pycom07g21300

aarF domain-containing protein kinase At5g05200

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006647
Physical Location & Seq
Forward (+)
23068546 .. 23070118
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 741 bp
ATGGGACTCACAAGGCAGGCTACGGCTCCAAAAGTGTATCACCAATGCAGCAGCAAACGAGTTTTAACAATGGAGAGGCTGTACGGAGTTCCTCTTACTGACTTAAACAGTATAAGTTCCTTTGTCTCTAATCCGGAGACCAGTCTCATAACTGCCCTTAATGTGTGGTTTGGTAGTTTGCTTGCCTGTGAAAGCTTCCACGCAGATGTGCATGCAGGGAACCTGTGGTTGTTGCGCGATGGCCGCATTGGGTTTCTTGATTTTGGAATTGTTGGCCGTATATCCCCAAAAACATGGGGTGCCATGGAAATCTTTTTGGCATCAATTGCAGCTGAAGAATATGAATCAATGGCATCTGCGCTGATTGAAATGGGTGCTACAGACACAAATGTTGATACTAAGGCCTTTGCAAGAGACTTGGAAAAGATGTTCTCATCAATAAAGGATTTGGATACTGAAGTAGTCATAGCAACTGCCAGGGAGCCATCTACGAATGCAACTGCTGTCTCTGCTAATCTAGTTGTTGATGAGAGACAAATGAATGCACTTTTTCTCGATGTGATTCGGGTTAGTGAATCATATGGATTGAAATTTCCACGAGAGTTTGCACTTCTCCTGAAGCAGCTTTTGTATTTTGATCGATACACCCGTTTGTTGGCCCCGAACTTGAATATGCTCCAAGACCAAAGGATTTCCATCGCTTCTAATCGAAGAACCAACTACAGGAACAATTTCAGATGA

Protein Analysis

247

Amino Acids

27.62

Weight (kDa)

6.33

Isoelectric Point (pI)

