pycom07g27590

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007447
Physical Location & Seq
Reverse (-)
27254789 .. 27256305
1517 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 810 bp
ATGCAGCTAGAAGTTCTGGATTACATTTTGGTACCGGCAGGCCTCCTACTGATGGTGACTTACCATCTATGGCTTCTGTGCCGAATCGTTAAGCAACCCAACGAGACCGTCATCGGCATCAACTCCATCAATCGCCGCTTCTGGGTCCAAGCCATGATGGAGGATGCTCCAAAGAATGGTGTTCTTCCGGTGCAGACGCTTCGAAACAACATAATGGCATCGACCCTTTTGGCCTCGACCGCCATTACGCTCTGCTCTCTCATTGCCCTCTTGATGACCAGCGGCACCAAATACAGAACGACTTTATTCGTCTTTGGGGACCGAACCGAACTTGCATTTTCAATCAAGTTCTTCGCCATTTTGGTGTGTTTCTTGGTGGCATTCCTCTTCAACGTGCAGTCCATAAGGTACTACAGCCATGCCAGCATGCTCATCAACACGCCGTTCAAGAGGATGATGTCGCCGCCGCACCACAAGCAGCAGTACCTCACGACAGAGTACGTTGCCACCACCGTGAACCGAGGCAGCTATTTCTGGTCCTTGGGCCTCCGAGCATTCTACTTCTCGTTCCCTCTGTTTCTGTGGATTTTCGGGCCGATTCCTATGTTCTTGTCGTGCATTGTTTTGGTGATCTTGCTGTATTTTTTGGATGTCACCTTTCAGTTCAATTGGGTTGTTGCGGTTGCTGGGGATGAAGACCCGGTCCTCATTAAGGGTGATGAGGAGCAAGGCTTGTTCAATGCACCAGGGATCACACATTGTGACGGTACCTCAAGCGAAGTGTGTGGGAAACAGTGTGACAGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

270

Amino Acids

30.49

Weight (kDa)

