pycom07g07510

Belongs to the ALG6 ALG8 glucosyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007330
Physical Location & Seq
Forward (+)
6821893 .. 6823318
1426 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g07510.1

Sequence Viewer

Length: 462 bp
ATGGAAGCTCAAACCGCCCCGAAACTCCCAAACCCTCCGAAACCCCGCAACCTCATCACAGAGCTACGGGGGTTCTTCGACATCACCGCCTGCGTAAAGCTGCTTCTAATCCTAGCCTACAAAAGCACAGACTATGAGGTCCACCGCCACTGGCTAGCGACAACCCACCTCTGCCTCTTTCCCATTGGTACTCCTACGAGACGAGCATTTGGACGGACCCTCGACTGCCCGCCATTCTTCGCCTACATCGAGCTCTTCCTTTCCGTTTTCGCCAATCTCATCGACCGACAAATCGGTCACCTCCAAAAGGGCTTGAACTCCAGCGCAAACACAGTTCTTTACTTCCAAAGAATCTTGGGTGGACATGGTAATAATCCCAGCAAGCACTGGGCATATGGGTGTTCTTCTTGGACATGTAGCAACAATTGCCGAGTTGAAACCCGGGGTCATGTCGGTACATGA

Protein Analysis

154

Amino Acids

17.26

Weight (kDa)

