pycom07g18670

Protein of unknown function, DUF547

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0004953
Physical Location & Seq
Forward (+)
21100209 .. 21100957
749 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 513 bp
ATGAAATTAGAAGACTTTCTAATGCAACGAAACGAGGAGGAGCAGAGAAGACTTGATCTTGAGGAAGAGGTTAAGAAATTGCAGGCGAAGTTGGAAGGAGAACAAACACTCGGCAGGTTTCTGCATTGTGCACTTCATGGACCTGTTCAGTCCCATCCACGTCTTTTGTCCTTGCTTCCAGCTCAGGTTCAGGAGCTGTTTTCGGAGATTGCAATGGTAGAGGAAGAGATAGGTCAACTTGACAGAAAAGTCGAAGAGCTGAAACTGAGGTTGTACAAAGAAAGGGAACAAACAAGAGAATGGAAAGTGCATATGTGGCAATTAGGGCAACAGAAGCAGTCGTTGTGTAGAGTGGGAAACCAGTCAGTGGTAAATGAAGAGAGATGCAGATCGGAAGCTTTGAGAAATTTGAGAAAAGAAAGAAGGTTGAAGGATAGAAGAGCATATGTGGGTTCTGCAGCAGATATCCCAAGGTGGAATTTCAGAGAGTCCGATGGTAAACTGCTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF547 Lzipper-MIP1

Protein Analysis

171

Amino Acids

20.26

Weight (kDa)

8.45

Isoelectric Point (pI)

