pycom07g00270

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005345
Physical Location & Seq
Forward (+)
279502 .. 283388
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGATCTTTGTTGATGGGCTTCCCTTTTCTACAGACTCTTCTGCATCGCCTCAGGGATGGGATACTCTGGTTGCTCTACTGGAACACCCTGCTTTGGTAGAAGCCTCAAATTCGTTCAAAGCTATCTCGGAGAGGAGATTCTCTGTACGTGAAGAATCAGTTCTGGACACATCGCCACGTAGTAAATGGGTTTATTTGTTCCAGAGAGAATATGCAACTGTTGATCCAGCATTGGTGGATTTTGTTGGAACTGATGAAGCAACAACCTGTATCGGTGTGGCCATCCGGAATCCTAGAAATGGAATGACCTCAGTTGCTCATATGGATTCTCCAAACATTGTGGACACGGGTTTATCCCAAATGCTGTCACTCCTTGTTGACCATAACACGGATATGGAGTTAGATGTGCATCTAGTTGGTGGATTTGAAGATGTTTCAACTAATCATGGTAACTACAACACTGGATCAGAAAGTCAGGAAAACTTGGATGGTTATTCCTTCCCTTTAGGTGCCAAAATTGTTGAAACTTTAAGGAAGAGACCAGAGAAATTCCATATACGTACTCTATGCATTCTTGGGCATAACACTAGAAGGGATCCCGAGGGAAACGCGTACCCAAGCTTCAACGGTTTCATGATAGGAACTTCCACCGGATCTATTACCCCAGCTATCTTTGACAAAACCTCAAGATGCCCTGATGAAGTTGTCAGGAGAATTCGAGTTTCTGTATCATATGAGGACACTAGTTGGAAAGGAAAGTTACTAGAAACATATGATACCCAAACTGATCATTTCAAGATAGCTCCATGTTGCTGGACACTGCAGCAGTTACATTTTGCTTTGTCATTACAACGCTACTCTGATTCAGAAATCCTTCTCATGTGTTCTACTTCACCTTCTGCTGAGAACCCAGATTTTGTGGAGAATATGAGAAGGCAATGGGACTACCTAATTAAACACCCAGATTGGAGAGAAACCTTTCCCATGAAGCAGCCACGCATATTTGAGAGGACTGCTGAAGGTGGCTGGAGAAAGCCACAAGGTGCTGATACATTAATGAATAGGAGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

358

Amino Acids

40.46

Weight (kDa)

