pycom07g26010

RWP-RK domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012960
Physical Location & Seq
Forward (+)
26142951 .. 26144889
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 924 bp
ATGGTCCAGAACCGTAACCCCTTTGAGAGCGTCGGCACGGACTCTCTCATCCCATCAATCTCCTTCCCGACCCAGTCAAATTTATACTGCTTAATTCCTCCACCCTCTCTGATAGTTCTCCAAGCTTTCCAAAACTCTCCAATGGCAGATTCCATTTCCTCCCAACTTCTGTTTCCAAAAATTGAGACAGACCCACATGAGTTTAACTCCTCCATACATGCTGCTGCATTAGAAAGTCTGCACCTTGAAAATTTCAATGGTTTCGATTCACCGCCATTGCCATTGGGATGCAAGTCCAACCAGCTTCCAAAAATGGGTTCTCAAGATTTTGAGGATTCAGGCATCTGGGACTACATTTTGAACGACGCTGCTTTTCGATCACCTTTTGGTTACGAGGTGGTTGATGAAAAGCCCCTGACAACAATGGCGGAATCCGTTGACTGTTCGAACAGTGTTTCGGGTGATTCGGACGATGTTTTGAGGACGATCGAGTGGGGGATGAAGATTTGGGGCAAAGATGAAGATGAAAGCAGGACAAAAAATGAGAGTTGTTGCAAGAAGAGGTGTGGCACTCCATTGGAACTGGACGAGATTCAGAAGTATTTCGACGTGCCGATAACTCAGGCGGCCAAGGAGTTGAAGGTGGGATTGACGGTTTTGAAGAGGAGGTGCAGGGAACTGAACATCATGAGATGGCCGCGCAGAAAGATCAAGAGCTTGAATGCTCTCATTGAAAATGTTAAGGGGATGGGATTGACGAATGATGTGATGATGCTGGAGGAGCACAAGAGGGTTCTACAGCAGATGCCGGACATGGAGTTGCCGGAGAGAGCCAAGAGGCTGAGGCAGGCTTGCTTCAAAACCAACTACAAGAAGAAGAGGGCTTTGTGTTTGACTGCTCTGCCGGTCCAAGATTTAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RWP-RK

Protein Analysis

308

Amino Acids

34.77

Weight (kDa)

5.73

Isoelectric Point (pI)

