pycom07g12430

Protein-ribulosamine 3-kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005372
Physical Location & Seq
Forward (+)
13766285 .. 13768229
1945 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 690 bp
ATGTATGAAACTGGGTCAATTCGTGTCCCTAAGCCATTCAAGTTCGGGCCACTACCTACTGGTGGGTCTTACATCATTATGGAATTTATCGAATTTGGTTCATCTAGAGGTAACCAGTCTATTTTAGGCAGGAAGCTTGCTGAAATGCATAAAGCTGGAAAATCTGAAAAAGGTTTTGGTTTTGAAGTTGACAACACTGTAGGCAGCACTCCACAGATAAACACTTGGTCATCAGATTGGATTCAGTTCTATGGGGAGCATAGACTGGGTTATCAGTTGCAGCTGGCATTAGACCAGTACGGTGATAGTACTATATATGAAAAAGGACAAAGATTGATTAAGAGTATGGGATCGCTCTTTGATAACATAGCTATAGAACCATGTTTGTTACATGGGGACTTATGGAGCGGAAATATCAGCGCTGATAAGAATGGCGAGCCTGTTATACTTGATCCAGCATGTTACTATGGACATAACGAGGCAGAATTTGGAATGTCGTGGTGTGCTGGCTTTGGAGGATCTTTCTACGGTTCCTATTTCGAGGTGATGCCAAAACAACCAGGATTTGAAAAGAGGAAGGAGCTATATCTGTTGTATCATTACTTGAATCATTATAACCTCTTTGGGTCGGGTTATCGTTCGTCTTCAATGTCTATAATAGACGATTATCTTCGGATGTTGAAAGCTTAG

Protein Analysis

230

Amino Acids

25.86

Weight (kDa)

