pycom07g14100

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009207
Physical Location & Seq
Forward (+)
15932318 .. 15933932
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1344 bp
ATGGAACATCGATTGACGTGGCTTGTGAACACTTCATCATGCCATCAAACCATCCACTTCATTTTTTACCCTACACGAATATTGCCCCAGTCTAATCAGGACCCGCCATCCCCCAAACTGTCTGTTTTTTTACTGCGCCTCCTCCCGCCCCCTCCTCTCCCTCTCGCCTCTCTCCCTCCACCGGCAAGCCATGCGCCGACGACGTCCCCGTTTTTGAATCTCATCAAGGCCGTCCGTTTTCTCTTCCCCTTCTTTCTCGCTCGTATCTCGAAAGAAAACGGAGACGACGAACGTGTGCACGTCTTGGAGATCAACCTCGTCTCCGCCCAGGGTCTCAAGGCTCCCTCCGTCACCCTCTGCCGCATGCAGACCTACGCTCTCGCCTGGGTCGACTCCGCCCACAAGATCCGCTCTCGGGTCGACAAGGTGGGCGGTGAGAATCCGACATGGAATGATCGGTGCCTCTTCAAGGTCCCGTCCAGATTCCTCTCCAGCGAGACCTCCCAGGTCTCGGTCCAGATCTATGCCGTCGGATGCTTCCGCGATCACCTCGTCGGCACCGTCCGATTTCTGATCAACAACTTTCTCGACGTCTCGTCCACCATTCCATCGTTTACGGCGATCCAAATCCGGCGACCTTCTGGAAGATTCCGAGGCGTGTTGAATGTGGCCGCGTTGGTGATCGACGGCTCGAGTCTGGCTCCTGCAGTGAGCGAGCTGCCTGCGATCGGGTACCGCGACCTGATGGGAATGGGAGAGAGCTTCCGGCGCCGGCGACGCGACAGCATGACAAGCAGATCGAAGAATTACTCCAGCGATTCGAAGGAGAACTCTTGCACTGAATCGACGGAAAACTCGGACTTGAGCGAATCGGCGGCGTCATCACCAGAGACGCCGTTGCCGCCTCTGAAGGAGCTGAACACGATCAGCGAATTGGCGGGAACAAGAAGCCAGGTGTTGAAGGCTCCTCCAACGGACGGCTCGCGTTTCCTGTGCTGCTTTCTGACGCAGAGGAAAATTAAATACAATCCGTCGGATCAGAACGCAGATGGGGCCCACAAACGTAAATTTGTACAAAATATTGTGTGCAAAAAATTATGCAATTGGTCGTGTAATAATAATATGATCAAATTTTCCTCTATATTTGAGGTGTTTCAATGGCAATCCAAGTGGTGGACTGGTGGATTTGCACATTTCTCATTTATTTTTAAGATTTGGTTTTTCATGCATTTTGCATTAATAATGTTCTTTGTATCACATTTACTGTTGCATTTAGAAATTATGGCTGGAGCAATTTTTACCATTGTGACTATGCGAACAAATATAAGTTGGTATTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

448

Amino Acids

50.55

Weight (kDa)

9.55

Isoelectric Point (pI)

51.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 101 - 195 5.5e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 206, 594
Acc65I GGTACC 1 cut(s) 732
AccB1I GGYRCC 4 cut(s) 459, 557, 732, 768
AccB7I CCANNNNNTGG 1 cut(s) 1173
AccBSI CCGCTC 1 cut(s) 411
AccI GTMKAC 2 cut(s) 390, 420
AccII CGCG 5 cut(s) 543, 674, 738, 780, 985
AclWI GGATC 3 cut(s) 400, 616, 1044
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 2 cut(s) 1067, 1130
AcuI CTGAAG 1 cut(s) 929
AcyI GRCGYC 5 cut(s) 203, 591, 769, 878, 893
AfaI GTAC 2 cut(s) 734, 1074
AfiI CCNNNNNNNGG 7 cut(s) 181, 415, 469, 511, 728, 977, 1173
AflIII ACRYGT 1 cut(s) 292
AgsI TTSAA 5 cut(s) 217, 469, 664, 961, 1157