59.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RWP-RK PF02042 193 - 240 2.2e-23 RWP-RK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G53040
fragaria_vesca FvH4_7g31350
malus_domestica MD01G1217600.v1.1 MD07G1286800.v1.1
prunus_persica Prupe.2G308900_v2.0.a1 Prupe.2G308900_v2.0.a1
pyrus_communis pycom01g22770 pycom07g26010
rosa_chinensis RchiOBHm_Chr1g0380421
rosa_laevigata RLG00000026302
rosa_multiflora Rmu_sc0005371.1_g000008
rosa_roxburghii Rroxscaffold_4G00279390
rosa_rugosa Rorug01G0421000
rosa_samantha Rh1AG445300 Rh1BG400600 Rh1CG415000 Rh1DG430700
rosa_wichuraiana Rw1G038750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 700
AciI CCGC 4 cut(s) 272, 428, 626, 698
AcoI YGGCCR 2 cut(s) 627, 695
AcsI RAATTY 2 cut(s) 79, 250
AfiI CCNNNNNNNGG 1 cut(s) 314
AgsI TTSAA 8 cut(s) 248, 256, 361, 640, 661, 721, 734, 859
AjiI CACGTC 1 cut(s) 610
AjuI GAANNNNNNNTTGG 6 cut(s) 133, 156, 165, 188, 301, 333
AluBI AGCT 3 cut(s) 125, 304, 717
AluI AGCT 3 cut(s) 125, 304, 717
Alw21I GWGCWC 1 cut(s) 786
Alw26I GTCTC 1 cut(s) 179
AoxI GGCC 2 cut(s) 627, 695
ApeKI GCWGC 3 cut(s) 221, 224, 368
ApoI RAATTY 2 cut(s) 79, 250
Asp700I GAANNNNTTC 1 cut(s) 602
AspLEI GCGC 1 cut(s) 702
AspS9I GGNCC 2 cut(s) 4, 907
AsuHPI GGTGA 3 cut(s) 261, 372, 473
AsuII TTCGAA 1 cut(s) 446
AvaII GGWCC 2 cut(s) 4, 907
Bbv12I GWGCWC 1 cut(s) 786
BbvCI CCTCAGC 1 cut(s) 842
BbvI GCAGC 3 cut(s) 208, 211, 355
BccI CCATC 3 cut(s) 61, 687, 742
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 922
BfmI CTRYAG 1 cut(s) 797
BisI GCNGC 5 cut(s) 222, 225, 369, 627, 698
BlsI GCNGC 5 cut(s) 223, 226, 370, 628, 699
Bme18I GGWCC 2 cut(s) 4, 907
BmgBI CACGTC 1 cut(s) 610
BmgT120I GGNCC 2 cut(s) 4, 907
BmrI ACTGGG 1 cut(s) 67
BmsI GCATC 4 cut(s) 278, 351, 762, 795
BmuI ACTGGG 1 cut(s) 67
BplI GAGNNNNNCTC 2 cut(s) 191, 223
BpmI CTGGAG 1 cut(s) 797
Bpu10I CCTNAGC 1 cut(s) 842
Bpu14I TTCGAA 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 630
Bsc4I CCNNNNNNNGG 1 cut(s) 314
Bse118I RCCGGY 1 cut(s) 904
Bse1I ACTGG 2 cut(s) 73, 588
Bse3DI GCAATG 1 cut(s) 275
BseDI CCNNGG 1 cut(s) 630
BseGI GGATG 4 cut(s) 48, 293, 504, 753
BseLI CCNNNNNNNGG 1 cut(s) 314
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 2 cut(s) 635, 833
BseNI ACTGG 2 cut(s) 73, 588
BseRI GAGGAG 3 cut(s) 199, 679, 794
BseXI GCAGC 3 cut(s) 208, 211, 355
BsgI GTGCAG 2 cut(s) 224, 691
Bsh1236I CGCG 1 cut(s) 700
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 2 cut(s) 629, 697
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 786
BsiSI CCGG 3 cut(s) 809, 824, 905
BslFI GGGAC 1 cut(s) 362
BslI CCNNNNNNNGG 1 cut(s) 314
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 362
BsmI GAATGC 1 cut(s) 727
BsnI GGCC 2 cut(s) 629, 697
Bsp119I TTCGAA 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 786
Bsp143I GATC 3 cut(s) 377, 486, 708
BspACI CCGC 4 cut(s) 272, 428, 626, 698
BspANI GGCC 2 cut(s) 629, 697
BspCNI CTCAG 2 cut(s) 634, 834
BspFNI CGCG 1 cut(s) 700
BspHI TCATGA 1 cut(s) 687
BspT104I TTCGAA 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 904
BsrI ACTGG 2 cut(s) 73, 588
BssAI RCCGGY 1 cut(s) 904
BssECI CCNNGG 1 cut(s) 630
BssMI GATC 3 cut(s) 377, 486, 708
BssT1I CCWWGG 1 cut(s) 630
Bst4CI ACNGT 4 cut(s) 14, 443, 452, 655
Bst6I CTCTTC 3 cut(s) 554, 656, 872
BstBI TTCGAA 1 cut(s) 446
BstC8I GCNNGC 2 cut(s) 849, 853
BstDEI CTNAG 2 cut(s) 621, 842
BstF5I GGATG 4 cut(s) 48, 293, 504, 753
BstFNI CGCG 1 cut(s) 700
BstHHI GCGC 1 cut(s) 702
BstKTI GATC 3 cut(s) 380, 489, 711
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 3 cut(s) 377, 486, 708
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 1 cut(s) 781
BstNSI RCATGY 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 797
BstUI CGCG 1 cut(s) 700
BstV1I GCAGC 3 cut(s) 208, 211, 355
BsuRI GGCC 2 cut(s) 629, 697
BtrI CACGTC 1 cut(s) 610
BtsCI GGATG 4 cut(s) 48, 293, 504, 753
BtsIMutI CAGTG 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 849, 853
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 702
Cfr10I RCCGGY 1 cut(s) 904
Cfr13I GGNCC 2 cut(s) 4, 907
CseI GACGC 2 cut(s) 19, 374
