pycom07g21220
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010870
Physical Location & Seq
Forward (+)
23035560 .. 23037706
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 897 bp
ATGGATGAATTCATTTCTTTAATCCTCCATGGATGCGAGTTGGCTAGAGAGCTTGGAAAAGACCTACCCAACTTGGCCACCCAACCTAACTCACTCTTCAATTCATTGGAAGAAATCACAAAGATTTTTGGTTCAGCAAAAGAAAGGGTTTTACATAGTCAAGATTCAAGTAGTAGTTCATATCTTCACAACACTTTAACACTTGAGCACCAGCAGATTGGAACAAGTGTTGAACAATGGCTGAGGCGTAATCCGCAGGCAATGGATATAATCCAAACCCAACTAGCAGCTGATCGATACGAAGCGAAGATCAGTGGTGGCATTGATATCCGGGGTTCGGAGGCGGCAGTTATGGGTTCTCAGGCAGTGGATGTTGTGCCAGCTTCTGACGTGAACATTGCTTCATCAAGCTCACAAAGACCGCGACAAAGGAGTTACTATAGATGCACCCATCAAAAGTTGTACAATTGCCTGGCCAAGAAGCTTGTCCAGAGGCTAGACGATGATCCCTCGACATTTGAAGTAACGTACAGAGGTGAACACACATGCCACATGTCAGCCACTGCACCTTCAATTCTACCACCCGCAGCTGATCATCATATTACACAAGATCAGATGGCAGCAACCACTGAGTCTCCAACATCTCGATGGCTTTCCATGGACTTTAACCCTATTAGAGGGGGCAGCAGCAGCGGTGGTATGATAGGTGGCAGTTGTGGCGGCGGTGGTGATGACGTCGGTACATCCACCGGCACACGTTACACCGGTAAAGAAGTTGACTTTCCTGTGATGGATTTGGCTGATGCTATGTTTAATTCAGGCAGCAGCAGCAGCAGTATGGATTTTATTTTCAATTCTGCTGAACGTAGATGGGAGCCAGATGACAAGAAACATTGA

Protein Analysis

299

Amino Acids

32.58

Weight (kDa)

