pycom07g02830

Belongs to the EF-1-beta EF-1-delta family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0011718
Physical Location & Seq
Forward (+)
2373887 .. 2374661
775 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 672 bp
ATGGCCATCACCTTCTCAGATCTCTACACCGATGCCGGTCTCAAAGCCCTCGATGAGTTCCTCGCCGGCAAATCTTACATCTCCGGAGACAAGCTGACTTTGGATGACATCAAGGTCTACGCCGCCGTGTTGGAGAAGCCCGCCGGTTCTTTTGCCAATGTCAGCAAGTGGTACAACGCCGTTTCGTCCCAACTCGCTTCCACCTTCCCCGGCAAGGCTGCTGGAGTGAGAGTCAGCAGCGGCAAGGCTGAGGCTCTTGCACCCGCTCCCGCTGCCGCCGCTGGAGGCGATGACGATGACGATTTGGACCTCTTTGGCGACGAGACTGAGGAGGACAAGAAGGCCGAGGCGGAGTTGGCGGCAGCCAAAAAGGCGTCTACCAAGAAGAAGGAGAGTGGAAAATCCTCTGTTCTTTTGGATGTCAAGCCTTGGGACGATGAGACTGACATGAAGAAGCTCGAGGAGGCTGTTCGGAGTGTCAAGATGGAGGGTCTCTTCTGGGGCGCATCGAAATTGGTTTCTGTTGGTTACGGGATCAAGAAGTTGCAGATCATGCTCACCATTGTCGATGACCTTGTTTCCGTCGATGACCTCATCGAAGAGCAGCTTACTGTTGAGCCCCGCAATGAGTACATCCAGAGCTGCGACATTGTTGCCTTCAACAAAATTTAA

Protein Analysis

224

Amino Acids

23.94

Weight (kDa)

4.56

Isoelectric Point (pI)