CviAII CATG 4 cut(s) 197, 218, 688, 814
DdeI CTNAG 2 cut(s) 621, 842
DpnI GATC 3 cut(s) 379, 488, 710
DpnII GATC 3 cut(s) 377, 486, 708
EaeI YGGCCR 2 cut(s) 627, 695
Eam1104I CTCTTC 3 cut(s) 554, 656, 872
EarI CTCTTC 3 cut(s) 554, 656, 872
EciI GGCGGA 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 630
Eco47I GGWCC 2 cut(s) 4, 907
EcoT14I CCWWGG 1 cut(s) 630
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 4 cut(s) 200, 221, 691, 817
FaiI YATR 6 cut(s) 85, 198, 215, 219, 689, 815
FaqI GGGAC 1 cut(s) 362
FatI CATG 4 cut(s) 196, 217, 687, 813
Fnu4HI GCNGC 5 cut(s) 222, 225, 369, 627, 698
FokI GGATG 4 cut(s) 35, 300, 511, 760
Fsp4HI GCNGC 5 cut(s) 222, 225, 369, 627, 698
FspBI CTAG 1 cut(s) 922
GlaI GCGC 1 cut(s) 701
GluI GCNGC 5 cut(s) 222, 225, 369, 627, 698
GsuI CTGGAG 1 cut(s) 797
HaeIII GGCC 2 cut(s) 629, 697
HapII CCGG 3 cut(s) 809, 824, 905
HgaI GACGC 2 cut(s) 19, 374
HhaI GCGC 1 cut(s) 702
Hin1II CATG 4 cut(s) 200, 221, 691, 817
Hin6I GCGC 1 cut(s) 700
HinP1I GCGC 1 cut(s) 700
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 7 cut(s) 41, 149, 266, 335, 431, 464, 592
HpaII CCGG 3 cut(s) 809, 824, 905
HphI GGTGA 3 cut(s) 261, 372, 473
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 3 cut(s) 111, 469, 597
Hpy188III TCNNGA 5 cut(s) 7, 67, 323, 688, 712
Hpy8I GTNNAC 1 cut(s) 439
Hpy99I CGWCG 3 cut(s) 35, 368, 611
HpyAV CCTTC 2 cut(s) 73, 634
HpyCH4III ACNGT 4 cut(s) 14, 443, 452, 655
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 5 cut(s) 227, 241, 291, 555, 672
HpyF10VI GCNNNNNNNGC 1 cut(s) 781
HpyF3I CTNAG 2 cut(s) 621, 842
HpySE526I ACGT 1 cut(s) 609
Hsp92II CATG 4 cut(s) 200, 221, 691, 817
HspAI GCGC 1 cut(s) 700
Kzo9I GATC 3 cut(s) 377, 486, 708
LmnI GCTCC 1 cut(s) 781
Lsp1109I GCAGC 3 cut(s) 208, 211, 355
LweI GCATC 4 cut(s) 278, 351, 762, 795
MaeI CTAG 1 cut(s) 922
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 2 cut(s) 14, 389
MalI GATC 3 cut(s) 379, 488, 710
MboI GATC 3 cut(s) 377, 486, 708
MboII GAAGA 6 cut(s) 514, 533, 571, 673, 886, 889
MhlI GDGCHC 1 cut(s) 786
MluCI AATT 4 cut(s) 79, 93, 180, 250
MlyI GAGTC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 602
MseI TTAA 3 cut(s) 92, 204, 741
MslI CAYNNNNRTG 1 cut(s) 286
MspI CCGG 3 cut(s) 809, 824, 905
Mva1269I GAATGC 1 cut(s) 727
MvnI CGCG 1 cut(s) 700
MwoI GCNNNNNNNGC 1 cut(s) 781
NdeII GATC 3 cut(s) 377, 486, 708
NlaIII CATG 4 cut(s) 200, 221, 691, 817
NspI RCATGY 1 cut(s) 221
NspV TTCGAA 1 cut(s) 446
PagI TCATGA 1 cut(s) 687
PctI GAATGC 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 602
PfeI GAWTC 6 cut(s) 149, 266, 335, 431, 464, 592
PflFI GACNNNGTC 1 cut(s) 73
PkrI GCNGC 5 cut(s) 223, 226, 370, 628, 699
Ple19I CGATCG 1 cut(s) 489
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PspPI GGNCC 2 cut(s) 4, 907
PsyI GACNNNGTC 1 cut(s) 73
PvuI CGATCG 1 cut(s) 489
RseI CAYNNNNRTG 1 cut(s) 286
SaqAI TTAA 3 cut(s) 92, 204, 741
SatI GCNGC 5 cut(s) 222, 225, 369, 627, 698
Sau3AI GATC 3 cut(s) 377, 486, 708
Sau96I GGNCC 2 cut(s) 4, 907
SchI GAGTC 1 cut(s) 35
SduI GDGCHC 1 cut(s) 786
SfaNI GCATC 4 cut(s) 278, 351, 762, 795
SfcI CTRYAG 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 446
SinI GGWCC 2 cut(s) 4, 907
SmiMI CAYNNNNRTG 1 cut(s) 286
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 4 cut(s) 79, 93, 180, 250
SsiI CCGC 4 cut(s) 272, 428, 626, 698
SspMI CTAG 1 cut(s) 922
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 4 cut(s) 14, 443, 452, 655
TaiI ACGT 1 cut(s) 612
TaqI TCGA 5 cut(s) 264, 376, 446, 489, 606
TasI AATT 4 cut(s) 79, 93, 180, 250
TauI GCSGC 2 cut(s) 629, 700
TfiI GAWTC 6 cut(s) 149, 266, 335, 431, 464, 592
Tru1I TTAA 3 cut(s) 92, 204, 741
Tru9I TTAA 3 cut(s) 92, 204, 741
TscAI CASTG 1 cut(s) 457
TseI GCWGC 3 cut(s) 221, 224, 368
TspDTI ATGAA 4 cut(s) 420, 515, 534, 540
TspGWI ACGGA 2 cut(s) 53, 424
TspRI CASTG 1 cut(s) 457
Tth111I GACNNNGTC 1 cut(s) 73
VpaK11BI GGWCC 2 cut(s) 4, 907
XapI RAATTY 2 cut(s) 79, 250
XceI RCATGY 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 602
XspI CTAG 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.