pycom07g27910

PWWP domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006536
Physical Location & Seq
Reverse (-)
27432788 .. 27436704
3917 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2322 bp
ATGTGTGTTATGGGTAGTGCGGATTCAGGTGCGGGCGATTTCAGCGTAGGATCGATCGTCTGGGTGAGGCGGAGGAACGGGTCCTGGTGGCCGGGGAAGATAGTGGGCCCGGAAGAGCTCTCCACTTCGCATCTCACGTCGCCTCGCTCTGGGACCCCCGTCAAGCTTCTCGGCAGAGAAGATGCCAGTGTTGACTGGTACAATCTGGAAAAATCCAAGCGTGTTAAGGCATTTCGATGCGGTGAGTTTGATGATTGTATTGAGAAGGCCGAATCAGCGCAGGGTATGCCTATAAAGAAAAGAGAGAAATATGCACGTCGAGAAGATGCTATTCTTCATGCGCTTGAACTTGAGAAGCAGTTATTGAAGAAACAAGGAAAGTTAGGTATAACTTCTGAACGCTTGAACAATAAATTATCTGATGCTGTGAAAAAGGAGTTAGTTATTTCTTCTGGGAGCTTGGGAATTGACAATGTAAAACTTGCAAATTCCAAATCACCTACGTCTCATAGAAATGACATTATAGGCAGTCCTATGTCTTCAGTAAGAGTTGAAGAGGGAAATCAACTAAGTGGTGATGATGATCATTCTGAAGTCGTGCCTCGGATGAGAGGCTTGCAAGACTTTGGTCTCAAAATTGCCCCTTCGAAGCGAAAAATTTCTTCTATTGCTTTAAACGGTTCTTTGAAACCCACACTTAATGGTAATGTTCAAGCTCTTAGTCGCGGTGGTCTTAGCATGGGAGGAACAAGTCATGTGAATGGTAAACATTCTTTAGAGCAAAGGAAAAGGTCACATGATGGATTAAGTGATGAAATTTTGGTCAAGAGACCTGATAAACGTCGTCCTCTTGTTCAAGTTTTGCAGAATATTGCAAAATTAGCAGTCCCACAGTCCTTGCAACCTGACAGTACTACTCTTTCTACTTCTGTTTCAGGAGTGGAGCCAACGGGAGTTATTTACAAGGCAAAGAGGAGTAAAATTGTATATTTGCCAGCTGAGTCTGGTGAATCCTTGGGGTATGAAGCATCTCCTTCAAACCATGTAGAGATCTCAGCTTCCCCAGTTGGTGCTCGTCCTCATGCTGAGTCCATGATTTCAGAGAACACTTCTGGTTTTACAGAGGATGACTCTGATTCTTCTGAAACTGATACTTCTGAGTCCGAGTTGGATTCATCTGAGACCGAACCAGATATGGATGAAGACATGCCTTTACTTTCAGAACCAGAAGTGGGAAGATATGAAGCACAAGAACACAGAATCATGGTTGGTGAGGAGCCTGGTGAATTATCGCATTCTGGTGAGATGTCTCACCTCTATAGTCATGACTCTCTATTTGCTAGTGGAGCTGTGTCCAAATGGCAGCTGAAAGGGAAAAGGAATATCCGCAATCTTACAAAAAAGTCTATGGATGCAACTTACGGAAAAGGCTATAATTATGGACCGTATTCTGAAGAAAAGACTGAGTTTGTACTGGAAGATGAATACTCCATGGCGTCAAGAGCTTCAGCAAGACATCAAAACAGTGTTGGTCGGAATATGATTGATTGGGAGGACTGGACCTGGGAGGATAGGTCTGCTTGGAACGAATACTGGGACATCAAAAGAGAGCGCTTTCACCCGGTATATGATGGCCGTCATCATTATCGCAGGAGGCCAAAATACTTGATAGATGTTGACTTGAAGGTCCAGGCAAGTTACCAAAAGGAGCCTGTTCCAATTGTTTCTCTGATGAGTAAGTTAAATGGGAAAGCAATAATAGGGCACCCAATCCAAATTGAAGCCTTAGAAGATGGTTCATCCAACTCGCTTCTTTCAACAGTTGATGAATTTGGTGAAGAAGCAATTGACAATAATGGGGTTGCAACTCTTCCACACGCATGGAGGACGGCAAGGAGGACAGCAAATGTTCGAGTCCCACGCCCTCATTTATCATCTGCATTGGATGGTGATGAAACTGCTAACTACCCTCCCTTTTCAGATGAAGAAAGCAGACTGCCATTTAAGAAACTAAATATAGGGAGTTTCAGTAACAAGGCAAGCCAGGGCAAAAGGAGCCTTCCTTACAACTCTCGGCTTCCCACTGATAGAAAGTTCTCAAAAAAGGCGGCCAAGAAAGTGATCTTATCGTCAAGCCAGAAAACGAGGACCATATCTTCAATACCCGTTGAACGGAATTTTAGTAACAAGCGAATGCATCATGATAGCAGTAGTTATCAAAGGGAAGGGCTGATCAAACCGGAGTCTTCTGGGGCTGCTACTACTGTAGCTTGCATACCGGTGAAATTAGTATTCAGTAGGTTACTTGAGAACGATCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PWWP

