pycom06g19820

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0013882
Physical Location & Seq
Forward (+)
23100660 .. 23105662
5003 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2538 bp
ATGGCTGTTTCTGAAAGAGTAGCTGCGGAACGAGGGGAGAATCTGGGGGAATCTATTGGTTACAAAGTTAGGCTTGAAGGGATGAAAGGGCGGGACACCCGGCTTCTTTTTTGCACCACGGGCATATTATTAAGGAGATTACTTGTTGACAGAAAGTTGAGAGGTGTTACACACGTCATTGTTGACGAGATTCATGAACGCGGAATGAATGAAGATTTTCTTCTTATTGTTCTGAAAGAACTTCTCCCTCACCGGCCAGAGTTGAGGTTGATTTTGATGAGTGCCACCCTAAATGCTGAGCTTTTCTCCTCCTACTTTGGTCGTGCTCCAACGATCCATATTCCCGGGTTTACTTACCCAGTCCGAACACATTTTCTGGAGAACATTCTAGAAATGACTGGGTATCGGTTAAATCAATATAATCAGATTGATGATTATGGTCATGAAAAGTCGTGGAAAATGCAGAAACAAGCTCAAGGTTTCAAAAAGAGGAAGAGCCAAATTGCTTCTGCTGTAGAGGATGTACTTGAAGTTGCCGACTTTGGGGAGTACAGTCCAAGGACTCAGGAGTCTCTGTCCTGTTGGAATCCTGACTCTATTGGTTTTAACCTCATTGAGAGTCTTCTTTTACACATAGTCAGGAAAGAACGGCCTGGCGCAATTTTAGTTTTTATGACGGGTTGGGATGACATAAACTCCTTGAAGGATCAACTCCAAGCTCATCCCCTATTAGGAGATCCTAGCAGGGTCCTACTGCTGGCATGTCATGGTTCCATGCCTAGCTCTGAGCAGAAATTGATATTTGATAAGCCAGAAGATGGTGTTCGGAAAATAGTTCTAGCTACAAACATGGCTGAGACTAGTATCACTATCAATGATGTAGTTTTTGTTGTTGATTGTGGAAAGGCGAAAGAGACATCATATGATGCACTAAATAACACTCCCTGTTTGCTTCCTTCCTGGATTTCAAAGGCTGCTTCTCGGCAAAGAAGAGGCAGAGCTGGTCGTGTTCAACCTGGCGAGTGTTACCATCTCTACCCCAGATGTGTATATGATGCCTTTGCTGATTATCAGTTGCCTGAACTTTTGAGGACTCCGTTGCAATCATTATGTTTACAAATCAAAAGTCTACAACTTGGAAGTATTTCAGAGTTCCTATCTAAGGCCTTGCAGCCACCGGAACCTCTGTCGGTTCAAAATGCTGTTGAGTATTTAACGATCATTGGGGCGTTAGATGATAATGAAGACCTGACAGTGTTAGGACGTCACCTGTCAGTGCTTCCCGTTGAGCCTAAGCTTGGAAAAATGCTCATATTGGGGTCTATTTTCAACTGTTTGGATCCAGTAATGACTGTTGTCGCAGGCCTTAGTATGAGAGACCCATTCCTTATGCCGCATGACAAGAAGGATCTTGCAGAGTCCGCAAAAGCACAGTTCTCTGCTCATGATAACAGTGATCATCTTGCTCTTGTCCGAGCTTATGATGGTTGGAGAAGTGCTGAAAGAACAGAATCTGGCTATGAGTACTGCTGGAGAAACTTTCTTTCTGTACAAACTCTGAAAGCCATTGACTCTCTTCGGAAGCAGTTCTTCTTTTTGCTCAAGGATGCAGGTCTGGCTGACCATAATACAGAAAGCTGCAATACATGGAGCCATGATGAGCATCTTGTCCGAGCAGTCATCTGTGCTGGATTATTTCCTGGAATATGCTCTGTCGTGAACAAAGAGAAGTCGATTATGCTTAAAACACAGGAAGATGGACAAGTGATGCTATACTCGAGTTCTGTCAACGGAAGCATACCTAAAATTCCATATCCATGGCTAGTCTTCAATGAAAAGGTGAAAGTGAATTCGGTTTTTCTCCGGGATTCAACTGGTATATCTGATTCTGTGCTCCTTTTGTTCGGGGGCAACATTTCCATGGGTGGCCTGAATGGGCACTTGAAGATGCTGGGAGGATACTTGGAGTTTTTCATGAATCCCGCATTAGCAGATACATATCTTTGCTTAAAGAAGGAGCTTGATGAACTGATTCATAATAAGCTCCTGAATCCCAAGTTGGATATGCAATCACATACAAACCTCCTATCGGCTCTAAGATTACTGGTCTCAGAGGATCGATGTGAGGGTAGATTTGTATTTGGTCGCAAGGTGCCACTGCCCTCAAAGAAGGCAACACAAGAGAAGTTACCAAAGTTAAAAGGCACCATGAAGAAAGTTGCAGAGAACAATAATTACAAAAATCACCTGCAAACATTGCTCACCAGAGCCGGGCATGATGTACCCACCTACAAAACAAAACCATTGAAAAACAATCAGTTCCGTTCCACAGTGATCTTCAATGGGTTGAACTTTGTGGGGAAGCCTTGCAATAGTAAGAAAGAAGCAGAGAAAGATGCAGCTGCAGAAGCTGTACTCTGGTTGAAGGGAGAGAACCATTCTTCGTCCACAGATATTGACCATATGTCGATGCTTCTTAAGAAAAGTAGAAAGACAAGCCAGAAAAGGACCTCATTTGATAGTGCCAAGTGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

846

Amino Acids

95.02

Weight (kDa)

8.62

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 208 - 337 2e-12 Helicase conserved C-terminal domain
WHD_HA2 PF04408 401 - 429 1.2e-08 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 430 - 490 1.2e-14 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 557 - 639 5.1e-19 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 647 - 705 6e-20 C-terminal domain in RNA helicases
dsrm PF00035 746 - 808 2.7e-09 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013882)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48650 AT1G48650
fragaria_vesca FvH4_5g16860
malus_domestica MD06G1222500.v1.1 MD14G1231600.v1.1
prunus_persica Prupe.5G227100_v2.0.a1
pyrus_communis pycom06g19810 pycom06g19820 pycom14g19280
rosa_chinensis RchiOBHm_Chr7g0179211
rosa_laevigata RLG00000005374
rosa_multiflora Rmu_sc0009798.1_g000005 Rmu_sc0021799.1_g000001
rosa_roxburghii Rroxscaffold_3G00274220
rosa_rugosa Rorug06G0422200
rosa_samantha Rh7BG021500
rosa_wichuraiana Rw7G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2256
AasI GACNNNNNNGTC 1 cut(s) 568
AatII GACGTC 1 cut(s) 1267
Acc36I ACCTGC 2 cut(s) 1601, 2256
AccB1I GGYRCC 2 cut(s) 2152, 2204
AccB7I CCANNNNNTGG 1 cut(s) 818
AccI GTMKAC 1 cut(s) 1129
AccII CGCG 1 cut(s) 201
AciI CCGC 6 cut(s) 26, 91, 201, 1394, 1422, 1983
AclWI GGATC 7 cut(s) 328, 714, 731, 1334, 1347, 1416, 2124
AcoI YGGCCR 1 cut(s) 254
AcsI RAATTY 2 cut(s) 1806, 1849
AcyI GRCGYC 1 cut(s) 1264
AfaI GTAC 6 cut(s) 525, 551, 1526, 1551, 2283, 2415
AfiI CCNNNNNNNGG 8 cut(s) 543, 731, 757, 818, 1162, 1298, 2089, 2271
AflII CTTAAG 1 cut(s) 2477
AflIII ACRYGT 1 cut(s) 172
AhdI GACNNNNNGTC 1 cut(s) 2464
AhlI ACTAGT 1 cut(s) 860
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 4 cut(s) 652, 959, 1015, 1699
AjuI GAANNNNNNNTTGG 2 cut(s) 2042, 2074
Alw21I GWGCWC 2 cut(s) 328, 1896
Alw26I GTCTC 5 cut(s) 576, 851, 908, 1371, 2113
