pycom08g08530

carboxylesterase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011899
Physical Location & Seq
Forward (+)
6814428 .. 6815585
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1158 bp
ATGTCACATGCCGCATGTGTATGGTACAACCATATACAATTCCATTCCCTCACACTCTTCTCATCCCCCTCCATAAAATGCATGTCAACTCAAGTCATTCTCCAACGTCCAAAGCCTCCATCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCCCCTCTCCTCCTTGTTTCTCTTCCATACAAATGGCTGCAACTACCTCACCGGAAGCGTTACTTGATGTCCTTCCATACCTCCGAGTCCTCAAAGATGGCACAATCGACAGACTCGCCGGAACCGAAGTCGCTCCTCCCGGCCTCGACCCCAAAACCGGAGTTTTATCCAAAGACATCGTCATCTTCCCCAAGACCGGCGTGTCTGCCCGACTTTACCGACCCAACACGGCCAAACCCCCCCATAAACTTCCGCTCATCATATACTTCCACGGTGGAGCCTTCTGCATAGCTTCCGCTGGCGAGCCGCTTTACCACACCAGCCTCAACAACCTTGTTGCGGAAGCCAACGCCATAGGGGTTTCAGTAAACTACAGATTAGCACCTGAGCACCCGCTCCCCACCGCTTACGAAGACTGCTGGGCCGTCCTCAACTGGGTTTTCAACGGCGGCAAAGGTCGTGATTCATGGGTCAAGGACCACGTCGATTTCGAGCGCGTGTTTTTGGTCGGAGACAGCGCAGGGGCCAACATTGCACACCACTTGGCCTTACGGATCAAGCCCTCCGATCCGGATCCGAAATTGAAGATAGCCGGGATTGGTATGATCAATCCATACTTTTGGGGGAAGGAGCCAATTGGTGGGGAGGTGGGGGATTTGGTGAGGAAGTCCATGGTGGACACGTGGTGGAATTTCGTTTGCCCGTCGGAGAAAGGCTGCGACGACCCGCTTATCAACCCGTTTGTGGGCGGATCGCCGGGGTTGGAGGGGTTGGCTTGTGACAAAATACTTGTTTTGGTGGCAGGAAAAGATATATTGAGGGATAGAGGGATGCTTTACTATTATGAGTTGGTGAAGAGCAAATGGGGAGGGAGGAAGGAGTTGATTGAAACACAAGGGGAGGATCATGTTTTTCATATCTTTAATCCCAATTGTGACAAGGCTAAGATCTTGATTAGGAATTTGGGTAAGTTCATTAATCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

386

Amino Acids

42.28

Weight (kDa)

