pycom08g09410

ultraviolet-B receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013356
Physical Location & Seq
Forward (+)
7687790 .. 7689888
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g09410.1

Sequence Viewer

Length: 1323 bp
ATGGAAGAGGAAAGCGTCACTCCTCCGAACGGCGACAACTTGTCTCAAAAGGTGGTGGCGGTGGCCGCCGGCGAAGCACACACTCTGGCTCTGACCGGCGATGGGTTTGTGTATTCGTGGGGAAGAGGCATGTTTGGGCGGCTCGGGACCGGTTCGGAGTCCGACGAGCTCTCCCCGGTTCGAATAAAGTTCAATGATCCTCGCAGTGAAGAAGGAGCGAGGCCCAAATTTGTGGGAGTTGCCGCCGGTGCTTACCACAGTCTTGCTCTTGCTGGTCAGTTTATGATTGCTTTTCTTGAGGTGGCCTCTGCAACGGAGAGTGACGTGCCACTAAAGGTTTGTTCTGTTAAAGCTGGAGGGATGATGTCCCTAGCAATTGATAGCATTGGTGTCCTCTGGGTGTGGGGAAATTTCCCACACGAAAGCAGCAGCAATGAAGGTGGATTCTCGCTGATTAGCAGTTGCAACCCAACTCCTGTTTGGGATTTCTATGGCCACGTTGTTGTGAAGGTGGCATGTGGAAATGAGCATGTTGTGGCCCTGGTTTCTGCCGGAGAAGCATATAAAGGTGAAGATCTTGTTTGCTTCTCTTGGGGCGGTAACGGCCATGGCCAGTTAGGCCTGGGGGATAAAGGGAGCAGGAATCGTCCCCAAATAGTAGAACTATTTGATCACGACTCCCCTTTCGCAGTTTATGAGGTAGCATGTGGTGCTTTTCACACAGCTTTGCTTACCCACAGAAAAAGCCCAAATGACACATTGGAAAGCATGTGTTGGACGTTCGGCCTTGGAGAAAATGGGCAGCTCGGGCATGGAACCACCGAAAGTGGATTGTCTCCTGAACCTGTGAAAGAATTGCCACAGAATGTATACCTGGTGTCTCTTGACTGTGGCTTATTTCACACCAGCGTTGTTTCATCTGCCGGAGATGTGTGGTCGTGGGGAATGGAGAAGGGTCTTGGCCTATGCCGAGATGCTACTTTTAGCGAGACTGATTCAGGGGATGCCATCTCTCCCCTACAGATTAACGGATTGAATGGAACAAAATTTCAGGATCCAATCCAAGTTGTCTGTGGGGCAGCTCATACTGTTCTAGTTGCAAATGATGGATTTAAGCTTTGGTCTTGGGGGAGAGGAAGGAGTGGGGTTCTTGGAAATGGTAAGACGATTGACTGTTATTCTCCTGCTGAGGTCTTCTGGCCTCCTCTTACAGCGGATTTCAAACAAGAAGAATTAAACACTGTCGATAAGCGAAAACCTATTGATGATGGAGATAACAGAGGGGAAAGAAATTTGACAGTAGACGCCGAAGCAGAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

441

Amino Acids

46.92

Weight (kDa)