AhdI GACNNNNNGTC 1 cut(s) 595
AjiI CACGTC 2 cut(s) 18, 301
AjnI CCWGG 4 cut(s) 327, 383, 504, 951
AluBI AGCT 3 cut(s) 718, 762, 916
AluI AGCT 3 cut(s) 718, 762, 916
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 7 cut(s) 276, 325, 338, 491, 514, 598, 884
Alw44I GTGCAC 1 cut(s) 296
AlwI GGATC 3 cut(s) 400, 616, 1044
Ama87I CYCGRG 2 cut(s) 414, 691
AoxI GGCC 3 cut(s) 228, 669, 1053
ApaI GGGCCC 1 cut(s) 1057
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 718, 996
ApoI RAATTY 2 cut(s) 1067, 1130
AseI ATTAAT 1 cut(s) 1238
Asp718I GGTACC 1 cut(s) 732
AspLEI GCGC 3 cut(s) 138, 196, 771
AspS9I GGNCC 5 cut(s) 100, 472, 514, 1053, 1054
AsuHPI GGTGA 5 cut(s) 343, 446, 539, 691, 876
AsuII TTCGAA 1 cut(s) 821
AvaI CYCGRG 2 cut(s) 414, 691
AvaII GGWCC 3 cut(s) 100, 472, 514
BaeGI GKGCMC 2 cut(s) 300, 1057
BanI GGYRCC 4 cut(s) 459, 557, 732, 768
BanII GRGCYC 1 cut(s) 1057
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 2 cut(s) 705, 983
BccI CCATC 6 cut(s) 51, 59, 115, 616, 739, 1043
BceAI ACGGC 6 cut(s) 215, 512, 633, 703, 880, 994
BcgI CGANNNNNNTGC 2 cut(s) 704, 738
BciT130I CCWGG 4 cut(s) 329, 385, 506, 953
BclI TGATCA 2 cut(s) 573, 1125
BcoDI GTCTC 7 cut(s) 276, 325, 338, 491, 514, 598, 884
BfmI CTRYAG 1 cut(s) 705
BfoI RGCGCY 1 cut(s) 772
BglII AGATCT 1 cut(s) 519
BisI GCNGC 6 cut(s) 361, 672, 719, 876, 902, 997
BlsI GCNGC 6 cut(s) 362, 673, 720, 877, 903, 998
Bme1390I CCNGG 4 cut(s) 329, 385, 506, 953
Bme18I GGWCC 3 cut(s) 100, 472, 514
BmeRI GACNNNNNGTC 1 cut(s) 595
BmeT110I CYCGRG 2 cut(s) 414, 691
BmgBI CACGTC 2 cut(s) 18, 301
BmgT120I GGNCC 5 cut(s) 100, 472, 514, 1053, 1054
BmrFI CCNGG 4 cut(s) 329, 385, 506, 953
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 524
BmuI ACTGGG 1 cut(s) 82
BplI GAGNNNNNCTC 2 cut(s) 685, 717
BpmI CTGGAG 3 cut(s) 475, 796, 1308
Bpu14I TTCGAA 1 cut(s) 821
BpuEI CTTGAG 2 cut(s) 320, 883
Bsa29I ATCGAT 1 cut(s) 10
BsaBI GATNNNNATC 1 cut(s) 626
BsaHI GRCGYC 5 cut(s) 203, 591, 769, 878, 893
BsaI GGTCTC 3 cut(s) 338, 491, 514
BsaJI CCNNGG 5 cut(s) 327, 328, 384, 504, 652
BsaXI ACNNNNNCTCC 4 cut(s) 123, 153, 305, 335
Bsc4I CCNNNNNNNGG 7 cut(s) 181, 415, 469, 511, 728, 977, 1173
Bse118I RCCGGY 2 cut(s) 181, 771
Bse1I ACTGG 2 cut(s) 88, 1183
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 4 cut(s) 329, 385, 506, 953
BseCI ATCGAT 1 cut(s) 10