31.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF1_GNE PF00736 136 - 223 1.5e-31 EF-1 guanine nucleotide exchange domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011718)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12110 AT5G19510
fragaria_vesca FvH4_4g13950
malus_domestica MD02G1099500.v1.1 MD02G1271700.v1.1 MD07G1040800.v1.1
prunus_persica Prupe.5G005100_v2.0.a1
pyrus_communis pycom02g23230 pycom07g02830
rosa_chinensis RchiOBHm_Chr4g0419721
rosa_laevigata RLG00000007801
rosa_multiflora Rmu_sc0016868.1_g000008
rosa_roxburghii Rroxscaffold_5G00362560
rosa_rugosa Rorug04G0160000
rosa_samantha Rh4AG221200 Rh4BG223200 Rh4CG234500 Rh4DG220200
rosa_wichuraiana Rw4G019040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 266
AccI GTMKAC 2 cut(s) 117, 377
AccIII TCCGGA 1 cut(s) 83
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 666
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 2 cut(s) 173, 632
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 1 cut(s) 661
AluBI AGCT 4 cut(s) 94, 457, 607, 642
AluI AGCT 4 cut(s) 94, 457, 607, 642
Alw26I GTCTC 5 cut(s) 44, 81, 317, 434, 497
AlwI GGATC 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 458
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 2 cut(s) 3, 342
ApeKI GCWGC 6 cut(s) 218, 237, 272, 362, 604, 642
ApoI RAATTY 1 cut(s) 666
AspLEI GCGC 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 550
AvaI CYCGRG 1 cut(s) 458
AvaII GGWCC 1 cut(s) 307
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 621
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 6 cut(s) 205, 249, 259, 374, 616, 629
BccI CCATC 2 cut(s) 14, 478
BceAI ACGGC 2 cut(s) 110, 164
BcgI CGANNNNNNTGC 2 cut(s) 635, 669
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 5 cut(s) 44, 81, 317, 434, 497
BglI GCCNNNNNGGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 210
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 458
BmgT120I GGNCC 1 cut(s) 307
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 2 cut(s) 22, 515
BpmI CTGGAG 2 cut(s) 243, 303
Bpu10I CCTNAGC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 210
BsaHI GRCGYC 1 cut(s) 374
BsaI GGTCTC 2 cut(s) 44, 497
BsaJI CCNNGG 3 cut(s) 208, 345, 428
BsaWI WCCGGW 1 cut(s) 83
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse118I RCCGGY 3 cut(s) 35, 65, 143
Bse3DI GCAATG 1 cut(s) 631
BseAI TCCGGA 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 208, 345, 428
BseGI GGATG 3 cut(s) 109, 424, 633
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 3 cut(s) 30, 240, 318
BseRI GAGGAG 2 cut(s) 344, 476
BseXI GCAGC 6 cut(s) 205, 249, 259, 374, 616, 629
BshFI GGCC 2 cut(s) 5, 344
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 5 cut(s) 36, 66, 84, 144, 210
BslFI GGGAC 2 cut(s) 172, 446
BslI CCNNNNNNNGG 1 cut(s) 214
BsmAI GTCTC 5 cut(s) 44, 81, 317, 434, 497
BsmFI GGGAC 2 cut(s) 172, 446
BsnI GGCC 2 cut(s) 5, 344
Bso31I GGTCTC 2 cut(s) 44, 497
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 1 cut(s) 621
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 3 cut(s) 19, 534, 549
BspANI GGCC 2 cut(s) 5, 344
BspCNI CTCAG 3 cut(s) 29, 241, 319
BspEI TCCGGA 1 cut(s) 83
BspPI GGATC 1 cut(s) 542
BspQI GCTCTTC 1 cut(s) 594
BspTNI GGTCTC 2 cut(s) 44, 497
BsrBI CCGCTC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 631
BsrFI RCCGGY 3 cut(s) 35, 65, 143
BssAI RCCGGY 3 cut(s) 35, 65, 143
BssECI CCNNGG 3 cut(s) 208, 345, 428
BssMI GATC 3 cut(s) 19, 534, 549
BssNI GRCGYC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 428
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 500, 594
BstACI GRCGYC 1 cut(s) 374
BstAPI GCANNNNNTGC 1 cut(s) 553
BstC8I GCNNGC 2 cut(s) 67, 141
BstDEI CTNAG 3 cut(s) 16, 249, 327
BstF5I GGATG 3 cut(s) 109, 424, 633
BstHHI GCGC 1 cut(s) 506
BstKTI GATC 3 cut(s) 22, 537, 552
BstMAI GTCTC 5 cut(s) 44, 81, 317, 434, 497
BstMBI GATC 3 cut(s) 19, 534, 549
BstMWI GCNNNNNNNGC 5 cut(s) 272, 278, 356, 371, 553
BstSCI CCNGG 1 cut(s) 208
BstV1I GCAGC 6 cut(s) 205, 249, 259, 374, 616, 629
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuRI GGCC 2 cut(s) 5, 344