4.78

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 6 - 91 1e-15 RCC1-like domain
RCC1_2 PF13540 18 - 43 1.3e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 34 - 90 5.2e-13 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 107 - 399 8.5e-45 RCC1-like domain
RCC1 PF00415 195 - 244 6.5e-11 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 258 - 304 1.2e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013356)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11580 AT5G11580
fragaria_vesca FvH4_2g35490
malus_domestica MD15G1093100.v1.1
prunus_persica Prupe.1G447900_v2.0.a1
pyrus_communis pycom08g09410
rosa_chinensis RchiOBHm_Chr6g0306311 RchiOBHm_Chr6g0306371
rosa_laevigata RLG00000010803
rosa_multiflora Rmu_sc0003473.1_g000007
rosa_roxburghii Rroxscaffold_7G00161880
rosa_rugosa Rorug06G0348600 Rorug06G0348700 Rorug06G0352100
rosa_samantha Rh6AG461100 Rh6DG461900 Rh6DG465400
rosa_wichuraiana Rw6G040180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 870, 1302
AciI CCGC 6 cut(s) 59, 66, 139, 243, 597, 1214
AclWI GGATC 3 cut(s) 191, 1049, 1062
AcoI YGGCCR 4 cut(s) 63, 493, 604, 610
AcsI RAATTY 4 cut(s) 227, 409, 1046, 1291
AcyI GRCGYC 1 cut(s) 1305
AfiI CCNNNNNNNGG 2 cut(s) 29, 102
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 3 cut(s) 193, 1036, 1222
AhdI GACNNNNNGTC 1 cut(s) 40
AjiI CACGTC 1 cut(s) 325
AjnI CCWGG 3 cut(s) 540, 621, 873
AluBI AGCT 6 cut(s) 169, 353, 725, 805, 1082, 1117
AluI AGCT 6 cut(s) 169, 353, 725, 805, 1082, 1117
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 4 cut(s) 48, 840, 885, 983
AlwI GGATC 3 cut(s) 191, 1049, 1062
Ama87I CYCGRG 2 cut(s) 143, 806
ApeKI GCWGC 4 cut(s) 426, 429, 802, 1079
ApoI RAATTY 4 cut(s) 227, 409, 1046, 1291
ArsI GACNNNNNNTTYG 2 cut(s) 668, 700
AsiGI ACCGGT 1 cut(s) 149
AspS9I GGNCC 3 cut(s) 147, 222, 538
AsuC2I CCSGG 1 cut(s) 176
AsuHPI GGTGA 1 cut(s) 581
AsuII TTCGAA 1 cut(s) 181
AvaI CYCGRG 2 cut(s) 143, 806
AvaII GGWCC 1 cut(s) 147
BalI TGGCCA 2 cut(s) 495, 612
BamHI GGATCC 1 cut(s) 1054
BanII GRGCYC 1 cut(s) 171
BbsI GAAGAC 1 cut(s) 1186
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 1188
BbvI GCAGC 4 cut(s) 438, 441, 814, 1091
BccI CCATC 4 cut(s) 95, 1016, 1100, 1262
BceAI ACGGC 2 cut(s) 46, 619
BciT130I CCWGG 3 cut(s) 542, 623, 875
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 1 cut(s) 176
BcoDI GTCTC 4 cut(s) 48, 840, 885, 983
BfaI CTAG 2 cut(s) 371, 1094
BfmI CTRYAG 1 cut(s) 1019
BglI GCCNNNNNGGC 1 cut(s) 618
BglII AGATCT 1 cut(s) 574
BisI GCNGC 7 cut(s) 66, 140, 243, 427, 430, 803, 1080
BlsI GCNGC 7 cut(s) 67, 141, 244, 428, 431, 804, 1081
Bme1390I CCNGG 4 cut(s) 176, 542, 623, 875
Bme18I GGWCC 1 cut(s) 147
BmeRI GACNNNNNGTC 1 cut(s) 40
BmeT110I CYCGRG 2 cut(s) 143, 806
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 3 cut(s) 147, 222, 538
BmiI GGNNCC 3 cut(s) 148, 817, 1056
BmrFI CCNGG 4 cut(s) 176, 542, 623, 875
BmsI GCATC 2 cut(s) 964, 994
BpiI GAAGAC 1 cut(s) 1186