Protein Analysis

774

Amino Acids

85.77

Weight (kDa)

7.72

Isoelectric Point (pI)

53.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PWWP PF00855 17 - 97 2.5e-10 PWWP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1685
AccB1I GGYRCC 1 cut(s) 1764
AccII CGCG 1 cut(s) 726
AciI CCGC 7 cut(s) 20, 32, 70, 240, 726, 1387, 2108
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 3 cut(s) 89, 1633, 2109
AcsI RAATTY 5 cut(s) 487, 657, 816, 1829, 2176
AcuI CTGAAG 4 cut(s) 525, 612, 1473, 1491
AcyI GRCGYC 1 cut(s) 1496
AfaI GTAC 3 cut(s) 200, 913, 1473
AfeI AGCGCT 1 cut(s) 1613
AfiI CCNNNNNNNGG 3 cut(s) 149, 1232, 2172
AgeI ACCGGT 1 cut(s) 2278
AjiI CACGTC 2 cut(s) 138, 317
AjnI CCWGG 5 cut(s) 83, 1279, 1562, 1689, 2043
Alw21I GWGCWC 2 cut(s) 120, 1075
Alw26I GTCTC 5 cut(s) 510, 635, 823, 1175, 1314
AlwI GGATC 1 cut(s) 58
Aor51HI AGCGCT 1 cut(s) 1613
AoxI GGCC 6 cut(s) 89, 106, 267, 1633, 1655, 2109
ApaI GGGCCC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 1363, 2255
ApoI RAATTY 5 cut(s) 487, 657, 816, 1829, 2176
ArsI GACNNNNNNTTYG 2 cut(s) 1319, 1351
AsiGI ACCGGT 1 cut(s) 2278
Asp700I GAANNNNTTC 1 cut(s) 658
AspLEI GCGC 3 cut(s) 280, 343, 1614
AspS9I GGNCC 8 cut(s) 81, 106, 107, 153, 1442, 1560, 1687, 2148
AsuC2I CCSGG 3 cut(s) 93, 110, 1622
AsuII TTCGAA 1 cut(s) 647
AvaII GGWCC 6 cut(s) 81, 153, 1442, 1560, 1687, 2148
BaeGI GKGCMC 2 cut(s) 110, 1767
BanI GGYRCC 1 cut(s) 1764
BanII GRGCYC 2 cut(s) 110, 120
BbsI GAAGAC 3 cut(s) 531, 1209, 2238
Bbv12I GWGCWC 2 cut(s) 120, 1075
BbvI GCAGC 2 cut(s) 1375, 2242
BccI CCATC 4 cut(s) 794, 1625, 1787, 1940
BceAI ACGGC 2 cut(s) 1620, 1905
BciT130I CCWGG 5 cut(s) 85, 1281, 1564, 1691, 2045
BclI TGATCA 2 cut(s) 583, 2232
BcnI CCSGG 3 cut(s) 93, 110, 1622
BcoDI GTCTC 5 cut(s) 510, 635, 823, 1175, 1314
BfaI CTAG 1 cut(s) 1341
BfmI CTRYAG 2 cut(s) 1318, 2265
BfoI RGCGCY 1 cut(s) 1615
BglII AGATCT 1 cut(s) 1050
BisI GCNGC 3 cut(s) 1364, 2109, 2256
BlsI GCNGC 3 cut(s) 1365, 2110, 2257
BmcAI AGTACT 1 cut(s) 913
