pycom08g02730

Protein of unknown function (DUF1195)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0014678
Physical Location & Seq
Forward (+)
1989959 .. 1990647
689 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGAAGGACAACGATGCACCAACACTGATAACGATCCCATCATCAACGGCGATCAGGAAGGACGCCTCATCGTCGTCATCATCCTCGAAAACCAGAGTCTTTCTCGACAAAAATGGCTACAAGTTCTGGGCTTTAGCGGCGATTCTTCTCCTTGCTTTCTGGTCCATGTTCACCGGCTCCGTCACCCTCAAATGGTCCACCGGCAACTTGTCCCGCCTCTCCGACGACCTCGACATCCCGACCTTCGACGATCTCGACATTCTGGAGGTGGAGGAGAGAGAGAAGGTGGTGAGGCACATGTGGGATTTGTACACGCAGACCACCAGCACCAGCACGAGTCGATTGCCGCGGTTTTGGCAGGAGGCTTTCGAGGCGGCGTACGAGCACTTGACCAGCGATGTTGCCGCCGTTCGAGACGCCGCCGTTTCGGAGATTGCCAAGATGTCCATCCGCTCCATCGTTCTTGATCCCTTTCCTCTTCAATCAACGGTCAGTAAGCCCTCTGATCTGCATAATCAAATCTCTTTTATGTTAATGGGTGATCATTTTTCTGCTAAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1195

Protein Analysis

188

Amino Acids

21.02

Weight (kDa)