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 1188
Bpu14I TTCGAA 1 cut(s) 181
BpuEI CTTGAG 1 cut(s) 317
BpuMI CCSGG 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 1305
BsaJI CCNNGG 5 cut(s) 174, 540, 607, 622, 787
BsaWI WCCGGW 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 102
Bse118I RCCGGY 4 cut(s) 68, 95, 149, 245
Bse1I ACTGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 439
BseBI CCWGG 3 cut(s) 542, 623, 875
BseDI CCNNGG 5 cut(s) 174, 540, 607, 622, 787
BseGI GGATG 2 cut(s) 366, 1009
BseLI CCNNNNNNNGG 2 cut(s) 29, 102
BseMI GCAATG 1 cut(s) 439
BseMII CTCAG 1 cut(s) 1179
BseNI ACTGG 1 cut(s) 613
BseRI GAGGAG 2 cut(s) 12, 1194
BseXI GCAGC 4 cut(s) 438, 441, 814, 1091
BshTI ACCGGT 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 171
BsiHKCI CYCGRG 2 cut(s) 143, 806
BsiSI CCGG 7 cut(s) 69, 96, 150, 176, 246, 552, 924
BslFI GGGAC 3 cut(s) 160, 352, 633
BslI CCNNNNNNNGG 2 cut(s) 29, 102
BsmAI GTCTC 4 cut(s) 48, 840, 885, 983
BsmFI GGGAC 3 cut(s) 160, 352, 633
BsoBI CYCGRG 2 cut(s) 143, 806
Bsp119I TTCGAA 1 cut(s) 181
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 4 cut(s) 196, 574, 670, 1054
Bsp19I CCATGG 1 cut(s) 607
BspACI CCGC 6 cut(s) 59, 66, 139, 243, 597, 1214
BspCNI CTCAG 1 cut(s) 1180
BspLI GGNNCC 3 cut(s) 148, 817, 1056
BspPI GGATC 3 cut(s) 191, 1049, 1062
BspT104I TTCGAA 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 439
BsrFI RCCGGY 4 cut(s) 68, 95, 149, 245
BsrI ACTGG 1 cut(s) 613
BssAI RCCGGY 4 cut(s) 68, 95, 149, 245
BssECI CCNNGG 5 cut(s) 174, 540, 607, 622, 787
BssMI GATC 4 cut(s) 196, 574, 670, 1054
BssNAI GTATAC 1 cut(s) 871
BssNI GRCGYC 1 cut(s) 1305
BssT1I CCWWGG 2 cut(s) 607, 787
Bst1107I GTATAC 1 cut(s) 871
Bst2UI CCWGG 3 cut(s) 542, 623, 875
Bst4CI ACNGT 6 cut(s) 260, 890, 1090, 1175, 1243, 1300
Bst6I CTCTTC 1 cut(s) 118
BstACI GRCGYC 1 cut(s) 1305
BstAPI GCANNNNNTGC 1 cut(s) 710
BstBI TTCGAA 1 cut(s) 181
BstC8I GCNNGC 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 1188
BstDSI CCRYGG 1 cut(s) 607
BstF5I GGATG 2 cut(s) 366, 1009
BstKTI GATC 4 cut(s) 199, 577, 673, 1057
BstMAI GTCTC 4 cut(s) 48, 840, 885, 983
BstMBI GATC 4 cut(s) 196, 574, 670, 1054
BstMWI GCNNNNNNNGC 8 cut(s) 65, 74, 248, 557, 603, 618, 710, 808
BstNI CCWGG 3 cut(s) 542, 623, 875
BstNSI RCATGY 5 cut(s) 133, 519, 533, 708, 772
BstSCI CCNGG 4 cut(s) 174, 540, 621, 873
BstSFI CTRYAG 1 cut(s) 1019
BstV1I GCAGC 4 cut(s) 438, 441, 814, 1091
BstV2I GAAGAC 1 cut(s) 1186
BstX2I RGATCY 2 cut(s) 574, 1054
BstXI CCANNNNNNTGG 1 cut(s) 232
BstYI RGATCY 2 cut(s) 574, 1054
BstZ17I GTATAC 1 cut(s) 871
BtgI CCRYGG 1 cut(s) 607
BtgZI GCGATG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 2 cut(s) 366, 1009
BtsI GCAGTG 1 cut(s) 211
BtsIMutI CAGTG 2 cut(s) 211, 1239
Cac8I GCNNGC 1 cut(s) 70
Cfr10I RCCGGY 4 cut(s) 68, 95, 149, 245
Cfr13I GGNCC 3 cut(s) 147, 222, 538
CseI GACGC 2 cut(s) 4, 1313
CsiI ACCWGGT 1 cut(s) 873
CspAI ACCGGT 1 cut(s) 149
