pycom08g09100

Oleosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007663
Physical Location & Seq
Reverse (-)
7353582 .. 7353980
399 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 399 bp
ATGGCCGATCAATCACGCCAGCAACAACTGACCCGAAAGCTCCACGACCAAGACCAGGACCAGTGGCAGCTGTTGGCCCCCTCCTCCTCCGCAGCCGGCCAGACTGGCAAGTTCCTGACCGCGGGCGCAGCTGGTATCACGCTCTTGATGTTATCCGGGTTAACTTTAACCGGGACAGTGATGGCCCTCATCATGGCCACCCCGGTTTTGGTCCTGTTTAGTCCGGTTCTAGTACCGGCTGCGATTGTCCTGTTCCTGACGGCAGCTGGTCTTGTTTTCTCCGGCGGGTGCGGCATGGCAGCAATACTGGCGTTTTCGTGGATGTATAAGAATGTGTCGAGTTACGTGGCTGCTACGAAAAGGGGTAACCCGTATGGGCAGTTCGCACAAATTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oleosin

Protein Analysis

133

Amino Acids

13.82

Weight (kDa)

9.36

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 36 - 114 1.1e-28 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 122
AciI CCGC 5 cut(s) 90, 120, 122, 285, 291
AcoI YGGCCR 3 cut(s) 3, 97, 195
AfaI GTAC 1 cut(s) 234
AfiI CCNNNNNNNGG 4 cut(s) 55, 121, 193, 208
AjnI CCWGG 1 cut(s) 54
AluBI AGCT 4 cut(s) 40, 70, 131, 266
AluI AGCT 4 cut(s) 40, 70, 131, 266
AoxI GGCC 5 cut(s) 3, 75, 97, 183, 195
ApeKI GCWGC 7 cut(s) 67, 92, 128, 239, 263, 299, 350
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 4 cut(s) 58, 76, 184, 211
AsuC2I CCSGG 3 cut(s) 157, 172, 203
AvaII GGWCC 2 cut(s) 58, 211
BalI TGGCCA 1 cut(s) 197
BbvI GCAGC 7 cut(s) 79, 104, 140, 226, 275, 311, 337
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 276
BciT130I CCWGG 1 cut(s) 56
BcnI CCSGG 3 cut(s) 157, 172, 203
BfaI CTAG 1 cut(s) 230
BglI GCCNNNNNGGC 1 cut(s) 105
BisI GCNGC 8 cut(s) 68, 93, 129, 240, 264, 292, 300, 351
BlsI GCNGC 8 cut(s) 69, 94, 130, 241, 265, 293, 301, 352
Bme1390I CCNGG 4 cut(s) 56, 157, 172, 203
Bme18I GGWCC 2 cut(s) 58, 211
BmgT120I GGNCC 4 cut(s) 58, 76, 184, 211
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 4 cut(s) 56, 157, 172, 203
BpuMI CCSGG 3 cut(s) 157, 172, 203
BsaAI YACGTR 1 cut(s) 346
BsaJI CCNNGG 2 cut(s) 120, 201
BsaWI WCCGGW 1 cut(s) 223
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 121, 193, 208
Bse118I RCCGGY 2 cut(s) 95, 235
Bse1I ACTGG 3 cut(s) 61, 109, 312
BseBI CCWGG 1 cut(s) 56
BseDI CCNNGG 2 cut(s) 120, 201
BseGI GGATG 1 cut(s) 327
BseLI CCNNNNNNNGG 4 cut(s) 55, 121, 193, 208
BseNI ACTGG 3 cut(s) 61, 109, 312
BseRI GAGGAG 2 cut(s) 73, 76
BseXI GCAGC 7 cut(s) 79, 104, 140, 226, 275, 311, 337
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 5 cut(s) 5, 77, 99, 185, 197
BsiSI CCGG 7 cut(s) 96, 156, 171, 203, 224, 236, 282
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 4 cut(s) 55, 121, 193, 208
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 5 cut(s) 5, 77, 99, 185, 197
Bsp143I GATC 1 cut(s) 7
BspACI CCGC 5 cut(s) 90, 120, 122, 285, 291
BspANI GGCC 5 cut(s) 5, 77, 99, 185, 197
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 78
BsrFI RCCGGY 2 cut(s) 95, 235
BsrI ACTGG 3 cut(s) 61, 109, 312
BssAI RCCGGY 2 cut(s) 95, 235
BssECI CCNNGG 2 cut(s) 120, 201
BssMI GATC 1 cut(s) 7
Bst2UI CCWGG 1 cut(s) 56
Bst4CI ACNGT 1 cut(s) 178
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 20, 97, 124
BstDSI CCRYGG 1 cut(s) 120
BstEII GGTNACC 1 cut(s) 365
BstF5I GGATG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 122
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 1 cut(s) 10
BstMBI GATC 1 cut(s) 7
BstMWI GCNNNNNNNGC 4 cut(s) 105, 128, 291, 308
BstNI CCWGG 1 cut(s) 56
BstPI GGTNACC 1 cut(s) 365
BstSCI CCNGG 4 cut(s) 54, 155, 170, 201
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 7 cut(s) 79, 104, 140, 226, 275, 311, 337
BsuRI GGCC 5 cut(s) 5, 77, 99, 185, 197
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 1 cut(s) 327
