pycom06g21630

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011305
Physical Location & Seq
Forward (+)
24201987 .. 24204944
2958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g21630.2

Sequence Viewer

Length: 876 bp
ATGACCACTGGCACTGAAGAAGTAGTATTAAAAGAAGAAGAAGAAAGAATGGTTAGGATGGGATCGTACGGAGGAATGGTTAGGCTGCTGACGGTGACGGCAACGGCAAAATCAGAGGAAGAGGAAGAAGAAAAAGAAAAGGAAGAGTGTTCATATGAAGCAGCTGCGGAGGAGATAATGCTGCTGTGGGGAATCCAACAGCCAACTCTGTCTAAGCCGAACGCTTTCGTCTCTCAAGCTTGTCTTGAACTGAAACTGGATGCGTGTGGCCATTCACTCTCCATTTTCCAGTCCCCTTCTTCCTTGAATACACCTGGGGTCACTGGTGCTGTTATGTGGGACAGTGGAGTTGTATTGGGGAAGTTTCTAGAGCATGCTTCAGACGCGGAGTTACTACCTCTTCAAGGGAAGAAAGTTGTTGAACTCGGCTCTGGCTGTGGATTAGTCGGCTGCATTGCAGCTCTTCTAGGCGGTCTAGTTGTGCTCACAGATCTTCCTGATAGACTGAGACTACTGAGAAAGAACATTGAAGTCAATCTGAGACATGGAGACATGCGAGGTTCAGCTAAAGTGATGGAATTCACATGGGGAGATGATCCTGATCCAGAACTGACTGAACCTCCACCTGATTTGGTGCTAGGCTCTGATGTAATCTATAGTGAGGAAGCAGTGTTGGATTTGTTGTCTGCATTACAGCAACTCTCCGGAGGCGAAACGACAATCTTTTTGGCTGGAGAACTTCGAAATGATGCTGTCCTTGAATACTTCTTGGAGTGTGCAATGAAGGATTTTGTAATTGGGCGTTTGGACCAGAGACAGTGGCATCCAGAATATTGCAGCAGCCGTGTTGTGTTATACGTTCTCGTGAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

292

Amino Acids

31.97

Weight (kDa)