AlwI GGATC 7 cut(s) 328, 714, 731, 1334, 1347, 1416, 2124
AlwNI CAGNNNCTG 2 cut(s) 1514, 2411
Ama87I CYCGRG 2 cut(s) 344, 1777
AoxI GGCC 5 cut(s) 254, 650, 1164, 1363, 1927
ApeKI GCWGC 6 cut(s) 23, 974, 1171, 1638, 2399, 2402
ApoI RAATTY 2 cut(s) 1806, 1849
ArsI GACNNNNNNTTYG 2 cut(s) 1111, 1143
Asp700I GAANNNNTTC 4 cut(s) 216, 1144, 1586, 2031
AspLEI GCGC 1 cut(s) 659
AspS9I GGNCC 2 cut(s) 748, 2508
AsuC2I CCSGG 5 cut(s) 100, 345, 346, 1865, 2272
AsuHPI GGTGA 5 cut(s) 242, 1259, 1852, 2237, 2254
AvaI CYCGRG 2 cut(s) 344, 1777
AvaII GGWCC 2 cut(s) 748, 2508
BaeGI GKGCMC 1 cut(s) 1941
BamHI GGATCC 1 cut(s) 1339
BanI GGYRCC 2 cut(s) 2152, 2204
BbsI GAAGAC 3 cut(s) 614, 1251, 1819
Bbv12I GWGCWC 2 cut(s) 328, 1896
BbvI GCAGC 6 cut(s) 10, 961, 1183, 1625, 2389, 2411
BccI CCATC 4 cut(s) 812, 1038, 1478, 1751
BceAI ACGGC 1 cut(s) 665
BciT130I CCWGG 4 cut(s) 654, 961, 1017, 1701
BciVI GTATCC 1 cut(s) 1952
BclI TGATCA 1 cut(s) 1456
BcnI CCSGG 5 cut(s) 100, 345, 346, 1865, 2272
BcoDI GTCTC 5 cut(s) 576, 851, 908, 1371, 2113
BcuI ACTAGT 1 cut(s) 860
BfaI CTAG 6 cut(s) 389, 741, 780, 839, 861, 1823
BfmI CTRYAG 2 cut(s) 513, 2403
BfrI CTTAAG 1 cut(s) 2477
BfuAI ACCTGC 2 cut(s) 1601, 2256
BfuI GTATCC 1 cut(s) 1952
BisI GCNGC 7 cut(s) 24, 975, 1172, 1394, 1639, 2400, 2403
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 7 cut(s) 25, 976, 1173, 1395, 1640, 2401, 2404
BmcAI AGTACT 1 cut(s) 1526
Bme1390I CCNGG 9 cut(s) 100, 345, 346, 654, 961, 1017, 1701, 1865, 2272
Bme18I GGWCC 2 cut(s) 748, 2508
BmeRI GACNNNNNGTC 1 cut(s) 2464
BmeT110I CYCGRG 2 cut(s) 344, 1777
BmgBI CACGTC 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 748, 2508
BmiI GGNNCC 7 cut(s) 749, 772, 1182, 1341, 1652, 2154, 2206
BmrFI CCNGG 9 cut(s) 100, 345, 346, 654, 961, 1017, 1701, 1865, 2272
BmrI ACTGGG 2 cut(s) 353, 408
BmsI GCATC 8 cut(s) 916, 1045, 1597, 1672, 1758, 1938, 2386, 2460
BmuI ACTGGG 2 cut(s) 353, 408
BoxI GACNNNNGTC 1 cut(s) 1355
BpiI GAAGAC 3 cut(s) 614, 1251, 1819
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 2 cut(s) 398, 1552
Bpu10I CCTNAGC 1 cut(s) 1293
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 2 cut(s) 459, 1586
BpuMI CCSGG 5 cut(s) 100, 345, 346, 1865, 2272
Bsa29I ATCGAT 1 cut(s) 2119
BsaBI GATNNNNATC 2 cut(s) 1662, 1998
BsaHI GRCGYC 1 cut(s) 1264
BsaI GGTCTC 2 cut(s) 1371, 2113
BsaJI CCNNGG 5 cut(s) 117, 344, 557, 1817, 1920
