pycom07g01050

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008425
Physical Location & Seq
Reverse (-)
865323 .. 871522
6200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1416 bp
ATGCTTTTTCGTTGGCAACCTTTCGTTTTCCAGTGGCTCTGTCTTCTGCCTCCGTCGACCAAGAATTGCCCTATCAAGCTATCAGCCTCCCTCCCGAGAGTCCATGGAACCAACACACACAACCAGCAGGAGCCTCTGAGATTCTCTGAACAGGGACTTCTTCAACAGCCACCGCATCCACGACCACCACTTTTAACTTCCTCTTCCTGTCAAACCCATCAAGTGCTCGGCAGTAGCACACCTAGCACACCTGCCGCTGCCGCCGTCATGGTTTATTCTCTCGCCACCCATCCTCACCACGCCCATTACCCTCCTCCTTGGTTTAGTATTGCTCCCATGATGGAGTGGACTGACAATCACTATAGGACGCTGGCCAGGCTCATTTCGAAGAATGCGTGGCTCTACACAGAGATGCTTGCTGCTGAGACCATTGTCTACCAGAAGGATAATCTGGATAGATTCTTGGAATATTCCCCGGAACAACATCCTATTGTTCTTCAAATTGGCGGGAATAATTTGGAAAACCTCGCCAAAGCGACTGAGCTTGCTAACCCTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCGAGTCCAAGAGTAGCTGGGCACGGGTGCTTTGGTGCTAGTCTTATGCTTGATCCAAAGTTTGTTGCTGAGGCTATGTCAGTGATTGCTGCGCACACAGATGCACCTGTTAGCGTGAAATGTCGAATTGGTGTTGATAATCATGATTCATATAACGAGCTCTGTGATTTTATTTACAAGGTTTCTTCTCAATCACCAACTAGGCATTTCATAATACATTCACGGAAGGCACTACTCAATGGAATTAGCCCAGCTGAAAATCGAAGTATTCCGCCTCTTAAATATGAGTACTTTTATGGCCTCTTGCGTGACTTTCCAGACTTGAGATTTACAATAAATGGGGGCATAAACACCGTTGAGGAGGTCAATGCAGCAAGAAGGGCAGGAGCTCATGGTGTAATGGTTGGACGTGCTGCATTTAATAAACCATGGCATACTTTGGGACATGTTGATACTGTAGTTTATGGAGCACCAAGCAGTGGTGTAACACGGCGTCAGATACTGGAGAAGTTTCAAGTGTATGGAGATACTGCTGTGGGAAAATATGGACGCAAACCAACTGTGCGAGATGTAGCAAGGCCTTTGATTGGTCTTTTCCATTCAGAACCTGGGAATAGTCAATGGAAACGCAAGGCTGATGCTGCTTTCCTGCATTGCACGACCATGAAAGAGTTTTTTGATGAAACGCTTGTAGCGATTCCTGACTATGTTCTGGATAAACCTATTGGGGAGCCACCATCAGGCAGTGAAGATCCTTTTGCGAACATACACAGTTTGCTGCCTCCGGCGTATGAATCAAGGGAACTAGAACTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

472

Amino Acids

52.67

Weight (kDa)

7.06

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 110 - 418 2.7e-59 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 259
Acc16I TGCGCA 1 cut(s) 684
Acc36I ACCTGC 1 cut(s) 259
AccB7I CCANNNNNTGG 1 cut(s) 1070
AccI GTMKAC 2 cut(s) 56, 435
AciI CCGC 5 cut(s) 173, 255, 261, 507, 863
AclWI GGATC 2 cut(s) 638, 1337
AcoI YGGCCR 1 cut(s) 372
AcyI GRCGYC 1 cut(s) 1084
AfaI GTAC 1 cut(s) 881
AfiI CCNNNNNNNGG 3 cut(s) 1070, 1178, 1331
AflIII ACRYGT 1 cut(s) 1036
AgsI TTSAA 3 cut(s) 164, 500, 1106
AjiI CACGTC 1 cut(s) 1001
AjnI CCWGG 2 cut(s) 374, 1198
AluBI AGCT 6 cut(s) 79, 544, 608, 751, 845, 980
AluI AGCT 6 cut(s) 79, 544, 608, 751, 845, 980
Alw21I GWGCWC 4 cut(s) 228, 753, 982, 1063
Alw26I GTCTC 1 cut(s) 419
AlwI GGATC 2 cut(s) 638, 1337
AlwNI CAGNNNCTG 2 cut(s) 1093, 1199
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 3 cut(s) 372, 889, 1169
ApeKI GCWGC 7 cut(s) 257, 419, 680, 962, 1004, 1232, 1369