28.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC1 PF03109 6 - 119 7.3e-21 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 299
AccII CGCG 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 244
AcoI YGGCCR 2 cut(s) 241, 274
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 3 cut(s) 354, 477, 638
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 655, 723
AgsI TTSAA 3 cut(s) 368, 589, 670
AjnI CCWGG 1 cut(s) 476
AloI GAACNNNNNNTCC 2 cut(s) 413, 445
AluBI AGCT 3 cut(s) 195, 332, 625
AluI AGCT 3 cut(s) 195, 332, 625
Alw26I GTCTC 6 cut(s) 130, 131, 149, 408, 511, 526
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 4 cut(s) 241, 274, 402, 657
ApeKI GCWGC 4 cut(s) 48, 51, 329, 622
ApoI RAATTY 1 cut(s) 590
Asp700I GAANNNNTTC 1 cut(s) 731
AspLEI GCGC 2 cut(s) 237, 361
AspS9I GGNCC 1 cut(s) 658
AsuHPI GGTGA 1 cut(s) 32
BaeI ACNNNNGTAYC 4 cut(s) 444, 477, 634, 667
BanI GGYRCC 1 cut(s) 299
BauI CACGAG 1 cut(s) 597
BbvI GCAGC 4 cut(s) 60, 63, 341, 634
BccI CCATC 3 cut(s) 233, 493, 704
BceAI ACGGC 2 cut(s) 39, 261
BciT130I CCWGG 1 cut(s) 478
BciVI GTATCC 1 cut(s) 445
BcoDI GTCTC 6 cut(s) 130, 131, 149, 408, 511, 526
BfaI CTAG 1 cut(s) 518
BfmI CTRYAG 2 cut(s) 378, 721
BfuI GTATCC 1 cut(s) 445
BisI GCNGC 5 cut(s) 49, 52, 244, 330, 623
BlsI GCNGC 5 cut(s) 50, 53, 245, 331, 624
Bme1390I CCNGG 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 658
BmiI GGNNCC 5 cut(s) 27, 221, 301, 483, 660
BmrFI CCNGG 1 cut(s) 478
BmsI GCATC 2 cut(s) 329, 362
Bsa29I ATCGAT 1 cut(s) 640
BsaBI GATNNNNATC 1 cut(s) 695
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 2 cut(s) 303, 477
BsaWI WCCGGW 1 cut(s) 133
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 2 cut(s) 655, 723
Bse1I ACTGG 1 cut(s) 141
Bse8I GATNNNNATC 1 cut(s) 695
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 1 cut(s) 478
BseCI ATCGAT 1 cut(s) 640
BseDI CCNNGG 2 cut(s) 303, 477
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 2 cut(s) 655, 723
BseNI ACTGG 1 cut(s) 141
BseXI GCAGC 4 cut(s) 60, 63, 341, 634
Bsh1236I CGCG 1 cut(s) 237
BshFI GGCC 4 cut(s) 243, 276, 404, 659
BshNI GGYRCC 1 cut(s) 299
BshVI ATCGAT 1 cut(s) 640
BsiSI CCGG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 655, 723
BsmAI GTCTC 6 cut(s) 130, 131, 149, 408, 511, 526
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 499, 547
BsnI GGCC 4 cut(s) 243, 276, 404, 659
Bso31I GGTCTC 1 cut(s) 131
Bsp13I TCCGGA 1 cut(s) 133
Bsp143I GATC 1 cut(s) 637
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 1 cut(s) 244
BspANI GGCC 4 cut(s) 243, 276, 404, 659
BspDI ATCGAT 1 cut(s) 640
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 1 cut(s) 237
BspLI GGNNCC 5 cut(s) 27, 221, 301, 483, 660
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 131
BsrI ACTGG 1 cut(s) 141
BssECI CCNNGG 2 cut(s) 303, 477
BssMI GATC 1 cut(s) 637
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 303
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 110
BstAPI GCANNNNNTGC 1 cut(s) 326
BstC8I GCNNGC 3 cut(s) 18, 183, 213
BstDEI CTNAG 1 cut(s) 399
BstDSI CCRYGG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 237
BstHHI GCGC 2 cut(s) 237, 361
BstKTI GATC 1 cut(s) 640
BstMAI GTCTC 6 cut(s) 130, 131, 149, 408, 511, 526
BstMBI GATC 1 cut(s) 637
BstMWI GCNNNNNNNGC 3 cut(s) 243, 326, 509
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 476
BstSFI CTRYAG 2 cut(s) 378, 721
BstUI CGCG 1 cut(s) 237
BstV1I GCAGC 4 cut(s) 60, 63, 341, 634
BstXI CCANNNNNNTGG 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 640
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 4 cut(s) 243, 276, 404, 659
BsuTUI ATCGAT 1 cut(s) 640
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 2 cut(s) 252, 682