Bme1390I CCNGG 8 cut(s) 85, 93, 110, 1281, 1564, 1622, 1691, 2045
Bme18I GGWCC 6 cut(s) 81, 153, 1442, 1560, 1687, 2148
BmgBI CACGTC 2 cut(s) 138, 317
BmgT120I GGNCC 8 cut(s) 81, 106, 107, 153, 1442, 1560, 1687, 2148
BmiI GGNNCC 9 cut(s) 82, 108, 154, 155, 945, 1278, 1710, 1766, 2057
BmrFI CCNGG 8 cut(s) 85, 93, 110, 1281, 1564, 1622, 1691, 2045
BmrI ACTGGG 2 cut(s) 1058, 1603
BmsI GCATC 8 cut(s) 139, 172, 227, 316, 412, 1037, 1402, 2206
BmuI ACTGGG 2 cut(s) 1058, 1603
BoxI GACNNNNGTC 2 cut(s) 158, 627
BpiI GAAGAC 3 cut(s) 531, 1209, 2238
BplI GAGNNNNNCTC 2 cut(s) 1115, 1147
Bpu14I TTCGAA 1 cut(s) 647
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 3 cut(s) 93, 110, 1622
Bsa29I ATCGAT 1 cut(s) 53
BsaBI GATNNNNATC 1 cut(s) 582
BsaHI GRCGYC 1 cut(s) 1496
BsaI GGTCTC 3 cut(s) 635, 823, 1175
BsaJI CCNNGG 6 cut(s) 92, 602, 1014, 1491, 1563, 2044
BsaWI WCCGGW 2 cut(s) 2239, 2278
BsaXI ACNNNNNCTCC 4 cut(s) 1559, 1589, 2014, 2044
Bsc4I CCNNNNNNNGG 3 cut(s) 149, 1232, 2172
Bse118I RCCGGY 1 cut(s) 2278
Bse1I ACTGG 6 cut(s) 186, 200, 1064, 1479, 1562, 1598
Bse8I GATNNNNATC 1 cut(s) 582
BseBI CCWGG 5 cut(s) 85, 1281, 1564, 1691, 2045
BseCI ATCGAT 1 cut(s) 53
BseDI CCNNGG 6 cut(s) 92, 602, 1014, 1491, 1563, 2044
BseGI GGATG 6 cut(s) 612, 1132, 1204, 1417, 1799, 1951
BseJI GATNNNNATC 1 cut(s) 582
BseLI CCNNNNNNNGG 3 cut(s) 149, 1232, 2172
BseMII CTCAG 6 cut(s) 990, 1068, 1077, 1149, 1170, 1455
BseNI ACTGG 6 cut(s) 186, 200, 1064, 1479, 1562, 1598
BseRI GAGGAG 2 cut(s) 988, 1289
BseSI GKGCMC 2 cut(s) 110, 1767
BseXI GCAGC 2 cut(s) 1375, 2242
Bsh1236I CGCG 1 cut(s) 726
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 6 cut(s) 91, 108, 269, 1635, 1657, 2111
BshNI GGYRCC 1 cut(s) 1764
BshTI ACCGGT 1 cut(s) 2278
BshVI ATCGAT 1 cut(s) 53
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 120, 1075
BsiSI CCGG 5 cut(s) 92, 110, 1622, 2240, 2279
BslFI GGGAC 4 cut(s) 166, 872, 1610, 1901
BslI CCNNNNNNNGG 3 cut(s) 149, 1232, 2172
BsmAI GTCTC 5 cut(s) 510, 635, 823, 1175, 1314
BsmBI CGTCTC 1 cut(s) 510
BsmFI GGGAC 4 cut(s) 166, 872, 1610, 1901
BsmI GAATGC 2 cut(s) 1294, 2199
BsnI GGCC 6 cut(s) 91, 108, 269, 1635, 1657, 2111
Bso31I GGTCTC 3 cut(s) 635, 823, 1175
Bsp119I TTCGAA 1 cut(s) 647
Bsp120I GGGCCC 1 cut(s) 106
Bsp1286I GDGCHC 4 cut(s) 110, 120, 1075, 1767