6.94

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 220 1.6e-75 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 31, 767
AccB1I GGYRCC 3 cut(s) 31, 284, 767
AccB7I CCANNNNNTGG 1 cut(s) 176
AciI CCGC 6 cut(s) 136, 240, 282, 464, 467, 680
AclWI GGATC 1 cut(s) 758
AdeI CACNNNGTG 1 cut(s) 761
AfaI GTAC 5 cut(s) 33, 410, 485, 500, 769
AfiI CCNNNNNNNGG 4 cut(s) 52, 142, 176, 712
AgsI TTSAA 5 cut(s) 342, 391, 448, 667, 739
AjnI CCWGG 1 cut(s) 745
AleI CACNNNNGTG 1 cut(s) 512
AluBI AGCT 2 cut(s) 7, 528
AluI AGCT 2 cut(s) 7, 528
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 1 cut(s) 758
AoxI GGCC 4 cut(s) 40, 231, 544, 593
ApeKI GCWGC 3 cut(s) 4, 478, 525
Asp718I GGTACC 2 cut(s) 31, 767
AspS9I GGNCC 6 cut(s) 145, 319, 537, 544, 593, 703
AsuC2I CCSGG 1 cut(s) 701
AsuHPI GGTGA 4 cut(s) 67, 640, 646, 728
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 4 cut(s) 145, 319, 537, 703
BanI GGYRCC 3 cut(s) 31, 284, 767
BbsI GAAGAC 1 cut(s) 702
BbvI GCAGC 3 cut(s) 16, 490, 537
BccI CCATC 4 cut(s) 46, 72, 134, 151
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 1 cut(s) 747
BcnI CCSGG 1 cut(s) 701
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 1 cut(s) 8
BfmI CTRYAG 1 cut(s) 412
BisI GCNGC 7 cut(s) 5, 136, 283, 464, 467, 479, 526
BlsI GCNGC 7 cut(s) 6, 137, 284, 465, 468, 480, 527
Bme1390I CCNGG 2 cut(s) 701, 747
Bme18I GGWCC 4 cut(s) 145, 319, 537, 703
BmgT120I GGNCC 6 cut(s) 145, 319, 537, 544, 593, 703
BmiI GGNNCC 5 cut(s) 33, 146, 286, 320, 769
BmrFI CCNGG 2 cut(s) 701, 747
BmsI GCATC 3 cut(s) 126, 154, 227
BpiI GAAGAC 1 cut(s) 702
Bpu14I TTCGAA 1 cut(s) 202
BpuEI CTTGAG 1 cut(s) 757
BpuMI CCSGG 1 cut(s) 701
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 3 cut(s) 520, 540, 746
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 142, 176, 712
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 747
BseDI CCNNGG 3 cut(s) 520, 540, 746
BseGI GGATG 4 cut(s) 169, 459, 655, 697
BseLI CCNNNNNNNGG 4 cut(s) 52, 142, 176, 712
BseMI GCAATG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 3 cut(s) 16, 490, 537
BseYI CCCAGC 1 cut(s) 686
BsgI GTGCAG 2 cut(s) 212, 416
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 4 cut(s) 42, 233, 546, 595
BshNI GGYRCC 3 cut(s) 31, 284, 767
BsiEI CGRYCG 1 cut(s) 240
BsiSI CCGG 3 cut(s) 35, 188, 701
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 4 cut(s) 52, 142, 176, 712
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 332
BsmI GAATGC 2 cut(s) 380, 554
BsnI GGCC 4 cut(s) 42, 233, 546, 595
Bso31I GGTCTC 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 202
Bsp143I GATC 2 cut(s) 630, 750
BspACI CCGC 6 cut(s) 136, 240, 282, 464, 467, 680
BspANI GGCC 4 cut(s) 42, 233, 546, 595
BspLI GGNNCC 5 cut(s) 33, 146, 286, 320, 769
BspPI GGATC 1 cut(s) 758
BspT104I TTCGAA 1 cut(s) 202
BspT107I GGYRCC 3 cut(s) 31, 284, 767
BspTNI GGTCTC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 520, 540, 746
BssMI GATC 2 cut(s) 630, 750
BssT1I CCWWGG 1 cut(s) 540
Bst2UI CCWGG 1 cut(s) 747
Bst4CI ACNGT 5 cut(s) 109, 514, 767, 795, 803
Bst6I CTCTTC 1 cut(s) 392
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 3 cut(s) 40, 424, 428
BstENI CCTNNNNNAGG 1 cut(s) 710
BstF5I GGATG 4 cut(s) 169, 459, 655, 697
BstKTI GATC 2 cut(s) 633, 753
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 2 cut(s) 630, 750
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 3 cut(s) 239, 423, 475
BstNI CCWGG 1 cut(s) 747
BstNSI RCATGY 1 cut(s) 430
BstSCI CCNGG 2 cut(s) 699, 745
BstSFI CTRYAG 1 cut(s) 412
BstV1I GCAGC 3 cut(s) 16, 490, 537
BstV2I GAAGAC 1 cut(s) 702
BsuRI GGCC 4 cut(s) 42, 233, 546, 595
BtsCI GGATG 4 cut(s) 169, 459, 655, 697
BtsIMutI CAGTG 2 cut(s) 800, 808
Cac8I GCNNGC 3 cut(s) 40, 424, 428
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 6 cut(s) 145, 319, 537, 544, 593, 703
CseI GACGC 1 cut(s) 205
Csp6I GTAC 5 cut(s) 32, 409, 484, 499, 768
CviAII CATG 3 cut(s) 154, 419, 427
CviQI GTAC 5 cut(s) 32, 409, 484, 499, 768
DpnI GATC 2 cut(s) 632, 752
DpnII GATC 2 cut(s) 630, 750
DraIII CACNNNGTG 1 cut(s) 761
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 540
