Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0004617

Physical Location & Seq

Genomic Coordinates Reverse (-)

21524747 .. 21528064

Gene Structure 3318 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g19210.7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGCAGACCGAGGCGAGAGTTGGGGTGGTGGTGGAAGGAGGGCAAAGAGCTCTGAATACCGGCCATGGAGGCGTCGCTGTTGAAGGTGGCGTTAGGACGCTCTCGCAGTCGCTGCCGCAGAAACAGCCGAAGCCGCTGCACCAACAGTCTCAGATCGGTGCGGTGTCACAGCTTCTCGCCGGAGGTGTCGCCGGCTCTCTCAGTAAGACTTGCACGGCACCTCTCGCTCGCCTCACTATTCTCTTTCAGGTGCAAGGTATGCACTCGGATGTTGCAACGATGCGAAAAGCTAGCATATGGCGTGAGGCCTCCAGAATTGCTAGTGAAGAAGGTTTTAGAGCTTTCTGGAAAGGAAATCTGGTGACAATTGCTCATCGTCTTCCTTATTCTTCTGTCAATTTCTATGCATACGAGCAATATAAAAAGTTGTTACAAAAAGTTCCAGGACTGGAAAATCATAGGGACAACTTGAGTACAGACCTTTGCGTGCATTTTGTGGGTGGAGGATCGGCGGGAATAACAGCTGCATCGGTTACATATCCTTTGGATCTTGTAAGGACGCGCCTTGCAGCTCAGACAAATGTGATGTACTACAAAGGAATTTGGCATACGCTGCGAACTATTACCCGGGATGAGGGTATTTTGGGTCTCTATAAGGGACTTGGAGCAACCCTCTTGGGTGTTGGGCCCAATATAGCTATCAGTTTTTCAGTGTATGAGACCTTGAGAGCTTCTTGGCAGTCACGTAGGCCCGAGGATTCTACTATGTTGGTTAGTCTTGCTTGTGGGAGCCTTTCAGGAATTGCATCATCAACAGCAACCTTTCCATTGGATCTTGTGAGGCGGCGGAAGCAGCTGGAAGGCGCTGGTGGCCGAGCCCGGGTCTACACAACAGGCCTTTTTGGCACATTTAAGCACATCTTCAAAACAGAAGGGTTGCGTGGCTTCTATAGGGGTATCCTACCAGAATACTACAAGGTGGTACCTGGTGTCGGTATCTGTTTCATGACGTACGAGACGTTGAAGGTGCTTTTAGCAGACGTCAGTGCTGCTATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0006810 GO:0006839 GO:0008150 GO:0016020 GO:0016021 GO:0019866 GO:0022857 GO:0031090 GO:0031224 GO:0031966 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0055085

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mito_carr

Protein Analysis

353

Amino Acids

38.17

Weight (kDa)

9.81

Isoelectric Point (pI)

