Rorug06G0401900

Lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011305
Physical Location & Seq
Forward (+)
54658390 .. 54662588
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0401900.1

Sequence Viewer

Length: 2718 bp
ATGCCTCGCAAGCCCACCGCCACCATCTTCCTAGGGTTTCTCCTCCTCCTTACATTCTGCCATGCCGATCCGGGTTCCAATTGCCCCAAAACCAGCCCTCTCGTCAACTTGGAATCGCAATTCAAAATGCTCCAGCACCAGCTCAGAGGCTCCGTTAAGATAATCGACGATTGCTCCTTCCAGGTTTCCAATTTCGACATGCTCTCCGGCTCCGACGTCCACTGGTGGGGCGCCGTCGCCCCCGATTTCAATAATCTCACCGCCGGCTTCGTAATCTCCGACCAGAAGCTCAACGAGACATACAAAAGCGCGAGCTTCACTGTGCGCCTGAGGGACAATGTCACCTGGAATCAGATCCAAGTCCTCGCCGTCTGGGACCTCCCCACCTCCTCCGACTTCGGTCACATCGTGCTCCGGGATGTAGTAAACAGATCGTCCGGCAGCTCCGATTTGGCTCCTTCTCCGTCGCCGGCGAGCGATTCAGGGAATGGGACCGGTCAGGTTCACACTGAGCCGACTATGTTGGTGAATTGCAAGAGTTTGTCCGACGATTTTAGGGTTAGATGGACACTGCGTCCTGAGGAGAATGTGATTGACATTGGGCTGGAGGCTGCCACCGGGTCGACGAATTACATGGCTTTCGGGTGGGCCCGCCCGAATATCACACACCAGTTTATGCTCGGAGCTGACGTGGCCGTCGCCGGATTCAAGGAGGACGGAATGCCGTTTGTGGATGATTTTTACATCACAAAGTACAGCGAGTGCACCGTGTACAAGGACGGCTCCGTCAAAGGGGTGTGCCCCGATACAAGATACGAAGGGACGGCCCCGAATGGCTTGGTGAACAATACCAAATTGGTTTACGGCCACCGGAGGGACGCCGTGTCGTTTATTAGGTACCAGAGGCCGTTGGAATCAGCTGATAAAAAGTATGATTTGGCGGTGAAGCATACCGAGAAAAGGACGGTGATTTGGGCCGTGGGGCCGATCAGGCCACCGGATACTCTTCAGCCTTACTATCTTCCCCAAAACCATGGGGGACCCCAGGATGTGGTTTATGGCCATTTGGAGCTCAATGTGTCTGAGCATGTGGATGACTGTTATGGTCCTATAGAGGCCGATGACAAGGAGGATCAGCACCTCATTATTGCGGATGCAAAGGCCCCGCTTGTGGTTACTTCCGGCCAGGCAGTGCATTATCCAGACCCTCCGAACCCTTCAAAGGTTCTTTACATTAACAAGAAAGAGGCTCCGGTGTTGAGAGTTGAAAGAGGGGTGCCGGTCACGTTTTCAATACAAGCTGGGCATGATGTTGCTCTCTACATTACTTCAGATCCTCTTGGTGGGAATGCTACACTGAGGAATGCAAGCGAGACTGTGTATGCTGGAGGATCGGAAGCTCAAGGAGTTCAAGCCAGTCCCAAGGAGTTAATTTGGGCACCAGACAGGAATACCCCAGACCTAGTGTACTATCAATCTCTTTATGACCAGAAAATGGGATGGAAGGTACAGGTGGTTGATGGGGGTCTGTCAGATATGTATAATAACAGTGTGATTTTGGATGATCAGCAAGTTACCTTATTTTGGACACTGGCACATGATTCTATATCTATTGCAGTCCGGGGTGAGAAGAAAAGTGGTTTCGTGGCAATAGGATTTGGTAGGGGAATGGTGAACAGCTATGCTTATGTGGGTTGGATTGATAATATTGGCAAAGGGCGGGTAAATACTTACTGGATTGATGGAAAGGATGCCTCGAGTGTGCATCCAACACATGAGAATTTGACATACGTGAGGTGCAGGTCAGAAAATGGGATCATTACGTTCGAGTTCACTCGTCCTTTGAAACCATCATGTGGTAAGAGTGATAAGCCCGAGTGTAAAAACATAATTGATCCAACTACTCCTCTTAAAATTATATGGGCAATGGGTGCTACATGGACCGATGATCATCTTAGTGATCAAAACATGCATTTTGCCACCAGCAGTAGGCCTATAAGGGTGCTGCTTATGCGTGGTTCTGCAGAGGCAGAGCAGGATTTACAGCCAGTATTAGCAGTGCATGGATTTATGATGTTTCTCGCTTGGGCTATCTTGCTTCCTGGTGGAATACTGGCGGCTAGATACTTAAAACATGTTAAGGGTGATGGTTGGTACCGGATTCATGTATACTTGCAGTACTCAGGTTTGGTAATCGTTCTACTTGCCCTTCTTTTTGCCGTTGCTGAGCTCCGTGGTTTCTTTTTCGGCTCATTACATGTTAAATTTGGAACTACGGCTATACTTTTGGTTTGTATGCAACCAGTGAATGCATACCTAAGGCCAAAGAGACCAAATAATGGAGAGCTGGTTTCCTCTAAAAGGCTTCTATGGGAGTATCTTCATGTGATCGGGGGCAGATCTGCCATTGTAGTAGGTTTTGGAGCACTTTTCACAGGACTGAAGCATTTAGGTGATAGATATGGCGGCGAAAATGTTGATGGACTAAATTTAGCTTTGATAGTTTGGTTCTTGATCTGTGCATTGATAGTAATATATCTGGAATACTATGAAAGGCAAAGAAGGAGGAATAGAAGTATCGGCAGAAGCAATTGGGTGCTGGGGAACCTTGAGGAGGATGACTCTGTTGATTTGTTGAGCCCAAATGGAATACATTCAGAGAAAGAATCACATACATCAGGAAGAATGGAAGTTCAATTAGAGCCTCTGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

905

Amino Acids

100.62

Weight (kDa)

6.15

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DM13 PF10517 40 - 137 4e-15 Electron transfer DM13
DOMON PF03351 183 - 328 9.9e-12 DOMON domain
At5g54830 PF25489 382 - 507 1.1e-69 At5g54830-like domain
DOMON PF03351 521 - 644 4e-25 DOMON domain
Cytochrom_C_asm PF01578 684 - 851 3.9e-06 Cytochrome C assembly protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011305)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G73320 AT1G73320
fragaria_vesca FvH4_5g18590 FvH4_5g18590 FvH4_5g18590 FvH4_5g18590
malus_domestica MD06G1241200.v1.1
prunus_persica Prupe.5G245300_v2.0.a1
pyrus_communis pycom06g21630
rosa_chinensis RchiOBHm_Chr7g0176921
rosa_laevigata RLG00000005573
rosa_multiflora Rmu_sc0020732.1_g000001
rosa_roxburghii Rroxscaffold_3G00276290
rosa_rugosa Rorug06G0401800 Rorug06G0401900
rosa_samantha Rh7AG001400 Rh7BG001700 Rh7CG001200 Rh7DG001500
rosa_wichuraiana Rw7G000100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 695, 785
AatII GACGTC 1 cut(s) 219
Acc36I ACCTGC 1 cut(s) 1791
Acc65I GGTACC 2 cut(s) 897, 2154
AccB1I GGYRCC 5 cut(s) 230, 897, 1276, 1438, 2154
AccB7I CCANNNNNTGG 4 cut(s) 1051, 1495, 1856, 2339
AccI GTMKAC 2 cut(s) 623, 2169
AccII CGCG 1 cut(s) 311
AciI CCGC 9 cut(s) 18, 261, 652, 941, 1151, 1166, 1720, 2117, 2466
AclWI GGATC 7 cut(s) 62, 349, 1140, 1328, 1399, 1823, 1889
AcoI YGGCCR 4 cut(s) 693, 865, 1060, 1183
AcsI RAATTY 3 cut(s) 1780, 2264, 2488
AcuI CTGAAG 3 cut(s) 992, 1314, 2462
AcyI GRCGYC 3 cut(s) 216, 231, 879
AdeI CACNNNGTG 1 cut(s) 409
AfaI GTAC 7 cut(s) 755, 773, 899, 1469, 1509, 2156, 2180