Bsp143I GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
Bsp19I CCATGG 1 cut(s) 1491
BspACI CCGC 7 cut(s) 20, 32, 70, 240, 726, 1387, 2108
BspANI GGCC 6 cut(s) 91, 108, 269, 1635, 1657, 2111
BspCNI CTCAG 6 cut(s) 991, 1067, 1078, 1150, 1171, 1456
BspDI ATCGAT 1 cut(s) 53
BspFNI CGCG 1 cut(s) 726
BspHI TCATGA 2 cut(s) 1324, 2200
BspLI GGNNCC 9 cut(s) 82, 108, 154, 155, 945, 1278, 1710, 1766, 2057
BspPI GGATC 1 cut(s) 58
BspQI GCTCTTC 1 cut(s) 108
BspT104I TTCGAA 1 cut(s) 647
BspT107I GGYRCC 1 cut(s) 1764
BspTNI GGTCTC 3 cut(s) 635, 823, 1175
BsrFI RCCGGY 1 cut(s) 2278
BsrI ACTGG 6 cut(s) 186, 200, 1064, 1479, 1562, 1598
BssAI RCCGGY 1 cut(s) 2278
BssECI CCNNGG 6 cut(s) 92, 602, 1014, 1491, 1563, 2044
BssMI GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
BssNI GRCGYC 1 cut(s) 1496
BssT1I CCWWGG 2 cut(s) 1014, 1491
Bst2UI CCWGG 5 cut(s) 85, 1281, 1564, 1691, 2045
Bst4CI ACNGT 7 cut(s) 680, 894, 911, 1446, 1526, 1822, 2266
Bst6I CTCTTC 3 cut(s) 108, 549, 1875
BstACI GRCGYC 1 cut(s) 1496
BstAPI GCANNNNNTGC 1 cut(s) 286
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 5 cut(s) 34, 617, 996, 2041, 2272
BstDSI CCRYGG 1 cut(s) 1491
BstF5I GGATG 6 cut(s) 612, 1132, 1204, 1417, 1799, 1951
BstFNI CGCG 1 cut(s) 726
BstH2I RGCGCY 1 cut(s) 1615
BstHHI GCGC 3 cut(s) 280, 343, 1614
BstKTI GATC 7 cut(s) 53, 57, 586, 1053, 2124, 2235, 2317
BstMAI GTCTC 5 cut(s) 510, 635, 823, 1175, 1314
BstMBI GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 7 cut(s) 42, 275, 286, 881, 1346, 1502, 2055
BstNI CCWGG 5 cut(s) 85, 1281, 1564, 1691, 2045
BstNSI RCATGY 1 cut(s) 1210
BstPAI GACNNNNGTC 2 cut(s) 158, 627
BstSCI CCNGG 8 cut(s) 83, 91, 108, 1279, 1562, 1620, 1689, 2043
BstSFI CTRYAG 2 cut(s) 1318, 2265
BstSLI GKGCMC 2 cut(s) 110, 1767
BstUI CGCG 1 cut(s) 726
BstV1I GCAGC 2 cut(s) 1375, 2242
BstV2I GAAGAC 3 cut(s) 531, 1209, 2238
BstX2I RGATCY 1 cut(s) 1050
BstXI CCANNNNNNTGG 1 cut(s) 1881
BstYI RGATCY 1 cut(s) 1050
Bsu15I ATCGAT 1 cut(s) 53
BsuRI GGCC 6 cut(s) 91, 108, 269, 1635, 1657, 2111
BsuTUI ATCGAT 1 cut(s) 53
BtgI CCRYGG 1 cut(s) 1491
BtrI CACGTC 2 cut(s) 138, 317
BtsCI GGATG 6 cut(s) 612, 1132, 1204, 1417, 1799, 1951
BtsIMutI CAGTG 3 cut(s) 193, 1531, 2082
Cac8I GCNNGC 5 cut(s) 34, 617, 996, 2041, 2272
CciI TCATGA 2 cut(s) 1324, 2200
CfoI GCGC 3 cut(s) 280, 343, 1614
Cfr10I RCCGGY 1 cut(s) 2278
