Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0003004

Physical Location & Seq

Genomic Coordinates Forward (+)

1043517 .. 1049283

Gene Structure 5767 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1383 bp

ATGAAGCAACAACAGCAGTGCGGAAACAGCGCCATCTATGGAAACGTGTTGCATCAGCTTTGTTTTGACTTGTTCATCCCCCGAAAATTTAGAATTGCCCCTAGAGTTTGGCCACCCCTCTCTCTCTGTCATCCGACTCATCCCACTCAGCTTTTGTTTTCGATACTGTCTCTGTCTATATGTAAACAAATTGGTTTCCTCCAACGAATCCCTCTCTCACATACCACATATCTCTCTTCATCCTCCCTCGAAAAGTCTCTCCTTTTTTTTTTCTGCTTCTCTCGGATTCTCCTTTTGATATCTCCGCAACTTTGCTTTTGGGAAAGTCAAGAGGAGGAGATGTCGAAAGCTGGAGCATTGGATCTCGCATCGGGTCTCGGTGGAAAAATCGACAAAACCGATGTTCTCTCCGCTGTTGAAAAGTATGAGAAGTATCATGTCTGTTATGGAGGGGATGAGGAGGCTAGAAAGTCCAACTACACTGACATGGTTAATAAATACTATGATCTGGTTACCAGCTTTTATGAGTATGGCTGGGGAGAGTCTTTCCACTTTGCACCCAGATGGAATGGGGAGTCTCTTCGAGAGAGTATCAAGCGACACGAACACTTCCTTGCTTTACAACTAGGACTCGAACCTGGGCAGAAAGTTTTGGATGTAGGTTGTGGAATTGGTGGACCACTGAGAGAAATATCTCGCTTCAGCTCAACATCAGTTACTGGGTTGAACAACAACGAATATCAGATCACAAGAGGAAAGGAACTAAACCGTATTGCAGGAGTGGACAAGACCTGCAACTTTGTTAAGGCTGACTTCATGAAACTACCATTTCCTGAAAATTCATTTGATGCAGTATATGCAATTGAAGCGACCTGCCATGCACCAGATGCATATGGGTGCTACAAGGAGATTTACAGAGTATTAAAACCCGGTCAATGTTTTGCTGCATATGAGTGGTGCATGACTAATGCATTTGATCCCGATAACCAAGAACATAAAAAAATCAAGGCAGAAATTGAGATTGGTGATGGCCTCCCAGACATCAGATTGACAGGAAAGTGTCTTGAAGCTTTGAAACAAGCAGGTTTTGAGGTCGTATGGGAGAAAGATCTTGCGGCGGAGTCACCTCTTCCGTGGTACTTGCCTTTGGACAAAAGTCGGATTTCACTTAGTAGCTTTCGTCTAACCACTGCTGGGCGTTTTGTTACTAGAAACTTGGTTAAGGTTTTAGAGTATATTGGAGTTGCACCAGCAGGAAGCCAAAGAGTTCAAGATTTTCTGGAGAAAGCAGCCGTAGGGCTAGTCGAAGGCGGAAAAAAAGAGATTTTCACACCGATGTATTTCTTCCTGGCCCAAAAGCCACTCACAGAGGAGAGTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005911 GO:0006629 GO:0006694 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008169 GO:0008202 GO:0008610 GO:0008757 GO:0009058 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0012505 GO:0016125 GO:0016126 GO:0016740 GO:0016741 GO:0022414 GO:0030054 GO:0032259 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0055044 GO:0061458 GO:0071704 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

CMAS Methyltransf_11 Sterol_MT_C

Protein Analysis

461

Amino Acids

52.01

Weight (kDa)

6.4

Isoelectric Point (pI)