AflIII ACRYGT 2 cut(s) 2134, 2257
AgeI ACCGGT 1 cut(s) 494
AgsI TTSAA 9 cut(s) 124, 250, 709, 1221, 1268, 1293, 1412, 1846, 2696
AhdI GACNNNNNGTC 2 cut(s) 573, 883
AjiI CACGTC 1 cut(s) 691
AjnI CCWGG 5 cut(s) 180, 344, 1044, 1185, 2101
Alw21I GWGCWC 5 cut(s) 414, 767, 1074, 2232, 2428
Alw26I GTCTC 3 cut(s) 290, 1367, 2323
Alw44I GTGCAC 1 cut(s) 763
AlwI GGATC 7 cut(s) 62, 349, 1140, 1328, 1399, 1823, 1889
Ama87I CYCGRG 2 cut(s) 1756, 1874
ApaI GGGCCC 1 cut(s) 652
ApaLI GTGCAC 1 cut(s) 763
ApeKI GCWGC 3 cut(s) 441, 611, 2005
ApoI RAATTY 3 cut(s) 1780, 2264, 2488
AsiGI ACCGGT 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 2653
Asp718I GGTACC 2 cut(s) 897, 2154
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 3 cut(s) 233, 311, 327
AsuC2I CCSGG 4 cut(s) 72, 416, 619, 1622
AvaI CYCGRG 2 cut(s) 1756, 1874
AvaII GGWCC 5 cut(s) 376, 492, 1040, 1106, 1941
AvrII CCTAGG 1 cut(s) 31
AxyI CCTNAGG 3 cut(s) 329, 579, 2318
BaeGI GKGCMC 4 cut(s) 652, 767, 803, 1441
BalI TGGCCA 1 cut(s) 1062
BanI GGYRCC 5 cut(s) 230, 897, 1276, 1438, 2154
BanII GRGCYC 4 cut(s) 652, 1074, 2232, 2642
Bbv12I GWGCWC 5 cut(s) 414, 767, 1074, 2232, 2428
BbvI GCAGC 3 cut(s) 453, 598, 1992
BccI CCATC 8 cut(s) 32, 558, 1494, 1514, 1736, 1858, 2141, 2474
BciT130I CCWGG 5 cut(s) 182, 346, 1046, 1187, 2103
BciVI GTATCC 1 cut(s) 994
BclI TGATCA 3 cut(s) 1564, 1948, 1960
BcnI CCSGG 4 cut(s) 72, 416, 619, 1622
BcoDI GTCTC 3 cut(s) 290, 1367, 2323
BfaI CTAG 3 cut(s) 32, 1463, 2121
BfmI CTRYAG 2 cut(s) 1110, 2022
BfoI RGCGCY 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 1791
BfuI GTATCC 1 cut(s) 994
BglI GCCNNNNNGGC 1 cut(s) 991
BglII AGATCT 1 cut(s) 2399
BisI GCNGC 5 cut(s) 442, 612, 2006, 2118, 2467
BlnI CCTAGG 1 cut(s) 31
BlpI GCTNAGC 1 cut(s) 2226
BlsI GCNGC 5 cut(s) 443, 613, 2007, 2119, 2468
BmcAI AGTACT 1 cut(s) 2180
Bme1390I CCNGG 9 cut(s) 72, 182, 346, 416, 619, 1046, 1187, 1622, 2103
Bme18I GGWCC 5 cut(s) 376, 492, 1040, 1106, 1941
BmeRI GACNNNNNGTC 2 cut(s) 573, 883
BmeT110I CYCGRG 2 cut(s) 1756, 1874
BmgBI CACGTC 1 cut(s) 691
BmrFI CCNGG 9 cut(s) 72, 182, 346, 416, 619, 1046, 1187, 1622, 2103
BmsI GCATC 3 cut(s) 1144, 1741, 1774
BoxI GACNNNNGTC 1 cut(s) 399
BplI GAGNNNNNCTC 2 cut(s) 2606, 2638
BpmI CTGGAG 3 cut(s) 116, 626, 1407
Bpu1102I GCTNAGC 1 cut(s) 2226
BpuEI CTTGAG 2 cut(s) 1386, 2630
