pycom07g01360

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005826
Physical Location & Seq
Forward (+)
1027002 .. 1029451
2450 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGGACAACAATAACGATTTCACTTTTTGCCAGGTTGGCCCACCAGGGATTCAGGATGATCATGAGACAAATAAGCTCACTTCAGATATAGAAAGTATTACCATAAATGATGGATTGTCAAATGGCACTAGTAGTAATGAGAATAGAGGTGTTCTGTGGCAAGGTGGTTTACCAGGAGGAGCCACTTCCAAAAAACAGGAAACAGTTGGTTCTTCGTCTGTCAGCGTCATTGATGCATCATCCATGAAAAAGAAATCTGAAGTTCCAAAGCAACTAAAATCAGGTGATGCTGGAAACTCAGTCAAGCCTGCAACTCGTGCTAAAGTACCTTTTGAGAAGGGATATAGCCAAATGGATTGGCTCAAACTTACTCGAACACATCCTGACCTTGCAGGCTTAAAGGGACAGTCAAATAAGAGGCTTATTTCACTGAATGAAGTTAAACAGCACCAAAAAGGAGATTCAATGTGGACCGTTCTAAAAGGCCGCGTGTACAATTTGTCTCCATACATAAGATTTCACCCGGGAGGTGTTGATATTCTAAAGAAGGCTGTGGGAAAAGACTGCACATCGTTATTCAATAAATACCATGCTTGGGTGAATGCTGAATTTTTACTCGAGAAATGTCTCGTTGGTACTTTTTGA

Protein Analysis

215

Amino Acids

23.42

Weight (kDa)