BseDI CCNNGG 5 cut(s) 327, 328, 384, 504, 652
BseGI GGATG 3 cut(s) 51, 107, 539
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 7 cut(s) 181, 415, 469, 511, 728, 977, 1173
BseNI ACTGG 2 cut(s) 88, 1183
BseRI GAGGAG 3 cut(s) 131, 144, 957
BseSI GKGCMC 2 cut(s) 300, 1057
BseXI GCAGC 2 cut(s) 705, 983
Bsh1236I CGCG 5 cut(s) 543, 674, 738, 780, 985
Bsh1285I CGRYCG 1 cut(s) 729
BshFI GGCC 3 cut(s) 230, 671, 1055
BshNI GGYRCC 4 cut(s) 459, 557, 732, 768
BshVI ATCGAT 1 cut(s) 10
BsiEI CGRYCG 1 cut(s) 729
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 2 cut(s) 414, 691
BsiSI CCGG 4 cut(s) 182, 631, 766, 772
BslFI GGGAC 2 cut(s) 190, 458
BslI CCNNNNNNNGG 7 cut(s) 181, 415, 469, 511, 728, 977, 1173
BsmAI GTCTC 7 cut(s) 276, 325, 338, 491, 514, 598, 884
BsmBI CGTCTC 4 cut(s) 276, 325, 598, 884
BsmFI GGGAC 2 cut(s) 190, 458
BsnI GGCC 3 cut(s) 230, 671, 1055
Bso31I GGTCTC 3 cut(s) 338, 491, 514
BsoBI CYCGRG 2 cut(s) 414, 691
Bsp119I TTCGAA 1 cut(s) 821
Bsp120I GGGCCC 1 cut(s) 1053
Bsp1286I GDGCHC 2 cut(s) 300, 1057
Bsp1407I TGTACA 1 cut(s) 1072
BspANI GGCC 3 cut(s) 230, 671, 1055
BspDI ATCGAT 1 cut(s) 10
BspFNI CGCG 5 cut(s) 543, 674, 738, 780, 985
BspMAI CTGCAG 1 cut(s) 709
BspPI GGATC 3 cut(s) 400, 616, 1044
BspT104I TTCGAA 1 cut(s) 821
BspT107I GGYRCC 4 cut(s) 459, 557, 732, 768
BspTNI GGTCTC 3 cut(s) 338, 491, 514
BsrBI CCGCTC 1 cut(s) 411
BsrFI RCCGGY 2 cut(s) 181, 771
BsrGI TGTACA 1 cut(s) 1072
BsrI ACTGG 2 cut(s) 88, 1183
BssAI RCCGGY 2 cut(s) 181, 771
BssECI CCNNGG 5 cut(s) 327, 328, 384, 504, 652
BssNI GRCGYC 5 cut(s) 203, 591, 769, 878, 893
Bst2UI CCWGG 4 cut(s) 329, 385, 506, 953
Bst4CI ACNGT 3 cut(s) 120, 562, 1266
Bst6I CTCTTC 2 cut(s) 248, 470
BstACI GRCGYC 5 cut(s) 203, 591, 769, 878, 893
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 1072
BstBI TTCGAA 1 cut(s) 821
BstC8I GCNNGC 6 cut(s) 187, 365, 716, 723, 773, 983
BstENI CCTNNNNNAGG 1 cut(s) 467
BstF5I GGATG 3 cut(s) 51, 107, 539
BstFNI CGCG 5 cut(s) 543, 674, 738, 780, 985
BstH2I RGCGCY 1 cut(s) 772
BstHHI GCGC 3 cut(s) 138, 196, 771
BstMAI GTCTC 7 cut(s) 276, 325, 338, 491, 514, 598, 884
BstMCI CGRYCG 1 cut(s) 729
BstMWI GCNNNNNNNGC 6 cut(s) 191, 768, 777, 792, 901, 1052
BstNI CCWGG 4 cut(s) 329, 385, 506, 953
BstNSI RCATGY 1 cut(s) 367
BstSCI CCNGG 4 cut(s) 327, 383, 504, 951
BstSFI CTRYAG 1 cut(s) 705