CspCI CAANNNNNGTGG 2 cut(s) 421, 456
CviAII CATG 7 cut(s) 130, 516, 530, 608, 705, 769, 812
DdeI CTNAG 1 cut(s) 1188
DpnI GATC 4 cut(s) 198, 576, 672, 1056
DpnII GATC 4 cut(s) 196, 574, 670, 1054
DriI GACNNNNNGTC 1 cut(s) 40
EaeI YGGCCR 4 cut(s) 63, 493, 604, 610
Eam1104I CTCTTC 1 cut(s) 118
Eam1105I GACNNNNNGTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 118
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 2 cut(s) 607, 787
Eco147I AGGCCT 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 171
Eco47I GGWCC 1 cut(s) 147
Eco53kI GAGCTC 1 cut(s) 169
Eco88I CYCGRG 2 cut(s) 143, 806
EcoICRI GAGCTC 1 cut(s) 169
EcoRII CCWGG 3 cut(s) 540, 621, 873
EcoT14I CCWWGG 2 cut(s) 607, 787
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 2 cut(s) 607, 787
FaeI CATG 7 cut(s) 133, 519, 533, 611, 708, 772, 815
FaqI GGGAC 3 cut(s) 160, 352, 633
FatI CATG 7 cut(s) 129, 515, 529, 607, 704, 768, 811
FbaI TGATCA 1 cut(s) 670
FblI GTMKAC 2 cut(s) 870, 1302
Fnu4HI GCNGC 7 cut(s) 66, 140, 243, 427, 430, 803, 1080
FokI GGATG 2 cut(s) 373, 1016
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 7 cut(s) 66, 140, 243, 427, 430, 803, 1080
FspBI CTAG 2 cut(s) 371, 1094
GluI GCNGC 7 cut(s) 66, 140, 243, 427, 430, 803, 1080
GsuI CTGGAG 1 cut(s) 375
HapII CCGG 7 cut(s) 69, 96, 150, 176, 246, 552, 924
HgaI GACGC 2 cut(s) 4, 1313
Hin1I GRCGYC 1 cut(s) 1305
Hin1II CATG 7 cut(s) 133, 519, 533, 611, 708, 772, 815
HindIII AAGCTT 1 cut(s) 1115
HinfI GANTC 5 cut(s) 158, 444, 643, 677, 995
HpaII CCGG 7 cut(s) 69, 96, 150, 176, 246, 552, 924
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 2 cut(s) 871, 1303
Hpy188I TCNGA 4 cut(s) 27, 93, 157, 163
Hpy188III TCNNGA 6 cut(s) 145, 296, 674, 839, 884, 1052
Hpy8I GTNNAC 2 cut(s) 871, 1303
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 5 cut(s) 206, 431, 502, 946, 1131
HpyCH4III ACNGT 6 cut(s) 260, 890, 1090, 1175, 1243, 1300
HpyCH4IV ACGT 3 cut(s) 324, 498, 779
HpyCH4V TGCA 3 cut(s) 311, 465, 1100
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 74, 248, 557, 603, 618, 710, 808
HpyF3I CTNAG 1 cut(s) 1188
HpySE526I ACGT 3 cut(s) 324, 498, 779
Hsp92I GRCGYC 1 cut(s) 1305
Hsp92II CATG 7 cut(s) 133, 519, 533, 611, 708, 772, 815
KroI GCCGGC 1 cut(s) 68
KroNI GCCGGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 4 cut(s) 196, 574, 670, 1054
LmnI GCTCC 2 cut(s) 215, 636
Lsp1109I GCAGC 4 cut(s) 438, 441, 814, 1091
LweI GCATC 2 cut(s) 964, 994
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 2 cut(s) 371, 1094
MaeII ACGT 3 cut(s) 324, 498, 779
MaeIII GTNAC 3 cut(s) 16, 320, 599
MalI GATC 4 cut(s) 198, 576, 672, 1056
MboI GATC 4 cut(s) 196, 574, 670, 1054
MboII GAAGA 6 cut(s) 17, 135, 221, 584, 1186, 1241
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 2 cut(s) 574, 1054
MhlI GDGCHC 1 cut(s) 171
MlsI TGGCCA 2 cut(s) 495, 612
MluCI AATT 7 cut(s) 227, 375, 409, 854, 1046, 1232, 1291
MluNI TGGCCA 2 cut(s) 495, 612
MlyI GAGTC 2 cut(s) 167, 671