BsaWI WCCGGW 1 cut(s) 1177
BsaXI ACNNNNNCTCC 4 cut(s) 560, 590, 680, 710
Bsc4I CCNNNNNNNGG 8 cut(s) 543, 731, 757, 818, 1162, 1298, 2089, 2271
Bse118I RCCGGY 1 cut(s) 252
Bse1I ACTGG 5 cut(s) 359, 403, 1343, 1879, 2109
Bse3DI GCAATG 1 cut(s) 2255
Bse8I GATNNNNATC 2 cut(s) 1662, 1998
BseBI CCWGG 4 cut(s) 654, 961, 1017, 1701
BseCI ATCGAT 1 cut(s) 2119
BseDI CCNNGG 5 cut(s) 117, 344, 557, 1817, 1920
BseGI GGATG 5 cut(s) 87, 526, 691, 721, 1612
BseJI GATNNNNATC 2 cut(s) 1662, 1998
BseLI CCNNNNNNNGG 8 cut(s) 543, 731, 757, 818, 1162, 1298, 2089, 2271
BseMI GCAATG 1 cut(s) 2255
BseMII CTCAG 5 cut(s) 288, 578, 777, 846, 2124
BseNI ACTGG 5 cut(s) 359, 403, 1343, 1879, 2109
BseRI GAGGAG 1 cut(s) 298
BseSI GKGCMC 1 cut(s) 1941
BseXI GCAGC 6 cut(s) 10, 961, 1183, 1625, 2389, 2411
BseYI CCCAGC 1 cut(s) 1951
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 5 cut(s) 256, 652, 1166, 1365, 1929
BshNI GGYRCC 2 cut(s) 2152, 2204
BshVI ATCGAT 1 cut(s) 2119
BsiHKAI GWGCWC 2 cut(s) 328, 1896
BsiHKCI CYCGRG 2 cut(s) 344, 1777
BsiSI CCGG 6 cut(s) 100, 253, 345, 1178, 1864, 2271
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 8 cut(s) 543, 731, 757, 818, 1162, 1298, 2089, 2271
BsmAI GTCTC 5 cut(s) 576, 851, 908, 1371, 2113
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 5 cut(s) 256, 652, 1166, 1365, 1929
Bso31I GGTCTC 2 cut(s) 1371, 2113
BsoBI CYCGRG 2 cut(s) 344, 1777
Bsp1286I GDGCHC 3 cut(s) 328, 1896, 1941
Bsp1407I TGTACA 1 cut(s) 1549
Bsp143I GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
Bsp1720I GCTNAGC 1 cut(s) 297
Bsp19I CCATGG 2 cut(s) 1817, 1920
BspACI CCGC 6 cut(s) 26, 91, 201, 1394, 1422, 1983
BspANI GGCC 5 cut(s) 256, 652, 1166, 1365, 1929
BspCNI CTCAG 5 cut(s) 289, 577, 778, 847, 2123
BspDI ATCGAT 1 cut(s) 2119
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 4 cut(s) 193, 442, 1444, 1974
BspLI GGNNCC 7 cut(s) 749, 772, 1182, 1341, 1652, 2154, 2206
BspMAI CTGCAG 1 cut(s) 2407
BspMI ACCTGC 2 cut(s) 1601, 2256
BspPI GGATC 7 cut(s) 328, 714, 731, 1334, 1347, 1416, 2124
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 2 cut(s) 2152, 2204
BspTI CTTAAG 1 cut(s) 2477
BspTNI GGTCTC 2 cut(s) 1371, 2113
BsrDI GCAATG 1 cut(s) 2255
BsrFI RCCGGY 1 cut(s) 252
BsrGI TGTACA 1 cut(s) 1549
BsrI ACTGG 5 cut(s) 359, 403, 1343, 1879, 2109
BssAI RCCGGY 1 cut(s) 252