BpuMI CCSGG 4 cut(s) 72, 416, 619, 1622
BsaAI YACGTR 1 cut(s) 1792
BsaBI GATNNNNATC 1 cut(s) 1950
BsaHI GRCGYC 3 cut(s) 216, 231, 879
BsaI GGTCTC 1 cut(s) 2323
BsaJI CCNNGG 7 cut(s) 31, 978, 1033, 1044, 1422, 1621, 2233
BsaWI WCCGGW 5 cut(s) 494, 870, 997, 1252, 2157
BsaXI ACNNNNNCTCC 8 cut(s) 158, 188, 188, 218, 675, 705, 2334, 2364
Bse118I RCCGGY 4 cut(s) 263, 469, 494, 1279
Bse1I ACTGG 8 cut(s) 227, 670, 1416, 1596, 1739, 2048, 2118, 2303
Bse21I CCTNAGG 3 cut(s) 329, 579, 2318
Bse3DI GCAATG 1 cut(s) 1932
Bse8I GATNNNNATC 1 cut(s) 1950
BseBI CCWGG 5 cut(s) 182, 346, 1046, 1187, 2103
BseDI CCNNGG 7 cut(s) 31, 978, 1033, 1044, 1422, 1621, 2233
BseJI GATNNNNATC 1 cut(s) 1950
BseMI GCAATG 1 cut(s) 1932
BseMII CTCAG 8 cut(s) 157, 320, 501, 570, 1074, 1349, 2196, 2217
BseNI ACTGG 8 cut(s) 227, 670, 1416, 1596, 1739, 2048, 2118, 2303
BseRI GAGGAG 6 cut(s) 32, 35, 379, 596, 1896, 2627
BseSI GKGCMC 4 cut(s) 652, 767, 803, 1441
BseXI GCAGC 3 cut(s) 453, 598, 1992
BseYI CCCAGC 2 cut(s) 1301, 2599
BsgI GTGCAG 1 cut(s) 1819
Bsh1236I CGCG 1 cut(s) 311
BshNI GGYRCC 5 cut(s) 230, 897, 1276, 1438, 2154
BshTI ACCGGT 1 cut(s) 494
BsiHKAI GWGCWC 5 cut(s) 414, 767, 1074, 2232, 2428
BsiHKCI CYCGRG 2 cut(s) 1756, 1874
BslFI GGGAC 7 cut(s) 347, 389, 505, 835, 890, 1053, 1404
BsmAI GTCTC 3 cut(s) 290, 1367, 2323
BsmFI GGGAC 7 cut(s) 347, 389, 505, 835, 890, 1053, 1404
BsmI GAATGC 4 cut(s) 726, 1354, 1369, 2314
Bso31I GGTCTC 1 cut(s) 2323
BsoBI CYCGRG 2 cut(s) 1756, 1874
Bsp120I GGGCCC 1 cut(s) 648
Bsp1286I GDGCHC 9 cut(s) 414, 652, 767, 803, 1074, 1441, 2232, 2428, 2642
Bsp1407I TGTACA 1 cut(s) 771
Bsp1720I GCTNAGC 1 cut(s) 2226
Bsp19I CCATGG 1 cut(s) 1033
BspACI CCGC 9 cut(s) 18, 261, 652, 941, 1151, 1166, 1720, 2117, 2466
BspCNI CTCAG 8 cut(s) 156, 321, 502, 571, 1075, 1350, 2195, 2218
BspFNI CGCG 1 cut(s) 311
BspMAI CTGCAG 1 cut(s) 2026
BspMI ACCTGC 1 cut(s) 1791
BspPI GGATC 7 cut(s) 62, 349, 1140, 1328, 1399, 1823, 1889
BspT107I GGYRCC 5 cut(s) 230, 897, 1276, 1438, 2154
BspTNI GGTCTC 1 cut(s) 2323
BsrDI GCAATG 1 cut(s) 1932
BsrFI RCCGGY 4 cut(s) 263, 469, 494, 1279
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 8 cut(s) 227, 670, 1416, 1596, 1739, 2048, 2118, 2303
BssAI RCCGGY 4 cut(s) 263, 469, 494, 1279
BssECI CCNNGG 7 cut(s) 31, 978, 1033, 1044, 1422, 1621, 2233