AspLEI GCGC 1 cut(s) 685
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 2 cut(s) 287, 777
AsuII TTCGAA 1 cut(s) 386
AvaI CYCGRG 1 cut(s) 94
BaeGI GKGCMC 1 cut(s) 615
BaeI ACNNNNGTAYC 4 cut(s) 1035, 1068, 1110, 1143
BalI TGGCCA 1 cut(s) 374
BanII GRGCYC 2 cut(s) 753, 982
BbsI GAAGAC 1 cut(s) 35
Bbv12I GWGCWC 4 cut(s) 228, 753, 982, 1063
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 7 cut(s) 244, 406, 667, 974, 991, 1219, 1356
BccI CCATC 4 cut(s) 225, 297, 334, 1336
BceAI ACGGC 2 cut(s) 248, 1097
BciT130I CCWGG 2 cut(s) 376, 1200
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 4 cut(s) 243, 630, 792, 1397
BfmI CTRYAG 2 cut(s) 361, 1047
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 9 cut(s) 255, 258, 261, 420, 681, 963, 1005, 1233, 1370
BlsI GCNGC 9 cut(s) 256, 259, 262, 421, 682, 964, 1006, 1234, 1371
BmcAI AGTACT 1 cut(s) 881
Bme1390I CCNGG 3 cut(s) 376, 476, 1200
BmeT110I CYCGRG 1 cut(s) 94
BmgBI CACGTC 1 cut(s) 1001
BmiI GGNNCC 3 cut(s) 109, 132, 1323
BmrFI CCNGG 3 cut(s) 376, 476, 1200
BmsI GCATC 4 cut(s) 184, 402, 682, 1219
BoxI GACNNNNGTC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 35
BpmI CTGGAG 1 cut(s) 1115
Bpu10I CCTNAGC 1 cut(s) 660
Bpu14I TTCGAA 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 934
BpuMI CCSGG 1 cut(s) 476
BsaHI GRCGYC 1 cut(s) 1084
BsaI GGTCTC 1 cut(s) 419
BsaJI CCNNGG 5 cut(s) 103, 317, 474, 1019, 1199
Bsc4I CCNNNNNNNGG 3 cut(s) 1070, 1178, 1331
Bse1I ACTGG 2 cut(s) 31, 1098
Bse3DI GCAATG 1 cut(s) 1243
BseBI CCWGG 2 cut(s) 376, 1200
BseDI CCNNGG 5 cut(s) 103, 317, 474, 1019, 1199
BseGI GGATG 4 cut(s) 175, 289, 484, 593
BseLI CCNNNNNNNGG 3 cut(s) 1070, 1178, 1331
BseMI GCAATG 1 cut(s) 1243
BseMII CTCAG 4 cut(s) 128, 414, 531, 651
BseNI ACTGG 2 cut(s) 31, 1098
BseRI GAGGAG 2 cut(s) 303, 965
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 7 cut(s) 244, 406, 667, 974, 991, 1219, 1356
BseYI CCCAGC 2 cut(s) 608, 841
BshFI GGCC 3 cut(s) 374, 891, 1171
BsiHKAI GWGCWC 4 cut(s) 228, 753, 982, 1063
BsiHKCI CYCGRG 1 cut(s) 94
BsiSI CCGG 2 cut(s) 476, 1376
BslFI GGGAC 2 cut(s) 168, 1047
BslI CCNNNNNNNGG 3 cut(s) 1070, 1178, 1331
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 168, 1047
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 3 cut(s) 374, 891, 1171
Bso31I GGTCTC 1 cut(s) 419
BsoBI CYCGRG 1 cut(s) 94
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 5 cut(s) 228, 615, 753, 982, 1063
Bsp143I GATC 3 cut(s) 570, 643, 1342
Bsp19I CCATGG 2 cut(s) 103, 1019
BspACI CCGC 5 cut(s) 173, 255, 261, 507, 863
BspANI GGCC 3 cut(s) 374, 891, 1171
BspCNI CTCAG 4 cut(s) 129, 415, 532, 652