BssECI CCNNGG 5 cut(s) 117, 344, 557, 1817, 1920
BssMI GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
BssNI GRCGYC 1 cut(s) 1264
BssT1I CCWWGG 3 cut(s) 557, 1817, 1920
Bst2UI CCWGG 4 cut(s) 654, 961, 1017, 1701
Bst4CI ACNGT 7 cut(s) 554, 1255, 1334, 1354, 1434, 1454, 2332
Bst6I CTCTTC 3 cut(s) 488, 985, 1581
BstACI GRCGYC 1 cut(s) 1264
BstAFI CTTAAG 1 cut(s) 2477
BstAPI GCANNNNNTGC 1 cut(s) 2257
BstAUI TGTACA 1 cut(s) 1549
BstC8I GCNNGC 2 cut(s) 759, 1363
BstDEI CTNAG 9 cut(s) 297, 564, 786, 855, 1161, 1293, 1367, 2096, 2110
BstDSI CCRYGG 3 cut(s) 117, 1817, 1920
BstENI CCTNNNNNAGG 1 cut(s) 1160
BstF5I GGATG 5 cut(s) 87, 526, 691, 721, 1612
BstFNI CGCG 1 cut(s) 201
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 9 cut(s) 336, 709, 739, 1221, 1342, 1411, 1459, 2119, 2337
BstMAI GTCTC 5 cut(s) 576, 851, 908, 1371, 2113
BstMBI GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
BstMWI GCNNNNNNNGC 5 cut(s) 120, 1421, 1616, 2257, 2408
BstNI CCWGG 4 cut(s) 654, 961, 1017, 1701
BstNSI RCATGY 1 cut(s) 765
BstPAI GACNNNNGTC 1 cut(s) 1355
BstSCI CCNGG 9 cut(s) 98, 343, 344, 652, 959, 1015, 1699, 1863, 2270
BstSFI CTRYAG 2 cut(s) 513, 2403
BstSLI GKGCMC 1 cut(s) 1941
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 6 cut(s) 10, 961, 1183, 1625, 2389, 2411
BstV2I GAAGAC 3 cut(s) 614, 1251, 1819
BstX2I RGATCY 3 cut(s) 736, 1339, 1408
BstXI CCANNNNNNTGG 1 cut(s) 1818
BstYI RGATCY 3 cut(s) 736, 1339, 1408
Bsu15I ATCGAT 1 cut(s) 2119
BsuI GTATCC 1 cut(s) 1952
BsuRI GGCC 5 cut(s) 256, 652, 1166, 1365, 1929
BsuTUI ATCGAT 1 cut(s) 2119
BtgI CCRYGG 3 cut(s) 117, 1817, 1920
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 5 cut(s) 87, 526, 691, 721, 1612
BtsI GCAGTG 1 cut(s) 2156
BtsIMutI CAGTG 5 cut(s) 1260, 1281, 1459, 2156, 2337
BveI ACCTGC 2 cut(s) 1601, 2256
Cac8I GCNNGC 2 cut(s) 759, 1363
CaiI CAGNNNCTG 2 cut(s) 1514, 2411
CciI TCATGA 4 cut(s) 193, 442, 1444, 1974
CfoI GCGC 1 cut(s) 659
Cfr10I RCCGGY 1 cut(s) 252
Cfr13I GGNCC 2 cut(s) 748, 2508
Cfr9I CCCGGG 1 cut(s) 344
ClaI ATCGAT 1 cut(s) 2119
Csp6I GTAC 6 cut(s) 524, 550, 1525, 1550, 2282, 2414
CspCI CAANNNNNGTGG 2 cut(s) 2437, 2472
CviQI GTAC 6 cut(s) 524, 550, 1525, 1550, 2282, 2414
DdeI CTNAG 9 cut(s) 297, 564, 786, 855, 1161, 1293, 1367, 2096, 2110
DpnI GATC 9 cut(s) 335, 708, 738, 1220, 1341, 1410, 1458, 2118, 2336
DpnII GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