BssNAI GTATAC 1 cut(s) 2170
BssNI GRCGYC 3 cut(s) 216, 231, 879
BssT1I CCWWGG 3 cut(s) 31, 1033, 1422
Bst1107I GTATAC 1 cut(s) 2170
Bst2UI CCWGG 5 cut(s) 182, 346, 1046, 1187, 2103
Bst4CI ACNGT 6 cut(s) 322, 769, 967, 1100, 1378, 1550
Bst6I CTCTTC 1 cut(s) 1011
BstACI GRCGYC 3 cut(s) 216, 231, 879
BstAPI GCANNNNNTGC 1 cut(s) 1931
BstAUI TGTACA 1 cut(s) 771
BstBAI YACGTR 1 cut(s) 1792
BstC8I GCNNGC 7 cut(s) 11, 265, 313, 471, 475, 652, 1369
BstDSI CCRYGG 3 cut(s) 978, 1033, 2233
BstENI CCTNNNNNAGG 2 cut(s) 2359, 2612
BstFNI CGCG 1 cut(s) 311
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 3 cut(s) 233, 311, 327
BstMAI GTCTC 3 cut(s) 290, 1367, 2323
BstMWI GCNNNNNNNGC 5 cut(s) 10, 692, 991, 1931, 2011
BstNI CCWGG 5 cut(s) 182, 346, 1046, 1187, 2103
BstNSI RCATGY 5 cut(s) 202, 1091, 1972, 2138, 2261
BstPAI GACNNNNGTC 1 cut(s) 399
BstSCI CCNGG 9 cut(s) 70, 180, 344, 414, 617, 1044, 1185, 1620, 2101
BstSFI CTRYAG 2 cut(s) 1110, 2022
BstSLI GKGCMC 4 cut(s) 652, 767, 803, 1441
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 3 cut(s) 453, 598, 1992
BstX2I RGATCY 3 cut(s) 354, 1333, 2399
BstXI CCANNNNNNTGG 1 cut(s) 1034
BstYI RGATCY 3 cut(s) 354, 1333, 2399
BstZ17I GTATAC 1 cut(s) 2170
Bsu36I CCTNAGG 3 cut(s) 329, 579, 2318
BsuI GTATCC 1 cut(s) 994
BtgI CCRYGG 3 cut(s) 978, 1033, 2233
BtrI CACGTC 1 cut(s) 691
BtsI GCAGTG 3 cut(s) 569, 1197, 2064
BveI ACCTGC 1 cut(s) 1791
Cac8I GCNNGC 7 cut(s) 11, 265, 313, 471, 475, 652, 1369
CfoI GCGC 3 cut(s) 233, 311, 327
Cfr10I RCCGGY 4 cut(s) 263, 469, 494, 1279
CseI GACGC 2 cut(s) 563, 887
Csp6I GTAC 7 cut(s) 754, 772, 898, 1468, 1508, 2155, 2179
CspAI ACCGGT 1 cut(s) 494
CviQI GTAC 7 cut(s) 754, 772, 898, 1468, 1508, 2155, 2179
DinI GGCGCC 1 cut(s) 232
DraIII CACNNNGTG 1 cut(s) 409
DrdI GACNNNNNNGTC 2 cut(s) 695, 785
DriI GACNNNNNGTC 2 cut(s) 573, 883
DseDI GACNNNNNNGTC 2 cut(s) 695, 785
EaeI YGGCCR 4 cut(s) 693, 865, 1060, 1183
Eam1104I CTCTTC 1 cut(s) 1011
Eam1105I GACNNNNNGTC 2 cut(s) 573, 883
EarI CTCTTC 1 cut(s) 1011
Ecl136II GAGCTC 2 cut(s) 1072, 2230
Eco130I CCWWGG 3 cut(s) 31, 1033, 1422
Eco147I AGGCCT 1 cut(s) 1993
Eco24I GRGCYC 4 cut(s) 652, 1074, 2232, 2642
Eco31I GGTCTC 1 cut(s) 2323
Eco47I GGWCC 5 cut(s) 376, 492, 1040, 1106, 1941
Eco53kI GAGCTC 2 cut(s) 1072, 2230
Eco57I CTGAAG 3 cut(s) 992, 1314, 2462