40.18

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004617)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G55640 AT3G55640 AT3G55640 AT3G55640
fragaria_vesca	FvH4_7g23560 FvH4_7g23560 FvH4_7g23560 FvH4_7g23560
malus_domestica	MD01G1141400.v1.1 MD07G1205500.v1.1
prunus_persica	Prupe.2G241600_v2.0.a1 Prupe.2G241600_v2.0.a1 Prupe.2G241600_v2.0.a1 Prupe.2G241600_v2.0.a1 Prupe.2G241600_v2.0.a1 Prupe.2G241600_v2.0.a1 Prupe.2G241600_v2.0.a1
pyrus_communis	pycom01g16170 pycom07g19210
rosa_chinensis	RchiOBHm_Chr1g0368721
rosa_laevigata	RLG00000027194
rosa_multiflora	Rmu_sc0001791.1_g000016
rosa_roxburghii	Rroxscaffold_4G00288330
rosa_rugosa	Rorug01G0344200 Rorug01G0344200 Rorug01G0344200 Rorug01G0344300 Rorug01G0344400
rosa_samantha	Rh1AG351500 Rh1BG315200 Rh1DG346700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1044
Acc65I	GGTACC	1 cut(s)	982
AccB1I	GGYRCC	2 cut(s)	217, 982
AccI	GTMKAC	1 cut(s)	885
AccII	CGCG	1 cut(s)	562
AciI	CCGC	6 cut(s)	116, 134, 161, 512, 844, 847
AclWI	GGATC	3 cut(s)	514, 555, 840
AcoI	YGGCCR	2 cut(s)	61, 871
AcsI	RAATTY	1 cut(s)	600
AcyI	GRCGYC	2 cut(s)	72, 1041
AfaI	GTAC	4 cut(s)	475, 590, 984, 1013
AfiI	CCNNNNNNNGG	3 cut(s)	634, 880, 992
AgsI	TTSAA	3 cut(s)	83, 925, 1024
AjnI	CCWGG	2 cut(s)	442, 985
AluBI	AGCT	9 cut(s)	50, 172, 290, 341, 524, 572, 698, 731, 856
AluI	AGCT	9 cut(s)	50, 172, 290, 341, 524, 572, 698, 731, 856
Alw21I	GWGCWC	1 cut(s)	52
Alw26I	GTCTC	4 cut(s)	153, 653, 713, 1010
AlwI	GGATC	3 cut(s)	514, 555, 840
AlwNI	CAGNNNCTG	1 cut(s)	112
Ama87I	CYCGRG	3 cut(s)	627, 752, 879
AoxI	GGCC	6 cut(s)	61, 306, 686, 749, 871, 895
ApaI	GGGCCC	1 cut(s)	690
ApeKI	GCWGC	7 cut(s)	112, 136, 524, 569, 613, 853, 1049
ApoI	RAATTY	1 cut(s)	600
Asp718I	GGTACC	1 cut(s)	982
AspLEI	GCGC	2 cut(s)	564, 866
AspS9I	GGNCC	3 cut(s)	686, 687, 750
AsuC2I	CCSGG	4 cut(s)	628, 629, 880, 881
AsuHPI	GGTGA	1 cut(s)	373
AsuNHI	GCTAGC	1 cut(s)	290
AvaI	CYCGRG	3 cut(s)	627, 752, 879
BaeGI	GKGCMC	1 cut(s)	690
BanI	GGYRCC	2 cut(s)	217, 982
BanII	GRGCYC	3 cut(s)	52, 690, 880
BbsI	GAAGAC	1 cut(s)	371
Bbv12I	GWGCWC	1 cut(s)	52
BbvI	GCAGC	7 cut(s)	99, 123, 511, 581, 600, 865, 1036
BceAI	ACGGC	1 cut(s)	231
BcgI	CGANNNNNNTGC	2 cut(s)	118, 152
BciT130I	CCWGG	2 cut(s)	444, 987
BciVI	GTATCC	1 cut(s)	968