BtsIMutI CAGTG 2 cut(s) 68, 183
Cac8I GCNNGC 3 cut(s) 20, 97, 124
CfoI GCGC 1 cut(s) 128
Cfr10I RCCGGY 2 cut(s) 95, 235
Cfr13I GGNCC 4 cut(s) 58, 76, 184, 211
Cfr42I CCGCGG 1 cut(s) 123
Csp6I GTAC 1 cut(s) 233
CviAII CATG 2 cut(s) 193, 295
CviQI GTAC 1 cut(s) 233
DpnI GATC 1 cut(s) 9
DpnII GATC 1 cut(s) 7
EaeI YGGCCR 3 cut(s) 3, 97, 195
Eco47I GGWCC 2 cut(s) 58, 211
Eco91I GGTNACC 1 cut(s) 365
EcoO65I GGTNACC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 54
FaeI CATG 2 cut(s) 196, 298
FaiI YATR 4 cut(s) 194, 296, 327, 375
FaqI GGGAC 1 cut(s) 187
FatI CATG 2 cut(s) 192, 294
FauI CCCGC 2 cut(s) 115, 278
Fnu4HI GCNGC 8 cut(s) 68, 93, 129, 240, 264, 292, 300, 351
FokI GGATG 1 cut(s) 334
Fsp4HI GCNGC 8 cut(s) 68, 93, 129, 240, 264, 292, 300, 351
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 127
GluI GCNGC 8 cut(s) 68, 93, 129, 240, 264, 292, 300, 351
HaeIII GGCC 5 cut(s) 5, 77, 99, 185, 197
HapII CCGG 7 cut(s) 96, 156, 171, 203, 224, 236, 282
HhaI GCGC 1 cut(s) 128
Hin1II CATG 2 cut(s) 196, 298
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HpaI GTTAAC 1 cut(s) 162
HpaII CCGG 7 cut(s) 96, 156, 171, 203, 224, 236, 282
Hpy166II GTNNAC 1 cut(s) 162
Hpy188III TCNNGA 3 cut(s) 115, 145, 256
Hpy8I GTNNAC 1 cut(s) 162
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 128, 291, 308
HpySE526I ACGT 1 cut(s) 345
Hsp92II CATG 2 cut(s) 196, 298
HspAI GCGC 1 cut(s) 126
KroI GCCGGC 1 cut(s) 95
KroNI GCCGGC 1 cut(s) 97
KspAI GTTAAC 1 cut(s) 162
KspI CCGCGG 1 cut(s) 123
Kzo9I GATC 1 cut(s) 7
LmnI GCTCC 1 cut(s) 45
Lsp1109I GCAGC 7 cut(s) 79, 104, 140, 226, 275, 311, 337
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 341, 365
MalI GATC 1 cut(s) 9
MboI GATC 1 cut(s) 7
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 1 cut(s) 390
MluNI TGGCCA 1 cut(s) 197
MnlI CCTC 4 cut(s) 91, 94, 97, 197
Mox20I TGGCCA 1 cut(s) 197
MroNI GCCGGC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 197
MseI TTAA 2 cut(s) 161, 167
Msp20I TGGCCA 1 cut(s) 197
MspA1I CMGCKG 4 cut(s) 70, 122, 131, 266
MspI CCGG 7 cut(s) 96, 156, 171, 203, 224, 236, 282
MspR9I CCNGG 4 cut(s) 56, 157, 172, 203
MvaI CCWGG 1 cut(s) 56
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 4 cut(s) 105, 128, 291, 308
NaeI GCCGGC 1 cut(s) 97
NciI CCSGG 3 cut(s) 157, 172, 203
NdeII GATC 1 cut(s) 7
NgoMIV GCCGGC 1 cut(s) 95
NlaIII CATG 2 cut(s) 196, 298
NlaIV GGNNCC 1 cut(s) 78
PdiI GCCGGC 1 cut(s) 97
PkrI GCNGC 8 cut(s) 69, 94, 130, 241, 265, 293, 301, 352
Ppu21I YACGTR 1 cut(s) 346
Psp6I CCWGG 1 cut(s) 54
PspEI GGTNACC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 54
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 4 cut(s) 58, 76, 184, 211
PsrI GAACNNNNNNTAC 2 cut(s) 365, 397
PvuII CAGCTG 3 cut(s) 70, 131, 266
RsaI GTAC 1 cut(s) 234
RsaNI GTAC 1 cut(s) 233
SacII CCGCGG 1 cut(s) 123
SaqAI TTAA 2 cut(s) 161, 167
SatI GCNGC 8 cut(s) 68, 93, 129, 240, 264, 292, 300, 351
Sau3AI GATC 1 cut(s) 7
Sau96I GGNCC 4 cut(s) 58, 76, 184, 211
ScrFI CCNGG 4 cut(s) 56, 157, 172, 203
SetI ASST 5 cut(s) 42, 72, 133, 268, 348
Sfr303I CCGCGG 1 cut(s) 123
SgrBI CCGCGG 1 cut(s) 123
SinI GGWCC 2 cut(s) 58, 211
Sse9I AATT 1 cut(s) 390
SsiI CCGC 5 cut(s) 90, 120, 122, 285, 291
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 4 cut(s) 54, 155, 170, 201
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 348
TaqI TCGA 1 cut(s) 338
TasI AATT 1 cut(s) 390
TauI GCSGC 1 cut(s) 294
Tru1I TTAA 2 cut(s) 161, 167
Tru9I TTAA 2 cut(s) 161, 167
TscAI CASTG 2 cut(s) 68, 183
TseI GCWGC 7 cut(s) 67, 92, 128, 239, 263, 299, 350
TspRI CASTG 2 cut(s) 68, 183
VpaK11BI GGWCC 2 cut(s) 58, 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.