38.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CMAS	PF02353	158 - 369	3.3e-09	Mycolic acid cyclopropane synthetase
Methyltransf_23	PF13489	205 - 311	1.8e-07	Methyltransferase domain
Ubie_methyltran	PF01209	208 - 319	8.2e-10	ubiE/COQ5 methyltransferase family
Methyltransf_31	PF13847	212 - 327	7.2e-15	Methyltransferase domain
Methyltransf_25	PF13649	217 - 311	2.7e-18	Methyltransferase domain
Methyltransf_11	PF08241	218 - 313	2.4e-21	Methyltransferase domain
Methyltransf_12	PF08242	218 - 311	1.7e-08	Methyltransferase domain
Sterol_MT_C	PF08498	391 - 454	8.9e-28	Sterol methyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003004)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13710 AT5G13710
fragaria_vesca	FvH4_7g01390 FvH4_7g01390 FvH4_7g01390
malus_domestica	MD02G1307400.v1.1 MD07G1018200.v1.1
prunus_persica	Prupe.2G019200_v2.0.a1 Prupe.2G019200_v2.0.a1 Prupe.2G019200_v2.0.a1 Prupe.2G019200_v2.0.a1
pyrus_communis	pycom02g25870 pycom07g01400
rosa_chinensis	RchiOBHm_Chr1g0317111 RchiOBHm_Chr3g0482141
rosa_laevigata	RLG00000023373 RLG00000030626
rosa_multiflora	Rmu_sc0000192.1_g000025 Rmu_sc0003689.1_g000012
rosa_roxburghii	Rroxscaffold_4G00330650 Rroxscaffold_6G00399690 Rroxscaffold_6G00400060
rosa_rugosa	Rorug01G0014800 Rorug01G0014900 Rorug01G0014900 Rorug01G0014900 Rorug01G0014900 Rorug03G0194900 Rorug03G0195000
rosa_samantha	Rh1BG022300 Rh1CG024000 Rh1DG047600 Rh3AG246200 Rh3BG281200 Rh3CG277300 Rh3DG273600
rosa_wichuraiana	Rw1G001790 Rw3G022010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	800, 881, 1073
AciI	CCGC	6 cut(s)	21, 305, 411, 1115, 1118, 1311
AclWI	GGATC	2 cut(s)	369, 971
AcoI	YGGCCR	1 cut(s)	110
AcsI	RAATTY	2 cut(s)	86, 838
AcuI	CTGAAG	1 cut(s)	685
AfaI	GTAC	1 cut(s)	1139
AfiI	CCNNNNNNNGG	1 cut(s)	1194
AflIII	ACRYGT	1 cut(s)	45
AgsI	TTSAA	6 cut(s)	419, 727, 866, 1067, 1075, 1271
AjnI	CCWGG	2 cut(s)	637, 1347
AjuI	GAANNNNNNNTTGG	2 cut(s)	1005, 1037
AluBI	AGCT	7 cut(s)	58, 151, 350, 519, 705, 1070, 1176
AluI	AGCT	7 cut(s)	58, 151, 350, 519, 705, 1070, 1176
Alw26I	GTCTC	4 cut(s)	174, 261, 380, 582
AlwI	GGATC	2 cut(s)	369, 971
AlwNI	CAGNNNCTG	1 cut(s)	719
AoxI	GGCC	3 cut(s)	110, 1030, 1350
ApeKI	GCWGC	2 cut(s)	944, 1289
ApoI	RAATTY	2 cut(s)	86, 838
AspLEI	GCGC	1 cut(s)	32
AspS9I	GGNCC	2 cut(s)	677, 1351
AsuC2I	CCSGG	1 cut(s)	930
AsuHPI	GGTGA	2 cut(s)	1037, 1116
AvaII	GGWCC	1 cut(s)	677
BalI	TGGCCA	1 cut(s)	112
BbvI	GCAGC	2 cut(s)	931, 1301
BccI	CCATC	3 cut(s)	41, 558, 1022
BceAI	ACGGC	1 cut(s)	1277
BciT130I	CCWGG	2 cut(s)	639, 1349