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 3 cut(s) 109, 132, 1323
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 2 cut(s) 638, 1337
BspT104I TTCGAA 1 cut(s) 386
BspTNI GGTCTC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 1243
BsrI ACTGG 2 cut(s) 31, 1098
BssECI CCNNGG 5 cut(s) 103, 317, 474, 1019, 1199
BssMI GATC 3 cut(s) 570, 643, 1342
BssNI GRCGYC 1 cut(s) 1084
BssT1I CCWWGG 3 cut(s) 103, 317, 1019
Bst2UI CCWGG 2 cut(s) 376, 1200
Bst4CI ACNGT 4 cut(s) 946, 1048, 1153, 1364
Bst6I CTCTTC 1 cut(s) 208
BstACI GRCGYC 1 cut(s) 1084
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 372, 417, 546
BstDEI CTNAG 5 cut(s) 137, 423, 540, 660, 1413
BstDSI CCRYGG 2 cut(s) 103, 1019
BstF5I GGATG 4 cut(s) 175, 289, 484, 593
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 3 cut(s) 573, 646, 1345
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 3 cut(s) 570, 643, 1342
BstMWI GCNNNNNNNGC 5 cut(s) 243, 260, 376, 971, 1232
BstNI CCWGG 2 cut(s) 376, 1200
BstNSI RCATGY 1 cut(s) 1040
BstPAI GACNNNNGTC 1 cut(s) 431
BstSCI CCNGG 3 cut(s) 374, 474, 1198
BstSFI CTRYAG 2 cut(s) 361, 1047
BstSLI GKGCMC 1 cut(s) 615
BstV1I GCAGC 7 cut(s) 244, 406, 667, 974, 991, 1219, 1356
BstV2I GAAGAC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 1342
BstYI RGATCY 1 cut(s) 1342
BsuRI GGCC 3 cut(s) 374, 891, 1171
BtgI CCRYGG 2 cut(s) 103, 1019
BtrI CACGTC 1 cut(s) 1001
BtsCI GGATG 4 cut(s) 175, 289, 484, 593
BtsI GCAGTG 2 cut(s) 1075, 1342
BtsIMutI CAGTG 4 cut(s) 38, 678, 1075, 1342
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 3 cut(s) 372, 417, 546
CaiI CAGNNNCTG 2 cut(s) 1093, 1199
CciI TCATGA 1 cut(s) 733
CfoI GCGC 1 cut(s) 685
CseI GACGC 3 cut(s) 376, 1073, 1149
Csp6I GTAC 1 cut(s) 880
CviAII CATG 8 cut(s) 104, 268, 337, 734, 983, 1020, 1037, 1255
CviQI GTAC 1 cut(s) 880
DdeI CTNAG 5 cut(s) 137, 423, 540, 660, 1413
DpnI GATC 3 cut(s) 572, 645, 1344
DpnII GATC 3 cut(s) 570, 643, 1342
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 208
EarI CTCTTC 1 cut(s) 208
EciI GGCGGA 1 cut(s) 852
Ecl136II GAGCTC 2 cut(s) 751, 980
Eco130I CCWWGG 3 cut(s) 103, 317, 1019
Eco147I AGGCCT 1 cut(s) 1171
Eco24I GRGCYC 2 cut(s) 753, 982
Eco31I GGTCTC 1 cut(s) 419
Eco53kI GAGCTC 2 cut(s) 751, 980
Eco88I CYCGRG 1 cut(s) 94
EcoICRI GAGCTC 2 cut(s) 751, 980
EcoRII CCWGG 2 cut(s) 374, 1198
EcoT14I CCWWGG 3 cut(s) 103, 317, 1019
EcoT38I GRGCYC 2 cut(s) 753, 982
ErhI CCWWGG 3 cut(s) 103, 317, 1019
FaeI CATG 8 cut(s) 107, 271, 340, 737, 986, 1023, 1040, 1258
FaqI GGGAC 2 cut(s) 168, 1047
FatI CATG 8 cut(s) 103, 267, 336, 733, 982, 1019, 1036, 1254
FauI CCCGC 1 cut(s) 500
FblI GTMKAC 2 cut(s) 56, 435
Fnu4HI GCNGC 9 cut(s) 255, 258, 261, 420, 681, 963, 1005, 1233, 1370
