pycom07g22810
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007003
Physical Location & Seq
Reverse (-)
23992164 .. 23992709
546 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGGAAACTTTTTCACACGTTCCCCCTGGTTTTCGGTTCCATCCGACGGATGAAGAACTTGTTGATTACTACCTCAGGAAGAAGGTTACTTCAAGAAGGATTGACCTTGATGTTATTAAAGATGTCGACCTCTATAAAATTGAGCCATGGGATCTTCAAGAACTATGCAGAATAGGGACAGAAGAACAAAACGAATGGTACTTTTTTAGCCATAAAGATAAGAAGTATCCTACTGGAACTCGAACAAATAGAGCCACCGCTGCAGGGTTTTGGAAAGCCACGGGAAGAGACAAGGCCATTTATTCAAAGCATGACTTAATTGGCATGCGGAAAACCTTAGTGTTTTATAAAGGTCGAGCCCCAAATGGTCAAAAGTCCGATTGGATTATGCATGAATACCGCCTTGAAACTGATGAAAACGGAACTCCACAGGCAAGAATTTAA

Protein Analysis

148

Amino Acids

17.45

Weight (kDa)

8.58

Isoelectric Point (pI)

31.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 8 - 135 9.2e-50 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AccI GTMKAC 1 cut(s) 126
AciI CCGC 3 cut(s) 258, 328, 400
AclWI GGATC 1 cut(s) 159
AcsI RAATTY 1 cut(s) 438
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 46, 264
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 4 cut(s) 93, 158, 306, 407
AjnI CCWGG 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 282
AlwI GGATC 1 cut(s) 159
AoxI GGCC 1 cut(s) 294
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 1 cut(s) 438
AxyI CCTNAGG 1 cut(s) 74
BanII GRGCYC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 247
BccI CCATC 1 cut(s) 48
BciT130I CCWGG 1 cut(s) 27
BciVI GTATCC 1 cut(s) 237
BcoDI GTCTC 1 cut(s) 282
BfmI CTRYAG 1 cut(s) 261
BfuI GTATCC 1 cut(s) 237
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 1 cut(s) 27
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 27
BsaJI CCNNGG 3 cut(s) 25, 146, 279
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 264
Bse1I ACTGG 1 cut(s) 238
Bse21I CCTNAGG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 3 cut(s) 25, 146, 279
BseGI GGATG 2 cut(s) 40, 55
BseLI CCNNNNNNNGG 2 cut(s) 46, 264
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 1 cut(s) 238
BseXI GCAGC 1 cut(s) 247
BshFI GGCC 1 cut(s) 296
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 2 cut(s) 46, 264
BsmAI GTCTC 1 cut(s) 282
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 1 cut(s) 296
Bsp1286I GDGCHC 1 cut(s) 361
Bsp143I GATC 1 cut(s) 151
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 3 cut(s) 258, 328, 400
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 38
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 159
BsrI ACTGG 1 cut(s) 238
BssECI CCNNGG 3 cut(s) 25, 146, 279
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 146
Bst2UI CCWGG 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 280
BstC8I GCNNGC 1 cut(s) 326
BstDEI CTNAG 2 cut(s) 74, 337
BstDSI CCRYGG 2 cut(s) 146, 279
BstF5I GGATG 2 cut(s) 40, 55
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 1 cut(s) 282
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 1 cut(s) 260
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 1 cut(s) 247
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 74
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 1 cut(s) 296
BtgI CCRYGG 2 cut(s) 146, 279
BtsCI GGATG 2 cut(s) 40, 55
Cac8I GCNNGC 1 cut(s) 326
Csp6I GTAC 1 cut(s) 199
CviAII CATG 4 cut(s) 147, 311, 325, 392
CviJI RGCY 6 cut(s) 145, 210, 254, 278, 296, 359
CviKI_1 RGCY 6 cut(s) 145, 210, 254, 278, 296, 359
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 2 cut(s) 74, 337
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 280
EarI CTCTTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 361
Eco81I CCTNAGG 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 4 cut(s) 150, 314, 328, 395
FaiI YATR 9 cut(s) 135, 148, 166, 213, 312, 326, 348, 389, 393
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 1 cut(s) 190
FatI CATG 4 cut(s) 146, 310, 324, 391
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 2 cut(s) 27, 62
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 1 cut(s) 261
GluI GCNGC 1 cut(s) 261
HaeIII GGCC 1 cut(s) 296
Hin1II CATG 4 cut(s) 150, 314, 328, 395
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 45, 379
Hpy188III TCNNGA 3 cut(s) 76, 93, 158
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 2 cut(s) 76, 90
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 3 cut(s) 168, 263, 391
HpyF10VI GCNNNNNNNGC 1 cut(s) 260
HpyF3I CTNAG 2 cut(s) 74, 337
HpySE526I ACGT 1 cut(s) 18
Hsp92II CATG 4 cut(s) 150, 314, 328, 395
Kzo9I GATC 1 cut(s) 151
LpnPI CCDG 6 cut(s) 12, 39, 61, 219, 249, 416
Lsp1109I GCAGC 1 cut(s) 247
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 1 cut(s) 85
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 5 cut(s) 65, 91, 146, 194, 297
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 3 cut(s) 138, 318, 438
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 2 cut(s) 83, 140
Mph1103I ATGCAT 1 cut(s) 393
MseI TTAA 3 cut(s) 117, 317, 442
MspA1I CMGCKG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 260
NcoI CCATGG 1 cut(s) 146
NdeII GATC 1 cut(s) 151
NlaIII CATG 4 cut(s) 150, 314, 328, 395
NlaIV GGNNCC 1 cut(s) 38
NsiI ATGCAT 1 cut(s) 393
NspI RCATGY 1 cut(s) 328
PaeI GCATGC 1 cut(s) 328
PkrI GCNGC 1 cut(s) 262
PsiI TTATAA 1 cut(s) 348
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 38
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 3 cut(s) 117, 317, 442
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 1 cut(s) 151
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 361
SetI ASST 7 cut(s) 21, 75, 87, 108, 132, 338, 355
SfcI CTRYAG 1 cut(s) 261
SphI GCATGC 1 cut(s) 328
Sse9I AATT 3 cut(s) 138, 318, 438
SsiI CCGC 3 cut(s) 258, 328, 400
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 146
TaiI ACGT 1 cut(s) 21
TaqI TCGA 3 cut(s) 126, 241, 355
TasI AATT 3 cut(s) 138, 318, 438
Tru1I TTAA 3 cut(s) 117, 317, 442
Tru9I TTAA 3 cut(s) 117, 317, 442
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 3 cut(s) 66, 408, 429
TspGWI ACGGA 2 cut(s) 62, 435
XapI RAATTY 1 cut(s) 438
XceI RCATGY 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.