4.56

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73320 AT1G73320
fragaria_vesca FvH4_5g18590 FvH4_5g18590 FvH4_5g18590 FvH4_5g18590
malus_domestica MD06G1241200.v1.1
prunus_persica Prupe.5G245300_v2.0.a1
pyrus_communis pycom06g21630
rosa_chinensis RchiOBHm_Chr7g0176921
rosa_laevigata RLG00000005573
rosa_multiflora Rmu_sc0020732.1_g000001
rosa_roxburghii Rroxscaffold_3G00276290
rosa_rugosa Rorug06G0401800 Rorug06G0401900
rosa_samantha Rh7AG001400 Rh7BG001700 Rh7CG001200 Rh7DG001500
rosa_wichuraiana Rw7G000100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 386
AccIII TCCGGA 1 cut(s) 704
AciI CCGC 3 cut(s) 167, 386, 471
AclWI GGATC 3 cut(s) 70, 590, 596
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 1 cut(s) 578
AcuI CTGAAG 2 cut(s) 36, 363
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 1 cut(s) 404
AgsI TTSAA 6 cut(s) 248, 307, 404, 422, 530, 761
AjnI CCWGG 1 cut(s) 313
AluBI AGCT 4 cut(s) 164, 239, 461, 566
AluI AGCT 4 cut(s) 164, 239, 461, 566
Alw21I GWGCWC 1 cut(s) 486
Alw26I GTCTC 5 cut(s) 235, 502, 535, 543, 808
AlwI GGATC 3 cut(s) 70, 590, 596
Aor13HI TCCGGA 1 cut(s) 704
AoxI GGCC 1 cut(s) 268
ApeKI GCWGC 8 cut(s) 85, 161, 164, 181, 450, 458, 837, 840
ApoI RAATTY 1 cut(s) 578
ArsI GACNNNNNNTTYG 2 cut(s) 709, 741
Asp700I GAANNNNTTC 1 cut(s) 224
AspS9I GGNCC 1 cut(s) 808
AsuHPI GGTGA 1 cut(s) 106
AsuII TTCGAA 1 cut(s) 742
AvaII GGWCC 1 cut(s) 808
BalI TGGCCA 1 cut(s) 270
BauI CACGAG 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 8 cut(s) 72, 151, 168, 173, 437, 470, 849, 852
BccI CCATC 2 cut(s) 52, 568
BceAI ACGGC 3 cut(s) 114, 120, 828
BciT130I CCWGG 1 cut(s) 315
BcoDI GTCTC 5 cut(s) 235, 502, 535, 543, 808
BfaI CTAG 4 cut(s) 368, 467, 476, 638
BfmI CTRYAG 1 cut(s) 655
BglII AGATCT 1 cut(s) 490
BisI GCNGC 8 cut(s) 86, 162, 165, 182, 451, 459, 838, 841
BlsI GCNGC 8 cut(s) 87, 163, 166, 183, 452, 460, 839, 842
Bme1390I CCNGG 1 cut(s) 315
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 808
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 250, 739, 832
BpmI CTGGAG 1 cut(s) 753
Bpu14I TTCGAA 1 cut(s) 742
BpuEI CTTGAG 1 cut(s) 219
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 704
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 604, 634
Bsc4I CCNNNNNNNGG 1 cut(s) 404
Bse1I ACTGG 4 cut(s) 13, 261, 289, 328
Bse3DI GCAATG 2 cut(s) 453, 786
Bse8I GATNNNNATC 1 cut(s) 600
BseAI TCCGGA 1 cut(s) 704
BseBI CCWGG 1 cut(s) 315
BseDI CCNNGG 1 cut(s) 314
BseGI GGATG 3 cut(s) 63, 265, 823
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 1 cut(s) 404
BseMI GCAATG 2 cut(s) 453, 786
BseMII CTCAG 3 cut(s) 497, 506, 530
BseNI ACTGG 4 cut(s) 13, 261, 289, 328
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 8 cut(s) 72, 151, 168, 173, 437, 470, 849, 852
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 1 cut(s) 705
BsiWI CGTACG 1 cut(s) 66
BslFI GGGAC 2 cut(s) 277, 353
BslI CCNNNNNNNGG 1 cut(s) 404
BsmAI GTCTC 5 cut(s) 235, 502, 535, 543, 808