Cfr13I GGNCC 8 cut(s) 81, 106, 107, 153, 1442, 1560, 1687, 2148
ClaI ATCGAT 1 cut(s) 53
CseI GACGC 1 cut(s) 1485
Csp6I GTAC 3 cut(s) 199, 912, 1472
CspAI ACCGGT 1 cut(s) 2278
CspCI CAANNNNNGTGG 2 cut(s) 879, 914
CviQI GTAC 3 cut(s) 199, 912, 1472
DpnI GATC 7 cut(s) 52, 56, 585, 1052, 2123, 2234, 2316
DpnII GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
DraI TTTAAA 1 cut(s) 675
DrdI GACNNNNNNGTC 1 cut(s) 1685
DseDI GACNNNNNNGTC 1 cut(s) 1685
EaeI YGGCCR 3 cut(s) 89, 1633, 2109
Eam1104I CTCTTC 3 cut(s) 108, 549, 1875
EarI CTCTTC 3 cut(s) 108, 549, 1875
EciI GGCGGA 1 cut(s) 85
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 1014, 1491
Eco24I GRGCYC 2 cut(s) 110, 120
Eco31I GGTCTC 3 cut(s) 635, 823, 1175
Eco47I GGWCC 6 cut(s) 81, 153, 1442, 1560, 1687, 2148
Eco47III AGCGCT 1 cut(s) 1613
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 4 cut(s) 525, 612, 1473, 1491
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 2 cut(s) 81, 153
EcoRII CCWGG 5 cut(s) 83, 1279, 1562, 1689, 2043
EcoT14I CCWWGG 2 cut(s) 1014, 1491
EcoT22I ATGCAT 1 cut(s) 2199
EcoT38I GRGCYC 2 cut(s) 110, 120
ErhI CCWWGG 2 cut(s) 1014, 1491
Esp3I CGTCTC 1 cut(s) 510
FaqI GGGAC 4 cut(s) 166, 872, 1610, 1901
FauI CCCGC 1 cut(s) 25
FbaI TGATCA 2 cut(s) 583, 2232
Fnu4HI GCNGC 3 cut(s) 1364, 2109, 2256
FokI GGATG 6 cut(s) 619, 1139, 1211, 1424, 1786, 1958
FriOI GRGCYC 2 cut(s) 110, 120
Fsp4HI GCNGC 3 cut(s) 1364, 2109, 2256
FspBI CTAG 1 cut(s) 1341
GlaI GCGC 3 cut(s) 279, 342, 1613
GluI GCNGC 3 cut(s) 1364, 2109, 2256
HaeII RGCGCY 1 cut(s) 1615
HaeIII GGCC 6 cut(s) 91, 108, 269, 1635, 1657, 2111
HapII CCGG 5 cut(s) 92, 110, 1622, 2240, 2279
HgaI GACGC 1 cut(s) 1485
HhaI GCGC 3 cut(s) 280, 343, 1614
Hin1I GRCGYC 1 cut(s) 1496
Hin6I GCGC 3 cut(s) 278, 341, 1612
HinP1I GCGC 3 cut(s) 278, 341, 1612
HincII GTYRAC 2 cut(s) 193, 1678
HindII GTYRAC 2 cut(s) 193, 1678
HindIII AAGCTT 1 cut(s) 164
HpaII CCGG 5 cut(s) 92, 110, 1622, 2240, 2279
Hpy166II GTNNAC 3 cut(s) 193, 767, 1678
Hpy188III TCNNGA 7 cut(s) 206, 320, 826, 936, 1325, 1500, 2201
Hpy8I GTNNAC 3 cut(s) 193, 767, 1678
Hpy99I CGWCG 3 cut(s) 142, 321, 846
HpyAV CCTTC 6 cut(s) 259, 654, 1044, 1678, 2069, 2219
HpyCH4III ACNGT 7 cut(s) 680, 894, 911, 1446, 1526, 1822, 2266
HpyCH4IV ACGT 4 cut(s) 137, 316, 503, 841