Eco81I CCTNAGG 3 cut(s) 329, 579, 2318
Eco88I CYCGRG 2 cut(s) 1756, 1874
EcoICRI GAGCTC 2 cut(s) 1072, 2230
EcoNI CCTNNNNNAGG 2 cut(s) 2359, 2612
EcoO109I RGGNCCY 3 cut(s) 376, 1040, 1162
EcoRII CCWGG 5 cut(s) 180, 344, 1044, 1185, 2101
EcoT14I CCWWGG 3 cut(s) 31, 1033, 1422
EcoT22I ATGCAT 2 cut(s) 1974, 2314
EcoT38I GRGCYC 4 cut(s) 652, 1074, 2232, 2642
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 3 cut(s) 31, 1033, 1422
FaqI GGGAC 7 cut(s) 347, 389, 505, 835, 890, 1053, 1404
FauI CCCGC 3 cut(s) 659, 1173, 1713
FbaI TGATCA 3 cut(s) 1564, 1948, 1960
FblI GTMKAC 2 cut(s) 623, 2169
Fnu4HI GCNGC 5 cut(s) 442, 612, 2006, 2118, 2467
FriOI GRGCYC 4 cut(s) 652, 1074, 2232, 2642
Fsp4HI GCNGC 5 cut(s) 442, 612, 2006, 2118, 2467
FspBI CTAG 3 cut(s) 32, 1463, 2121
GlaI GCGC 3 cut(s) 232, 310, 326
GluI GCNGC 5 cut(s) 442, 612, 2006, 2118, 2467
GsaI CCCAGC 2 cut(s) 1305, 2603
GsuI CTGGAG 3 cut(s) 116, 626, 1407
HaeII RGCGCY 1 cut(s) 234
HgaI GACGC 2 cut(s) 563, 887
HhaI GCGC 3 cut(s) 233, 311, 327
Hin1I GRCGYC 3 cut(s) 216, 231, 879
Hin6I GCGC 3 cut(s) 231, 309, 325
HinP1I GCGC 3 cut(s) 231, 309, 325
HincII GTYRAC 2 cut(s) 106, 624
HindII GTYRAC 2 cut(s) 106, 624
HinfI GANTC 9 cut(s) 113, 349, 479, 705, 914, 1601, 2161, 2621, 2666
Hpy188III TCNNGA 5 cut(s) 578, 1202, 2512, 2540, 2679
Hpy99I CGWCG 7 cut(s) 170, 218, 239, 469, 551, 628, 701
HpyAV CCTTC 7 cut(s) 187, 468, 812, 1227, 1498, 2219, 2556
HpyCH4III ACNGT 6 cut(s) 322, 769, 967, 1100, 1378, 1550
HpyCH4IV ACGT 5 cut(s) 216, 690, 1286, 1791, 1823
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 692, 991, 1931, 2011
HpySE526I ACGT 5 cut(s) 216, 690, 1286, 1791, 1823
Hsp92I GRCGYC 3 cut(s) 216, 231, 879
HspAI GCGC 3 cut(s) 231, 309, 325
KasI GGCGCC 1 cut(s) 230
KflI GGGWCCC 1 cut(s) 1040
KpnI GGTACC 2 cut(s) 901, 2158
KroI GCCGGC 2 cut(s) 263, 469
KroNI GCCGGC 2 cut(s) 265, 471
Ksp22I TGATCA 3 cut(s) 1564, 1948, 1960
Lsp1109I GCAGC 3 cut(s) 453, 598, 1992
LweI GCATC 3 cut(s) 1144, 1741, 1774
MaeI CTAG 3 cut(s) 32, 1463, 2121
MaeII ACGT 5 cut(s) 216, 690, 1286, 1791, 1823
MaeIII GTNAC 5 cut(s) 340, 401, 1174, 1282, 1573
MboII GAAGA 6 cut(s) 19, 998, 1013, 1642, 2372, 2694
MfeI CAATTG 2 cut(s) 79, 2590
MflI RGATCY 3 cut(s) 354, 1333, 2399
MhlI GDGCHC 9 cut(s) 414, 652, 767, 803, 1074, 1441, 2232, 2428, 2642