BcnI	CCSGG	1 cut(s)	930
BcoDI	GTCTC	4 cut(s)	174, 261, 380, 582
BfaI	CTAG	5 cut(s)	102, 465, 626, 1209, 1301
BfoI	RGCGCY	1 cut(s)	33
BfuAI	ACCTGC	3 cut(s)	800, 881, 1073
BglII	AGATCT	1 cut(s)	1108
BisI	GCNGC	3 cut(s)	945, 1116, 1290
BlsI	GCNGC	3 cut(s)	946, 1117, 1291
Bme1390I	CCNGG	3 cut(s)	639, 930, 1349
Bme18I	GGWCC	1 cut(s)	677
BmgT120I	GGNCC	2 cut(s)	677, 1351
BmrFI	CCNGG	3 cut(s)	639, 930, 1349
BmrI	ACTGGG	1 cut(s)	729
BmsI	GCATC	4 cut(s)	61, 377, 838, 877
BmuI	ACTGGG	1 cut(s)	729
BoxI	GACNNNNGTC	1 cut(s)	1155
BpmI	CTGGAG	2 cut(s)	372, 1301
BpuMI	CCSGG	1 cut(s)	930
BsaI	GGTCTC	1 cut(s)	380
BsaJI	CCNNGG	2 cut(s)	638, 1133
BsaXI	ACNNNNNCTCC	3 cut(s)	326, 356, 1364
Bsc4I	CCNNNNNNNGG	1 cut(s)	1194
Bse1I	ACTGG	1 cut(s)	724
BseBI	CCWGG	2 cut(s)	639, 1349
BseDI	CCNNGG	2 cut(s)	638, 1133
BseGI	GGATG	6 cut(s)	75, 130, 139, 239, 460, 661
BseLI	CCNNNNNNNGG	1 cut(s)	1194
BseMII	CTCAG	2 cut(s)	161, 674
BseNI	ACTGG	1 cut(s)	724
BseRI	GAGGAG	3 cut(s)	347, 350, 473
BseXI	GCAGC	2 cut(s)	931, 1301
BseYI	CCCAGC	2 cut(s)	534, 1193
BshFI	GGCC	3 cut(s)	112, 1032, 1352
BsiSI	CCGG	1 cut(s)	930
BslI	CCNNNNNNNGG	1 cut(s)	1194
BsmAI	GTCTC	4 cut(s)	174, 261, 380, 582
BsnI	GGCC	3 cut(s)	112, 1032, 1352
Bso31I	GGTCTC	1 cut(s)	380
Bsp143I	GATC	5 cut(s)	361, 505, 744, 976, 1108
BspACI	CCGC	6 cut(s)	21, 305, 411, 1115, 1118, 1311
BspANI	GGCC	3 cut(s)	112, 1032, 1352
BspCNI	CTCAG	2 cut(s)	160, 675
BspHI	TCATGA	1 cut(s)	816
BspMI	ACCTGC	3 cut(s)	800, 881, 1073
BspPI	GGATC	2 cut(s)	369, 971
BspTNI	GGTCTC	1 cut(s)	380
BsrI	ACTGG	1 cut(s)	724
BssECI	CCNNGG	2 cut(s)	638, 1133
BssMI	GATC	5 cut(s)	361, 505, 744, 976, 1108
Bst2UI	CCWGG	2 cut(s)	639, 1349
Bst4CI	ACNGT	2 cut(s)	168, 770
Bst6I	CTCTTC	3 cut(s)	241, 585, 1134
BstAPI	GCANNNNNTGC	2 cut(s)	857, 887
BstDEI	CTNAG	3 cut(s)	147, 683, 1169
BstDSI	CCRYGG	1 cut(s)	1133
BstEII	GGTNACC	1 cut(s)	511
BstF5I	GGATG	6 cut(s)	75, 130, 139, 239, 460, 661
BstH2I	RGCGCY	1 cut(s)	33
BstHHI	GCGC	1 cut(s)	32
BstKTI	GATC	5 cut(s)	364, 508, 747, 979, 1111
BstMAI	GTCTC	4 cut(s)	174, 261, 380, 582
BstMBI	GATC	5 cut(s)	361, 505, 744, 976, 1108
BstMWI	GCNNNNNNNGC	5 cut(s)	13, 27, 857, 866, 887
BstNI	CCWGG	2 cut(s)	639, 1349
BstPAI	GACNNNNGTC	1 cut(s)	1155
BstPI	GGTNACC	1 cut(s)	511
BstSCI	CCNGG	3 cut(s)	637, 928, 1347
BstV1I	GCAGC	2 cut(s)	931, 1301
BstX2I	RGATCY	2 cut(s)	361, 1108
BstYI	RGATCY	2 cut(s)	361, 1108
BsuRI	GGCC	3 cut(s)	112, 1032, 1352
BtgI	CCRYGG	1 cut(s)	1133
BtsCI	GGATG	6 cut(s)	75, 130, 139, 239, 460, 661
BtsI	GCAGTG	2 cut(s)	23, 1188
BtsIMutI	CAGTG	4 cut(s)	23, 480, 680, 1188