BcnI	CCSGG	4 cut(s)	628, 629, 880, 881
BcoDI	GTCTC	4 cut(s)	153, 653, 713, 1010
BfaI	CTAG	2 cut(s)	291, 321
BfmI	CTRYAG	1 cut(s)	949
BfoI	RGCGCY	1 cut(s)	867
BfuI	GTATCC	1 cut(s)	968
BglI	GCCNNNNNGGC	2 cut(s)	69, 903
Bme1390I	CCNGG	6 cut(s)	444, 628, 629, 880, 881, 987
BmeT110I	CYCGRG	3 cut(s)	627, 752, 879
BmgT120I	GGNCC	3 cut(s)	686, 687, 750
BmiI	GGNNCC	4 cut(s)	219, 688, 791, 984
BmrFI	CCNGG	6 cut(s)	444, 628, 629, 880, 881, 987
BmsI	GCATC	3 cut(s)	270, 536, 815
BmtI	GCTAGC	1 cut(s)	294
BpiI	GAAGAC	1 cut(s)	371
BplI	GAGNNNNNCTC	2 cut(s)	657, 689
BpmI	CTGGAG	1 cut(s)	295
BpuEI	CTTGAG	2 cut(s)	490, 745
BpuMI	CCSGG	4 cut(s)	628, 629, 880, 881
BsaAI	YACGTR	1 cut(s)	746
BsaHI	GRCGYC	2 cut(s)	72, 1041
BsaI	GGTCTC	2 cut(s)	653, 713
BsaJI	CCNNGG	5 cut(s)	9, 64, 627, 753, 879
Bsc4I	CCNNNNNNNGG	3 cut(s)	634, 880, 992
Bse118I	RCCGGY	2 cut(s)	59, 191
Bse1I	ACTGG	1 cut(s)	453
BseBI	CCWGG	2 cut(s)	444, 987
BseDI	CCNNGG	5 cut(s)	9, 64, 627, 753, 879
BseGI	GGATG	2 cut(s)	274, 637
BseLI	CCNNNNNNNGG	3 cut(s)	634, 880, 992
BseMII	CTCAG	3 cut(s)	164, 214, 587
BseNI	ACTGG	1 cut(s)	453
BseSI	GKGCMC	1 cut(s)	690
BseXI	GCAGC	7 cut(s)	99, 123, 511, 581, 600, 865, 1036
BsgI	GTGCAG	1 cut(s)	122
Bsh1236I	CGCG	1 cut(s)	562
BshFI	GGCC	6 cut(s)	63, 308, 688, 751, 873, 897
BshNI	GGYRCC	2 cut(s)	217, 982
BsiHKAI	GWGCWC	1 cut(s)	52
BsiHKCI	CYCGRG	3 cut(s)	627, 752, 879
BsiSI	CCGG	5 cut(s)	60, 180, 192, 628, 880
BsiWI	CGTACG	1 cut(s)	1011
BslFI	GGGAC	2 cut(s)	476, 672
BslI	CCNNNNNNNGG	3 cut(s)	634, 880, 992
BsmAI	GTCTC	4 cut(s)	153, 653, 713, 1010
BsmBI	CGTCTC	1 cut(s)	1010
BsmFI	GGGAC	2 cut(s)	476, 672
BsnI	GGCC	6 cut(s)	63, 308, 688, 751, 873, 897
Bso31I	GGTCTC	2 cut(s)	653, 713
BsoBI	CYCGRG	3 cut(s)	627, 752, 879
Bsp120I	GGGCCC	1 cut(s)	686
Bsp1286I	GDGCHC	3 cut(s)	52, 690, 880
Bsp143I	GATC	4 cut(s)	153, 506, 547, 832
Bsp19I	CCATGG	1 cut(s)	64
BspACI	CCGC	6 cut(s)	116, 134, 161, 512, 844, 847
BspANI	GGCC	6 cut(s)	63, 308, 688, 751, 873, 897
BspCNI	CTCAG	3 cut(s)	163, 213, 586
BspFNI	CGCG	1 cut(s)	562
BspHI	TCATGA	1 cut(s)	1005
BspLI	GGNNCC	4 cut(s)	219, 688, 791, 984
BspOI	GCTAGC	1 cut(s)	294
BspPI	GGATC	3 cut(s)	514, 555, 840
BspT107I	GGYRCC	2 cut(s)	217, 982
BspTNI	GGTCTC	2 cut(s)	653, 713
BsrFI	RCCGGY	2 cut(s)	59, 191