BstSLI GKGCMC 2 cut(s) 300, 1057
BstUI CGCG 5 cut(s) 543, 674, 738, 780, 985
BstV1I GCAGC 2 cut(s) 705, 983
BstX2I RGATCY 2 cut(s) 405, 519
BstYI RGATCY 2 cut(s) 405, 519
Bsu15I ATCGAT 1 cut(s) 10
BsuRI GGCC 3 cut(s) 230, 671, 1055
BsuTUI ATCGAT 1 cut(s) 10
BtrI CACGTC 2 cut(s) 18, 301
BtsCI GGATG 3 cut(s) 51, 107, 539
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 2 cut(s) 714, 837
Cac8I GCNNGC 6 cut(s) 187, 365, 716, 723, 773, 983
CfoI GCGC 3 cut(s) 138, 196, 771
Cfr10I RCCGGY 2 cut(s) 181, 771
Cfr13I GGNCC 5 cut(s) 100, 472, 514, 1053, 1054
ClaI ATCGAT 1 cut(s) 10
CseI GACGC 4 cut(s) 786, 867, 901, 1015
Csp6I GTAC 2 cut(s) 733, 1073
CspCI CAANNNNNGTGG 2 cut(s) 1151, 1186
CviAII CATG 6 cut(s) 39, 191, 364, 447, 787, 1225
CviQI GTAC 2 cut(s) 733, 1073
DinI GGCGCC 1 cut(s) 770
DriI GACNNNNNGTC 1 cut(s) 595
EaeI YGGCCR 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 248, 470
Eam1105I GACNNNNNGTC 1 cut(s) 595
EarI CTCTTC 2 cut(s) 248, 470
EciI GGCGGA 2 cut(s) 313, 385
Eco24I GRGCYC 1 cut(s) 1057
Eco31I GGTCTC 3 cut(s) 338, 491, 514
Eco47I GGWCC 3 cut(s) 100, 472, 514
Eco57I CTGAAG 1 cut(s) 929
Eco88I CYCGRG 2 cut(s) 414, 691
EcoNI CCTNNNNNAGG 1 cut(s) 467
EcoO109I RGGNCCY 3 cut(s) 100, 472, 1053
EcoRII CCWGG 4 cut(s) 327, 383, 504, 951
EcoT22I ATGCAT 1 cut(s) 1230
EcoT38I GRGCYC 1 cut(s) 1057
EgeI GGCGCC 1 cut(s) 770
EheI GGCGCC 1 cut(s) 770
Esp3I CGTCTC 4 cut(s) 276, 325, 598, 884
FaeI CATG 6 cut(s) 42, 194, 367, 450, 790, 1228
FaqI GGGAC 2 cut(s) 190, 458
FatI CATG 6 cut(s) 38, 190, 363, 446, 786, 1224
FauI CCCGC 3 cut(s) 111, 153, 931
FbaI TGATCA 2 cut(s) 573, 1125
FblI GTMKAC 2 cut(s) 390, 420
Fnu4HI GCNGC 6 cut(s) 361, 672, 719, 876, 902, 997
FokI GGATG 3 cut(s) 38, 94, 546
FriOI GRGCYC 1 cut(s) 1057
Fsp4HI GCNGC 6 cut(s) 361, 672, 719, 876, 902, 997
GlaI GCGC 3 cut(s) 137, 195, 770
GluI GCNGC 6 cut(s) 361, 672, 719, 876, 902, 997
GsuI CTGGAG 3 cut(s) 475, 796, 1308
HaeII RGCGCY 1 cut(s) 772
HaeIII GGCC 3 cut(s) 230, 671, 1055
HapII CCGG 4 cut(s) 182, 631, 766, 772
HgaI GACGC 4 cut(s) 786, 867, 901, 1015
HhaI GCGC 3 cut(s) 138, 196, 771
Hin1I GRCGYC 5 cut(s) 203, 591, 769, 878, 893
Hin1II CATG 6 cut(s) 42, 194, 367, 450, 790, 1228
Hin6I GCGC 3 cut(s) 136, 194, 769
HinP1I GCGC 3 cut(s) 136, 194, 769
HincII GTYRAC 2 cut(s) 391, 421
HindII GTYRAC 2 cut(s) 391, 421