5.34

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 140 - 184 3.7e-14 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010870)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40740 AT2G40740 AT2G40740 AT3G56390
fragaria_vesca FvH4_7g26020
malus_domestica MD01G1168500.v1.1 MD07G1234600.v1.1
prunus_persica Prupe.2G264900_v2.0.a1
pyrus_communis pycom01g18150 pycom07g21220
rosa_chinensis RchiOBHm_Chr1g0372431
rosa_laevigata RLG00000026899
rosa_multiflora Rmu_sc0001755.1_g000002
rosa_roxburghii Rroxscaffold_4G00284930
rosa_rugosa Rorug01G0371800
rosa_samantha Rh1AG380300 Rh1BG343900 Rh1CG357300 Rh1DG375100
rosa_wichuraiana Rw1G033440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 738
AccII CGCG 1 cut(s) 424
AciI CCGC 7 cut(s) 254, 344, 422, 585, 693, 720, 723
AclWI GGATC 1 cut(s) 500
AcoI YGGCCR 2 cut(s) 75, 474
AcsI RAATTY 1 cut(s) 8
AcyI GRCGYC 1 cut(s) 735
AfaI GTAC 3 cut(s) 464, 530, 742
AfiI CCNNNNNNNGG 2 cut(s) 337, 677
AflIII ACRYGT 2 cut(s) 552, 755
AgeI ACCGGT 1 cut(s) 764
AgsI TTSAA 6 cut(s) 100, 168, 233, 521, 573, 853
AjiI CACGTC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 471
AluBI AGCT 6 cut(s) 52, 290, 383, 411, 484, 590
AluI AGCT 6 cut(s) 52, 290, 383, 411, 484, 590
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 1 cut(s) 500
AlwNI CAGNNNCTG 2 cut(s) 386, 563
AoxI GGCC 2 cut(s) 75, 474
ApoI RAATTY 1 cut(s) 8
AsiGI ACCGGT 1 cut(s) 764
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 548, 740
BalI TGGCCA 2 cut(s) 77, 476
Bbv12I GWGCWC 1 cut(s) 210
BbvCI CCTCAGC 1 cut(s) 242
BccI CCATC 5 cut(s) 459, 610, 642, 784, 864
BciT130I CCWGG 1 cut(s) 473
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 3 cut(s) 45, 284, 497
BfmI CTRYAG 1 cut(s) 439
Bme1390I CCNGG 2 cut(s) 332, 473
BmgBI CACGTC 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 876
BmrFI CCNGG 2 cut(s) 332, 473
BmsI GCATC 3 cut(s) 23, 434, 793
Bpu10I CCTNAGC 1 cut(s) 242
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 332
Bsa29I ATCGAT 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 3 cut(s) 28, 331, 657
BsaWI WCCGGW 1 cut(s) 764
BsaXI ACNNNNNCTCC 2 cut(s) 619, 649
Bsc4I CCNNNNNNNGG 2 cut(s) 337, 677
Bse118I RCCGGY 2 cut(s) 749, 764
Bse3DI GCAATG 2 cut(s) 267, 396
BseBI CCWGG 1 cut(s) 473
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 3 cut(s) 28, 331, 657
BseGI GGATG 4 cut(s) 10, 38, 376, 743
BseLI CCNNNNNNNGG 2 cut(s) 337, 677
BseMI GCAATG 2 cut(s) 267, 396
BseMII CTCAG 3 cut(s) 233, 374, 621
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 424
BshFI GGCC 2 cut(s) 77, 476
BshTI ACCGGT 1 cut(s) 764
BshVI ATCGAT 1 cut(s) 295
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 3 cut(s) 331, 750, 765
BslI CCNNNNNNNGG 2 cut(s) 337, 677
BsmAI GTCTC 1 cut(s) 639
BsnI GGCC 2 cut(s) 77, 476
Bsp1286I GDGCHC 1 cut(s) 210
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 5 cut(s) 292, 309, 505, 592, 610
Bsp19I CCATGG 2 cut(s) 28, 657
BspACI CCGC 7 cut(s) 254, 344, 422, 585, 693, 720, 723
BspANI GGCC 2 cut(s) 77, 476
BspCNI CTCAG 3 cut(s) 234, 373, 622
BspDI ATCGAT 1 cut(s) 295
BspFNI CGCG 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 876
BspPI GGATC 1 cut(s) 500
BsrDI GCAATG 2 cut(s) 267, 396
BsrFI RCCGGY 2 cut(s) 749, 764
BsrGI TGTACA 1 cut(s) 462
BssAI RCCGGY 2 cut(s) 749, 764
BssECI CCNNGG 3 cut(s) 28, 331, 657
BssMI GATC 5 cut(s) 292, 309, 505, 592, 610
BssNI GRCGYC 1 cut(s) 735
BssT1I CCWWGG 2 cut(s) 28, 657
Bst2UI CCWGG 1 cut(s) 473
Bst6I CTCTTC 1 cut(s) 101
BstACI GRCGYC 1 cut(s) 735
BstAUI TGTACA 1 cut(s) 462
BstC8I GCNNGC 2 cut(s) 258, 381
BstDEI CTNAG 3 cut(s) 242, 360, 630
BstDSI CCRYGG 2 cut(s) 28, 657
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 4 cut(s) 10, 38, 376, 743
BstFNI CGCG 1 cut(s) 424
BstKTI GATC 5 cut(s) 295, 312, 508, 595, 613
BstMAI GTCTC 1 cut(s) 639
BstMBI GATC 5 cut(s) 292, 309, 505, 592, 610
BstMWI GCNNNNNNNGC 5 cut(s) 253, 690, 717, 828, 831
BstNI CCWGG 1 cut(s) 473
BstNSI RCATGY 2 cut(s) 549, 556
BstSCI CCNGG 2 cut(s) 330, 471
BstSFI CTRYAG 1 cut(s) 439
BstUI CGCG 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 218
Bsu15I ATCGAT 1 cut(s) 295
BsuRI GGCC 2 cut(s) 77, 476
BsuTUI ATCGAT 1 cut(s) 295
BtgI CCRYGG 2 cut(s) 28, 657
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 4 cut(s) 10, 38, 376, 743
BtsI GCAGTG 2 cut(s) 372, 561
BtsIMutI CAGTG 4 cut(s) 319, 372, 561, 627
Cac8I GCNNGC 2 cut(s) 258, 381