BsrI	ACTGG	1 cut(s)	453
BssAI	RCCGGY	2 cut(s)	59, 191
BssECI	CCNNGG	5 cut(s)	9, 64, 627, 753, 879
BssMI	GATC	4 cut(s)	153, 506, 547, 832
BssNI	GRCGYC	2 cut(s)	72, 1041
BssT1I	CCWWGG	1 cut(s)	64
Bst2UI	CCWGG	2 cut(s)	444, 987
Bst4CI	ACNGT	1 cut(s)	147
BstACI	GRCGYC	2 cut(s)	72, 1041
BstAPI	GCANNNNNTGC	3 cut(s)	112, 259, 613
BstBAI	YACGTR	1 cut(s)	746
BstC8I	GCNNGC	4 cut(s)	193, 229, 292, 488
BstDEI	CTNAG	3 cut(s)	150, 200, 573
BstDSI	CCRYGG	1 cut(s)	64
BstF5I	GGATG	2 cut(s)	274, 637
BstFNI	CGCG	1 cut(s)	562
BstH2I	RGCGCY	1 cut(s)	867
BstHHI	GCGC	2 cut(s)	564, 866
BstKTI	GATC	4 cut(s)	156, 509, 550, 835
BstMAI	GTCTC	4 cut(s)	153, 653, 713, 1010
BstMBI	GATC	4 cut(s)	153, 506, 547, 832
BstNI	CCWGG	2 cut(s)	444, 987
BstSCI	CCNGG	6 cut(s)	442, 626, 627, 878, 879, 985
BstSFI	CTRYAG	1 cut(s)	949
BstSLI	GKGCMC	1 cut(s)	690
BstUI	CGCG	1 cut(s)	562
BstV1I	GCAGC	7 cut(s)	99, 123, 511, 581, 600, 865, 1036
BstV2I	GAAGAC	1 cut(s)	371
BstX2I	RGATCY	2 cut(s)	547, 832
BstYI	RGATCY	2 cut(s)	547, 832
BsuI	GTATCC	1 cut(s)	968
BsuRI	GGCC	6 cut(s)	63, 308, 688, 751, 873, 897
BtgI	CCRYGG	1 cut(s)	64
BtsCI	GGATG	2 cut(s)	274, 637
BtsIMutI	CAGTG	2 cut(s)	717, 1051
Cac8I	GCNNGC	4 cut(s)	193, 229, 292, 488
CaiI	CAGNNNCTG	1 cut(s)	112
CciI	TCATGA	1 cut(s)	1005
CfoI	GCGC	2 cut(s)	564, 866
Cfr10I	RCCGGY	2 cut(s)	59, 191
Cfr13I	GGNCC	3 cut(s)	686, 687, 750
Cfr9I	CCCGGG	2 cut(s)	627, 879
CseI	GACGC	3 cut(s)	61, 106, 568
CsiI	ACCWGGT	1 cut(s)	985
Csp6I	GTAC	4 cut(s)	474, 589, 983, 1012
CviAII	CATG	2 cut(s)	65, 1006
CviQI	GTAC	4 cut(s)	474, 589, 983, 1012
DdeI	CTNAG	3 cut(s)	150, 200, 573
DpnI	GATC	4 cut(s)	155, 508, 549, 834
DpnII	GATC	4 cut(s)	153, 506, 547, 832
EaeI	YGGCCR	2 cut(s)	61, 871
EciI	GGCGGA	1 cut(s)	862
Ecl136II	GAGCTC	1 cut(s)	50
Eco130I	CCWWGG	1 cut(s)	64
Eco147I	AGGCCT	2 cut(s)	308, 897
Eco24I	GRGCYC	3 cut(s)	52, 690, 880
Eco31I	GGTCTC	2 cut(s)	653, 713
Eco53kI	GAGCTC	1 cut(s)	50
Eco88I	CYCGRG	3 cut(s)	627, 752, 879
EcoICRI	GAGCTC	1 cut(s)	50
EcoRII	CCWGG	2 cut(s)	442, 985
EcoT14I	CCWWGG	1 cut(s)	64
EcoT22I	ATGCAT	1 cut(s)	409
EcoT38I	GRGCYC	3 cut(s)	52, 690, 880
ErhI	CCWWGG	1 cut(s)	64
Esp3I	CGTCTC	1 cut(s)	1010
FaeI	CATG	2 cut(s)	68, 1009
FalI	AAGNNNNNCTT	2 cut(s)	905, 937
FaqI	GGGAC	2 cut(s)	476, 672