5.43

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 59 - 344 1.1e-84 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AccII CGCG 1 cut(s) 611
AccIII TCCGGA 1 cut(s) 285
AclWI GGATC 5 cut(s) 218, 472, 590, 603, 661
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 109, 548, 714
AcuI CTGAAG 1 cut(s) 1038
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 3 cut(s) 147, 562, 614
AfiI CCNNNNNNNGG 3 cut(s) 94, 299, 388
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 6 cut(s) 118, 428, 438, 524, 625, 796
AhlI ACTAGT 1 cut(s) 743
AluBI AGCT 4 cut(s) 122, 621, 668, 803
AluI AGCT 4 cut(s) 122, 621, 668, 803
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 5 cut(s) 218, 472, 590, 603, 661
Ama87I CYCGRG 1 cut(s) 599
Aor13HI TCCGGA 1 cut(s) 285
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 823, 991
ApoI RAATTY 3 cut(s) 109, 548, 714
AseI ATTAAT 1 cut(s) 1055
Asp700I GAANNNNTTC 3 cut(s) 159, 873, 978
AsuHPI GGTGA 1 cut(s) 885
AvaI CYCGRG 1 cut(s) 599
AxyI CCTNAGG 1 cut(s) 51
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BbvI GCAGC 2 cut(s) 835, 1003
BccI CCATC 4 cut(s) 8, 51, 290, 482
BciVI GTATCC 1 cut(s) 55
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 1 cut(s) 532
BcuI ACTAGT 1 cut(s) 743
BfaI CTAG 6 cut(s) 294, 413, 588, 744, 764, 1072
BfmI CTRYAG 2 cut(s) 30, 821
BfuI GTATCC 1 cut(s) 55
BisI GCNGC 2 cut(s) 824, 992
BlsI GCNGC 2 cut(s) 825, 993
BmeT110I CYCGRG 1 cut(s) 599
BmiI GGNNCC 2 cut(s) 511, 597
BmsI GCATC 3 cut(s) 53, 418, 680
BpmI CTGGAG 1 cut(s) 1048
BpuEI CTTGAG 1 cut(s) 670
BsaAI YACGTR 3 cut(s) 149, 179, 560
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 1 cut(s) 600
BsaWI WCCGGW 2 cut(s) 285, 650
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 299, 388
Bse1I ACTGG 2 cut(s) 84, 466
Bse21I CCTNAGG 1 cut(s) 51
Bse3DI GCAATG 1 cut(s) 944
Bse8I GATNNNNATC 1 cut(s) 408
BseAI TCCGGA 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 600
BseGI GGATG 3 cut(s) 62, 282, 493
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 94, 299, 388
BseMI GCAATG 1 cut(s) 944
BseMII CTCAG 3 cut(s) 65, 324, 894
BseNI ACTGG 2 cut(s) 84, 466
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 2 cut(s) 835, 1003
BseYI CCCAGC 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 611
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 509
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 2 cut(s) 286, 651
BslFI GGGAC 1 cut(s) 956
BslI CCNNNNNNNGG 3 cut(s) 94, 299, 388
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 956
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 532
BsoBI CYCGRG 1 cut(s) 599
Bsp13I TCCGGA 1 cut(s) 285
Bsp143I GATC 6 cut(s) 3, 223, 464, 595, 653, 787
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 64, 323, 895
BspEI TCCGGA 1 cut(s) 285
BspFNI CGCG 1 cut(s) 611
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 2 cut(s) 511, 597
BspMAI CTGCAG 1 cut(s) 825
BspPI GGATC 5 cut(s) 218, 472, 590, 603, 661
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 944
BsrI ACTGG 2 cut(s) 84, 466
BssECI CCNNGG 1 cut(s) 600
BssMI GATC 6 cut(s) 3, 223, 464, 595, 653, 787
Bst4CI ACNGT 2 cut(s) 220, 629
Bst6I CTCTTC 2 cut(s) 43, 530
BstBAI YACGTR 3 cut(s) 149, 179, 560
BstDEI CTNAG 3 cut(s) 51, 310, 903
BstF5I GGATG 3 cut(s) 62, 282, 493
BstFNI CGCG 1 cut(s) 611
BstKTI GATC 6 cut(s) 6, 226, 467, 598, 656, 790
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 6 cut(s) 3, 223, 464, 595, 653, 787
BstSFI CTRYAG 2 cut(s) 30, 821
BstSNI TACGTA 1 cut(s) 560
BstUI CGCG 1 cut(s) 611
BstV1I GCAGC 2 cut(s) 835, 1003
BstX2I RGATCY 2 cut(s) 595, 653
BstXI CCANNNNNNTGG 1 cut(s) 813
BstYI RGATCY 2 cut(s) 595, 653
Bsu36I CCTNAGG 1 cut(s) 51
BsuI GTATCC 1 cut(s) 55
BsuRI GGCC 1 cut(s) 281
BtgZI GCGATG 2 cut(s) 30, 156
BtsCI GGATG 3 cut(s) 62, 282, 493
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 2 cut(s) 459, 818
CciI TCATGA 1 cut(s) 633
Csp6I GTAC 3 cut(s) 146, 561, 613
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 5 cut(s) 446, 634, 807, 880, 985
CviQI GTAC 3 cut(s) 146, 561, 613
DdeI CTNAG 3 cut(s) 51, 310, 903
DpnI GATC 6 cut(s) 5, 225, 466, 597, 655, 789
DpnII GATC 6 cut(s) 3, 223, 464, 595, 653, 787
DraIII CACNNNGTG 1 cut(s) 1043
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 43, 530