7.61

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 128 - 240 3.4e-12 BD-FAE
COesterase PF00135 129 - 240 7.2e-07 Carboxylesterase family
Abhydrolase_3 PF07859 143 - 362 1e-53 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 370
AccB7I CCANNNNNTGG 1 cut(s) 809
AccBSI CCGCTC 2 cut(s) 424, 565
AccII CGCG 1 cut(s) 666
AccIII TCCGGA 1 cut(s) 739
AclWI GGATC 6 cut(s) 731, 731, 737, 750, 928, 1080
AcoI YGGCCR 1 cut(s) 399
AcsI RAATTY 2 cut(s) 859, 1129
AcvI CACGTG 1 cut(s) 852
AdeI CACNNNGTG 1 cut(s) 855
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 7 cut(s) 326, 365, 508, 604, 809, 913, 914
AflIII ACRYGT 1 cut(s) 849
AgsI TTSAA 3 cut(s) 613, 754, 1058
AjiI CACGTC 1 cut(s) 652
AluBI AGCT 1 cut(s) 461
AluI AGCT 1 cut(s) 461
Alw21I GWGCWC 1 cut(s) 561
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 6 cut(s) 731, 731, 737, 750, 928, 1080
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 5 cut(s) 310, 399, 591, 693, 714
ApeKI GCWGC 2 cut(s) 206, 885
ApoI RAATTY 2 cut(s) 859, 1129
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
AseI ATTAAT 1 cut(s) 1146
AspLEI GCGC 2 cut(s) 666, 689
AspS9I GGNCC 3 cut(s) 591, 646, 693
AsuC2I CCSGG 3 cut(s) 309, 763, 927
AsuHPI GGTGA 3 cut(s) 210, 841, 1033
AvaII GGWCC 1 cut(s) 646
BamHI GGATCC 1 cut(s) 742
BbrPI CACGTG 1 cut(s) 852
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 2 cut(s) 193, 872
BccI CCATC 2 cut(s) 127, 260
BceAI ACGGC 3 cut(s) 414, 578, 631
BclI TGATCA 1 cut(s) 774
BcnI CCSGG 3 cut(s) 309, 763, 927
BcoDI GTCTC 1 cut(s) 675
BfmI CTRYAG 1 cut(s) 541
BglII AGATCT 1 cut(s) 1116
BisI GCNGC 5 cut(s) 12, 207, 476, 619, 886
BlsI GCNGC 5 cut(s) 13, 208, 477, 620, 887
Bme1390I CCNGG 3 cut(s) 309, 763, 927
Bme18I GGWCC 1 cut(s) 646
BmgBI CACGTC 1 cut(s) 652
BmgT120I GGNCC 3 cut(s) 591, 646, 693
BmiI GGNNCC 5 cut(s) 292, 448, 694, 744, 801
BmrFI CCNGG 3 cut(s) 309, 763, 927
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 1 cut(s) 990
BmuI ACTGGG 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 588
Bpu10I CCTNAGC 1 cut(s) 555
BpuEI CTTGAG 1 cut(s) 75
BpuMI CCSGG 3 cut(s) 309, 763, 927
BsaAI YACGTR 1 cut(s) 852
BsaBI GATNNNNATC 1 cut(s) 741
BsaJI CCNNGG 3 cut(s) 439, 840, 926
BsaWI WCCGGW 3 cut(s) 220, 326, 739
Bsc4I CCNNNNNNNGG 7 cut(s) 326, 365, 508, 604, 809, 913, 914
Bse118I RCCGGY 1 cut(s) 365
Bse1I ACTGG 1 cut(s) 608
Bse3DI GCAATG 1 cut(s) 699
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 739
BseDI CCNNGG 3 cut(s) 439, 840, 926
BseGI GGATG 2 cut(s) 62, 1005
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 7 cut(s) 326, 365, 508, 604, 809, 913, 914
BseMI GCAATG 1 cut(s) 699
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 2 cut(s) 168, 294
BseXI GCAGC 2 cut(s) 193, 872
BseYI CCCAGC 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 5 cut(s) 312, 401, 593, 695, 716
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 8 cut(s) 221, 288, 309, 327, 366, 740, 762, 926
BslI CCNNNNNNNGG 7 cut(s) 326, 365, 508, 604, 809, 913, 914
BsmAI GTCTC 1 cut(s) 675
BsnI GGCC 5 cut(s) 312, 401, 593, 695, 716
Bsp1286I GDGCHC 1 cut(s) 561
Bsp13I TCCGGA 1 cut(s) 739
Bsp143I GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
Bsp19I CCATGG 1 cut(s) 840
BspANI GGCC 5 cut(s) 312, 401, 593, 695, 716
BspCNI CTCAG 1 cut(s) 547
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 5 cut(s) 292, 448, 694, 744, 801