5.33

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1195 PF06708 21 - 157 2.6e-65 Protein of unknown function (DUF1195)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 453
AccII CGCG 1 cut(s) 349
AciI CCGC 8 cut(s) 137, 214, 347, 349, 374, 405, 420, 451
AclWI GGATC 2 cut(s) 28, 461
AcsI RAATTY 1 cut(s) 557
AcyI GRCGYC 2 cut(s) 63, 417
AfaI GTAC 2 cut(s) 311, 380
AfiI CCNNNNNNNGG 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 1 cut(s) 482
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 2 cut(s) 28, 461
ApoI RAATTY 1 cut(s) 557
AspS9I GGNCC 2 cut(s) 162, 195
AsuHPI GGTGA 4 cut(s) 163, 175, 301, 551
AvaII GGWCC 2 cut(s) 162, 195
BauI CACGAG 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 387
BccI CCATC 3 cut(s) 46, 455, 464
BceAI ACGGC 3 cut(s) 63, 392, 407
BcgI CGANNNNNNTGC 2 cut(s) 325, 359
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 408
BisI GCNGC 5 cut(s) 138, 347, 375, 405, 420
BlsI GCNGC 5 cut(s) 139, 348, 376, 406, 421
Bme18I GGWCC 2 cut(s) 162, 195
BmgT120I GGNCC 2 cut(s) 162, 195
BmiI GGNNCC 1 cut(s) 178
BmsI GCATC 1 cut(s) 4
BplI GAGNNNNNCTC 2 cut(s) 87, 119
BpmI CTGGAG 1 cut(s) 284
BsaBI GATNNNNATC 2 cut(s) 32, 446
BsaHI GRCGYC 2 cut(s) 63, 417
BsaJI CCNNGG 1 cut(s) 347
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse118I RCCGGY 2 cut(s) 173, 200
Bse8I GATNNNNATC 2 cut(s) 32, 446
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 3 cut(s) 80, 234, 447
BseJI GATNNNNATC 2 cut(s) 32, 446
BseLI CCNNNNNNNGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 349
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 2 cut(s) 174, 201
BsiWI CGTACG 1 cut(s) 378
BslFI GGGAC 1 cut(s) 196
BslI CCNNNNNNNGG 1 cut(s) 192
BsmAI GTCTC 1 cut(s) 408
BsmBI CGTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 387
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 6 cut(s) 33, 51, 250, 466, 505, 541
BspACI CCGC 8 cut(s) 137, 214, 347, 349, 374, 405, 420, 451
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 178
BspPI GGATC 2 cut(s) 28, 461
BsrBI CCGCTC 1 cut(s) 453
BsrFI RCCGGY 2 cut(s) 173, 200
BsrGI TGTACA 1 cut(s) 309
BssAI RCCGGY 2 cut(s) 173, 200
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 6 cut(s) 33, 51, 250, 466, 505, 541
BssNI GRCGYC 2 cut(s) 63, 417
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 1 cut(s) 483
BstACI GRCGYC 2 cut(s) 63, 417
BstAUI TGTACA 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 3 cut(s) 80, 234, 447
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 6 cut(s) 36, 54, 253, 469, 508, 544
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 6 cut(s) 33, 51, 250, 466, 505, 541
BstMWI GCNNNNNNNGC 3 cut(s) 137, 355, 371
BstNSI RCATGY 1 cut(s) 301
BstUI CGCG 1 cut(s) 349
BtgI CCRYGG 1 cut(s) 347
BtgZI GCGATG 1 cut(s) 411
BtsCI GGATG 3 cut(s) 80, 234, 447
BtsIMutI CAGTG 1 cut(s) 23
Cfr10I RCCGGY 2 cut(s) 173, 200
Cfr13I GGNCC 2 cut(s) 162, 195
Cfr42I CCGCGG 1 cut(s) 350
CseI GACGC 2 cut(s) 71, 425
Csp6I GTAC 2 cut(s) 310, 379
CviAII CATG 2 cut(s) 166, 298
CviJI RGCY 5 cut(s) 117, 131, 177, 365, 499
CviKI_1 RGCY 5 cut(s) 117, 131, 177, 365, 499
CviQI GTAC 2 cut(s) 310, 379
DpnI GATC 6 cut(s) 35, 53, 252, 468, 507, 543
DpnII GATC 6 cut(s) 33, 51, 250, 466, 505, 541
Eam1104I CTCTTC 1 cut(s) 483
EarI CTCTTC 1 cut(s) 483
Eco47I GGWCC 2 cut(s) 162, 195
Esp3I CGTCTC 1 cut(s) 408
FaeI CATG 2 cut(s) 169, 301
FaiI YATR 4 cut(s) 167, 299, 513, 530
FaqI GGGAC 1 cut(s) 196
FatI CATG 2 cut(s) 165, 297
FauI CCCGC 1 cut(s) 221
FbaI TGATCA 1 cut(s) 541
Fnu4HI GCNGC 5 cut(s) 138, 347, 375, 405, 420
FokI GGATG 3 cut(s) 67, 221, 434
Fsp4HI GCNGC 5 cut(s) 138, 347, 375, 405, 420
GluI GCNGC 5 cut(s) 138, 347, 375, 405, 420
GsuI CTGGAG 1 cut(s) 284
HapII CCGG 2 cut(s) 174, 201
HgaI GACGC 2 cut(s) 71, 425
Hin1I GRCGYC 2 cut(s) 63, 417
Hin1II CATG 2 cut(s) 169, 301
HinfI GANTC 3 cut(s) 96, 142, 337
HpaII CCGG 2 cut(s) 174, 201
HphI GGTGA 4 cut(s) 163, 175, 301, 551
Hpy166II GTNNAC 3 cut(s) 171, 198, 312
Hpy188I TCNGA 3 cut(s) 223, 430, 505
Hpy188III TCNNGA 7 cut(s) 55, 104, 238, 254, 263, 413, 464
Hpy8I GTNNAC 3 cut(s) 171, 198, 312
Hpy99I CGWCG 3 cut(s) 76, 227, 251
HpyAV CCTTC 3 cut(s) 52, 253, 277
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 2 cut(s) 17, 511
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 355, 371
Hsp92I GRCGYC 2 cut(s) 63, 417
Hsp92II CATG 2 cut(s) 169, 301
Ksp22I TGATCA 1 cut(s) 541
KspI CCGCGG 1 cut(s) 350
Kzo9I GATC 6 cut(s) 33, 51, 250, 466, 505, 541
LmnI GCTCC 2 cut(s) 182, 458
LweI GCATC 1 cut(s) 4
MaeIII GTNAC 1 cut(s) 181
MalI GATC 6 cut(s) 35, 53, 252, 468, 507, 543
MbiI CCGCTC 1 cut(s) 453
MboI GATC 6 cut(s) 33, 51, 250, 466, 505, 541
MboII GAAGA 2 cut(s) 137, 470
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 1 cut(s) 557
MlyI GAGTC 2 cut(s) 105, 346
MmeI TCCRAC 1 cut(s) 246
MseI TTAA 2 cut(s) 533, 562
MspA1I CMGCKG 1 cut(s) 349
MspI CCGG 2 cut(s) 174, 201
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 137, 355, 371
NdeII GATC 6 cut(s) 33, 51, 250, 466, 505, 541
NlaIII CATG 2 cut(s) 169, 301
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 181
NspI RCATGY 1 cut(s) 301
PciI ACATGT 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PfeI GAWTC 1 cut(s) 142
Pfl23II CGTACG 1 cut(s) 378
PkrI GCNGC 5 cut(s) 139, 348, 376, 406, 421
PleI GAGTC 2 cut(s) 104, 345
PpsI GAGTC 2 cut(s) 104, 345
PscI ACATGT 1 cut(s) 297
PspLI CGTACG 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 2 cut(s) 162, 195
RsaI GTAC 2 cut(s) 311, 380
RsaNI GTAC 2 cut(s) 310, 379
SacII CCGCGG 1 cut(s) 350
SaqAI TTAA 2 cut(s) 533, 562
SatI GCNGC 5 cut(s) 138, 347, 375, 405, 420
Sau3AI GATC 6 cut(s) 33, 51, 250, 466, 505, 541
Sau96I GGNCC 2 cut(s) 162, 195
SchI GAGTC 2 cut(s) 105, 346
SduI GDGCHC 1 cut(s) 387
SetI ASST 4 cut(s) 231, 245, 270, 288
SfaNI GCATC 1 cut(s) 4
Sfr303I CCGCGG 1 cut(s) 350
SgrBI CCGCGG 1 cut(s) 350
SinI GGWCC 2 cut(s) 162, 195
Sse9I AATT 1 cut(s) 557
SsiI CCGC 8 cut(s) 137, 214, 347, 349, 374, 405, 420, 451
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 8 cut(s) 86, 105, 231, 246, 255, 340, 369, 412
TasI AATT 1 cut(s) 557
TatI WGTACW 1 cut(s) 309
TauI GCSGC 5 cut(s) 140, 349, 377, 407, 422
TfiI GAWTC 1 cut(s) 142
Tru1I TTAA 2 cut(s) 533, 562
Tru9I TTAA 2 cut(s) 533, 562
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 181
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 1 cut(s) 30
VpaK11BI GGWCC 2 cut(s) 162, 195
XapI RAATTY 1 cut(s) 557
XceI RCATGY 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.