5.87

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fructosamin_kin PF03881 1 - 225 1.2e-81 Fructosamine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AccBSI CCGCTC 1 cut(s) 408
AciI CCGC 1 cut(s) 408
AclWI GGATC 3 cut(s) 358, 446, 526
AcsI RAATTY 3 cut(s) 83, 92, 485
AfaI GTAC 2 cut(s) 299, 310
AfeI AGCGCT 1 cut(s) 421
AfiI CCNNNNNNNGG 1 cut(s) 62
AgsI TTSAA 6 cut(s) 40, 185, 569, 607, 648, 682
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 6 cut(s) 136, 155, 283, 371, 583, 686
AluI AGCT 6 cut(s) 136, 155, 283, 371, 583, 686
AlwI GGATC 3 cut(s) 358, 446, 526
Aor51HI AGCGCT 1 cut(s) 421
AoxI GGCC 1 cut(s) 47
ApeKI GCWGC 2 cut(s) 204, 280
ApoI RAATTY 3 cut(s) 83, 92, 485
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 314, 556
BbsI GAAGAC 1 cut(s) 636
BbvI GCAGC 2 cut(s) 216, 292
BciT130I CCWGG 1 cut(s) 561
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 2 cut(s) 198, 372
BfoI RGCGCY 1 cut(s) 423
BisI GCNGC 2 cut(s) 205, 281
BlsI GCNGC 2 cut(s) 206, 282
BmcAI AGTACT 1 cut(s) 310
Bme1390I CCNGG 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 1 cut(s) 561
BmrI ACTGGG 2 cut(s) 21, 275
BmsI GCATC 1 cut(s) 537
BmuI ACTGGG 2 cut(s) 21, 275
BpiI GAAGAC 1 cut(s) 636
Bpu10I CCTNAGC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 1 cut(s) 62
Bse1I ACTGG 5 cut(s) 16, 64, 115, 270, 295
BseBI CCWGG 1 cut(s) 561
BseGI GGATG 1 cut(s) 681
BseLI CCNNNNNNNGG 1 cut(s) 62
BseNI ACTGG 5 cut(s) 16, 64, 115, 270, 295
BseXI GCAGC 2 cut(s) 216, 292
BshFI GGCC 1 cut(s) 49
BslFI GGGAC 2 cut(s) 11, 410
BslI CCNNNNNNNGG 1 cut(s) 62
BsmFI GGGAC 2 cut(s) 11, 410
BsnI GGCC 1 cut(s) 49
Bsp143I GATC 3 cut(s) 350, 451, 518
BspACI CCGC 1 cut(s) 408
BspANI GGCC 1 cut(s) 49
BspLI GGNNCC 1 cut(s) 532
BspPI GGATC 3 cut(s) 358, 446, 526
BsrBI CCGCTC 1 cut(s) 408
BsrI ACTGG 5 cut(s) 16, 64, 115, 270, 295
BssMI GATC 3 cut(s) 350, 451, 518
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 3 cut(s) 199, 302, 530
BstC8I GCNNGC 4 cut(s) 138, 285, 437, 508
BstDEI CTNAG 2 cut(s) 30, 687
BstEII GGTNACC 1 cut(s) 110
BstF5I GGATG 1 cut(s) 681
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 3 cut(s) 353, 454, 521
BstMBI GATC 3 cut(s) 350, 451, 518
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 1 cut(s) 462
BstPI GGTNACC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 559
BstSFI CTRYAG 2 cut(s) 198, 372
BstV1I GCAGC 2 cut(s) 216, 292
BstV2I GAAGAC 1 cut(s) 636
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BsuRI GGCC 1 cut(s) 49
BtsCI GGATG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 4 cut(s) 138, 285, 437, 508
CfoI GCGC 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 298, 309
CviAII CATG 3 cut(s) 381, 392, 459
CviQI GTAC 2 cut(s) 298, 309
DdeI CTNAG 2 cut(s) 30, 687
DpnI GATC 3 cut(s) 352, 453, 520
DpnII GATC 3 cut(s) 350, 451, 518
Eco47III AGCGCT 1 cut(s) 421
Eco91I GGTNACC 1 cut(s) 110
EcoO65I GGTNACC 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 559
EcoT22I ATGCAT 1 cut(s) 150
FaeI CATG 3 cut(s) 384, 395, 462
FaqI GGGAC 2 cut(s) 11, 410
FatI CATG 3 cut(s) 380, 391, 458
Fnu4HI GCNGC 2 cut(s) 205, 281
Fsp4HI GCNGC 2 cut(s) 205, 281
FspBI CTAG 1 cut(s) 105
GlaI GCGC 1 cut(s) 421
GluI GCNGC 2 cut(s) 205, 281
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 1 cut(s) 49
HhaI GCGC 1 cut(s) 422
Hin1II CATG 3 cut(s) 384, 395, 462
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HindIII AAGCTT 2 cut(s) 134, 684
HinfI GANTC 2 cut(s) 241, 607
HphI GGTGA 2 cut(s) 314, 556
Hpy166II GTNNAC 1 cut(s) 190
Hpy188I TCNGA 3 cut(s) 166, 235, 675
Hpy188III TCNNGA 1 cut(s) 105
Hpy8I GTNNAC 1 cut(s) 190
HpyAV CCTTC 1 cut(s) 571
HpyCH4III ACNGT 3 cut(s) 199, 302, 530
HpyCH4V TGCA 2 cut(s) 148, 280
HpyF3I CTNAG 2 cut(s) 30, 687
Hsp92II CATG 3 cut(s) 384, 395, 462
HspAI GCGC 1 cut(s) 420
Kzo9I GATC 3 cut(s) 350, 451, 518
LmnI GCTCC 3 cut(s) 256, 405, 580
Lsp1109I GCAGC 2 cut(s) 216, 292
LweI GCATC 1 cut(s) 537
MaeI CTAG 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 110, 387, 461
MalI GATC 3 cut(s) 352, 453, 520
MbiI CCGCTC 1 cut(s) 408
MboI GATC 3 cut(s) 350, 451, 518
MboII GAAGA 2 cut(s) 636, 662
MflI RGATCY 1 cut(s) 518
MluCI AATT 4 cut(s) 18, 83, 92, 485
MnlI CCTC 6 cut(s) 101, 472, 509, 535, 567, 629
Mph1103I ATGCAT 1 cut(s) 150
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 283
MspR9I CCNGG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 561
NdeII GATC 3 cut(s) 350, 451, 518
NlaIII CATG 3 cut(s) 384, 395, 462
NlaIV GGNNCC 1 cut(s) 532
NsiI ATGCAT 1 cut(s) 150
NspI RCATGY 1 cut(s) 462
PfeI GAWTC 2 cut(s) 241, 607
PkrI GCNGC 2 cut(s) 206, 282
PsiI TTATAA 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 559
PspEI GGTNACC 1 cut(s) 110
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 1 cut(s) 532
PspPI GGNCC 1 cut(s) 47
PsuI RGATCY 1 cut(s) 518
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 2 cut(s) 299, 310
RsaNI GTAC 2 cut(s) 298, 309
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 2 cut(s) 205, 281
Sau3AI GATC 3 cut(s) 350, 451, 518
Sau96I GGNCC 1 cut(s) 47
ScaI AGTACT 1 cut(s) 310
ScrFI CCNGG 1 cut(s) 561
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 2 cut(s) 198, 372
SmiMI CAYNNNNRTG 1 cut(s) 77
Sse9I AATT 4 cut(s) 18, 83, 92, 485
SsiI CCGC 1 cut(s) 408
SspMI CTAG 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 559
TaaI ACNGT 3 cut(s) 199, 302, 530
TaqI TCGA 2 cut(s) 90, 540
TasI AATT 4 cut(s) 18, 83, 92, 485
TatI WGTACW 1 cut(s) 308
TfiI GAWTC 2 cut(s) 241, 607
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 202
TseI GCWGC 2 cut(s) 204, 280
TspDTI ATGAA 3 cut(s) 21, 90, 333
TspRI CASTG 1 cut(s) 202
XapI RAATTY 3 cut(s) 83, 92, 485
XbaI TCTAGA 1 cut(s) 104
XceI RCATGY 1 cut(s) 462
XspI CTAG 1 cut(s) 105
ZrmI AGTACT 1 cut(s) 310
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.