HinfI GANTC 9 cut(s) 217, 392, 439, 483, 648, 694, 818, 842, 869
HpaII CCGG 4 cut(s) 182, 631, 766, 772
HphI GGTGA 5 cut(s) 343, 446, 539, 691, 876
Hpy166II GTNNAC 7 cut(s) 28, 298, 391, 421, 600, 615, 1176
Hpy188III TCNNGA 6 cut(s) 98, 268, 480, 517, 587, 642
Hpy8I GTNNAC 7 cut(s) 28, 298, 391, 421, 600, 615, 1176
HpyAV CCTTC 5 cut(s) 259, 648, 817, 904, 955
HpyCH4III ACNGT 3 cut(s) 120, 562, 1266
HpyCH4IV ACGT 6 cut(s) 17, 203, 292, 300, 591, 1063
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 768, 777, 792, 901, 1052
HpySE526I ACGT 6 cut(s) 17, 203, 292, 300, 591, 1063
Hsp92I GRCGYC 5 cut(s) 203, 591, 769, 878, 893
Hsp92II CATG 6 cut(s) 42, 194, 367, 450, 790, 1228
HspAI GCGC 3 cut(s) 136, 194, 769
KasI GGCGCC 1 cut(s) 768
KpnI GGTACC 1 cut(s) 736
KroI GCCGGC 1 cut(s) 771
KroNI GCCGGC 1 cut(s) 773
Ksp22I TGATCA 2 cut(s) 573, 1125
LmnI GCTCC 5 cut(s) 346, 706, 913, 970, 1289
Lsp1109I GCAGC 2 cut(s) 705, 983
LweI GCATC 1 cut(s) 524
MaeII ACGT 6 cut(s) 17, 203, 292, 300, 591, 1063
MaeIII GTNAC 2 cut(s) 349, 1306
MbiI CCGCTC 1 cut(s) 411
MboII GAAGA 4 cut(s) 235, 457, 657, 814
MfeI CAATTG 1 cut(s) 1102
MflI RGATCY 2 cut(s) 405, 519
MhlI GDGCHC 2 cut(s) 300, 1057
MluCI AATT 9 cut(s) 805, 932, 1017, 1067, 1094, 1102, 1130, 1278, 1293
Mly113I GGCGCC 1 cut(s) 769
MlyI GAGTC 2 cut(s) 386, 703
MmeI TCCRAC 4 cut(s) 467, 511, 995, 1014
Mph1103I ATGCAT 1 cut(s) 1230
MreI CGCCGGCG 1 cut(s) 771
MroNI GCCGGC 1 cut(s) 771
MseI TTAA 4 cut(s) 1020, 1209, 1238, 1342
MspI CCGG 4 cut(s) 182, 631, 766, 772
MspR9I CCNGG 4 cut(s) 329, 385, 506, 953
MunI CAATTG 1 cut(s) 1102
MvaI CCWGG 4 cut(s) 329, 385, 506, 953
MvnI CGCG 5 cut(s) 543, 674, 738, 780, 985
MwoI GCNNNNNNNGC 6 cut(s) 191, 768, 777, 792, 901, 1052
NaeI GCCGGC 1 cut(s) 773
NarI GGCGCC 1 cut(s) 769
NgoMIV GCCGGC 1 cut(s) 771
NlaIII CATG 6 cut(s) 42, 194, 367, 450, 790, 1228
NmuCI GTSAC 2 cut(s) 349, 1306
NsiI ATGCAT 1 cut(s) 1230
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 821
PaeI GCATGC 1 cut(s) 367
PaeR7I CTCGAG 1 cut(s) 691
PasI CCCWGGG 1 cut(s) 328
PcsI WCGNNNNNNNCGW 6 cut(s) 206, 285, 387, 558, 617, 735
PdiI GCCGGC 1 cut(s) 773
PfeI GAWTC 7 cut(s) 217, 439, 483, 648, 818, 842, 869
PflFI GACNNNGTC 1 cut(s) 202
PflMI CCANNNNNTGG 1 cut(s) 1173
PkrI GCNGC 6 cut(s) 362, 673, 720, 877, 903, 998
Ple19I CGATCG 1 cut(s) 729
PleI GAGTC 2 cut(s) 386, 702