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 4 cut(s) 145, 319, 537, 703
EcoNI CCTNNNNNAGG 1 cut(s) 710
EcoRII CCWGG 1 cut(s) 745
EcoT14I CCWWGG 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 540
FaeI CATG 3 cut(s) 157, 422, 430
FaiI YATR 7 cut(s) 70, 155, 212, 404, 420, 428, 605
FaqI GGGAC 1 cut(s) 332
FatI CATG 3 cut(s) 153, 418, 426
Fnu4HI GCNGC 7 cut(s) 5, 136, 283, 464, 467, 479, 526
FokI GGATG 4 cut(s) 176, 466, 662, 704
Fsp4HI GCNGC 7 cut(s) 5, 136, 283, 464, 467, 479, 526
FspBI CTAG 1 cut(s) 8
GluI GCNGC 7 cut(s) 5, 136, 283, 464, 467, 479, 526
GsaI CCCAGC 1 cut(s) 690
HaeIII GGCC 4 cut(s) 42, 233, 546, 595
HapII CCGG 3 cut(s) 35, 188, 701
HgaI GACGC 1 cut(s) 205
Hin1II CATG 3 cut(s) 157, 422, 430
HinfI GANTC 2 cut(s) 84, 598
HpaII CCGG 3 cut(s) 35, 188, 701
HphI GGTGA 4 cut(s) 67, 640, 646, 728
Hpy166II GTNNAC 1 cut(s) 517
Hpy188I TCNGA 1 cut(s) 551
Hpy188III TCNNGA 4 cut(s) 17, 271, 448, 490
Hpy8I GTNNAC 1 cut(s) 517
HpyCH4III ACNGT 5 cut(s) 109, 514, 767, 795, 803
HpyCH4IV ACGT 2 cut(s) 393, 501
HpyCH4V TGCA 6 cut(s) 4, 193, 335, 397, 618, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 423, 475
HpySE526I ACGT 2 cut(s) 393, 501
Hsp92II CATG 3 cut(s) 157, 422, 430
KpnI GGTACC 2 cut(s) 35, 771
Kzo9I GATC 2 cut(s) 630, 750
LmnI GCTCC 2 cut(s) 172, 724
Lsp1109I GCAGC 3 cut(s) 16, 490, 537
LweI GCATC 3 cut(s) 126, 154, 227
MaeI CTAG 1 cut(s) 8
MaeII ACGT 2 cut(s) 393, 501
MaeIII GTNAC 4 cut(s) 55, 652, 761, 797
MalI GATC 2 cut(s) 632, 752
MboI GATC 2 cut(s) 630, 750
MboII GAAGA 4 cut(s) 176, 343, 379, 707
MfeI CAATTG 1 cut(s) 667
MluCI AATT 1 cut(s) 667
MseI TTAA 2 cut(s) 90, 711
MslI CAYNNNNRTG 2 cut(s) 362, 512
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 3 cut(s) 35, 188, 701
MspR9I CCNGG 2 cut(s) 701, 747
MunI CAATTG 1 cut(s) 667
Mva1269I GAATGC 2 cut(s) 380, 554
MvaI CCWGG 1 cut(s) 747
MwoI GCNNNNNNNGC 3 cut(s) 239, 423, 475
NciI CCSGG 1 cut(s) 701
NdeII GATC 2 cut(s) 630, 750
NlaIII CATG 3 cut(s) 157, 422, 430
NlaIV GGNNCC 5 cut(s) 33, 146, 286, 320, 769
NmuCI GTSAC 4 cut(s) 55, 652, 761, 797
NspI RCATGY 1 cut(s) 430
NspV TTCGAA 1 cut(s) 202
OliI CACNNNNGTG 1 cut(s) 512
PaeI GCATGC 1 cut(s) 430
PceI AGGCCT 1 cut(s) 42
PctI GAATGC 2 cut(s) 380, 554
PfeI GAWTC 2 cut(s) 84, 598
PflFI GACNNNGTC 1 cut(s) 701
PflMI CCANNNNNTGG 1 cut(s) 176
PkrI GCNGC 7 cut(s) 6, 137, 284, 465, 468, 480, 527
Psp6I CCWGG 1 cut(s) 745
PspFI CCCAGC 1 cut(s) 686
PspGI CCWGG 1 cut(s) 745
PspN4I GGNNCC 5 cut(s) 33, 146, 286, 320, 769
PspPI GGNCC 6 cut(s) 145, 319, 537, 544, 593, 703
PsyI GACNNNGTC 1 cut(s) 701
RsaI GTAC 5 cut(s) 33, 410, 485, 500, 769
RsaNI GTAC 5 cut(s) 32, 409, 484, 499, 768
RseI CAYNNNNRTG 2 cut(s) 362, 512
SaqAI TTAA 2 cut(s) 90, 711
SatI GCNGC 7 cut(s) 5, 136, 283, 464, 467, 479, 526
Sau3AI GATC 2 cut(s) 630, 750
Sau96I GGNCC 6 cut(s) 145, 319, 537, 544, 593, 703
ScrFI CCNGG 2 cut(s) 701, 747
SetI ASST 8 cut(s) 9, 396, 410, 489, 504, 530, 659, 773
SfaNI GCATC 3 cut(s) 126, 154, 227
SfcI CTRYAG 1 cut(s) 412
SfuI TTCGAA 1 cut(s) 202
SinI GGWCC 4 cut(s) 145, 319, 537, 703
SmiMI CAYNNNNRTG 2 cut(s) 362, 512
SmlI CTYRAG 1 cut(s) 772
SmoI CTYRAG 1 cut(s) 772
SphI GCATGC 1 cut(s) 430
Sse9I AATT 1 cut(s) 667
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 6 cut(s) 136, 240, 282, 464, 467, 680
SspMI CTAG 1 cut(s) 8
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 699, 745
StyI CCWWGG 1 cut(s) 540
TaaI ACNGT 5 cut(s) 109, 514, 767, 795, 803
TaiI ACGT 2 cut(s) 396, 504
TaqI TCGA 3 cut(s) 202, 221, 236
TaqII GACCGA 1 cut(s) 336
TasI AATT 1 cut(s) 667
TauI GCSGC 4 cut(s) 138, 285, 466, 469
TfiI GAWTC 2 cut(s) 84, 598
Tru1I TTAA 2 cut(s) 90, 711
Tru9I TTAA 2 cut(s) 90, 711
TscAI CASTG 2 cut(s) 800, 808
TseFI GTSAC 4 cut(s) 55, 652, 761, 797
TseI GCWGC 3 cut(s) 4, 478, 525
Tsp45I GTSAC 4 cut(s) 55, 652, 761, 797
TspDTI ATGAA 1 cut(s) 708
TspRI CASTG 2 cut(s) 800, 808
Tth111I GACNNNGTC 1 cut(s) 701
Van91I CCANNNNNTGG 1 cut(s) 176
VpaK11BI GGWCC 4 cut(s) 145, 319, 537, 703
XagI CCTNNNNNAGG 1 cut(s) 710
XceI RCATGY 1 cut(s) 430
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.