DrdI GACNNNNNNGTC 1 cut(s) 568
DriI GACNNNNNGTC 1 cut(s) 2464
DseDI GACNNNNNNGTC 1 cut(s) 568
EaeI YGGCCR 1 cut(s) 254
Eam1104I CTCTTC 3 cut(s) 488, 985, 1581
Eam1105I GACNNNNNGTC 1 cut(s) 2464
EarI CTCTTC 3 cut(s) 488, 985, 1581
Eco130I CCWWGG 3 cut(s) 557, 1817, 1920
Eco147I AGGCCT 2 cut(s) 1166, 1365
Eco31I GGTCTC 2 cut(s) 1371, 2113
Eco47I GGWCC 2 cut(s) 748, 2508
Eco88I CYCGRG 2 cut(s) 344, 1777
EcoNI CCTNNNNNAGG 1 cut(s) 1160
EcoO109I RGGNCCY 2 cut(s) 748, 2508
EcoRI GAATTC 1 cut(s) 1849
EcoRII CCWGG 4 cut(s) 652, 959, 1015, 1699
EcoT14I CCWWGG 3 cut(s) 557, 1817, 1920
ErhI CCWWGG 3 cut(s) 557, 1817, 1920
FalI AAGNNNNNCTT 6 cut(s) 57, 89, 204, 236, 1574, 1606
FaqI GGGAC 1 cut(s) 107
FauI CCCGC 2 cut(s) 84, 1990
FauNDI CATATG 2 cut(s) 922, 2463
FbaI TGATCA 1 cut(s) 1456
FblI GTMKAC 1 cut(s) 1129
Fnu4HI GCNGC 7 cut(s) 24, 975, 1172, 1394, 1639, 2400, 2403
FokI GGATG 5 cut(s) 94, 533, 698, 708, 1619
Fsp4HI GCNGC 7 cut(s) 24, 975, 1172, 1394, 1639, 2400, 2403
FspBI CTAG 6 cut(s) 389, 741, 780, 839, 861, 1823
GlaI GCGC 1 cut(s) 658
GluI GCNGC 7 cut(s) 24, 975, 1172, 1394, 1639, 2400, 2403
GsaI CCCAGC 1 cut(s) 1955
GsuI CTGGAG 2 cut(s) 398, 1552
HaeIII GGCC 5 cut(s) 256, 652, 1166, 1365, 1929
HapII CCGG 6 cut(s) 100, 253, 345, 1178, 1864, 2271
HhaI GCGC 1 cut(s) 659
Hin1I GRCGYC 1 cut(s) 1264
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HincII GTYRAC 3 cut(s) 148, 184, 1789
HindII GTYRAC 3 cut(s) 148, 184, 1789
HindIII AAGCTT 1 cut(s) 1295
HpaII CCGG 6 cut(s) 100, 253, 345, 1178, 1864, 2271
HphI GGTGA 5 cut(s) 242, 1259, 1852, 2237, 2254
Hpy166II GTNNAC 8 cut(s) 148, 184, 351, 1115, 1130, 1720, 1789, 2448
Hpy8I GTNNAC 8 cut(s) 148, 184, 351, 1115, 1130, 1720, 1789, 2448
HpyAV CCTTC 7 cut(s) 71, 697, 966, 1399, 2008, 2164, 2419
HpyCH4III ACNGT 7 cut(s) 554, 1255, 1334, 1354, 1434, 1454, 2332
HpyCH4IV ACGT 2 cut(s) 174, 1264
HpyF10VI GCNNNNNNNGC 5 cut(s) 120, 1421, 1616, 2257, 2408
HpyF3I CTNAG 9 cut(s) 297, 564, 786, 855, 1161, 1293, 1367, 2096, 2110
HpySE526I ACGT 2 cut(s) 174, 1264
Hsp92I GRCGYC 1 cut(s) 1264
HspAI GCGC 1 cut(s) 657
Ksp22I TGATCA 1 cut(s) 1456
Kzo9I GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 5 cut(s) 331, 1650, 1899, 2017, 2049
Lsp1109I GCAGC 6 cut(s) 10, 961, 1183, 1625, 2389, 2411
LweI GCATC 8 cut(s) 916, 1045, 1597, 1672, 1758, 1938, 2386, 2460