FatI	CATG	2 cut(s)	64, 1005
FauI	CCCGC	1 cut(s)	505
FauNDI	CATATG	1 cut(s)	296
FblI	GTMKAC	1 cut(s)	885
FokI	GGATG	2 cut(s)	281, 644
FriOI	GRGCYC	3 cut(s)	52, 690, 880
FspBI	CTAG	2 cut(s)	291, 321
GlaI	GCGC	2 cut(s)	563, 865
GsuI	CTGGAG	1 cut(s)	295
HaeII	RGCGCY	1 cut(s)	867
HaeIII	GGCC	6 cut(s)	63, 308, 688, 751, 873, 897
HapII	CCGG	5 cut(s)	60, 180, 192, 628, 880
HgaI	GACGC	3 cut(s)	61, 106, 568
HhaI	GCGC	2 cut(s)	564, 866
Hin1I	GRCGYC	2 cut(s)	72, 1041
Hin1II	CATG	2 cut(s)	68, 1009
Hin6I	GCGC	2 cut(s)	562, 864
HinP1I	GCGC	2 cut(s)	562, 864
HinfI	GANTC	1 cut(s)	758
HpaII	CCGG	5 cut(s)	60, 180, 192, 628, 880
HphI	GGTGA	1 cut(s)	373
Hpy166II	GTNNAC	1 cut(s)	886
Hpy188I	TCNGA	4 cut(s)	54, 153, 268, 576
Hpy188III	TCNNGA	4 cut(s)	312, 346, 798, 1006
Hpy8I	GTNNAC	1 cut(s)	886
Hpy99I	CGWCG	1 cut(s)	77
HpyAV	CCTTC	6 cut(s)	29, 77, 323, 854, 926, 1018
HpyCH4III	ACNGT	1 cut(s)	147
HpyCH4IV	ACGT	4 cut(s)	745, 1010, 1019, 1041
HpyF3I	CTNAG	3 cut(s)	150, 200, 573
HpySE526I	ACGT	4 cut(s)	745, 1010, 1019, 1041
Hsp92I	GRCGYC	2 cut(s)	72, 1041
Hsp92II	CATG	2 cut(s)	68, 1009
HspAI	GCGC	2 cut(s)	562, 864
KpnI	GGTACC	1 cut(s)	986
KroI	GCCGGC	1 cut(s)	191
KroNI	GCCGGC	1 cut(s)	193
Kzo9I	GATC	4 cut(s)	153, 506, 547, 832
LmnI	GCTCC	2 cut(s)	665, 789
Lsp1109I	GCAGC	7 cut(s)	99, 123, 511, 581, 600, 865, 1036
LweI	GCATC	3 cut(s)	270, 536, 815
MabI	ACCWGGT	1 cut(s)	985
MaeI	CTAG	2 cut(s)	291, 321
MaeII	ACGT	4 cut(s)	745, 1010, 1019, 1041
MaeIII	GTNAC	5 cut(s)	165, 361, 429, 532, 741
MalI	GATC	4 cut(s)	155, 508, 549, 834
MboI	GATC	4 cut(s)	153, 506, 547, 832
MboII	GAAGA	4 cut(s)	338, 371, 381, 913
MfeI	CAATTG	1 cut(s)	366
MflI	RGATCY	2 cut(s)	547, 832
MhlI	GDGCHC	3 cut(s)	52, 690, 880
MluCI	AATT	5 cut(s)	315, 366, 397, 600, 801
Mph1103I	ATGCAT	1 cut(s)	409
MroNI	GCCGGC	1 cut(s)	191
MseI	TTAA	1 cut(s)	912
MslI	CAYNNNNRTG	1 cut(s)	267
MspA1I	CMGCKG	3 cut(s)	136, 524, 856
MspI	CCGG	5 cut(s)	60, 180, 192, 628, 880
MspR9I	CCNGG	6 cut(s)	444, 628, 629, 880, 881, 987
MunI	CAATTG	1 cut(s)	366
MvaI	CCWGG	2 cut(s)	444, 987
MvnI	CGCG	1 cut(s)	562
NaeI	GCCGGC	1 cut(s)	193
NciI	CCSGG	4 cut(s)	628, 629, 880, 881
NcoI	CCATGG	1 cut(s)	64
NdeI	CATATG	1 cut(s)	296
NdeII	GATC	4 cut(s)	153, 506, 547, 832
NgoMIV	GCCGGC	1 cut(s)	191