BsmBI CGTCTC 1 cut(s) 235
BsmFI GGGAC 2 cut(s) 277, 353
BsnI GGCC 1 cut(s) 270
Bsp119I TTCGAA 1 cut(s) 742
Bsp1286I GDGCHC 1 cut(s) 486
Bsp13I TCCGGA 1 cut(s) 704
Bsp143I GATC 4 cut(s) 62, 490, 595, 601
BspACI CCGC 3 cut(s) 167, 386, 471
BspANI GGCC 1 cut(s) 270
BspCNI CTCAG 3 cut(s) 498, 507, 531
BspEI TCCGGA 1 cut(s) 704
BspFNI CGCG 1 cut(s) 386
BspPI GGATC 3 cut(s) 70, 590, 596
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 1 cut(s) 742
BsrDI GCAATG 2 cut(s) 453, 786
BsrI ACTGG 4 cut(s) 13, 261, 289, 328
BssECI CCNNGG 1 cut(s) 314
BssMI GATC 4 cut(s) 62, 490, 595, 601
BssSI CACGAG 1 cut(s) 863
Bst2BI CACGAG 1 cut(s) 863
Bst2UI CCWGG 1 cut(s) 315
Bst4CI ACNGT 3 cut(s) 94, 344, 819
Bst6I CTCTTC 4 cut(s) 114, 138, 405, 468
BstBI TTCGAA 1 cut(s) 742
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 4 cut(s) 213, 506, 515, 539
BstENI CCTNNNNNAGG 1 cut(s) 402
BstF5I GGATG 3 cut(s) 63, 265, 823
BstFNI CGCG 1 cut(s) 386
BstKTI GATC 4 cut(s) 65, 493, 598, 604
BstMAI GTCTC 5 cut(s) 235, 502, 535, 543, 808
BstMBI GATC 4 cut(s) 62, 490, 595, 601
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstNI CCWGG 1 cut(s) 315
BstNSI RCATGY 2 cut(s) 377, 556
BstSCI CCNGG 1 cut(s) 313
BstSFI CTRYAG 1 cut(s) 655
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 8 cut(s) 72, 151, 168, 173, 437, 470, 849, 852
BstX2I RGATCY 1 cut(s) 490
BstYI RGATCY 1 cut(s) 490
BsuRI GGCC 1 cut(s) 270
BtsCI GGATG 3 cut(s) 63, 265, 823
BtsI GCAGTG 1 cut(s) 675
BtsIMutI CAGTG 6 cut(s) 6, 12, 321, 349, 675, 824
Cac8I GCNNGC 1 cut(s) 375
Cfr13I GGNCC 1 cut(s) 808
CseI GACGC 1 cut(s) 392
Csp6I GTAC 1 cut(s) 67
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviAII CATG 4 cut(s) 374, 545, 553, 585
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 4 cut(s) 213, 506, 515, 539
DpnI GATC 4 cut(s) 64, 492, 597, 603
DpnII GATC 4 cut(s) 62, 490, 595, 601
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 4 cut(s) 114, 138, 405, 468
EarI CTCTTC 4 cut(s) 114, 138, 405, 468
Eco47I GGWCC 1 cut(s) 808
Eco57I CTGAAG 2 cut(s) 36, 363
EcoNI CCTNNNNNAGG 1 cut(s) 402
EcoRI GAATTC 1 cut(s) 578
EcoRII CCWGG 1 cut(s) 313
Esp3I CGTCTC 1 cut(s) 235
FaeI CATG 4 cut(s) 377, 548, 556, 588
FaiI YATR 9 cut(s) 154, 156, 335, 375, 546, 554, 586, 657, 856
FalI AAGNNNNNCTT 2 cut(s) 228, 260
FaqI GGGAC 2 cut(s) 277, 353
FatI CATG 4 cut(s) 373, 544, 552, 584
FauNDI CATATG 1 cut(s) 154
Fnu4HI GCNGC 8 cut(s) 86, 162, 165, 182, 451, 459, 838, 841
FokI GGATG 3 cut(s) 70, 272, 810
Fsp4HI GCNGC 8 cut(s) 86, 162, 165, 182, 451, 459, 838, 841
FspBI CTAG 4 cut(s) 368, 467, 476, 638
GluI GCNGC 8 cut(s) 86, 162, 165, 182, 451, 459, 838, 841
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 1 cut(s) 270
HapII CCGG 1 cut(s) 705
HgaI GACGC 1 cut(s) 392
Hin1II CATG 4 cut(s) 377, 548, 556, 588
HindIII AAGCTT 1 cut(s) 237
HinfI GANTC 1 cut(s) 192
HpaII CCGG 1 cut(s) 705
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 4 cut(s) 115, 382, 540, 646