CaiI CAGNNNCTG 2 cut(s) 386, 563
Cfr10I RCCGGY 2 cut(s) 749, 764
ClaI ATCGAT 1 cut(s) 295
Csp6I GTAC 3 cut(s) 463, 529, 741
CspAI ACCGGT 1 cut(s) 764
CviAII CATG 4 cut(s) 29, 546, 553, 658
CviQI GTAC 3 cut(s) 463, 529, 741
DdeI CTNAG 3 cut(s) 242, 360, 630
DpnI GATC 5 cut(s) 294, 311, 507, 594, 612
DpnII GATC 5 cut(s) 292, 309, 505, 592, 610
EaeI YGGCCR 2 cut(s) 75, 474
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 28, 657
Eco32I GATATC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 471
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 28, 657
ErhI CCWWGG 2 cut(s) 28, 657
FaeI CATG 4 cut(s) 32, 549, 556, 661
FatI CATG 4 cut(s) 28, 545, 552, 657
FauI CCCGC 1 cut(s) 592
FbaI TGATCA 1 cut(s) 592
FokI GGATG 4 cut(s) 17, 45, 383, 730
FspBI CTAG 3 cut(s) 45, 284, 497
HaeIII GGCC 2 cut(s) 77, 476
HapII CCGG 3 cut(s) 331, 750, 765
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 4 cut(s) 32, 549, 556, 661
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 2 cut(s) 164, 632
HpaII CCGG 3 cut(s) 331, 750, 765
HphI GGTGA 2 cut(s) 548, 740
Hpy166II GTNNAC 3 cut(s) 394, 539, 778
Hpy188I TCNGA 3 cut(s) 340, 388, 615
Hpy188III TCNNGA 3 cut(s) 161, 490, 645
Hpy8I GTNNAC 3 cut(s) 394, 539, 778
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 1 cut(s) 579
HpyCH4IV ACGT 5 cut(s) 390, 527, 735, 757, 865
HpyCH4V TGCA 2 cut(s) 447, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 253, 690, 717, 828, 831
HpyF3I CTNAG 3 cut(s) 242, 360, 630
HpySE526I ACGT 5 cut(s) 390, 527, 735, 757, 865
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 4 cut(s) 32, 549, 556, 661
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 5 cut(s) 292, 309, 505, 592, 610
LmnI GCTCC 1 cut(s) 874
LweI GCATC 3 cut(s) 23, 434, 793
MaeI CTAG 3 cut(s) 45, 284, 497
MaeII ACGT 5 cut(s) 390, 527, 735, 757, 865
MaeIII GTNAC 3 cut(s) 434, 523, 758
MalI GATC 5 cut(s) 294, 311, 507, 594, 612
MboI GATC 5 cut(s) 292, 309, 505, 592, 610
MboII GAAGA 4 cut(s) 88, 122, 176, 319
MfeI CAATTG 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 2 cut(s) 77, 476
MluCI AATT 6 cut(s) 8, 100, 466, 573, 814, 853
MluNI TGGCCA 2 cut(s) 77, 476
MlyI GAGTC 1 cut(s) 641
MmeI TCCRAC 1 cut(s) 662
MnlI CCTC 7 cut(s) 35, 237, 334, 486, 520, 527, 671
Mox20I TGGCCA 2 cut(s) 77, 476
MscI TGGCCA 2 cut(s) 77, 476
MseI TTAA 4 cut(s) 20, 197, 666, 813
MslI CAYNNNNRTG 1 cut(s) 646
Msp20I TGGCCA 2 cut(s) 77, 476
MspA1I CMGCKG 3 cut(s) 290, 590, 693
MspI CCGG 3 cut(s) 331, 750, 765
MspR9I CCNGG 2 cut(s) 332, 473
MunI CAATTG 1 cut(s) 466
MvaI CCWGG 1 cut(s) 473
MvnI CGCG 1 cut(s) 424
MwoI GCNNNNNNNGC 5 cut(s) 253, 690, 717, 828, 831
NciI CCSGG 1 cut(s) 332
NcoI CCATGG 2 cut(s) 28, 657
NdeII GATC 5 cut(s) 292, 309, 505, 592, 610
NlaIII CATG 4 cut(s) 32, 549, 556, 661
NlaIV GGNNCC 1 cut(s) 876
NspI RCATGY 2 cut(s) 549, 556
PciI ACATGT 1 cut(s) 552
PfeI GAWTC 1 cut(s) 164
PinAI ACCGGT 1 cut(s) 764
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
PscI ACATGT 1 cut(s) 552
Psp6I CCWGG 1 cut(s) 471
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 876
PstNI CAGNNNCTG 2 cut(s) 386, 563
PvuII CAGCTG 2 cut(s) 290, 590
RsaI GTAC 3 cut(s) 464, 530, 742
RsaNI GTAC 3 cut(s) 463, 529, 741
RseI CAYNNNNRTG 1 cut(s) 646
SaqAI TTAA 4 cut(s) 20, 197, 666, 813
Sau3AI GATC 5 cut(s) 292, 309, 505, 592, 610
SchI GAGTC 1 cut(s) 641
ScrFI CCNGG 2 cut(s) 332, 473
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 3 cut(s) 23, 434, 793
SfcI CTRYAG 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 646
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 6 cut(s) 8, 100, 466, 573, 814, 853
SsiI CCGC 7 cut(s) 254, 344, 422, 585, 693, 720, 723
SspMI CTAG 3 cut(s) 45, 284, 497
StyD4I CCNGG 2 cut(s) 330, 471
StyI CCWWGG 2 cut(s) 28, 657
TaiI ACGT 5 cut(s) 393, 530, 738, 760, 868
TaqI TCGA 3 cut(s) 295, 512, 646
TasI AATT 6 cut(s) 8, 100, 466, 573, 814, 853
TatI WGTACW 1 cut(s) 462
TauI GCSGC 2 cut(s) 347, 723
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 4 cut(s) 20, 197, 666, 813
Tru9I TTAA 4 cut(s) 20, 197, 666, 813
TscAI CASTG 4 cut(s) 319, 372, 568, 634
TspDTI ATGAA 4 cut(s) 21, 93, 168, 393
TspRI CASTG 4 cut(s) 319, 372, 568, 634
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 1 cut(s) 8
XceI RCATGY 2 cut(s) 549, 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 645
XspI CTAG 3 cut(s) 45, 284, 497
ZraI GACGTC 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.