FokI GGATG 4 cut(s) 162, 276, 471, 600
FriOI GRGCYC 2 cut(s) 753, 982
Fsp4HI GCNGC 9 cut(s) 255, 258, 261, 420, 681, 963, 1005, 1233, 1370
FspBI CTAG 4 cut(s) 243, 630, 792, 1397
FspI TGCGCA 1 cut(s) 684
GlaI GCGC 1 cut(s) 684
GluI GCNGC 9 cut(s) 255, 258, 261, 420, 681, 963, 1005, 1233, 1370
GsaI CCCAGC 2 cut(s) 612, 845
GsuI CTGGAG 1 cut(s) 1115
HaeIII GGCC 3 cut(s) 374, 891, 1171
HapII CCGG 2 cut(s) 476, 1376
HgaI GACGC 3 cut(s) 376, 1073, 1149
HhaI GCGC 1 cut(s) 685
Hin1I GRCGYC 1 cut(s) 1084
Hin1II CATG 8 cut(s) 107, 271, 340, 737, 986, 1023, 1040, 1258
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 7 cut(s) 99, 141, 459, 595, 737, 1288, 1385
HpaII CCGG 2 cut(s) 476, 1376
HphI GGTGA 2 cut(s) 287, 777
Hpy166II GTNNAC 3 cut(s) 57, 348, 436
Hpy188I TCNGA 5 cut(s) 138, 148, 594, 1089, 1195
Hpy188III TCNNGA 6 cut(s) 94, 452, 734, 908, 1292, 1304
Hpy8I GTNNAC 3 cut(s) 57, 348, 436
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 3 cut(s) 436, 811, 963
HpyCH4III ACNGT 4 cut(s) 946, 1048, 1153, 1364
HpyCH4IV ACGT 1 cut(s) 1000
HpyCH4V TGCA 5 cut(s) 695, 962, 1007, 1243, 1248
HpyF10VI GCNNNNNNNGC 5 cut(s) 243, 260, 376, 971, 1232
HpyF3I CTNAG 5 cut(s) 137, 423, 540, 660, 1413
HpySE526I ACGT 1 cut(s) 1000
Hsp92I GRCGYC 1 cut(s) 1084
Hsp92II CATG 8 cut(s) 107, 271, 340, 737, 986, 1023, 1040, 1258
HspAI GCGC 1 cut(s) 683
Kzo9I GATC 3 cut(s) 570, 643, 1342
LmnI GCTCC 5 cut(s) 130, 337, 977, 1058, 1321
Lsp1109I GCAGC 7 cut(s) 244, 406, 667, 974, 991, 1219, 1356
LweI GCATC 4 cut(s) 184, 402, 682, 1219
MaeI CTAG 4 cut(s) 243, 630, 792, 1397
MaeII ACGT 1 cut(s) 1000
MaeIII GTNAC 2 cut(s) 899, 1075
MalI GATC 3 cut(s) 572, 645, 1344
MboI GATC 3 cut(s) 570, 643, 1342
MboII GAAGA 7 cut(s) 35, 152, 195, 400, 488, 768, 1352
MflI RGATCY 1 cut(s) 1342
MhlI GDGCHC 5 cut(s) 228, 615, 753, 982, 1063
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 7 cut(s) 64, 501, 514, 574, 580, 717, 834
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 2 cut(s) 108, 604
MmeI TCCRAC 1 cut(s) 976
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
MseI TTAA 4 cut(s) 194, 579, 870, 1011
MslI CAYNNNNRTG 3 cut(s) 410, 690, 1253
Msp20I TGGCCA 1 cut(s) 374
MspA1I CMGCKG 2 cut(s) 257, 845
MspI CCGG 2 cut(s) 476, 1376
MspR9I CCNGG 3 cut(s) 376, 476, 1200
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 2 cut(s) 376, 1200
MwoI GCNNNNNNNGC 5 cut(s) 243, 260, 376, 971, 1232
NciI CCSGG 1 cut(s) 476
NcoI CCATGG 2 cut(s) 103, 1019
NdeII GATC 3 cut(s) 570, 643, 1342
NlaIII CATG 8 cut(s) 107, 271, 340, 737, 986, 1023, 1040, 1258
NlaIV GGNNCC 3 cut(s) 109, 132, 1323