BtgZI GCGATG 1 cut(s) 303
BtsCI GGATG 3 cut(s) 109, 424, 633
Cac8I GCNNGC 2 cut(s) 67, 141
CfoI GCGC 1 cut(s) 506
Cfr10I RCCGGY 3 cut(s) 35, 65, 143
Cfr13I GGNCC 1 cut(s) 307
CseI GACGC 1 cut(s) 363
Csp6I GTAC 2 cut(s) 172, 631
CviAII CATG 2 cut(s) 448, 553
CviQI GTAC 2 cut(s) 172, 631
DdeI CTNAG 3 cut(s) 16, 249, 327
DpnI GATC 3 cut(s) 21, 536, 551
DpnII GATC 3 cut(s) 19, 534, 549
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 500, 594
EarI CTCTTC 2 cut(s) 500, 594
EciI GGCGGA 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 621
Eco31I GGTCTC 2 cut(s) 44, 497
Eco47I GGWCC 1 cut(s) 307
Eco88I CYCGRG 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 621
ErhI CCWWGG 1 cut(s) 428
FaeI CATG 2 cut(s) 451, 556
FaiI YATR 2 cut(s) 449, 554
FalI AAGNNNNNCTT 2 cut(s) 591, 623
FaqI GGGAC 2 cut(s) 172, 446
FatI CATG 2 cut(s) 447, 552
FauI CCCGC 4 cut(s) 148, 271, 277, 629
FblI GTMKAC 2 cut(s) 117, 377
FokI GGATG 3 cut(s) 116, 431, 620
FriOI GRGCYC 1 cut(s) 621
GlaI GCGC 1 cut(s) 505
GsuI CTGGAG 2 cut(s) 243, 303
HaeIII GGCC 2 cut(s) 5, 344
HapII CCGG 5 cut(s) 36, 66, 84, 144, 210
HgaI GACGC 1 cut(s) 363
HhaI GCGC 1 cut(s) 506
Hin1I GRCGYC 1 cut(s) 374
Hin1II CATG 2 cut(s) 451, 556
Hin6I GCGC 1 cut(s) 504
HinP1I GCGC 1 cut(s) 504
HinfI GANTC 1 cut(s) 231
HpaII CCGG 5 cut(s) 36, 66, 84, 144, 210
HphI GGTGA 1 cut(s) 550
Hpy166II GTNNAC 2 cut(s) 118, 378
Hpy188I TCNGA 2 cut(s) 19, 474
Hpy188III TCNNGA 4 cut(s) 84, 481, 538, 637
Hpy8I GTNNAC 2 cut(s) 118, 378
Hpy99I CGWCG 2 cut(s) 323, 587
HpyAV CCTTC 5 cut(s) 22, 214, 334, 382, 667
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4V TGCA 2 cut(s) 260, 547
HpyF10VI GCNNNNNNNGC 5 cut(s) 272, 278, 356, 371, 553
HpyF3I CTNAG 3 cut(s) 16, 249, 327
Hsp92I GRCGYC 1 cut(s) 374
Hsp92II CATG 2 cut(s) 451, 556
HspAI GCGC 1 cut(s) 504
Kpn2I TCCGGA 1 cut(s) 83
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 19, 534, 549
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 9 cut(s) 49, 79, 97, 157, 207, 223, 267, 484, 650
Lsp1109I GCAGC 6 cut(s) 205, 249, 259, 374, 616, 629
LweI GCATC 2 cut(s) 22, 515
MaeIII GTNAC 1 cut(s) 527
MalI GATC 3 cut(s) 21, 536, 551
MbiI CCGCTC 1 cut(s) 266
MboI GATC 3 cut(s) 19, 534, 549
MboII GAAGA 4 cut(s) 397, 463, 487, 611
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 621
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 512, 666
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 240
MmeI TCCRAC 1 cut(s) 111
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 83
MroNI GCCGGC 1 cut(s) 65
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 670
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 3 cut(s) 240, 272, 281
MspI CCGG 5 cut(s) 36, 66, 84, 144, 210
MspR9I CCNGG 1 cut(s) 210
MwoI GCNNNNNNNGC 5 cut(s) 272, 278, 356, 371, 553
NaeI GCCGGC 1 cut(s) 67
NciI CCSGG 1 cut(s) 210
NdeII GATC 3 cut(s) 19, 534, 549
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 2 cut(s) 451, 556
NmeAIII GCCGAG 1 cut(s) 370
PaeR7I CTCGAG 1 cut(s) 458
PciSI GCTCTTC 1 cut(s) 594
PdiI GCCGGC 1 cut(s) 67
PleI GAGTC 1 cut(s) 239
PpsI GAGTC 1 cut(s) 239
PspPI GGNCC 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 458
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 173, 632
RsaNI GTAC 2 cut(s) 172, 631
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 1 cut(s) 670
Sau3AI GATC 3 cut(s) 19, 534, 549
Sau96I GGNCC 1 cut(s) 307
SchI GAGTC 1 cut(s) 240
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 2 cut(s) 22, 515
Sfr274I CTCGAG 1 cut(s) 458
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 458
SmoI CTYRAG 1 cut(s) 458
Sse9I AATT 2 cut(s) 512, 666
StyD4I CCNGG 1 cut(s) 208
StyI CCWWGG 1 cut(s) 428
TaaI ACNGT 1 cut(s) 613
TaqI TCGA 6 cut(s) 51, 459, 509, 567, 585, 597
TasI AATT 2 cut(s) 512, 666
TatI WGTACW 1 cut(s) 630
TauI GCSGC 5 cut(s) 125, 243, 278, 281, 362
Tru1I TTAA 1 cut(s) 670
Tru9I TTAA 1 cut(s) 670
TseI GCWGC 6 cut(s) 218, 237, 272, 362, 604, 642
TspDTI ATGAA 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 1 cut(s) 666
XhoI CTCGAG 1 cut(s) 458
XmiI GTMKAC 2 cut(s) 117, 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.