Hpy188III TCNNGA 8 cut(s) 245, 368, 497, 599, 605, 705, 827, 865
HpyAV CCTTC 2 cut(s) 306, 778
HpyCH4III ACNGT 3 cut(s) 94, 344, 819
HpyCH4IV ACGT 1 cut(s) 858
HpyCH4V TGCA 5 cut(s) 453, 458, 689, 779, 837
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 4 cut(s) 213, 506, 515, 539
HpySE526I ACGT 1 cut(s) 858
Hsp92II CATG 4 cut(s) 377, 548, 556, 588
Kpn2I TCCGGA 1 cut(s) 704
Kzo9I GATC 4 cut(s) 62, 490, 595, 601
LguI GCTCTTC 1 cut(s) 468
Lsp1109I GCAGC 8 cut(s) 72, 151, 168, 173, 437, 470, 849, 852
LweI GCATC 3 cut(s) 250, 739, 832
MaeI CTAG 4 cut(s) 368, 467, 476, 638
MaeII ACGT 1 cut(s) 858
MaeIII GTNAC 3 cut(s) 94, 319, 390
MalI GATC 4 cut(s) 64, 492, 597, 603
MboI GATC 4 cut(s) 62, 490, 595, 601
MflI RGATCY 1 cut(s) 490
MhlI GDGCHC 1 cut(s) 486
MlsI TGGCCA 1 cut(s) 270
MluCI AATT 2 cut(s) 578, 795
MluNI TGGCCA 1 cut(s) 270
MmeI TCCRAC 2 cut(s) 220, 654
Mox20I TGGCCA 1 cut(s) 270
MroI TCCGGA 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 1 cut(s) 270
MseI TTAA 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 315
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 1 cut(s) 383
NdeI CATATG 1 cut(s) 154
NdeII GATC 4 cut(s) 62, 490, 595, 601
NlaIII CATG 4 cut(s) 377, 548, 556, 588
NmeAIII GCCGAG 1 cut(s) 405
NmuCI GTSAC 2 cut(s) 94, 319
NspI RCATGY 2 cut(s) 377, 556
NspV TTCGAA 1 cut(s) 742
PaeI GCATGC 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 468
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 192
Pfl23II CGTACG 1 cut(s) 66
PkrI GCNGC 8 cut(s) 87, 163, 166, 183, 452, 460, 839, 842
Psp6I CCWGG 1 cut(s) 313
PspGI CCWGG 1 cut(s) 313
PspLI CGTACG 1 cut(s) 66
PspPI GGNCC 1 cut(s) 808
PsuI RGATCY 1 cut(s) 490
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 8 cut(s) 86, 162, 165, 182, 451, 459, 838, 841
Sau3AI GATC 4 cut(s) 62, 490, 595, 601
Sau96I GGNCC 1 cut(s) 808
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 486
SfaNI GCATC 3 cut(s) 250, 739, 832
SfcI CTRYAG 1 cut(s) 655
SfuI TTCGAA 1 cut(s) 742
SinI GGWCC 1 cut(s) 808
SmlI CTYRAG 1 cut(s) 234
SmoI CTYRAG 1 cut(s) 234
SphI GCATGC 1 cut(s) 377
Sse9I AATT 2 cut(s) 578, 795
SsiI CCGC 3 cut(s) 167, 386, 471
SspI AATATT 1 cut(s) 833
SspMI CTAG 4 cut(s) 368, 467, 476, 638
StyD4I CCNGG 1 cut(s) 313
TaaI ACNGT 3 cut(s) 94, 344, 819
TaiI ACGT 1 cut(s) 861
TaqI TCGA 1 cut(s) 742
TasI AATT 2 cut(s) 578, 795
TfiI GAWTC 1 cut(s) 192
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 6 cut(s) 13, 19, 328, 349, 675, 824
TseFI GTSAC 2 cut(s) 94, 319
TseI GCWGC 8 cut(s) 85, 161, 164, 181, 450, 458, 837, 840
Tsp45I GTSAC 2 cut(s) 94, 319
TspDTI ATGAA 3 cut(s) 141, 171, 797
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 6 cut(s) 13, 19, 328, 349, 675, 824
VpaK11BI GGWCC 1 cut(s) 808
XagI CCTNNNNNAGG 1 cut(s) 402
XapI RAATTY 1 cut(s) 578
XbaI TCTAGA 1 cut(s) 367
XceI RCATGY 2 cut(s) 377, 556
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 4 cut(s) 368, 467, 476, 638
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.