BspPI GGATC 6 cut(s) 731, 731, 737, 750, 928, 1080
BspQI GCTCTTC 1 cut(s) 1019
BsrBI CCGCTC 2 cut(s) 424, 565
BsrDI GCAATG 1 cut(s) 699
BsrFI RCCGGY 1 cut(s) 365
BsrI ACTGG 1 cut(s) 608
BssAI RCCGGY 1 cut(s) 365
BssECI CCNNGG 3 cut(s) 439, 840, 926
BssMI GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
BssT1I CCWWGG 1 cut(s) 840
Bst4CI ACNGT 1 cut(s) 443
Bst6I CTCTTC 3 cut(s) 62, 196, 1019
BstBAI YACGTR 1 cut(s) 852
BstC8I GCNNGC 2 cut(s) 469, 473
BstDEI CTNAG 2 cut(s) 555, 1113
BstDSI CCRYGG 2 cut(s) 439, 840
BstF5I GGATG 2 cut(s) 62, 1005
BstFNI CGCG 1 cut(s) 666
BstHHI GCGC 2 cut(s) 666, 689
BstKTI GATC 7 cut(s) 726, 739, 745, 777, 923, 1075, 1119
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
BstMWI GCNNNNNNNGC 1 cut(s) 701
BstNSI RCATGY 3 cut(s) 11, 18, 85
BstSCI CCNGG 3 cut(s) 307, 761, 925
BstSFI CTRYAG 1 cut(s) 541
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 2 cut(s) 193, 872
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 2 cut(s) 742, 1116
BstXI CCANNNNNNTGG 2 cut(s) 202, 789
BstYI RGATCY 2 cut(s) 742, 1116
BsuRI GGCC 5 cut(s) 312, 401, 593, 695, 716
BtgI CCRYGG 2 cut(s) 439, 840
BtrI CACGTC 1 cut(s) 652
BtsCI GGATG 2 cut(s) 62, 1005
Cac8I GCNNGC 2 cut(s) 469, 473
CfoI GCGC 2 cut(s) 666, 689
Cfr10I RCCGGY 1 cut(s) 365
Cfr13I GGNCC 3 cut(s) 591, 646, 693
Csp6I GTAC 1 cut(s) 25
CviAII CATG 6 cut(s) 8, 15, 82, 636, 841, 1076
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 555, 1113
DpnI GATC 7 cut(s) 725, 738, 744, 776, 922, 1074, 1118
DpnII GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
DraIII CACNNNGTG 1 cut(s) 855
DrdI GACNNNNNNGTC 1 cut(s) 370
DseDI GACNNNNNNGTC 1 cut(s) 370
EaeI YGGCCR 1 cut(s) 399
Eam1104I CTCTTC 3 cut(s) 62, 196, 1019
EarI CTCTTC 3 cut(s) 62, 196, 1019
EciI GGCGGA 1 cut(s) 933
Eco130I CCWWGG 1 cut(s) 840
Eco47I GGWCC 1 cut(s) 646
Eco72I CACGTG 1 cut(s) 852
EcoT14I CCWWGG 1 cut(s) 840
EcoT22I ATGCAT 1 cut(s) 83
ErhI CCWWGG 1 cut(s) 840
FaeI CATG 6 cut(s) 11, 18, 85, 639, 844, 1079
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FatI CATG 6 cut(s) 7, 14, 81, 635, 840, 1075
FauI CCCGC 2 cut(s) 570, 903
FbaI TGATCA 1 cut(s) 774
Fnu4HI GCNGC 5 cut(s) 12, 207, 476, 619, 886
FokI GGATG 2 cut(s) 49, 1012
Fsp4HI GCNGC 5 cut(s) 12, 207, 476, 619, 886
GlaI GCGC 2 cut(s) 665, 688
GluI GCNGC 5 cut(s) 12, 207, 476, 619, 886
GsaI CCCAGC 1 cut(s) 592
HaeIII GGCC 5 cut(s) 312, 401, 593, 695, 716
HapII CCGG 8 cut(s) 221, 288, 309, 327, 366, 740, 762, 926
HhaI GCGC 2 cut(s) 666, 689
Hin1II CATG 6 cut(s) 11, 18, 85, 639, 844, 1079
Hin6I GCGC 2 cut(s) 664, 687
HinP1I GCGC 2 cut(s) 664, 687
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HinfI GANTC 3 cut(s) 255, 282, 632
HpaII CCGG 8 cut(s) 221, 288, 309, 327, 366, 740, 762, 926
HphI GGTGA 3 cut(s) 210, 841, 1033
Hpy166II GTNNAC 3 cut(s) 87, 538, 847
Hpy188I TCNGA 5 cut(s) 254, 680, 736, 747, 877
Hpy188III TCNNGA 4 cut(s) 629, 740, 1120, 1151
Hpy8I GTNNAC 3 cut(s) 87, 538, 847
Hpy99I CGWCG 3 cut(s) 656, 877, 893
HpyAV CCTTC 4 cut(s) 251, 460, 790, 1039
HpyCH4III ACNGT 1 cut(s) 443
HpyCH4IV ACGT 3 cut(s) 106, 651, 851
HpyCH4V TGCA 4 cut(s) 81, 209, 456, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 701
HpyF3I CTNAG 2 cut(s) 555, 1113
HpySE526I ACGT 3 cut(s) 106, 651, 851
Hsp92II CATG 6 cut(s) 11, 18, 85, 639, 844, 1079