MaeI CTAG 6 cut(s) 389, 741, 780, 839, 861, 1823
MaeII ACGT 2 cut(s) 174, 1264
MaeIII GTNAC 5 cut(s) 59, 166, 1025, 1265, 2187
MalI GATC 9 cut(s) 335, 708, 738, 1220, 1341, 1410, 1458, 2118, 2336
MboI GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
MflI RGATCY 3 cut(s) 736, 1339, 1408
MhlI GDGCHC 3 cut(s) 328, 1896, 1941
MluCI AATT 6 cut(s) 501, 660, 794, 1806, 1849, 2233
MlyI GAGTC 7 cut(s) 556, 578, 587, 628, 1087, 1427, 1565
MmeI TCCRAC 4 cut(s) 353, 563, 1469, 2040
MroXI GAANNNNTTC 4 cut(s) 216, 1144, 1586, 2031
MseI TTAA 9 cut(s) 131, 410, 606, 1214, 1743, 2009, 2198, 2478, 2536
MslI CAYNNNNRTG 2 cut(s) 1816, 1919
MspA1I CMGCKG 1 cut(s) 2402
MspCI CTTAAG 1 cut(s) 2477
MspI CCGG 6 cut(s) 100, 253, 345, 1178, 1864, 2271
MspR9I CCNGG 9 cut(s) 100, 345, 346, 654, 961, 1017, 1701, 1865, 2272
MvaI CCWGG 4 cut(s) 654, 961, 1017, 1701
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 5 cut(s) 120, 1421, 1616, 2257, 2408
NciI CCSGG 5 cut(s) 100, 345, 346, 1865, 2272
NcoI CCATGG 2 cut(s) 1817, 1920
NdeI CATATG 2 cut(s) 922, 2463
NdeII GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
NlaIV GGNNCC 7 cut(s) 749, 772, 1182, 1341, 1652, 2154, 2206
NmeAIII GCCGAG 1 cut(s) 961
NmuCI GTSAC 1 cut(s) 1265
NspI RCATGY 1 cut(s) 765
PaeR7I CTCGAG 1 cut(s) 1777
PagI TCATGA 4 cut(s) 193, 442, 1444, 1974
PaqCI CACCTGC 1 cut(s) 2256
PceI AGGCCT 2 cut(s) 1166, 1365
PciSI GCTCTTC 1 cut(s) 488
PdmI GAANNNNTTC 4 cut(s) 216, 1144, 1586, 2031
PflMI CCANNNNNTGG 1 cut(s) 818
PfoI TCCNGGA 3 cut(s) 959, 1699, 1863
PkrI GCNGC 7 cut(s) 25, 976, 1173, 1395, 1640, 2401, 2404
PleI GAGTC 7 cut(s) 556, 577, 587, 627, 1087, 1426, 1565
PpsI GAGTC 7 cut(s) 556, 577, 587, 627, 1087, 1426, 1565
PpuMI RGGWCCY 2 cut(s) 748, 2508
PshAI GACNNNNGTC 1 cut(s) 1355
Psp5II RGGWCCY 2 cut(s) 748, 2508
Psp6I CCWGG 4 cut(s) 652, 959, 1015, 1699
PspFI CCCAGC 1 cut(s) 1951
PspGI CCWGG 4 cut(s) 652, 959, 1015, 1699
PspN4I GGNNCC 7 cut(s) 749, 772, 1182, 1341, 1652, 2154, 2206
PspPI GGNCC 2 cut(s) 748, 2508
PspPPI RGGWCCY 2 cut(s) 748, 2508
PspXI VCTCGAGB 1 cut(s) 1777
PsrI GAACNNNNNNTAC 2 cut(s) 2219, 2251
PstI CTGCAG 1 cut(s) 2407
PstNI CAGNNNCTG 2 cut(s) 1514, 2411
PsuI RGATCY 3 cut(s) 736, 1339, 1408
PvuII CAGCTG 1 cut(s) 2402
RsaI GTAC 6 cut(s) 525, 551, 1526, 1551, 2283, 2415
RsaNI GTAC 6 cut(s) 524, 550, 1525, 1550, 2282, 2414