9.32

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alg6_Alg8 PF03155 30 - 118 1.1e-19 ALG6, ALG8 glycosyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 137, 294
AciI CCGC 5 cut(s) 15, 46, 87, 145, 230
AfaI GTAC 2 cut(s) 190, 457
AfiI CCNNNNNNNGG 1 cut(s) 307
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 2 cut(s) 316, 437
AluBI AGCT 4 cut(s) 8, 64, 100, 253
AluI AGCT 4 cut(s) 8, 64, 100, 253
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 193
Ama87I CYCGRG 1 cut(s) 441
ApeKI GCWGC 1 cut(s) 100
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 2 cut(s) 139, 216
AsuC2I CCSGG 2 cut(s) 442, 443
AsuHPI GGTGA 2 cut(s) 76, 290
AsuNHI GCTAGC 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 441
AvaII GGWCC 2 cut(s) 139, 216
BanII GRGCYC 1 cut(s) 255
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 87
BcnI CCSGG 2 cut(s) 442, 443
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 113, 155
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme1390I CCNGG 2 cut(s) 442, 443
Bme18I GGWCC 2 cut(s) 139, 216
BmeT110I CYCGRG 1 cut(s) 441
BmgT120I GGNCC 2 cut(s) 139, 216
BmiI GGNNCC 1 cut(s) 218
BmrFI CCNGG 2 cut(s) 442, 443
BmrI ACTGGG 1 cut(s) 397
BmtI GCTAGC 1 cut(s) 158
BmuI ACTGGG 1 cut(s) 397
BpmI CTGGAG 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 442, 443
BsaJI CCNNGG 2 cut(s) 441, 442
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 2 cut(s) 155, 392
BseDI CCNNGG 2 cut(s) 441, 442
BseLI CCNNNNNNNGG 1 cut(s) 307
BseNI ACTGG 2 cut(s) 155, 392
BseXI GCAGC 1 cut(s) 87
BseYI CCCAGC 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 286
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 1 cut(s) 441
BsiSI CCGG 1 cut(s) 442
BslI CCNNNNNNNGG 1 cut(s) 307
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsoBI CYCGRG 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 255
BspACI CCGC 5 cut(s) 15, 46, 87, 145, 230
BspLI GGNNCC 1 cut(s) 218
BspOI GCTAGC 1 cut(s) 158
BspQI GCTCTTC 1 cut(s) 260
BsrI ACTGG 2 cut(s) 155, 392
BssECI CCNNGG 2 cut(s) 441, 442
Bst4CI ACNGT 1 cut(s) 334
Bst6I CTCTTC 1 cut(s) 260
BstAPI GCANNNNNTGC 1 cut(s) 426
BstC8I GCNNGC 4 cut(s) 91, 156, 230, 383
BstEII GGTNACC 1 cut(s) 296
BstENI CCTNNNNNAGG 1 cut(s) 305
BstHHI GCGC 1 cut(s) 326
BstMAI GTCTC 1 cut(s) 193
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 2 cut(s) 14, 426
BstNSI RCATGY 1 cut(s) 417
BstPI GGTNACC 1 cut(s) 296
BstSCI CCNGG 2 cut(s) 440, 441
BstV1I GCAGC 1 cut(s) 87
BtsIMutI CAGTG 2 cut(s) 148, 385
Cac8I GCNNGC 4 cut(s) 91, 156, 230, 383
CfoI GCGC 1 cut(s) 326
Cfr13I GGNCC 2 cut(s) 139, 216
Cfr9I CCCGGG 1 cut(s) 441
Csp6I GTAC 2 cut(s) 189, 456
CviAII CATG 4 cut(s) 365, 414, 449, 459
CviJI RGCY 7 cut(s) 8, 64, 100, 116, 154, 253, 312
CviKI_1 RGCY 7 cut(s) 8, 64, 100, 116, 154, 253, 312
CviQI GTAC 2 cut(s) 189, 456
DrdI GACNNNNNNGTC 2 cut(s) 137, 294
DseDI GACNNNNNNGTC 2 cut(s) 137, 294
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 139, 216
Eco53kI GAGCTC 1 cut(s) 253
Eco88I CYCGRG 1 cut(s) 441
Eco91I GGTNACC 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 296
EcoT38I GRGCYC 1 cut(s) 255
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 4 cut(s) 368, 417, 452, 462
FaiI YATR 7 cut(s) 135, 366, 394, 396, 415, 450, 460
FatI CATG 4 cut(s) 364, 413, 448, 458
FauI CCCGC 2 cut(s) 53, 237
FauNDI CATATG 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 101
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 101
FspBI CTAG 2 cut(s) 113, 155
GlaI GCGC 1 cut(s) 325
GluI GCNGC 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 381
GsuI CTGGAG 1 cut(s) 304
HapII CCGG 1 cut(s) 442
HhaI GCGC 1 cut(s) 326
Hin1II CATG 4 cut(s) 368, 417, 452, 462
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 1 cut(s) 351
HpaII CCGG 1 cut(s) 442
HphI GGTGA 2 cut(s) 76, 290
Hpy166II GTNNAC 2 cut(s) 142, 362
Hpy188I TCNGA 1 cut(s) 39
Hpy8I GTNNAC 2 cut(s) 142, 362
HpyCH4III ACNGT 1 cut(s) 334
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 426
Hsp92II CATG 4 cut(s) 368, 417, 452, 462
HspAI GCGC 1 cut(s) 324
LguI GCTCTTC 1 cut(s) 260
LpnPI CCDG 6 cut(s) 103, 136, 334, 373, 391, 455
Lsp1109I GCAGC 1 cut(s) 87
MaeI CTAG 2 cut(s) 113, 155
MaeIII GTNAC 1 cut(s) 296
MboII GAAGA 4 cut(s) 67, 229, 247, 396
MfeI CAATTG 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 1 cut(s) 424
MnlI CCTC 7 cut(s) 45, 62, 130, 179, 185, 230, 311
MslI CAYNNNNRTG 1 cut(s) 397
MspI CCGG 1 cut(s) 442
MspR9I CCNGG 2 cut(s) 442, 443
MunI CAATTG 1 cut(s) 424
MwoI GCNNNNNNNGC 2 cut(s) 14, 426
NciI CCSGG 2 cut(s) 442, 443
NdeI CATATG 1 cut(s) 394
NheI GCTAGC 1 cut(s) 154
NlaIII CATG 4 cut(s) 368, 417, 452, 462
NlaIV GGNNCC 1 cut(s) 218
NmeAIII GCCGAG 1 cut(s) 455
NmuCI GTSAC 1 cut(s) 296
NspI RCATGY 1 cut(s) 417
PciI ACATGT 1 cut(s) 413
PciSI GCTCTTC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PfeI GAWTC 1 cut(s) 351
PkrI GCNGC 1 cut(s) 102
PscI ACATGT 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 296
PspFI CCCAGC 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 218
PspPI GGNCC 2 cut(s) 139, 216
RsaI GTAC 2 cut(s) 190, 457
RsaNI GTAC 2 cut(s) 189, 456
RseI CAYNNNNRTG 1 cut(s) 397
SacI GAGCTC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 260
SatI GCNGC 1 cut(s) 101
Sau96I GGNCC 2 cut(s) 139, 216
ScrFI CCNGG 2 cut(s) 442, 443
SduI GDGCHC 1 cut(s) 255
SetI ASST 8 cut(s) 10, 54, 66, 102, 141, 171, 255, 303
SinI GGWCC 2 cut(s) 139, 216
SmaI CCCGGG 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 397
Sse9I AATT 1 cut(s) 424
SsiI CCGC 5 cut(s) 15, 46, 87, 145, 230
SspMI CTAG 2 cut(s) 113, 155
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 440, 441
TaaI ACNGT 1 cut(s) 334
TaqI TCGA 4 cut(s) 78, 222, 249, 282
TaqII GACCGA 2 cut(s) 284, 300
TasI AATT 1 cut(s) 424
TfiI GAWTC 1 cut(s) 351
TscAI CASTG 2 cut(s) 155, 392
TseFI GTSAC 1 cut(s) 296
TseI GCWGC 1 cut(s) 100
Tsp45I GTSAC 1 cut(s) 296
TspGWI ACGGA 2 cut(s) 229, 253
TspMI CCCGGG 1 cut(s) 441
TspRI CASTG 2 cut(s) 155, 392
VpaK11BI GGWCC 2 cut(s) 139, 216
XagI CCTNNNNNAGG 1 cut(s) 305
XceI RCATGY 1 cut(s) 417
XmaI CCCGGG 1 cut(s) 441
XspI CTAG 2 cut(s) 113, 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.