MlsI TGGCCA 1 cut(s) 1062
MluNI TGGCCA 1 cut(s) 1062
Mly113I GGCGCC 1 cut(s) 231
MlyI GAGTC 1 cut(s) 2615
MmeI TCCRAC 8 cut(s) 237, 303, 417, 570, 891, 1676, 1793, 1922
Mox20I TGGCCA 1 cut(s) 1062
Mph1103I ATGCAT 2 cut(s) 1974, 2314
MreI CGCCGGCG 1 cut(s) 469
MroNI GCCGGC 2 cut(s) 263, 469
MroXI GAANNNNTTC 1 cut(s) 2653
MscI TGGCCA 1 cut(s) 1062
MseI TTAA 7 cut(s) 156, 1236, 1430, 1911, 2129, 2139, 2262
MslI CAYNNNNRTG 3 cut(s) 1092, 1956, 2451
Msp20I TGGCCA 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 920
MspR9I CCNGG 9 cut(s) 72, 182, 346, 416, 619, 1046, 1187, 1622, 2103
MunI CAATTG 2 cut(s) 79, 2590
Mva1269I GAATGC 4 cut(s) 726, 1354, 1369, 2314
MvaI CCWGG 5 cut(s) 182, 346, 1046, 1187, 2103
MvnI CGCG 1 cut(s) 311
MwoI GCNNNNNNNGC 5 cut(s) 10, 692, 991, 1931, 2011
NaeI GCCGGC 2 cut(s) 265, 471
NarI GGCGCC 1 cut(s) 231
NciI CCSGG 4 cut(s) 72, 416, 619, 1622
NcoI CCATGG 1 cut(s) 1033
NgoMIV GCCGGC 2 cut(s) 263, 469
NmuCI GTSAC 3 cut(s) 340, 401, 1282
NsiI ATGCAT 2 cut(s) 1974, 2314
NspI RCATGY 5 cut(s) 202, 1091, 1972, 2138, 2261
PaeR7I CTCGAG 1 cut(s) 1756
PceI AGGCCT 1 cut(s) 1993
PciI ACATGT 2 cut(s) 2134, 2257
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 405, 687
PctI GAATGC 4 cut(s) 726, 1354, 1369, 2314
PdiI GCCGGC 2 cut(s) 265, 471
PdmI GAANNNNTTC 1 cut(s) 2653
PfeI GAWTC 8 cut(s) 113, 349, 479, 705, 914, 1601, 2161, 2666
PflFI GACNNNGTC 1 cut(s) 338
PflMI CCANNNNNTGG 4 cut(s) 1051, 1495, 1856, 2339
PfoI TCCNGGA 1 cut(s) 414
PinAI ACCGGT 1 cut(s) 494
PkrI GCNGC 5 cut(s) 443, 613, 2007, 2119, 2468
PleI GAGTC 1 cut(s) 2615
PluTI GGCGCC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 2615
Ppu21I YACGTR 1 cut(s) 1792
PpuMI RGGWCCY 2 cut(s) 376, 1040
PscI ACATGT 2 cut(s) 2134, 2257
PshAI GACNNNNGTC 1 cut(s) 399
Psp124BI GAGCTC 2 cut(s) 1074, 2232
Psp5II RGGWCCY 2 cut(s) 376, 1040
Psp6I CCWGG 5 cut(s) 180, 344, 1044, 1185, 2101
PspFI CCCAGC 2 cut(s) 1301, 2599
PspGI CCWGG 5 cut(s) 180, 344, 1044, 1185, 2101
PspOMI GGGCCC 1 cut(s) 648
PspPPI RGGWCCY 2 cut(s) 376, 1040
PspXI VCTCGAGB 1 cut(s) 1756
PstI CTGCAG 1 cut(s) 2026
PsuI RGATCY 3 cut(s) 354, 1333, 2399
PsyI GACNNNGTC 1 cut(s) 338
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 7 cut(s) 755, 773, 899, 1469, 1509, 2156, 2180
RsaNI GTAC 7 cut(s) 754, 772, 898, 1468, 1508, 2155, 2179