9.15

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 144 - 213 1.5e-22 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 489
AciI CCGC 1 cut(s) 487
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 2 cut(s) 66, 279
AfaI GTAC 3 cut(s) 327, 494, 637
AfiI CCNNNNNNNGG 1 cut(s) 595
AgsI TTSAA 2 cut(s) 465, 580
AhlI ACTAGT 1 cut(s) 128
AjnI CCWGG 3 cut(s) 30, 43, 172
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 3 cut(s) 59, 507, 632
Ama87I CYCGRG 2 cut(s) 523, 617
AoxI GGCC 2 cut(s) 37, 484
ApoI RAATTY 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 38, 471
AsuC2I CCSGG 2 cut(s) 524, 525
AsuHPI GGTGA 3 cut(s) 296, 512, 610
AvaI CYCGRG 2 cut(s) 523, 617
AvaII GGWCC 1 cut(s) 471
BauI CACGAG 1 cut(s) 315
BccI CCATC 1 cut(s) 104
BciT130I CCWGG 3 cut(s) 32, 45, 174
BclI TGATCA 1 cut(s) 58
BcnI CCSGG 2 cut(s) 524, 525
BcoDI GTCTC 3 cut(s) 59, 507, 632
BcuI ACTAGT 1 cut(s) 128
BfaI CTAG 1 cut(s) 129
BglI GCCNNNNNGGC 1 cut(s) 36
BisI GCNGC 1 cut(s) 487
BlsI GCNGC 1 cut(s) 488
Bme1390I CCNGG 5 cut(s) 32, 45, 174, 524, 525
Bme18I GGWCC 1 cut(s) 471
BmeT110I CYCGRG 2 cut(s) 523, 617
BmgT120I GGNCC 2 cut(s) 38, 471
BmiI GGNNCC 1 cut(s) 181
BmrFI CCNGG 5 cut(s) 32, 45, 174, 524, 525
BmsI GCATC 3 cut(s) 223, 245, 277
BpuMI CCSGG 2 cut(s) 524, 525
BsaJI CCNNGG 2 cut(s) 44, 523
Bsc4I CCNNNNNNNGG 1 cut(s) 595
BseBI CCWGG 3 cut(s) 32, 45, 174
BseDI CCNNGG 2 cut(s) 44, 523
BseGI GGATG 3 cut(s) 61, 239, 379
BseLI CCNNNNNNNGG 1 cut(s) 595
BseMII CTCAG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 2 cut(s) 39, 486
BsiHKCI CYCGRG 2 cut(s) 523, 617
BsiSI CCGG 1 cut(s) 524
BslFI GGGAC 1 cut(s) 417
BslI CCNNNNNNNGG 1 cut(s) 595
BsmAI GTCTC 3 cut(s) 59, 507, 632
BsmFI GGGAC 1 cut(s) 417
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 2 cut(s) 39, 486
BsoBI CYCGRG 2 cut(s) 523, 617
Bsp1407I TGTACA 1 cut(s) 492
Bsp143I GATC 1 cut(s) 58
BspACI CCGC 1 cut(s) 487
BspANI GGCC 2 cut(s) 39, 486
BspCNI CTCAG 1 cut(s) 311
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 181
BsrGI TGTACA 1 cut(s) 492
BssECI CCNNGG 2 cut(s) 44, 523
BssMI GATC 1 cut(s) 58
BssSI CACGAG 1 cut(s) 315
Bst2BI CACGAG 1 cut(s) 315
Bst2UI CCWGG 3 cut(s) 32, 45, 174
Bst4CI ACNGT 3 cut(s) 205, 408, 475
BstAPI GCANNNNNTGC 1 cut(s) 317
BstAUI TGTACA 1 cut(s) 492
BstC8I GCNNGC 2 cut(s) 309, 394
BstDEI CTNAG 1 cut(s) 298
BstF5I GGATG 3 cut(s) 61, 239, 379
BstFNI CGCG 1 cut(s) 489
BstKTI GATC 1 cut(s) 61
BstMAI GTCTC 3 cut(s) 59, 507, 632
BstMBI GATC 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 36, 317
BstNI CCWGG 3 cut(s) 32, 45, 174
BstSCI CCNGG 5 cut(s) 30, 43, 172, 522, 523
BstUI CGCG 1 cut(s) 489
BsuRI GGCC 2 cut(s) 39, 486
BtsCI GGATG 3 cut(s) 61, 239, 379
BtsIMutI CAGTG 1 cut(s) 428
Cac8I GCNNGC 2 cut(s) 309, 394
CciI TCATGA 1 cut(s) 61
Cfr13I GGNCC 2 cut(s) 38, 471
Cfr9I CCCGGG 1 cut(s) 523
CseI GACGC 1 cut(s) 214
Csp6I GTAC 3 cut(s) 326, 493, 636
CviAII CATG 3 cut(s) 62, 244, 590
CviQI GTAC 3 cut(s) 326, 493, 636
DdeI CTNAG 1 cut(s) 298
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 471
Eco57I CTGAAG 2 cut(s) 66, 279
Eco88I CYCGRG 2 cut(s) 523, 617
EcoRII CCWGG 3 cut(s) 30, 43, 172
EcoT22I ATGCAT 1 cut(s) 238
FaeI CATG 3 cut(s) 65, 247, 593
FaiI YATR 8 cut(s) 63, 89, 104, 245, 345, 508, 512, 591
FaqI GGGAC 1 cut(s) 417
FatI CATG 3 cut(s) 61, 243, 589
FbaI TGATCA 1 cut(s) 58
Fnu4HI GCNGC 1 cut(s) 487
FokI GGATG 3 cut(s) 68, 226, 366
Fsp4HI GCNGC 1 cut(s) 487
FspBI CTAG 1 cut(s) 129
GluI GCNGC 1 cut(s) 487
HaeIII GGCC 2 cut(s) 39, 486
HapII CCGG 1 cut(s) 524
HgaI GACGC 1 cut(s) 214
Hin1II CATG 3 cut(s) 65, 247, 593
HinfI GANTC 2 cut(s) 49, 461
HpaII CCGG 1 cut(s) 524
HphI GGTGA 3 cut(s) 296, 512, 610
Hpy166II GTNNAC 3 cut(s) 170, 471, 493
Hpy188I TCNGA 2 cut(s) 85, 259
Hpy188III TCNNGA 4 cut(s) 53, 62, 383, 619
Hpy8I GTNNAC 3 cut(s) 170, 471, 493
HpyAV CCTTC 2 cut(s) 331, 541
HpyCH4III ACNGT 3 cut(s) 205, 408, 475
HpyCH4V TGCA 4 cut(s) 236, 311, 392, 567
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 317
HpyF3I CTNAG 1 cut(s) 298
Hsp92II CATG 3 cut(s) 65, 247, 593
Ksp22I TGATCA 1 cut(s) 58
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 1 cut(s) 179
LweI GCATC 3 cut(s) 223, 245, 277
MaeI CTAG 1 cut(s) 129
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 1 cut(s) 204
MluCI AATT 2 cut(s) 496, 608
MnlI CCTC 4 cut(s) 140, 170, 411, 521
Mph1103I ATGCAT 1 cut(s) 238
MseI TTAA 2 cut(s) 398, 441
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 5 cut(s) 32, 45, 174, 524, 525
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 3 cut(s) 32, 45, 174
MvnI CGCG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 36, 317
NciI CCSGG 2 cut(s) 524, 525
NdeII GATC 1 cut(s) 58
NlaIII CATG 3 cut(s) 65, 247, 593
NlaIV GGNNCC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 238
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 1 cut(s) 61
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 2 cut(s) 49, 461
PkrI GCNGC 1 cut(s) 488
Psp6I CCWGG 3 cut(s) 30, 43, 172
PspGI CCWGG 3 cut(s) 30, 43, 172
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 2 cut(s) 38, 471
RsaI GTAC 3 cut(s) 327, 494, 637
RsaNI GTAC 3 cut(s) 326, 493, 636
SaqAI TTAA 2 cut(s) 398, 441
SatI GCNGC 1 cut(s) 487
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 2 cut(s) 38, 471
ScrFI CCNGG 5 cut(s) 32, 45, 174, 524, 525
SetI ASST 8 cut(s) 36, 78, 151, 166, 286, 331, 390, 532
SfaNI GCATC 3 cut(s) 223, 245, 277
Sfr274I CTCGAG 1 cut(s) 617
SinI GGWCC 1 cut(s) 471
SlaI CTCGAG 1 cut(s) 617
SmaI CCCGGG 1 cut(s) 525
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 128
Sse9I AATT 2 cut(s) 496, 608
SsiI CCGC 1 cut(s) 487
SspMI CTAG 1 cut(s) 129
StyD4I CCNGG 5 cut(s) 30, 43, 172, 522, 523
TaaI ACNGT 3 cut(s) 205, 408, 475
TaqI TCGA 2 cut(s) 373, 618
TasI AATT 2 cut(s) 496, 608
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 2 cut(s) 49, 461
Tru1I TTAA 2 cut(s) 398, 441
Tru9I TTAA 2 cut(s) 398, 441
TscAI CASTG 1 cut(s) 435
TspDTI ATGAA 2 cut(s) 260, 450
TspMI CCCGGG 1 cut(s) 523
TspRI CASTG 1 cut(s) 435
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 1 cut(s) 608
XhoI CTCGAG 1 cut(s) 617
XmaI CCCGGG 1 cut(s) 523
XspI CTAG 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.