HpyF10VI GCNNNNNNNGC 7 cut(s) 42, 275, 286, 881, 1346, 1502, 2055
HpySE526I ACGT 4 cut(s) 137, 316, 503, 841
Hsp92I GRCGYC 1 cut(s) 1496
HspAI GCGC 3 cut(s) 278, 341, 1612
KflI GGGWCCC 1 cut(s) 153
Ksp22I TGATCA 2 cut(s) 583, 2232
Kzo9I GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 6 cut(s) 456, 943, 1276, 1346, 1708, 2055
Lsp1109I GCAGC 2 cut(s) 1375, 2242
LweI GCATC 8 cut(s) 139, 172, 227, 316, 412, 1037, 1402, 2206
MaeI CTAG 1 cut(s) 1341
MaeII ACGT 4 cut(s) 137, 316, 503, 841
MaeIII GTNAC 5 cut(s) 792, 1697, 2030, 2183, 2301
MalI GATC 7 cut(s) 52, 56, 585, 1052, 2123, 2234, 2316
MboI GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
MfeI CAATTG 2 cut(s) 1719, 1845
MflI RGATCY 1 cut(s) 1050
MhlI GDGCHC 4 cut(s) 110, 120, 1075, 1767
MlyI GAGTC 7 cut(s) 1010, 1097, 1124, 1169, 1322, 1923, 2252
MmeI TCCRAC 3 cut(s) 1149, 1514, 1827
Mph1103I ATGCAT 1 cut(s) 2199
MroXI GAANNNNTTC 1 cut(s) 658
MseI TTAA 6 cut(s) 225, 674, 699, 806, 1742, 2004
MslI CAYNNNNRTG 6 cut(s) 235, 513, 759, 1299, 1879, 2279
MspA1I CMGCKG 2 cut(s) 998, 1366
MspI CCGG 5 cut(s) 92, 110, 1622, 2240, 2279
MspR9I CCNGG 8 cut(s) 85, 93, 110, 1281, 1564, 1622, 1691, 2045
MunI CAATTG 2 cut(s) 1719, 1845
Mva1269I GAATGC 2 cut(s) 1294, 2199
MvaI CCWGG 5 cut(s) 85, 1281, 1564, 1691, 2045
MvnI CGCG 1 cut(s) 726
MwoI GCNNNNNNNGC 7 cut(s) 42, 275, 286, 881, 1346, 1502, 2055
NciI CCSGG 3 cut(s) 93, 110, 1622
NcoI CCATGG 1 cut(s) 1491
NdeII GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
NlaIV GGNNCC 9 cut(s) 82, 108, 154, 155, 945, 1278, 1710, 1766, 2057
NmeAIII GCCGAG 2 cut(s) 150, 2053
NmuCI GTSAC 1 cut(s) 792
NsiI ATGCAT 1 cut(s) 2199
NspI RCATGY 1 cut(s) 1210
NspV TTCGAA 1 cut(s) 647
PagI TCATGA 2 cut(s) 1324, 2200
PciSI GCTCTTC 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 134
PctI GAATGC 2 cut(s) 1294, 2199
PdmI GAANNNNTTC 1 cut(s) 658
PfeI GAWTC 6 cut(s) 23, 272, 1010, 1136, 1172, 1260
PinAI ACCGGT 1 cut(s) 2278
PkrI GCNGC 3 cut(s) 1365, 2110, 2257
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 7 cut(s) 1009, 1096, 1124, 1168, 1322, 1922, 2251
PpsI GAGTC 7 cut(s) 1009, 1096, 1124, 1168, 1322, 1922, 2251
PpuMI RGGWCCY 2 cut(s) 81, 153
PshAI GACNNNNGTC 2 cut(s) 158, 627
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 2 cut(s) 81, 153
Psp6I CCWGG 5 cut(s) 83, 1279, 1562, 1689, 2043