59.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lzipper-MIP1 PF14389 13 - 94 2.3e-18 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
Acc36I ACCTGC 1 cut(s) 105
AccB7I CCANNNNNTGG 1 cut(s) 367
AcsI RAATTY 2 cut(s) 406, 478
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 1 cut(s) 430
AjiI CACGTC 1 cut(s) 161
AluBI AGCT 4 cut(s) 182, 196, 259, 398
AluI AGCT 4 cut(s) 182, 196, 259, 398
Alw21I GWGCWC 1 cut(s) 133
Alw44I GTGCAC 1 cut(s) 129
AlwNI CAGNNNCTG 1 cut(s) 196
ApaLI GTGCAC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 458
ApoI RAATTY 2 cut(s) 406, 478
Asp700I GAANNNNTTC 1 cut(s) 15
AspS9I GGNCC 1 cut(s) 140
AvaII GGWCC 1 cut(s) 140
BaeGI GKGCMC 1 cut(s) 133
BbsI GAAGAC 2 cut(s) 18, 55
Bbv12I GWGCWC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 470
BccI CCATC 2 cut(s) 162, 488
BfmI CTRYAG 1 cut(s) 456
BfuAI ACCTGC 1 cut(s) 105
BisI GCNGC 1 cut(s) 459
BlsI GCNGC 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 140
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 1 cut(s) 140
BmsI GCATC 1 cut(s) 374
BpiI GAAGAC 2 cut(s) 18, 55
Bpu10I CCTNAGC 1 cut(s) 183
BpuEI CTTGAG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 388
BsaJI CCNNGG 1 cut(s) 470
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 219
Bse8I GATNNNNATC 1 cut(s) 388
BseDI CCNNGG 1 cut(s) 470
BseGI GGATG 1 cut(s) 154
BseJI GATNNNNATC 1 cut(s) 388
BseLI CCNNNNNNNGG 1 cut(s) 367
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 197, 257
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 2 cut(s) 50, 53
BseSI GKGCMC 1 cut(s) 133
BseXI GCAGC 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 133
BslFI GGGAC 1 cut(s) 136
BslI CCNNNNNNNGG 1 cut(s) 367
BsmFI GGGAC 1 cut(s) 136
Bsp1286I GDGCHC 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 2 cut(s) 55, 389
BspCNI CTCAG 2 cut(s) 196, 258
BspMAI CTGCAG 1 cut(s) 460
BspMI ACCTGC 1 cut(s) 105
BspQI GCTCTTC 2 cut(s) 249, 433
BsrDI GCAATG 1 cut(s) 219
BsrGI TGTACA 1 cut(s) 273
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 470
BssMI GATC 2 cut(s) 55, 389
BssT1I CCWWGG 1 cut(s) 470
Bst6I CTCTTC 5 cut(s) 60, 219, 249, 372, 433
BstAUI TGTACA 1 cut(s) 273
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 183, 266
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 2 cut(s) 58, 392
BstMBI GATC 2 cut(s) 55, 389
BstMWI GCNNNNNNNGC 3 cut(s) 316, 325, 334
BstSFI CTRYAG 1 cut(s) 456
BstSLI GKGCMC 1 cut(s) 133
BstV1I GCAGC 1 cut(s) 470
BstV2I GAAGAC 2 cut(s) 18, 55
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 372
BveI ACCTGC 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 196
Cfr13I GGNCC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 274
CspCI CAANNNNNGTGG 2 cut(s) 147, 182
CviAII CATG 1 cut(s) 137
CviJI RGCY 4 cut(s) 182, 196, 259, 398
CviKI_1 RGCY 4 cut(s) 182, 196, 259, 398
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 2 cut(s) 183, 266
DpnI GATC 2 cut(s) 57, 391
DpnII GATC 2 cut(s) 55, 389
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 5 cut(s) 60, 219, 249, 372, 433
EarI CTCTTC 5 cut(s) 60, 219, 249, 372, 433
Eco130I CCWWGG 1 cut(s) 470
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 1 cut(s) 140
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 470
FaeI CATG 1 cut(s) 140
FaiI YATR 5 cut(s) 138, 312, 314, 445, 447
FaqI GGGAC 1 cut(s) 136
FatI CATG 1 cut(s) 136
FauNDI CATATG 2 cut(s) 312, 445
Fnu4HI GCNGC 1 cut(s) 459
FokI GGATG 1 cut(s) 141
Fsp4HI GCNGC 1 cut(s) 459
GluI GCNGC 1 cut(s) 459
Hin1II CATG 1 cut(s) 140
HincII GTYRAC 1 cut(s) 236
HindII GTYRAC 1 cut(s) 236
HindIII AAGCTT 1 cut(s) 396
HinfI GANTC 1 cut(s) 488
Hpy166II GTNNAC 3 cut(s) 131, 236, 500
Hpy188I TCNGA 4 cut(s) 205, 394, 485, 493
Hpy188III TCNNGA 2 cut(s) 59, 191
Hpy8I GTNNAC 3 cut(s) 131, 236, 500
HpyAV CCTTC 3 cut(s) 89, 417, 424
HpyCH4IV ACGT 1 cut(s) 160
HpyCH4V TGCA 8 cut(s) 25, 82, 124, 131, 212, 310, 387, 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 325, 334
HpyF3I CTNAG 2 cut(s) 183, 266
HpySE526I ACGT 1 cut(s) 160
Hsp92II CATG 1 cut(s) 140
Kzo9I GATC 2 cut(s) 55, 389
LguI GCTCTTC 2 cut(s) 249, 433
LmnI GCTCC 2 cut(s) 40, 193
LpnPI CCDG 7 cut(s) 68, 100, 156, 170, 176, 192, 374
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 1 cut(s) 374
MaeII ACGT 1 cut(s) 160
MalI GATC 2 cut(s) 57, 391
MboI GATC 2 cut(s) 55, 389
MboII GAAGA 7 cut(s) 23, 60, 77, 236, 266, 389, 450
MhlI GDGCHC 1 cut(s) 133
MluCI AATT 5 cut(s) 5, 77, 320, 406, 478
MlyI GAGTC 1 cut(s) 497
MmeI TCCRAC 1 cut(s) 72
MnlI CCTC 6 cut(s) 28, 31, 55, 61, 214, 261
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 2 cut(s) 72, 511
MwoI GCNNNNNNNGC 3 cut(s) 316, 325, 334
NdeI CATATG 2 cut(s) 312, 445
NdeII GATC 2 cut(s) 55, 389
NlaIII CATG 1 cut(s) 140
NmeAIII GCCGAG 1 cut(s) 90
PciSI GCTCTTC 2 cut(s) 249, 433
PdmI GAANNNNTTC 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 367
PkrI GCNGC 1 cut(s) 460
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
PspPI GGNCC 1 cut(s) 140
PstI CTGCAG 1 cut(s) 460
PstNI CAGNNNCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
SapI GCTCTTC 2 cut(s) 249, 433
SaqAI TTAA 2 cut(s) 72, 511
SatI GCNGC 1 cut(s) 459
Sau3AI GATC 2 cut(s) 55, 389
Sau96I GGNCC 1 cut(s) 140
SchI GAGTC 1 cut(s) 497
SduI GDGCHC 1 cut(s) 133
SfaNI GCATC 1 cut(s) 374
SfcI CTRYAG 1 cut(s) 456
SinI GGWCC 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
Sse9I AATT 5 cut(s) 5, 77, 320, 406, 478
StyI CCWWGG 1 cut(s) 470
TaiI ACGT 1 cut(s) 163
TaqI TCGA 1 cut(s) 252
TasI AATT 5 cut(s) 5, 77, 320, 406, 478
TatI WGTACW 1 cut(s) 273
Tru1I TTAA 2 cut(s) 72, 511
Tru9I TTAA 2 cut(s) 72, 511
TscAI CASTG 1 cut(s) 372
TseI GCWGC 1 cut(s) 458
TspDTI ATGAA 3 cut(s) 17, 125, 390
TspRI CASTG 1 cut(s) 372
Van91I CCANNNNNTGG 1 cut(s) 367
VneI GTGCAC 1 cut(s) 129
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 2 cut(s) 406, 478
XmnI GAANNNNTTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.