pycom08g03490

K( ) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0015721
Physical Location & Seq
Forward (+)
2739685 .. 2740699
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g03490.2

Sequence Viewer

Length: 435 bp
ATGCTAATCCATGTTCAATTTCTTTGGAACCACGTCGATATATTACTAGCATCTGTTATATTAGTAATCATCATAAAAACAATTATTATCACCATGGTTGTCAAGGGATTTGGTTACAACAACAAAGCATCATTTCTTGTTGGAATTTCTCTGGCACAGATAGGCGAATTTGCTTTTGTTCTTCTAAGTCGCGCCTCTAATCTCCATCTTGTTGAGGGGAAATTATATCTGCTGCTTCTTGGAACCACAGCACTTAGTCTGGTGACCACTCCTTTTCTATTCAAGCTAATTCCTGGTTTAGTGCATCTTGGTGTGCTTTTAAGATGGTTCCCTCCCGATGGTGCTGTTGAGATTGGGTTTAAAGGAGAAAACCTTCGCTCAGAAAGTGGCAAACAACGCGTTATTTTGATGGTCCGAGAATCACATGATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

145

Amino Acids

15.93

Weight (kDa)

9.45

Isoelectric Point (pI)

29.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 192, 399
AcsI RAATTY 2 cut(s) 144, 167
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 397
AgsI TTSAA 2 cut(s) 17, 283
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 1 cut(s) 286
AluI AGCT 1 cut(s) 286
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 2 cut(s) 144, 167
Asp700I GAANNNNTTC 1 cut(s) 372
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 412
AsuHPI GGTGA 2 cut(s) 82, 274
AvaII GGWCC 1 cut(s) 412
BbvI GCAGC 1 cut(s) 219
BccI CCATC 4 cut(s) 213, 318, 332, 403
BciT130I CCWGG 1 cut(s) 294
BfaI CTAG 1 cut(s) 47
BisI GCNGC 1 cut(s) 233
BlsI GCNGC 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 1 cut(s) 412
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 3 cut(s) 29, 244, 329
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 3 cut(s) 59, 137, 313
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 338
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 1 cut(s) 393
BseXI GCAGC 1 cut(s) 219
Bsh1236I CGCG 2 cut(s) 192, 399
BslI CCNNNNNNNGG 1 cut(s) 338
Bsp19I CCATGG 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 392
BspFNI CGCG 2 cut(s) 192, 399
BspLI GGNNCC 3 cut(s) 29, 244, 329
BssECI CCNNGG 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 93
Bst2UI CCWGG 1 cut(s) 294
BstDEI CTNAG 3 cut(s) 185, 254, 379
BstDSI CCRYGG 1 cut(s) 93
BstEII GGTNACC 1 cut(s) 262
BstFNI CGCG 2 cut(s) 192, 399
BstHHI GCGC 1 cut(s) 194
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstNI CCWGG 1 cut(s) 294
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 292
BstUI CGCG 2 cut(s) 192, 399
BstV1I GCAGC 1 cut(s) 219
BtgI CCRYGG 1 cut(s) 93
BtrI CACGTC 1 cut(s) 34
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 412
CviAII CATG 3 cut(s) 11, 94, 425
CviJI RGCY 1 cut(s) 286
CviKI_1 RGCY 1 cut(s) 286
DdeI CTNAG 3 cut(s) 185, 254, 379
DraI TTTAAA 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 93
Eco47I GGWCC 1 cut(s) 412
Eco91I GGTNACC 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 3 cut(s) 14, 97, 428
FaiI YATR 7 cut(s) 12, 41, 59, 74, 95, 226, 426
FatI CATG 3 cut(s) 10, 93, 424
Fnu4HI GCNGC 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 233
FspBI CTAG 1 cut(s) 47
GlaI GCGC 1 cut(s) 193
GluI GCNGC 1 cut(s) 233
HhaI GCGC 1 cut(s) 194
Hin1II CATG 3 cut(s) 14, 97, 428
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HinfI GANTC 2 cut(s) 419, 428
HphI GGTGA 2 cut(s) 82, 274
Hpy188I TCNGA 2 cut(s) 382, 416
Hpy188III TCNNGA 2 cut(s) 335, 432
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 1 cut(s) 383
HpyCH4IV ACGT 1 cut(s) 33
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 3 cut(s) 185, 254, 379
HpySE526I ACGT 1 cut(s) 33
Hsp92II CATG 3 cut(s) 14, 97, 428
HspAI GCGC 1 cut(s) 192
LpnPI CCDG 4 cut(s) 137, 245, 279, 306
Lsp1109I GCAGC 1 cut(s) 219
LweI GCATC 3 cut(s) 59, 137, 313
MaeI CTAG 1 cut(s) 47
MaeII ACGT 1 cut(s) 33
MaeIII GTNAC 2 cut(s) 113, 262
MboII GAAGA 1 cut(s) 173
MluCI AATT 6 cut(s) 17, 81, 144, 167, 221, 288
MluI ACGCGT 1 cut(s) 397
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 3 cut(s) 205, 208, 342
MroXI GAANNNNTTC 1 cut(s) 372
MseI TTAA 2 cut(s) 320, 360
MslI CAYNNNNRTG 1 cut(s) 309
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MvnI CGCG 2 cut(s) 192, 399
MwoI GCNNNNNNNGC 1 cut(s) 396
NcoI CCATGG 1 cut(s) 93
NlaIII CATG 3 cut(s) 14, 97, 428
NlaIV GGNNCC 3 cut(s) 29, 244, 329
NmuCI GTSAC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 419, 428
PkrI GCNGC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 292
PspEI GGTNACC 1 cut(s) 262
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 3 cut(s) 29, 244, 329
PspPI GGNCC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 309
SaqAI TTAA 2 cut(s) 320, 360
SatI GCNGC 1 cut(s) 233
Sau96I GGNCC 1 cut(s) 412
ScrFI CCNGG 1 cut(s) 294
SetI ASST 3 cut(s) 36, 288, 375
SfaNI GCATC 3 cut(s) 59, 137, 313
SinI GGWCC 1 cut(s) 412
SmiMI CAYNNNNRTG 1 cut(s) 309
Sse9I AATT 6 cut(s) 17, 81, 144, 167, 221, 288
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 93
TaiI ACGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 36
TasI AATT 6 cut(s) 17, 81, 144, 167, 221, 288
TfiI GAWTC 2 cut(s) 419, 428
Tru1I TTAA 2 cut(s) 320, 360
Tru9I TTAA 2 cut(s) 320, 360
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 412
XapI RAATTY 2 cut(s) 144, 167
XmnI GAANNNNTTC 1 cut(s) 372
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.