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 899
NsbI TGCGCA 1 cut(s) 684
NspI RCATGY 1 cut(s) 1040
NspV TTCGAA 1 cut(s) 386
PagI TCATGA 1 cut(s) 733
PaqCI CACCTGC 1 cut(s) 259
PceI AGGCCT 1 cut(s) 1171
PciI ACATGT 1 cut(s) 1036
PcsI WCGNNNNNNNCGW 2 cut(s) 392, 1283
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 5 cut(s) 141, 459, 737, 1288, 1385
PflMI CCANNNNNTGG 1 cut(s) 1070
PkrI GCNGC 9 cut(s) 256, 259, 262, 421, 682, 964, 1006, 1234, 1371
PleI GAGTC 2 cut(s) 107, 603
PpsI GAGTC 2 cut(s) 107, 603
PscI ACATGT 1 cut(s) 1036
PshAI GACNNNNGTC 1 cut(s) 431
Psp124BI GAGCTC 2 cut(s) 753, 982
Psp6I CCWGG 2 cut(s) 374, 1198
PspFI CCCAGC 2 cut(s) 608, 841
PspGI CCWGG 2 cut(s) 374, 1198
PspN4I GGNNCC 3 cut(s) 109, 132, 1323
PsrI GAACNNNNNNTAC 1 cut(s) 1386
PstNI CAGNNNCTG 2 cut(s) 1093, 1199
PsuI RGATCY 1 cut(s) 1342
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 1 cut(s) 881
RsaNI GTAC 1 cut(s) 880
RseI CAYNNNNRTG 3 cut(s) 410, 690, 1253
SacI GAGCTC 2 cut(s) 753, 982
SalI GTCGAC 1 cut(s) 55
SaqAI TTAA 4 cut(s) 194, 579, 870, 1011
SatI GCNGC 9 cut(s) 255, 258, 261, 420, 681, 963, 1005, 1233, 1370
Sau3AI GATC 3 cut(s) 570, 643, 1342
ScaI AGTACT 1 cut(s) 881
SchI GAGTC 2 cut(s) 108, 604
ScrFI CCNGG 3 cut(s) 376, 476, 1200
SduI GDGCHC 5 cut(s) 228, 615, 753, 982, 1063
SfaNI GCATC 4 cut(s) 184, 402, 682, 1219
SfcI CTRYAG 2 cut(s) 361, 1047
SfuI TTCGAA 1 cut(s) 386
SmiMI CAYNNNNRTG 3 cut(s) 410, 690, 1253
SmlI CTYRAG 1 cut(s) 913
SmoI CTYRAG 1 cut(s) 913
Sse9I AATT 7 cut(s) 64, 501, 514, 574, 580, 717, 834
SseBI AGGCCT 1 cut(s) 1171
SsiI CCGC 5 cut(s) 173, 255, 261, 507, 863
SspI AATATT 1 cut(s) 470
SspMI CTAG 4 cut(s) 243, 630, 792, 1397
SstI GAGCTC 2 cut(s) 753, 982
StuI AGGCCT 1 cut(s) 1171
StyD4I CCNGG 3 cut(s) 374, 474, 1198
StyI CCWWGG 3 cut(s) 103, 317, 1019
TaaI ACNGT 4 cut(s) 946, 1048, 1153, 1364
TaiI ACGT 1 cut(s) 1003
TaqI TCGA 4 cut(s) 56, 386, 715, 853
TasI AATT 7 cut(s) 64, 501, 514, 574, 580, 717, 834
TatI WGTACW 1 cut(s) 879
TauI GCSGC 2 cut(s) 257, 263
TfiI GAWTC 5 cut(s) 141, 459, 737, 1288, 1385
Tru1I TTAA 4 cut(s) 194, 579, 870, 1011
Tru9I TTAA 4 cut(s) 194, 579, 870, 1011
TscAI CASTG 4 cut(s) 38, 678, 1075, 1342
TseFI GTSAC 1 cut(s) 899
TseI GCWGC 7 cut(s) 257, 419, 680, 962, 1004, 1232, 1369
Tsp45I GTSAC 1 cut(s) 899
TspDTI ATGAA 5 cut(s) 729, 790, 1271, 1287, 1398
TspGWI ACGGA 2 cut(s) 42, 829
TspRI CASTG 4 cut(s) 38, 678, 1075, 1342
Van91I CCANNNNNTGG 1 cut(s) 1070
XceI RCATGY 1 cut(s) 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 1196
XmiI GTMKAC 2 cut(s) 56, 435
XspI CTAG 4 cut(s) 243, 630, 792, 1397
ZrmI AGTACT 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.