NheI	GCTAGC	1 cut(s)	290
NlaIII	CATG	2 cut(s)	68, 1009
NlaIV	GGNNCC	4 cut(s)	219, 688, 791, 984
NmeAIII	GCCGAG	1 cut(s)	899
NmuCI	GTSAC	3 cut(s)	165, 361, 741
NsiI	ATGCAT	1 cut(s)	409
PagI	TCATGA	1 cut(s)	1005
PceI	AGGCCT	2 cut(s)	308, 897
PcsI	WCGNNNNNNNCGW	1 cut(s)	1016
PdiI	GCCGGC	1 cut(s)	193
PfeI	GAWTC	1 cut(s)	758
Pfl23II	CGTACG	1 cut(s)	1011
PfoI	TCCNGGA	1 cut(s)	442
Ppu21I	YACGTR	1 cut(s)	746
Psp124BI	GAGCTC	1 cut(s)	52
Psp6I	CCWGG	2 cut(s)	442, 985
PspGI	CCWGG	2 cut(s)	442, 985
PspLI	CGTACG	1 cut(s)	1011
PspN4I	GGNNCC	4 cut(s)	219, 688, 791, 984
PspOMI	GGGCCC	1 cut(s)	686
PspPI	GGNCC	3 cut(s)	686, 687, 750
PstNI	CAGNNNCTG	1 cut(s)	112
PsuI	RGATCY	2 cut(s)	547, 832
PvuII	CAGCTG	2 cut(s)	524, 856
RsaI	GTAC	4 cut(s)	475, 590, 984, 1013
RsaNI	GTAC	4 cut(s)	474, 589, 983, 1012
RseI	CAYNNNNRTG	1 cut(s)	267
SacI	GAGCTC	1 cut(s)	52
SaqAI	TTAA	1 cut(s)	912
Sau3AI	GATC	4 cut(s)	153, 506, 547, 832
Sau96I	GGNCC	3 cut(s)	686, 687, 750
ScrFI	CCNGG	6 cut(s)	444, 628, 629, 880, 881, 987
SduI	GDGCHC	3 cut(s)	52, 690, 880
SexAI	ACCWGGT	1 cut(s)	985
SfaNI	GCATC	3 cut(s)	270, 536, 815
SfcI	CTRYAG	1 cut(s)	949
SmaI	CCCGGG	2 cut(s)	629, 881
SmiMI	CAYNNNNRTG	1 cut(s)	267
SmlI	CTYRAG	2 cut(s)	469, 724
SmoI	CTYRAG	2 cut(s)	469, 724
Sse9I	AATT	5 cut(s)	315, 366, 397, 600, 801
SseBI	AGGCCT	2 cut(s)	308, 897
SsiI	CCGC	6 cut(s)	116, 134, 161, 512, 844, 847
SspMI	CTAG	2 cut(s)	291, 321
SstI	GAGCTC	1 cut(s)	52
StuI	AGGCCT	2 cut(s)	308, 897
StyD4I	CCNGG	6 cut(s)	442, 626, 627, 878, 879, 985
StyI	CCWWGG	1 cut(s)	64
TaaI	ACNGT	1 cut(s)	147
TaiI	ACGT	4 cut(s)	748, 1013, 1022, 1044
TaqII	GACCGA	1 cut(s)	23
TasI	AATT	5 cut(s)	315, 366, 397, 600, 801
TatI	WGTACW	2 cut(s)	473, 588
TauI	GCSGC	3 cut(s)	118, 136, 847
TfiI	GAWTC	1 cut(s)	758
Tru1I	TTAA	1 cut(s)	912
Tru9I	TTAA	1 cut(s)	912
TscAI	CASTG	2 cut(s)	717, 1051
TseFI	GTSAC	3 cut(s)	165, 361, 741
TseI	GCWGC	7 cut(s)	112, 136, 524, 569, 613, 853, 1049
Tsp45I	GTSAC	3 cut(s)	165, 361, 741
TspDTI	ATGAA	1 cut(s)	994
TspMI	CCCGGG	2 cut(s)	627, 879
TspRI	CASTG	2 cut(s)	717, 1051
XapI	RAATTY	1 cut(s)	600
XmaI	CCCGGG	2 cut(s)	627, 879
XmiI	GTMKAC	1 cut(s)	885
XspI	CTAG	2 cut(s)	291, 321
ZraI	GACGTC	1 cut(s)	1042
Zsp2I	ATGCAT	1 cut(s)	409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.