PspGI CCWGG 5 cut(s) 83, 1279, 1562, 1689, 2043
PspN4I GGNNCC 9 cut(s) 82, 108, 154, 155, 945, 1278, 1710, 1766, 2057
PspOMI GGGCCC 1 cut(s) 106
PspPI GGNCC 8 cut(s) 81, 106, 107, 153, 1442, 1560, 1687, 2148
PspPPI RGGWCCY 2 cut(s) 81, 153
PsuI RGATCY 1 cut(s) 1050
PvuI CGATCG 1 cut(s) 57
PvuII CAGCTG 2 cut(s) 998, 1366
RsaI GTAC 3 cut(s) 200, 913, 1473
RsaNI GTAC 3 cut(s) 199, 912, 1472
RseI CAYNNNNRTG 6 cut(s) 235, 513, 759, 1299, 1879, 2279
SacI GAGCTC 1 cut(s) 120
SapI GCTCTTC 1 cut(s) 108
SaqAI TTAA 6 cut(s) 225, 674, 699, 806, 1742, 2004
SatI GCNGC 3 cut(s) 1364, 2109, 2256
Sau3AI GATC 7 cut(s) 50, 54, 583, 1050, 2121, 2232, 2314
Sau96I GGNCC 8 cut(s) 81, 106, 107, 153, 1442, 1560, 1687, 2148
ScaI AGTACT 1 cut(s) 913
SchI GAGTC 7 cut(s) 1010, 1097, 1124, 1169, 1322, 1923, 2252
ScrFI CCNGG 8 cut(s) 85, 93, 110, 1281, 1564, 1622, 1691, 2045
SduI GDGCHC 4 cut(s) 110, 120, 1075, 1767
SfaNI GCATC 8 cut(s) 139, 172, 227, 316, 412, 1037, 1402, 2206
SfcI CTRYAG 2 cut(s) 1318, 2265
SfuI TTCGAA 1 cut(s) 647
SinI GGWCC 6 cut(s) 81, 153, 1442, 1560, 1687, 2148
SmiMI CAYNNNNRTG 6 cut(s) 235, 513, 759, 1299, 1879, 2279
SmlI CTYRAG 2 cut(s) 350, 2306
SmoI CTYRAG 2 cut(s) 350, 2306
SsiI CCGC 7 cut(s) 20, 32, 70, 240, 726, 1387, 2108
SspI AATATT 1 cut(s) 871
SspMI CTAG 1 cut(s) 1341
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 8 cut(s) 83, 91, 108, 1279, 1562, 1620, 1689, 2043
StyI CCWWGG 2 cut(s) 1014, 1491
TaaI ACNGT 7 cut(s) 680, 894, 911, 1446, 1526, 1822, 2266
TaiI ACGT 4 cut(s) 140, 319, 506, 844
TaqI TCGA 5 cut(s) 53, 235, 319, 647, 1912
TaqII GACCGA 1 cut(s) 1199
TatI WGTACW 2 cut(s) 911, 1471
TauI GCSGC 1 cut(s) 2111
TfiI GAWTC 6 cut(s) 23, 272, 1010, 1136, 1172, 1260
Tru1I TTAA 6 cut(s) 225, 674, 699, 806, 1742, 2004
Tru9I TTAA 6 cut(s) 225, 674, 699, 806, 1742, 2004
TscAI CASTG 3 cut(s) 193, 1531, 2089
TseFI GTSAC 1 cut(s) 792
TseI GCWGC 2 cut(s) 1363, 2255
Tsp45I GTSAC 1 cut(s) 792
TspGWI ACGGA 2 cut(s) 1437, 2188
TspRI CASTG 3 cut(s) 193, 1531, 2089
VpaK11BI GGWCC 6 cut(s) 81, 153, 1442, 1560, 1687, 2148
XapI RAATTY 5 cut(s) 487, 657, 816, 1829, 2176
XceI RCATGY 1 cut(s) 1210
XmnI GAANNNNTTC 1 cut(s) 658
XspI CTAG 1 cut(s) 1341
ZrmI AGTACT 1 cut(s) 913
Zsp2I ATGCAT 1 cut(s) 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.