BveI	ACCTGC	3 cut(s)	800, 881, 1073
CaiI	CAGNNNCTG	1 cut(s)	719
CciI	TCATGA	1 cut(s)	816
CfoI	GCGC	1 cut(s)	32
Cfr13I	GGNCC	2 cut(s)	677, 1351
Csp6I	GTAC	1 cut(s)	1138
CviAII	CATG	5 cut(s)	437, 487, 817, 878, 961
CviQI	GTAC	1 cut(s)	1138
DdeI	CTNAG	3 cut(s)	147, 683, 1169
DpnI	GATC	5 cut(s)	363, 507, 746, 978, 1110
DpnII	GATC	5 cut(s)	361, 505, 744, 976, 1108
EaeI	YGGCCR	1 cut(s)	110
Eam1104I	CTCTTC	3 cut(s)	241, 585, 1134
EarI	CTCTTC	3 cut(s)	241, 585, 1134
EciI	GGCGGA	2 cut(s)	1133, 1326
Eco31I	GGTCTC	1 cut(s)	380
Eco32I	GATATC	1 cut(s)	300
Eco47I	GGWCC	1 cut(s)	677
Eco57I	CTGAAG	1 cut(s)	685
Eco91I	GGTNACC	1 cut(s)	511
EcoO65I	GGTNACC	1 cut(s)	511
EcoRII	CCWGG	2 cut(s)	637, 1347
EcoRV	GATATC	1 cut(s)	300
EcoT22I	ATGCAT	2 cut(s)	892, 973
FaeI	CATG	5 cut(s)	440, 490, 820, 881, 964
FalI	AAGNNNNNCTT	2 cut(s)	797, 829
FatI	CATG	5 cut(s)	436, 486, 816, 877, 960
FauNDI	CATATG	2 cut(s)	892, 949
Fnu4HI	GCNGC	3 cut(s)	945, 1116, 1290
FokI	GGATG	6 cut(s)	62, 117, 126, 226, 467, 668
Fsp4HI	GCNGC	3 cut(s)	945, 1116, 1290
FspBI	CTAG	5 cut(s)	102, 465, 626, 1209, 1301
GlaI	GCGC	1 cut(s)	31
GluI	GCNGC	3 cut(s)	945, 1116, 1290
GsaI	CCCAGC	2 cut(s)	538, 1197
GsuI	CTGGAG	2 cut(s)	372, 1301
HaeII	RGCGCY	1 cut(s)	33
HaeIII	GGCC	3 cut(s)	112, 1032, 1352
HapII	CCGG	1 cut(s)	930
HhaI	GCGC	1 cut(s)	32
Hin1II	CATG	5 cut(s)	440, 490, 820, 881, 964
Hin6I	GCGC	1 cut(s)	30
HinP1I	GCGC	1 cut(s)	30
HindIII	AAGCTT	1 cut(s)	1068
HinfI	GANTC	8 cut(s)	136, 207, 286, 542, 575, 630, 1121, 1375
HpaII	CCGG	1 cut(s)	930
HphI	GGTGA	2 cut(s)	1037, 1116
Hpy166II	GTNNAC	3 cut(s)	185, 677, 784
Hpy188I	TCNGA	5 cut(s)	135, 285, 744, 1046, 1161
Hpy188III	TCNNGA	8 cut(s)	329, 584, 817, 833, 980, 1064, 1271, 1280
Hpy8I	GTNNAC	3 cut(s)	185, 677, 784
HpyAV	CCTTC	1 cut(s)	1301
HpyCH4III	ACNGT	2 cut(s)	168, 770
HpyCH4IV	ACGT	1 cut(s)	45
HpyF10VI	GCNNNNNNNGC	5 cut(s)	13, 27, 857, 866, 887
HpyF3I	CTNAG	3 cut(s)	147, 683, 1169
HpySE526I	ACGT	1 cut(s)	45
Hsp92II	CATG	5 cut(s)	440, 490, 820, 881, 964
HspAI	GCGC	1 cut(s)	30
Kzo9I	GATC	5 cut(s)	361, 505, 744, 976, 1108
LmnI	GCTCC	1 cut(s)	353
Lsp1109I	GCAGC	2 cut(s)	931, 1301
LweI	GCATC	4 cut(s)	61, 377, 838, 877
MaeI	CTAG	5 cut(s)	102, 465, 626, 1209, 1301
MaeII	ACGT	1 cut(s)	45
MaeIII	GTNAC	4 cut(s)	511, 715, 1122, 1204
MalI	GATC	5 cut(s)	363, 507, 746, 978, 1110
MboI	GATC	5 cut(s)	361, 505, 744, 976, 1108
MboII	GAAGA	4 cut(s)	228, 572, 1121, 1336
MfeI	CAATTG	1 cut(s)	861
MflI	RGATCY	2 cut(s)	361, 1108
MlsI	TGGCCA	1 cut(s)	112
MluCI	AATT	7 cut(s)	86, 93, 189, 669, 838, 861, 1014
