 926, 1082, 1811, 2095
TscAI CASTG 8 cut(s) 511, 891, 964, 1126, 1225, 1752, 1879, 2308
TseFI GTSAC 9 cut(s) 106, 199, 451, 626, 710, 931, 1609, 1645, 2299
Tsp45I GTSAC 9 cut(s) 106, 199, 451, 626, 710, 931, 1609, 1645, 2299
TspGWI ACGGA 5 cut(s) 238, 250, 768, 2235, 2444
TspRI CASTG 8 cut(s) 511, 891, 964, 1126, 1225, 1752, 1879, 2308
VpaK11BI GGWCC 4 cut(s) 85, 446, 540, 1601
VspI ATTAAT 1 cut(s) 2777
XagI CCTNNNNNAGG 1 cut(s) 1731
XapI RAATTY 3 cut(s) 290, 1691, 2008
XceI RCATGY 4 cut(s) 40, 1354, 1586, 2083
XcmI CCANNNNNNNNNTGG 2 cut(s) 191, 1557
XmiI GTMKAC 1 cut(s) 252
XmnI GAANNNNTTC 1 cut(s) 966
XspI CTAG 4 cut(s) 1095, 1878, 2024, 2039
ZrmI AGTACT 1 cut(s) 1534
Zsp2I ATGCAT 1 cut(s) 2192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.