PluTI GGCGCC 1 cut(s) 772
PpsI GAGTC 2 cut(s) 386, 702
PpuMI RGGWCCY 2 cut(s) 100, 472
PshBI ATTAAT 1 cut(s) 1238
Psp5II RGGWCCY 2 cut(s) 100, 472
Psp6I CCWGG 4 cut(s) 327, 383, 504, 951
PspGI CCWGG 4 cut(s) 327, 383, 504, 951
PspOMI GGGCCC 1 cut(s) 1053
PspPI GGNCC 5 cut(s) 100, 472, 514, 1053, 1054
PspPPI RGGWCCY 2 cut(s) 100, 472
PspXI VCTCGAGB 1 cut(s) 691
PstI CTGCAG 1 cut(s) 709
PsuI RGATCY 2 cut(s) 405, 519
PsyI GACNNNGTC 1 cut(s) 202
PvuI CGATCG 1 cut(s) 729
RsaI GTAC 2 cut(s) 734, 1074
RsaNI GTAC 2 cut(s) 733, 1073
SalI GTCGAC 2 cut(s) 389, 419
SaqAI TTAA 4 cut(s) 1020, 1209, 1238, 1342
SatI GCNGC 6 cut(s) 361, 672, 719, 876, 902, 997
Sau96I GGNCC 5 cut(s) 100, 472, 514, 1053, 1054
SchI GAGTC 2 cut(s) 386, 703
ScrFI CCNGG 4 cut(s) 329, 385, 506, 953
SduI GDGCHC 2 cut(s) 300, 1057
SfaNI GCATC 1 cut(s) 524
SfcI CTRYAG 1 cut(s) 705
SfoI GGCGCC 1 cut(s) 770
Sfr274I CTCGAG 1 cut(s) 691
SfuI TTCGAA 1 cut(s) 821
SgrAI CRCCGGYG 1 cut(s) 771
SinI GGWCC 3 cut(s) 100, 472, 514
SlaI CTCGAG 1 cut(s) 691
SmlI CTYRAG 3 cut(s) 335, 691, 862
SmoI CTYRAG 3 cut(s) 335, 691, 862
SphI GCATGC 1 cut(s) 367
Sse9I AATT 9 cut(s) 805, 932, 1017, 1067, 1094, 1102, 1130, 1278, 1293
SspDI GGCGCC 1 cut(s) 768
SspI AATATT 2 cut(s) 81, 1081
StyD4I CCNGG 4 cut(s) 327, 383, 504, 951
TaaI ACNGT 3 cut(s) 120, 562, 1266
TaiI ACGT 6 cut(s) 20, 206, 295, 303, 594, 1066
TaqII GACCGA 1 cut(s) 502
TasI AATT 9 cut(s) 805, 932, 1017, 1067, 1094, 1102, 1130, 1278, 1293
TatI WGTACW 1 cut(s) 1072
TauI GCSGC 4 cut(s) 363, 674, 878, 904
TfiI GAWTC 7 cut(s) 217, 439, 483, 648, 818, 842, 869
Tru1I TTAA 4 cut(s) 1020, 1209, 1238, 1342
Tru9I TTAA 4 cut(s) 1020, 1209, 1238, 1342
TscAI CASTG 2 cut(s) 714, 844
TseFI GTSAC 2 cut(s) 349, 1306
TseI GCWGC 2 cut(s) 718, 996
Tsp45I GTSAC 2 cut(s) 349, 1306
TspDTI ATGAA 3 cut(s) 24, 49, 1213
TspGWI ACGGA 6 cut(s) 224, 294, 337, 863, 989, 1020
TspRI CASTG 2 cut(s) 714, 844
Tth111I GACNNNGTC 1 cut(s) 202
Van91I CCANNNNNTGG 1 cut(s) 1173
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 3 cut(s) 100, 472, 514
VspI ATTAAT 1 cut(s) 1238
XagI CCTNNNNNAGG 1 cut(s) 467
XapI RAATTY 2 cut(s) 1067, 1130
XceI RCATGY 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 691
XmiI GTMKAC 2 cut(s) 390, 420
ZraI GACGTC 2 cut(s) 204, 592
Zsp2I ATGCAT 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.