MluNI	TGGCCA	1 cut(s)	112
MlyI	GAGTC	5 cut(s)	130, 551, 584, 624, 1130
MmeI	TCCRAC	4 cut(s)	158, 226, 498, 1139
Mox20I	TGGCCA	1 cut(s)	112
Mph1103I	ATGCAT	2 cut(s)	892, 973
MscI	TGGCCA	1 cut(s)	112
MseI	TTAA	4 cut(s)	492, 804, 923, 1221
MslI	CAYNNNNRTG	5 cut(s)	485, 562, 895, 952, 1334
Msp20I	TGGCCA	1 cut(s)	112
MspA1I	CMGCKG	1 cut(s)	413
MspI	CCGG	1 cut(s)	930
MspR9I	CCNGG	3 cut(s)	639, 930, 1349
MunI	CAATTG	1 cut(s)	861
MvaI	CCWGG	2 cut(s)	639, 1349
MwoI	GCNNNNNNNGC	5 cut(s)	13, 27, 857, 866, 887
NciI	CCSGG	1 cut(s)	930
NdeI	CATATG	2 cut(s)	892, 949
NdeII	GATC	5 cut(s)	361, 505, 744, 976, 1108
NlaIII	CATG	5 cut(s)	440, 490, 820, 881, 964
NmuCI	GTSAC	1 cut(s)	1122
NsiI	ATGCAT	2 cut(s)	892, 973
PagI	TCATGA	1 cut(s)	816
PcsI	WCGNNNNNNNCGW	1 cut(s)	396
PfeI	GAWTC	2 cut(s)	207, 286
PkrI	GCNGC	3 cut(s)	946, 1117, 1291
PleI	GAGTC	5 cut(s)	130, 550, 583, 624, 1129
PpsI	GAGTC	5 cut(s)	130, 550, 583, 624, 1129
PshAI	GACNNNNGTC	1 cut(s)	1155
Psp6I	CCWGG	2 cut(s)	637, 1347
PspEI	GGTNACC	1 cut(s)	511
PspFI	CCCAGC	2 cut(s)	534, 1193
PspGI	CCWGG	2 cut(s)	637, 1347
PspPI	GGNCC	2 cut(s)	677, 1351
PstNI	CAGNNNCTG	1 cut(s)	719
PsuI	RGATCY	2 cut(s)	361, 1108
RsaI	GTAC	1 cut(s)	1139
RsaNI	GTAC	1 cut(s)	1138
RseI	CAYNNNNRTG	5 cut(s)	485, 562, 895, 952, 1334
SaqAI	TTAA	4 cut(s)	492, 804, 923, 1221
SatI	GCNGC	3 cut(s)	945, 1116, 1290
Sau3AI	GATC	5 cut(s)	361, 505, 744, 976, 1108
Sau96I	GGNCC	2 cut(s)	677, 1351
SchI	GAGTC	5 cut(s)	130, 551, 584, 624, 1130
ScrFI	CCNGG	3 cut(s)	639, 930, 1349
SfaNI	GCATC	4 cut(s)	61, 377, 838, 877
SinI	GGWCC	1 cut(s)	677
SmiMI	CAYNNNNRTG	5 cut(s)	485, 562, 895, 952, 1334
Sse9I	AATT	7 cut(s)	86, 93, 189, 669, 838, 861, 1014
SsiI	CCGC	6 cut(s)	21, 305, 411, 1115, 1118, 1311
SspMI	CTAG	5 cut(s)	102, 465, 626, 1209, 1301
StyD4I	CCNGG	3 cut(s)	637, 928, 1347
TaaI	ACNGT	2 cut(s)	168, 770
TaiI	ACGT	1 cut(s)	48
TaqI	TCGA	7 cut(s)	161, 249, 344, 390, 583, 633, 1305
TasI	AATT	7 cut(s)	86, 93, 189, 669, 838, 861, 1014
TauI	GCSGC	1 cut(s)	1118
TfiI	GAWTC	2 cut(s)	207, 286
Tru1I	TTAA	4 cut(s)	492, 804, 923, 1221
Tru9I	TTAA	4 cut(s)	492, 804, 923, 1221
TscAI	CASTG	4 cut(s)	23, 487, 687, 1195
TseFI	GTSAC	1 cut(s)	1122
TseI	GCWGC	2 cut(s)	944, 1289
Tsp45I	GTSAC	1 cut(s)	1122
TspDTI	ATGAA	6 cut(s)	17, 64, 228, 805, 831, 833
TspGWI	ACGGA	1 cut(s)	1122
TspRI	CASTG	4 cut(s)	23, 487, 687, 1195
VpaK11BI	GGWCC	1 cut(s)	677
XapI	RAATTY	2 cut(s)	86, 838
XspI	CTAG	5 cut(s)	102, 465, 626, 1209, 1301
Zsp2I	ATGCAT	2 cut(s)	892, 973

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.