MmeI TCCRAC 2 cut(s) 186, 755
Mox20I TGGCCA 2 cut(s) 495, 612
MreI CGCCGGCG 1 cut(s) 68
MroNI GCCGGC 1 cut(s) 68
MscI TGGCCA 2 cut(s) 495, 612
MseI TTAA 4 cut(s) 348, 1026, 1113, 1235
Msp20I TGGCCA 2 cut(s) 495, 612
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 7 cut(s) 69, 96, 150, 176, 246, 552, 924
MspR9I CCNGG 4 cut(s) 176, 542, 623, 875
MunI CAATTG 1 cut(s) 375
MvaI CCWGG 3 cut(s) 542, 623, 875
MwoI GCNNNNNNNGC 8 cut(s) 65, 74, 248, 557, 603, 618, 710, 808
NaeI GCCGGC 1 cut(s) 70
NciI CCSGG 1 cut(s) 176
NcoI CCATGG 1 cut(s) 607
NdeII GATC 4 cut(s) 196, 574, 670, 1054
NgoMIV GCCGGC 1 cut(s) 68
NlaIII CATG 7 cut(s) 133, 519, 533, 611, 708, 772, 815
NlaIV GGNNCC 3 cut(s) 148, 817, 1056
NmeAIII GCCGAG 1 cut(s) 995
NmuCI GTSAC 2 cut(s) 16, 320
NspI RCATGY 5 cut(s) 133, 519, 533, 708, 772
NspV TTCGAA 1 cut(s) 181
PceI AGGCCT 1 cut(s) 621
PdiI GCCGGC 1 cut(s) 70
PfeI GAWTC 3 cut(s) 444, 643, 995
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 7 cut(s) 67, 141, 244, 428, 431, 804, 1081
PleI GAGTC 2 cut(s) 166, 671
PpsI GAGTC 2 cut(s) 166, 671
Psp124BI GAGCTC 1 cut(s) 171
Psp6I CCWGG 3 cut(s) 540, 621, 873
PspGI CCWGG 3 cut(s) 540, 621, 873
PspN4I GGNNCC 3 cut(s) 148, 817, 1056
PspPI GGNCC 3 cut(s) 147, 222, 538
PsuI RGATCY 2 cut(s) 574, 1054
SacI GAGCTC 1 cut(s) 171
SaqAI TTAA 4 cut(s) 348, 1026, 1113, 1235
SatI GCNGC 7 cut(s) 66, 140, 243, 427, 430, 803, 1080
Sau3AI GATC 4 cut(s) 196, 574, 670, 1054
Sau96I GGNCC 3 cut(s) 147, 222, 538
SchI GAGTC 2 cut(s) 167, 671
ScrFI CCNGG 4 cut(s) 176, 542, 623, 875
SduI GDGCHC 1 cut(s) 171
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 2 cut(s) 964, 994
SfcI CTRYAG 1 cut(s) 1019
SfiI GGCCNNNNNGGCC 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 181
SgrAI CRCCGGYG 2 cut(s) 68, 245
SinI GGWCC 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 7 cut(s) 227, 375, 409, 854, 1046, 1232, 1291
SseBI AGGCCT 1 cut(s) 621
SsiI CCGC 6 cut(s) 59, 66, 139, 243, 597, 1214
SspMI CTAG 2 cut(s) 371, 1094
SstI GAGCTC 1 cut(s) 171
StuI AGGCCT 1 cut(s) 621
StyD4I CCNGG 4 cut(s) 174, 540, 621, 873
StyI CCWWGG 2 cut(s) 607, 787
TaaI ACNGT 6 cut(s) 260, 890, 1090, 1175, 1243, 1300
TaiI ACGT 3 cut(s) 327, 501, 782
TaqI TCGA 2 cut(s) 181, 1245
TasI AATT 7 cut(s) 227, 375, 409, 854, 1046, 1232, 1291
TauI GCSGC 3 cut(s) 68, 142, 245
TfiI GAWTC 3 cut(s) 444, 643, 995
Tru1I TTAA 4 cut(s) 348, 1026, 1113, 1235
Tru9I TTAA 4 cut(s) 348, 1026, 1113, 1235
TscAI CASTG 2 cut(s) 211, 1246
TseFI GTSAC 2 cut(s) 16, 320
TseI GCWGC 4 cut(s) 426, 429, 802, 1079
Tsp45I GTSAC 2 cut(s) 16, 320
TspDTI ATGAA 2 cut(s) 450, 906
TspGWI ACGGA 2 cut(s) 329, 1044
TspRI CASTG 2 cut(s) 211, 1246
VpaK11BI GGWCC 1 cut(s) 147
XapI RAATTY 4 cut(s) 227, 409, 1046, 1291
XceI RCATGY 5 cut(s) 133, 519, 533, 708, 772
XcmI CCANNNNNNNNNTGG 2 cut(s) 477, 1070
XmiI GTMKAC 2 cut(s) 870, 1302
XspI CTAG 2 cut(s) 371, 1094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.