Cac8I GCNNGC 3 cut(s) 18, 183, 213
CfoI GCGC 2 cut(s) 237, 361
Cfr13I GGNCC 1 cut(s) 658
ClaI ATCGAT 1 cut(s) 640
Csp6I GTAC 1 cut(s) 82
CviAII CATG 3 cut(s) 212, 294, 304
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 1 cut(s) 399
DpnI GATC 1 cut(s) 639
DpnII GATC 1 cut(s) 637
EaeI YGGCCR 2 cut(s) 241, 274
Eco130I CCWWGG 1 cut(s) 303
Eco147I AGGCCT 1 cut(s) 404
Eco31I GGTCTC 1 cut(s) 131
Eco57I CTGAAG 3 cut(s) 354, 477, 638
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 3 cut(s) 215, 297, 307
FaqI GGGAC 1 cut(s) 18
FatI CATG 3 cut(s) 211, 293, 303
FauNDI CATATG 1 cut(s) 580
Fnu4HI GCNGC 5 cut(s) 49, 52, 244, 330, 623
Fsp4HI GCNGC 5 cut(s) 49, 52, 244, 330, 623
FspBI CTAG 1 cut(s) 518
GlaI GCGC 2 cut(s) 236, 360
GluI GCNGC 5 cut(s) 49, 52, 244, 330, 623
HaeIII GGCC 4 cut(s) 243, 276, 404, 659
HapII CCGG 1 cut(s) 134
HhaI GCGC 2 cut(s) 237, 361
Hin1II CATG 3 cut(s) 215, 297, 307
Hin6I GCGC 2 cut(s) 235, 359
HinP1I GCGC 2 cut(s) 235, 359
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 4 cut(s) 6, 344, 562, 575
HpaII CCGG 1 cut(s) 134
HphI GGTGA 1 cut(s) 32
Hpy188I TCNGA 1 cut(s) 737
Hpy188III TCNNGA 4 cut(s) 134, 257, 554, 616
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4V TGCA 8 cut(s) 48, 211, 215, 329, 410, 497, 545, 608
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 326, 509
HpyF3I CTNAG 1 cut(s) 399
Hsp92II CATG 3 cut(s) 215, 297, 307
HspAI GCGC 2 cut(s) 235, 359
Kpn2I TCCGGA 1 cut(s) 133
Kzo9I GATC 1 cut(s) 637
LmnI GCTCC 3 cut(s) 31, 481, 681
Lsp1109I GCAGC 4 cut(s) 60, 63, 341, 634
LweI GCATC 2 cut(s) 329, 362
MaeI CTAG 1 cut(s) 518
MalI GATC 1 cut(s) 639
MboI GATC 1 cut(s) 637
MboII GAAGA 2 cut(s) 347, 723
MfeI CAATTG 1 cut(s) 324
MluCI AATT 4 cut(s) 267, 324, 590, 730
MnlI CCTC 2 cut(s) 69, 102
MroI TCCGGA 1 cut(s) 133
MroXI GAANNNNTTC 1 cut(s) 731
MseI TTAA 3 cut(s) 65, 104, 159
MslI CAYNNNNRTG 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 332
MspI CCGG 1 cut(s) 134
MspR9I CCNGG 1 cut(s) 478
MunI CAATTG 1 cut(s) 324
Mva1269I GAATGC 2 cut(s) 499, 547
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 1 cut(s) 237
MwoI GCNNNNNNNGC 3 cut(s) 243, 326, 509
NcoI CCATGG 1 cut(s) 303
NdeI CATATG 1 cut(s) 580
NdeII GATC 1 cut(s) 637
NlaIII CATG 3 cut(s) 215, 297, 307
NlaIV GGNNCC 5 cut(s) 27, 221, 301, 483, 660
NspI RCATGY 1 cut(s) 215
PaeI GCATGC 1 cut(s) 215
PceI AGGCCT 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 646
PctI GAATGC 2 cut(s) 499, 547
PdmI GAANNNNTTC 1 cut(s) 731
PfeI GAWTC 3 cut(s) 344, 562, 575
PkrI GCNGC 5 cut(s) 50, 53, 245, 331, 624
Psp6I CCWGG 1 cut(s) 476
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 5 cut(s) 27, 221, 301, 483, 660
PspPI GGNCC 1 cut(s) 658
PvuII CAGCTG 1 cut(s) 332
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 3 cut(s) 65, 104, 159
SatI GCNGC 5 cut(s) 49, 52, 244, 330, 623
Sau3AI GATC 1 cut(s) 637
Sau96I GGNCC 1 cut(s) 658
ScrFI CCNGG 1 cut(s) 478
SetI ASST 4 cut(s) 197, 225, 334, 627
SfaNI GCATC 2 cut(s) 329, 362
SfcI CTRYAG 2 cut(s) 378, 721
SmiMI CAYNNNNRTG 1 cut(s) 204
SphI GCATGC 1 cut(s) 215
Sse9I AATT 4 cut(s) 267, 324, 590, 730
SseBI AGGCCT 1 cut(s) 404
SsiI CCGC 1 cut(s) 244
SspMI CTAG 1 cut(s) 518
StuI AGGCCT 1 cut(s) 404
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 110
TaqI TCGA 3 cut(s) 555, 640, 709
TasI AATT 4 cut(s) 267, 324, 590, 730
TauI GCSGC 1 cut(s) 246
TfiI GAWTC 3 cut(s) 344, 562, 575
Tru1I TTAA 3 cut(s) 65, 104, 159
Tru9I TTAA 3 cut(s) 65, 104, 159
TseI GCWGC 4 cut(s) 48, 51, 329, 622
TspDTI ATGAA 2 cut(s) 357, 554
TspGWI ACGGA 1 cut(s) 99
XapI RAATTY 1 cut(s) 590
XceI RCATGY 1 cut(s) 215
XmnI GAANNNNTTC 1 cut(s) 731
XspI CTAG 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.