HspAI GCGC 2 cut(s) 664, 687
Kpn2I TCCGGA 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 774
Kzo9I GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
LguI GCTCTTC 1 cut(s) 1019
LmnI GCTCC 4 cut(s) 307, 446, 570, 799
Lsp1109I GCAGC 2 cut(s) 193, 872
LweI GCATC 1 cut(s) 990
MaeII ACGT 3 cut(s) 106, 651, 851
MaeIII GTNAC 4 cut(s) 3, 228, 947, 1103
MalI GATC 7 cut(s) 725, 738, 744, 776, 922, 1074, 1118
MbiI CCGCTC 2 cut(s) 424, 565
MboI GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
MboII GAAGA 6 cut(s) 49, 183, 346, 593, 766, 1036
MfeI CAATTG 2 cut(s) 804, 1099
MflI RGATCY 2 cut(s) 742, 1116
MhlI GDGCHC 1 cut(s) 561
MluCI AATT 6 cut(s) 38, 749, 804, 859, 1099, 1129
MlyI GAGTC 2 cut(s) 264, 276
MmeI TCCRAC 4 cut(s) 127, 658, 855, 912
Mph1103I ATGCAT 1 cut(s) 83
MroI TCCGGA 1 cut(s) 739
MseI TTAA 3 cut(s) 1092, 1146, 1156
MslI CAYNNNNRTG 2 cut(s) 19, 200
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 8 cut(s) 221, 288, 309, 327, 366, 740, 762, 926
MspR9I CCNGG 3 cut(s) 309, 763, 927
MunI CAATTG 2 cut(s) 804, 1099
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 1 cut(s) 701
NciI CCSGG 3 cut(s) 309, 763, 927
NcoI CCATGG 1 cut(s) 840
NdeII GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
NlaIII CATG 6 cut(s) 11, 18, 85, 639, 844, 1079
NlaIV GGNNCC 5 cut(s) 292, 448, 694, 744, 801
NmuCI GTSAC 3 cut(s) 3, 947, 1103
NsiI ATGCAT 1 cut(s) 83
NspI RCATGY 3 cut(s) 11, 18, 85
PciSI GCTCTTC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 657
PfeI GAWTC 1 cut(s) 632
PflFI GACNNNGTC 2 cut(s) 347, 650
PflMI CCANNNNNTGG 1 cut(s) 809
PkrI GCNGC 5 cut(s) 13, 208, 477, 620, 887
PleI GAGTC 2 cut(s) 263, 276
PmaCI CACGTG 1 cut(s) 852
PmlI CACGTG 1 cut(s) 852
PpsI GAGTC 2 cut(s) 263, 276
Ppu21I YACGTR 1 cut(s) 852
PshBI ATTAAT 1 cut(s) 1146
PspCI CACGTG 1 cut(s) 852
PspFI CCCAGC 1 cut(s) 588
PspN4I GGNNCC 5 cut(s) 292, 448, 694, 744, 801
PspPI GGNCC 3 cut(s) 591, 646, 693
PsuI RGATCY 2 cut(s) 742, 1116
PsyI GACNNNGTC 2 cut(s) 347, 650
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
RseI CAYNNNNRTG 2 cut(s) 19, 200
SapI GCTCTTC 1 cut(s) 1019
SaqAI TTAA 3 cut(s) 1092, 1146, 1156
SatI GCNGC 5 cut(s) 12, 207, 476, 619, 886
Sau3AI GATC 7 cut(s) 723, 736, 742, 774, 920, 1072, 1116
Sau96I GGNCC 3 cut(s) 591, 646, 693
SchI GAGTC 2 cut(s) 264, 276
ScrFI CCNGG 3 cut(s) 309, 763, 927
SduI GDGCHC 1 cut(s) 561
SfaNI GCATC 1 cut(s) 990
SfcI CTRYAG 1 cut(s) 541
SinI GGWCC 1 cut(s) 646
SmiMI CAYNNNNRTG 2 cut(s) 19, 200
SmlI CTYRAG 1 cut(s) 90
SmoI CTYRAG 1 cut(s) 90
Sse9I AATT 6 cut(s) 38, 749, 804, 859, 1099, 1129
StyD4I CCNGG 3 cut(s) 307, 761, 925
StyI CCWWGG 1 cut(s) 840
TaaI ACNGT 1 cut(s) 443
TaiI ACGT 3 cut(s) 109, 654, 854
TaqI TCGA 4 cut(s) 276, 315, 654, 660
TasI AATT 6 cut(s) 38, 749, 804, 859, 1099, 1129
TauI GCSGC 3 cut(s) 14, 478, 621
TfiI GAWTC 1 cut(s) 632
Tru1I TTAA 3 cut(s) 1092, 1146, 1156
Tru9I TTAA 3 cut(s) 1092, 1146, 1156
TseFI GTSAC 3 cut(s) 3, 947, 1103
TseI GCWGC 2 cut(s) 206, 885
Tsp45I GTSAC 3 cut(s) 3, 947, 1103
TspDTI ATGAA 3 cut(s) 624, 1073, 1132
TspGWI ACGGA 1 cut(s) 736
Tth111I GACNNNGTC 2 cut(s) 347, 650
Van91I CCANNNNNTGG 1 cut(s) 809
VpaK11BI GGWCC 1 cut(s) 646
VspI ATTAAT 1 cut(s) 1146
XapI RAATTY 2 cut(s) 859, 1129
XceI RCATGY 3 cut(s) 11, 18, 85
Zsp2I ATGCAT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.