EarI CTCTTC 2 cut(s) 43, 530
Eco105I TACGTA 1 cut(s) 560
Eco31I GGTCTC 1 cut(s) 532
Eco57I CTGAAG 1 cut(s) 1038
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 599
EcoRI GAATTC 1 cut(s) 714
EcoT22I ATGCAT 1 cut(s) 572
FaeI CATG 5 cut(s) 449, 637, 810, 883, 988
FaqI GGGAC 1 cut(s) 956
FatI CATG 5 cut(s) 445, 633, 806, 879, 984
FauNDI CATATG 3 cut(s) 321, 733, 772
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 824, 992
FokI GGATG 3 cut(s) 69, 269, 500
Fsp4HI GCNGC 2 cut(s) 824, 992
FspBI CTAG 6 cut(s) 294, 413, 588, 744, 764, 1072
GluI GCNGC 2 cut(s) 824, 992
GsaI CCCAGC 1 cut(s) 668
GsuI CTGGAG 1 cut(s) 1048
HaeIII GGCC 1 cut(s) 281
HapII CCGG 2 cut(s) 286, 651
Hin1II CATG 5 cut(s) 449, 637, 810, 883, 988
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 6 cut(s) 35, 138, 155, 289, 326, 863
HpaII CCGG 2 cut(s) 286, 651
HphI GGTGA 1 cut(s) 885
Hpy166II GTNNAC 2 cut(s) 343, 379
Hpy188I TCNGA 4 cut(s) 130, 469, 862, 868
Hpy188III TCNNGA 9 cut(s) 164, 202, 286, 476, 599, 634, 687, 709, 796
Hpy8I GTNNAC 2 cut(s) 343, 379
HpyAV CCTTC 6 cut(s) 508, 585, 884, 906, 927, 1013
HpyCH4III ACNGT 2 cut(s) 220, 629
HpyCH4IV ACGT 3 cut(s) 148, 178, 559
HpyCH4V TGCA 5 cut(s) 44, 215, 409, 570, 823
HpyF3I CTNAG 3 cut(s) 51, 310, 903
HpySE526I ACGT 3 cut(s) 148, 178, 559
Hsp92II CATG 5 cut(s) 449, 637, 810, 883, 988
Kpn2I TCCGGA 1 cut(s) 285
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 6 cut(s) 3, 223, 464, 595, 653, 787
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 2 cut(s) 835, 1003
LweI GCATC 3 cut(s) 53, 418, 680
MaeI CTAG 6 cut(s) 294, 413, 588, 744, 764, 1072
MaeII ACGT 3 cut(s) 148, 178, 559
MaeIII GTNAC 4 cut(s) 366, 449, 759, 828
MalI GATC 6 cut(s) 5, 225, 466, 597, 655, 789
MboI GATC 6 cut(s) 3, 223, 464, 595, 653, 787
MboII GAAGA 4 cut(s) 30, 164, 440, 547
MflI RGATCY 2 cut(s) 595, 653
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 5 cut(s) 109, 516, 548, 714, 951
MluI ACGCGT 1 cut(s) 609
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 2 cut(s) 226, 728
MnlI CCTC 9 cut(s) 60, 115, 126, 319, 595, 694, 730, 1002, 1059
Mox20I TGGCCA 1 cut(s) 281
Mph1103I ATGCAT 1 cut(s) 572
MroI TCCGGA 1 cut(s) 285
MroXI GAANNNNTTC 3 cut(s) 159, 873, 978
MscI TGGCCA 1 cut(s) 281
MseI TTAA 3 cut(s) 530, 954, 1055
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 2 cut(s) 286, 651
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 611
NdeI CATATG 3 cut(s) 321, 733, 772
NdeII GATC 6 cut(s) 3, 223, 464, 595, 653, 787
NlaIII CATG 5 cut(s) 449, 637, 810, 883, 988
NlaIV GGNNCC 2 cut(s) 511, 597
NmuCI GTSAC 1 cut(s) 366
NsiI ATGCAT 1 cut(s) 572
PagI TCATGA 1 cut(s) 633
PctI GAATGC 1 cut(s) 570
PdmI GAANNNNTTC 3 cut(s) 159, 873, 978
PfeI GAWTC 5 cut(s) 138, 155, 289, 326, 863
PkrI GCNGC 2 cut(s) 825, 993
PleI GAGTC 1 cut(s) 29
PpsI GAGTC 1 cut(s) 29
Ppu21I YACGTR 3 cut(s) 149, 179, 560
PshBI ATTAAT 1 cut(s) 1055
PspFI CCCAGC 1 cut(s) 664
PspN4I GGNNCC 2 cut(s) 511, 597
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 2 cut(s) 595, 653
RsaI GTAC 3 cut(s) 147, 562, 614
RsaNI GTAC 3 cut(s) 146, 561, 613
RseI CAYNNNNRTG 1 cut(s) 392
SaqAI TTAA 3 cut(s) 530, 954, 1055
SatI GCNGC 2 cut(s) 824, 992
Sau3AI GATC 6 cut(s) 3, 223, 464, 595, 653, 787
SchI GAGTC 1 cut(s) 29
SfaNI GCATC 3 cut(s) 53, 418, 680
SfcI CTRYAG 2 cut(s) 30, 821
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
SnaBI TACGTA 1 cut(s) 560
SpeI ACTAGT 1 cut(s) 743
Sse9I AATT 5 cut(s) 109, 516, 548, 714, 951
SspMI CTAG 6 cut(s) 294, 413, 588, 744, 764, 1072
TaaI ACNGT 2 cut(s) 220, 629
TaiI ACGT 3 cut(s) 151, 181, 562
TaqI TCGA 1 cut(s) 718
TasI AATT 5 cut(s) 109, 516, 548, 714, 951
TfiI GAWTC 5 cut(s) 138, 155, 289, 326, 863
Tru1I TTAA 3 cut(s) 530, 954, 1055
Tru9I TTAA 3 cut(s) 530, 954, 1055
TscAI CASTG 2 cut(s) 466, 825
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 2 cut(s) 823, 991
Tsp45I GTSAC 1 cut(s) 366
TspDTI ATGAA 5 cut(s) 270, 622, 714, 1001, 1073
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 2 cut(s) 466, 825
VspI ATTAAT 1 cut(s) 1055
XapI RAATTY 3 cut(s) 109, 548, 714
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XmnI GAANNNNTTC 3 cut(s) 159, 873, 978
XspI CTAG 6 cut(s) 294, 413, 588, 744, 764, 1072
Zsp2I ATGCAT 1 cut(s) 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.