RseI CAYNNNNRTG 2 cut(s) 1816, 1919
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 9 cut(s) 131, 410, 606, 1214, 1743, 2009, 2198, 2478, 2536
SatI GCNGC 7 cut(s) 24, 975, 1172, 1394, 1639, 2400, 2403
Sau3AI GATC 9 cut(s) 333, 706, 736, 1218, 1339, 1408, 1456, 2116, 2334
Sau96I GGNCC 2 cut(s) 748, 2508
ScaI AGTACT 1 cut(s) 1526
SchI GAGTC 7 cut(s) 556, 578, 587, 628, 1087, 1427, 1565
ScrFI CCNGG 9 cut(s) 100, 345, 346, 654, 961, 1017, 1701, 1865, 2272
SduI GDGCHC 3 cut(s) 328, 1896, 1941
SfaNI GCATC 8 cut(s) 916, 1045, 1597, 1672, 1758, 1938, 2386, 2460
SfcI CTRYAG 2 cut(s) 513, 2403
Sfr274I CTCGAG 1 cut(s) 1777
SinI GGWCC 2 cut(s) 748, 2508
SlaI CTCGAG 1 cut(s) 1777
SmaI CCCGGG 1 cut(s) 346
SmiMI CAYNNNNRTG 2 cut(s) 1816, 1919
SmlI CTYRAG 4 cut(s) 474, 1601, 1777, 2477
SmoI CTYRAG 4 cut(s) 474, 1601, 1777, 2477
SpeI ACTAGT 1 cut(s) 860
Sse9I AATT 6 cut(s) 501, 660, 794, 1806, 1849, 2233
SseBI AGGCCT 2 cut(s) 1166, 1365
SsiI CCGC 6 cut(s) 26, 91, 201, 1394, 1422, 1983
SspMI CTAG 6 cut(s) 389, 741, 780, 839, 861, 1823
StuI AGGCCT 2 cut(s) 1166, 1365
StyD4I CCNGG 9 cut(s) 98, 343, 344, 652, 959, 1015, 1699, 1863, 2270
StyI CCWWGG 3 cut(s) 557, 1817, 1920
TaaI ACNGT 7 cut(s) 554, 1255, 1334, 1354, 1434, 1454, 2332
TaiI ACGT 2 cut(s) 177, 1267
TaqI TCGA 4 cut(s) 1733, 1778, 2119, 2468
TasI AATT 6 cut(s) 501, 660, 794, 1806, 1849, 2233
TatI WGTACW 5 cut(s) 523, 549, 1524, 1549, 2413
TauI GCSGC 1 cut(s) 1396
Tru1I TTAA 9 cut(s) 131, 410, 606, 1214, 1743, 2009, 2198, 2478, 2536
Tru9I TTAA 9 cut(s) 131, 410, 606, 1214, 1743, 2009, 2198, 2478, 2536
TscAI CASTG 5 cut(s) 1260, 1281, 1459, 2163, 2337
TseFI GTSAC 1 cut(s) 1265
TseI GCWGC 6 cut(s) 23, 974, 1171, 1638, 2399, 2402
Tsp45I GTSAC 1 cut(s) 1265
TspGWI ACGGA 3 cut(s) 1086, 1806, 2312
TspMI CCCGGG 1 cut(s) 344
TspRI CASTG 5 cut(s) 1260, 1281, 1459, 2163, 2337
Van91I CCANNNNNTGG 1 cut(s) 818
Vha464I CTTAAG 1 cut(s) 2477
VpaK11BI GGWCC 2 cut(s) 748, 2508
XagI CCTNNNNNAGG 1 cut(s) 1160
XapI RAATTY 2 cut(s) 1806, 1849
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 1 cut(s) 765
XhoI CTCGAG 1 cut(s) 1777
XmaI CCCGGG 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 1129
XmnI GAANNNNTTC 4 cut(s) 216, 1144, 1586, 2031
XspI CTAG 6 cut(s) 389, 741, 780, 839, 861, 1823
ZraI GACGTC 1 cut(s) 1265
ZrmI AGTACT 1 cut(s) 1526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.