RseI CAYNNNNRTG 3 cut(s) 1092, 1956, 2451
SacI GAGCTC 2 cut(s) 1074, 2232
SalI GTCGAC 1 cut(s) 622
SaqAI TTAA 7 cut(s) 156, 1236, 1430, 1911, 2129, 2139, 2262
SatI GCNGC 5 cut(s) 442, 612, 2006, 2118, 2467
ScaI AGTACT 1 cut(s) 2180
SchI GAGTC 1 cut(s) 2615
ScrFI CCNGG 9 cut(s) 72, 182, 346, 416, 619, 1046, 1187, 1622, 2103
SduI GDGCHC 9 cut(s) 414, 652, 767, 803, 1074, 1441, 2232, 2428, 2642
SfaNI GCATC 3 cut(s) 1144, 1741, 1774
SfcI CTRYAG 2 cut(s) 1110, 2022
SfiI GGCCNNNNNGGCC 1 cut(s) 991
SfoI GGCGCC 1 cut(s) 232
Sfr274I CTCGAG 1 cut(s) 1756
SgrAI CRCCGGYG 1 cut(s) 469
SinI GGWCC 5 cut(s) 376, 492, 1040, 1106, 1941
SlaI CTCGAG 1 cut(s) 1756
SmiMI CAYNNNNRTG 3 cut(s) 1092, 1956, 2451
SmlI CTYRAG 3 cut(s) 1401, 1756, 2609
SmoI CTYRAG 3 cut(s) 1401, 1756, 2609
SseBI AGGCCT 1 cut(s) 1993
SsiI CCGC 9 cut(s) 18, 261, 652, 941, 1151, 1166, 1720, 2117, 2466
SspDI GGCGCC 1 cut(s) 230
SspI AATATT 1 cut(s) 1708
SspMI CTAG 3 cut(s) 32, 1463, 2121
SstI GAGCTC 2 cut(s) 1074, 2232
StuI AGGCCT 1 cut(s) 1993
StyD4I CCNGG 9 cut(s) 70, 180, 344, 414, 617, 1044, 1185, 1620, 2101
StyI CCWWGG 3 cut(s) 31, 1033, 1422
TaaI ACNGT 6 cut(s) 322, 769, 967, 1100, 1378, 1550
TaiI ACGT 5 cut(s) 219, 693, 1289, 1794, 1826
TaqI TCGA 5 cut(s) 165, 195, 623, 1757, 1827
TaqII GACCGA 2 cut(s) 389, 1958
TatI WGTACW 4 cut(s) 753, 771, 1467, 2178
TauI GCSGC 2 cut(s) 2120, 2469
TfiI GAWTC 8 cut(s) 113, 349, 479, 705, 914, 1601, 2161, 2666
Tru1I TTAA 7 cut(s) 156, 1236, 1430, 1911, 2129, 2139, 2262
Tru9I TTAA 7 cut(s) 156, 1236, 1430, 1911, 2129, 2139, 2262
TseFI GTSAC 3 cut(s) 340, 401, 1282
TseI GCWGC 3 cut(s) 441, 611, 2005
Tsp45I GTSAC 3 cut(s) 340, 401, 1282
TspDTI ATGAA 3 cut(s) 2153, 2372, 2565
TspGWI ACGGA 5 cut(s) 142, 453, 732, 775, 2222
Tth111I GACNNNGTC 1 cut(s) 338
Van91I CCANNNNNTGG 4 cut(s) 1051, 1495, 1856, 2339
VneI GTGCAC 1 cut(s) 763
VpaK11BI GGWCC 5 cut(s) 376, 492, 1040, 1106, 1941
XagI CCTNNNNNAGG 2 cut(s) 2359, 2612
XapI RAATTY 3 cut(s) 1780, 2264, 2488
XceI RCATGY 5 cut(s) 202, 1091, 1972, 2138, 2261
XhoI CTCGAG 1 cut(s) 1756
XmaJI CCTAGG 1 cut(s) 31
XmiI GTMKAC 2 cut(s) 623, 2169
XmnI GAANNNNTTC 1 cut(s) 2653
XspI CTAG 3 cut(s) 32, 1463, 2121
ZraI GACGTC 1 cut(s) 217
ZrmI AGTACT 1 cut(s) 2180
Zsp2I ATGCAT 2 cut(s) 1974, 2314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.